Kurs ETCS Mglichkeit zu Anerkennung

Cell Factories: Design,

engineering and analysis
10 keine
Recovery and purification of
biological products
5 Bioproduktenaufarbeitung 2
Advanced Enzyme Technology 5 Technische Biokatalyse
Experimental Molecular Biology of
Eukaryotes
5 Zellulre Biochemie
(Biochemisches Praktikum)


























Kursbeschreibungen
27405 Cell factories: Design, engineering and analysis

Danish title:
Cellefabrikker: Design, konstruktion og analyse
Language:

English
Point( ECTS )

10
Course type:
MSc
Taught under open university

Technological Specialization Course, Bioinformatics and Systems Biology
Technological Specialization Course, Biotechnology
Technological Specialization Course, Chemical and Biochemical Engineering

Schedule: E4 (tues/fri)

Course holdings

Location:
Campus Lyngby
Scope and form: Lectures, group exercises, seminars, and problem solving
Duration of Course: 13 weeks
Date of examination: E4A, F4A
Type of assessment: Oral examination
without preparing time - all exam questions are available approx. 2 weeks prior to
the exam, which counts 75%. Written report of a group assignment which counts
25%. Approval of written report is a prerequisite for participation in the exam.
Aid:
All Aid
Evaluation:
7 step scale , external examiner
Qualified Prerequisites:
27034/27416 , Fundamental knowledge on basic principles of fermentation
technology and biotechnological products produced by microorganisms.
Knowledge of the basic quantitative elements, the underlying science, and the
general concepts of biotechnological processes. Knowledge of cellular
metabolism. Optional prerequisites: 28020, 27611
General course objectives:
The aim of the course is to give the students a fundamental understanding of the interplay between the many
different intracellular reactions in a cell factory, and especially how the fluxes through the different pathways
are regulated. A special focus is given to pathways leading to industrially relevant products like primary
metabolites, antibiotics, industrial enzymes, and pharmaceutical proteins. A central aspect of the course is to
identify the optimal strategy for introducing directed genetic changes in the microorganisms with the aim of
obtaining better production strains. Analysis of the interaction between different cellular reactions is a central
element in the course, and tools for systems level strain characterization and design will be described.
Learning objectives:
A student who has met the objectives of the course will be able to:
Describe the synthesis and analysis elements of metabolic engineering and how they interact.
Describe the concepts behind metabolic flux analysis, and discuss advantages and disadvantages
with different methods.
Describe the principles of transcriptome, proteome and metabolome analysis and how data from
these analyses can be applied in cell factory engineering.
Describe how one constructs a genome-scale metabolic model.
Discuss the application of genome-scale models in design of optimal cell factories.
Design an experimental strategy for obtaining data to be used in cell factory design.
Design a cell factory optimization strategy based on metabolic knowledge, quantitative physiology and
omics data.
Write a scientific report and give an oral presentation on an real-life cell factory engineering example.
Content:
The course gives an overview of the different elements of cell factory design with a number of examples on
how directed genetic modification have been introduced with the aim of obtaining improved strains for
production of different compounds in the bioindustry. There is especially focus on the different tools of cell
factory design, and the course covers the following topics: Introduction to cell factory design. Overview of
biochemical pathways. Regulation of pathways. Examples of cell factory design. Design of experiments for
characterization of strains. Metabolic flux analysis: Theory and applications. Application of 13C-isotopes for
quantification of metabolic fluxes. Genomics. Whole genome transcription analysis. Proteome analysis.
Metabolome analysis. Methods for evaluation of omics data. Different case stories are used to illustrate the
topics. Students will work independently with examples and with a group task, which will be presented both
orally and in a written report. In the group task, students are introduced to a real-life cell factory design case
and are supposed to suggest innovative strain improvement strategies.
Remarks:
The course is aimed at biotechnology students, but the course can also be taken by students with a chemical
engineering background.
There is a chance to carry out strategies suggested in the group assignment in practice in the 3-weeks course
27432.
Green challenge participation:
Please contact the teacher for information on whether this course gives the student the opportunity to prepare
a project that may participate in DTUs Study Conference on sustainability, climate technology, and the
environment (GRN DYST). More inforhttp://www.groendyst.dtu.dk/english/Conference/Practical-info/Start-up
Responsible:
Mikael Rrdam Andersen , Building 223, room 224, Ph. (+45) 4525 2675 , mr@bio.dtu.dk






8233 Recovery and purification of biological products

Danish title:
Bioteknologiske oprensningsprocesser
Language:

English
Point( ECTS )

5
Course type:
MSc
Taught under open university

Technological Specialization Course, Biotechnology
Technological Specialization Course, Chemical and Biochemical Engineering
Technological Specialization Course, Food Technology



Schedule:
E2A (mon 13-17)


