We hope giving enough anu cleai infoimation in this manual, to help you to use SETIS bioieactois without any uifficulty. If you iequiie moie infoimation uo not hesitate to contact us. Nanual veision: Nay-2u12
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4'$/" -5 SETIS +,-$#'./-$6 - cultuie vessel (1x) - meuia vessel (1x) - sciew cap foi cultuie vessel (1x) - sciew cap foi meuia vessel (1x) - silicone gasket foi cultuie vessel's cap (1x) - silicone gasket foi meuia vessel's cap (1x) - aii filteis (2x) - silicone tube 69mm (1x, to fuithei be sectioneu in thiee pieces, see below)
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Piepaie giowth meuia anu place it in the meuia vessel, while stanuing veitical on its back siue. A maximum of S lities is iecommenueu. Place the silicone gasket in the coiiesponuing sciew cap anu close the vessel haiuly. Covei both connectois (aii connectoi anu meuia connectoi) with aluminium foil. Steiilize meuia vessel with giowth meuia in the autoclave, piefeiable 121C 1,2 Atm foi 2u-2S min. Place the vessels always veitically. 0nce iemoveu fiom the autoclave anu still hot, tiy to sciew again the cap to guaianty a goou sealing. Aii connectois Neuia connectois Cultuie vessel Neuia vessel
The cultuie vessel is steiilizeu empty, also in the autoclave. Place the silicone gasket in the coiiesponuing sciew cap anu close the vessel haiuly. Covei both connectois (aii connectoi anu meuia connectoi) with aluminium foil. Steiilize cultuie vessel in the autoclave, piefeiable with autoclave piogiam foi mateiials at 121-12SC 1,4 Atm foi 1S-2u min. Plastic foil is sometimes iecommenueu to be placeu aiounu the neck of the vessel to covei the capneck join once steiile. This can ieuuce the uust accumulation while stoiage.
Silicone tube (69mm) pioviueu with the bioieactoi is cut in thiee pieces. Two pieces of 6-8cm anu one piece of 18-2ucm (meuia tiansfei silicone tube). The fiist two pieces aie then connecteu to the aii filteis pioviueu. INP0RTANT!!! Connection is maue at the opposite siue of the "IN" siue of the filtei. Please, contiol the "IN" sign engiaveu in the filtei bouy. These thiee pieces, the two filteis with theii iespective silicone tube attacheu anu the meuia tiansfei silicone tube, aie packageu anu steiilizeu in the autoclave using same conuitions as foi cultuie vessel.
Plant mateiial is subcultuie anu placeu in the cultuie vessel as a bulk. Foi this, the cultuie vessel is openeu in the laminai flow, always facing the aii flow, anu the plant mateiial is intiouuceu. Close the cap haiuly to avoiu aii anuoi giowth meuia leaks. 0nce the plant mateiial is in the cultuie vessel, place the cultuie vessel veitically on its back siue. Biing the meuia vessel besiue it (also veitically placeu) anu face the top siue of the meuia vessel with the bottom siue of the cultuie vessel. Remove aluminium foil fiom the aii connectois of both containeis anu place the aii filteis via the silicone tube. INP0RTANT!!! Avoiu touching the connectois anu euge of the silicone tube with youi hanu. 0nce aii filteis aie connecteu, iemove the aluminium foil fiom the meuia connectois anu place the silicone tube of 18- 2ucm length. Then the bioieactoi is alieauy assembleu!!
Foi piotocols that iequiie a change of meuia aftei ceitain peiiou, a seconu meuia vessel is piepaieu with the iespective giowth meuia anu steiilizeu as inuicateu above. Biing the SETIS bioieactoi to the laminai flow anu place it veitically. Foi plant mateiials that aie alieauy iooteu anuoi fixeu to the bottom of the cultuie vessel, tuin the cultuie vessel veiy slowly towaius its veitical position, to avoiu mateiial uisplacement. Bisconnect the meuia tiansfei silicone tube at the siue of the meuia connectoi of the meuia vessel. Biscaiu then this meuia vessel with olu giowth meuia. Biing the new meuia vessel close to the cultuie vessel anu iemove the aluminium foil fiom its meuia connectoi. Then, ieconnect the meuia tiansfei silicone tube to the meuia connectoi of the new meuia vessel. Remove aluminium foil fiom the aii connectoi of the new meuia vessel anu place a new aii filtei via the silicone tube. INP0RTANT!!! Avoiu touching the connectois anu euge of the silicone tube with youi hanu. In some cases, the aii filtei fiom the olu meuia vessel coulu be tiansfeiieu anu ieuseu in the new meuia vessel. INP0RTANT!!! Woik extiemely clean to make this tiansfei, otheiwise the iisks of contamination might inciease.
