PromoCellKatalog2013 Lowres

PromoCell GmbH
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2013 | 2014
Cell Culture & Cell Biology
2013 | 2014
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Human Primary Cells & Media

Stem and Blood Cells & Media
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PromoCell catalog
PromoCell
PromoKine catalog
PromoKine
PromoCell Academy catalog
PromoCell Academy
Date, Signature
Overview
About PromoCell
Human Primary Cells
Human Stem and Blood Cells
Media and Sera
Cell Pellets
Reagents and Supplements
Cell Analysis
Apoptosis
Fluorescent Labeling
Cell Transfection
10
Antibodies and ELISAs
11
Cytokines and Growth Factors
12
Microbial Detection and Elimination
Contents
Overview
Contents
Contents
Contents
0
1
About PromoCell
PromoCell Ethical Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
PromoCells Knowledge Base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Human Primary Cells

1.1 Cardiac Myocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.2 Chondrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.3 Endothelial Cells (Large Vessels) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.4 Endothelial Cells (Microvascular) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.5 Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.6 Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.7 Follicle Dermal Papilla Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.8 Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.9 Keratinocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.10 Melanocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.11 Osteoblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.12 Preadipocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.13 Skeletal Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.14 Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
11
12
13
17
23
27
30
31
32
33
34
35
36
37
Overview Human Primary Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Human Primary Cells by Tissue Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

2.1 Mesenchymal Stem Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.2 Pericytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.3 CD34+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.4 CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.5 Monocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.6 Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
49
51
52
53
54
56
Overview Human Stem and Blood Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
3
4
Media and Sera

3.1 Media for Primary Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.2 Media for Stem and Blood Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.3 Classical Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.4 Insect Cell Culture Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.5 Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
61
80
85
87
87
Cell Pellets
4.1 Cell Pellets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Contents
6
7
8
9

5.1 Detachment Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.2 Antibiotics and Antimycotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.3 Buffers and Salt Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.4 Supplements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
95
97
98
99
Cell Analysis
6.1 Cell Viability and Proliferation Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.2 Live/Dead Cell Staining Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.3 Cytotoxicity Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.4 Senescence Detection Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.5 Cell-Based Reporter Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.6 Cell Staining Reagents and Related Products . . . . . . . . . . . . . . . . . . . . . . .
6.7 Intracellular Indicators and Related Products . . . . . . . . . . . . . . . . . . . . . . . .
6.8 Cell Signaling Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.9 Cell Stress and Metabolism Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.10 Cell Fractionation and Protein Extraction Kits . . . . . . . . . . . . . . . . . . . . . . .
6.11 Protein Size Markers and Protein Gel Stains . . . . . . . . . . . . . . . . . . . . . . . .
6.12 Nucleic Acid Isolation Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.13 DNA Size Markers and DNA Gel Stains . . . . . . . . . . . . . . . . . . . . . . . . . . . .
103
105
106
106
107
109
115
119
120
122
123
124
125
Apoptosis
7.1 DNA Fragmentation Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.2 Mitochondrial Apoptosis Staining Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.3 Cytochrome c Apoptosis Detection Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.4 Glutathione Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.5 Annexin V Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.6 Caspase Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.7 Cathepsin/Calpain Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.8 Kinase Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.9 Apoptotic and Necrotic Cell Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.10 Apoptotic Cell Isolation Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.11 Apoptosis Inducers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
129
130
130
131
132
134
138
139
140
141
141
8.1
8.2
8.3
8.4
8.5
PromoFluor Dyes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PromoFluor Labeling Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PromoFluor Labeled Biomolecules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Phycobiliprotein Dyes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PromoFluor Antifade Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
145
148
150
152
153
Cell Transfection
9.1
9.2
9.3
9.4
PromoFectin Transfection Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Magnet Assisted Transfection (MATra) . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Transfection Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Reporter, Expression and Fusion Vectors . . . . . . . . . . . . . . . . . . . . . . . . . . .
157
161
165
166
Contents
Contents
Contents
Contents
10
11
10.1 Mono- and Polyclonal Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

10.2 Antigens and Cell Lysates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
10.3 Labeled Antibodies and Labeling Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
10.4 ELISA Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
169
172
173
175
11.1 Cytokines, Growth Factors, Hormones, and soluble Receptors . . . . . . . . . . . 179

11.2 Animal-free Cytokines and Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . 180
11.3 Cytokines and Growth Factors for Stem Cell Research . . . . . . . . . . . . . . . . 181
12
13
Appendix
12.1 Mycoplasma Test Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

12.2 Mycoplasma Elimination Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
12.3 Bacteria Test Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
12.4 Disinfectants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Alphabetical Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
International Distributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Terms and Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Ordering and Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fax Order Form . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
185
186
188
189
191
200
203
205
209
About PromoCell
Over 20 years ago PromoCell started its business in the
so-called City of Science: Heidelberg. Heidelberg is not only
home to the oldest university in Germany but is also popular for
research related businesses. The city provides the ideal scientific
environment for PromoCell and serves as initial point for all our
operations.
0 About PromoCell
PromoCell Ethical Standards

Ethical and legal standards
governing Primary Human Cells
About PromoCell
PromoCell is the original manufacturer

of all primary, stem, and blood cells featured in this catalog and is committed to
the highest ethical and legal standards.

Consequently, PromoCell strictly complies with the following procedure for
the donation and collection of human
tissue used for cell isolation:
Extract from The Convention of Human Rights and Biomedicine:

Article 5 General rule
An intervention in the health field may only be carried out after the person
concerned has given free and informed consent to it.
This person shall beforehand be given appropriate information as to the purpose and nature of the intervention as well as on its consequences and risks.
The person concerned may freely withdraw consent at any time.
Article 22 Disposal of a removed part of the human body
When in the course of an intervention any part of a human body is removed,
it may be stored and used for a purpose other than that for which it was
removed, only if this is done in conformity with appropriate information and
consent procedures.
The tissue used by PromoCell for the

isolation of human cell cultures is derived from donors who have signed
an informed consent form (this being
done by the donor himself, an authorized agent, or a legal agent) which
outlines in detail the purpose of the
donation and the procedure for processing the tissue. We do not accept
or use any tissue without prior signing
of the consent documents.
When obtaining and using human
tissue, PromoCell acts in strict compliance with
1. The Convention for Protection
of Human Rights and Dignity of the
Human Being with Regard to the Application of Biology and Medicine:
Convention of Human Rights and
Biomedicine published on April 4th,
1997 by the Council of Europe (European Treaty Series no 164).
2. The Human Tissue Act published
on November 15th, 2004 by the government of the United Kingdom. This
act aims to make consent a fundamental principle underpinning the use
and storage of human tissue.
Extract from The Declaration of Helsinki:

Article 22
In any research on human beings, each potential subject must be adequately
informed of the aims, methods, sources of funding, any possible conflicts of
interest, institutional affiliations of the researcher, the anticipated benefits
and potential risks of the study and the discomfort it may entail. The subject
should be informed of the right to abstain from participation in the study or
to withdraw consent to participate at any time without reprisal. After ensuring
that the subject has understood the information, the physician should then
obtain the subjects freely-given informed consent, preferably in writing. If
the consent cannot be obtained in writing, the non-written consent must be
formally documented and witnessed.
3. The Declaration of Helsinki developed by the World Medical Association as a statement of ethical principles to provide guidance to physicians
and other participants in medical
research involving human subjects.
4. The German Federal Data Protection Act to protect privacy of donors.
Should you have any questions concerning ethical aspects related to the
use of Primary Human Cells, do not
hesitate to contact us or visit our
website:
www.promocell.com/ethics
About PromoCell
0 About PromoCell
At PromoCell we continuously work to improve our products and services.
Dear Customer,
Henry Ford once said: Coming together is a beginning. Keeping
together is progress. Working together is success. At PromoCell
we are proud to work together successfully with scientists worldwide, providing them with high quality products and experienced
technical customer support. With over 5,000 PromoCell and
PromoKine products we have the tools to meet the needs of your
research project!
Under our PromoCell brand we offer a broad range of human
primary cells, stem cells and blood cells, as well as optimized cell
culture media. Scientists worldwide use PromoCell products in basic and applied biomedical research to obtain better results from
more accurate physiological models. The reliability of our cells provides the basis for being able to offer you the high quality standards you need.
For more in depth analysis of your cells in culture, why not look to
our PromoKine brand? PromoKine offers quality kits and reagents
for cell biology including those for cell analysis, cell transfection,
and fluorescent labeling and also provides numerous antibodies,
ELISAs, cytokines and growth factors.
In order to complete our portfolio we established the PromoCell
Academy in 2004. The PromoCell Academy offers courses with
state-of-the-art insight and up to date trends in the Life Sciences
in a professional and hands-on setting. You can choose from a
growing collection of course topics in German as well as in English.
We are looking forward to working with you and providing you
with our support!
Scientists supporting Scientists guaranteed.
Your PromoCell Team
0 About PromoCell
About PromoCell
Discover our Knowledge Base
Need Information?
Information at your fingertips
Sections of the Knowledge Base include:
As scientists, we know that research is

not a nine-to-five job. Scientists need information fast, at any time, day or night.
Technical
To assist you with this, PromoCell has

created our new online Knowledge Base.
At the touch of a button, you can now
find a wealth of product-related and
technical information to support your
research needs.
You can find detailed answers to many

questions about our products in the section Technical Library. Type in a keyword and the Knowledge Base will show
you the associated technical questions
and answers within seconds.
Library
Notes
Reference Literatures
Application
In the section Application Notes you

will find documents which supplement
the information in our Product Manuals
(e.g. detailed differentiation protocols
and staining procedures).
If you are interested in viewing publications by our customers, the section
Reference Literature offers you access
to literary citations of scientists that use
our PromoCell and PromoKine products.
To access the PromoCell Knowledge Base, please

go to: www.promocell.com/knowledge-base
To access the PromoKine Knowledge Base, please

go to: www.promokine.info/knowledge-base
We appreciate your feedback

We are continually improving our products and services to make them fit your
needs. Your feedback, ideas and suggestions therefore are valued and important
to us.
For your convenience we have created
a feedback form online for this purpose:
www.promocell.com/feedback
We thank you in advance for providing

us with your ideas and suggestions.
Of course our customer support team
is also happy to receive your feedback
via email, fax or telephone.
To find your personal area contact,
please refer to the contact section on our
website or see page 205.
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db
e
Fe
www.promocell.com/feedback
Human Primary Cells

Experiments with human primary cells simulate in vivo conditions much better and yield more significant results than those
using immortalized cell lines. This is because the primary cells
genome and expression patterns have not been altered by
immortalization and numerous passages in culture.
PromoCell offers a wide variety of high quality human primary
cells from tissue obtained from approved medical centers.
10 Content Chapter 1
Human Primary Cells

1.1
Cardiac Myocytes . . . . . . . . . . . . . . . . . . . . 11
1.2 Chondrocytes . . . . . . . . . . . . . . . . . . . . . . . 12
1.3
Endothelial Cells (Large Vessels) . . . . . . . . . 13
Umbilical Vein . . . . . . . . . . . . . . . . . . . . . . 13
Umbilical Artery . . . . . . . . . . . . . . . . . . . . . 14
Aortic / Coronary Artery . . . . . . . . . . . . . . . 15
Pulmonary Artery / Saphenous Vein . . . . . . 16
1.4
Endothelial Cells (Microvascular) . . . . . . . . 17
Dermal . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Dermal Blood . . . . . . . . . . . . . . . . . . . . . . . 18
Dermal Lymphatic . . . . . . . . . . . . . . . . . . . 19
Bladder / Cardiac . . . . . . . . . . . . . . . . . . . . 20
Pulmonary / Uterine . . . . . . . . . . . . . . . . . 21
Brain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
1.5
Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . 23
Nasal / Tracheal . . . . . . . . . . . . . . . . . . . . . 23
Bronchial / Small Airway . . . . . . . . . . . . . . . 24
Mammary / Renal . . . . . . . . . . . . . . . . . . . 25
Renal Cortical / Placental . . . . . . . . . . . . . . 26

1.6 Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . 27
Dermal . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Pulmonary / Aortic Adventitial / Cardiac . . 28
Uterine / Villous Mesenchymal . . . . . . . . . . 29
1.7
Follicle Dermal Papilla Cells . . . . . . . . . . . . . 30
1.8 Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . 31
1.9 Keratinocytes . . . . . . . . . . . . . . . . . . . . . . . 32
1.10 Melanocytes . . . . . . . . . . . . . . . . . . . . . . . . 33
1.11 Osteoblasts . . . . . . . . . . . . . . . . . . . . . . . . 34
1.12 Preadipocytes . . . . . . . . . . . . . . . . . . . . . . . 35
1.13 Skeletal Muscle Cells . . . . . . . . . . . . . . . . . 36
1.14 Smooth Muscle Cells . . . . . . . . . . . . . . . . . 37
Aortic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Coronary Artery / Pulmonary Artery . . . . . . 38
Umbilical Artery / Tracheal . . . . . . . . . . . . . 39
Bronchial / Bladder . . . . . . . . . . . . . . . . . . . 40
Prostate / Uterine . . . . . . . . . . . . . . . . . . . . 41
Overview Human Primary Cells . . . . . . . . . . . . . . 42

Human Primary Cells by Tissue Type . . . . . . . . . . . 45
1 Human Primary Cells
11
Human Primary Cells

PromoCell offers a wide variety of high
quality human primary cells. The tissue
used for the isolation of these cells is
obtained from approved medical centers following strict ethical standards
(see page 6 for details). Shortly after
isolation, the cells are cryopreserved
using the unique serum-free PromoCell
Cryo-SFM (see page 79) and an op-
timized, computer controlled freezing

profile. This allows high viability of the
cells and defined culture conditions after
thawing.
All PromoCell primary cells are available
cryopreserved or proliferating. Each cell
lot is tested for cell type specific markers,
cell morphology, population doubling
time, and proliferation capacity. In addi-
tion, tests for the absence of HIV 1, HIV 2,

HBV, HCV, fungi, mycoplasma, and other
bacteria are performed.
Moreover, cell pellets prepared from released cell lots and stored in RNAlater
are available. They are useful for DNA,
RNA, and protein isolation and their subsequent analysis (for more information
see page 91).
Applications
Cardiac
research
studies
Pharmacological studies
Physiological
Features
Adult
origin
-actinin positive
Slow muscle myosin positive
Qualified for long-term experiments
Sarcomeric
Human Cardiac Myocytes (HCM) are

isolated from the ventricles of the adult
heart. They are qualified for in vitro
research on cardiac diseases and for
pharmacological studies. Unlike freshly
isolated rod-shaped myocytes, cultured
HCM can be used for long-term experiments like investigating the long-term
effects of cytokines, mechanical strain,

or cell-cell interactions, as they are prepared according to a special protocol.
Initially, the HCM act more like progenitor cells in that they are not yet fully differentiated. They express the markers
of early stage differentiation such as
GATA-4 and sarcomeric alpha-actin and
have a high capacity for proliferation.
When they are grown to confluency
and cultivated for an extended period of
time, the differentiation process begins.
Markers of late differentiation (e.g. sarcomeric alpha-actinin, slow muscle myosin) are increased and the cells begin to
form myotube-like structures.
Recommended media & reagents
Human Primary
Cells
1.1 Cardiac Myocytes
Fig. 1: HCM culture in phase contrast.

Related products
Human Cardiac Microvascular
Endothelial Cells (see page 20)
Human Cardiac Fibroblasts
(see page 28)
HCM Pellet (see page 91)
PromoFectin Transfection Reagent
(see page 157)
Myocyte Growth Medium (see page 62)

DetachKit
Cryo-SFM
(see page 95)

(see page 79)
Count
Sarcomeric
-actinin negative
Sarcomeric
-actinin positive
Cell Lab Quanta SC TM, Beckman Coulter, Inc.
75
50
Fig. 2: Flow cytometric analysis of PromoCell HCM

after being cultured for 60 days under confluent conditions. 96 % of the cells express sarcomeric -actinin, a
marker of late differentiation.
25
0
0
10
102
103
104
Sarcomeric -actinin
https://www.promocell.com/shop
Product
Human Cardiac Myocytes (HCM)
HCM Pellet
Size
Catalog Number
500,000 cryopreserved cells

500,000 proliferating cells
C-12810
C-12811
1x106 cells in RNAlater
C-14080
12
1.2 Chondrocytes
Applications
Osteoarthritis
research
research
Physiology studies
Chondrocyte differentiation
experiments
Hyaline cartilage investigations
Human Primary
Cells
Joint
produce and maintain the extracellular

matrix of cartilage, i.e. collagen (mostly
type II) and proteoglycans (primarily aggrecan).

Growth Medium
(see page 62)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Chondrocyte
Related products
Pellet (see page 91)
(see page 157)
HCH
Features
Available
from hip and knee joints

tested by Alcian Blue
staining of HCH spheroids
Cryopreserved at second passage
Fig. 1: HCH culture in phase contrast.
Human Chondrocytes (HCH) are isolated from normal human articular cartilage
from the knee and hip joints (lot specific
source information is available on request). PromoCell HCH are routinely
tested for their capacity to differentiate
in 3D spheroids (see Fig. 2).
The articular cartilage covers the joints
between bones and contains no blood
vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which
Fig. 2: Spheroids of differentiated HCH

cultured in Chondrocyte Growth Medium
and stained with Alcian Blue.
Differentiation
Product
Human Chondrocytes (HCH)
HCH Pellet
Size
Catalog Number

C-12710
C-12750
C-14070
13
1.3 Endothelial Cells (Large Vessels)

Applications
Angiogenesis
research
research
Atherosclerosis
Oncology
Drug
uptake studies
junction investigation
Inflammation studies
Cell-cell
Features
Von
Willebrand factor (vWF) positive

positive
Smooth muscle -actin negative
Tunica media
(Smooth muscle cells)
CD31
The endothelium is the thin layer of endothelial cells that line the interior surface
of blood and lymphatic vessels - from the
aorta to the smallest capillaries. In large
vessels (arteries and veins), it is enclosed
by two other layers namely the tunica media and the tunica externa (see Fig. 1).
PromoCell endothelial cells (large vessels) are available from the umbilical
vein and artery, the aorta, the coronary
artery, the pulmonary artery, and the
saphenous vein. All endothelial cells are
tested for positive expression of vWF and
CD31, and the absence of smooth muscle
-actin.
Available endothelial cell types (large vessels):
HUVEC (see below)
HUAEC (see page 14)
HAoEC (see page 15)
HCAEC (see page 15)
HPAEC (see page 16)
HSaVEC (see page 16)
Human Umbilical Vein Endothelial

Cells (HUVEC)
HUVEC are isolated from the vein of the
umbilical cord and are commonly used
for physiological and pharmacological
investigations, such as macromolecule
transport, blood coagulation, angiogenesis, and fibrinolysis.
PromoCell offers HUVEC isolated
in standard Endothelial Cell Growth
Medium and also in Endothelial Cell
Tunica intima
(Endothelial cells)
Fig. 1: Cross section of an artery with the predominant cell types.
Fig. 2: HUVEC culture stained for vWF

(Factor VIII antigen). Nuclei counterstained with DAPI.
Growth Medium 2. The cells are supplied either from single donors* or from
pooled donors (from up to four different
umbilical cords).
In addition, HUVEC are available which
are pre-screened for VEGF (vascular
endothelial growth factor) response.
VEGF is an important signaling protein
involved in both vasculogenesis and
angiogenesis. In vitro, VEGF has been
shown to stimulate endothelial cell
mitogenesis, cell migration, sprouting, and microvascular permeability.
*Human Umbilical Artery Endothelial Cells (HUAEC) from the same donor are available on request.
Fig. 3: HUVEC culture stained for CD31

antigen. Nuclei counterstained with
DAPI.

Cell Growth Medium
(see page 63)
Endothelial Cell Growth Medium 2
(see page 63)
Endothelial
Human Primary
Cells
Tunica externa
(Connective tissue)
14
Human Primary
Cells
Related products
HUVEC single donor Pellet
(see page 92)
HUVEC pooled Pellet (see page 92)
HUVEC Growth Medium 2 single
donor Pellet (see page 92)
HUVEC Growth Medium 2 pooled
HUVEC pre-screened Pellet
(see page 92)
Human
Umbilical Artery Endothelial

Cells (see below)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
VEGF (see page 179)
CD31 and VEGF Antibodies
(see page 169)
Fig. 4: HUVEC culture in phase contrast.
Product
Size
Catalog Number

C-12200
C-12250
C-14010

C-12203
C-12253
C-14011
Human Umbilical Vein Endothelial Cells

(HUVEC) isolated in Growth Medium 2,
single donor

C-12206
C-12207
HUVEC Growth Medium 2 single donor

Pellet
C-14008

pooled

C-12208
C-12209
HUVEC Growth Medium 2 pooled Pellet
C-14009

C-12205
C-12255
C-14012

(HUVEC) single donor
(HUVEC) pooled
HUVEC pooled Pellet

(HUVEC) pre-screened
Human Umbilical Artery

Endothelial Cells (HUAEC)
HUAEC are isolated from the arteries of
the umbilical cord and are often used for
in vitro studies on atherosclerosis but
also for endothelial cell-dependent lymphocyte activation and chemoattractant
activity experiments*.

