Department of Medical Microbiology University of Nairobi Learning Objectives At the end of this lesson, the learner should be able to:
Classify the parasite Enterobius vermicularis Outline geographical distribution of E. vermicularis Describe morphology of key stages of E. vermicularis Describe the life cycle of E. vermicularis Describe clinical manifestations of E. vermicularis Describe the tests used to diagnose E. vermicularis Outline treatment of E. vermicularis Describe control of E. vermicularis 1.0 Introduction Intestinal nematode, found in the large intestines Also known as pinworm Worldwide distribution, more in temperate climates More common in overcrowding places Orphanages, boarding schools etc Children most affected high prevalence & intensity Cause disease known as enterobiasis 2.0 Morphology Adults Females 9-12 mm, Males 2-3 mm Thick lips (cervical alae) Eggs Planocovex in shape Transparent wall Embryonated
Adult, Enterobius vermucularis
Egg in Iodine stained preparation Egg in wet saline preparation 3.0 Life cycle Adults mate in LI, females get out of anus, lay eggs Eggs passed embryonate within a few hours Itchiness of perineum, scratching, stick to fingers & nails, ingested accidentally (self or others), reach SI, hatch, larvae move to LI, attach to mucosa, adults May be transmitted through clothing, beddings, food etc contaminated with eggs, dustborne by inhalation, retroinfection Life cycle 4-6 weeks
4.0 Clinical presentation Light infection mostly asymptomatic Clinical presentation includes Pruritus ani (scratching the anus) usually at night Sleep disturbance Irritability & lack of concentration in school Poor class performance Loss of appetite Complications include Appendicitis Salpingitis in females (rare) 5.0 Laboratory Diagnosis Microscopic identification of eggs must be done in the morning, before defecation and washing by Adhesive scotch tape test - Press transparent adhesive tape on the perianal skin & then examining tape placed on a slide Anal swabs Press a swab coated with adhesive material on the perianal skin Camel hair brush Eggs occasionally found in stool, urine, vaginal smears Adult worms may also be found in perianal area
Enterobius vermicularis eggs in a cellophane tape preparation 6.0 Treatment Mebendazole Albendazole Pyrantel Pamoate * In institutional outbreaks treat all the household members 7.0 Prevention & Control Proper hygiene Daily bathing Frequent changing of clothes & beddings Keeping short nails etc, Avoiding overcrowding, opening of windows for ventilation etc Health education Washing of hands before eating of a meal Treatment of infected persons with antihelminthes