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Descriptions
Innova Plex Taq DNA Polymerase is suitable for routine PCR. It is a thermostable enzyme that can withstand prolonged incubation at temperature up to 95C without signicant loss of activity. It is a recombinant type puried from the cloned Thermus aquaticus DNA polymerase gene expressed in E.coli. This enzyme is highly puried for its maximum activity and free of bacterial DNA through a unique purication system. For comprehensive optimization of PCR conditions 5X Band FinderTM solution is also included.
Benefits
High quality, High purity, and low price Easy and simple PCR optimization with Band FinderTM can help easy optimization to amplify various kinds of target DNA. Band FinderTM can resolve the PCR problems of high GC contents or complicated structures of primers and target DNA. The included reaction buer is specially designed for high-yield PCR production. A high quality of dNTP mixture is included for best result of your works
Quality
High Activity
M 1 2 3 4 5 6 7
M: 1kb Plus Ladder(Invitrogen) 1. 5 units 2. 2.5 units 3. 1.25 units 4. 0.625 units 5. 0.313 units 6. 0.156 units 7. 0.078 units M: 1kb Plus Ladder(Invitrogen) 1.2.5 U 2.1.25 U 3.0.625 U 4.0.313 U
Innova Plex
Competitor
Innova Plex
Competitor
Applications
High Purity To examine contamination of E.coli genomic and plasmid DNA in the process of Taq DNA polymerase purication, template free PCR was performed by using Taq DNA polymerase gene primer.
30 cycles 35 cycles
M: 1kb Plus Ladder(Invitrogen) 1. Innova Plex 2. Competitor T 3. Competitor R 4. Competitor S
1
INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Product Name
Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.4ml) Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 2.0ml) Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 4.0ml)
Units
500U (500*5/pkg) 2500U (500*10/pkg) 5000U
2
INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Descriptions
Innova Plex fx-Taq Polymerase is a modied enzyme of Innova Plex Taq polymerase and suitable for routine PCR, MS Genotyping, SNP, and Multiplex-PCR etc. The modication enhances the amplication specicity and minimizes the dimmer formation and non-specic PCR products. Unlike other chemically modied PCR enzymes, fx-Taq polymerase shows high productivity as well as the high specicity. With this innovative enzyme the problematic PCRs due to high GC or structural problems of target DNA or primers could be easily solved. This enzyme is highly puried for its maximum activity and free of bacterial DNA through a unique purication system. For easy and stable PCR reactions high-quality dNTP mixture is included. For comprehensive optimization of PCR conditions 5X Band Finder solution is also included.
Benefits
High quality, hgh purify, and low price. Easy and simple PCR optimization with Band Finder can help easy optimization to amplify various kinds of target DNA. Band Finder can resolve the PCR problems of high GC contents or complicated structures of primers and target DNA. Clean results by minimized primer dimer and non-specic band. The included reaction buer is specially designed for high-yield PCR production. A high quality of dNTP mixture is included for best result of your works.
Contents
fx-Taq DNA Polymerase 5U/ul 10x fx-Taq reaction buer 10mM dNTP Mix (each 10mM) 5x Band Finder
Storage Buffer
50% 20mM 100mM 0.1mM 1mM 0.5% 0.5% Glycerol Tris-HCl (pH8.0) KCl EDTA DTT Tween 20 Nonidet P-40
Products
Catalog No.
IFT06-R500 IFT06-R25h
Product Name
fx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.4ml) fx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 2.0ml)
Units
500U (500*5/pkg) 2500U
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Descriptions
Innova Plex hx-Taq polymerase is a modied enzyme of Innova Plex Taq polymerase and suitable to use for hot start PCR, MS genotyping, SNP, Multiplex-PCR, and real-time PCR etc. The chemical modication makes the enzyme inactive at low temperature and dramatically reduces the non-specic amplications during the PCR. This enhances the amplication specicity and minimizes the dimmer formation and non-specic PCR products. With this innovative enzyme the problematic PCRs due to high GC or structural problems of target DNA or primers could be easily solved. This enzyme is highly puried for its maximum activity and free of bacterial DNA through a unique purication system. For easy and stable PCR reactions high-quality dNTP mixture is included. For comprehensive optimization of PCR conditions 5X Band Finder solution is also included.
