(DMB 01)

M.Sc. (Previous) DEGREE EXAMINATION, JUNE 2010. First Year Microbiology Paper I INTRODUCTION MICROORGANISMS Time : Three hours Maximum : 100 marks

PART A (5 8 = 40 marks) Answer any FIVE of the following. 1. 2. 3. 4. 5. 6. 7. 8. Spontaneous generation. Louis Pasteur. Cyanobacteria. Mycoplasma. Chemistry of viruses. Viroids. Protozoa significance. Fungi reproduction.

PART B (4 15 = 60 marks) Answer all FOUR questions. 9. (a) Elaborate on the developments of vaccines. Or (b) Outline the ultra bacterial cell. structure of typical

10.

(a)

Explain the principles of bacterial taxonomy. Or

(b)

Write an essay on Agrobacterium and its importance. Describe the discovery of important viruses. Or

11.

(a)

(b)

Outline the ultrastructure and replication of T4. What is Ainsworth system and how it is useful in classifying fungi? Or

12.

(a)

(b)

Discuss the structure and replication of microalgae.

(DMB 01)

(DMB 02)
M.Sc. (Previous) DEGREE EXAMINATION, JUNE 2010. First Year Microbiology Paper II MICROBIOLOGICAL METHODS Time : Three hours Maximum : 100 marks

PART A (5 8 = 40 marks) Answer any FIVE of the following. 1. 2. 3. 4. 5. 6. 7. 8. Simple staining. Ethylene oxide. Bailing technique. MPN method. Plaque assay. Protoplast method. Beers Lambert Law. Autoradiography.

PART B (4 15 = 60 marks) Answer ALL questions. 9. (a) Describe the electron microscope and its uses. Or (b) Elaborate on the physical methods used for sterilization. How to isolate pure culture using the plating methods? Or (b) 11. (a) Discuss the methods of anaerobic culturing. Explain the methods used for isolation and purification of viruses. Or (b) What are chromatographic methods and discuss the HPLC? Write an essay chromatography. Or (b) Outline the pulse field gel electrophoresis and its uses. 2 on liquid scintillation

10.

(a)

12.

(a)

(DMB 02)

(DMB 03)
M.Sc. (Previous) DEGREE EXAMINATION, JUNE 2010. First Year Microbiology Paper III MICROBIAL PHYSIOLOGY AND BIOCHEMISTRY Time : Three hours Maximum : 100 marks

SECTION A (5 8 = 40 marks) Answer any FIVE of the following : All questions carry equal marks. 1. 2. 3. 4. 5. 6. Faciliated diffusion. Batch culture. Purple sulfur bacteria. Iron Oxidizers. Free energy. Kreb's cycle.

7. 8.

Mixed fermentation. Purine. SECTION B (4 15 = 60 marks) Answer all the FOUR questions. All questions carry equal marks.

9.

(a)

Discuss the role of macronutrients and micronutrients on growth. Or

(b)

Explain growth.

the

factors

affecting

bacterial

10.

(a)

Describe the mechanism of photosynthesis in anoxygenic bacteria. Or

(b)

Explain the characteristics and importance of Nitrate oxidizers and sulfur oxidizers. Describe the mechanisms of phosphorylation. Or

11.

(a)

(b)

Explain the steps involved in Glycolysis.

(DMB 03)

12.

(a)

Describe Michaelis - mention equation and mechanism of enzymes catalysis. Or

(b)

Explain the structure of Watson - Crick's model of DNA.

(DMB 03)

(DMB 04)
M.Sc. (Previous) DEGREE EXAMINATION, JUNE 2010. First Year Microbiology Paper IV ENVIRONMENTAL AND AGRICULTURAL MICROBIOLOGY Time : Three hours Maximum : 100 marks

SECTION A (5 8 = 40 marks) Answer any FIVE of the following. 1. 2. 3. 4. 5. 6. 7. 8. Phytoplankton. Air sampling. Soil components. Rhizosphere. Leg haemoglobin. Cron induction groups. Blast of Rice. Soft rot of Vegetables.

SECTION B (4 15 = 60 marks) Answer ALL questions. 9. (a) Describe the waterborne diseases. Or (b) Elaborate the role of Aero biology in relation to plant pathology. Discuss the decomposition. Or (b) How the carbon in transformed in the environment? Explain the symbiotic nitrogen fixation. Or (b) What are the types of Mycorliza and discuss the importance of VAM fungi? Outline the symptoms caused by plant pathogens. Or (b) Write an essay on biological control of plant pathogens. 2 soil organic matter

10.

(a)

11.

(a)

12.

(a)

(DMB 04)