“ANTIBACTERIAL ACTIVITY OF

HOMOEOPATHIC DRUGS IN VITRO”

SIMILIBIOTICS
 DR.K.M.NISANTH NAMBISON
BHMS (Chg), M.F.Hom (Mal), FBIH (London), PGDCA, MDP (IIM)
(Gold Medalist)
Asst. Professor,
Department of Surgical Therapeutics,
Govt. Homoeopathic Medical College, Bhopal (M.P) INDIA
Professor,
Faculti of Homeopathy, Malaysia
 DR.SMITA NAMBISON
BHMS, M.D (Cosmetology), PGDNHE
(Gold Medalist)
Chairperson,
Nambison’s SoftroniX
www.nambison.com; www.diagnozit.com
Ex-Asst. Professor,
Department of Pharmaceuticals,
N.S.Homoeopathic Medical College, Bhopal (M.P) INDIA
President,
SAAHAS, Society of Advanced Homeopathic Sciences
GUI DANCE:
Prof . Dr. (Mrs .) Q. Khan
MBBS, MD (Microbiology)
       Head of Department of Microbiology,
Gandhi Medical College, Bhopal (MP), India
INS PI RA TIO N:
Prof . Dr. Bh upendra Shri va st av
MBBS, DA, DHMS
Direc tor ,
LBS Hospital Bhopal (MP), India

Prof . Dr. An and Kumar Chat urv edi

B.Sc. , BH MS, M .D (Ho m)
Principal,
Anushree Homoeopathic Medical College Jabalpur (MP), India
 SIMILIBIOTIC:
“Homoeopathic drug having action
similar to the pathogenic effect of
microorganism is a Similibiotic.”
INTRODUCTION: The conc ept and ev en the
at tem pt to use su bstanc es derive d from one
organis m to ki ll another organis ms
(anti bios is ) are alm ost as old as the sc ie nce
of bac ter iology .

(e.g. mouldy curd of Soy a bean applied to

boil s).
 Boil due to
Microorganism Mouldy Curd of
Soyabean, which
Apply
has
Microorganism
Eradication of
Infection

Disappearance of
Boil

ANTIBIOSIS
(Resembling Similia Similibus Currentur)
Thi s in one-way or the other res emble s
homoeopat hic law “ Si mi lia Si mi li bus
Curr ent er” i.e. l ik es are cured by l ik es .
The Scient is ts Pas te ur and Joubert 1877
confi rmed this , they not ed that ant hr ax
bac il li grew rapidl y when inocc ul ated into
steri le urine but fai led to mult ipl y and soon
died if one of the “ co mmon” bac ter ia of the
air was int roduc ed i n urine at t he s am e tim e.
Wit h the break th rough in vent ion of the
ant ibiot ic , Penic il li n, whic h is produce d by
Penic il lin Notanum a mould, sh own by
Alex ander Fl eming in 1928, deat h due to
inf ec ti ous di seas e drast ic al ly , came dow n.
Sinc e then newe r ant ibiot ic s cam e in to the
mark et and prove d to have exc el lent
ther apeut ic pot enti al in curing the
pat hogenic m anif es tat ions .
But am ids t all this , drug res is tanc e has
bec ome a m enac e for m edic al sci ence .
Now , hos t defenc e mechani sm is the mos t
im portant det er minant of ther apeutic
ef fec tiv enes s. Theref ore sti mulat ing o nes
im mune mec hanis m would be more
appropriate tool in co mbat ing the ra ising
inc idence of inf ec tious di seas es 1 , drug
res is tanc e and m utat ion.
Alt hough to kill bact eria is not our aim since
bac teri a ar e not caus e of di sease, but
dis eas e li es in person hi ms elf (i. e.
susc ept ibi li ty ) and bact eria is merel y a
seco ndary cause.
-K ent
In the wonderf ul scienc e of Hom oeopathy of
def ence mech anis m is done by minut enes s
of Hom oeopathi c dr ugs , as Arndt-Sh ultz law
Stat es that “ Large doses ki ll; Modera te dose
inhi bit ; and s mal l dos es sti mul ates ”.
 Hom eopathy
ai ds/s tim ul ates t he
natural abi lit y of our
body to heal i tsel f.
 CHEM ICA L NATURE OF HOMOEOPA THI C
DRU GS :-
Almos t al l hom oeopathi c drugs are
made through serial dil utio ns of Alc ohol i. e.
Ethanol (C 2 H 5 OH) (Mol. wt. 46.07) and strength
83. 1% w/w. 5
Alc ohol in st rengt h 60% to 90% w/ w
is know n for it s ant ibi oti c ac tiv it y. 1
POTENTISATION OF HOMOEOPATHIC DRUGS :-

Ethanol (C2H5OH) + Drug +

Strokes (Power)

Serial dilutions

Homoeopathic potency
retaining pharmacological
message although no
detectable molecule of drug is
present (above 24X or 12C).

