Table 4.3 Showing positive growth over Pseudomonas isolation media.

Positive growth of the samples

Station Road Sample no.1 (New Cake House), Sample no.4 (Krishna Bakery)

Rama Mandi

Sample no.1 (Flora Bakery), Sample no.2 (Gurunanak Bakery) and Sample no. 4 (Ashwani Confectioner)

Jyoti Market

Sample no. 1(Friends Bakery), Sample no.3 (global Bakery Shop) and Sample no.4 (Gulshan Bakery)

Model town

Sample no.1 (Prakash Bakery)

Nakodar Chowk

Sample no.1 (Fancy Bakery) and Sample no.3 (DashmeshBakery)

Sample no.2 Station road

Sample no.4 Station road

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Sample no.1 Rama mandi

Sample no.4 Rama mandi

Sample no.2 Rama mandi

Sample no.1 Jyoti Market

Sample no.3 jyoti Market

Sample no.4 Jyoti Market

Sample no.1 Model Town

Sample no.1Nakaudar Chawk

Sample no.-3 Nakaudar Chawk

Fig4.1 Showing bacterial growth of Samples over the pseudomonas selective media collected from different areas.

Preparation of Pure Culture for Pseudomonas aeruginosa

For preparing pure culture single colony has been isolated and then suspended into the nutrient broth.

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Grams staining
Pseudomonas aeruginosa is a gram negative bacteria

Sample no.-2 Station Road

Sample no.-4 Station Road

Sample no.-1 Rama Mandi

Sample no.-2 Rama Mandi

Sample no.-4 Rama Mandi

Sample no.-1Jyoti Market

Sample no.-3 Jyoti Market

Sample no.4 Jyoti Market

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Sample no.-1Model Town

Sample no.-1Nakaudar Chawk

Sample no.-3 Nakaudar Chawk

Fig 4.2All figures showing grams negative results

Biochemical Tests
Biochemical tests has been done for cross checking the presence of this bacteria

Gelatin Hydrolysis
It gives positive result on gelatin hydrolysis which had been visualised by liquefaction of gelatine agar.

Fig 4.3Showing positive results of Gelatin Hydrolysis

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Citrate Utilization Test

Colour changing from green to blue indicates that Pseudomonas aeruginosa were present in the sample.

Fig 4.4Showing positive results of Citrate Utilization Test

Confirmatory test for E.coli

Growth on selective media

Out of different isolated bacteria some were found to be grown on the EMB agar selective media

Table no. 4.4 showing positive growth over the EMB media

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Sample no.-3 Station Road

Sample no.2 Station Road

Sample no.-2 Rama Mandi

Sample no.-4 Rama Mandi

Sample no.3 Jyoti Market

Sample no.1 Jyoti Market

Sample no.2 Jyoti Market

Sample no.4 Jyoti Market

Sample no.1 Model Town

Sample no.2 Model Town

Sample no.4 Model Town

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Sample no.3 Model Town

Sample no.4 Nakaudar Chawk

Sample no.3 Nakaudar Chawk

Fig 4.5 Showing bacterial growth of Samples over the EMB media collected from different areas.

Preparation of Pure Culture for E. coli

For preparing pure culture single colony has been isolated and then suspended into the nutrient broth.

Grams Staining
E. coli is a gram negative bacteria.

Fig. 4.6 showing gram negative bacteria by doing Grams staining.

After Grams staining 14 samples were found as negative bacteria

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Biochemical test
Biochemical tests has been done for cross checking the presence of this bacteria

Indole Test

Fig 4.7 Showing positive results of IndoleTest

MethylRed-VogesProskauer TEST

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Fig 4.8 Showing positive results of Methyl RedTest

Fig4.9 showing negative results of Voges Proskauer Test for E. coli

Citrate Utilization Test

Fig 4.10 showing negative results of Citrate Utilization Test for E.coli

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Comparison with another bakery product (Biscuits)

For observing which part of the pastry is more contaminated compared with another bakery product biscuits. For this ,Parley G biscuits were chosen and the same protocol were followed as done in the case of Pastry sample .Further, cream and bakery part of the pastry were separately observed for bacterial growth.

Results

Fig 4.11 showing minimum growth over the NA agar plates from biscuits samples

Fig 4.12 showing no growth over the EMB agar plates from biscuits samples

Fig 4.13 showing no growth over the Pseudomonas isolation agar plates from biscuits samples

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For Pseudomonas aeruginosa Name of Area Positive growth over Positive growth over the media from cream the media from bakery part part Sample no.4(Krishna ------Bakery) Sample Bakery) Sample Bakery) no.2(Gurunanak no.

Station Road Rama Mandi Jyoti Market Model town Nakodar Chowk

------

1(Friends Sample no.3(global Bakery Shop)

--------Sample no.1(Fancy Bakery)

-------Sample no.3(DashmeshBakery)

Table no. 4.5 showing comparative results of growth over the Pseudomonas isolation media from creamy and bakery part of pastry

Fig4.14 showing positive growth over the Pseudomonas isolation agar plates, samples taken from the creamy part

Fig4.15 showing positive growth over the Pseudomonas isolation agar plates, samples taken from the Bakery part.

For E.coli

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Name of Area

Station Road Rama Mandi Jyoti Market Model town Nakodar Chowk

Positive growth over Positive growth over the media from the the media from the creamy part bakery part Sample no.1(New Cake ------------House)

--------------------------Sample no.1(Fancy Bakery)

----------Sample no.3(global Bakery Shop)

-----------Sample no.3(DashmeshBakery)

Table 4.6 Showing comparative results of growth over the EMB media from creamy and bakery part of pastry

Fig4.16 showing positive growth over the EMB agar plates, samples taken from the creamy part.

Fig4.17 showing positive growth over the EMB agar plates, samples taken from the bakery part.

Result
On the basis of this experiment, it can be said that the creamy part of the pastry is more susceptible for bacterial.

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