Endothelial Cell Growth Medium
(see page 63)
(see page 63)
Related products
Human Umbilical Vein Endothelial
Cells (see page 13)
Human Umbilical Artery Smooth
Muscle Cells (see page 39)
CD31 Antibody
(see page 169)
HUAEC
Product
Human Umbilical Artery Endothelial Cells
(HUAEC)
HUAEC Pellet
Size
Catalog Number

C-12202
C-12252
C-14013
*Human Umbilical Vein Endothelial Cells (HUVEC) from the same donor are available on request.
HAoEC are isolated from the human

ascending (thoracic) and descending (abdominal) aorta. They are useful
for studying vascular diseases such as
thrombosis, atherosclerosis, and hypertension as well as for stent-graft compatibility testing.

Cell Growth Medium MV
(see page 64)
Endothelial Cell Growth Medium MV2
(see page 64)
Endothelial
Related products
HAoEC Pellet (see page 91)
Human Aortic Smooth Muscle Cells
(see page 37)
Human Aortic Adventitial Fibroblasts
(see page 28)
CD31 Antibody
(see page 169)
Product
Human Aortic Endothelial Cells (HAoEC)
HAoEC Pellet
Human Coronary Artery

Endothelial Cells (HCAEC)
HCAEC are isolated from the coronary
arteries (the right and the left coronary
artery, including the anterior descending and the circumflex branches) from a
Size
Catalog Number

C-12271
C-12272
C-14023
single donor. They are crucially involved

in the regulation of coronary blood flow
and cardiac functions and are consequently useful for in vitro studies of
diseases such as thrombosis, atherosclerosis, and hypertension. HCAEC are also
suitable for studying functional vaso-
dilators, such as prostacyclin, bradykinin,

or nitric oxide.
(see page 64)
(see page 64)
Endothelial
Related products
Human Coronary Artery Smooth
CD31 Antibody
(see page 169)
HCAEC
Left main coronary

artery
Circumflex coronary
artery
Right coronary artery

Left anterior descending
coronary artery
Fig. 5: Location of the coronary arteries.
Product
Human Coronary Artery Endothelial Cells
(HCAEC)
HCAEC Pellet
Size
Catalog Number

C-12221
C-12222
C-14022
Human Primary
Cells
Human Aortic Endothelial Cells

(HAoEC)
15
16
Human Primary
Cells
Human Pulmonary Artery

Endothelial Cells (HPAEC)
HPAEC are isolated from human pulmonary arteries (the main pulmonary
artery, left and right branches). Since the
pulmonary arteries carry blood from the
heart to the lungs, they are the only arteries (other than umbilical arteries) that
carry deoxygenated blood.

Cell Growth Medium
(see page 63)
(see page 63)
Endothelial
Related products
Human Pulmonary Artery Smooth
CD31 Antibody
(see page 169)
HPAEC
Product
Human Pulmonary Artery Endothelial Cells
(HPAEC)
HPAEC Pellet
Human Saphenous Vein

Endothelial Cells (HSaVEC)
HSaVEC are isolated from human saphenous veins from single donors. The
saphenous vein is often used for autotransplantation in coronary artery bypass
operations, when arterial grafts are not
available or many grafts are required.
Size
Catalog Number

C-12241
C-12242
C-14024

Cell Growth Medium
(see page 63)
(see page 63)
Endothelial
Related products
CD31 Antibody
(see page 169)
HSaVEC
Product
Human Saphenous Vein Endothelial Cells
(HSaVEC)
HSaVEC Pellet
Size
Catalog Number

C-12231
C-12232
C-14025
17
1.4 Endothelial Cells (Microvascular)

Applications
Angiogenesis
research
Oncology
Drug
uptake studies
junction investigation
Inflammation studies
at the interface between the circulating

fluid in the lumen and the surrounding
tissue, microvascular endothelial cells are
highly active and closely involved in numerous physiological processes.
Features
Von
Willebrand factor (vWF) positive

positive
Smooth muscle -actin negative
CD31
The walls of capillaries are composed of a

single layer of microvascular endothelial
cells. This layer is so thin that molecules
such as oxygen, water, and lipids can pass
through it by diffusion and enter the surrounding tissues. Waste products such
as carbon dioxide and urea, on the other
hand, diffuse back into the blood to be
carried away. Because of their location
Human Dermal Microvascular

Endothelial Cells (HDMEC)
HDMEC are isolated from the dermis of
juvenile foreskin and adult skin (different locations). Since the dermis contains
blood and lymphatic capillaries, HDMEC
comprise Blood and Lymphatic Microvascular Endothelial Cells. Both have a
common origin and can be identified by
several markers.
In addition, HDMEC are available which
are pre-screened for VEGF (vascular endothelial growth factor) response. VEGF
is an important signaling protein involved
in both vasculogenesis and angiogenesis.
In vitro, VEGF has been shown to stimu-
Microvascular endothelial cells differ in

morphologies and properties according
to the tissues supplied by the capillaries.
Therefore, PromoCell microvascular endothelial cells are available from dermal,
lung, cardiac, uterine, bladder, and brain
tissues.
Available endothelial cell types (microvascular):
HDMEC (see below)
HDBEC (see page 18)
HDLEC (see page 19)
HBdMEC (see page 20)
HCMEC (see page 20)
HPMEC (see page 21)
HUtMEC (see page 21)
HBMEC (see page 22)
late endothelial cell mitogenesis, cell

migration, sprouting, and microvascular
permeability.
Fig. 1: HDMEC culture stained for vWF

(Factor VIII antigen). Nuclei counterstained with DAPI.
Fig. 2: HDMEC culture stained for CD31

antigen. Nuclei counterstained with DAPI.
Endothelial
Pericytes (see page 51)

Transfection
VEGF (see page 179)
CD31 Antibody
(see page 169)
Related products
HDMEC adult Pellet (see page 91)
HDMEC pre-screened Pellet
(see page 91)
Fig. 3: HDMEC culture in phase contrast.

(see page 64)
(see page 64)
Human
PromoFectin-HUVEC
HDMEC
Product
Human Dermal Microvascular Endothelial
Cells (HDMEC) juvenile foreskin
HDMEC Pellet
Cells (HDMEC) adult donor
HDMEC adult Pellet
Cells (HDMEC) pre-screened
Size
Catalog Number

C-12210
C-12260
C-14015

C-12212
C-12262
C-14016

C-12215
C-12265
C-14017
Human Primary
Cells
Cell-cell
18
HDBEC are isolated from the dermis of

juvenile foreskin and adult skin (different
locations) from a single donor*.
The cells are routinely analyzed by flow
cytometric analysis and immunofluorescent staining: they are > 95% CD31
positive (see Fig. 4) and stain positive for
von Willebrand factor. In addition, they
are > 90% negative for podoplanin
(see Fig. 5) and stain negative for
smooth muscle specific -actin.
Blood Endothelial Cells have a key function in physiological processes like vessel
tonus, capillary permeability, blood coagulation, fibrolysis, and angiogenesis.
They are also useful for disease studies
of atherosclerosis, tumorigenesis, and
thrombosis.
(see page 64)
Related products
HDBEC adult Pellet (see page 91)
Human Dermal Lymphatic Endothelial
Cells (see page 19)
Podoplanin, CD31, and Prox-1
Antibodies (see page 169)
HDBEC
Endothelial
Count
75
CD31 negative
CD31 positive
50
25
Fig. 4: Flow cytometric analysis of PromoCell HDBEC.

98% of the cells are CD31 positive .
0
0
10
102
103
104
CD31
Count
75
Podoplanin negative
Podoplanin positive
Human Primary
Cells
Human Dermal Blood Endothelial

Cells (HDBEC)
50
25
Fig. 5: Flow cytometric analysis of PromoCell HDBEC.

91% of the cells are podoplanin negative.
0
0
10
102
103
104
Podoplanin
Product
Human Dermal Blood Endothelial Cells
(HDBEC) juvenile foreskin
HDBEC Pellet
Human Dermal Blood Endothelial Cells
(HDBEC) adult donor
HDBEC adult Pellet
Size
Catalog Number

C-12211
C-12214
C-14018

C-12225
C-12226
C-14019
*Human Dermal Lymphatic Microvascular Endothelial Cells (HDLEC) from the same donor are available on request.
HDLEC are isolated from the dermis of

juvenile foreskin and adult skin (different
locations) from a single donor*. The cells
are routinely analyzed by flow cytometric
analysis and immunofluorescent staining:
> 95% of the cells are CD31 positive and
podoplanin positive (see Fig. 6 and 7).
In addition, the cells stain positive for
Prox-1.
Lymphatic vessels transport excess fluids
from tissues to the circulatory system and
are a major component of the immune
system. The transported fluid is nearly

cell free. The vessels are highly permeable, because they lack a continuous
basal membrane. Lymphatic endothelial
cells are involved in pathological alterations of the lymphatic system. During
tumor lymphangiogenesis, the lymphatic
endothelial cells build new vessels that
infiltrate tumors, attract tumor cells, and
induce tumor cell metastasis.
Cell Growth Medium MV2
(see page 64)
Endothelial
DetachKit
Cryo-SFM
(see page 95)

(see page 79)
Related products
HDLEC adult Pellet (see page 91)
Cells (see page 18)
VEGF (see page 179)
Podoplanin, CD31, and Prox-1
Antibodies (see page 169)
HDLEC
Count
75
CD31 positive
CD31 negative
50
25
Fig. 6: Flow cytometric analysis of PromoCell HDLEC.

99% of the cells are CD31 positive.
0
0
10
102
103
104
CD31
Count
75
Podoplanin positive
Cell Lab Quanta SC T M, Beckman Coulter, Inc.
Podoplanin negative
50
25
Fig. 7: Flow cytometric analysis of PromoCell HDLEC.

96% of the cells are podoplanin positive.
0
0
10
102
103
104
Podoplanin
Product
Cells (HDLEC) juvenile foreskin
HDLEC Pellet
Cells (HDLEC) adult donor
HDLEC adult Pellet
Size
Catalog Number

C-12216
C-12218
C-14020

C-12217
C-12219
C-14021
*Human Dermal Blood Endothelial Cells (HDBEC) from the same donor are available on request.
Human Primary
Cells
Human Dermal Lymphatic

Endothelial Cells (HDLEC)
19
20
Human Primary
Cells
Human Bladder Microvascular

Endothelial Cells (HBdMEC)
HBdMEC are isolated from the human
bladder microvasculature. The cells are
routinely analyzed by immunofluorescent staining: they stain positive for
CD31 and von Willebrand factor and
negative for smooth muscle -actin.
HBdMEC are useful in studying the
role of microvascular endothelial
cells in the control of inflammation,
capillary exchange, tissue growth, and
angiogenesis.

(see page 64)
(see page 64)
Endothelial
Related products
CD31 Antibody
(see page 169)
HBdMEC
Product
Human Bladder Microvascular Endothelial
Cells (HBdMEC)
HBdMEC Pellet

Endothelial Cells (HCMEC)
HCMEC are isolated from heart ventricles from single donors. The cells are
routinely analyzed by immunofluorescent staining: they stain positive for
HCMEC intensively interact with cardiomyocytes and therefore have a characteristic phenotype different from other
microvascular endothelial cells. HCMEC
Size
Catalog Number

C-12291
C-12292
C-14026
play an important role in the physiological regulation of coronary blood flow

and capillary exchange. Medical conditions such as hypertrophy, ischemia,
hypertension, and diabetes mellitus are
associated with endothelial dysfunction.
(see page 64)
(see page 64)
Endothelial
Related products
HCMEC Pellet (see page 91)
Human Cardiac Myocytes (see page 11)
Human Cardiac Fibroblasts
(see page 28)
CD31 Antibody
(see page 169)
Product
HCMEC Pellet
Size
Catalog Number

C-12285
C-12286
C-14029
HPMEC are isolated from the lung from

a single donor. The cells are routinely
analyzed by immunofluorescent staining: they stain positive for CD31 and
von Willebrand factor and negative for
smooth muscle -actin.
The lung has a vast endothelial surface
area, which is essential for the exchange
of gases. HPMEC are most appropriate
for studying human lung diseases.

(see page 64)
(see page 64)
Endothelial
Related products
Human Pulmonary Fibroblasts
(see page 28)
Human Small Airway Epithelial Cells
(see page 24)
CD31 Antibody
(see page 169)
HPMEC
Product
Human Pulmonary Microvascular
Endothelial Cells (HPMEC)
HPMEC Pellet
Human Uterine Microvascular

Endothelial Cells (HUtMEC)
HUtMEC are isolated from the myometrium (middle layer of the uterine wall)
from a single donor, who has not been
pre-treated with hormones. The cells
are routinely analyzed by immunofluorescent staining: they stain positive for
During pregnancy HUtMEC propagate
intensely and change their phenotype
and expression pattern in response to
sex steroids. This includes up-regulation
Size
Catalog Number

C-12281
C-12282
C-14027
of HUtMEC nitric oxide synthase activity, modulation of adhesion molecule

expression, and an increase in endothelial cell survival and angiogenic activity.
HUtMEC are involved in medical conditions such as uterine fibroma or adenomyosis.
(see page 64)
(see page 64)
Endothelial
Related products
Human Uterine Fibroblasts
(see page 29)
Human Uterine Smooth Muscle Cells
(see page 41)
CD31 Antibody
(see page 169)
HUtMEC
Product
HUtMEC Pellet
Size
Catalog Number

C-12295
C-12296
C-14028
Human Primary
Cells

21
22
Human Primary
Cells
Human Brain Microvascular

Endothelial Cells (HBMEC)
Human Brain Microvascular Endothelial
Cells (HBMEC) are isolated from cortical
tissue (see Fig. 8) from a single donor.
They are routinely analyzed by flow
cytometry and immunofluorescent staining. The cells are positive for CD31 and
von Willebrand factor and negative for
smooth muscle -actin and CD90.
Brain microvascular endothelial cells are

highly specialized cells responsible for
the formation and functional properties
of the Blood-Brain Barrier (BBB). The
BBB is characterized by cellular tight
junctions and active transport systems
which allow the selective transport of
substances into the brain, and prevent
the entrance of unwanted substances.
Therefore, PromoCell HBMEC are well
suited for studying brain endothelial cell
functions like active transport systems

and drug uptake. Furthermore, neuroinflammation studies as well as tight
junction research can be performed
with HBMEC.
Cell Growth Medium MV2
(see page 64)
Endothelial
Cerebral cortex
Thalamus
Corpus callosum
Hypothalamus
Pituitary gland
Cerebellum
Spinal cord
Fig. 8: Schematic structure of the brain.
Fig. 9: HBMEC in phase contrast.
Product
Cells (HBMEC)
HBMEC Pellet
Size
Catalog Number

Limited Availability
C-12287
C-12288
C-14032
23
1.5 Epithelial Cells
Drug uptake studies

Toxicological research
Wound healing research
Oncology
Cell differentiation studies
Respiratory disease research
Typically, epithelial tissue is made of

tightly packed cells with little intercellular space and is arranged in flat sheets
forming effective barriers between the
underlying tissues and the external environment.
Cytokeratin positive
Available from different tissues
PromoCell offers epithelial cells from different locations:

Airways: Nasal mucosa, trachea, bronchi,
and distal respiratory tract
Breast: Mammary gland
Kidney: Whole kidney and renal cortex
Placenta: Amniotic membrane
The epithelium is the interface between the

body and the external environment. It covers all outside surfaces and inside lumina
e.g. the inner lining of the respiratory
tract. In addition, epithelial cells also make
up exocrine and endocrine glands. The
functions of epithelial cells are diverse and
include absorption, secretion, protection,
transcellular transport, and sensation.
Available Epithelial Cells:

HNEpC (see below)
HTEpC (see below)
HBEpC (see page 24)
HSAEpC (see page 24)
HMEpC (see page 25)
HREpC (see page 25)
HRCEpC (see page 26)
HPlEpC (see page 26)
Human Nasal Epithelial Cells

(HNEpC)
particles that are subsequently transported to the pharynx by cilia on the

epithelial cells. HNEpC are useful for in
vitro studies of these processes.
Features
HNEpC are isolated from normal human

nasal mucosa and stain positive for cytokeratin.
The epithelium in the nasal cavity cleans,
humidifies, and warms inhaled air. In addition, it produces mucus, which binds
Fig. 1: HTEpC culture in phase contrast.

Note the cuboidal shape typical for airway epithelial cells.
Fig. 2: HTEpC culture stained for cytokeratin. Nuclei counterstained with

DAPI.
DetachKit
Cryo-SFM
(see page 95)

(see page 79)
Related products
(see page 157)
HNEpC

Epithelial Cell Growth Medium
(see page 65)
Airway
Product
Human Nasal Epithelial Cells (HNEpC)
HNEpC Pellet
Human Tracheal Epithelial Cells

(HTEpC)
HTEpC are isolated from the surface
epithelium of human trachea and stain
positive for cytokeratin.
The respiratory epithelia are responsible for the lubrication of the lungs,
the maintenance of humidity, and the
cleaning of the respiratory tract. They
Size
Catalog Number

C-12620
C-12621
C-14062
are an important target for drugs, toxins, and carcinogens. In addition, they
are involved in many diseases such as
cystic fibrosis. Consequently, HTEpC are
useful for investigating the function and
pathology of the respiratory system.
(see page 65)
Airway
DetachKit
Cryo-SFM
(see page 95)

(see page 79)
Related products
Human Tracheal Smooth Muscle Cells
(see page 39)
(see page 157)
HTEpC
Human Primary
Cells
Applications
24
Product
Human Tracheal Epithelial Cells (HTEpC)
Human Primary
Cells
HTEpC Pellet
Human Bronchial Epithelial Cells

(HBEpC)
HBEpC are isolated from the surface
epithelium of human bronchi and stain
positive for cytokeratin.
The respiratory epithelia are responsible for the lubrication of the lungs,
the maintenance of humidity, and the
cleaning of the respiratory tract. They
Size
Catalog Number

C-12644
C-12645
C-14064
are an important target for drugs, toxins, and carcinogens. In addition, they
are involved in many diseases such as
cystic fibrosis. Consequently, HBEpC are
useful for investigating the function and
pathology of the respiratory system.
(see page 65)
Airway
DetachKit
Cryo-SFM
(see page 95)

(see page 79)
Related products
Human Bronchial Smooth Muscle
Cells (see page 40)
(see page 157)
HBEpC
Product
Human Bronchial Epithelial Cells (HBEpC)
HBEpC Pellet
Human Small Airway Epithelial

Cells (HSAEpC)
HSAEpC are isolated from the distal portion of the human respiratory tract in the
1 mm bronchiole area. They stain positive for cytokeratin.
The distal respiratory tract mainly consists of pulmonary alveoli, which are
spherical outcroppings of the respiratory
bronchioles and are the primary sites of
gas exchange with the blood.
HSAEpC are qualified for functional
Size
Catalog Number

C-12640
C-12641
C-14063
studies to investigate disorders such as

asthma or pulmonary inflammation.

Airway Epithelial Cell
Growth Medium (see page 66)
Small
Related products
(see page 28)
(see page 157)
HSAEpC
Fig. 3: HSAEpC culture in phase contrast.
Product
(HSAEpC)
HSAEpC Pellet
Size
Catalog Number

C-12642
C-12643
C-14065
HMEpC are isolated from the human

adult mammary gland and stain positive
for cytokeratin.
Hormones extensively control the development of the mammary glands.
Throughout puberty, the glands begin to
develop in females in response to ovarian
hormones. Further on during pregnancy,
rising levels of estrogen and progesterone cause additional growth and differentiation. Finally, due to the presence of
the hormone prolactin in late pregnancy,
milk secretion (lactation) begins.

