XBI2M012

UNIVERSITY OF DUBLIN
TRINITY COLLEGE

Health Sciences

BIOCHEMISTRY

Dr. Richard K. Porter



1
st
Medical Year Michaelmas Term 2008

FIRST BIOCHEMISTRY ASSESSMENT


Multiple Choice Questionnaire

Tuesday 9 December 9.30am 10.30am Luce Hall



Answer all 20 questions
Only ONE of the five options is correct
There is NO negative marking
Indicate your student number and answers on the Optical Reader sheet
IN PENCIL ONLY






Items required for this examination: student ID card, pen(s),
pencil(s), eraser, ruler.

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XBI2M012
Page 2 of 6

1. Galactosaemia (galactosemia) results, predominantly, from the lack of
activity of which one of the following enzymes:

(a) galactose-1-phosphate uridyl transferase
(b) galactokinase
(c) UDP-galactose-4-epimersae
(d) Phosphofructokinase
(e) Lactate dehydrogenase

2. Which one of the following cycles is known as the Cori cycle:

(a) the substrate cycle between the enzymes of phosphofructokinase and
fructose 1,6-bisphosphatase
(b) the conversion of glucose to lactate in muscle and the transfer of lactate
from muscle in the bloodstream to the liver for conversion to glucose
(c) the tricarboxylate cycle
(d) the urea cycle
(e) the pentose phosphate pathway

3. Which one of the following biochemicals is NOT a product of the pentose
phosphate pathway?

(a) NADH
2

(b) NADPH
2

(c) ribose-5-phosphate
(d) ribulose-5-phosphate
(e) fructose-6-phosphate

4. Which one of the following members of the glucose transporter family is
the insulin-sensitive glucose transporter:

(a) glut 1
(b) glut 2
(c) glut 3
(d) glut 4
(e) the sodium-dependent glucose transporter

5. Glucose-6-phosphatase is a key enzyme in glucose production by the liver
and kidney and is found in which subcellular location:

(a) the mitochondria
(b) the cytosol
(c) the peroxisome
(d) the endoplasmic reticulum
(e) the ribosome
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XBI2M012
Page 3 of 6

6. How many carbon dioxide molecules are produced by one round of the
Krebs cycle?

(a) one
(b) two
(c) three
(d) four
(e) six

7. McArdles Glycogen Storage Disease, which limits ability to perform
exercise due to painful cramps, results from a defect in which one of the
following enzymes:

(a) amylase
(b) glycogen phosphorylase
(c) glycogen synthase
(d) hexokinase
(e) creatine kinase

8. How many carbons are there in oxaloacetate?

(a) three
(b) six
(c) two
(d) four
(e) five


9. The Km of an enzyme catalysed reaction is

(a) the substrate concentration that gives half the maximal rate (Vmax)
(b) the substrate concentration that gives one quarter of Vmax
(c) not related to the concentration of substrate
(d) is alway 1mM
(e) is aways 10mM


10. The Vmax of an enzyme-catalysed reaction is

(a) defined as Km*S
(b) not dependent on the total enzyme concentration (Et)
(c) dependent on the total enzyme concentration (Et)
(d) defined as Vmax/Km
(e) always 10 micromol/min/mg

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XBI2M012
Page 4 of 6

11. The Michaelis-Menten equation is vo =

(a) kcatEt
(b) Vmax/Km
(c) Vmax[S]/(Km + [S])
(d) [S]/(Vmax + [S])
(e) Et*Et

12. The allosteric site in an allosteric enzyme is

(a) the catalytic site
(b) an "other" site
(c) a virtual site
(d) a building site
(e) not formed during the catalytic cycle

13. Protein kinases regulate the activity of their target protein by

(a) methylation
(b) adenylation
(c) sulphation
(d) deamidation
(e) phosphorylation


14. Lactate dehydrogenase catalyses the

(a) hydrolysis of para-nitrophenylsulphate
(b) hyrolysis of para-nitrophenylphosphate
(c) hyrolysis of casein
(d) NAD+ dependent oxidation of lactate
(e) hydrolysis of lactose





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XBI2M012
Page 5 of 6

15. Of the following four statements on amino acids, peptides,
and proteins, how many are true statements?

Only at very low or very high pH does the non-ionized form
of an amino acid predominate.
Leu is more non-polar than Ala.
At a pH greater than the pK
a
of an ionizable group, more
than half of such groups are dissociated.
Ion-exchange chromatography separates amino acids and
proteins on the basis of their sizes.

(a) None
(b) One
(c) Two
(d) Three
(e) Four (= all)

16. Of the following four statements on proteins, how many
are true statements?

The thermodynamically most stable conformation of a
protein is the structure of lowest free energy.
The term quaternary structure refers to protein
configuration in the fourth dimension, as a function of time.
The amide hydrogen of every peptide bond in an -helix is
H-bonded.
The side chains of amino acid residues point to the outside in an
-helix.

(a) None
(b) One
(c) Two
(d) Three
(e) Four (= all)


17. Which grouping of amino acid residues would you expect to find on the
inside (interior) of a typical globular protein molecule in solution at
pH = 7?

(a) Glu, Phe, Met
(b) Ile, Lys, Ser
(c) Asn, Thr, Phe
(d) Val, Phe, Ile
(e) Ser, Thr, Glu

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XBI2M012


Page 6 of 6


18. The amino acid composition analysis of a small tripeptide resulted in the
recovery of equimolar quantities of phenylalanine (Phe), valine (Val), and
lysine (Lys). With the knowledge that this is a tripeptide, how many
different peptides can be formed from this resulting composition?

(a) Three (3)
(b) Six (6)
(c) Nine (9)
(d) Twenty-seven (27)
(e) Cannot be estimated with the given information


19. What is the isoelectric pH (pI) for the amino acid tyrosine (Tyr), which
has a pK
1
of 2.2, a pK
2
of 9.1, and a pK
R
of 10.1?

(a) 5.65
(b) 6.15
(c) 7.13
(d) 9.6
(e) Cannot be estimated with the given information.

20. In regard to the T (taut) and R (relaxed) states of hemoglobin, which one
of the five options (a to e) given below, (correctly) describes the
following three statements:
i) this state binds oxygen more easily?
ii) this state has more electrostatic interactions?
iii) this state has the larger opening for oxygen to diffuse into the
hemes?

(a) i) T ii) R iii) R
(b) i) T ii) T iii) R
(c) i) R ii) T iii) T
(d) i) R ii) T iii) R
(e) i) R ii) R iii) R





University of Dublin 2008