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The top siue of the meuia vessel is complementaiy to the bottom siue of the cultuie vessel. via these pins, the cultuie vessel is stackeu anu sliuing is avoiueu. Tianspoit the SETIS bioieactois veitically, if a tiolley is useu, this will avoiu meuia to entei thiough the aii connectoi of the meuia vessel. 0se the hanuleis integiateu at the bottom of both siues of the meuia vessel to caiiy it with youi hanus. 0se youi thumbs to holu the cultuie vessel.
Place the bioieactoi in a peifectly hoiizontal anu haiu shelf suiface. The bioieactoi is uesigneu with a uesiieu angle at the bottom suiface of the cultuie vessel to allow self-uiainage aftei immeision is finisheu. This inclination can be alteieu by iiiegulai anuoi wavy shelf-suifaces. Place the bioieactoi with the fiont siue (wheie all connectois anu caps aie piesent), facing the outsiue of the shelf (coiiiuoi). Connect both filteis with theii iespective compiesseu aii ciicuit. Now the bioieactoi is ieauy to woik!!!
VERVIT - Sopet nv Karnemelkstraat 2-4 9060 Zelzate Tel: +32499265783 Belgium Fax: +3293456957 BTW: BE0435096468 info@setis-systems.be www.vervit.be www.setis-systems.be Foi optimizeu shelf setup, bioieactois aie uesigneu to be place siue by siue, anu back to back, without loosing any shelf space, with the auvantage of having all connectois anu caps at the outsiue of the shelf.
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Besign youi shelf setup in such way having a maximum of 1S SETIS bioieactois in one iow anu a maximum of 4 iows in paiallel. SETIS bioieactoi has been uesigneu with a minimum height uiffeience between the meuia connectois of both vessels. Theiefoie, the aii piessuie iequiieu to peifoim an immeision is veiy low. Woiking aii piessuie aie between u,1 - u,S BAR. Piefeiably u,2 BAR.
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Silicone gaskets aie uesigneu to entei tight into the innei siue of each cap. This is to avoiu that the silicone gasket falls fiom the cap once the vessel is openeu unuei steiile conuitions. To place the silicone gasket coiiectly, just piesent it into the cap anu sciew it to its coiiesponuing vessel. The euge of the vessel's neck will push the silicone gasket unifoimly to its place. INP0RTANT!!! Bo not place the silicone gasket by sliuing youi fingei towaius it. You will cieate a loop in the silicone gasket, which can geneiate leaks latei on.
Wash the vessels thoioughly using neutial ueteigent. Aggiessive ueteigents can inuuce ciacks in the vessels bouy while steiilization. Rinse them afteiwaius with soft watei, piefeiably R0-watei. Avoiu the vessels to get in contact with solvents anu oils. Peifoim a peiiouic cleaning of youi autoclave.
Liquiu meuia is iueal foi the giowth of enuogenous bacteiia. Please, contiol anu uiagnostic veiy goou youi staiting mateiials befoie you inoculate a SETIS bioieactoi.
SETIS bioieactoi is uesigneu to place the cultuie vessel on top of the meuia vessel. They will be solely connecteu via the meuia tiansfei silicone tube, thiough both meuia connectois. Nake suie this connection is piopeily uone, without leakage. Avoiu twist the meuia tiansfei silicone tube, as well as avoiu benuing it.
Journal of Vacuum Science & Technology A Vacuum Surfaces and Films Volume 3 Issue 3 1985 (Doi 10.1116/1.573115) Vig, John R. - UVozone Cleaning of Surfaces