Professional Documents
Culture Documents
S. Saengamnatdej, PhD.
for 499305 (2009/2)
April 25, 2009
Goals
Specimen Processing
Collection of Specimens
Methods
Goals
1. To confirm the diagnosis (acute or chronic infection).
2. To determine appropriate antiviral therapy.
3. To define the course of disease.
4. To determine the immune status.
5. To establish public health measures to prevent the spread.
6. To study the evolution & epidemiology of viral infections.
Specimen Flow Diagram
Request Report
Macroscopic & Microscopic
Transport Examination Initial (via phone)
Preliminary
Cultivation, isolation Identification
(day after receipt)
viruses, fungi &some bacteria
may take longer time.
Identification;
antimicrobial sensitivity testing Final (2nd day)
Further identification;
Supplementary
typing; toxin production;
Slack, R.C.B. (2006) further sensitivities
Collection of Specimens
- Avoid contamination
(commensals or external sources)
- In a transport-durable, leak-proof,
sterile container.
- Labeled correctly.
- Kept separately from food and drug.
- Transported on ice (4 ºC) without delay.
(frozen, if undelivered for weeks.)
GI specimens Vomitus,
Reovirus, Rotavirus, Stool, Rectal swab (meningitis during
Adenovirus, Norwalk, Calicivirus summer— enterovirus)
Methods of Specimen Collection (Cont.)
CNS CSF
(ex: CNS disease after parotitis—mumps
or meningitis during summer—enterovirus)
Brain biopsy: rabies
Urine
Rubella, Measles, Adenovirus, CMV,
Mumps (ex: CNS disease after parotitis, Maculopapular rash)
Timing : Viral shedding
For examples,
Respiratory viruses
shed for only 3-7 days
3.Cytology
CPEs
5.Electron Microscopy
Notes on Serology Methods.