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Taq DNA Polymerase

Descriptions

for Routine PCR

Innova Plex Taq DNA Polymerase is suitable for routine PCR. It is a thermostable enzyme that can withstand prolonged incubation at temperature up to 95C without signicant loss of activity. It is a recombinant type puried from the cloned Thermus aquaticus DNA polymerase gene expressed in E.coli. This enzyme is highly puried for its maximum activity and free of bacterial DNA through a unique purication system. For comprehensive optimization of PCR conditions 5X Band FinderTM solution is also included.

Benefits
High quality, High purity, and low price Easy and simple PCR optimization with Band FinderTM can help easy optimization to amplify various kinds of target DNA. Band FinderTM can resolve the PCR problems of high GC contents or complicated structures of primers and target DNA. The included reaction buer is specially designed for high-yield PCR production. A high quality of dNTP mixture is included for best result of your works

Quality
High Activity
M 1 2 3 4 5 6 7
M: 1kb Plus Ladder(Invitrogen) 1. 5 units 2. 2.5 units 3. 1.25 units 4. 0.625 units 5. 0.313 units 6. 0.156 units 7. 0.078 units M: 1kb Plus Ladder(Invitrogen) 1.2.5 U 2.1.25 U 3.0.625 U 4.0.313 U

1. PCR amplication with Innova Plex Taq


- Template: Human Genomic DNA(100ng) - Target size: 1.9kb - Amplication cycle: 35cycles - Reaction volume:50ul

Innova Plex

Competitor

2. Activity of Taq DNA Polymerase


Using original enqyme & buer - Template: Human Genomic DNA(100ng) - Target size: 1.9kb - Amplication cycle: 30cycles - Reaction volume:50ul

Innova Plex

Competitor

M: 1kb Plus Ladder(Invitrogen) 1.2.5 U 2.1.25 U 3.0.625 U 4.0.313 U

3. Activity of Reaction Buer


Using original enzyme & Innova Plex buer - Template: Human Genomic DNA(100ng) - Target size: 1.9kb - Amplication cycle: 30cycles - Reaction volume:50ul

Applications
High Purity To examine contamination of E.coli genomic and plasmid DNA in the process of Taq DNA polymerase purication, template free PCR was performed by using Taq DNA polymerase gene primer.
30 cycles 35 cycles
M: 1kb Plus Ladder(Invitrogen) 1. Innova Plex 2. Competitor T 3. Competitor R 4. Competitor S

Test for Plasmid DNA Contamination


- Target gene: Taq DNA Polymerase - Target size: 0.7kb - Amplication cycle: 30 or 35 cycles - Reaction volume:50ul

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INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

Taq DNA Polymerase


Products
Catalog No.
ITD16-R500 ITD16-R25h ITD16-R50h

for Routine PCR

Product Name
Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.4ml) Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 2.0ml) Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 4.0ml)

Units
500U (500*5/pkg) 2500U (500*10/pkg) 5000U

2
INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

fx-Taq DNA Polymerase


for High Specificity, High Productivity

Descriptions
Innova Plex fx-Taq Polymerase is a modied enzyme of Innova Plex Taq polymerase and suitable for routine PCR, MS Genotyping, SNP, and Multiplex-PCR etc. The modication enhances the amplication specicity and minimizes the dimmer formation and non-specic PCR products. Unlike other chemically modied PCR enzymes, fx-Taq polymerase shows high productivity as well as the high specicity. With this innovative enzyme the problematic PCRs due to high GC or structural problems of target DNA or primers could be easily solved. This enzyme is highly puried for its maximum activity and free of bacterial DNA through a unique purication system. For easy and stable PCR reactions high-quality dNTP mixture is included. For comprehensive optimization of PCR conditions 5X Band Finder solution is also included.

Benefits
High quality, hgh purify, and low price. Easy and simple PCR optimization with Band Finder can help easy optimization to amplify various kinds of target DNA. Band Finder can resolve the PCR problems of high GC contents or complicated structures of primers and target DNA. Clean results by minimized primer dimer and non-specic band. The included reaction buer is specially designed for high-yield PCR production. A high quality of dNTP mixture is included for best result of your works.