Benefits
High quality, hgh purify, and low price. Easy and simple PCR optimization with Band Finder can help easy optimization to amplify various kinds of target DNA. Band Finder can resolve the PCR problems of high GC contents or complicated structures of primers and target DNA. The included reaction buer is specially designed for high-yield PCR production. A high quality of dNTP mixture is included for best result of your works.
Contents
hx-Taq DNA Polymerase 2.5U/ul 10x hx-Taq reaction buer 10mM dNTP Mix (each 10mM) 5x Band Finder
Storage Buffer
50% 20mM 100mM 0.1mM 1mM 0.5% 0.5% Glycerol Tris-HCl (pH8.0) KCl EDTA DTT Tween 20 Nonidet P-40
Products
Catalog No.
IHT06-R250 IHT06-R10h
Product Name
hx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.2ml) hx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.8ml)
Units
250U (250U*4/pkg) 1000U
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Descriptions
Innova Plex EFx-Taq DNA Polymeraseis ideal for long(up to 40kb) and accurate PCR. It has higher delity, productivity and processing ability than Taq polymerase. It is a kind of blend product, a mixture of Taq and pfu DNA polymerase. Several special cofactors added to the enzyme mixture enhance the productivity of long PCR. This enzyme is highly puried for its maximum activity and free of bacterial DNA through a unique purication system. For easy and stable PCR reactions high-quality dNTP mixture is included. For comprehensive optimization of PCR conditions 5X Band FinderTM solution is also included.
Benefits
High quality, hgh purify, and low price. Easy and simple PCR optimization with Band Finder can help easy optimization to amplify various kinds of target DNA. Band Finder can resolve the PCR problems of high GC contents or complicated structures of primers and target DNA. The included reaction buer is specially designed for high-yield PCR production. A high quality of dNTP mixture is included for best result of your works. (The quality of dNTP mixture is one of the most critical things for long-PCR)
Quality
High Activity 1. PCR amplication with EFx-taq DNA Polymerase
M1 1 2 3 4 M2
- Template: Human Genomin DNA - Target size: 14, 17, 20, 22kb - Amplicaiton cycle: 35cycles - Reaciton volume: 50ul 2. Activity of EFx-taq DNA Polymerase
M 1 2 3 4 5 6
M1: 1kb Plus Ladder(Invitrogen) M2: Lambda/Hind III 1. 14 kb 2. 17kb 3. 20kb 4. 22kb
- Template: Human Genomin DNA - Target size: 17kb - Amplicaiton cycle: 30cycles - Reaciton volume: 50ul
M1: 1kb Plus Ladder(Invitrogen) 1.EFx-Taq(2.5U) 2.EFx-Taq(1.25U) 3.Competitor A(Product A:2.5U) 4.Competitor A(Product A:1.25U) 5.Competitor A(Product A:2.5U) 6.Competitor A(Product A:1.25U)
High Purity To examine contamination of E.coli genomic and plasmid DNA in the process of Taq DNA polymerase purication, template free PCR was performed by using Taq DNA polymerase gene primer.
Products
Catalog No.
IEF16-R250
Product Name
EFx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.3ml)
Units
250U
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Descriptions
Innova Plex pfu-X DNA polymerase is suitable for high-speed, high delity and long size PCR. It is applicable to high-delity PCR, such as the cloning experiments for protein expressions. The PCR product amplide by this enzyme have blunt-ends. Innova Plex pfu-X DNA polymerase has much higher activity and processing ability more than other brands of high-delity thermosatable enzymes.
Benefits
High quality, high delity, high processivity, long range detection and short run time and low price. Because of blunt-ended PCR product, TA cloning is not available. For TA cloning of PCR product amplied by this enzyme, PCR product is required to extend by Taq DNA polymerase. Easy and simply PCR optimization with Band Finder can help easy optimization to amplify various kinds of target DNA. Band Finder can resolve the PCR problems of high G+C contents or complicated structures of primers and target DNA. The included reaction buer is specially designed for high-yield PCR production.
