4.

Tripozoit Chilomastix mesnili

Ciri-ciri:
- Bentuk jambu monyet
- Inti 1 dengan karyosom
- Punya 3 flagel anterior dan 1 flagel halus dalam sitostoma
- Meruncing di bagian posterior

Chilomastix mesnili
Introduction
Chilomastix mesnili is cosmopolitan in distribution although found more frequently in
warm climates. It is thought to be non-pathogenic although the trophozoite has been
associated with diarrhoeic stool. This is the largest flagellate found in man with an
incidence of 1-10% being in the large intestine.
(Diag 6 illustrates the life cycle of C. mesnili)


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Morphology of the Trophozoite
The trophozoites of C. mesnili are pear shaped and measure 6-20 m in length.
They have 1 large nucleus with a small karyosome and 3 flagella that extend from
the nucleus at the anterior end of the parasite. A distinct oral groove or cytosome
can be seen near the nucleus with its sides being supported by two filaments. They
are known to move in a directional manner. (Diag 7 & Fig 7)








Morphology of cysts
The cysts are 6-9 m, they have a large single nucleus with a large
karyosome. They also have a prominent side knob giving it a characteristic lemon
shape. (Diag 8 & Fig 8) The cytosome is evident with a curved shepherds crook fibril.
It also has a characteristically coiled filament which when stained is darker in colour.







Fig 8. Chilomastix mesnili cysts are excreted with faeces and constitute the transmission form of the
micro-organism. The uninucleated lemon shaped cysts are seen with a little proturberance at one end
and a prominent cytostome. (Iodine stained). (6 m) (www.dpd.cdc.gov)
Laboratory Diagnosis
The characteristic lemon shaped cysts can be seen in a formol-ether concentrate.
(Fig 8) Motile organisms can be seen in a wet preparation of a fresh stool however
the characteristic morphology is evident in a permanently stained preparation. (Keys
1 & 2) Table 1 (page 17) details useful morphological features that are similar
between species of flagellate and are used in laboratory diagnosis