Contents
fx-Taq DNA Polymerase 5U/ul 10x fx-Taq reaction buer 10mM dNTP Mix (each 10mM) 5x Band Finder

Storage Buffer
50% 20mM 100mM 0.1mM 1mM 0.5% 0.5% Glycerol Tris-HCl (pH8.0) KCl EDTA DTT Tween 20 Nonidet P-40

Products
Catalog No.
IFT06-R500 IFT06-R25h

Product Name
fx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.4ml) fx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 2.0ml)

Units
500U (500*5/pkg) 2500U

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

hx-Taq DNA Polymerase


for High Specificity PCR

Descriptions
Innova Plex hx-Taq polymerase is a modied enzyme of Innova Plex Taq polymerase and suitable to use for hot start PCR, MS genotyping, SNP, Multiplex-PCR, and real-time PCR etc. The chemical modication makes the enzyme inactive at low temperature and dramatically reduces the non-specic amplications during the PCR. This enhances the amplication specicity and minimizes the dimmer formation and non-specic PCR products. With this innovative enzyme the problematic PCRs due to high GC or structural problems of target DNA or primers could be easily solved. This enzyme is highly puried for its maximum activity and free of bacterial DNA through a unique purication system. For easy and stable PCR reactions high-quality dNTP mixture is included. For comprehensive optimization of PCR conditions 5X Band Finder solution is also included.

Benefits
High quality, hgh purify, and low price. Easy and simple PCR optimization with Band Finder can help easy optimization to amplify various kinds of target DNA. Band Finder can resolve the PCR problems of high GC contents or complicated structures of primers and target DNA. The included reaction buer is specially designed for high-yield PCR production. A high quality of dNTP mixture is included for best result of your works.

Contents
hx-Taq DNA Polymerase 2.5U/ul 10x hx-Taq reaction buer 10mM dNTP Mix (each 10mM) 5x Band Finder

Storage Buffer
50% 20mM 100mM 0.1mM 1mM 0.5% 0.5% Glycerol Tris-HCl (pH8.0) KCl EDTA DTT Tween 20 Nonidet P-40

Products
Catalog No.
IHT06-R250 IHT06-R10h

Product Name
hx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.2ml) hx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.8ml)

Units
250U (250U*4/pkg) 1000U

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

EFx-Taq DNA Polymerase


for Long-range PCR

Descriptions
Innova Plex EFx-Taq DNA Polymeraseis ideal for long(up to 40kb) and accurate PCR. It has higher delity, productivity and processing ability than Taq polymerase. It is a kind of blend product, a mixture of Taq and pfu DNA polymerase. Several special cofactors added to the enzyme mixture enhance the productivity of long PCR. This enzyme is highly puried for its maximum activity and free of bacterial DNA through a unique purication system. For easy and stable PCR reactions high-quality dNTP mixture is included. For comprehensive optimization of PCR conditions 5X Band FinderTM solution is also included.

Benefits
High quality, hgh purify, and low price. Easy and simple PCR optimization with Band Finder can help easy optimization to amplify various kinds of target DNA. Band Finder can resolve the PCR problems of high GC contents or complicated structures of primers and target DNA. The included reaction buer is specially designed for high-yield PCR production. A high quality of dNTP mixture is included for best result of your works. (The quality of dNTP mixture is one of the most critical things for long-PCR)

Quality
High Activity 1. PCR amplication with EFx-taq DNA Polymerase
M1 1 2 3 4 M2

- Template: Human Genomin DNA - Target size: 14, 17, 20, 22kb - Amplicaiton cycle: 35cycles - Reaciton volume: 50ul 2. Activity of EFx-taq DNA Polymerase
M 1 2 3 4 5 6

M1: 1kb Plus Ladder(Invitrogen) M2: Lambda/Hind III 1. 14 kb 2. 17kb 3. 20kb 4. 22kb

- Template: Human Genomin DNA - Target size: 17kb - Amplicaiton cycle: 30cycles - Reaciton volume: 50ul

M1: 1kb Plus Ladder(Invitrogen) 1.EFx-Taq(2.5U) 2.EFx-Taq(1.25U) 3.Competitor A(Product A:2.5U) 4.Competitor A(Product A:1.25U) 5.Competitor A(Product A:2.5U) 6.Competitor A(Product A:1.25U)

High Purity To examine contamination of E.coli genomic and plasmid DNA in the process of Taq DNA polymerase purication, template free PCR was performed by using Taq DNA polymerase gene primer.

