Discussion.

First question
The table provides information about test food experiment on five types of substances.
substance procedure The table above
provides information how food
test were done and what changes
occurred.


observation result
Starch 2ml starch+2 drops of Iodine Deep blue-black colour +
Mono-
disaccharides
2 ml sugar+100ml benedict
reagent(if non-reducing sugar for
example sucrose 3 drops of HCL,
NACO3 )
Red-brown color +
protein KOH+CuSO4.it is called Biuret test Purple color appears +
Lipid1 1 drop of oil+2ml ethanol +large
quantity of water
White milky solution +
Lipid2 Oil +water two drop of Sudan 3
dye
Lipid colored by sudan in red +
Vitamin C 2ml DCPIP + ascorbic acid DCPIP lost color +


Second question.
The test for sugars was done in two stages. First stage is when Benedict reagent is added to to sugar the
reduction of Cu(II ) to Cu(i) happens. In order to complete reaction the second stage was undertaken ,
so the solution was heated at the water bath. The red color appeared, because precipitation Copper
oxide turn to this color. Reducing sugars like fructose, glucose and maltose had positive result, because
they contain in their structure aldehyde or ketone groups. However, the sucrose solution did not
showed positive result, because it is non-reducing sugar. So in order to break glycosidic bonds HCL was
added and in order to neutralize the solution sodium carbonate was also added. So after this procedure
the sucrose divided into glucose and frucrose. Then the solution showed positive result.

Third question.
This table shows the test on the unknown solutions.
Number oftest
tubes


Test for
substances

For Starch

For Protein

For Sugar

For Vitamin C

For Lipid

1
st
tube

No expected
result

No expected
result

Positive result

No expected
result

No expected
result

2
nd
tube

No expected
result

No expected
result

Positive result

Positive result

No expected
result

3
rd
tube

No expected
result

Positive result

Positive result

No expected
result


No expected
result

The test examined three unknown substances. Every substance was observed by 5 food tests. When
expected result was seen it means that this solution contain this type of biomolecules. As it is shown in
the table the substance from first tube not contain any protein, starch, vitamin C and lipid molecules,
but when the test for presence of sugar was undertaken the color of this solution changed to orange.
This means that sugar might present in this solution. The same procedure was done with second and
third unknown solutions. And during the test the second solution gave also positive result for sugar test
and this means that it also contain sugar molecule. Interesting observation occurred with third solution ,
because it contained two types of molecules. The appearance of purple color shows that it contain
protein and orange color indicates that there are sugar molecules present. This ends my report.

















Lab Practical #6.
Food test.



















Kaparova Zhamilya.
Group D.
Andy Hawkins.
Nazarbayev University.(23.11.2012)