Serial dilut ion reduc es quant ity of drug but i mmensely raises it s quality.
 MA TE RIA LS & M ETHO D: -
1.Collection of samples:- Samples of infected
discharges( urine , pus , bronchial lavage) were
collected from Hamidia Hospital ,Bhopal and subjected
to culture.

2.Recording of Symptoms:- Symptomatology of

some cases were taken down and recorded for some
available cases.
 MA TE RIA LS & M ETHO D: -
1.Collection of samples:- Samples of infected
discharges( urine , pus , bronchial lavage) were
collected from Hamidia Hospital ,Bhopal and subjected
to culture.

2.Recording of Symptoms:- Symptomatology of

some cases were taken down and recorded for some
available cases.
 3. Selection of bacteria: - Bacterial Strains which
were highly sensitive to allopathic drugs were selected
with the help of Culture & sensitivity.
Table-2
 TABL E NO. 2
S.N o Spec im en for
Or ganis m is olated
. cult ure

1. Uri ne E.C oli , Klebs iel la, Pr oteus.

2. Pus Kl ebsiel la, Staphy loco ccus

Coagulas e -v e
3. Bl ood Staphy lococ cus Coagul ase - ve

4. Br onchia l Lavage E.C oli .

5. C.S.F. E.C oli

 4. Homoeopathic drugs Selection:- Those
Homoeopathic drugs which are frequently used for
Urinary tract infection were selected and tabulated in
Table - 1 with their potency.Since the hospital where
this study was done had innumerable cases of UTI and
most bacteria were isolated from urine.
5. Potency Selection: - Lowest potency
available, highest potency and common potency
available were selected to evaluate their efficacy.
 TA BLE NO .1
HOMOEOPATHIC DRUGS USED & THEIR POTENCIES
S.No. Homoeopathic drugs Potency

1. Apis Mellifica θ, 30, 200, 1M, 10M, CM.

2. Arsenic Album θ, 30, 200, 1M, 10M, CM.
3. Capsicum θ, 30, 200, 1M, 10M, CM.
4. Cantharis θ, 30, 200, 1M, 10M, CM.
5. Lycopodium θ, 30, 200, 1M, 10M, CM.
6. Mercurius Solibiliris 30, 200, 1M, 10M, CM.
7. Medorrhinum 6X, 30, 200, 1M, 10M, CM.
8. Pulsatilla θ, 30, 200, 1M, 10M, CM.
 Apis Mellifica
Arsenic Album

Cantharis
Capsicum
Mercurius Solibiliris Lycopodium

Pulsatilla Medorrhinum
 6. Control :- Ethanol discs were prepared and
kept as control. Each disc estimated to have 0.02ml of
Ethanol (83.1%w/w).
7. Sensitivity Discs:- Discs of 5mm diameter of
Whatman filter paper no.41 of round shape were cut
and properly sterilized.4
8.Drug Concentration of Discs: - 0.02cc of
concentration per disc of drug was achieved.
ANALY SI S OF BA CTE RIA L S ENS ITIV IT Y TO
HO MO EOPATHI C DRU GS: -
1. Petri dish of sterile Mueller Hilton Agar (pH
7.4 ± 0.2) was prepared.
2. Gram Staining was performed.
3. Incubated broth culture at (30 ± 1oC or 37 ±
1oC) after obtaining turbility was standardised by
Barium Sulphate Standard.
 4. Inoculation:- Now MHA plate was inoculated
with the bacteria and was kept at room temperature for
5 to 10 minutes since confluent growth was desired.
5. Application of Homoeopathic Disc :- Homoeopathic
drugs sensitivity discs were taken with the help of
flamed forcep and carefully placed on surface of
medium and complete contact was made.
 6. Incubation :- Plates were incubated at 35 o- 37 oC
for 18 -20 hours.
7. Reading of growth inhibition zone :-
Measurement of outer margin of disc to the zone of
disc to the zone of inhibition was done in millimeters
with the help of measuring scale.
8. No. of cases = 15.
9. Microorganism :- E.Coli , Klebsiella , Proteus ,
Staphylococcus coagulase-ve.
 It is a wonder that although Alcohol in the strength
60% to 90% is known for its antibiotic activity1
Homoeopathic drugs in the present study have shown
bigger GIZ than alcohol, which were kept as controls.
This indicates that GIZ of Homoeopathic drug is not
due to the effect of ethanol,
ethanol but some other dynamic
pharmacological message seems to be behind the
formation of growth inhibition zone.
 RES ULT: Present study shows biggest growth
inhibition zone of 3mm., which is considered to be
resistent according to International standard, but in
clinical practice it is generally considered to be weakly
sensitive. Biggest growth inhibition shown by the
medicine is given in Tabl e No. -3
 TABLE-3 Biggest Zone of Inhibition in (mm)
Potency Q 30 200 1M 10M CM
S.No. Medicine