Products
Catalog No.
IEF16-R250

Product Name
EFx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.3ml)

Units
250U

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

pfu-X Taq DNA Polymerase


for High Processivity & Fidelity PCR

Descriptions
Innova Plex pfu-X DNA polymerase is suitable for high-speed, high delity and long size PCR. It is applicable to high-delity PCR, such as the cloning experiments for protein expressions. The PCR product amplide by this enzyme have blunt-ends. Innova Plex pfu-X DNA polymerase has much higher activity and processing ability more than other brands of high-delity thermosatable enzymes.

Benefits
High quality, high delity, high processivity, long range detection and short run time and low price. Because of blunt-ended PCR product, TA cloning is not available. For TA cloning of PCR product amplied by this enzyme, PCR product is required to extend by Taq DNA polymerase. Easy and simply PCR optimization with Band Finder can help easy optimization to amplify various kinds of target DNA. Band Finder can resolve the PCR problems of high G+C contents or complicated structures of primers and target DNA. The included reaction buer is specially designed for high-yield PCR production.

Contents
pfu-X DNA Polymerase 2.5U/ul 10x pfu-X Buer 10mM dNTP Mix (each 10mM) 5X Band Finder

Storage Buffer
50% 50mM 100mM 0.1mM 1mM 0.1% 0.1% 1mM Glycerol Tris-HCl (pH8.0) KCl EDTA DTT Tween 20 Nonidet P-40 PMSF

Products
Catalog No.
IPX 16-R250 IPX 16-R500

Product Name
pfu-X DNA Polymerase with 10mM dNTP MIX(each 10mM, 0.3ml) pfu-X DNA Polymerase with 10mM dNTP MIX(each 10mM, 0.6ml)

Units
250 U 500 U

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

Plasmid Mini-prep Kit


for rapid isolation of high-quality plasmid

Descriptions
Innova Plex Mini-prep kit is designed of rapid isolation of high-quality & high-quantity plasmid. The principle of plasmid purication of this kit is based on the alkaline-lysis method and the binding properties of DNA on the silica-gel membrane. The high-quality plasmids isolated from this kit is adequate for routine molecular biology application such as automated sequencing, cloning , PCR, in vitro transcription, transfection experiment etc. is also specially designed for high yield isolation of low-copy plasmid and much large size of circular DNA such as Cosmid or BAC clones. Spin column type Binding capacity: 20ug DNA/column Recovery yield: above 80%

Quality
Comparative quality test
Supplier A Innova Plex Supplier B High-copy number plasmid Low-copy number plasmid Large size of plamid

Automatic sequencing result

160

Band denslty (relative value)

140 120 100 80 60 40 20 0

Innova Plex Supplier A Supplier B High-copy Low-copy number number plasmid plasmid Large size of plamid

Products
Catalog No.
IPM01-C100 IPM01-C200

Product Name
Plasmid Mini-prep Kit Plasmid Mini-prep Kit

Units
100preps 200preps

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

PCR Purification Kit


Descriptions

for DNA clean up from PCR and other enzymatic reactions

Innova Plex PCR purication kit is designed for rapid and high-yield cleanup DNA from PCR and other enzymatic reaction mixture. The high purity DNA isolated using this kit is adequate for any molecular biology applications. Especially, the introduced unique system (DR condition) for the ecient removal of primer dimers, by-products of PCR, insures excellent results of automated sequencing and cloning. Spin column type DNA size: 70bp~20kb Recovery yield: above 80% Ecient removal of primer dimer

Benefits
One-step DNA clean-up from PCR or other enzymatic reactions Applicable to broad range of size(from 70 bp to 14kb) Two conditions are available for best results. Under the DR(dimer removal) condition, small fragment (less than 100bp) of DNA could be completely removed and you can obtain best results of automatic sequencing or cloning works. Under the HY(high yield) condition, all of the applicable size of DNA (from 70bp to 14kb)could be obtained with above 80% yield. High reproducibility and stability

Quality
Comparative quality test
Supplier A
O B A

Innova Plex
O B A Recovery Yield

1.2 1.0

90bp 300bp 1.5kb 4.0kb 14kb O. Original sample B. Condition B A. Condition A

0.8 0.6 0.4 0.2 0.0

Innova Plex Supplier A A B 90bp A B 300bp A B 1.5kb A B 4.0kb A B 14kb

Condition DNA size

Condition A: Innova Plex HY condition.2 volumes of isopropanol and 2 volumes of each binding buer were applied to 1 volume of original PCR mixture. Condition B: Innova Plex DR conditions.5 volumes of binding buers were applied to 1 volume of original PCR mixture.

