Supplemental Table 3 related to figure 4.

Lineage tracing
gene translational pattern
die-1 ubiquitous and germline expressed; degraded at morphogenesis
lin-13 ubiquitous and germline expressed; subnuclear foci
sdc-2 ubiquitous and germline expressed; becoming focal within nucleus
hlh-1 muscle precursors
pha-4 pharyngeal, intestinal and hindgut cells
ceh-16 lateral row of cells
ceh-27 subset of pharyngeal and neuronal precursors
cnd-1 lateral neuronal precursors
egl-5 C lineage muscle subset
elt-2 intestinal precursors
elt-3 hypodermal precursors
glp-1 highly patterned, primarily membrane-localized
lin-12 highly patterned, primarily membrane-localized
lin-39 lateral blast cell and C lineage subset
med-2 EMS progeny
nhr-2 broadly expressed (most cells) from 24-100 cell stage, then degraded
sea-1 Patterned in early embryos, then becoming ubiquitous
tbx-8 highly patterned
unc-130 head cells
transcriptional pattern Match Additional information
ubiquitous; not germline expressed yes + germ regulated degradation
ubiquitous in embryo, not germline expressed yes + germ subcellular localization
ubiquitous; not germline expressed yes + germ subcellular localization
muscle precursors and other MS cells yes increased specificity of pattern
pharyngeal, intestinal and hindgut cells yes regulated degradation
lateral row of cells yes
subset of pharyngeal and neuronal precursors yes
lateral neuronal precursors yes regulated degradation
C lineage muscle subset yes
intestinal precursors yes onset time
hypodermal precursors yes
highly patterned yes subcellular localization
highly patterned yes subcellular localization
lateral blast cell and C lineage subset yes
EMS progeny yes degradation
ubiquitous yes
Ubiquitous with quantitative patterning yes
highly patterned yes
head cells yes
gene strain
Genes with movies and traced lineages
alr-1 RW10757
ama-1 RW10424
ceh-14 RW10726
ceh-26 RW10913
ceh-6 RW10871
cnd-1 RW10434
cnd-1 RW10435
die-1 RW10376
die-1 RW11053
egl-5 RW10600
egl-5 RW10867
elt-2 RW10714
eor-1 RW10868
fkh-4 RW10914
hlh-1 RW10481
hnd-1 RW10196
lin-11 RW10493
lin-13 RW10560
lin-39 RW10349
lir-2 RW11163
mab-5 RW10350
med-2 RW10479
mep-1 RW11050
nhr-2 RW11045
nhr-23 RW11070
nhr-25 RW10231
nhr-25 RW10348
pal-1 RW10889
pal-1 RW10993
pha-4 RW10425
tbx-8 RW10558
ttx-3 RW10713
unc-130 RW10866
unc-130 RW11144
Expression summary
Genes with movies and traced lineages
L-R Asymmetric in C and MS, expressedin GLR precursors and several neurogenic and hypodermal AB
Ubiquitous
No expression by 350 cell stage; expressing cells at comma identified as VR, PVT, DVC, one
pharyngeal gland cell and AFD neurons
Excretory cell and RMEV
Several AB lineages including rectal and excretory precursors, SMBV and dorsal motor neurons
Dynamic in AB neurogenic lineages, persistent in subset of DD motorneurons
Dynamic in AB neurogenic lineages, persistent in subset of DD motorneurons
Weak maternal expression becoming bright around 200 cell stage and then lost except in subset of
head neurons, hypodermal and pharyngeal cells
Weak maternal expression becoming bright around 200 cell stage and then lost except in subset of
head neurons, hypodermal and pharyngeal cells
Posterior C muscle, rectal cell U
Posterior C muscle
E lineage
Ubiquitous with brighter expression in some neuronal lineages
Ubiquitous for short temporal window at 28-50 cell stage
Most muscle precursors
Most muscle precursors
No expression by 350 cell stage; expressed by comma in several likely neurogenic cells
Ubiquitous, with bright subnuclear foci in some cells
Medial to anterior P cells, seam cells, C hypodermal and C muscle cells
Ubiquitous
Medial to posterior P cells, seam cells, C hypodermal and C muscle cells
Weak in E, MS
Ubiquitous, with bright subnuclear foci in some cells
In most or all cells for a short temporal window peaking at the 100-200 cell stage
Many hypodermal lineages
Most hypodermal lineages
Most hypodermal lineages
Maternal, retained and strengthened in C and D lineages, later expressed in posterior hypodermis and
mesodermal cells
Maternal, retained and strengthened in C and D lineages, later expressed in posterior hypodermis and
mesodermal cells
Expressed in all pharyngeal precursors and more weakly in intestinal and rectal precursors
Expressed in ABa-derived neuronal and hypodermal lineages, MS muscle, C hypodermis and posterior
Expressed after 350 cells in neurons including AINL, ILshL, ASKL, ASKR, AIYL, AIYR,SMDDL
Expressed in a subset of D muscle, C hypodermis, intestine, and ABp-derived hypodermis and neurons
Expressed in a subset of ABp-derived hypodermis and neurons
protein CND-1 DIE-1
correlation coefficient 0.85 0.66
replication fraction 0.98 1
LIN-12/Notch nuclear GFP fluorescence compared to sister lineages without signaling
sister lineage pairs with/without known Notch
signaling
Eel/Ear ABplpap/ABprpap
significance of the fluorescence intensity
differences
2.87E-05 4.77E-05
Reproducibility between different types of reporters
reporter types protein/protein promoter/promoter
reproducibility (mean correlation coefficient) 0.79 0.85
replication fraction 0.94 0.99
number of strains 13 92
Reproducibility between independent strains for the same protein
EGL-5 PAL-1 UNC-130
0.8 0.89 0.91
0.98 1 0.93
LIN-12/Notch nuclear GFP fluorescence compared to sister lineages without signaling
ABplaaa/ABpraaa
0.036309133
Reproducibility between different types of reporters
protein/promoter
0.54
0.62
12
Reproducibility between independent strains for the same protein