Course holdings

Location:
Campus Lyngby
Scope and form: Lectures and exercises
Duration of Course: 13 weeks

Date of examination:
E2A, Decide with teacher
Type of assessment: Written examination and reports
2 hours written examination without aids. Evaluation of 2
exercises. Each exercise counts for 20% of the total
course grade.
Exam duration: 2 hours
Aid:
No Aid
Evaluation:
7 step scale , internal examiner
Previous Course:
27406
Not applicable
together with:
27406
Qualified
Prerequisites:
Kemi- or Bioteknologifagpakke or bachelor or equivalent
General course objectives:
The objective of the course is to introduce the student to the downstream processing and purification of
biological products of relevance to the pharmaceutical, technical and food industries.
Learning objectives:
A student who has met the objectives of the course will be able to:
Arrange unit operations into an appropriate sequence for the purification of a given type of biological
product.
Clarify the interplay between upstream and downstream processes.
Argue the need for bio-intensification.
Explain the role that the necessary purity plays in the design of a downstream process.
Summarize scientific results from real examples and use them to choose the best operational
conditions for a particular unit operation.
Analyze scientific results from real examples and use them to choose the best operational conditions
for a particular operation.
Calculate operating parameters for a given downstream process unit operation
Use systematic design methodology for downstream processes
Understand use of hybrid and integrated processes
Content:
General introduction to industrial downstream processing. Work through of examples of industrial downstream
processing methods for different types of products. Principles of process design and development of
downstream processing strategies. Effect of fermentation development on downstream processing (upstream
versus downstream). Separation principles and their effectiveness. Product release, secretion, cell disruption,
flocculation processes, centrifugation, conventional filtration and membrane filtration, precipitation processes,
process chromatography, and product polishing. Case studies and developing the complete process.
Remarks:
The evaluated exercises will be done in groups of 2 and work done by each student must be identified, so that
each student can receive an individual grade.
Responsible:
John Woodley, Building 227, room 242, Ph. (+45) 4525 2885 , jw@kt.dtu.dk
Timothy John Hobley, Building 221, room 258, Ph. (+45) 4525 2706 , tjho@food.dtu.dk














28247 Advanced Enzyme Technology

Danish title:
Videregende enzymteknologi
Language:

English
Point( ECTS )

5
Course type:
MSc

Technological Specialization Course, Biotechnology
Technological Specialization Course, Chemical and Biochemical Engineering
Technological Specialization Course, Sustainable Energy



Schedule:
E1A (mon 8-12)


Course
holdings
Location:
Campus Lyngby
Scope and
form:
One weekly module with lectures, laboratory exercises, discussions, problem
solution exercises, and report work.
Duration of
Course:
13 weeks
Type of
assessment:
Evaluation of experiments and reports
Aid:
Written works of reference are permitted
Evaluation:
7 step scale , internal examiner
Previous
Course:
27507
Not applicable
together with:
27507
Mandatory
Prerequisites:
27506/ 28246, 28246 (previously 27506) Applied Enzyme Technology and
Kinetics is a required prerequisite for this course, but can be taken
simultaneously.
Qualified
Prerequisites:
27022. 27734. 28140, Bachelor in either: Chemistry and Technology;
Biotechnology; Food and Drug Technology; or Food Science or similar.
Participants
restrictions:
Minimum 10 Maximum: 24
General course objectives:
To introduce the students to enzyme kinetics in practice and research methods in applied
enzyme technology. The course is based on practical experimental methods and analyses within
applied biocatalysis and enzyme technology. After completion of the course, the course
participants will be able to:
Design, conduct and interpret various types of enzyme activity assays.
Examine and interpret quantitative, experimental data from biocatalytic reactions, and
understand the importance of considering blanks, substrate specificity, artificial vs.
complex/component substrates, heterogeneous enzyme preparations.
Plan and conduct experiments to estimate kinetic parameters and analyse progressing
biocatalytic reactions.
Design a biocatalytic process for a large scale application.
The academic qualifications obtained are targeted towards jobs research and development jobs
within biochemical, pharmaceutical and biotechnological companies as well as in academia.
Learning objectives:
A student who has met the objectives of the course will be able to:
Design experiments for the determination of enzyme kinetic parameters.
Conduct various types of enzyme activity assays on the basis of general methodological
descriptions.
Analyze results from these assays in order to estimate enzyme activity, enzyme stability,
thermal activation of enzymes, substrate saturation and inhibition constants.
Evaluate the validity of the enzyme kinetic results such i.e. considerations concerning
the use of blank samples, substrate specificity, use of artificial or multiple component
substrates and heterogenous enzyme preparations.
Analyze progressive enzyme reactions.
Critically read and evaluate scientific articles related to the subject area.
Work constructively in a team of two persons.
Report experimental results in a written form in a condensed scientific report structure:
Introduction, Methods, Results and Discussion, Conclusions.
Content:
The course is built around a number of specific themes that include research/theoretical study as
well as conduction of practical experiments that involve biocatalytic reactions. Emphasis is put
on unravelling the theory behind the reactions and on designing and conducting the practical
experiments. The course curriculum includes assessment of selected large scale processes in
which biocatalytic steps are involved. Complex processes that involve advanced exploitation of
biocatalysed conversions are also examined. The reactions to be covered relate to specific
scientific phenomena, selected industrial applications, and to current research topics. The
experimental work is carried out in teams of two, and each team will work with a selection of
cases (teams circulate among cases). In addition to the laboratory work, time is allocated for
discussing the scientific fundamentals, data interpretation, and for understanding the reaction
mechanisms: kinetics, substrate specificity, enzyme stability and synergism between different
enzyme activities. Each case must be summarized in a short report including answering of
specific questions relating to the particular case.
Green challenge participation:
Please contact the teacher for information on whether this course gives the student the
opportunity to prepare a project that may participate in DTUs Study Conference on
sustainability, climate technology, and the environment (GRN DYST). More
inforhttp://www.groendyst.dtu.dk/english/Conference/Practical-info/Start-up
Responsible:
Anne S. Meyer, Building 227, room 039/04, Ph. (+45) 4525 2909 , am@kt.dtu.dk