Since mammary gland cells can easily be
induced to grow and multiply by hormones, HMEpC are very well suited for
developmental investigations. In addition, they are useful for breast cancer in
vitro research since breast cancer usually
originates in the lobules or ducts of the
mammary glands.
Epithelial Cell Growth
Medium (see page 67)
Related products
(see page 157)
HMEpC
Human Primary
Cells
Human Mammary Epithelial Cells

(HMEpC)
25
Mammary
Fig. 4: HMEpC culture in phase contrast.
Product
Human Mammary Epithelial Cells
(HMEpC)
HMEpC Pellet
Human Renal Epithelial Cells

(HREpC)
HREpC are isolated from the adult kidney and stain positive for cytokeratin.
Kidneys have a very complex structure
and contain diverse epithelia such as
those in the convoluted tubules, the
cortical collecting ducts, the calyxes,
Renal cortex
Size
Catalog Number

C-12650
C-12651
C-14066
and the renal pelvis. The epithelial cells

present in the distinct ducts and tubules
differ in their morphology and physiological function. Consequently, HREpC
are comprised of a heterogeneous population of epithelial cells.
Important physiological processes like
osmoregulation and excretion can be
studied with HREpC in vitro.

Medium 2 (see page 68)
Renal
Related products
Human Renal Cortical Epithelial Cells
(see page 26)
(see page 157)
HREpC
Renale capsule
Renal medulla
Renal calices
Renal pelvis
Ureter
Fig. 5: Schematic structure of a kidney.
Fig. 6: HREpC culture in phase contrast.
26
Product
Human Renal Epithelial Cells (HREpC)
Human Primary
Cells
HREpC Pellet
Human Renal Cortical Epithelial

Cells (HRCEpC)
HRCEpC are isolated from the cortex of
the human kidney and stain positive for
cytokeratin. The renal cortex is the outer
portion of the kidney between the renal
capsule and the renal medulla. It contains the renal corpuscles, the proximal
and distal convoluted tubules, and the
Size
Catalog Number

C-12665
C-12666
C-14067
cortical collecting ducts.

Important physiological processes like
osmoregulation and excretion can be
studied with HRCEpC in vitro.
Renal Epithelial Cell Growth
Related products
Human Renal Epithelial Cells
(see page 25)
(see page 157)
HRCEpC
Product
(HRCEpC)
HRCEpC Pellet
Human Placental Epithelial Cells

(HPlEpC)
HPlEpC are isolated from the amniotic
membrane and stain positive for cytokeratin.
Placental Epithelial Cells are of fetal origin and form a single layer at the inner
surface of the amnion. They protect the
fetus against maternal leukocytes, neo-
Size
Catalog Number

C-12660
C-12662
C-14068
plastic cells, and pathogens. Cultured

HPlEpC are suitable for physiological and
pathological studies of amniotic functions.
Related products
Villous Mesenchymal
Fibroblasts (see page 29)
HPlEpC Pellet (see page 92)
(see page 157)
Human
Placental
Product
Human Placental Epithelial Cells (HPlEpC)
HPIEpC Pellet
Size
Catalog Number

C-12395
C-12397
C-14069
27
1.6 Fibroblasts
Wound
healing research
engineering research
Regeneration studies
Toxicological research
Oncology
Epithelial-mesenchymal interaction
studies
Tissue
Features
CD90
positive
Fibroblasts are morphologically heterogeneous with diverse appearances

depending on their location in the body
and their activity. Injury of tissue displays
a proliferative stimulus for fibroblasts and
induces them to produce wound healing
proteins. Therefore, fibroblasts are well
suited for wound healing studies.
Fig. 1: NHDF culture in phase contrast.
Cryopreserved
Available
at second passage
from different tissues
like collagens, glycosaminoglycans,

and glycoproteins. The composition of
the extracellular matrix determines the
physical properties of connective tissues.
Human Primary
Cells
Applications
Fibroblasts are the most common cells

in connective tissue. Their main function
is to maintain the structural integrity of
the connective tissue by continuously
secreting extracellular matrix proteins
Normal Human Dermal Fibroblasts

(NHDF)
NHDF are isolated from the dermis of
juvenile foreskin or adult skin from different locations like the face, the breasts,
the abdomen, and the thighs*. They can
be used for wound healing studies and
dermatological research to investigate
diseases like scleroderma, fibrosarcoma,
fibrosis, xeroderma pigmentosum, and
histiocytoma. Moreover, fibroblasts are
important for cancer research, tissue
regeneration, and tissue engineering
studies.
Available Fibroblasts:
NHDF (see below)
HPF (see page 28)
HAoAF (see page 28)
HCF (see page 28)
HUF (see page 29)
HVMF (see page 29)
Fig. 2: NHDF culture stained for CD90.

Nuclei counterstained with DAPI.
Recommended media & reagents for

NHDF juvenile foreskin
Fibroblast Growth Medium
(see page 70)
Recommended media & reagents for
NHDF adult donor
Fibroblast Growth Medium 2
(see page 70)
Related products
NHDF adult Pellet (see page 92)
Normal Human Epidermal
Keratinocytes (see page 32)
Melanocytes (see page 33)
(see page 157)
FGF-1 and FGF-2 (see page 179)
NHDF
Product
Normal Human Dermal Fibroblasts (NHDF)
juvenile foreskin
NHDF Pellet
Normal Human Dermal Fibroblasts (NHDF)
adult donor
NHDF adult Pellet
Size
Catalog Number

C-12300
C-12350
C-14030

C-12302
C-12352
C-14031
* Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are
available on request.
Human Primary
Cells
28

(HPF)
pulmonary hypertension can also be investigated with cultured HPF.
HPF are isolated from human lung tissue. They are often used for studying
lung tissue repair, tissue remodeling after
injury, or the response after pulmonary
inflammation. Fibroblast disorders like
pulmonary fibrosis or hypoxia-induced

(see page 70)
Related products
(see page 24)
(see page 157)
HPF
Product
Human Pulmonary Fibroblasts (HPF)
HPF Pellet
Human Aortic Adventitial

Fibroblasts (HAoAF)
HAoAF are isolated from human aortic
adventitial tissue. HAoAF can be used
for studies concerning vascular diseases
e.g. neointimal formation, vascular remodeling, atherosclerosis, and hypertension. They are also often used for the
examination of fibroblast disorders like
Size
Catalog Number

C-12360
C-12361
C-14035
fibrosis or other diseases linked to either

imperfect or excessive accumulation of
fibroblasts.
(see page 70)
Related products
(see page 15)
(see page 37)
(see page 157)
HAoAF
Product
(HAoAF)
HAoAF Pellet
Human Cardiac Fibroblasts (HCF)

HCF are isolated from the ventricles of
an adult heart. They play a central role in
the maintenance of the extracellular matrix in the normal heart and the synthesis
of growth factors and cytokines. Under
pathophysiological conditions, cardiac
fibroblasts are involved in scar formation
Size
Catalog Number

C-12380
C-12381
C-14037
after myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. HCF can be
used to study such processes in vitro.
(see page 71)
Related products
Human Cardiac Myocytes (see page 11)
(see page 157)
HCF
Product
HCF Pellet
Size
Catalog Number

C-12375
C-12377
C-14036
HUF are isolated from the uterine wall.

During pregnancy, the uterine wall undergoes deep but reversible structural
and biochemical changes. The uterine
wall is the target of hormones and
growth factors that act simultaneously
and lead to extensive modifications of
the extracellular matrix. This and other
morphological and biochemical effects
can be studied in vitro using HUF.

(see page 70)
Related products
Endothelial Cell (see page 21)
(see page 41)
(see page 157)
HUF
Product
Human Uterine Fibroblasts (HUF)
HUF Pellet
Human Villous Mesenchymal

Fibroblasts (HVMF)
HVMF are isolated from human villous
placental tissue. They can be used as
model systems in organ development
research. In addition, they are useful for
investigating the mechanism regulating
human placental growth and function.
Size
Catalog Number

C-12385
C-12386
C-14038

(see page 70)
Related products
Human Placental Epithelial Cells
(see page 26)
(see page 157)
HVMF
Product
Human Villous Mesenchymal Fibroblasts
(HVMF)
HVMF Pellet
Size
Catalog Number

C-12390
C-12391
C-14034
Human Primary
Cells
29
30
1.7 Follicle Dermal Papilla Cells

Applications
Hair
growth research
testing
Toxicological testing
Drug
Features

Follicle Dermal Papilla Cell Growth
Related products
(see page 157)
HFDPC
Human Primary
Cells
Alkaline
phosphatase positive
at second passage
Available from adult lateral scalp
Available from donors with different
hair colors
Cryopreserved
Human Follicle Dermal Papilla Cells

(HFDPC) are isolated from human dermis originating from the lateral scalp.
Information on donor hair and skin color
is available for each lot. HFDPC stain
positive for alkaline phosphatase.
Fig. 1: HFDPC culture in phase contrast.
Sebaceous gland
Hair shaft
Bulge
Matrix cells
Dermal papillae are embedded in a

laminin and collagen IV rich extracellular
matrix at the base of the hair follicles.
They are essential for the induction and
maintenance of hair growth. Since they
have androgen receptors, they can be
used for in vitro screening of androgen
blocking reagents.
Dermal papilla
Fig. 2: Diagram showing the location of the dermal papilla in the hair follicle.
Product
Human Follicle Dermal Papilla Cells
(HFDPC)
HFDPC Pellet
Size
Catalog Number

C-12071
C-12072
C-14005
31
1.8 Hepatocytes
Drug
testing
Toxicological
testing
studies
Liver physiology research
Liver pathology research
Oncology
Metabolism
Features
Cryopreserved
immediately after
isolation
3 - 6 x 106 viable cells per cryovial
Adherence > 50%
Phase I reaction positive
Phase II reaction positive
Albumin positive
Human Hepatocytes (HHpC) are isolated from the normal livers of single
donors. After isolation, the cells are
immediately cryopreserved and then
intensively quality tested. Test criteria are cell viability, cell adherence
efficiency, phase I metabolism, phase II
metabolism, and albumin synthesis. The
cell adherence efficiency is at least 50%.

The liver is the main metabolic organ
in the body performing glycolysis,
glycogenesis, amino acid metabolism,
lipid metabolism, and ureagenesis. In
addition, it also regulates the blood
sugar level. Furthermore, hepatocytes
eliminate toxic substances soon after
absorption since the nutrition rich blood
from the gastro-intestinal system first
has to pass the liver. This detoxification consists of phase I and phase II
reactions. The most important phase I
enzyme system is the cytochrome P450
system. Consequently, cultured human
hepatocytes are perfectly suited for in
vitro metabolism and toxicity/detoxification studies prior to preclinical or
clinical tests.
Note: For culturing Hepatocytes,
Collagen type I coated culture vessels
are necessary.
Under standard in vitro cell culture
conditions, mature human hepatocytes
usually do not survive for longer than
10-14 days and do not proliferate.

Hepatocyte Growth Medium

(see page 73)
Hepatocyte Maintenance Medium
(see page 73)
Related products
PromoFectin-Hepatocyte Transfection
HHpC
Fig 1. HHpC culture in phase contrast.
Count
Living cells
Dead cells
37,5
25
12,5
Fig. 2: Flow cytometric analysis of PromoCell HHpC

using 7-AAD staining. 77 % viable hepatocytes after
thawing of the cryopreserved cells.
0
0
10
102
103
104
7-AAD
Product
Human Hepatocytes (HHpC)
HHpC Pellet
Size
Catalog Number
3 - 6 x 106 cryopreserved cells
C-12850
C-14085
Human Primary
Cells
Applications
32
1.9 Keratinocytes
Applications
Wound
healing research
development and
differentiation studies
Dermatological research
Drug uptake studies
Pharmaceutical testing
Cosmetic and toxicological testing
Oncology
Human Primary
Cells
Epidermal
Features
body and minimizes moisture loss.

Keratinocytes are also able to produce
a variety of cytokines, growth factors,
interleukins, and complement factors.
Therefore keratinocytes are important
for wound healing, inflammation, and
immune response.
Keratinocyte Growth Medium 2
(see page 74)
positive
from juvenile foreskin or
adult skin from different locations
Available from single and pooled
donors
Related products
NHEK.f single donor Pellet (see page 92)
NHEK.f pooled Pellet (see page 92)
NHEK adult, single donor Pellet

(see page 92)
NHEK adult, pooled Pellet
(see page 92)
(see page 27)
Melanocytes (see page 33)
(see page 157)
Cytokeratin
Available
Normal Human Epidermal Keratinocytes (NHEK) are available from single or

pooled donors isolated from the epidermis
of juvenile foreskin or adult skin from different locations like the face, the breasts,
the abdomen, and the thighs*. They are
the major cell type in the epidermis, making up about 90% of the cells.
Epidermal keratinocytes originate in the
stratum basale and move up through
the layers of the epidermis. During this
movement, they undergo gradual differentiation and morphology changes until
they reach the stratum corneum, where
they form a layer of nucleus-free, flat,
and highly keratinized squamous cells.
This layer forms an effective barrier to
the entry of infectious agents into the
Stratum corneum
Stratum granulosum
Stratum spinosum
Stratum basale
Fig. 1: Structure of the epidermis.
Fig. 2: NHEK culture in phase contrast.

Note the cobblestone shape typical for
keratinocytes.
Fig. 3: NHEK culture stained for Cytokeratin. Nuclei counterstained with

DAPI.
Product
Normal Human Epidermal Keratinocytes
(NHEK) juvenile foreskin, single donor
NHEK.f single donor Pellet
(NHEK) juvenile foreskin, pooled
NHEK.f pooled Pellet
(NHEK) adult, single donor
(NHEK) adult, pooled
NHEK adult, pooled Pellet
Size
Catalog Number

C-12001
C-12002
C-14001

C-12005
C-12007
C-14003

C-12003
C-12004
C-14002

C-12006
C-12008
C-14004
* Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available
on request.
33
1.10 Melanocytes
Dermatological
research
research
Melanogenesis studies
Cosmetic and toxicological testing
Skin compound screening
Melanoma
Features
Available
from juvenile foreskin or

adult skin from different locations
Available from light, moderate, and
dark skin types
Donor hair and eye color available on
request
Mel-5 positive
Cultured in PMA-containing or
PMA-free Medium
Normal Human Epidermal Melanocytes
(NHEM) are isolated from the epidermis
of juvenile foreskin or adult skin from
different locations including the face, the
breasts, the abdomen, and the thighs*.
They are located in the stratum basale,
but branch out between the keratinocytes in suprabasal layers (see Fig. 1).
About 5-10 % of the cells in the epidermis are melanocytes.
The main purpose of melanocytes is
the production of melanin, the protein
responsible for the pigmentation of the
skin, eyes, and hair. Melanin protects the
cells in the skin and in deeper layers from
the hazardous effects of UV radiation. It
is produced and stored in melanosomes,
which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.
PromoCell melanocytes are isolated
using either the serum-free, PMA (Phorbol Myristate Acetate)-containing Melanocyte Growth Medium or the serumfree and PMA-free Melanocyte Growth
Medium M2.
Since PMA is a tumor promoting mitogen, it can interfere with experimental
approaches. Therefore, we recommend using cells isolated in Melanocyte
Growth Medium M2 and also using this
medium for cultivation.
Melanocyte Growth Medium
(see page 75)
Melanocyte
Growth Medium M2
(see page 75)
Related products
NHEM.f M2 Pellet (see page 92)
NHEM M2 adult Pellet (see page 92)
(see page 27)
Keratinocytes (see page 32)
(see page 157)
NHEM.f
Stratum corneum
Stratum granulosum
Stratum spinosum
Stratum basale
Melanosoma
Keratinocytes
Melanocyte
Fig. 1: Structure of the epidermis with

the location of a melanocyte.
Fig. 2: NHEM cultured in Melanocyte

Growth Medium in phase contrast.
Fig. 3: NHEM cultured in Melanocyte

Growth Medium M2 in phase contrast.
Product
(NHEM) juvenile foreskin
NHEM.f Pellet
(NHEM) juvenile foreskin, cultured in M2
Medium
NHEM.f M2 Pellet
(NHEM) adult donor, cultured in M2
Medium
NHEM M2 adult Pellet
Size
Catalog Number

C-12400
C-12450
C-14040

C-12402
C-12452
C-14042

C-12403
C-12453
C-14043
* Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.
Human Primary
Cells
Applications
34
1.11 Osteoblasts
Applications
Bone
differentiation
remodeling
Bone physiology research
Oncology
Inflammatory studies
Osteoporosis research
Human Primary
Cells
Bone
Features
From
knee and hip femoral tissue

at second passage
Alkaline phosphatase positive
Tested for mineralization
Growth and Mineralization Media
available
Cryopreserved
Human Osteoblasts (HOB) are isolated

from femoral trabecular bone tissue from
the knee or hip joint region (lot specific
source information is available on request). They stain positive for alkaline
phosphatase and can be mineralized using PromoCell Mineralization Medium.
Osteoblasts are of mesenchymal origin
and are mainly responsible for bone formation and remodeling. In vivo, they
produce a collagen type I rich extracellular
matrix called the osteoid.
During their development, osteoblasts
undergo a maturation process called
osteogenesis. When they become encased in the bone matrix, they stop proliferating and become osteocytes.

Osteoblast Growth Medium
(see page 76)
Osteoblast Mineralization Medium
(see page 76)
Fig. 2: Alkaline phosphatase activity in

HOB stained with BCIP/NBT.
Fig. 3: Mineralized HOB. Calcium deposits stained with Alizarin Red S.
Related products
(see page 157)
HOB
For more information please see

www.promocell.com/knowledge-base
Fig. 1: HOB culture in phase contrast.
Product
Human Osteoblasts (HOB)
HOB Pellet
Size
Catalog Number

C-12720
C-12760
C-14071
35
1.12 Preadipocytes
Proliferation/differentiation
Physiological
studies
and pathological
research
metabolism research
Obesity/diabetes studies
Oncology (e.g. breast cancer)
Fat
Features
From
subcutaneous and visceral

adipose tissue
Available from different locations
Differentiation capacity to adipocytes
Growth and Differentiation Media
available
Human White Preadipocytes (HWP) are
isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is
available on request).
Adipose tissue is crucial in energy storage

and metabolic homeostasis. An increase
in adipose tissue results either from an
enlargement of mature adipocytes or
from the differentiation of adipocyte
precursor cells (preadipocytes) into new
mature adipocytes. These preadipocytes
are already present in adipose tissues.
They can proliferate throughout adult
life and replace the cells that have differentiated into mature adipocytes.
Since this differentiation process is controlled by a variety of growth factors and
hormones, preadipocytes are suitable
for the investigation of the physiological mechanisms controlling proliferation,
differentiation, and function of adipose
tissue.

Preadipocyte Growth Medium
(see page 77)
Preadipocyte Differentiation Medium
(see page 77)
Adipocyte Nutrition Medium
(see page 77)
Related products
subcutaneous Pellet
(see page 92)
HWP visceral Pellet (see page 92)
(see page 157)
HWP
The PromoCell adipocyte cell culture

system provides preadipocytes with optimized growth media and serum-free
differentiation media.
Fig. 1: Left: Undifferentiated HWP (phase contrast). Middle: HWP after in vitro differentiation into adipocytes using the PromoCell
Preadipocyte Differentiation Medium (phase contrast). Right: HWP after in vitro differentiation into adipocytes (Lipid droplets
stained with Oil Red O).
Product
Human White Preadipocytes (HWP)
subcutaneous
HWP subcutaneous Pellet
visceral
HWP visceral Pellet
Size
Catalog Number

C-12730
C-12731
C-14072

C-12732
C-12733
C-14073
Human Primary
Cells
Applications
36
1.13 Skeletal Muscle Cells

Applications
Human Primary
Cells
Gene therapy research

Transplantation research
Developmental/differentiation
studies
Tissue repair research
Pharmacological testing
Neuromuscular disease research
Features
liferate and fuse with damaged muscle

fibers or with one another forming new
myotubes.
SkMC are optimal for in vitro muscle
studies. They proliferate very well in
the mitogen-rich PromoCell Skeletal
Muscle Cell Growth Medium. Fusion to
myotubes with typical multinucleated
syncytia can be induced by using the
PromoCell Skeletal Muscle Cell Differentiation Medium.

Skeletal Muscle Cell Growth Medium
(see page 78)
Skeletal Muscle Cell Differentiation
Related products
(see page 157)
SkMC
Sarcomeric myosin positive

Available from different muscles
Differentiation capacity to multi nucleate syncytia
Growth and Differentiation Media
available
Human Skeletal Muscle Cells (SkMC) are
isolated from different skeletal muscles
(e.g. Musculus pectoralis major) from
adult single donors (lot specific source
information is available on request).
They are positive for sarcomeric myosin
and negative for smooth muscle specific
-actin.
New skeletal muscle cells originate from
quiescent satellite cells, which are located in the muscle fibers between the basal
lamina and the sarcolemma. Quiescent
satellite cells are activated by stimuli such
as muscle damage. After activation, the
cells, now called myoblasts, start to pro-
Fig. 1: SkMC culture in phase contrast.
Fig. 2: Differentiated SkMC culture in

phase contrast. Arrows indicate multinucleated syncytia.

