The specimens (disc type; 10 x 2 mm) were placed on the bottom of a
24-well tissue culture plate containing liquid medium, and Candida albicans (CAD1) were cultured for 2 or 4 days in this plate. The optical densities (OD) of the bacterial culture were determined. The surface morphology of the mixtures was observed by scanning electron microscopy (SEM). Mechanical property: The dimensions of the cylindrical specimens created for mechanical tests were 10 mm 6 mm (diameter height). Flow measurement: ISO 10139-1 Compliance as measured by depth of penetration: ISO 10139-1 All results were analyzed using one-way ANOVA and a multiple comparison test. The SEM observation showed that the number of C.albicans on the mixtures had drastically decreased. Development of an antibacterial tissue conditioner with a micro-biologically derived component Naito Y 1 , Li Jing 1 , Hamada K 2 , Tomotake Y 1 , Nagao K 1 , Ichikawa T 1 1 Oral and Maxillofacial Prothodontics, 2 Biomaterials and Bioengineering, Institute of Health Biosciences University of Tokushima, Japan INTRODUCTION Tissue conditioners are materials used to promote healing of the damaged tissue that supports denture structures. However, many microorganisms such as Candida albicans are known to thrive on the surface of degraded tissue conditioners, and these often induce oral stomatitis and systemic diseases, such as aspiration pneumonia in compromised elderly people. Some researchers have attempted to synthesize antibacterial dental materials by using metals such as copper or silver, but these metals were sometimes found to induce metal allergy. OBJECTIVES In this study, a new antibacterial tissue conditioner by using Juncus was synthesized, and subsequently a microbiological and mechanical property were evaluated. CONCLUSIONS The tissue conditioners contain the Juncus powder has anti-microbiological properties, and are expect to prevent denture stomatitis and aspiration pneumonia in elderly people. EXPERIMENTALS RESULTS Materials Juncus powder (Inada, Yatsushiro, Japan) tissue conditioner (GC, Tokyo, Japan). Experimental procedures in this research Juncus powder was mixed at the concentration of 0, 2.5, 5, or 10 mass% with tissue conditioner powder. Anti-microbiological property (vs Candida albicans) 2 days 4days All mixtures tested after incubation showed significant anti-microbiological activity that was concentration-dependent. Flow measurement Compliance as measured by depth of penetration The mechanical properties were found to be within the regulated ranges specified by ISO 10139-1. Surface morphology of the specimens control 2.5 mass% 5 mass% 10 mass% In Japan, Juncus effusus is grown to be woven into the covering of tatami mats. Recently, this powder begins to be applied as food additives. p0.05 One-way ANOVA test and Tukeys HSD multiple comparison test
Comparative Evaluation of Antibacterial Efficacy and Effect On Dentin Microhardness of Triple Antibiotic Paste Modified With Nano Zinc Oxide and Nano Titanium Dioxide
International Journal of Innovative Science and Research Technology