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57th International Congress and Annual Meeting of the GA | August 16 20, 2009, Geneva, Switzerland

antimicrobial properties of U. longiflora. However, further studies are required to identify compounds responsible for these activities. References: [1] Heitzman, M.E. et al. (2005) Phytochemistry 66:5 29. [2] Kam, T.S. et al. (1992) Phytochemistry 31:2031 2034.

PH12

Antihyperglycemic activity of aqueous stem bark extract of Eugenia jambolana in alloxanized rats
Yele SU, Veeranjaneyulu A Dept. of Pharmacology, School of Pharmacy & Technology Management, NMIMS University, Mumbai-56, India

PH10

Malva sylvestris water extract: A potential anti-Inflammatory and anti-ulcerogenic remedy


Sleiman NH, Daher CF School of Arts and Sciences, Natural Sciences Department, Lebanese American University, PO Box 36, Byblos, Lebanon

Malva sylvestris, family Malvaceae, has been grown as a medicinal plant and pot herb since Roman times. It is found in subtropical and temperate latitude of both hemispheres. The present study investigates the role of the aqueous extract of its aerial part upon lipemia, glycemia, inflammation and gastric ulcer using rats as a model. After one month of extract intake via drinking water (100, 400 and 800 mg/kg body weight) the 400 and 800 mg/kg body weight doses resulted in significant increase in serum triglyceride, while other lipid and glycemic parameters and liver enzyme activities (AST, ALT, LDH, ALP) were unaffected. About 10 % increase in stool water content was observed at highest dose used. Doses of 50, 100, 250 and 500 mg/kg body weight were used in acute and chronic inflammation models induced by carrageenan and formalin respectively [1]. Significant anti-inflammatory activity was observed at most doses used with an optimum inhibition at 100 mg/kg body weight (60 % inhibition) in both models. Protection against ethanol-induced gastric ulcer was investigated [2]. Results showed maximum protection (37%) at 500 mg/kg body weight, a value higher than that observed with cimetidine (30 %), a reference drug. The assessment of antibacterial activity against 11 bacterial hospital isolates and the antifungal effect against Candida albicans using disk diffusion technique showed no potentials in this respect. In conclusion, Malva sylvestris water extract showed no liver toxicity, and exhibited a positive effect on ulcer and inflammation with relatively a neutral effect on lipemia and glycemia. Acknowledgments: Mr. Jean Karam. References: [1] Jose, N. et al. (2004) Phytother. Res. 18:43 46. [2] Alkofahi, A. and Atta, A.H. (1999) J. Ethnopharmacol. 67:341 345.

The antidiabetic potential of the aqueous stem bark extract of Eugenia jambolana (EJ) Linn. (Myrtaceae) was evaluated in the Alloxan induced diabetic model [1]. Eugenia jambolana Linn. a medicinal plant widely used in the traditional Ayurveda and Siddha systems of medicine for the treatment of diabetes mellitus and several other diseases [2]. Graded doses of the aqueous stem bark extract of EJ were administered to normal and experimental diabetic rats for 28 days. Blood glucose levels were evaluated on the 7th, 14th, 21st and 28th days. Doses of 500 and 1000 mg/kg body weight of both extracts produced significant (p < 0.05) hypoglycemic activity in alloxanized rats when compared with diabetic control. The biochemical parameters studied were serum triglycerides, cholesterol, high density lipoproteins (HDL), and low density lipoproteins (LDL), and glycosylated hemoglobin level. In addition, changes in body weight was assessed in the EJ extract treated diabetic rats were compared with diabetic control and normal animals. Significant results were observed in the estimated parameters, thereby justifying the use of the plant in the indigenous system of medicine. References: [1] Madhavan, V. et al. (2007) Pharm. Biol. 45:18 21. [2] Nadkarni, A.K. and Nadkarni, K.M. (2007) Indian Materia Medica, Bombay Popular Prakashan, India.

PH13

In-vivo antidiabetic effect of Cistus laurifolius L. leaves


Aslan M, Orhan N, Sezik E Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, 06330 Hipodrom-Ankara, Turkey

