Dr.

Rajesh Kumar
Ph.D (Dairy Microbiology)
Molecular Biology Unit
Dairy Microbiology Division
N.D.R.I
rajesh.mbu@gmail.com
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 BIOSAFTEY TRAINING
Good Laboratory Practice.
Emergency procedures
Biosecurity
Biological Safety/GMOs‘‘
Wastes
Chemical safety
Transport of biological samples
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 Good Laboratory Practice
Strict Definition:
“A quality system concerned with the organisational
process and the conditions under which non-clinical
health and environmental safety studies are planned,
performed, monitored, recorded, archived and reported.”
How does GLP relate to academic
research?
1. Safety
2. Reproducibility
3. Reporting
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1) Biosafety – your responsibilities

Those who work in the University/organization

should be aware of the University’s Statement of
biosafety Policy (http://www.dbtbiosafety.nic.in)

Everyone must know the risks and potential

hazards of the materials and equipment he/she is
working with and so be alert to any dangers.
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 You should also:
1. Have had a safety induction & training – check the
local rules in all laboratories that you work in
2. Know who your Safety Officer is – seek advice,
report any incidents
3. Consider your health status (e.g. allergies) & any
vaccination requirements (e.g. Hepatitis B)
4. Complete risk assessments so that you know
• how to use, store and dispose of all chemicals
• how to deal with any spills or accidents
• the safety precautions required and for some
equipment, e.g. safety cabinets, how they work
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 Risk Assessments
Hazardous Chemicals -
Control of Substances Hazardous to Health
Regulations 2002 (COSHH).
Biological Hazards –
Genetic manipulation, handling pathogens,
Crime & Security Act 2001
Radiochemicals –
Contact the safety office for permission
before use.
Experimental risk assessments –
Combination of hazards associated with a
technique or protocol.
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 Emergency Procedures
What to do in case of an accident or incident ?
Telephone numbers:

or

Experimental Hall Operators:

2525(24h/24h)
Safety Group :
2403
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 EMERGENCY PROCEDURES
The safety signs to recognize
Emergency exits and evacuation routes

Fire extinguishers

Spill kits and First aid kits

Emergency showers

Eye-wash fountains

Know your nearest first aiders

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 SAFETY ICONS Safety reminders

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 HAZARD ICONS Safety reminders

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 Hazard symbols
Radioactive Highly flammable

You must be able to recognise and explain the

significance of the following hazard symbols.

Oxidizer Harmful Biohazard Corrosive Toxic

Look for them around the laboratory – all your
chemicals should be labelled appropriately
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 Personal protective equipment

But also be aware of hazards

from your work possibly
affecting others working around
you – use a fume cupboard, warn
others, restrict access to where
you are working.
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 Safety cabinets
Class I biosafety
Protects the user from
aerosol hazards

Fume cupboards
Designed to exhaust fumes from
the laboratory environment.

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 Class III biosafety
User works through gloves. The
material within the cabinet is
protected by hepa-filtered air.
The inside of the cabinet
operates at a negative pressure.

Class II biosafety
Protects both the user and the
material within the cabinet
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 Biological hazard Categories
HAZARD GROUP 1- Agent is unlikely to cause human
disease
HAZARD GROUP 2- Agent can cause human disease and
may be a hazard; unlikely to spread to the community;
effective prophylaxis or effective treatment available.
HAZARD GROUP 3- Agent can cause severe human
disease and presents a serious hazard; may present a risk
of spreading to the community; effective prophylaxis or
treatment available.
HAZARD GROUP 4- Agent causes severe human disease
and is a serious hazard; likely to spread to the
community; no effective prophylaxis or treatment
available.
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All work with Group 3 pathogens
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 Blood and human tissue
Particular concern over the potential presence of infectious
agents including Hepatitis B & HIV.
It is recommended that any worker be successfully immunised
against HepB before commencing work with human/primate
samples.