Product
Human Skeletal Muscle Cells (SkMC)
SkMC Pellet
Size
Catalog Number

C-12530
C-12580
C-14060
37
1.14 Smooth Muscle Cells
Organ
specific drug testing

matrix interaction
Cell-extracellular
studies
Physiological
and biochemical studies
Oncology
Pathological
studies
(e.g. hypertension)
Atherosclerosis research
the walls of blood vessels and hollow organs like the bladder, the uterus, and the
gastrointestinal tract and are responsible
for involuntary movements like peristaltic contractions, which propel food along
the gastrointestinal tract.
PromoCell Smooth Muscle Cells are
available from the aorta, the coronary
artery, the pulmonary artery, the umbilical artery, the trachea, the bronchi, the
bladder, the prostate, and the uterus.
Fig. 1: HTSMC culture in phase contrast.

Human Primary
Cells
Applications
Features
muscle -actin positive
CD90 negative
Routinely morphology tested
Smooth
Smooth muscle cells, which form nonstriated muscles, are structurally and
functionally different from skeletal and
cardiac muscle cells. They are located in
Available Smooth Muscle Cells:

HAoSMC (see below)
HCASMC (see page 38)
HPASMC (see page 38)
HUASMC (see page 39)
HTSMC (see page 39)
HBSMC (see page 40)
HBdSMC (see page 40)
HPrSMC (see page 41)
HUtSMC (see page 41)
Fig. 2: HTSMC culture stained for smooth

muscle -actin. Nuclei counterstained
with DAPI.
Human Aortic Smooth Muscle

Cells (HAoSMC)
HAoSMC are isolated from plaque-free
regions of the human aorta (see Fig. 3)
and stain positive for smooth muscle
-actin. The pulsatile pressure produced
by the heart causes a cyclic distention
of the aorta. The smooth muscle cells in
the aortic wall subsequently contract it
again. Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly
influence this process.
Tunica externa
(Connective tissue)
Tunica media
(Smooth muscle cells)
HAoSMC are suitable for studying the

role of smooth muscle cells under normal
or disease conditions in vitro.
Tunica intima
(Endothelial cells)
Fig. 3: Diagram of a blood vessel.

Smooth Muscle Cell Growth
Related products
HAoSMC Pellet (see page 91)
Cryo-SFM (see page 79) (see page 28)
Human
Aortic Endothelial Cells

(see page 15)
(see page 157)
Product
(HAoSMC)
HAoSMC Pellet
Size
Catalog Number

C-12533
C-12532
C-14053
38

Muscle Cells (HCASMC)
Left main coronary
artery
Human Primary
Cells
HCASMC are isolated from human coronary arteries (see Fig. 4) and stain positive for smooth muscle -actin. Coronary arteries supply the heart with blood
and obstruction in atherosclerosis causes
myocardial infarction.
Circumflex coronary
artery
HCASMC are qualified to study the

function of smooth muscle cells in the
development of atherosclerotic plaques
or in other diseases like hypertension.
They are also useful for stent-graft compatibility testing.
Right coronary
artery
Left anterior descending

coronary artery
Fig. 4: Location of the coronary arteries.

Related products
Human Coronary Artery Endothelial
Cells (see page 15)
HCASMC
PromoFectin
Transfection Reagent
(see page 157)
Product
Human Coronary Artery Smooth Muscle
Cells (HCASMC)
HCASMC Pellet
Size
Catalog Number

C-12511
C-12512
C-14052

Muscle Cells (HPASMC)
DetachKit
HPASMC are isolated from human pulmonary arteries and stain positive for
smooth muscle -actin. They are suited
for studying the function of pulmonary
arteries under normal and disease conditions (e.g. vasoconstriction induced
by alveolar hypoxia). They are also useful for physiological studies of vascular
smooth muscle cells.
Related products
Human Pulmonary Artery Endothelial
Cells (see page 16)
(see page 157)

Cryo-SFM
(see page 95)

(see page 79)
HPASMC
Fig. 5: Top: HPASMC culture in phase

contrast. Bottom: HPASMC culture
stained for smooth muscle -actin.
Nucleus counterstained with DAPI.
Product
Human Pulmonary Artery Smooth Muscle
Cells (HPASMC)
HPASMC Pellet
Size
Catalog Number

C-12521
C-12522
C-14051
HUASMC are isolated from the arteries

of the umbilical cord and stain positive
for smooth muscle -actin. Physiological
and biochemical processes of vascular
diseases can be analyzed with HUASMC
in culture.

Related products
HUASMC Pellet (see page 92)
Human Umbilical Artery Endothelial
Cells (see page 14)
(see page 157)
Product
Human Umbilical Artery Smooth Muscle
Cells (HUASMC)
HUASMC Pellet
Size
Catalog Number

C-12500
C-12550
C-14050
Human Tracheal Smooth Muscle

Cells (HTSMC)
Paries
membranaceus
HTSMC are isolated from the trachea

(see Fig. 6) from single donors and stain
positive for smooth muscle -actin. They
connect the endings of the tracheal
cartilage by a contractile bridge called
the paries membranaceus and consequently regulate the airway lumen. Under pathological conditions like asthma,
the amount of smooth muscle cells in
the trachea walls is increased leading to
smooth muscle hypertrophy or hyperplasia. Hence, HTSMC are suitable for in
vitro studies of asthma and other pulmonary diseases.
Related products
HTSMC
Hyaline cartilage
Mucosa
Fig. 6: Cross section through the trachea with c-shaped cartilage connected by the
tracheal muscles.
Human
Tracheal Epithelial Cells

(see page 23)
(see page 157)
Product
(HTSMC)
HTSMC Pellet
Size
Catalog Number

C-12565
C-12566
C-14056
Human Primary
Cells
Human Umbilical Artery Smooth

Muscle Cells (HUASMC)
39
40
Human Primary
Cells

Cells (HBSMC)
HBSMC are isolated from the bronchi
from single donors and stain positive for
smooth muscle -actin. They connect
the endings of the bronchial cartilage
and consequently regulate the airway lumen. Under pathological conditions like
asthma, the amount of smooth muscle
cells in the bronchi walls is increased
leading to smooth muscle hypertrophy

or hyperplasia. Hence, HBSMC are suitable for in vitro studies of asthma and
other pulmonary diseases.
Related products
(see page 24)
(see page 157)
HBSMC
Product
Human Bronchial Smooth Muscle Cells
(HBSMC)
HBSMC Pellet
Size
Catalog Number

C-12561
C-12562
C-14055
Human Bladder Smooth Muscle

Cells (HBdSMC)
HBdSMC are isolated from the detrusor
muscle from the urinary bladder (see
Fig. 7) of single donors and stain positive for smooth muscle -actin. The thick
bladder wall contains smooth muscle cells
arranged in muscle bundles, which empty
the bladder by coordinated contraction.
HBdSMC can be used for in vitro studies
of physiological processes as well as the
effect of drugs on the bladder.
Mucosa
Submucosa
Detrusor Muscle
Adventitia
Fig. 7: Diagram of the bladder.
Related products
(see page 157)
HBdSMC
Product
Human Bladder Smooth Muscle Cells
(HBdSMC)
HBdSMC Pellet
Size
Catalog Number

C-12571
C-12572
C-14057
41
Human Prostate Smooth Muscle

Cells (HPrSMC)
HPrSMC are isolated from the prostate
from single donors and stain positive
for smooth muscle a-actin. The prostate produces secretions that get into
the urethra through contraction of the
Prostate smooth muscle cells can be involved in prostitis and benign prostatic
hyperplasia (BPH). HPrSMC can be used
to study these prostate diseases or physiological processes in vitro.
Lateral lobes
Capsule
Human Primary
Cells
Verumontanum
Ejaculatory duct
Fig. 8: Diagram of the prostate.

PromoFectin
(see page 157)
Related products
HPrSMC Pellet (see page 92)
Product
Human Prostate Smooth Muscle Cells
(HPrSMC)
Size
Catalog Number

C-12573
C-12574
C-14058
HPrSMC Pellet
Human Uterine Smooth Muscle

Cells (HUtSMC)
HUtSMC are isolated from the myometrium (the middle layer of the uterine wall,
see Fig. 9) and stain positive for smooth
muscle -actin. During pregnancy, hormones and growth factors cause an extensive hypertrophy of uterine smooth
muscle cells.
This and other morphological and biochemical effects on the uterus can be
studied in vitro using HUtSMC.
Uterine tube
Perimetrium (peritoneum)
Myometrium (muscle)
Uterine cavity
Endometrium (epithelium)
Cavity of cervix
Vagina
Fig. 9: Diagram of the uterus.
Human
Uterine Microvascular
Related products
HUtSMC Pellet (see page 92)
Fig. 9: Diagram of the uterus.
Product
(HUtSMC)
HUtSMC Pellet
Size
Catalog Number

C-12575
C-12576
C-14059
42
Overview Human Primary Cells
Population
Doublings
Page
Sarcomeric -actinin+
Slow muscle myosin+
> 15
11
P2
Differentiation tested
> 10
12
5,000 - 10,000
cells per cm
P1
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
13
C-22010
C-22011
5,000 - 10,000
cells per cm
P1
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
13
C-12206
C-22011
5,000 - 10,000
cells per cm
P1
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
13
Human Umbilical Vein

Endothelial Cells (HUVEC)
isolated in Growth Medium 2,
pooled
C-12208
C-22011
5,000 - 10,000
cells per cm
P1
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
13

pre-screened
C-12205
C-22010
C-22011
5,000 - 10,000
cells per cm
P1
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
13

C-12202
C-22010
C-22011
5,000 - 10,000
cells per cm
P1
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
14
Human Aortic
Endothelial Cells (HAoEC)
C-12271
C-22020
C-22022
5,000 - 10,000
cells per cm
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
15

C-12221
C-22020
C-22022
5,000 - 10,000
cells per cm
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
15

C-12241
C-22010
C-22011
5,000 - 10,000
cells per cm
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
16

C-12231
C-22010
C-22011
5,000 - 10,000
cells per cm
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
16

juvenile foreskin
C-12210
C-22020
C-22022
10,000 - 20,000
cells per cm
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
17

adult donor
C-12212
C-22020
C-22022
10,000 - 20,000
cells per cm
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
17

pre-screened
C-12215
C-22020
C-22022
10,000 - 20,000
cells per cm
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
17
Human Dermal Blood

Endothelial Cells (HDBEC)
juvenile foreskin
C-12211
C-22020
10,000 - 20,000
cells per cm
P2
PodoplaninProx-1CD31+
> 15
18
Human Dermal Blood

Endothelial Cells (HDBEC)
adult donor
C-12225
C-22020
10,000 - 20,000
cells per cm
P2
PodoplaninProx-1CD31+
> 15
18

juvenile foreskin
C-12216
C-22022
10,000 - 20,000
cells per cm
P2
Podoplanin+
Prox-1+
CD31+
> 15
19

adult donor
C-12217
C-22022
10,000 - 20,000
cells per cm
P2
Podoplanin+
Prox-1+
CD31+
> 15
19

C-12291
C-22020
C-22022
10,000 - 20,000
cells per cm
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
20
Human Primary
Cells
Product
Cat. No.
Cryopr. Cells
Recommended
Culture Media*
Plating Density
Passage after
Thawing
Human Cardiac Myocytes

(HCM)
C-12810
C-22070
10,000 - 15,000
cells per cm
P2
C-12710
C-27101
10,000 - 20,000
cells per cm

single donor
C-12200
C-22010
C-22011

pooled
C-12203

isolated in Growth Medium 2,
single donor
* The media in this table are ready-to-use. For Media Kits see page 60.
Marker
43
Population
Doublings
Page
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
20
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
21
10,000 - 20,000
cells per cm
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 15
21
C-22022
10,000 - 20,000
cells per cm
P2
vWF+
CD31+
Dil-Ac-LDL uptake+
> 10
22
C-12620
C-21060
10,000 - 15,000
cells per cm
P2
Cytokeratin+
> 15
23
Human Tracheal
Epithelial Cells (HTEpC)
C-12644
C-21060
10,000 - 15,000
cells per cm
P2
Cytokeratin+
> 15
23
Human Bronchial
Epithelial Cells (HBEpC)
C-12640
C-21060
10,000 - 15,000
cells per cm
P2
Cytokeratin+
> 15
24
Human Small Airway

Epithelial Cells (HSAEpC)
C-12642
C-21070
10,000 - 15,000
cells per cm
P2
Cytokeratin+
> 15
24
Human Mammary
Epithelial Cells (HMEpC)
C-12650
C-21010
10,000 - 15,000
cells per cm
P2
Cytokeratin+
> 15
25
Human Renal
Epithelial Cells (HREpC)
C-12665
C-26001
C-26030
10,000 - 15,000
cells per cm
P2
Cytokeratin+
> 15
25
Human Renal Cortical

Epithelial Cells (HRCEpC)
C-12660
C-26001
C-26030
10,000 - 15,000
cells per cm
P2
Cytokeratin+
> 15
26
Human Placental
Epithelial Cells (HPlEpC)
C-12395
C-26040
10,000 - 15,000
cells per cm
P2
Cytokeratin+
>5
26
Normal Human Dermal

Fibroblasts (NHDF)
juvenile foreskin
C-12300
C-23010
3,500 - 7,000
cells per cm
P2
CD90+
> 15
27
Normal Human Dermal

Fibroblasts (NHDF)
adult donor
C-12302
C-23020
3,500 - 7,000
cells per cm
P2
CD90+
> 15
27
Human Pulmonary
Fibroblasts (HPF)
C-12360
C-23020
3,500 - 7,000
cells per cm
P2
CD90+
> 15
28

Fibroblasts (HAoAF)
C-12380
C-23020
3,500 - 7,000
cells per cm
P2
CD90+
> 10
28
Human Cardiac
Fibroblasts (HCF)
C-12375
C-23025
3,500 - 7,000
cells per cm
P2
CD90+
> 15
28
Human Uterine
Fibroblasts (HUF)
C-12385
C-23020
3,500 - 7,000
cells per cm
P2
CD90+
> 15
29

Fibroblasts (HVMF)
C-12390
C-23020
3,500 - 7,000
cells per cm
P2
CD90+
> 15
29
Human Follicle Dermal

Papilla Cells (HFDPC)
C-12071
C-26501
5,000 - 10,000
cells per cm
P2
Alkaline
phosphatase+
> 10
30
C-12850
C-25010
C-25020
150,000
cells per cm
P0
Phase I reaction+
Phase II reaction+
Albumin+
n/a
31

Keratinocytes (NHEK)
juvenile foreskin, single donor
C-12001
C-20011
5,000
cells per cm
P2
Cytokeratin+
> 15
32

juvenile foreskin, pooled
C-12005
C-20011
5,000
cells per cm
P2
Cytokeratin+
> 15
32

adult, single donor
C-12003
C-20011
5,000
cells per cm
P2
Cytokeratin+
> 15
32
Product
Cat. No.
Cryopr. Cells
Recommended
Culture Media*
Plating Density
Passage after
Thawing

C-12285
C-22020
C-22022
10,000 - 20,000
cells per cm
P2
Human Pulmonary
Microvascular
C-12281
C-22020
C-22022
10,000 - 20,000
cells per cm

C-12295
C-22020
C-22022
Human Brain Microvascular

Endothelial Cells (HBMEC)
C-12287
Human Nasal
Epithelial Cells (HNEpC)
Marker
Human Primary
Cells
44
Population
Doublings
Page
Cytokeratin+
> 15
32
P2
Mel-5+
> 15
33
5,000 - 10,000
cells per cm
P2
Mel-5+
> 15
33
C-24300
5,000 - 10,000
cells per cm
P2
Mel-5+
> 15
33
C-12720
C-27001
C-27020
10,000 - 20,000
cells per cm
P2
Alkaline
phosphatase+
Mineralization tested
> 10
34
Human White Preadipocytes

(HWP) subcutaneous
C-12730
C-27410
C-27436
C-27438
5,000
cells per cm
P2
Differentiation
tested
> 10
35
Human White Preadipocytes

(HWP) visceral
C-12732
C-27410
C-27436
C-27438
5,000
cells per cm
P2
Differentiation
tested
> 10
35
Human Skeletal Muscle Cells

(SkMC)
C-12530
C-23060
C-23061
3,500 - 7,000
cells per cm
P2
Sarcomeric myosin+
Differentiation tested
> 15
36
Human Aortic Smooth Muscle

Cells (HAoSMC)
C-12533
C-22062
7,500 - 10,000
cells per cm
P2
Smooth muscle
-actin+
> 15
37

Smooth Muscle Cells (HCASMC)
C-12511
C-22062
7,500 - 10,000
cells per cm
P2
Smooth muscle
-actin+
> 15
38

Smooth Muscle Cells (HPASMC)
C-12521
C-22062
7,500 - 10,000
cells per cm
P2
Smooth muscle
-actin+
> 15
38

Smooth Muscle Cells (HUASMC)
C-12500
C-22062
7,500 - 10,000
cells per cm
P2
Smooth muscle
-actin+
> 15
39
Human Tracheal
Smooth Muscle Cells (HTSMC)
C-12565
C-22062
7,500 - 10,000
cells per cm
P2
Smooth muscle
-actin+
> 15
39
Human Bronchial
Smooth Muscle Cells (HBSMC)
C-12561
C-22062
7,500 - 10,000
cells per cm
P2
Smooth muscle
-actin+
> 15
40
Human Bladder
Smooth Muscle Cells (HBdSMC)
C-12571
C-22062
7,500 - 10,000
cells per cm
P2
Smooth muscle
-actin+
> 15
40
Human Prostate
Smooth Muscle Cells (HPrSMC)
C-12573
C-22062
7,500 - 10,000
cells per cm
P2
Smooth muscle
-actin+
> 15
41
Human Uterine
Smooth Muscle Cells (HUtSMC)
C-12575
C-22062
7,500 - 10,000
cells per cm
P2
Smooth muscle
-actin+
> 15
41
Human Primary
Cells
Product
Cat. No.
Cryopr. Cells
Recommended
Culture Media*

adult, pooled
C-12006
C-20011
5,000
cells per cm
P2

Melanocytes (NHEM)
juvenile foreskin
C-12400
C-24010
5,000 - 10,000
cells per cm

Melanocytes (NHEM) juvenile
foreskin, cultured in M2 Medium
C-12402
C-24300

Melanocytes (NHEM) adult
donor, cultured in M2 Medium
C-12403
Plating Density
Passage after
Thawing
Marker
45
Human Primary Cells by Tissue Type
Page
Human Mesenchymal Stem Cells

from Adipose Tissue (hMSC-AT)
49

subcutaneous
35

visceral
35
n Bladder
20
Human Bladder
Smooth Muscle Cells (HBdSMC)
40
n Blood/Bone Marrow
from Bone Marrow (hMSC-BM)
49
Human Mononuclear Cells from Peripheral

Blood (hMNC-PB) single donor, ultra-pure
56
Human Mononuclear Cells from Peripheral

Blood (hMNC-PB) pooled, ultra-pure
56
Human Mononuclear Cells from Cord

Blood (hMNC-CB) single donor, ultra-pure
57
Human Mononuclear Cells from

Cord Blood (hMNC-CB) pooled, ultra-pure
57
Human CD34 Progenitor Cells from

Cord Blood (hCD34+-CB) single donor
52
Human CD34+ Progenitor Cells from

Cord Blood (hCD34+-CB) pooled
52
Human CD133 Progenitor Cells from

Cord Blood (hCD133+-CB) single donor
53

Cord Blood (hCD133+-CB) pooled
53
Human CD14 Monocytes from Peripheral

Blood (hMoCD14+-PB), single donor
54
Human CD14+ Monocytes from Peripheral

Blood (hMoCD14+-PB), single donor,
pre-screened
54
Human Monocytes from Peripheral Blood

(hMo-PB), single donor
(hMo-PB), single donor, pre-screened
Page
n Brain
n Adipose Tissue
Product

Cells (HBMEC)
22
n Heart
28
Human Cardiac Myocytes (HCM)
11

20
n Kidney
Human Renal Epithelial Cells (HREpC)
25

(HRCEpC)
26
n Large Vessels
Fibroblasts (HAoAF)
28
Human Aortic Endothelial Cells (HAoEC)
15
Human Aortic
Smooth Muscle Cells (HAoSMC)
37

15

Smooth Muscle Cells (HCASMC)
38

16

Smooth Muscle Cells (HPASMC)
38

14

39
54

Endothelial Cells (HUVEC) single donor
13
54

Endothelial Cells (HUVEC) pooled
13

single donor
13

pooled
13
n Bone/Joint/Skeletal System
34
12

Endothelial Cells (HUVEC) pre-screened
13
Human Skeletal Muscle Cells (SkMC)
36

16
Human Primary
Cells
Product
46
Product
Page
Human Primary
Cells
31
n Mammary Tissue
Human Mammary
Epithelial Cells (HMEpC)
Page
n Respiratory System
n Liver
Product
25
n Umbilical Cord
14

39

Endothelial Cells (HUVEC) single donor
13

Endothelial Cells (HUVEC) pooled
13

single donor
13

pooled
13

Endothelial Cells (HUVEC) pre-screened
from Umbilical Cord Matrix (hMSC-UC)
Human Nasal Epithelial Cells (HNEpC)
23
Human Tracheal Epithelial Cells (HTEpC)
23
Human Tracheal
Smooth Muscle Cells (HTSMC)
39
Human Bronchial Epithelial Cells (HBEpC)
24
Human Bronchial
Smooth Muscle Cells (HBSMC)
40
Human Small Airway

Epithelial Cells (HSAEpC)
24
Human Pulmonary Fibroblasts (HPF)
28

21
n Skin
Endothelial Cells (HDMEC) juvenile foreskin
17

Endothelial Cells (HDMEC) adult donor
17
13

Endothelial Cells (HDMEC) pre-screened
17
49
Human Dermal Blood

Endothelial Cells (HDBEC) juvenile foreskin
18
Human Dermal Blood

Endothelial Cells (HDBEC) adult donor
18

Endothelial Cells (HDLEC) juvenile foreskin
19

Endothelial Cells (HDLEC) adult donor
19
Normal Human Dermal

Fibroblasts (NHDF) juvenile foreskin
27
Normal Human Dermal

Fibroblasts (NHDF) adult donor
27
Human Follicle Dermal

Papilla Cells (HFDPC)
30
n Uterus
29

21
Human Uterine
Smooth Muscle Cells (HUtSMC)
41
n Placenta
Human Pericytes from Placenta (hPC-PL)
51

(NHEK) juvenile foreskin, single donor
32
Human Placental Epithelial Cells (HPlEpC)
26

(NHEK) juvenile foreskin, pooled
32

Fibroblasts (HVMF)
29

(NHEK) adult, single donor
32

(NHEK) adult, pooled
32

Melanocytes (NHEM) juvenile foreskin
33
Normal Human Epidermal Melanocytes (NHEM)

juvenile foreskin, cultured in M2 Medium
33
Normal Human Epidermal Melanocytes (NHEM)

adult donor, cultured in M2 Medium
33
n Prostate
Human Prostate
Smooth Muscle Cells (HPrSMC)
41
Human Stem and

Blood Cells
PromoCell offers a collection of adult stem cells, as well as
differentiated blood cells from normal human bone marrow,
umbilical cord tissue, placenta, adipose tissue, peripheral blood,
and cord blood. All cells are obtained from approved donor programs at associated medical centers. High-quality media that
simulate the in vivo environment as well as optimized differentiation media systems are also available for these cell types.

