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PHYSICAL AGENTS
Physical agents are usually preferred over chemical agents for performing
sterilization. Heat is the method of choice wherever possible. Both moist as well as
dry heat can be applied.
Moist Heat
This is heating in the presence of water. The best and widely used examples of this
technique are holder method of Pasteurization where 60°C for 30 minutes is
employed for sterilization and the 'flash' modification of the same wherein objects
are subjected to a temperature of 71.1°C for 15 seconds. Tyndallization is another
methodology in which steaming of the object is done for 30 minutes on each of the 3
consecutive days. The principle is that spores which survived the heating process
would germinate before the next thermal exposure and would then be killed. Dry
saturated steam acts as an excellent agent for
sterilization because of:
· High temperature
· Wealth of latent heat
· Ability to form water of condensation
· Instantaneous contraction in volume that occurs during condensation.
Superheated steam is not that effective because it is hotter than dry saturated steam
and the process is akin to dry heat which is not as efficient as moist heat.
Autoclaves are designed upon the principles of moist heat. The ideal time-
temperature relationship in moist heat and dry sterilization processes are as follows:
Process Temperature Holding period
Moist heat 121°C 15 minutes
(Autoclaving) 126°C 10 minutes
134°C 3 minutes
Dry Heat 160°C 120 minutes
170°C 60 minutes
IS0°C 30 minutes
Mechanism of Inactivation:
Microbial inactivation by dry heat is primarily an oxidation process. However, the
possibility of DNA damage is also now incriminated as one of the mechanisms. Dry
heat is employed for sterilization of glassware, glass syringes, oil and oily injections
and metal instruments. The thermal death point is the time required to kill all the
bacteria in a particular culture at a specified temperature. The decimal reduction
time, also known as the DRT or D value, is the length of time needed to kill 90 per
cent of the organisms in a given population at a specified temperature.
Ionizing Radiations
Ionizing radiations include X-rays, gamma rays and beta rays. These can induce
single stranded and sometimes double stranded breakdown of DNA.
Ultraviolet Radiations
Bacterial spores are more resistant to UV rays than the vegetative cells. Even viruses
are often more resistant than vegetative bacteria. Whereas Sarcina lutea is highly
sensitive to UV rays, Salmonella typhimurium is moderately sensitive and organisms
such as Escherichia coli, Staphylococcus and Proteus vulgaris are mildly sensitive to
the action of these rays.
Mechanism of Inactivation:
Because of the presence of UV rays, the sunlight has got bactericidal activity.
Sunlight can inactivate even spores provided the exposure is of prolonged duration.
The desert surfaces are found to be sterile not only because of immense dry heat but
also because of prolonged exposure to UV rays present in the sunlight
Sterilization by Filtration
IDEAL DISINFECTANT
Properties of an ideal disinfectant:
Broad spectrum: Should always have the widest possible antimicrobial
spectrum.
Fast acting: should have a rapidly lethal action on all vegetative forms and
spores of bacteria and fungi, protozoa and viruses.
Not affected by physical factors:
Nontoxic
Surface compatibility
Residual effect on treated surfaces
Easy to use
Odorless: an inoffensive odor would facilitate its routine use.
Economical: cost should not be prohibitively high.