Clear primer dimers


M O Supplier A Innova Plex M O Supplier A Innova Plex Direct sequencing of a PCR product puried using Innova Plex kit

M: size maker

O: original sample(before purication)

Products
Catalog No.
IPP02-C100 IPP02-C200

Product Name
PCR Purication Kit PCR Purication Kit

Units
100preps 200preps

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

Gel Extraction Kit


Descriptions

for high yield DNA isolation from agarose gel

Gel Extraction kit is designed for high-yield &high-quality isolation of any type of DNA(linear or circular size of 50 bp-20kb)from an agarose gel after gel electrophoresis. This kit is adequate for preparation of high quality DNA for automated sequencing , cloning, uorescent or radioactive labelling, in vitro transcription and other molecular biology applications. Spin column type DNA size:50bp~20kb recovery yield:above 80%(70bp~10kb) [Fragments of shorer than 700 bp or longer than 10kb show 50~70% yield]

Benefits
Easy and safe purication of DNA from agarose gels Preparation of highly puried DNA with highest yield Applicable to broad range of size( from 70 bp to 14kb) High reproducibility and stability

Quality
Comparative quality test
Supplier A Innova Plex Supplier B 90bp 200bp 300bp 500bp 700bp 1.2kb 1.5kb 4.0kb 14.0kb
1.2

Automatic sequencing result

Recovery Yield

1.0 0.8 0.6 0.4 0.2 0.0


90bp 200bp 300bp 500bp 700bp 1.2kb 1.5kb 4.0kb 14.0kb

Innova Plex Supplier A Size of eluted DNA

Products
Catalog No.
IGE03-C100 IGE03-C200

Product Name
Gel Extraction Kit Gel Extraction Kit

Units
100preps 200preps

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

Gel & PCR Purification Kit


Descriptions

for DNA clean up from PCR and other enzymatic reactions

Innova Plex Gel & PCR purication System Kit is designed to extract fragments of 50bp~20kb from an agarose gel after gel electrophoresis or to purify PCR products directly from a PCR reaction mixtures. The introduced unique system (DR condition) is highly ecient to remove the primer dimers, by-products of PCR . This kit can be used for preparation of high quality DNA for automated sequencing, cloning, uorescent or radioactive labeling, in vitro transcription and other molecular biology applications. Spin column type DNA Size : 50bp-20kb Recovery Yield : above 80%(70bp-10kb)

Benefits
Applicable to gel extraction and PCR purication by one kit High yield and high purity DNA purication High quality purication of DNA from 70bp to over 14kb

Compostion
UB : Universal buer WB : Washing buer(80% EtOH) Only empty bottle is provided because Ethanol is easy to volatilize or deteriorate. EB : Elution buer Spin column

Quality
PCR Purication
M C A-1 B-1 A-2 B-2 M

Gel Extraction
M C Q SG SU M C Q SG SM

glo-F-R1

M C Q

SG

SU

M C Q

SG

SM

glo-F-R3

glo-F-R5

PCR Purication M: 1kb DNA Ladder A-1: PCRB Dimer Removal B-1: Gel & PCR Dimer Removal A-2: PCRB HighYield B-2: Gel & PCR High Yield C: Control(original sample)

Gel Extraction M: 1kb DNA Ladder C: Control(original sample) Q: Q company sample SG : GEB prep SU: Gel & PCR prep

Products
Catalog No.
IGP04-C100 IGP04-C200

Product Name
Gel & PCR Extraction System Gel & PCR Extraction System

Units
100preps 200preps

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

Sequence Saver SolutionTM


to save Sequencing cost

Descriptions
Sequence Saver SolutionTM is a noble reagent for economic sequencing reactions of plasmids or PCR products. A good sequencing results can be obtained with extremely small amount of BigDyeTM (0.1250.25ul) or DYEnamicTM ET Terminator (0.25-0.5 ul) ready reaction mix by adding Sequence Saver SolutionTM into the reactions. With Sequence Saver SolutionTM the sequencing cost could be greatly reduced without compromising data quality. Sequence Saver SolutionTM is fully applicable In the BigDyeTM or DYEnamicTM ET Terminator sequencing reactions and suitable for analysis using automatic sequencers.