PH11

El-Toumy SA1, Farrag AH2, Ellithey MM3, Korien KM4 1 Chemistry of Tannins Department; 2Pathology Department; 3 Pharmcology Department; 4Medical Physiology, National Research Center, El-Bohouth Str., Dokki, 12622 Cairo, Egypt Gastric and duodenal ulcers are illnesses that affect a considerable number of people in the world and they are induced by several factors, for example, stress, smoking, nutritional deficiencies and ingestion of nonsteroidal-antiinflammatory drugs. Plant extracts are some of the most attractive sources for new drugs and have been shown to produce promising results in the treatment of gastric ulcer [1]. The object of the study deals with the antiulcerogenic effect of an hdroacoholic extracts obtained from of Acacia nilotica leaves and Retama raetam seeds was investigated using indomethacin-induced ulcer model in rats. Antiulcerogenic activities of hydroacoholic extract of A. nilotica (30 mg/kg) and hydroalcoholic extract of R. raetam (25 mg/kg) were determined by comparing the negative treated only with (indomethacin) and positive (ranitidine) control group. Although the dose of the hydroacoholic extract of A. nilotica and R. raetam showed significant antiulcerogenic activity as compared to negative control, the highest activity was observed with mixed (30 mg/kg) of A. nilotica and (25 mg/kg) of R. raetam (81%). The activities of enzymes superoxide dismutase (SOD), catalase (CAT) and gluthione-S-transferase (GST) were determined in the stomach tissues of rats and compared with those of negative and positive control groups. The enzymes activities in indomethacin-administrated were reduced significantly SOD (38.50 4.16), CAT (43.20 3.09), GST (22.80 2.25) and increased by the plants extracts SOD (48.90 4.07), CAT (37.50 3.26), GST (32.00 2.06) and ranitidine SOD (52.30 3.51), CAT (41.90 2.67), GST (23.70 2.2.14), (p < 0.05). Phytochemical investigation of Acacia nilotica leaves and Retama raetam seeds afforded of catechin gallate esters and isoflavonoids C-glycosides derivatives. The presence of these phenolic compounds may probably explain the antiulcerogenic and antioxidant effect of the plant extracts. Reference: [1]Toma, W. et al. (2002) Biol. Pharm. Bull. 25:1151 1155.

Effect of plant derived-phenolic extracts on antioxidant enzyme activity and mucosal damage caused by indomethacin in rats

The genus Cistus (Cistaceae) is one of the characteristic genera of the Mediterranean region, colonizing degraded areas [1]. In Turkish folk medicine, various parts of Cistus laurifolius L. are used to treat peptic ulcer and various types of pains, i. e., rheumatic, back pain, etc. It has been used for the treatment of stomach ache and gastric ulcer in the folk medicine since the time of Dioscorides, i. e., for at least 2000 years in Anatolia. The tea prepared from the leaves is used to decrease symptoms of diabetes hypoglycemic [2,3]. In the present study, the hypoglycemic and antihyperglycemic effects of water and ethanol extracts of C. laurifolius were evaluated by using in vivo methods in normal, glucose loaded hyperglycemic and streptozocin-induced diabetic rats. Diabetes was induced by intraperitoneal injection of streptozocin at the dose of 55 mg/kg. Blood glucose levels of animals were measured by the glucose oxidase method. Tolbutamide (100 mg/kg) was used as reference drug. All data were compared to control group. Results indicated that blood glucose levels of the streptozocin induced diabetic rats were decreased by loaded ethanol extract at the doses of 250 and 500 mg/kg as compared to control group (16 34 %). On the other hand all extracts tested in the experiments were found inactive totally on blood glucose level of normal rats. Water and ethanol extracts have shown a weak hypoglycemic effect in glucose loaded animals (11 20 %). The experimental data obtained from water and ethanol extracts of leaves confirmed the folkloric utilization. Acknowledgements: This study was financially supported by the Research Fund of Gazi University (Project no:EF-02/ 2003 02) References: [1] Attaguile, G. et al. (2000) Cell. Biol. Toxicol. 16:83 90. [2] Sezik, E. et al. (1991) J. Ethnopharmacol. 35:191 196. [3] Yesilada, E. et al. (1995) J. Ethnopharmacol. 46:133 152.

PH14

Antidiabetic effect and antioxidant potential of Rosa canina fruits


Orhan N, Aslan M, Hos bas S, Deliorman Orhan D Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, 06330 Hipodrom-Ankara, Turkey

Rosa canina L. fruits (Rosaceae) are used to treat diabetes in Anatolia traditionally [1,2,3]. In this study, the ethanol extract of R. canina fruits and its fractions were screened for their antioxidant, hypoglycaemic and antidiabetic activities. Two doses of ethanol extract (250 and 500 mg/kg) was administered to streptozotocin (STZ) induced diabetic rats for 7 days. The extract possessed a remarkable hypoglycemic effect at 250 mg/kg dose. Then it was fractionated through successive solvent extractions to yield chloroform fraction (CHCl3 Fr.), ethyl acetate fraction (EtOAc Fr.), n-butyl alcohol fraction (n-BuOH Fr.) and remaining water

& Korrekturexemplar: Verffentlichung (auch online), Vervielfltigung oder Weitergabe nicht erlaubt!
Planta Med 2009; 75: 8771095 Georg Thieme Verlag KG Stuttgart New York ISSN 0032-0943

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