Contents
pfu-X DNA Polymerase 2.5U/ul 10x pfu-X Buer 10mM dNTP Mix (each 10mM) 5X Band Finder
Storage Buffer
50% 50mM 100mM 0.1mM 1mM 0.1% 0.1% 1mM Glycerol Tris-HCl (pH8.0) KCl EDTA DTT Tween 20 Nonidet P-40 PMSF
Products
Catalog No.
IPX 16-R250 IPX 16-R500
Product Name
pfu-X DNA Polymerase with 10mM dNTP MIX(each 10mM, 0.3ml) pfu-X DNA Polymerase with 10mM dNTP MIX(each 10mM, 0.6ml)
Units
250 U 500 U
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Descriptions
Innova Plex Mini-prep kit is designed of rapid isolation of high-quality & high-quantity plasmid. The principle of plasmid purication of this kit is based on the alkaline-lysis method and the binding properties of DNA on the silica-gel membrane. The high-quality plasmids isolated from this kit is adequate for routine molecular biology application such as automated sequencing, cloning , PCR, in vitro transcription, transfection experiment etc. is also specially designed for high yield isolation of low-copy plasmid and much large size of circular DNA such as Cosmid or BAC clones. Spin column type Binding capacity: 20ug DNA/column Recovery yield: above 80%
Quality
Comparative quality test
Supplier A Innova Plex Supplier B High-copy number plasmid Low-copy number plasmid Large size of plamid
160
Innova Plex Supplier A Supplier B High-copy Low-copy number number plasmid plasmid Large size of plamid
Products
Catalog No.
IPM01-C100 IPM01-C200
Product Name
Plasmid Mini-prep Kit Plasmid Mini-prep Kit
Units
100preps 200preps
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Innova Plex PCR purication kit is designed for rapid and high-yield cleanup DNA from PCR and other enzymatic reaction mixture. The high purity DNA isolated using this kit is adequate for any molecular biology applications. Especially, the introduced unique system (DR condition) for the ecient removal of primer dimers, by-products of PCR, insures excellent results of automated sequencing and cloning. Spin column type DNA size: 70bp~20kb Recovery yield: above 80% Ecient removal of primer dimer
Benefits
One-step DNA clean-up from PCR or other enzymatic reactions Applicable to broad range of size(from 70 bp to 14kb) Two conditions are available for best results. Under the DR(dimer removal) condition, small fragment (less than 100bp) of DNA could be completely removed and you can obtain best results of automatic sequencing or cloning works. Under the HY(high yield) condition, all of the applicable size of DNA (from 70bp to 14kb)could be obtained with above 80% yield. High reproducibility and stability
Quality
Comparative quality test
Supplier A
O B A
Innova Plex
O B A Recovery Yield
1.2 1.0
Condition A: Innova Plex HY condition.2 volumes of isopropanol and 2 volumes of each binding buer were applied to 1 volume of original PCR mixture. Condition B: Innova Plex DR conditions.5 volumes of binding buers were applied to 1 volume of original PCR mixture.
M: size maker
Products
Catalog No.
IPP02-C100 IPP02-C200
Product Name
PCR Purication Kit PCR Purication Kit
Units
100preps 200preps
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Gel Extraction kit is designed for high-yield &high-quality isolation of any type of DNA(linear or circular size of 50 bp-20kb)from an agarose gel after gel electrophoresis. This kit is adequate for preparation of high quality DNA for automated sequencing , cloning, uorescent or radioactive labelling, in vitro transcription and other molecular biology applications. Spin column type DNA size:50bp~20kb recovery yield:above 80%(70bp~10kb) [Fragments of shorer than 700 bp or longer than 10kb show 50~70% yield]
Benefits
Easy and safe purication of DNA from agarose gels Preparation of highly puried DNA with highest yield Applicable to broad range of size( from 70 bp to 14kb) High reproducibility and stability
Quality
Comparative quality test
Supplier A Innova Plex Supplier B 90bp 200bp 300bp 500bp 700bp 1.2kb 1.5kb 4.0kb 14.0kb
1.2
Recovery Yield
Products
Catalog No.
