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ADVANCED GCE
2815/02
CHEMISTRY
Biochemistry
*CUP/T76164*
Duration: 50 minutes
Scientific calculator
*
INSTRUCTIONS TO CANDIDATES
Write your name clearly in capital letters, your Centre Number and Candidate Number in the boxes above.
Use black ink. Pencil may be used for graphs and diagrams only.
Read each question carefully and make sure that you know what you have to do before starting your answer.
Answer all the questions.
Do not write in the bar codes.
Write your answer to each question in the space provided, however additional paper may be used if
necessary.
Max.
11
10
11
TOTAL
45
Mark
Turn over
2
Answer all the questions.
This question is about the structure of proteins. Five amino acids are shown below.
H
H2N
H
COOH
H2N
H
COOH
H2N
COOH
(CH2)2
CH3
NH2
phenylalanine
alanine
lysine
H
H2N
H
COOH
H2N
CH2
COOH
aspartic acid
COOH
CH2SH
cysteine
(a) Amino acids link together to form the primary structure of a protein.
(i)
Draw a diagram to show how alanine and lysine are linked in the primary structure of the
protein. Name the link involved.
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3
(b) In this question, one mark is available for the quality of use and organisation of scientific
terms.
Proteins have tertiary structure.
Using the amino acids opposite, describe three types of R group interactions that can help to
stabilise the tertiary structure of a protein.
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Quality of Written Communication [1]
[Total: 11]
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Turn over
4
2
H
COOH
H2N
COOH
tyrosine hydroxylase
HO
(a) (i)
OH
OH
tyrosine
L-DOPA
Suggest why the enzyme is specific for only one optical isomer of tyrosine.
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...................................................................................................................................... [1]
(ii)
[1]
(iii)
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(b) The compound 3-iodotyrosine is a competitive inhibitor of the enzyme tyrosine hydroxylase.
H
H2N
COOH
I
OH
3-iodotyrosine
(i)
(ii)
Draw graphs on the axes below to show how the initial rate of the enzyme-catalysed
reaction varies with tyrosine concentration:
initial rate
tyrosine concentration
[2]
[Total: 6]
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Turn over
6
3
GATACG
GATACG
...............
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[1]
7
(d) Translation uses m-RNA to direct the synthesis of a protein chain.
Describe the process of translation.
Your answer should include details of the bond-breaking, bond-making and intermolecular
forces involved.
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[Total: 10]
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Turn over
8
4
H
C OH H C
HO
OH
OH
- .......................................................................................................................................
pyranose ....................................................................................................................... [2]
(ii)
[1]
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(b) A disaccharide can be formed from two D-glucose molecules connected by a 14 glycosidic
link.
(i)
[2]
(ii)
(ii)
Apart from solubility, explain how the bonding and structure of cellulose makes it suitable
as a structural polymer for plants.
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[Total: 11]
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Turn over
10
5
Punicic acid, C17H29COOH, is the main carboxylic acid found in pomegranate seeds. It is an
unsaturated straight chain compound.
(a) Deduce the number of carbon to carbon double bonds in punicic acid.
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(b) A triglyceride can be made from punicic acid and glycerol (propane-1,2,3-triol).
(i)
[2]
(ii)
(iii)
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BLANK PAGE
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Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every
reasonable effort has been made by the publisher (OCR) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.
OCR is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of University of Cambridge Local Examinations Syndicate (UCLES),
which is itself a department of the University of Cambridge.
OCR 2009