2.1 Mesenchymal Stem Cells . . . . . . . . . . . . . . 49
2.2 Pericytes . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
2.3 CD34+ Progenitor Cells . . . . . . . . . . . . . . . . 52
2.4 CD133+ Progenitor Cells . . . . . . . . . . . . . . . 53
2.5 Monocytes . . . . . . . . . . . . . . . . . . . . . . . . . 54
2.6 Mononuclear Cells . . . . . . . . . . . . . . . . . . . 56
Overview Human Stem and Blood Cells . . . . . . . . 58
2 Human Stem and Blood Cells
49
2.1 Mesenchymal Stem Cells
cell research
medicine research
Tissue engineering studies
Developmental experiments

Stem
Regenerative
Features
adult stem cells
for in vitro differentiation
Routinely analyzed by flow cytometry
Two Growth Media and five optmized
Differentiation Media available
PromoCell hMSC-BM are harvested from

normal human bone marrow. The cells
are tested for their ability to differentiate
in vitro into adipocytes, chondrocytes,
osteoblasts, and neuronal cells.
Multipotent
Tested
Mesenchymal Stem Cells (MSC), also

termed Mesenchymal Stromal Cells, are
multipotent cells that can differentiate
into a variety of cell types and have the
capacity for self renewal. MSC have
been shown to differentiate in vitro or
in vivo into adipocytes, chondrocytes,
osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized PromoCell media are available to
support both the growth of MSC and
their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into
diverse cell types vary between MSC of
different origin.

from Umbilical Cord Matrix
(hMSC-UC)
PromoCell hMSC-UC are harvested from
normal human umbilical cord matrix
(Whartons jelly). The cells are tested for
their ability to differentiate in vitro into
adipocytes, chondrocytes, osteoblasts,
and neuronal cells.

PromoCell hMSC-AT are harvested from
normal human adipose tissue. The cells
are tested for their ability to differentiate
in vitro into adipocytes, chondrocytes,
osteoblasts, and neuronal cells.

Stem Cell
Growth Medium (see page 80)
Mesenchymal Stem Cell
Growth Medium DXF (see page 80)
Mesenchymal Stem Cell Adipogenic
Differentiation Medium (see page 80)
Mesenchymal Stem Cell Chondrogenic
Differentiation Medium w/o Inducers
(see page 80)
Mesenchymal Stem Cell Osteogenic
Mesenchymal Stem Cell Neurogenic
Cell Dissociation Solution ACF
(see page 96)
Fibronectin Solution, human
(see page 99)
Mesenchymal
Related products
MSC-Qualified
Fetal Calf Serum

(see page 88)
hMSC-BM Pellet (see page 92)
hMSC-UC Pellet (see page 92)
hMSC-AT Pellet (see page 92)

Fig. 1: Undifferentiated hMSC-BM cultured in MSC Growth Medium.
Fig. 2: Undifferentiated hMSC-BM cultured in MSC Growth Medium DXF.
Human Stem and

Blood Cells
Applications
Human Stem and

Blood Cells
50
Fig. 3.1
Fig. 3.1: hMSC-BM after in vitro differentiation into adipocytes using PromoCell
Differentiation Medium (lipid droplets
stained with Oil Red O).
Fig. 3.2: hMSC-BM spheroid after in vitro
differentiation into cartilage using PromoCell
Differentiation Medium (Alcian Blue
staining).
Fig. 3.2
Fig. 3.3: hMSC-BM after in vitro differentiation into osteoblasts using PromoCell
Mesenchymal StemCell Osteogenic Differentiation Medium (Alizarin Red S staining).
Fig. 3.4: hMSC-BM after in vitro differentiation into neuronal cells using PromoCell
Mesenchymal Stem Cell Neurogenic Differentiation Medium.
Fig. 3.3
Fig. 3.4
For additional stem cell related products, please visit

www.promokine.info/products/stem-cell-research
Product
hMSC-BM Pellet
from Umbilical Cord Matrix (hMSC-UC)
hMSC-UC Pellet
hMSC-AT Pellet
Size
Catalog Number

C-12974
C-12975
C-14090

C-12971
C-12972
C-14091

C-12977
C-12978
C-14092
51
2.2 Pericytes
Applications
Angiogenesis
Regenerative
research
medicine research
Oncology
blood vessel walls. They are important

for angiogenesis, the structural integrity of the microvasculature, and blood
flow regulation. However, they can also
develop into malignant tumors called
hemangiopericytomas.
Non-hematopoietic
multipotent
mesenchymal-like cells
Important in angiogenesis
Routinely characterized by flow
cytometry
Isolated from human placenta
Pericytes are multipotent mesenchymallike cells found in association with small
To date, pericytes have demonstrated

the ability to differentiate into adipocytes, osteoblasts, fibroblasts, smooth
muscle cells, and phagocytes (macrophages).
Fig. 4: Proliferating human pericytes

(hPC-PL) from the placenta (phase contrast).

Pericyte Growth Medium
(see page 81)
Related products
hPC-PL Pellet (see page 92)
Count
CD146
50
CD146+
25
Fig. 5: Flow cytometric analysis of PromoCell Pericytes.

> 98% of the cells are CD146+.
0
100
101
102
103
104
CD146
Product
Human Pericytes from Placenta (hPC-PL)
hPC-PL Pellet
Size
Catalog Number

C-12980
C-12981
C-14095
Human Stem and

Blood Cells
Features
52
2.3 CD34+ Progenitor Cells

Applications
Stem
cell research
basic research
Hematological and immunological
studies
Vascular and angiogenesis research
Oncology research
Features
Multipotent
hematopoietic/
endothelial progenitors
CD34+ cell purity > 95%
Superior viability
CD34 is a glycosylated transmembrane
protein and represents a well-known

Hematopoietic Progenitor Cell
Expansion Medium DXF
(see page 81)
Hematopoietic Progenitor Medium
(see page 81)
Endothelial Progenitor Medium
(see page 82)
Related products
(see page 179)
SCF (see page 179)
flt-3 ligand (see page 179)
IL-3 (see page 179)
IL-6 CC (see page 179)
TPO (see page 179)
CD34 antibody
(see page 169)
G-CSF
Count
CD34
Human Stem and

Blood Cells
Transplantation
marker for primitive blood- and bone

marrow-derived progenitor cells, especially for hematopoietic and endothelial
stem cells. Although the biological functions of CD34 are largely unknown, recent data suggest that CD34 is involved
in maintenance of the progenitor cells in
a phenotypically undifferentiated state.
PromoCell offers CD34+ Progenitor Cells
from the cord blood of healthy donors.
The CD34+ Progenitor Cells contain
two main cellular subpopulations, hematopoietic and endothelial progenitor
cells. Therefore, CD34+ Progenitor Cells
are suitable for a series of studies, e.g.
directed differentiation into more committed types of blood cells and endothelial lineages.
CD34+
50
25
Fig. 6: Flow cytometric analysis of PromoCell CD34+

Progenitor Cells. > 95% of the cells are CD34+.
0
0
10
102
103
104
CD34
Product
Size
Catalog Number
Human CD34+ Progenitor Cells from Cord

Blood (hCD34+-CB), single donor
C-12921
Human CD34+ Progenitor Cells

from Cord Blood (hCD34+-CB), pooled
C-12924
53
2.4 CD133+ Progenitor Cells
Hematology
research
cell research
Oncology
Stem
Features
progenitors, e.g. of hemato-
poietic lineages
Available from cord blood
CD133+ cell purity > 95%
Superior viability
Expansion and Differentiation Media
available
CD133 (AC133, Prominin-1) is a glycoprotein with sub-cellular localization in

plasma membrane protrusions. In humans, this protein was originally identified as an antigenic marker expressed
on hematopoietic stem cells. In addition,
CD133 is also expressed by neural and
muscle progenitor cells.
Primitive
CD133 cells are isolated from fresh cord

blood. These cells are highly proliferative
primitive progenitors. They can give rise
to mature terminally differentiated cells,
e.g. hematopoietic lineages, by means
of directed differentiation. PromoCell
also provides suitable expansion and differentiation media.
+

Expansion Medium DXF (see page 81)
Cytokine Mix E for HPC Expansion
Medium DXF (see page 81)
(see page 81)
Related products
G-CSF (see page 179)
SCF (see page 179)
flt-3 ligand (see page 179)
TPO (see page 179)
CD133 antibody
(see page 169)
103
CD133+ cells
102
10
Fig. 7: Flow cytometric analysis of cord blood-derived

CD133+ Progenitor Cells, demonstrating their high
purity.
0
0
10
102
CD45
103
CD133
Product
Size
Catalog Number

Cord Blood (hCD133+-CB), single donor
C-12927

Cord Blood (hCD133+-CB), pooled
C-12929
Human Stem and

Blood Cells
Applications
54
2.5 Monocytes
Applications
Dendritic
Cell Research
(Auto-)Immunity
Infection
biology
Features
Isolated
Human Stem and

Blood Cells
from fresh peripheral blood

analyzed by flow cytometry
CD14+-selected as well as untouched
Monocytes available
gradient-based method completely

devoid of using any immunomagnetic
beads. The advantage of these completely native Monocytes is that potential effects of bound magnetic particles
(which can be ingested by this phagocytic cell type) are excluded.
Routinely
Monocytes (Mo) are immature phagocytic cells circulating in the blood. Acting
as antigen-presenting immune cells, they
can phagocytize and degrade microbes
and particulate matter. Monocytes can
differentiate into several different cell
types. The most common applications
for Monocytes are the in vitro differentiation into monocyte-derived dendritic
cells (moDC), macrophages, and osteoclasts as well as their use as phagocytic
immune cells in immunology and infection biology.
CD14 belongs to the family of LPS receptor antigens and is strongly expressed
on the majority of Monocytes. Thus,
binding of immunomagnetic particles
to this antigen is employed for purification of PromoCell CD14+-Monocytes
(hMoCD14+-PB) from Mononuclear
Cells (MNC).
In contrast, the untouched Monocytes (hMo-PB) provided by PromoCell
are enriched from MNC by a density
Human CD14+ Monocytes

(hMoCD14+-PB)
PromoCell Human CD14+ Monocytes
are isolated from fresh peripheral blood.
After isolation of the ultra-pure mononuclear cell fraction by proprietary methods, the CD14+ Monocytes are isolated
by positive selection with > 95% purity.
In addition, hMoCD14+-PB are available
which are pre-screened for their differentiation capacity into dendritic cells.

Mononuclear Cell Medium (see page 84)
DC Generation Medium (see page 82)
Related products
hMoCD14+-PB single donor Pellet
(see page 91)
hMoCD14+-PB single donor
pre-screened Pellet (see page 92)
hMo-PB single donor Pellet
(see page 92)
hMo-PB single donor
pre-screened Pellet (see page 92)
PromoFectin-Macrophage
(see page 158)
G-CSF (see page 179)
GM-CSF (see page 179)
TNF-alpha (see page 179)
M-CSF (see page 179)
sRANKL (see page 179)
CD14 antibody
(see page 169)
Human Monocytes (hMo-PB)

PromoCell Human untouched Monocytes are isolated from fresh peripheral
blood. After isolation of the ultra-pure
mononuclear cell fraction by proprietary
methods, the Monocyte fraction is further purified to > 70% by an advanced
multi-gradient technique.
In addition, hMo-PB are available which
are pre-screened for their differentiation
capacity into dendritic cells.
Fig. 8: Cluster formation during

differentiation of Monocytes into moDC.

Fig. 9: Mature moDC cultured in

PromoCell DC Generation Medium showing typical dendrite-like structures.
55
CD14+
CD14-
12,5
Fig. 10a: Flow cytometric analysis of day 8 mature

moDCs generated in the PromoCell DC Generation
Medium. More than 98% of the cells are negative for
CD14.
0
0
101
102
103
104
103
104
CD14
Count
CD83+
CD83-
12,5
Fig. 10b: Flow cytometric analysis of day 8 mature

moDCs generated in the PromoCell DC Generation
Medium. The DC maturation marker CD83 is strongly
expressed by > 98% of the cells.
0
0
101
102
CD83
Product
Size
Catalog Number
10 x 106 cryopreserved cells
C-12909
C-14110
C-12912
C-14112
C-12910
C-14111

(hMo-PB), single donor, pre-screened
C-12913
hMo-PB single donor pre-screened Pellet
C-14113

Blood (hMoCD14+-PB), single donor
Blood (hMoCD14+-PB), single donor,
pre-screened
pre-screened Pellet
(hMo-PB), single donor
Human Stem and

Blood Cells
Count
56
2.6 Mononuclear Cells

Applications
Stem
cell research
basic research
Hematological and immunological
studies
Oncology research
Virology and infection biology
Features
Routinely
analyzed by flow cytometry

content < 5%
Red blood cells depleted without lysis
High purity and viability
Available from single and pooled
donors
Granulocyte
matopoietic and endothelial progenitors,

monocytes, and lymphocyte subpopulations (see Fig. 12).

Peripheral Blood (hMNC-PB)
Adult peripheral blood contains large
numbers of mature immune cells but
also some progenitor cells. Typical application fields for hMNC-PB from single
donors are immunology, oncology, hematology, virology, and infection biology.
PromoCell hMNC-PB pooled are preferably used for several types of infectious
virus tests, including neutralization assays. For HIV-related research, prior
CD8+ T-cell depletion is recommended.
Size [m]
Granulocytes
Monocytes
16
14
Lymphocytes
12
Fig. 11: Flow cytometric analysis of Mononuclear

Cells from Cord Blood (hMNC-CB). Granulocyte
content 1%.
10
8
0
0
10
102
103
104
Side Scatter
CD45
104
CD34+ cells
103
Human Stem and

Blood Cells
Transplantation
PromoCell Mononuclear Cells (MNC)

represent the lymphocyte and monocyte
fraction isolated from umbilical cord blood
or peripheral blood. They are prepared
by optimized low-density gradient centrifugation, which effectively reduces the
granulocyte content (see Fig. 11). Red
blood cells are gently but thoroughly depleted by proprietary techniques instead
of osmotic lysis. This results in ultra-pure
mononuclear cells with superior viability
and unchanged biological function. In
addition, the cells do not clump after
thawing, allowing for easy handling and
reproducible results.
The MNC can be used directly for experiments or as a starting material for the
isolation of several cell types, e.g., he-
102
10
Fig. 12: Flow cytometric determination of CD34+ cells

within the PromoCell Mononuclear Cells from Cord
Blood (hMNC-CB).
0
0
10
102
CD34
103
104
hMNC-CB are isolated from umbilical

cord blood, which has a much higher
content of primitive progenitor cells compared to adult peripheral blood.
Mononuclear Cell Medium
(see page 84)
(see page 81)
Endothelial
Progenitor Medium
(see page 82)
Expansion Medium DXF (see page 81)
Medium DXF (see page 81)
Related products
single donor Pellet
(see page 91)
hMNC-CB single donor Pellet
(see page 91)
hMNC-PB pooled Pellet (see page 91)
hMNC-CB
pooled Pellet (see page 91)

Separation
GM-CSF (see page 179)
M-CSF (see page 179)
TNF-alpha (see page 179)
sRANKL (see page 179)
Lymphocyte
hMNC-PB
Human Stem and

Blood Cells

Cord Blood (hMNC-CB)
57
Product
Size
Catalog Number
C-12907
C-14098
C-12908
C-14099
Human Mononuclear Cells

from Cord Blood (hMNC-CB),
single donor, ultra-pure
C-12901
C-14096

pooled, ultra-pure
C-12904
C-14097

from Peripheral Blood (hMNC-PB),
hMNC-PB single donor Pellet
from Peripheral Blood (hMNC-PB),
pooled, ultra-pure
hMNC-PB pooled Pellet
hMNC-CB pooled Pellet
* These differentiation media are applicable for CFU-assays for hematopoietic progenitor cells (LTC-IC assays) or Hill-assays for endothelial progenitor cells
(Hill et al. 2003, N Engl J Med 348: 593-600).
58
Overview Human Stem and Blood Cells
Human Stem and

Blood Cells
Product
Cat. No.
Cryopr.
Cells
Recommended
Culture Media
Recommended
Differentiation
Media
Recommended
Sera
Plating
Density
Page
Human Mesenchymal Stem

Cells from Bone Marrow
(hMSC-BM)
C-12974
C-28010
C-28019
C-28011
C-28012
C-28013
C-28014
C-28015
C-37385
C-37386
4,000 cells
per cm2
49

Cells from Umbilical Cord
Matrix (hMSC-UC)
C-12971
C-28010
C-28019
C-28011
C-28012
C-28013
C-28014
C-28015
C-37385
C-37386
4,000 cells
per cm2
49

Cells from Adipose Tissue
(hMSC-AT)
C-12977
C-28010
C-28019
C-28011
C-28012
C-28013
C-28014
C-28015
C-37385
C-37386
4,000 cells
per cm2
49
Human Pericytes
from Placenta (hPC-PL)
C-12980
C-28040
C-37385
C-37386
4,000 cells
per cm2
51

from Cord Blood (hCD34+-CB),
single donor
C-12921
C-28021
C-39890
C-28020*
C-28025*
C-37387
C-37388
5,000 cells
per ml
52

pooled
C-12924
C-28021
C-39890
C-28020*
C-28025*
C-37387
C-37388
5,000 cells
per ml
52

single donor
C-12927
C-28021
C-39890
C-28020*
C-37387
C-37388
5,000 cells
per ml
53

from Cord Blood (hCD133+CB), pooled
C-12929
C-28021
C-39890
C-28020*
C-37387
C-37388
5,000 cells
per ml
53

from Peripheral Blood
(hMoCD14+-PB), single donor
C-12909
C-28030
C-37387
C-37388
1 x106 cells
per ml
54

from Peripheral Blood
(hMoCD14+-PB), single donor,
pre-screened
C-12912
C-28030
C-37387
C-37388
1 x106 cells
per ml
54
Human Monocytes from