Benefits
Saving cost for sequencing of plasmids or PCR products. Reliable and stable results without compromising data quality Easy adaptation to your bulk-sequencing reaction system.

Quality
Plasmid(pGEM vector) sequencing
Template: Plasmid Template: PCR product

Big dye 0.125 ul Big dye 0.25 ul Big dye 1.0 ul

Big dye 0.125 ul Big dye 0.25 ul Big dye 1.0 ul

Big dye 0.125 ul Big dye 0.25 ul Big dye 1.0 ul Fig 1. Sequence Save Solution Eect of DNA Sequencing Sequence Saver SolutionTM Eect of DNA Sequencing Template: plasmid sequencing (150-200ng/ul) PCR product sequencing (10-20ng/ul) 1:3 Diluted BigDyeTM Mix (Big Dye: Dilution Buer = 1:3) Sequence Saver SolutionTM Amplication cycle: 30cycles Reaction volume : 10ul

Products
Catalog No.
ISS31-B800 ISS31-B32h ISS31-B16k

Product Name
SEQUENCE SAVER SolutionTM /800rxns Sequence Saver SolutionTM /3,200rxns Sequence Saver SolutionTM /16,000rxns

Units
1.6ml 6.4ml 32ml

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

2X Real-Time PCR Pre-mix


Descriptions
Real-Time PCR premix is Innova Plexs own hx-Taq DNA Polymerase. It invented as a 2X PCR premix type for convenient usage for user. Real-Time PCR premix can reduce precious study time. Just perform the PCR with DNA polymerase, dNTP, reaction buer mixture in advance and then put included Innogreen, primer and template in the kit.

Compositions
2X Real Time PCR premix 1ml*4 20X Innogreen 500ul*1

Quality
Sensitive Quanticaiton of Target Gene

HBV strong positive control DNA serial dilution (an undiluted solution(1), 1/5, 1/10, 1/100 - standard) NTC = Negative control (No template).

Specic and sensitive Quantication of Target Gene

(User Data) qPCR for cDNA form cold-treated Arabidopsis (Targeted to a cold -responsive gene) E** - Green dye

A1 and B1, control(untreated) A2 and B2, 3hours cold-treated A3 and B3, 24hours cold-treated

Products
Catalog No.
IRH 71-M40h

Product Name
2X Real-Time PCR Premix (25mM MgCl2), Innogreen 500ul added

Units
4ml

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

Reverse Transcriptase RNase HDescriptions


Innova Plex Reverse Transcriptase is a recombinant enzyme puried from an E.coli strain harboring a plasmid with a part of pol gene encoding Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase. The enzyme exhibits an RNA-directed DNA polymerase activity but lacks ribonuclease H activity. Innova Plex Reverse Transcriptase also contains a special thermo-stable chaperon which makes the reverse transcriptase more stable and active at high temperature.

Quality
Innova Plex Supplier A Supplier B
1. Total RNA 50ng 2. Total RNA 25ng 3. Total RNA 12.5ng 4. Total RNA 6.5ng 5. Total RNA 3.2ng 6. Negative control (without RNA)
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6

Products
Catalog No.
IOR51 E20h

Product Name
Reverse transcriptase(RNase H-)

Units
10000 U

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

M-MLV Rtase(RNase-)
Descriptions
Innova Plex Reverse Transcriptase is a recombinant enzyme puried from an E.coli strain harboring a plasmid with a part of pol gene encoding Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase. The enzyme exhibits an RNA-directed DNA polymerase activity but lacks ribonuclease H activity. Innova Plex Reverse Transcriptase also contains a special thermo-stable chaperon which makes the reverse transcriptase more stable and active at high temperature.