1. Apis 1mm 2mm 2mm 1mm 2mm 2mm

2. Ars. Alb. 2mm 2mm 2mm 2mm 1mm 2mm
3. Capsicum - 3mm 2mm - 2mm -
4. Cantharis 2mm 2mm 1mm 2mm 2mm 2mm
5. Lycopodium 2mm - 2mm 2mm - 3mm
6. Merc.Sol. - 1mm 3mm 1mm 2mm 2mm
7. Medorrhinum (6X) 2mm 1mm - - 3mm 2mm
8. Pulsatilla 1mm 3mm - 3mm - 3mm

Ehanol showed GIZ of 1mm .

 GIF in mm

0
0.5
1
1.5
2
2.5
3
Apis
Ars.Alb
Caps
Canth
Lyco

Medicine
Merc.S.
Medo
Pulsatilla
0
30
200
1M
10M
Graphical Presentation of the Result

CM
Po
ten
cy
0
30

1M

CM
200

10M
 Plate No. 1
Petri dish of sterile Mueller Hilton Agar with bacterial culture showing GIF
around Homoeopathic discs.
 Plate No. 1
Petri dish of sterile Mueller Hilton Agar with bacterial culture showing GIF
around Homoeopathic discs.
 Corr ela ti on of res ult with pre sen ta tio n of sy mp tom s
by th e p ati ent :-
Presentation of symptoms due to infection were ready
taken down and redline symptom was the basis of selection of
remedy. Now bacteria were subjected to culture to see
sensitivity to homoeopathic drugs, to our surp rise the
medicine which was selected on the basis of redline symptoms
was simultaneously effective on the bacteria in vitro in the form
of growth inhibition zone around a particular medicine (which
was selected on the basis of similimum) in particular potency.
 This makes us conclude that when totality of drug
Pathogenesis is similar to pathogenesis of bacterial
activity and when this drug is inoculated on bacteria in
vitro, due to its similarity in antigenic property, drug
inhibits the growth of bacteria. (Similia similibus
curentur)
 Concl us ion: It is a wonder that Homoeopathic
dilution of Ethanol or distilled water where in probability
of finding even a single molecule of original drug is nil
(after 24X or 12C) it is potent enough to inhibit growth
of bacteria.
 Overall review makes us conclude that Homoeopathic
drugs used in infections are not antibiotics but are
Similbiotics (similar to bacteria) i.e. we administer the
patient, medicine which is capable of producing similar
symptoms in patient, as produced by bacteria hence
stimulating defence mechanism of host which kills the
bacteria.
 This is a unrevealed area of research where in more
research needs to follow which would promise a new
hope in cases where bacteria have become resistant to
each every known antibiotic. Rampant unindicated,
inappropriate and uncalculated uses of Antibiotics are
doing greater harms than any good to the human and
vets.
 SIM IL BI OTI CS (Hom oeopathi c)
can play an ins trument al role by not mak ing
the bact erial stai n re sistant , at the same
time ens urin g no mut at ion, thus pr ev ent ing
the s oc iet y from hazardous new forms of infection.
 ACCEPT WHAT IS ACCEPTABLE TO U
BUT, DO NOT REJECT WHAT IS NOT ACCEPTABLE
DIP YOURSELF IN THE TEA