Peripheral Blood (hMo-PB),
single donor
C-12910
C-28030
C-37387
C-37388
1 x106 cells
per ml
54
Human Monocytes from

Peripheral Blood (hMo-PB),
pre-screened
C-12913
C-28030
C-37387
C-37388
1 x106 cells
per ml
54

from Peripheral Blood (hMNCPB), single donor, ultra-pure
C-12907
C-28030
C-37387
C-37388
1 x106 cells
per ml
56

from Peripheral Blood (hMNCPB), pooled, ultra-pure
C-12908
C-28030
C-37387
C-37388
1 x106 cells
per ml
56

C-12901
C-28030
C-28021
C-39890
C-28020*
C-28025*
C-37387
C-37388
1 x106 cells
per ml
57

pooled, ultra-pure
C-12904
C-28030
C-28021
C-39890
C-28020*
C-28025*
C-37387
C-37388
1 x106 cells
per ml
57
C-28050
C-28050
* These differentiation media are applicable for CFU-assays for hematopoietic progenitor cells (LTC-IC assays) or Hill-assays for endothelial
progenitor cells (Hill et al. 2003, N Engl J Med 348: 593-600).
Media and Sera

High-quality media and sera are vital for successful cell culture.
Therefore, PromoCell adheres to strict quality standards and
produces media and sera only from raw materials from strictly
approved sources. We also have invested a lot of effort in media optimization and have substituted serum by defined substances wherever possible. This ensures effective cell growth
and avoids the unpredictable effects of indeterminate compounds.
Media and Sera

3.1
Media for Primary Cells . . . . . . . . . . . . . . 61
3.1.1
Myocyte Medium . . . . . . . . . . . . . . . . . . . . . 62
3.1.2
Chondrocyte Medium . . . . . . . . . . . . . . . . . . 62
3.1.3
Endothelial Cell Media . . . . . . . . . . . . . . . . . 63
3.1.4
Endothelial Cell Media MV . . . . . . . . . . . . . . 64
3.1.5
Epithelial Cell Media . . . . . . . . . . . . . . . . . . . 65
3.1.6
Fibroblast Media . . . . . . . . . . . . . . . . . . . . . . 70
3.1.7
Follicle Dermal Papilla Cell Medium . . . . . . . 72
3.1.8
Hepatocyte Media . . . . . . . . . . . . . . . . . . . . 73
3.1.9
Keratinocyte Medium . . . . . . . . . . . . . . . . . . 74
3.1.10 Melanocyte Media . . . . . . . . . . . . . . . . . . . . 75

3.1.11 Osteoblast Media . . . . . . . . . . . . . . . . . . . . . 76
3.1.12 Preadipocyte/Adipocyte Media . . . . . . . . . . . 77
3.1.13 Skeletal Muscle Cell Media . . . . . . . . . . . . . . 78
3.1.14 Smooth Muscle Cell Medium . . . . . . . . . . . . 79
3.1.15 Freezing Medium . . . . . . . . . . . . . . . . . . . . . 79
3.2
Media for Stem and Blood Cells . . . . . . . . . 80
3.2.1
Mesenchymal Stem Cell Media . . . . . . . . . . . 80
3.2.2
Pericyte Medium . . . . . . . . . . . . . . . . . . . . . 81
3.2.3
Hematopoietic Progenitor Cell Media . . . . . . 81
3.2.4
Endothelial Progenitor Medium . . . . . . . . . . 82
3.2.5
Dendritic Cell Generation Media . . . . . . . . . . 82
3.2.6
Macrophage Generation Media . . . . . . . . . . 83
3.2.7
Mononuclear Cell Medium . . . . . . . . . . . . . . 84
3.2.8
Blood Cell Separation Medium . . . . . . . . . . . 84
3.3
Classical Media . . . . . . . . . . . . . . . . . . . . . . 85
3.4
Insect Cell Culture Media . . . . . . . . . . . . . . 87
3.5 Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
3 Media and Sera
61
3.1 Media for Primary Cells

convenience or as Medium Kit, which
allows user control over the supplement
type and concentration.
Each medium lot is tested for its ability to
support the growth of the intended primary cells. In addition, biochemical and
Medium (Ready-to-use)
in culture (including L-Glutamine and

NaHCO3).
The shelf life of the Basal Medium is
9 months (at 4 to 8C), that of the
SupplementMix is 12 months (at -20C)
from the date of manufacture. After
adding the SupplementMix, the shelf life
of the complete medium is 6 weeks (at
4 to 8C).
The Medium (Ready-to-use) consists of

a 500 ml bottle with Basal Medium and
one vial with SupplementMix. Adding
the SupplementMix to the Basal Medium
results in the complete medium, which
contains all the growth factors and
supplements necessary for the propagation of the specific human primary cells
Medium Kit
The Medium Kit consists of a 500 ml
bottle with Basal Medium and a SupplementPack. The SupplementPack is a set
of individual vials with pre-measured
supplements. Adding all components of
the SupplementPack to the Basal Medium results in the complete medium,
which contains all the growth factors
sterility tests are performed*.

Note: The media for primary cells do not
contain antibiotics.
Media and Sera
The PromoCell media for primary cells

are formulated for the optimal growth
of specific types of primary human cells
and a variety of primary animal cells.
For most cell types, they are available
as Medium (Ready-to-use) for maximal
and supplements necessary for the propagation of specific human primary cells
in culture (including L-Glutamine and
NaHCO3). Since all supplements can be
added separately, the user has full control over the media formulation and can
adapt it to his/her experimental requirements.
9 months (at 4 to 8C), and that of
the SupplementPack is 12 months
(at -20C) from the date of manufacture. After adding the supplements, the shelf life of the complete
medium is 6 weeks (at 4 to 8C).
Basal Media (with or without phenol
red) as well as SupplementMixes and
SupplementPacks can also be purchased
separately.
If you require special media modifications, we offer a Custom Media Service starting at 10 bottles per order.
Please ask for details.
*Certificates of analysis are available on request.
62
3 Media and Sera
Low Serum
3.1.1 Myocyte Medium
Myocyte Growth Medium
Recommended for
Cardiac Myocytes
(see page 11)
Media and Sera
Human
The PromoCell Myocyte Growth Medium

is a low serum (5% V/V) medium supplemented with well chosen hormones and
growth factors. It was developed for the
optimal expansion and maintenance of
primary cardiac myocytes in culture. The
medium is optimized for primary human

cells but can also be used for primary porcine cardiac myocytes.
Related products
Fetal Calf Serum (see page 87)
Final supplement concentrations

(after addition to the medium)
Fetal Calf Serum
Epidermal Growth Factor
(recombinant human)
0.05 ml / ml
0.5 ng / ml
Basic Fibroblast Growth Factor

(recombinant human)
2 ng / ml
Insulin (recombinant human)
5 g / ml
Product
Size
Catalog Number
Myocyte Growth Medium (Ready-to-use)
500 ml
C-22070
Myocyte Growth Medium Kit
500 ml
C-22170
Myocyte Basal Medium
500 ml
C-22270
Myocyte Basal Medium, phenol red-free
500 ml
C-22275
Myocyte Growth Medium SupplementMix
for 500 ml
C-39275
Myocyte Growth Medium

SupplementPack
for 500 ml
C-39270
3.1.2 Chondrocyte Medium

Chondrocyte Growth Medium
Recommended for
Human
Chondrocytes (see page 12)
The PromoCell Chondrocyte Growth

Medium is designed for the optimal
cultivation of human chondrocytes. The
fetal calf serum in the medium is strictly
pretested for optimal performance.
The medium is optimized for primary

human cells but can also be used for primary porcine and rat chondrocytes.
Final supplement concentration

Fetal Calf Serum
0.1 ml / ml
Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-27101
Chondrocyte Basal Medium
500 ml
C-27111
Chondrocyte Basal Medium,

phenol red-free
500 ml
C-27115
for 500 ml
C-39635

SupplementMix
3 Media and Sera
63
Low Serum

Recommended for
Human
Umbilical Vein Endothelial

Cells (see page 13)
Human Umbilical Artery Endothelial
Cells (see page 14)
Human Pulmonary Artery Endothelial
Cells (see page 16)
Human Saphenous Vein Endothelial
Cells (see page 16)

Fetal Calf Serum
Endothelial Cell Growth Medium and

Endothelial Cell Growth Medium 2 are
low-serum (2% V/V) media optimized
for the cultivation of endothelial cells
from large blood vessels.
Endothelial Cell Growth Medium 2 lacks
Endothelial Cell Growth Supplement
(ECGS, bovine hypothalamic extract)
but contains Insulin-like Growth Factor
(Long R3 IGF) and Vascular Endothelial
Growth Factor (VEGF). Generally, VEGF
leads to higher endothelial cell proliferation in culture. But, because of its mul-
tiple effects on cell metabolism, it may

also interfere with certain experimental
setups. In these cases, we recommend
the use of Endothelial Cell Growth Medium, which contains no VEGF.
The media are optimized for primary
human cells but can also be used for
bovine, porcine, murine, caprine, rabbit,
elephant, dog, and rat endothelial cells
from large vessels as well as for various
endothelial cell lines.
Related products
0.02 ml / ml
0.02 ml / ml
0.004 ml / ml
0.1 ng / ml
5 ng / ml
1 ng / ml
10 ng / ml
Insulin-like Growth Factor (Long R3 IGF)
20 ng / ml
Vascular Endothelial Growth Factor 165

(recombinant human)
0.5 ng / ml

(recombinant human)
(recombinant human)
Ascorbic Acid
Heparin
Hydrocortisone
1 g / ml
90 g / ml
22.5 g / ml
1 g / ml
0.2 g / ml
Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-22010
Endothelial Cell Growth Medium Kit
500 ml
C-22110
Endothelial Cell Basal Medium
500 ml
C-22210
Endothelial Cell Basal Medium,

phenol red-free
500 ml
C-22215

SupplementMix
for 500 ml
C-39215

SupplementPack
for 500 ml
C-39210

(Ready-to-use)
500 ml
C-22011
Endothelial Cell Growth Medium 2 Kit
500 ml
C-22111
Endothelial Cell Basal Medium 2
500 ml
C-22211
Endothelial Cell Basal Medium 2,

phenol red-free
500 ml
C-22216

SupplementMix
for 500 ml
C-39216

SupplementPack
for 500 ml
C-39211
Media and Sera
3.1.3 Endothelial Cell Media
64
3 Media and Sera
Low Serum
3.1.4 Endothelial Cell Media MV
Endothelial Cell Growth
Medium MV
Endothelial Cell Growth
Medium MV2
Media and Sera
Recommended for
Human
Dermal Microvascular
Human Coronary Artery Endothelial
Cells (see page 15)
(see page 15)

Fetal Calf Serum
(recombinant human)
(recombinant human)
Human
Dermal Lymphatic Endothelial

Cells (see page 19)
Cells (see page 18)
PromoCell Endothelial Cell Growth Medium MV and Endothelial Cell Growth
Medium MV2 were developed for the
in vitro cultivation of endothelial cells
from microvascular vessels, the coronary
artery, and the aorta.
lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic
extract) but contains the basic Fibroblast
Growth Factor, the Insulin-like Growth
Factor (Long R3 IGF-1) and the Vascular Endothelial Growth Factor (VEGF). Generally,
VEGF leads to higher endothelial cell proliferation in culture. But, because of its multiple
effects on the cell metabolism, it may also
interfere with certain experimental setups.
In these cases, we recommend the use of
Endothelial Cell Growth Medium MV.
The media are optimized for primary human cells but can also be used for bovine,
porcine, murine, rat, and dog endothelial
cells from microvascular vessels as well
as for various endothelial cell lines.
Related products
Endothelial Cell Growth Medium MV
0.05 ml / ml
0.05 ml / ml
0.004 ml / ml
10 ng / ml
5 ng / ml
10 ng / ml
Insulin-like Growth Factor (Long R3 IGF-1)
20 ng / ml
Vascular Endothelial Growth Factor 165

(recombinant human)
0.5 ng / ml
Ascorbic Acid
1 g / ml
Heparin
Hydrocortisone
90 g / ml
1 g / ml
0.2 g / ml
Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-22020
Endothelial Cell Growth Medium MV Kit
500 ml
C-22120
Endothelial Cell Basal Medium MV
500 ml
C-22220
Endothelial Cell Basal Medium MV,

phenol red-free
500 ml
C-22225

SupplementMix
for 500 ml
C-39225

SupplementPack
for 500 ml
C-39220

(Ready-to-use)
500 ml
C-22022
Endothelial Cell Growth Medium MV2 Kit
500 ml
C-22121
Endothelial Cell Basal Medium MV2
500 ml
C-22221
Endothelial Cell Basal Medium MV2,

phenol red-free
500 ml
C-22226

SupplementMix
for 500 ml
C-39226

SupplementPack
for 500 ml
C-39221
3 Media and Sera
65
Serum-Free
3.1.5 Epithelial Cell Media
Recommended for
Human
Nasal Epithelial Cells

(see page 23)
(see page 24)
Human Tracheal Epithelial Cells
(see page 23)
The PromoCell Airway Epithelial Cell

Growth Medium is a serum-free medium
optimized for the cultivation of epithelial
cells from large air passages.
murine, porcine, equine, and rat airway
epithelial cells.
Media and Sera
Airway Epithelial Cell Growth

Medium

Bovine Pituitary Extract
(recombinant human)
0.004 ml / ml
10 ng / ml
5 g / ml
Hydrocortisone
0.5 g / ml
Epinephrine
0.5 g / ml
Triiodo-L-thyronine
6.7 ng / ml
Transferrin, holo (human)
10 g / ml
Retinoic Acid
0.1 ng / ml
Product
Size
Catalog Number
Airway Epithelial Cell Growth Medium

(Ready-to-use)
500 ml
C-21060
Airway Epithelial Cell Growth Medium Kit
500 ml
C-21160
Airway Epithelial Cell Basal Medium
500 ml
C-21260
Airway Epithelial Cell Basal Medium,

phenol red-free
500 ml
C-21265

SupplementMix
for 500 ml
C-39165

SupplementPack
for 500 ml
C-39160
66
3 Media and Sera
Serum-Free
Small Airway Epithelial Cell
Growth Medium
Recommended for
Small Airway Epithelial Cells
(see page 24)
Media and Sera
Human
PromoCell Small Airway Epithelial Cell

developed for the cultivation of epithelial
cells from the distal respiratory system.
The optimal adjustment of growth factors
in this medium ensures excellent in vitro
growth.
The medium is optimized for primary human cells but can also be used for porcine small airway epithelial cells.

(recombinant human)
0.004 ml / ml
10 ng / ml
5 g / ml
Hydrocortisone
0.5 g / ml
Epinephrine
0.5 g / ml
Triiodo-L-thyronine
6.7 ng / ml
10 g / ml
Retinoic Acid
0.1 ng / ml
Bovine Serum Albumin-Fatty Acid Free

(BSA-FAF)
2.5 mg / ml
Product
Size
Catalog Number
Small Airway Epithelial Cell Growth

500 ml
C-21070

Medium Kit
500 ml
C-21170
Small Airway Epithelial Cell Basal Medium
500 ml
C-21270
Small Airway Epithelial Cell Basal Medium,

phenol red-free
500 ml
C-21275

Medium SupplementMix
for 500 ml
C-39175

Medium SupplementPack
for 500 ml
C-39170
3 Media and Sera
67
Serum-Free
Mammary Epithelial Cell Growth
Medium
Recommended for
The PromoCell Mammary Epithelial Cell

developed for the cultivation of epithelial
cells from the mammary gland.
The medium is optimized for primary human cells but can also be used for rat and
murine mammary epithelial cells as well
as mamma carcinoma cell lines.
Mammary Epithelial Cells

(see page 25)
Media and Sera
Human

(recombinant human)
Hydrocortisone
0.004 ml / ml
10 ng / ml
5 g / ml
0.5 g / ml
Product
Size
Catalog Number
Mammary Epithelial Cell Growth Medium

(Ready-to-use)
500 ml
C-21010

Kit
500 ml
C-21110
Mammary Epithelial Cell Basal Medium
500 ml
C-21210
Mammary Epithelial Cell Basal Medium,

phenol red-free
500 ml
C-21215

SupplementMix
for 500 ml
C-39115

SupplementPack
for 500 ml
C-39110
68
3 Media and Sera
Serum-Free/Low Serum
Medium
Medium 2
Media and Sera
Recommended for
Human
Renal Epithelial Cells

(see page 25)
(see page 26)

Fetal Calf Serum
(recombinant human)
Epinephrine
Hydrocortisone
The PromoCell Renal Epithelial Cell

for the cultivation of epithelial cells from
the kidneys.
The PromoCell Renal Epithelial Cell
Growth Medium 2 is an optimized lowserum (5% V/V) medium with epinephrine. The combination of low serum and
selected growth factors ensures successful
growth in vitro and makes this medium
the first choice for renal epithelial cells.
The media are optimized for primary human cells but can also be used for porcine renal epithelial cells.
Related products
Renal Epithelial Cell Growth Medium
Renal Epithelial Cell Growth Medium 2
0.05 ml / ml
10 ng / ml
10 ng / ml
5 g / ml
5 g / ml
0.5 g / ml
36 ng / ml
36 ng / ml
5 g / ml
5 g / ml
Triiodo-L-thyronine
4 pg / ml
4 pg / ml
Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-26001
Renal Epithelial Cell Growth Medium Kit
500 ml
C-26011
Renal Epithelial Cell Basal Medium
500 ml
C-26021
Renal Epithelial Cell Basal Medium,

phenol red-free
500 ml
C-26022

SupplementMix
for 500 ml
C-39604

SupplementPack
for 500 ml
C-39600

(Ready-to-use)
500 ml
C-26030
Renal Epithelial Cell Growth Medium 2 Kit
500 ml
C-26130
Renal Epithelial Cell Basal Medium 2
500 ml
C-26230
Renal Epithelial Cell Basal Medium 2,

phenol red-free
500 ml
C-26235

SupplementMix
for 500 ml
C-39606

SupplementPack
for 500 ml
C-39605
3 Media and Sera
69
Low Serum
Recommended for
Human
Placental Epithelial Cells

(see page 26)
The PromoCell Placental Epithelial Cell

Growth Medium is a low-serum (5%
V/V) medium optimized for the cultivation of epithelial cells from the amniotic
membrane of the placenta. The combination of low serum und selected growth
factors ensures successful growth in vitro.
Related products
Media and Sera
Placental Epithelial Cell Growth

Medium

Fetal Calf Serum
(recombinant human)
0.05 ml / ml
10 ng / ml
5 g / ml
Epinephrine
0.5 g / ml
Hydrocortisone
36 ng / ml
5 g / ml
Triiodo-L-thyronine
4 pg / ml
Product
Size
Catalog Number
Placental Epithelial Cell Growth Medium

(Ready-to-use)
500 ml
C-26040
Placental Epithelial Cell Growth

Medium Kit
500 ml
C-26140
Placental Epithelial Cell Basal Medium
500 ml
C-26240
Placental Epithelial Cell Basal Medium,

phenol red-free
500 ml
C-26245

SupplementMix
for 500 ml
C-39612

SupplementPack
for 500 ml
C-39610
70
3 Media and Sera
Serum-Free
3.1.6 Fibroblast Media
Recommended for
The PromoCell Fibroblast Growth Medium is a serum-free medium optimized

for the cultivation of neonatal and juvenile fibroblasts.
The medium is optimized for primary human cells but can also be used for rat,
porcine, and bovine fibroblasts as well as
fibroblast cell lines.
Human Dermal Fibroblasts,

juvenile foreskin (see page 27)
Media and Sera
Normal

(recombinant human)
1 ng / ml
5 g / ml
Product
Size
Catalog Number
Fibroblast Growth Medium (Ready-to-use)
500 ml
C-23010
Fibroblast Growth Medium Kit
500 ml
C-23110
Fibroblast Basal Medium
500 ml
C-23210
Fibroblast Basal Medium, phenol red-free
500 ml
C-23215

SupplementMix
for 500 ml
C-39315

SupplementPack
for 500 ml
C-39310
Low Serum
Recommended for
Normal
Human Dermal Fibroblasts,

adult donor (see page 27)
(see page 28)
(see page 28)
Human Uterine Fibroblasts
(see page 29)
Human Villious Mesenchymal
Fibroblasts (see page 29)
PromoCell Fibroblast Growth Medium 2

is a low-serum (2% V/V) medium developed for adult human fibroblasts.
The medium is optimized for primary human cells but can also be used for rat,
porcine, and bovine fibroblasts as well as
fibroblast cell lines.