Quality
Innova Plex Supplier A Supplier B
1. Total RNA 50ng 2. Total RNA 25ng 3. Total RNA 12.5ng 4. Total RNA 6.5ng 5. Total RNA 3.2ng 6. D.W
1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6

Products
Catalog No.
IMR 01 R10k

Product Name
M-MLV Rtase (RNase H-)

Units
10000 U

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

Genomic DNA Prep Kit


Descriptions
Innova Plex Genomic DNA Prep Kit simplies isolation of DNA from samples (tissue and cell) with fast spincolumn. No phenol or chloroform extraction is required. DNA binds specically to the Innova Plex spin-column while contaminants pass through. PCR inhibitors such as divalent cations and proteins are completely removed in two simple wash steps, leaving pure DNA to be eluted in either water or buer provided with the kit. Innova Plex DNA technology yields genomic DNA from animal tissue, plant tissue, bacteria, yeast, blood samples ready to use in PCR and blotting prodedures. The convenient spin-column procedure means that hands-on preparation time is only 25minutes. Samples can be processed using a microcenrifuge. Applicable to isolation of genomic DNA from various tissue and cell samples by one single kit.

Advantages
Fast & high yield No phenol, no chloroform High quality, less fragmentation Low cost & highest services Isolation of gDNA from various samples by one kit

Quality
Animal Tissue - 50mg Muscle Tissue A Q
M

B I Q I

Mouse Organic Tissue Muscle Tall


M Q I M 1

Q
2 3 1

I
2 3

Q : Company Q Genomic DNA Prep Kit I : Innova Plex A : Pig muscle B : Hanow muscle

1. Liver 2. Kidney 3. Duodenum

Q.C test
A B C M

A: Wild B: Hetro C: Mutant

1
INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

Genomic DNA Prep Kit


Blood Blood Sample : 200 ul Q I
M

Company Q Kit Q
A B C

Innova Plex Kit Q


D M A B C

I
D

Q : Company Q Genomic DNA Prep Kit I : Innova Plex

A : 0.5kb B : 1kb C : 2kb D : 4kb Template : Human gDNA

Bacteria
M Q I

Q.C Test aroE (0.8kb)


M Q I M

aroK (1.7kb)
Q I

Wild type E.coli : 500ul(1X108) Template : E.coli Genomic DNA Primer : aroE F/R, aroK F/R

Yeast
M A B C D E F M

Genomic DNA separation result from Yeast(2X107 cell/ml) using Innova Genomic DNA Prep kit(2X107) A, D : 0.5ml cel + lyticase(2.5u) B, E : 1ml cel + lyticase(2.5u) C, F : 2ml cel + lyticase(2.5u)

Products
Catalog No.
IGD41-C050 IGD41-C100

Product Name
Genomic DNA Prep Kit(blood, bacteria, plants, animal tissue ) - column type Genomic DNA Prep Kit(blood, bacteria, plants, animal tissue ) - column type

Units
50preps 100preps

2
INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

AceStarTM Taq DNA Polymerase


Descriptions
Innova Plex AceStarTM Taq Polymerase was extracted and puried from a cloned and modied recombinant type E.Coli with DNA Polymerase gene of Thermus aquaticus. It employs Anti-Taq Antibody to exhibit advantages of both hot start PCR enzyme and general Taq Enzyme.

Benefits
Superior LOD, eecive in amplicaiton of low copy DNA High activity, High sensitivity Even more eective than Taq DNA Polymerase in multiplex PCR condition setup.
*LOD(Limit of Detection) : Method to measure amount of the minimum sample concentration needed in PCR.

Quality
Sensitivitiy Test
M A B C D E F G M M A B C D E F G M

Taq 1.25 unit

AceStarTM Taq 1.25 unit

M : 1kb* DNA Ladder A : Template 10ng B : Template 2ng C : Template 0.4ng D : Template 0.08ng E : Template 0.016ng F : Template 0.008ng G : Negative (without Template)

Activity Test
M A B C D E F M

Company G
Template : Bovine Genomic DNA M : 1kb* DNA Ladder A : Enzyme 2.5unit B : Enzyme 1.25unit C : Enzyme 0.625unit D : Enzyme 0.3125unit E : Enzyme 0.15625unit F : Negative (without Enzyme)

Hs-Taq

Products
Catalog No.
IDT16-R500 IDT16-R25h IDT16-R50h

Product Name
AceStarTM Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 0.4ml) AceStarTM Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 2.0ml) AceStarTM Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 4.0ml)

Units
500U 2,500U 5,000U

INNOVAPLEX

Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 kimlee@innovaplex.com http://www.innovaplex.com

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