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Tetrahymena
Week 4 BIOL 4272: Cell and Developmental Biology Lab Department of Biology and Biochemistry University of Houston
WATER
WATER
Flip-flop ( once per month) with flippases and ATP >> msecs
Endocytosis
In endocytosis, the cell takes in macromolecules by forming vesicles from the plasma membrane Endocytosis is a reversal of exocytosis, involving different proteins There are three types of endocytosis:
Phagocytosis (cellular eating) Pinocytosis (cellular drinking) Receptor-mediated endocytosis
Endocytosis
In phagocytosis a cell engulfs a particle in a vacuole The vacuole fuses with a lysosome to digest the particle
Endocytosis
EXTRACELLULAR FLUID
CYTOPLASM
Pseudopodium
1 m
Pseudopodium of amoeba
phagocytosis
Tetrahymena
after ingesting ink
Endocytosis
In receptor-mediated endocytosis, binding of ligands to receptors triggers vesicle formation A ligand is any molecule that binds specifically to a receptor site of another molecule
RECEPTOR-MEDIATED ENDOCYTOSIS
Coat protein
A coated pit and a coated vesicle formed during receptormediated endocytosis (TEMs)
Endocytosis
In pinocytosis, molecules are taken up when extracellular fluid is gulped into tiny vesicles also called constitutive endocytosis
Constitutive Endocytosis
can be imaged with fluorescent vital stains aminostyryl pyridinium molecules
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FM 1-43
nonfluorescent in aqueous environment rapid resonance across ethylene bonds stabilized in nonpolar environments such as phospholipid bilayers
FM 1-43
FM 1-43
initially used to study events at the neuromuscular junction synaptic vesicle recycling many studies subsequently use for a variety of dynamic membrane events like endocytosis in mammals and amphibians (must read review paper on Bb) can be used in Tetrahymena