27432 Experimental Molecular Biology of Eukaryotes

Danish title:
Molekylr biologiske velser i eukaryoter
Language:

English
Point( ECTS )

5
Course type:
MSc
Taught under open university

Technological Specialization Course, Bioinformatics and Systems Biology
Technological Specialization Course, Biotechnology



Schedule:
January


Location:
Campus Lyngby
Scope and form: Exercises in the three-weeks period
Duration of
Course:
3 weeks

Date of
examination:
Decide with teacher
Type of
assessment:
Evaluation of exercises/reports
Evaluation of exercises/oral reports
Evaluation:
pass / not pass , internal examiner
Previous Course:
27232 and 27264
Not applicable
together with:
27232.27264
Qualified
Prerequisites:
27021/27025.27231/27025.27230.27430
Participants
restrictions:
Maximum: 24
General course objectives:
To provide the student with extensive practical experience in molecular biological, genetic and
physiological handling of eukaryotes.
Learning objectives:
A student who has met the objectives of the course will be able to:
Perform classic molecular biology/genetics experiments.
Describe DNA maintenance as well as chromosome segregation in eukaryotic cells. This
includes using fundamental molecular genetic terms.
Handle yeast and filamentous fungi.
Construct new strains by gene targeting.
Make point mutations, deletions and GFP tagging.
Plan and coordinate several experiments simultaneously.
Perform calculations based on the results derived from the various experiments
performed.
Analyze and interpret experimental results obtained from the various experiments
performed in the course.
Present and explain experimental results obtained during the course for an audience.
Discuss and evaluate results described in scientific articles in plenum
Content:
The laboratory course will focus on molecular biology, genetics and physiology of yeast and
filamentous fungi. During the course we will understand the dynamics of DNA. How often does it
mutate, how often does homologous recombination take place, how often do cells loose a
plasmid or a chromosome? Moreover, we will explore how defects in DNA damage repair
systems influence cellular fitness and behavior to provide specific clues to the actions DNA repair
mechanisms.
We will also look into how one can analyze a physiological relevant process by making specific
alterations of the genome of an organism. Hence, we will introduce point mutations, deletions
and GFP extensions in defined genes in the genomes of bakers yeast Saccharomyces cerevisiae
and the mold Aspergillus nidulans.
In the first part of the course we will transform and microbial cells for stain construction; and in
the second part, we will characterizef the resulting mutant strains and the introduced DNA
molecules. Classical genetic methods, DNA isolation, chromosome separation, restriction
analysis, tetrad dissection and analysis, replica plating, Southern blotting and hybridization and
PCR techniques are used to analyze the transformed strains and to understand the mechanisms
of genetic recombination operating in living organisms.
Remarks:
This course is recommended after course 27230 and 27430.
Green challenge participation:
Please contact the teacher for information on whether this course gives the student the
opportunity to prepare a project that may participate in DTUs Study Conference on
sustainability, climate technology, and the environment (GRN DYST). More
inforhttp://www.groendyst.dtu.dk/english/Conference/Practical-info/Start-up
Responsible:
Uffe Hasbro Mortensen, Building 223, room 230, Ph. (+45) 4525 2701 , um@bio.dtu.dk
Jakob Blsbjerg Nielsen, Building 223, room 218, Ph. (+45) 4525 2657 , jbn@bio.dtu.dk