Title: Cellular Respiration-Heat Energy Release in

Respiration
Materials: 250 grams mongo seeds
2 Beaker
2 Thermo Flask
2 Small Vials
2 Cork Stopper
2 Thermometer
Objectives:
1. To demonstrate how heat is released during
respiration.
2. To understand how and why heat energy is
released in respiration.
Procedure:















Results:
Temperature Reading
(C)
Boiled
Seedlings
Unboiled
Seedlings
Initial Temperature 27 C 27C
15 min 31C 27C
30 min 29C 28C
45 min 27C 28C
60 min 24C 29C
75 min 23C 30C
90 min 22C 31C




(Boiling of seedlings) (Thermo Flasks set up)
Explanation:
The temperature of the boiled seedlings was
higher compared to the unboiled seedlings on the first
15minutes because of the heat absorbed by the seeds
when boiled. After a period, the temperature of the
boiled seeds lowered while the temperature of the
unboiled seeds continuously increases.
The purpose of sterilizing the boiled seedlings
with formalin is to kill or suppress other factors such as
bacteria and other microorganisms that will affect the
setup. Formalin is used to kill bacteria and
microorganisms which are present in decomposition of
materials.
Conclusion:
The seedlings respire aerobically because of the
presence of oxygen that is trapped inside the flasks. In
addition, germinating seedlings release heat while non-
germinating seedlings do not. Lastly, we have observed
that respiration could not take place in boiled seeds
therefore there is no heat produced.
References:
1. Vasquez, Laurente, Cobar; Laboratory Manual
For Pharmaceutical Botany
2. J. Mauseth; Botany: An Introduction to Plant
Biology
3. http://www.scribd.com/doc/157841058/Heat-
Energy-Released-in-Respiration







1D-PH Group 6 Members:
QUEZADA, Esther Florentina
RARIZA, Mary Chelsea
RIFAREAL, Katrina Isabel
RODRIGUEZ, Alecs Dale
SAN PEDRO, Blessing

Soak 250 g mongo seeds
overnight
Boil half of the seedlings for 5 mins
Decant water and allow it to cool
Add 100 ml formalin solution
for 5 mins then remove
Place it inside the thermo flask
Suspend a small vial w/ KOH w/ a cork stopper and
thermometer
Place half of the seedlings in
the thermo flask
Record the initial temperature and temperature
every 15 mins for 2 hour interval