IGE03-C100 IGE03-C200
Product Name
Gel Extraction Kit Gel Extraction Kit
Units
100preps 200preps
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Innova Plex Gel & PCR purication System Kit is designed to extract fragments of 50bp~20kb from an agarose gel after gel electrophoresis or to purify PCR products directly from a PCR reaction mixtures. The introduced unique system (DR condition) is highly ecient to remove the primer dimers, by-products of PCR . This kit can be used for preparation of high quality DNA for automated sequencing, cloning, uorescent or radioactive labeling, in vitro transcription and other molecular biology applications. Spin column type DNA Size : 50bp-20kb Recovery Yield : above 80%(70bp-10kb)
Benefits
Applicable to gel extraction and PCR purication by one kit High yield and high purity DNA purication High quality purication of DNA from 70bp to over 14kb
Compostion
UB : Universal buer WB : Washing buer(80% EtOH) Only empty bottle is provided because Ethanol is easy to volatilize or deteriorate. EB : Elution buer Spin column
Quality
PCR Purication
M C A-1 B-1 A-2 B-2 M
Gel Extraction
M C Q SG SU M C Q SG SM
glo-F-R1
M C Q
SG
SU
M C Q
SG
SM
glo-F-R3
glo-F-R5
PCR Purication M: 1kb DNA Ladder A-1: PCRB Dimer Removal B-1: Gel & PCR Dimer Removal A-2: PCRB HighYield B-2: Gel & PCR High Yield C: Control(original sample)
Gel Extraction M: 1kb DNA Ladder C: Control(original sample) Q: Q company sample SG : GEB prep SU: Gel & PCR prep
Products
Catalog No.
IGP04-C100 IGP04-C200
Product Name
Gel & PCR Extraction System Gel & PCR Extraction System
Units
100preps 200preps
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Descriptions
Sequence Saver SolutionTM is a noble reagent for economic sequencing reactions of plasmids or PCR products. A good sequencing results can be obtained with extremely small amount of BigDyeTM (0.1250.25ul) or DYEnamicTM ET Terminator (0.25-0.5 ul) ready reaction mix by adding Sequence Saver SolutionTM into the reactions. With Sequence Saver SolutionTM the sequencing cost could be greatly reduced without compromising data quality. Sequence Saver SolutionTM is fully applicable In the BigDyeTM or DYEnamicTM ET Terminator sequencing reactions and suitable for analysis using automatic sequencers.
Benefits
Saving cost for sequencing of plasmids or PCR products. Reliable and stable results without compromising data quality Easy adaptation to your bulk-sequencing reaction system.
Quality
Plasmid(pGEM vector) sequencing
Template: Plasmid Template: PCR product
Big dye 0.125 ul Big dye 0.25 ul Big dye 1.0 ul Fig 1. Sequence Save Solution Eect of DNA Sequencing Sequence Saver SolutionTM Eect of DNA Sequencing Template: plasmid sequencing (150-200ng/ul) PCR product sequencing (10-20ng/ul) 1:3 Diluted BigDyeTM Mix (Big Dye: Dilution Buer = 1:3) Sequence Saver SolutionTM Amplication cycle: 30cycles Reaction volume : 10ul
Products
Catalog No.
ISS31-B800 ISS31-B32h ISS31-B16k
Product Name
SEQUENCE SAVER SolutionTM /800rxns Sequence Saver SolutionTM /3,200rxns Sequence Saver SolutionTM /16,000rxns
Units
1.6ml 6.4ml 32ml
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Compositions
2X Real Time PCR premix 1ml*4 20X Innogreen 500ul*1
Quality
Sensitive Quanticaiton of Target Gene
HBV strong positive control DNA serial dilution (an undiluted solution(1), 1/5, 1/10, 1/100 - standard) NTC = Negative control (No template).
