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TYPES OF CULTURE MEDIA Media are of different types on consistency and chemica composition! A! On Consistency" #! So id Media!

Ad$anta%es of so id media" &a' (acteria may )e identified )y st*dyin% the co ony character+ &)' Mi,ed )acteria can )e separated! So id media is *sed for the iso ation of )acteria as p*re c* t*re! -A%ar- is most common y *sed to prepare so id media! A%ar is po ysaccharide e,tract o)tained from sea.eed! A%ar is an idea so idifyin% a%ent as it is " &a' (acterio o%ica y inert+ i!e! no inf *ence on )acteria %ro.th+ &)' It remains so id at /01C+ and &c' It is transparent! 2! Li3*id Media! It is *sed for prof*se %ro.th+ e!%! ) ood c* t*re in i3*id media! Mi,ed or%anisms cannot )e separated! (! On Chemica Composition " #! Ro*tine La)oratory Media 2! Synthetic Media! These are chemica y defined media prepared from p*re chemica s*)stances! It is *sed in research .or4! ROUTI5E LA(ORATORY MEDIA These are c assified into si, types" &#' (asa media+ &2' Enriched media+ &/' Se ecti$e media+ &6' Indicator media+ &7' Transport media+ and &8' Stora%e media! #! (ASAL MEDIA! (asa media are those that may )e *sed for %ro.th &c* t*re' of )acteria that do not need enrichment of the media! E,amp es" 5*trient )roth+ n*trient a%ar and peptone .ater! Staphy ococc*s and Entero)acteriaceae %ro. in these media! 2! E5RIC9ED MEDIA! The media are enriched *s*a y )y addin% ) ood+ ser*m or e%%! E,amp es" Enriched media are ) ood a%ar and Lo.enstein:;ensen media! Streptococci %ro. in ) ood a%ar media! /! SELECTI<E MEDIA! These media fa$o*r the %ro.th of a partic* ar )acteri*m )y inhi)itin% the %ro.th of *ndesired )acteria and a o.in% %ro.th of desira) e )acteria! E,amp es" MacCon4ey a%ar+ Lo.enstein:;ensen media+ te *rite media &Te *rite inhi)its the %ro.th of most of the throat or%anisms e,cept diphtheria )aci i'! Anti)iotic may )e added to a medi*m for inhi)ition! 6! I5DICATOR &DIFFERE5TIAL' MEDIA! An indicator is inc *ded in the medi*m! A partic* ar or%anism ca*ses chan%e in the indicator+ e!%! ) ood+ ne*tra red+ te *rite! E,amp es" ( ood a%ar and MacCon4ey a%ar are indicator media! 7! TRA5SPORT MEDIA! These media are *sed .hen specie:men cannot )e c* t*red soon after co ection! E,amp es" Cary:( air medi*m+ Amies medi*m+ St*art medi*m!

