SBP PERFECT SCORE BIOLOGY SET 4 (P3)

SBP PERFECT SCORE BIOLOGY SET 4

PAPER 3

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SBP PERFECT SCORE BIOLOGY SET 4 (P3) Question 1

1. An experiment to investigate the effects of temperature on the rate of an enzyme reaction is carried out by a group of student with the following steps: Step 1: Step 2: Step 3: Fifteen drops of iodine solution were dropped separately in the grooves of a white tile. 1ml of saliva was put into a test-tube labelled as A. Test-tube A was immersed into the water bath with a temperature of 15C.

Figure 1 shows the method used in the experiment:

Thermometer Iodine solution Water bath ( 15C) 1 ml saliva Figure 1 Step 4: 5ml of starch suspension was added into test tube A. The mixture was stirred and a drop of the mixture was taken out and dropped into the first groove of the tile containing iodine solution. The iodine test was repeated every minute for 1 minute. White tile

Figure 2 shows the result obtained at the end of the experiment: Blue black solution Time taken for the complete hydrolysis of starch ___ minute Brown iodine solution Figure 2 Steps 1 to 4 are repeated by using the different sets of test tubes labelled as B, C and D, and the test tubes are immersed in water baths with temperatures at 25C, 35C and 45C respectively.

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SBP PERFECT SCORE BIOLOGY SET 4 (P3)

Table 1 shows the observations of the drops on the white tile after each experiment: Temperature (C) Time taken for the complete hydrolysis of starch (min)

Test tube

Observation on the white tile

25

35

45

Table 1

1(a)(i) Record the time taken for the hydrolysis of starch to be completed at a temperature of 15C in the space provided in Figure 2. (ii) Record the time taken for the hydrolysis of starch to be completed at different temperatures in the boxes provided in Table 1. [3 marks] (b) (i) State two observations on time from Figure 2 and Table 1 1. .. .. 2 . .. .. [3 marks]

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SBP PERFECT SCORE BIOLOGY SET 4 (P3)

(ii) State two inferences from the observations in (b)(i). 1. .. .. 2 . .. .. [3 marks] (c) Complete Table 2 based on the experiment that was carried out: Variables 1. Manipulated variable Particulars to be implemented How to alter the manipulated variable . . ..

2. Responding variable

How to determine the responding variable .

..

3. Controlled variables

How to maintain the controlled variables .

..

[3 marks]

(d)

State the hypothesis for this experiment .. .. [3 marks]

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SBP PERFECT SCORE BIOLOGY SET 4 (P3)

(e)(i) Construct a table to record the results of this experiment. Your table should contain the following titles: Temperature Time taken for reaction to be completed Rate of reaction, 1/time Temperature (C) Time taken (min) 15 25 35 45 Plot a graph of rate of enzyme reaction against temperature based on the table : Rate of reaction, 1/time (min-1)

[3 marks] (e)(ii) Based on the graph in (e)(i), state the relationship between the rate of enzyme reaction with temperature of the medium .. .. [3 marks]
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SBP PERFECT SCORE BIOLOGY SET 4 (P3)

(f)

Based on the results of the experiment, what can you deduce about enzyme? .. .. [3 marks]

(g)

If another set of experiment was prepared and immersed into the water bath at a temperature of 70C, predict the time taken for the enzymes to hydrolyse the starch completely. Give a reason for your prediction. .. .. [3 marks]

(h)

Draw an arrow to match the enzyme with the substrate given in the boxes below: Amylase Peptidase Maltase Sucrase Pepsin Lipase Starch Sucrose Maltose Lipid Protein Peptide

[3 marks]

END OF QUESTION PAPER

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SBP PERFECT SCORE BIOLOGY SET 4 (P3) Question 2

Microorganisms are not always active. The growth and activities of microorganisms are influenced by various abiotic factors such as temperature, pH, light intensity and the presence or absence of nutrients.