(User Data) qPCR for cDNA form cold-treated Arabidopsis (Targeted to a cold -responsive gene) E** - Green dye
A1 and B1, control(untreated) A2 and B2, 3hours cold-treated A3 and B3, 24hours cold-treated
Products
Catalog No.
IRH 71-M40h
Product Name
2X Real-Time PCR Premix (25mM MgCl2), Innogreen 500ul added
Units
4ml
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Quality
Innova Plex Supplier A Supplier B
1. Total RNA 50ng 2. Total RNA 25ng 3. Total RNA 12.5ng 4. Total RNA 6.5ng 5. Total RNA 3.2ng 6. Negative control (without RNA)
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
Products
Catalog No.
IOR51 E20h
Product Name
Reverse transcriptase(RNase H-)
Units
10000 U
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
M-MLV Rtase(RNase-)
Descriptions
Innova Plex Reverse Transcriptase is a recombinant enzyme puried from an E.coli strain harboring a plasmid with a part of pol gene encoding Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase. The enzyme exhibits an RNA-directed DNA polymerase activity but lacks ribonuclease H activity. Innova Plex Reverse Transcriptase also contains a special thermo-stable chaperon which makes the reverse transcriptase more stable and active at high temperature.
Quality
Innova Plex Supplier A Supplier B
1. Total RNA 50ng 2. Total RNA 25ng 3. Total RNA 12.5ng 4. Total RNA 6.5ng 5. Total RNA 3.2ng 6. D.W
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6
Products
Catalog No.
IMR 01 R10k
Product Name
M-MLV Rtase (RNase H-)
Units
10000 U
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Advantages
Fast & high yield No phenol, no chloroform High quality, less fragmentation Low cost & highest services Isolation of gDNA from various samples by one kit
Quality
Animal Tissue - 50mg Muscle Tissue A Q
M
B I Q I
Q
2 3 1
I
2 3
Q : Company Q Genomic DNA Prep Kit I : Innova Plex A : Pig muscle B : Hanow muscle
Q.C test
A B C M
1
INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Company Q Kit Q
A B C
I
D
Bacteria
M Q I
aroK (1.7kb)
Q I
Wild type E.coli : 500ul(1X108) Template : E.coli Genomic DNA Primer : aroE F/R, aroK F/R
Yeast
M A B C D E F M
Genomic DNA separation result from Yeast(2X107 cell/ml) using Innova Genomic DNA Prep kit(2X107) A, D : 0.5ml cel + lyticase(2.5u) B, E : 1ml cel + lyticase(2.5u) C, F : 2ml cel + lyticase(2.5u)
Products
Catalog No.
IGD41-C050 IGD41-C100
Product Name
Genomic DNA Prep Kit(blood, bacteria, plants, animal tissue ) - column type Genomic DNA Prep Kit(blood, bacteria, plants, animal tissue ) - column type
Units
50preps 100preps
2
INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com
Benefits
Superior LOD, eecive in amplicaiton of low copy DNA High activity, High sensitivity Even more eective than Taq DNA Polymerase in multiplex PCR condition setup.
*LOD(Limit of Detection) : Method to measure amount of the minimum sample concentration needed in PCR.
Quality
Sensitivitiy Test
M A B C D E F G M M A B C D E F G M
M : 1kb* DNA Ladder A : Template 10ng B : Template 2ng C : Template 0.4ng D : Template 0.08ng E : Template 0.016ng F : Template 0.008ng G : Negative (without Template)
Activity Test
M A B C D E F M
Company G
Template : Bovine Genomic DNA M : 1kb* DNA Ladder A : Enzyme 2.5unit B : Enzyme 1.25unit C : Enzyme 0.625unit D : Enzyme 0.3125unit E : Enzyme 0.15625unit F : Negative (without Enzyme)
Hs-Taq
Products
Catalog No.
IDT16-R500 IDT16-R25h IDT16-R50h
Product Name
AceStarTM Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 0.4ml) AceStarTM Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 2.0ml) AceStarTM Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 4.0ml)
Units
500U 2,500U 5,000U
INNOVAPLEX
Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com