8! STORA=E MEDIA! Media *sed for storin% the )acteria for a on% period of time! E,amp es" E%% sa ine medi*m+ cha 4 coo4ed meat )roth! Pict*re COMMO5 MEDIA I5 ROUTI5E USE 5*trient (roth! 7>> % meat+ e!%! o, heart is minced and mi,ed .ith # itre .ater! #> % peptone and 7 % sodi*m ch oride are added+ p9 is ad?*sted to 0!/! Uses" &#' As a )asa media for the preparation of other media+ &2' To st*dy so *) e prod*cts of )acteria! 5*trient A%ar! It is so id at /01C! 2!7@ a%ar is added in n*trient )roth! It is heated at #>>1C to me t the a%ar and then coo ed! Peptone Aater! Peptone #@ and sodi*m ch oride >!7@! It is *sed as )ase for s*%ar media and to test indo e formation! ( ood A%ar! Most common y *sed medi*m! 7:#>@ defi)rinated sheep or horse ) ood is added to me ted a%ar at 67:7>1C! ( ood acts as an enrichment materia and a so as an indicator! Certain )acteria .hen %ro.n in ) ood a%ar prod*ce haemo ysis aro*nd their co onies! Certain )acteria prod*ce no haemo ysis! Types of chan%es " &a' )eta &p' haemo ysis! The co ony is s*rro*nded )y a c ear Bone of comp ete haemo ysis+ e!%! Streptococc*s pyo%enes is a )eta haemo ytic streptococci+ &)' A pha &a' haemo ysis! The co ony is s*rro*nded )y a Bone of %reenish disco o*ration d*e to formation of )i i$erdin+ e!%! <iridans streptococci+ &c' =amma &y' haemo ysis+ or+ 5o haemo ysis! There is no chan%e in the medi*m s*rro*ndin% the co ony+ Choco ate A%ar or 9eated ( ood a%ar! Prepared )y heatin% ) ood a%ar! It is *sed for c* t*re of pne*mococc*s+ %onococc*s+ menin%ococc*s and 9aemophi *s! 9eatin% the ) ood inacti$ates inhi)itor of %ro.ths! MacCon4ey A%ar! Most common y *sed for entero)acteriaceae! It contains a%ar+ peptone+ sodi*m ch oride+ )i e sa t+ actose and ne*tra red! It is a se ecti$e and indicator medi*m " &#' Se ecti$e as )i e sa t does not inhi)it the %ro.th of entero)actericeae )*t inhi)its %ro.th of many other )acteria! &2' Indicator medi*m as the co onies of )acteria that ferment actose ta4e a pin4 co o*r d*e to prod*ction of acid! Acid t*rns the indicator ne*tra red to pin4! These )acteria are ca ed - actose fermenter-+ e!%! Escherichia co ! Co o*r ess co ony indicates that actose is not fermented+ i!e! the )acteri*m is non: actose fermenter+ e!%! Sa mone a! Shi%e a+ <i)rio! M*e er 9inton A%ar! Disc diff*sion sensiti$ity tests for antimicro)ia dr*%s sho* d )e carried o*t on this media as per A9O recommendation to promote

reprod*ci)i ity and compara)i ity of res* ts! 9iss-s Ser*m Aater Medi*m! This medi*m is *sed to st*dy the fermentation reactions of )acteria .hich can not %ro. in peptone .ater s*%ar media+ e!%! pne*mococc*s+ 5eisseria+ Coryne)acteri*m! Lo.enstein:;ensen Medi*m! It is *sed to c* t*re t*)erc e )aci i! It contains e%%+ ma achite %reen and % ycero ! &#' E%% is an enrichment materia .hich stim* ates the %ro.th of t*)erc e )aci i+ &2' Ma achite %reen inhi)its %ro.th of or%anisms other than myco)acteria+ &/' = ycero promotes the %ro.th of Myco)acteri*m t*)erc* osis )*t not Myco)acteri*m )o$is! Pict*re D*)os Medi*m! This i3*id medi*m is *sed for t*)erc e )aci i! In this medi*m dr*% sensiti$ity of t*)erc e )aci i can )e carried o*t! It contains -t.een C>-+ )o$ine ser*m a )*min+ casein hydro ysate+ aspara%in and sa ts! T.een C> ca*ses dispersed %ro.th and )o$ine a )*min ca*ses rapid %ro.th! Loeff er Ser*m! Ser*m is *sed for enrichment! Diphtheria )aci i %ro. in this medi*m in 8 ho*rs .hen the secondary )acteria do not %ro.! It is *sed for rapid dia%nosis of diphtheria and to demonstrate $o *tin %ran* es! It contains sheep+ o, or horse ser*m! Te *rite ( ood A%ar! It is *sed as a se ecti$e medi*m for iso ation of Cotyne)acteri*m diphtheriae! Te *rite inhi)its the %ro.th of most secondary )acteria .itho*t an inhi)itory effect on diphtheria )aci i! It is a so an indicator medi*m as the diphtheria )aci i prod*ce ) ac4 co onies! Te *rite meta)o iBed to te )rism+ .hich has ) ac4 co o*r! EM( &Eosin:methy ene ) *e' A%ar! A se ecti$e and differentia medi*m for enteric =ram:ne%ati$e rods! Lactose:fermentin% co onies are co o*red and non actose: fermentin% co onies are nonpi%mented! Se ects a%ainst %ram positi$e )acteria! DLD &Dy ose Lysine Deo,ychoiate'! It is *sed to iso ate Sa mone a and Shi%e a species from stoo specimens! This is a se ecti$e media! SS &Sa mone a:Shi%e a' A%ar! It is a se ecti$e medi*m *sed to iso ate Sa mone a and Shi%e a species! SS A%ar .ith additiona )i e sa t is *sed if Yersinia enteroco itica is s*spected! DCA &Deso,ycho ate Citrate A%ar'! It is *sed for iso ation of Sa mone a and Shi%e a! The other enteric )acteria are most y inhi)ited &a se ecti$e medi*m'! It is a so a differentia &indicator' medi*m d*e to presence of actose and ne*tra red! Tetrathionate (roth! This medi*m is *sed for iso atin% Sa mone a from stoo ! It acts as a se ecti$e medi*m! It inhi)its norma intestina )acteria and permits

