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Sidney G.

Azevedo
1,*
, Marcos B. Machado
1
, Raimundo Carlos Pereira Jr
1,2
, Elenn S. P. Aranha
1
, Ingrid Reis da Silva
2
,
Marne C. Vasconcellos
1
, Emerson S. Lima
1
, Rudi E. L. Procpio
2

1
UFAM - Universidade Federal do Amazonas (69077-000, Manaus/AM, Brazil)

2
CBA - Centro de Biotecnologia da Amaznia (69075-351, Manaus/AM, Brazil)
*sidneyazevedo1@gmail.com
Chemical characterization and biological evaluation of the essential oils from leaves
of Eugenia tapacumensis (O Berg) and Eugenia diplocampta (Diels).
CONCLUSIONS
Code of Samples
Cell lines (CI50 g/ mL)
MRC-5 MCF-7 HCT-116 SKMEL 19
E.T 33,21
(31,05 -35,52)
21,54
(16,25 - 28,57)
26,99
(22,22 - 32,80)
> 50
E D1-1 > 50 35,90
(28,13 - 45,81)
23,63
(16,30 34,24)
> 50
ED2-1 > 50 20,81
(12,92 33,50)
20,81
(12,92 33,50)
> 50
ED2-2 > 50 32,58
(23,99 - 44,25
32,63
(29,22 36,43)
> 50
Code of
Samples
Staphylococcus
aureus
Streptococcus
pneumoniae
Enterococcus
faecalis
Burkholderia
cepacia
Klebsiella
pneumoniae
Escherichia
coli
ET +++
+++

+++

+++

+++

+++

ED1-1 - - ++ - - -
ED2-1 +++ +++ ++ +++ - +++
ED2-2

+++

++

++

+++

-
+++
Table 2. Bioactivity assays diffusion method in agar discs
low (+): Diameter of inhibition zone between 0.7 and 1.2 cm; moderate (++): diameter of inhibition zone between 1.3 and 1.6 cm; High (+++):
diameter of inhibition zone greater than 1.7 cm. Negative (-): absence of inhibition zone;
Table 3. Cell viability by the Alamar blue assay. The values show CI50 (confidence interval).
Essential oils of ET and ED have common constituent (caryophyllene oxide,
copaene, and humulene epoxide II) and distinct (-bisabolene, b-elemene, -
humulene, and ledol) in its composition, as well as have a significant microbial and
cytotoxic activities.
Figure 02: Chromatographic profile of ET, ED1-1, ED2-1 and ED2-2 obtained by GC-MS.
REFERENCES
1-Ribeiro, J.R. et al. Flora de Reserva Ducke. INPA D-FID 1999.
2- Lima,T. P.et.al. Evaluation Of Chemical Composition Of Essential Oils Of Species Of Family Myrtaceae Native
Cerrado With The Potential Of Economic Use. UNICAMP, Campinas-SP
3-di Stasi, L.C.; Hiruma-Lima, C.A. Plantas Medicinais na Amaznia e na Mata Atlntica, 2nd ed.; Editora Unesp: So
Paulo, Brazil, 2002.

Figure 04: Biplot (scores and loadings of PC1vsPC2).
Figure 05: HCA of ET, ED1-1.ED2-1, and ED2-2
(Ward's method using Squared Euclidean Distance).
RESULTS AND DISCUSSION
Table 01. The major compounds identified in the
essential oils from Eugenia spp.
Major compounds ET
(%)
ED1-1
(%)
ED2-1
(%)
ED2-2
(%)
caryophyllene oxide 34.2 25.8 22.3 23.0
E-caryopillene 0.0 9.1 11.2 8.6
copaene 8.9 8.5 10.7 10.3
spathulenol 0.0 5.6 2.7 2.5
- bisabolene 8.7 0.0 0.0 0.0
epoxide humulene II 2.6 2.1 2.2 2.6
E verbenol 2.3 0.0 0.0 0.0
-elemene 0.0 3.2 0.0 0.0
cubebene 0.0 3.1 8.5 7.6
E calamenene 0.0 0.0 5.6 6.2
humulene 0.0 0.0 2.3 0.0
ledol 0.0 0.0 0.0 2.1
NEQUIMA
Ncleo de Estudos Qumicos em
Myrtaceae da Amaznia
CBA
CENTRO BIOTECNOLOGICO DA
AMAZNIA
Investigate the chemical composition of the essential oils from the leaves of Eugenia tapacumensis (O
Berg.) (ET) and E. diplocampta (Diels) (ED), and to evaluate the bacterial and cytotoxic activities of
these species.
INTRODUCTION
METHODOLOGY
Eugenia is one of the largest genera of the Myrtaceae family, its species are known to be rich in
volatile oils and for use in traditional medicine. In Brazil, Eugenia spp. occurs mainly in the
Atlantic Forest, Restinga Forest, and Amazon biome. In the Adolpho Ducke Forest Reserve
(RFDA) in Manaus/AM, where were identified 28 species of Eugenia
1
. Chemical studies with
species of Eugenia have shown a composition rich in flavonoids/glycosides, hydrolysable
tannins, coumarins, anthocyanins, monoterpenes/ sesquiterpense, and triterpenes /steroids
2
.
Biological studies involving essential oils of Eugenia spp. have been confirmed several
biological activities (antibacterial, antifungal, antitumor, and antioxidant)
3
.

OBJECTIVE
Figure 01: Adolpho Ducke Forest Preserve (RFDA).
Dried separately
Grinding and
hydrodistillation
with modified
Clevenger
Obtaining of
essential oils
Branches of Eugenia spp.
colllected in RFAD
Assay Biological:
1- Antimicrobial,
2- Cytotoxic.
cells
0.5 x10
4
cells/poll
24h, 37C 15% CO
2
Introduction
sample
10 L
Alamar blue
0,02%
Incubation
72h/37C/5% of CO
2
Spectrophotometer (excitation: 530 nm,
emission: 590 nm).
Antibiotic
gar Mller-Hington
Liquid culture mediums
Tube N 0.5
Mc Farland scale
25 L of the simple
Introduction of
microorganisms
Incubation period the 37 C
2
Leaves of Eugenia spp.
GC-MS
GC-FID
Essential oil
1
A detailed analyze of the loadings map (PC1vsPC2) have demonstrated E-vebenol and -
bisabolene as chemical characteristic for ET, and E-caryophyllene, spathulenol, and -
cubenene for ED. The HCA have showed a distinct separation in two groups (species)
and two sub-groups (individuals and collection period). ED1-1 and ED2-1/2 have -
elemene and E-calamenene as characteristic in the chemical composition, and have
been distinguished by the presence of -humulene and ledol, respectively.
Figure 03: Structures of the major compounds of
essential oils from Eugenia spp..