Usual recommendations:
Check your risk assessment & local rules before starting,
Assess the HepB/HIV risk in samples,
Work under biocontainment conditions (safety cabinet),
Take care in disposal (e.g. double-bag & autoclave prior to disposal
with clinical waste)

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 Biological Safety
Biological agents
Any agent which may cause any infection, allergy, toxicity or can
create any hazard to human health and/or environment.
Micro-organisms such as
Fungi (including yeasts), Bacterias, Viruses, Prions,

Parasites
Genetically modified organisms (GMO’’s)
Cell cultures such as
Mammalian cell cultures (animal and human)
Plant cell cultures
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 Biological Safety
Hazard groups (Since 1994)

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 Some rules for classifying…
The classification does not take account of other
hazardous properties eg. toxic, oncogenic or allergenic
properties.

The categorisation is based on the infective hazard to

healthy adult worker, it does not allow for any ,
additional risk to individual workers caused by:-

pre-existing disease
the effects of medication
compromised immunity
pregnancy or breastfeeding
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Hazard Biological Agent Disease
group
1 E. coli K12, Yeast Baculovirus None
Expression CHO cells
2 Pseudomonas aeruginosa Urinary tract & respiratory system
infections, dermatitis and variety
of systemic infections
2 Adenovirus type 1-5, 7 Rhinitis, pharyngitis, conjunctivitis,
cough…
2 Adenovirus type 40, 41 Nausea, vomiting, diarrhea, malaise
2 Eppstein Barr Virus Nasopharyngeal carcinoma, Burkitt’s
lymphoma
2 Vaccinia Virus Eczema, vesicular and pustular lesion
3 HVB, HBC, HIV, Bacillus Hepatitis B, Hepatitis C, AIDS,
anthracis Anthrax/Blackleg
4 Marburg Virus Hemorrhageic fever
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 Genetically modified organisms
Genetic Modification is defined as the altering of the
genetic material of an organism by a way that does not
occur naturally by mating and/or natural recombination.

It is the policy of the University that all work involving

GMOs is carried out in such a way as to prevent undue
risk either to those carrying out the work, to those
who may otherwise be affected by the work, or to the
environment.

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 Genetically modified organisms
Risk assessments for new projects must be
completed and submitted to a GM Safety
Committee

• hazard identification
• estimation of the severity of the consequence
• determination of a provisional containment class
• environmental considerations
• estimation of likelihood of harm occurring
• can all hazards be properly controlled

Results in assignment of the final containment

and activity classification 1-3 (classes 2 & 3
notifiable to HSE)
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 Genetically modified organisms
•Work must be carried out in a suitably equipped
and designated containment laboratory
•Periodic reviews of risk assessments done
•Health surveillance may be required
•Records of exposure may be required
•Emergency plans are needed

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Before starting a project….
Involving GMO’s…
Every project should be registered in the existing license for
contained use of genetically modified organisms (CGG)
The project may also be registered and approved by the safety
officer

Involving Pathogens and human cell lines…

 Work in a special designed laboratory
 Project registered and approved
 Specific training in using the laboratory and handling cell lines or
pathogens
 Check on medical follow follow-up (vaccinations if possible or
necessary)
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 Waste disposal

Waste must be disposed of correctly:

autoclave bags or the yellow carton bin dedicated for
incineration
Stainless steel container
Sharp bin containers
Treatment of liquid waste with disinfectant

STAFF SHOULD NEVER BE ENDANGERED BY

DISPOSING OF WASTE INAPPROPRIATELY
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 Biohazardous waste must be rendered safe
before disposal

Clinical waste

Autoclave
Disinfection

Sharps
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 Non-infectious biological waste
Liquid biological waste containing GMO’s must be
inactived by autoclaving before it can be poured down into
a plastic container for chemical waste treatment.

(Gelyfication of liquids in the pipeline)

ATTENTION: Solutions containing SeMet, corrosives or any

other toxic compound must be inactivated for biological
contaminant first, and treated afterwards as chemical
waste.
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 Chemical risks
How do I protect myself and the environment ?

Protocol well known.

Chemicals and material safety data sheet and (MSDS) in
sample lab or on the lab web read.
Use of small quantities of chemicals; quantities consider safer
substitute protocols when possible.
Wear safety glasses, proper gloves, and a lab coat when
handling chemicals
Disposal of of chemical waste appropriate.
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 Storage of chemical products
No more chemicals than needed should be stored.

Always read the chemical's label (and MSDS).

Mark it with the date of receipt/opening before storage.

Never store a chemical with an obscured or missing label.

Define separate storage areas for highly toxic chemicals.

Never store liquid hazardous chemicals above eye level.

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