m* tip ication of Sa mone a! Se enite F (roth! Uses and f*nctions are same as that of tetrathionate )roth! Pict*re Thios* phate:Citrate:(i e:S*crose &TC(S' A%ar! TC(S a%ar is a se ecti$e medi*m *sed to iso ate <i)rio cho erae and other <i)rio species from stoo ! Charcoa :yeast a%ar! Used for Le%ione a pne*mophi a! Increased concentration of iron and cysteine a o.s %ro.th! Te *rite:=e atin A%ar Medi*m &T=AM'! It may )e *sed as transport+ se ecti$e and indicator medi*m! A 4a ine peptone .ater! See *nder <i)rio! &Chapter 7#'! Campy o)acter Medi*m! This se ecti$e medi*m is *sed to iso ate Campy o)acter ?e?*ni and Campy o)acter co i from stoo ! Cary:( air Medi*m! It is *sed as a transport medi*m for faeces that may contain Sa mone a+ Shi%e a+ <i)rio or Campy o)acter species! Amies medi*m is *sed for %onococci and other patho%ens! Peptone Aater S*%ar Media! These indicator media are *sed to st*dy -S*%ar fermentation-! # @ so *tion of a s*%ar & actose+ % *cose+ mannito etc' is added to peptone .ater containin% Andrade-s indicator in a test t*)e! A sma in$erted D*rham t*)e is p aced in the medi*m! The media are co o*r ess! After c* t*re+ chan%e of a medi*m to red co o*r indicates acid prod*ction! =as+ if prod*ced co ects in D*rham t*)e! Moti ity Indo e Urea &MIU' Medi*m! This is *sed to differentiate entero)acteria species )y their moti ity+ *rease+ and indo e reactions! TSI &Trip e s*%ar iron' A%ar: See chapter 7/! EIA &E i% er Iron A%ar'! This is a differentia s ope medi*m *sed in the identification of enteric )acteria! The reactions are )ased on the fermentation of actose and % *cose and the prod*ction of hydro%en s* phide &chapter 7/'! Christensen-s Urea Medi*m! This is *sed to identify *rea sp ittin% or%anisms+ e!%! Prote*s! A p*rp e pin4 co o*r indicates *rea sp ittin% &See chapter 7/'! (ordet:=en%o* Medi*m! This medi*m is *sed for c* t*re of (ordete a pert*ssis! Increased concentration of ) ood a o.s %ro.th! It contains a%ar+ potato+ sodi*m ch oride+ % ycero + peptone and 7>@ horse ) ood! Penici in may )e added to it! L( (roth and L( A%ar Most referenced )acteria media for %ro.th and

maintenance of recom)inant E! co i strains Terrific (roth 5*tritiona y rich )acteria media for hi%her density %ro.th and maintenance of recom)inant E! co i strains MF Minima Common y *sed )acteria media for c* ti$ation and maintenance of E! co i strains! Ma%icMediaG Medi*m C*stomiBed )acteria media for %ro.th of T0: re%* ated E! co i strains for a*to ind*ction of protein e,pression ImMediaG Medi*m Pre:mi,ed+ pre:steri iBed )acteria media containin% se ection anti)iotics for %ro.th of E! co i strains Other Media Micro)ia media for o*t%ro.th of transformed E!co i ce s &S!O!C! Medi*m'+ so id micro)ia media &Se ect A%arH'+ and %ro.th of hosts for rep ication of M#/ $ectors &2:YT (roth'

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