Fetal Calf Serum
0.02 ml / ml

(recombinant human)
1 ng / ml
5 g / ml
Related products
Calf Serum (see page 87)
Fetal
3 Media and Sera
71
Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-23020
Fibroblast Growth Medium 2 Kit
500 ml
C-23120
Fibroblast Basal Medium 2
500 ml
C-23220
Fibroblast Basal Medium 2,

phenol red-free
500 ml
C-23225

SupplementMix
for 500 ml
C-39325

SupplementPack
for 500 ml
C-39320

Recommended for
Human
Cardiac Fibroblasts
(see page 28)
PromoCell Fibroblast Growth Medium 3

has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF). The medium is optimized
for primary human cells but can also be
used for porcine cardiac fibroblasts.
Related products:
calf serum (see page 87)
Fetal

Fetal Calf Serum
0.1 ml / ml

(recombinant human)
1 ng / ml
5 g / ml
Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-23025
Fibroblast Growth Medium 3 Kit
500 ml
C-23130
Fibroblast Basal Medium 3
500 ml
C-23230
Fibroblast Basal Medium 3,

phenol red-free
500 ml
C-23235

SupplementMix
for 500 ml
C-39345

SupplementPack
for 500 ml
C-39350
Media and Sera
72
3 Media and Sera
Low Serum
3.1.7 Follicle Dermal Papilla Cell Medium
Medium
Recommended for
Follicle Dermal Papilla Cells
(see page 30)
Media and Sera
Human
The PromoCell Follicle Dermal Papilla

Cell Growth Medium is a low-serum
(4% V/V) medium. The combination of
low serum and growth factors leads to
excellent proliferation of human follicle
dermal papilla cells in vitro.
Related products

Fetal Calf Serum
0.04 ml / ml
0.004 ml / ml

(recombinant human)
1 ng / ml
5 g / ml
Product
Size
Catalog Number

500 ml
C-26501

Medium Kit
500 ml
C-26502
Follicle Dermal Papilla Cell Basal Medium
500 ml
C-26500
Follicle Dermal Papilla Cell Basal Medium,

phenol red-free
500 ml
C-26505

for 500 ml
C-39625

Medium SupplementPack
for 500 ml
C-39620
3 Media and Sera
73
Serum-Free

Recommended for
Human
Hepatocytes (see page 31)
The PromoCell Hepatocyte Growth Medium is a serum-free medium developed

for the cultivation of human hepato-

(recombinant human)
cytes. It is supplemented with well chosen growth factors and hormones and
suited for long-term cultivation.
The media are optimized for primary human hepatocytes but can also be used
for porcine hepatocytes.
In situations where the media compounds

of the Hepatocyte Growth Medium interfere with certain experimental setups, we
recommend using the PromoCell Hepatocyte Maintenance Medium. This medium
is developed for short-term cultivation
and contains fewer supplements.
Note: For culturing Hepatocytes,

Collagen type I coated culture vessels
are necessary.
Under standard in vitro cell culture
conditions, mature human hepatocytes
usually do not survive for longer than
10-14 days and do not proliferate.
10 ng / ml
5 g / ml
6 ng / ml
Hydrocortisone
0.5 g / ml
10 g / ml
Ascorbic Acid
Bovine Serum Albumin-Fatty Acid Free
(BSA-FAF)
Dexamethasone
250 g / ml
3.75 mg / ml
400 ng / ml
Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-25010
Hepatocyte Growth Medium Kit
500 ml
C-25110
Hepatocyte Basal Medium
500 ml
C-25210
Hepatocyte Basal Medium,

phenol red-free
500 ml
C-25215

SupplementMix
for 500 ml
C-39642

SupplementPack
for 500 ml
C-39640

(Ready-to-use)
500 ml
C-25020
Hepatocyte Maintenance Medium Kit
500 ml
C-25120

SupplementMix
for 500 ml
C-39652

SupplementPack
for 500 ml
C-39650
Media and Sera
3.1.8 Hepatocyte Media
74
3 Media and Sera
Serum-Free
3.1.9 Keratinocyte Medium
Recommended for
Media and Sera
Normal
Human Epidermal
Keratinocytes, juvenile foreskin,
single donor (see page 32)
Keratinocytes, juvenile foreskin,
pooled (see page 32)
Normal
Human Epidermal
Keratinocytes, adult, single donor
(see page 32)

Keratinocytes, adult, pooled
(see page 32)
PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed
for the cultivation of epidermal keratino-
cytes without feeder cells.

The medium is optimized for primary human cells but can also be used for horse
and porcine keratinocytes, as well as
mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition,
it is suited for rat corneal keratinocytes.
Related products
-Solution (see page 98)
2
CaCl

0.004 ml / ml

(recombinant human)
0.125 ng / ml
5 g / ml
Hydrocortisone
0.33 g / ml
Epinephrine
0.39 g / ml

CaCl2
10 g / ml
0.06 mM
Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-20011
Keratinocyte Growth Medium 2 Kit
500 ml
C-20111
Keratinocyte Basal Medium 2
500 ml
C-20211
Keratinocyte Basal Medium 2,

phenol red-free
500 ml
C-20216

SupplementMix
for 500 ml
C-39016

SupplementPack
for 500 ml
C-39011
3 Media and Sera
75
Serum-Free
3.1.10 Melanocyte Media
Recommended for
Normal
Human Epidermal Melanocytes,

juvenile foreskin (see page 33)
The PromoCell Melanocyte Growth

Medium is a serum-free, PMA (Phorbol
Myristate Acetate) containing medium
optimized for the cultivation of human
melanocytes. Since PMA is a tumor promoting mitogen, it may interfere with
some experimental approaches. In these

cases, we recommend using Melanocyte
Growth Medium M2.
The medium is optimized for primary human cells but can also be used for horse,
porcine, murine, and rat melanocytes.
Media and Sera
Melanocyte Growth Medium

0.004 ml / ml

(recombinant human)
1 ng / ml
5 g / ml
Hydrocortisone
0.5 g / ml
Phorbol Myristate Acetate
10 ng / ml
Product
Size
Catalog Number
Melanocyte Growth Medium (Ready-to-use)
500 ml
C-24010
Melanocyte Growth Medium Kit
500 ml
C-24110
Melanocyte Basal Medium
500 ml
C-24210
Melanocyte Basal Medium, phenol red-free
500 ml
C-24215
Melanocyte Growth Medium SupplementMix
for 500 ml
C-39415
Melanocyte Growth Medium SupplementPack
for 500 ml
C-39410
Serum-Free
Melanocyte Growth Medium M2
Recommended for
Normal
Human Epidermal
Melanocytes, juvenile foreskin,
cultured in M2 Medium (see page 33)
Normal
Human Epidermal
Melanocytes, adult donor, cultured
in M2 Medium (see page 33)
The unique PromoCell Melanocyte
Growth Medium 2 is a serum-free and
PMA (Phorbol Myristate Acetate) free
medium optimized for the cultivation of

human melanocytes.
It is optimized for primary human cells
but can also be used for equine, porcine,
murine, and rat melanocytes. It is also
suitable for human pigment epithelial
cells, neuronal cells and melanoma cells.
List of supplements and their concentrations: confidential

Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-24300

(Ready-to-use), phenol red-free
500 ml
C-24305
Melanocyte Basal Medium M2
500 ml
C-24400
Melanocyte Basal Medium M2,

phenol red-free
500 ml
C-24405
for 500 ml
C-39420

SupplementMix
76
3 Media and Sera
3.1.11 Osteoblast Media

Recommended for
Media and Sera
Human
Osteoblasts (see page 34)
PromoCell Osteoblast Growth Medium

was developed to establish and maintain human osteoblast cultures. The
fetal calf serum in the medium is strictly
pretested for optimal performance.
The media are optimized for primary human cells but can also be used for bovine, murine, and rat osteoblasts as well
as osteoblast cell lines.
Final supplement concentration

Fetal Calf Serum
0.1 ml / ml
Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-27001
Osteoblast Basal Medium
500 ml
C-27010
Osteoblast Basal Medium,

phenol red-free
500 ml
C-27015
for 500 ml
C-39615

SupplementMix

Recommended for
Human
Osteoblasts (see page 34)
PromoCell Osteoblast Mineralization

Medium was developed for the efficient induction of strong mineralization
in human osteoblast cultures. Extensive
extracellular calcium deposits can be ob-
served after 21 days, following an easy

one-step protocol (see page 34, Fig. 3).

Product
Size
Catalog Number
100 ml
C-27020
3 Media and Sera
77
Low Serum/Serum-Free
3.1.12 Preadipocyte/Adipocyte Media
Preadipocyte Differentiation
Medium
Adipocyte Nutrition Medium
Recommended for
Human
White Preadipocytes
(see page 35)
The PromoCell Preadipocyte Growth

Medium is a low-serum (5% V/V) medium optimized for the expansion of human preadipocytes.
The Preadipocyte Differentiation Medium is a serum-free medium, which
induces the differentiation of preadipocytes into mature adipocytes.
The media are optimized for primary human cells but can also be used for murine
preadipocytes/adipocytes.
Related products
Fetal
Media and Sera
The mature adipocytes can be cultivated

using the Adipocyte Nutrition Medium.

Fetal Calf Serum
(recombinant human)
Hydrocortisone
Preadipocyte
Growth Medium
Preadipocyte
Differentiation Medium
Adipocyte
Nutrition Medium
0.05 ml / ml
0.03 ml / ml
0.004 ml / ml
10 ng / ml
1 g / ml
d-Biotin
8 g / ml
8 g / ml
0.5 g / ml
0.5 g / ml
Dexamethasone
400 ng / ml
400 ng / ml
IBMX
44 g / ml
L-Thyroxine
9 ng / ml
Ciglitazone
3 g / ml
90 g / ml
Heparin
Product
Size
Catalog Number
Preadipocyte Growth Medium (Ready-to-use)
500 ml
C-27410
Preadipocyte Growth Medium Kit
500 ml
C-27417
Preadipocyte Basal Medium
500 ml
C-27411
Preadipocyte Basal Medium,

phenol red-free
500 ml
C-27415

SupplementMix
for 500 ml
C-39425

SupplementPack
for 500 ml
C-39427

(Ready-to-use)
500 ml
C-27436
Preadipocyte Differentiation Medium Kit
500 ml
C-27437

SupplementMix
for 500 ml
C-39436

SupplementPack
for 500 ml
C-39437
Adipocyte Nutrition Medium (Ready-to-use)
500 ml
C-27438
Adipocyte Nutrition Medium Kit
500 ml
C-27439
Adipocyte Basal Medium
500 ml
C-27431
Adipocyte Basal Medium, phenol red-free
500 ml
C-27435
Adipocyte Nutrition Medium SupplementMix
for 500 ml
C-39438
Adipocyte Nutrition Medium SupplementPack
for 500 ml
C-39439
78
3 Media and Sera
Low Serum/Serum-Free
3.1.13 Skeletal Muscle Cell Media
Skeletal Muscle Cell Growth
Medium
Medium
Media and Sera
Recommended for
Human
Skeletal Muscle Cells

(see page 36)
The PromoCell Skeletal Muscle Cell

Growth Medium is a low-serum (5%
V/V) medium optimized for the expansion of human skeletal muscle cells.
The media are optimized for primary human cells but can also be used for rat,
murine, and rabbit skeletal muscle cells.
The unique Skeletal Muscle Cell Differentiation Medium is a serum-free

medium that induces the fusion of
myoblasts to myotubes with typical
multinucleated syncytia.
Fetal
Related products


Skeletal Muscle Cell

Growth Medium
Skeletal Muscle Cell

Differentiation Medium
Fetal Calf Serum
0.05 ml / ml
Fetuin (bovine)
50 g / ml

(recombinant human)
10 ng / ml

(recombinant human)
1 ng / ml
10 g / ml
10 g / ml
Dexamethasone
0.4 g / ml
Product
Size
Catalog Number

(Ready-to-use)
500 ml
C-23060
Skeletal Muscle Cell Growth Medium Kit
500 ml
C-23160
Skeletal Muscle Cell Basal Medium
500 ml
C-23260
Skeletal Muscle Cell Basal Medium,

phenol red-free
500 ml
C-23265

SupplementMix
for 500 ml
C-39365

SupplementPack
for 500 ml
C-39360

500 ml
C-23061

for 500 ml
C-39366
3 Media and Sera
79
Low Serum

Medium 2
Recommended for
Human
Umbilical Artery Smooth

Human
Aortic Smooth Muscle Cells

(see page 37)
Cells (see page 40)
(see page 39)
Human Bladder Smooth Muscle Cells
(see page 40)
Human Prostate Smooth Muscle Cells
(see page 41)
(see page 41)
The PromoCell Smooth Muscle Cell

Growth Medium 2 is a low-serum (5%
V/V) medium developed to establish and
maintain smooth muscle cell cultures.
bovine, porcine, murine, dog, and rat
Related products

Fetal Calf Serum
(recombinant human)
0.05 ml / ml
0.5 ng / ml

(recombinant human)
2 ng / ml
5 g / ml
Product
Size
Catalog Number
Smooth Muscle Cell Growth Medium 2

(Ready-to-use)
500 ml
C-22062
Smooth Muscle Cell Growth Medium 2 Kit
500 ml
C-22162
Smooth Muscle Cell Basal Medium 2
500 ml
C-22262
Smooth Muscle Cell Basal Medium 2,

phenol red-free
500 ml
C-22267

SupplementMix
for 500 ml
C-39267

SupplementPack
for 500 ml
C-39262
3.1.15 Freezing Medium

Recommended for
Human
and Animal Primary Cells

Cell Lines
Induced Pluripotent Stem Cells
Embryonic Stem Cells
Established
Serum-Free
The PromoCell Cryo-SFM is an animal
component-free, chemically defined medium with DMSO, methylcellulose, and
other cryoprotective components developed for the cryopreservation of human
and animal primary cells. It ensures high
cell viability, excellent growth rates, and

good attachment of the cells after thawing. The medium is the first choice for all
types of primary cells and cell lines.
Product
Cryo-SFM
Size
Catalog Number
30 ml
125 ml
C-29910
C-29912
Media and Sera
3.1.14 Smooth Muscle Cell Medium
80
3 Media and Sera
Media and Sera
3.2. Media for Stem and Blood Cells

The PromoCell media for stem and blood
cells are formulated for either the optimal growth of specific cell types or to
support the differentiation into the respective mature cells.
Each lot is tested for its ability to support
growth or differentiation, respectively. In
addition, biochemical and sterility tests
are performed*.
The Media (Ready-to-use) consist of a
100 ml, 250 ml, or 500 ml bottle of Basal
Medium and a vial of SupplementMix.
Adding the SupplementMix to the Basal
Medium results in the complete medium.
Some Media are provided with additional Cytokine Mixes to achieve optimal
performance.
9 months (at 4 to 8C), and that of the
SupplementMix is 12 months (at -20C)
from the date of manufacture. After
adding the SupplementMix, the shelf life
of the complete medium is 6 weeks (at
4 to 8C).
Note: The media for stem and blood cells
do not contain antibiotics.
If you require special media modifications we offer a Custom Media Service starting at 10 bottles per order.
Please ask for details.
3.2.1 Mesenchymal Stem Cell Media

Growth Medium
Growth Medium DXF
Differentiation Media
Recommended for
Human
Mesenchymal Stem Cells from

Bone Marrow, Umbilical Cord Matrix,
and Adipose Tissue (see page 49)
The PromoCell Mesenchymal Stem Cell

Growth Medium supports the expansion of multipotent human Mesenchymal Stem Cells (MSC) without inducing
early senescence and differentiation as
observed in standard culture media.
The Mesenchymal Stem Cell Growth Medium DXF allows for the robust proliferation of multipotent MSC in a chemically
defined and xeno-free culture environment. It is devoid of all animal-derived
components and substances of human
origin with Human Serum Albumin as
the only exception. Fibronectin-coated
plates are necessary in conjunction with

this medium.
In addition, PromoCell offers five
media to efficiently induce differentiation
of MSC into adipogenic, chondrogenic,
osteogenic or neurogenic lineages, respectively (see also Fig. 3, page 50).
Related products
(see page 96)
Fibronectin Solution, human
(see page 99)

Product
Size
Catalog Number
Mesenchymal Stem Cell Growth Medium

(Ready-to-use)
500 ml
C-28010

DXF (Ready-to-use)
500 ml
C-28019
Fibronectin Solution, human (1 mg/ml)
5 ml
C-43060

Differentiation Medium (Ready-to-use)
100 ml
C-28011

100 ml
C-28012

Differentiation Medium w/o Inducers
(Ready-to-use)
100 ml
C-28014
Mesenchymal Stem Cell Osteogenic

100 ml
C-28013
Mesenchymal Stem Cell Neurogenic

100 ml
C-28015
*Certificates of analysis are available on request.
3 Media and Sera
81
3.2.2 Pericyte Medium

Pericyte Growth Medium
Recommended for
Human
Pericytes (see page 51)
PromoCell Pericyte Growth Medium has

been developed and optimized for the in
vitro cultivation of human pericytes from
all tissues.
Whereas in standard culture media peri-
cytes tend to grow slowly and induce

early senescence, PromoCell Pericyte
Growth Medium optimally supports cell
proliferation and maintenance of multipotency in these cells.
Product
Pericyte Growth Medium (Ready-to-use)
Size
Catalog Number
500 ml
C-28040
3.2.3 Hematopoietic Progenitor Cell Media

Recommended for
Human
Mononuclear Cells from

Cord Blood (see page 57)
(see page 52)
(see page 53)
Human Hematopoietic Progenitor
Cells
The PromoCell Hematopoietic Progenitor
Cell (HPC) Expansion Medium DXF has
been designed for the maximum expan-
sion of primitive HPCs in a fully defined,

xeno-free culture environment. Due to
the utilization of exclusively synthetic,
recombinant or plant-sourced materials,
this medium is chemically defined and
free of all animal-derived components
and substances of human origin with
Human Serum Albumin as the only
exception.
Note: The medium must be supplemented with additional cytokines, e.g.
Cytokine Mix E (see below). Individual
cytokines can be found on our website
www.promokine.info or page 179.
Cytokine Mix E is a ready-to-use mixture
of growth factors for the HPC Expansion
Medium DXF. The animal component-free,
defined formula contains recombinant human TPO, SCF, flt-3 ligand and IL-3. The
optimized formulation allows vigorous
expansion of hematopoietic progenitor
cells, while maintaining their primitive
phenotype. Differentiation is largely
inhibited.
The PromoCell Hematopoietic Progenitor Medium has been developed
for the expansion of hematopoietic
progenitors by means of differentiation
into more committed lineages of blood
cells. Hence, this progenitor medium
is suitable for CFU-based assays, e.g.
long-term culture initiating cell (LTC-IC)
assays.


Product
Cytokine Mix E
for HPC Expansion Medium DXF
(Ready-to-use)
Size
Catalog Number
500 ml
C-28021
1 ml (sufficient for 100 ml Medium)

5 ml (sufficient for 500 ml Medium)
C-39890
C-39891
100 ml
C-28020
Media and Sera
82
3 Media and Sera
3.2.4 Endothelial Progenitor Medium

Recommended for
Mononuclear Cells
(see page 56)
(see page 52)
Human Endothelial Progenitor Cells
Media and Sera
Human
The PromoCell Endothelial Progenitor

Medium has been developed for the
expansion of endothelial progenitors by
means of differentiation into more committed lineages of endothelial cells.
Hence, this progenitor medium is suitable for the differentiation of primitive
progenitors into mature endothelial cells
as well as for CFU-based assays, e.g. the
Hill-Assay for endothelial progenitor cells (Hill et al. 2003, N Engl J Med
348: 593-600), which is carried out on
fibronectin-coated plates.

Product
(Ready-to-use)
Size
Catalog Number
100 ml
C-28025
3.2.5 Dendritic Cell Generation Media

DC Generation Medium
DC Generation Medium DXF
Monocyte Attachment Medium
Recommended for
Human
Monocytes, freshly isolated

and cryopreserved (see page 54)
Human Mononuclear Cells, freshly
isolated
The PromoCell Dendritic Cell (DC) Generation Media have been developed for the
easy and efficient generation of immature
as well as fully mature myeloid Dendritic
Cells from peripheral blood monocytes.