Based on the above information, design a laboratory experiment to investigate the activity of yeast. Your experimental planning need to include the following aspects: Statement of identified problem Objective of study Variables Statement of hypothesis List of materials and apparatus Technique used Experimental procedures Presentation of data Conclusion

the effect of pH, on

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SBP PERFECT SCORE BIOLOGY SET 4 (P3) Question 3 A group of students carried out an experiment to study the effect of light intensity on the rate of photosynthesis Diagram 1(a)(i) shows the set up of the experiment. Diagram 1(a)(ii) shows the time taken for the experiment Diagram 1(b) shows the result of the experiment done at the distance of 20 cm,40 cm,60 cm and 80 cm from the bulb (menthol)

Boiling Tube Gas bubbles beaker Aquatic plants Water bath (370C) Sodium hydrogen carbonate solution

DIAGRAM 1(a)(i): SET UP OF THE EXPERIMENT Time taken for the experiment/min

Diagram 1 (a)(ii)

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SBP PERFECT SCORE BIOLOGY SET 4 (P3)

Diagram 1(b) : Result of the experiment

For Examiner s Use

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SBP PERFECT SCORE BIOLOGY SET 4 (P3) 1(a) (i) Based on diagram 1(b), state two observations that can be made from the experiment. Observation 1: . Observation 2: [3 marks] (ii) State the inference for each observation made in (a) (i). Inference for observation 1 ......................... Inference for observation 2 .................................. 1 (a) (ii) 1(a)(i)

[3 marks] (b)(i) (ii) Record the time taken for the experiment in the space provided in diagram (b)(i)(ii) 1(a)(ii). Record the number of gas bubbles released in the space provided in diagram1(b) at the distance of 20 cm,40 cm,60 cm and 80 cm in the time given. [3 marks]

(c)

Complete Table 1 based on the experiment that was carried out..

Variables Manipulated variable

Particulars to be implemented How to alter the manipulated variable 52

SBP PERFECT SCORE BIOLOGY SET 4 (P3) . Responding variable How to determine the responding variable Controlled variable How to maintain the controlled variable

1 (c)

(d)(i)

[3 marks] State the hypothesis for this experiment. .

1(d)(i)

. [3 marks] (ii) Record the result of the experiment in the table below. Plot a graph of rate of photosynthesis against distance. Distance (cm) 20 40 60 80 Number of gas bubbles released Rate of photosynthesis (Number of bubbles min -1)

1 (d)(ii)

[3 marks]

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SBP PERFECT SCORE BIOLOGY SET 4 (P3)

(d)(iii)

Explain the relationship between the rate of photosynthesis and the light intensity [3 marks]

1(d)(iii)

(e)

The experiment is repeated, but by increasing the temperature of the water bath to 600C. Predict the effect of increasing the water temperature to the rate of photosynthesis.

1 (e)

. [3 marks] (f) State the relationship between the increase of temperature and the rate of photosynthesis. 1 (f) 54

SBP PERFECT SCORE BIOLOGY SET 4 (P3) . [3 marks] (g) Based on the experiment, define photosynthesis operationally. . . [3 marks] (h) Another group of students had carried out the experiment to study the effect of carbon dioxide concentration on the rate of photosynthesis but using the following apparatus and materials. Hydrilla sp, sodium hydrogen carbonate powder, distilled water, light bulb. Categorised the apparatus and materials in their experiment. [3 marks] 1 (g)

1 (h)

Question 4

Plant needs macronutrients and micronutrients to grow healthy and reproduce. Nitrogen as in nitrate, phosphorus and potassium are most important. By using a suitable plant that can absorb nutrient from nutrient solution (aquaculture), design an experiment to show the effect of nitrogen, phosphorus and potassium deficiency on this plant. Your experimental planning need to include the following aspects: Statement of identified problem Objective of study Variables Statement of hypothesis List of materials and apparatus Technique used Experimental procedures Presentation of data Conclusion

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