Professional Documents
Culture Documents
1960 - The Rockefeller and Ford Foundation jointly established the first international agricultural
research center in cooperation with the Philippine government and the International Rice
Research Institute (IRRI).
(Link to IRRI )
1996 – IRRI released new rice that has been named "Super Rice"
because it is predicted to increase rice yields by 25-50%. It is
supposed to produce 15 metric tonnes per hectare.
(Link to Super Rice )
2002 – Researchers were able to sequence the DNA of rice, the main food source for two thirds
of the world’s population. It is the first crop to have its genome decoded.
(Link to Rice Genome )
2004 - United Nations dedicates 2004 to the world’s most important staple food once again,
celebrating it as the International Year of Rice, the starchy grain has undergone a complete
metamorphosis. Syngenta now owns patents for Golden Rice as well as BT Corn.
(Link to Oryza Syngenta ) (Link to Syngenta BT Corn )
Miracle Rice Group (L. Totten, F. Rodriquez, W. Montero, M. Daza-Enriquez)
Biotechnology Training March 5, 2005
Homework: Timeline
1957 – In 1957 Francis Crick and George Gamov worked out the
“Central Dogma”, explaining how DNA functions to make protein.
Their “sequence hypothesis” posited that the DNA sequence
specifies the amino acid sequence in a protein. They also suggested
that genetic information flows only in one direction, from DNA to messenger RNA to protein,
the central concept of the central dogma.
(Link to Central Dogma )
1965 – Scientist noticed that genes conveying antibiotic resistance in bacteria are often carried
on small, supernumerary chromosomes called plasmids. This observation led to the classification
of the plasmids.
(Link to Mechanisms of Evolution ) (Link to Antibiotic Paradox )
1966 – In 1966 the genetic code was finally “cracked”. Marshall Nirenberg, Heinrich Mathaei
and Severo Ochoa demonstrated that a sequence of three nucleotide bases, determines each of
the 20 amino acids.
(Link to 1966 Cracking Genetic Code )
1970 - Howard Temin and David Baltimore, first isolated "reverse transcriptase" a restriction
enzyme that cuts DNA molecules at specific sites. Their work described how viral RNA that
infects a host bacteria uses this enzyme to integrate its message into the host's DNA. This
discovery allowed scientists to create clones and observe their function. The major motivation to
understand reverse transcriptase comes from its role in HIV infections. The job of reverse
transcriptase in all retroviruses is to synthesize double-stranded DNA from the retrovirus' own
single-stranded RNA genome. This DNA double helix is then integrated into the host cell's
chromosomes as a provirus. Transcription then leads to copies of the viral RNA genome, from
which the virus's own proteins and enzymes are formed. New viral particles then bud from the
membrane of the cell. Thus, reverse transcriptase is essential for viral replication, becoming an
important target for drugs against AIDS.
(Link to Reverse Transcriptase )
1972 - Paul Berg isolated and employed a restriction enzyme to cut DNA.
Berg used ligase to paste two DNA strands together to form a hybrid circular
molecule. This was the first recombinant DNA molecule.
(Link to First Recombinant DNA )
(Link to Speaking the Language of Recombinant DNA )
(Link to Bio of Paul Berg )
1972 - The first successful DNA cloning experiments were performed in California.
(Link to PDF file of History of Genome Research )
1973 - Scientists for the first time successfully transferred deoxyribonucleic acid (DNA) from
one life form into another. Stanley Cohen and Annie Chang of Stanford University and Herbert
Boyer of UCSF "spliced" sections of viral DNA and bacterial DNA with the same restriction
enzyme, creating a plasmid with dual antibiotic resistance.
(Link to Recombinant DNA )
(Link to First Recombinant DNA Molecules )
(Link to Animation of Recombinant DNA Process )
1973 - The first human-gene mapping conference took place. The conference was inspired
primarily by the rapid development in mapping by somatic-cell hybridization.
(Link to Human-Rodent Somatic Cell Hybrids )
1974 – The National Academy of Sciences published a paper by Stanford geneticist Stanley
Cohen and UCSF biochemist Herbert Boyer in which they demonstrated the expression of a
foreign gene implanted in bacteria by recombinant DNA methods. Cohen and Boyer showed that
DNA can be cut with restriction enzymes and reproduced by inserting the recombinant DNA into
Escherichia coli.
(Link to The Basics of Recombinant DNA )
1977 – This is considered the Dawn of Biotechnology, Genentech produced the first human
protein (SOMATOSTATIN), manufactured in a microorganism (E. coli bacteria).
(Link to Genetech timeline )
(Link to UCSF Chronology of Biotechnology )
1982 – Genentech Inc., received approval from the FDA to market genetically engineered human
insulin. The first genetically engineered drug is a form of human insulin produced by bacteria.
(Link to Insulin )
(Link to the First Insulin )
1983 – Kary Mullis invented the polymerase chain reaction (PCR), a technique that amplifies
specific DNA sequences from very small amounts of genetic material. PCR has revolutionized
DNA technology by allowing scientists to produce an almost unlimited amount of highly
purified DNA molecules suitable for analysis or manipulation. The technique has widespread
applications, including the screening of genetic and infectious diseases, the reconstruction of
phylogenetic trees, and forensic analysis. An enzyme called POLYMERASE reads its code and
assembles a copy.
(Link to Biotechnology Advances )
(Link to DNA Timeline )