PromoCell DC Generation Medium allows for the generation of DC from

cryopreserved as well as freshly isolated
cells. In the latter case, the Monocyte Attachment Medium is also needed.
The DC Generation Medium DXF is a
chemically defined and xeno-free formulation for use with freshly isolated
cells. It is free of all animal derived components and substances of human origin
with Human Serum Albumin as the only
exception.
The DC Base Media do not include cytokines and must therefore be supplemented according to the users individual
needs.
The Monocyte Attachment Medium

allows for the efficient adherence selection of Monocytes from freshly isolated Mononuclear Cells (MNC) while
maintaining optimal cell health. Thus,
the time-consuming and costly immunomagnetic purification of monocytes
prior to dendritic cell generation is not
necessary.
Note: See our Instruction Manual and
Application Note for details. Please visit
www.promocell.com/application-notes.
3 Media and Sera
83
Product
Size
Catalog Number
DC Generation Medium (Ready-to-use)
250 ml
C-28050
DC Generation Medium DXF

(Ready-to-use)
250 ml
C-28052
DC Base Medium
250 ml
C-28053
DC Base Medium DXF
250 ml
C-28054

(Ready-to-use)
250 ml
C-28051
3.2.6 Macrophage Generation Media

M1-Macrophage Generation
Medium DXF
M2-Macrophage Generation
Medium DXF
Recommended for
Human
Mononuclear Cells, freshly

isolated
Human Monocytes, freshly isolated
The PromoCell Macrophage Generation Media were developed for
easy and efficient generation of
M1- or M2-polarized macrophages from
peripheral blood monocytes. A detailed
step-by-step protocol is available also,
which allows optional user-customized
macrophage activation.
Completely defined
In combination with the Monocyte

Attachment Medium, freshly isolated
peripheral blood mononuclear cells can
be directly used as a starting material.
The PromoCell M1-Macrophage Generation Medium DXF allows for the
generation of M1-polarized macrophages from freshly isolated cells, whereas the M2-Macrophage Generation
Medium DXF produces M2-polarized
macrophages. The chemically defined
and xeno-free media formulations are
free of all animal derived components
and substances of human origin with
Human Serum Albumin as the only
exception.
The Monocyte Attachment Medium allows for the efficient adherence selection
of Monocytes from freshly isolated
Mononuclear Cells (MNC) while maintaining optimal cell health. Thus, the
time-consuming and costly immunomagnetic purification of monocytes prior
to macrophage differentiation is not
necessary.
Note: See our Instruction Manual and
Application Note for details. Please visit
www.promocell.com/application-notes.
Related products
Detachment Solution
DXF (see page 96)
Macrophage
The Macrophage Base Medium does not

include cytokines and must therefore be
supplemented according to the users
individual needs.

Product
Size
Catalog Number
M1-Macrophage Generation Medium DXF

(Ready-to-use)
250 ml
C-28055

(Ready-to-use)
250 ml
C-28056
Macrophage Base Medium DXF
250 ml
C-28057

(Ready-to-use)
250 ml
C-28051
Media and Sera
84
3 Media and Sera
3.2.7 Mononuclear Cell Medium

Human
Recommended for
The PromoCell Mononuclear Cell Medium is a very rich short-term maintenance medium which provides utmost
cell viability.
Mononuclear Cells
from Cord Blood (see page 57)
from Peripheral Blood (see page 56)
Media and Sera
Human
Monocytes (see page 54)
48 hours before proceeding with your

experiments.
Related products
Lymphocyte Separation Medium
1077 (see below)
It is suitable to maintain MNCs for up to

Product
Mononuclear Cell Medium (Ready-to-use)
Size
Catalog Number
500 ml
C-28030
3.2.8 Blood Cell Separation Medium

Lymphocyte Separation Medium
1077
Recommended for
Peripheral
blood
cord blood
Buffy coats
Apheresis products
Bone marrow
Umbilical
The PromoCell Lymphocyte Separation

Medium 1077 is a sterile, ready-to-use
solution optimized for the separation of
lymphocytes from whole blood or buffy
coats without any loss of viability. It is
based on Ficoll 400 and sodium diatrizoate and provides optimal physiological
parameters. It has a density of 1,077 g/ml
at +20C.
Product
Lymphocyte Separation Medium 1077
Size
Catalog Number
500 ml
C-44010
3 Media and Sera
85
3.3 Classical Media

n DMEM Media
Size
Catalog Number
DMEM, w/o L-Glutamine
500 ml
C-71110
DMEM, with L-Glutamine
500 ml
C-71111
DMEM, with 25 mM HEPES,

w/o L-Glutamine
500 ml
C-71115
DMEM, with 25 mM HEPES,

with L-Glutamine
500 ml
C-71116
DMEM, with stable Glutamine
500 ml
C-71120
DMEM, High Glucose (4.5 g/l),

w/o L-Glutamine
500 ml
C-71210

with L-Glutamine
500 ml
C-71212

with stable Glutamine
500 ml
C-71220

with stable Glutamine, with Na-Pyruvate
500 ml
C-71221

with L-Glutamine, with Na-Pyruvate
500 ml
C-71222

w/o L-Glutamine, with Na-Pyruvate
500 ml
C-71223
n DMEM/Hams F-12 Media

Product
Size
Catalog Number
DMEM/Hams F-12, w/o L-Glutamine
500 ml
C-71310
DMEM/Hams F-12, with L-Glutamine
500 ml
C-71311
DMEM/Hams F-12, with stable Glutamine
500 ml
C-71320
n Hams Media
Product
Size
Catalog Number
Hams F-10, w/o L-Glutamine
500 ml
C-72010
Hams F-10, with L-Glutamine
500 ml
C-72020
Hams F-12, w/o L-Glutamine
500 ml
C-72110
Hams F-12, with L-Glutamine
500 ml
C-72120
n Leibovitz L-15 Medium

Product
Leibovitz L-15, w/o L-Glutamine
Size
Catalog Number
500 ml
C-73110
Media and Sera
Product
86
3 Media and Sera
n McCoys 5A Media
Media and Sera
Product
Size
Catalog Number
McCoys 5A, w/o L-Glutamine
500 ml
C-73210
McCoys 5A, with L-Glutamine
500 ml
C-73211
McCoys 5A, with stable Glutamine
500 ml
C-73220
McCoys 5A, with stable Glutamine,

phenol red-free
500 ml
C-73227
n MEM Alpha and MEM D-Valine Media

Product
Size
Catalog Number
MEM Alpha, w/o Nucleosides,

w/o L-Glutamine
500 ml
C-75012
MEM Alpha, w/o Nucleosides,

with L-Glutamine
500 ml
C-75013
MEM Alpha, with Nucleosides,

with L-Glutamine
500 ml
C-75014
MEM D-Valine, w/o L-Glutamine
500 ml
C-75100
n MEM Earle Media

Product
Size
Catalog Number
MEM Earle, w/o L-Glutamine
500 ml
C-75210
MEM Earle, with L-Glutamine
500 ml
C-75211
MEM Earle, with stable Glutamine
500 ml
C-75220
n RPMI 1640 Media

Product
Size
Catalog Number
RPMI 1640, w/o L-Glutamine
500 ml
C-76010
RPMI 1640, with L-Glutamine
500 ml
C-76011
RPMI 1640, with 25 mM HEPES,

w/o L-Glutamine
500 ml
C-76015
RPMI 1640, with 25 mM HEPES,

with L-Glutamine
500 ml
C-76016
RPMI 1640, w/o L-Glutamine,

phenol red-free
500 ml
C-76017
RPMI 1640, with stable Glutamine
500 ml
C-76020
3 Media and Sera
87
The medium requirements of insect cells

are significantly different from vertebrate
cell culture. For instance, the optimal pH
range for most insect cells is more acidic
(pH 6.2 to 6.5). Also, the amino acid

concentration needs to be higher than in
vertebrate cell media.
Mitsuhashi/Maramorosh Insect
Medium
Mitsuhashi and Maramorosh Insect

Medium is well-suited for Mosquito cell
culture and must be supplemented with
FCS prior to use.
Recommended for
Mosquito
Cells
Product
Mitsuhashi/Maramorosh Insect Medium
Schneiders Drosophila Medium

Recommended for
Dipteran
Size
Catalog Number
500 ml
C-77110
Schneiders Drosophila Medium was

originally developed for the culture of
Drosophila cells but can also be used for
the culture of other dipteran cell lines.
Cells
Product
Schneiders Drosophila Medium,
w/o L-Glutamine
Size
Catalog Number
500 ml
C-77210
3.5 Sera
Fetal Calf Serum
Recommended for
Primary
Human Cells (all types)
The PromoCell Fetal Calf Sera are high

quality, pretested fetal calf serum batches. They are extensively tested for their
ability to support in vitro expansion of
human primary cells.
In addition, histochemical staining is performed to ensure that the cultured primary cells maintain their cell type specific
marker expression.
Product
Fetal Calf Serum (EU Quality)
primary human cell culture tested
Size
Catalog Number
100 ml
500 ml
C-37355
C-37350
Media and Sera
3.4 Insect Cell Culture Media
88
3 Media and Sera
MSC-Qualified Fetal Calf Serum

Recommended for
Mesenchymal
Stem Cells (all types)
The PromoCell MSC-Qualified Fetal Calf

Sera are high quality, pre-selected fetal
calf serum batches. They are extensively
tested for their ability to support in vitro
expansion of human Mesenchymal Stem
Cells. In addition, histochemical staining

is performed to ensure that the cultured
stem cells maintain the ability to differentiate into diverse lineages.
Media and Sera
Fig. 1: Expansion and differentiation of

Mesenchymal Stem Cells (MSC) using
media with the PromoCell MSC-Qualified Fetal Calf Serum. Left: Undifferentiated hMSC-BM cultured for 4 passages
(phase contrast). Right: The same cells
after 14 days of adipogenic induction
(Oil Red O staining of lipid droplets
with Hematoxylin counterstain).
Product
ESC-Qualified Fetal Calf Serum

Recommended for
Pluripotent
Embryonic Stem Cells

Progenitor Cells
Endothelial Progenitor Cells
Hematopoietic
Size
Catalog Number
100 ml
500 ml
C-37386
C-37385
The PromoCell ESC-Qualified Fetal Calf

Sera are high quality, pre-selected fetal
calf serum batches. They are extensively tested for their ability to support
undifferentiated, feeder-free culture of
pluripotent murine embryonic stem cells
in the presence of leukemia inhibitory
factor. The maintenance of pluripotency

is assayed by immunostaining for basal
pluripotency markers. In addition, this
serum is also recommended for the culture of hematopoietic and endothelial
progenitor cells.
Fig. 2: Growth morphology of murine

embryonic stem cells (mESC). Left:
mESC in standard Fetal Calf Serum.
Note the large, flat, differentiated
cells. Right: mESC after 5 passages in
PromoCell ESC-Qualified Fetal Calf
Serum. The cells are small and have a
compact colony morphology typical for
mESC.
Product
Size
Catalog Number
100 ml
500 ml
C-37388
C-37387
Cell Pellets
PromoCell Cell Pellets are an easily accessible source of RNA,
DNA, or proteins from a wide range of human primary cell
types without laborious cell cultivation work. This allows you
to focus on your molecular biology experiments and maximize
your valuable research time.
Cell Pellets
4.1
Cell Pellets . . . . . . . . . . . . . . . . . . . . . . . . . 91
4 Cell Pellets
91
4.1 Cell Pellets

Indefinitely
stable at -20C
with established nucleic
acid purification protocols
Compatible
Gene
expression analysis
characterization studies
Northern blots
Southern blots
Western blots/2D gel electrophoresis
Cell
Features
Available
from all PromoCell

cell types
1 million cells per pellet
Stored in RNAlater
PromoCell Cell Pellets are stored in

RNAlater - an aqueous protection solution
that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins
and protects them against degradation.
This makes the PromoCell Cell Pellets
very useful for a wide range of research
applications* like gene expression analysis, cell characterization studies, and protein characterization experiments.
Note: RNAlater denatures proteins.

Therefore, proteins will be suitable for
e.g. Western blotting but not for applications that require native proteins.
Each Cell Pellet is made from 1 million
cells and is dissolved in 200 l RNAlater.
The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell
type specific antigens. On request the
cells from the same lot are available for
cultivation. The Cell Pellets can be stored
indefinitely at -20C.
Product
Product Description
HAoAF Pellet
Pellet from Human Aortic Adventitial Fibroblasts
C-14037
HAoEC Pellet
Pellet from Human Aortic Endothelial Cells
C-14023
HAoSMC Pellet
Pellet from Human Aortic Smooth Muscle Cells
C-14053
HBdMEC Pellet
Pellet from Human Bladder Microvascular Endothelial Cells
C-14026
HBdSMC Pellet
Pellet from Human Bladder Smooth Muscle Cells
C-14057
HBEpC Pellet
Pellet from Human Bronchial Epithelial Cells
HBMEC Pellet
Pellet from Human Brain Microvascular Endothelial Cells
HBSMC Pellet
Pellet from Human Bronchial Smooth Muscle Cells
C-14055
HCAEC Pellet
Pellet from Human Coronary Artery Endothelial Cells
C-14022
HCASMC Pellet
Pellet from Human Coronary Artery Smooth Muscle Cells
C-14052
HCF Pellet
Pellet from Human Cardiac Fibroblasts
C-14036
HCH Pellet
Pellet from Human Chondrocytes
C-14070
HCM Pellet
Pellet from Human Cardiac Myocytes
C-14080
HCMEC Pellet
Pellet from Human Cardiac Microvascular Endothelial Cells
C-14029
HDBEC adult Pellet
Pellet from Human Dermal Blood Endothelial Cells adult donor
C-14019
HDBEC Pellet
Pellet from Human Dermal Blood Endothelial Cells juvenile foreskin
C-14018
HDLEC adult Pellet
Pellet from Human Dermal Lymphatic Endothelial Cells adult donor
C-14021
HDLEC Pellet
Pellet from Human Dermal Lymphatic Endothelial Cells

juvenile foreskin
C-14020
HDMEC adult Pellet
Pellet from Human Dermal Microvascular Endothelial Cells

adult donor
C-14016
HDMEC Pellet

juvenile foreskin
C-14015

pre-screened
C-14017
HFDPC Pellet
Pellet from Human Follicle Dermal Papilla Cells
C-14005
HHpC Pellet
Pellet from Human Hepatocytes
C-14085
HMEpC Pellet
Pellet from Human Mammary Epithelial Cells
C-14066
hMNC-CB pooled Pellet
Pellet from Human Mononuclear Cells from Cord Blood

pooled, ultra-pure
C-14097
Pellet from Human Mononuclear Cells from Cord Blood

C-14096
hMNC-PB single donor Pellet
Pellet from Human Mononuclear Cells from Peripheral Blood

C-14098
hMNC-PB pooled Pellet
Pellet from Human Mononuclear Cells from Peripheral Blood

pooled, ultra-pure
C-14099
Pellet from Human CD14+ Monocytes from Peripheral Blood,

single donor
C-14110
* More information on RNAlater (FAQs, protocols) at www.invitrogen.com/site/us/en/home/brands/Product-Brand/rnalater.html
Catalog Number
C-14063
C-14032
Cell Pellets
Applications
92
4 Cell Pellets
Cell Pellets
Product
Product Description
Catalog Number

pre-screened Pellet
Pellet from Human CD14+ Monocytes from Peripheral Blood,

single donor pre-screened
C-14112
Pellet from Human Monocytes from Peripheral Blood, single donor
C-14111
hMo-PB single donor

pre-screened Pellet
Pellet from Human Monocytes from Peripheral Blood, single

donor pre-screened
C-14113
hMSC-AT Pellet
Pellet from Human Mesenchymal Stem Cells from Adipose Tissue
C-14092
hMSC-BM Pellet
Pellet from Human Mesenchymal Stem Cells from Bone Marrow
C-14090
hMSC-UC Pellet
Pellet from Human Mesenchymal Stem Cells from Umbilical Cord Matrix
C-14091
HNEpC Pellet
Pellet from Human Nasal Epithelial Cells
C-14062
HOB Pellet
Pellet from Human Osteoblasts
C-14071
HPAEC Pellet
Pellet from Human Pulmonary Artery Endothelial Cells
C-14024
HPASMC Pellet
Pellet from Human Pulmonary Artery Smooth Muscle Cells
C-14051
hPC-PL Pellet
Pellet from Human Pericytes from Placenta
C-14095
HPF Pellet
Pellet from Human Pulmonary Fibroblasts
C-14035
HPlEpC Pellet
Pellet from Human Placental Epithelial Cells
C-14069
HPMEC Pellet
Pellet from Human Pulmonary Microvascular Endothelial Cells
C-14027
HPrSMC Pellet
Pellet from Human Prostate Smooth Muscle Cells
C-14058
HRCEpC Pellet
Pellet from Human Renal Cortical Epithelial Cells
C-14068
HREpC Pellet
Pellet from Human Renal Epithelial Cells
C-14067
HSAEpC Pellet
Pellet from Human Small Airway Epithelial Cells
C-14065
HSaVEC Pellet
Pellet from Human Saphenous Vein Endothelial Cells
C-14025
HTEpC Pellet
Pellet from Human Tracheal Epithelial Cells
C-14064
HTSMC Pellet
Pellet from Human Tracheal Smooth Muscle Cells
C-14056
HUAEC Pellet
Pellet from Human Umbilical Artery Endothelial Cells
C-14013
HUASMC Pellet
Pellet from Human Umbilical Artery Smooth Muscle Cells
C-14050
HUF Pellet
Pellet from Human Uterine Fibroblasts
C-14038
HUtMEC Pellet
Pellet from Human Uterine Microvascular Endothelial Cells
C-14028
HUtSMC Pellet
Pellet from Human Uterine Smooth Muscle Cells
C-14059
Pellet from Human Umbilical Vein Endothelial Cells, single donor
C-14010
HUVEC pooled Pellet
Pellet from Human Umbilical Vein Endothelial Cells, pooled
C-14011
HUVEC Growth Medium 2 single donor

Pellet
Pellet from Human Umbilical Vein Endothelial Cells isolated in

Growth Medium 2, single donor
C-14008
HUVEC Growth Medium 2 pooled

Pellet
Pellet from Human Umbilical Vein Endothelial Cells isolated in

Growth Medium 2, pooled
C-14009
Pellet from Human Umbilical Vein Endothelial Cells pre-screened
C-14012
HVMF Pellet
Pellet from Human Villous Mesenchymal Fibroblasts
C-14034
HWP subcutaneous Pellet
Pellet from Human White Preadipocytes subcutaneous
C-14072
HWP visceral Pellet
Pellet from Human White Preadipocytes visceral
C-14073
NHDF adult Pellet
Pellet from Normal Human Dermal Fibroblasts adult donor
C-14031
NHDF Pellet
Pellet from Normal Human Dermal Fibroblasts juvenile foreskin
C-14030
Pellet from Normal Human Epidermal Keratinocytes adult, single donor
C-14002
NHEK adult pooled Pellet
Pellet from Normal Human Epidermal Keratinocytes adult, pooled
C-14004
NHEK.f single donor Pellet
Pellet from Normal Human Epidermal Keratinocytes

juvenile foreskin, single donor
C-14001
NHEK.f pooled Pellet
Pellet from Normal Human Epidermal Keratinocytes

juvenile foreskin, pooled
C-14003
NHEM M2 adult Pellet
Pellet from Normal Human Epidermal Melanocytes

adult donor, cultured in M2 Medium
C-14043
NHEM.f M2 Pellet

juvenile foreskin, cultured in M2 Medium
C-14042
NHEM.f Pellet

juvenile foreskin
C-14040
SkMC Pellet
Pellet from Human Skeletal Muscle Cells
C-14060

PromoCell offers a broad range of reagents and supplements
for cell culture. This includes enzyme mixtures for tissue and
cell dissociation, buffers for daily cell culture work, antibiotics
and antimycotics in different concentrations, components
to supplement various culture media, such as cytokines and
growth factors, and reagents needed for cell assays. All of these
are intensively tested and meet our stringent quality criteria.

5.1
Detachment Reagents . . . . . . . . . . . . . . . . 95
5.2
Antibiotics and Antimycotics . . . . . . . . . . . . 97
5.3
Buffers and Salt Solutions . . . . . . . . . . . . . . 98
5.4 Supplements . . . . . . . . . . . . . . . . . . . . . . . 99
5 Reagents and Supplements
95
5.1 Detachment Reagents
Recommended for
All
human primary cell types
The PromoCell DetachKit was developed

for gentle and effective detachment of
adherent primary human cells in culture.
Each batch is tested on primary human cells.
The DetachKit is optimized for primary

human cells but can also be used for primary animal cells and cell lines.
The kit contains three components:
HepesBSS (30 mM Hepes)
Trypsin / EDTA Solution
(0.04% / 0.03%)
Trypsin Neutralizing Solution (TNS)
Product
DetachKit
DetachKit 2
The PromoCell DetachKit 2 was developed for very gentle detachment of primary human cells in culture. It contains
less trypsin and EDTA than the standard
DetachKit and is suited for applications,
which require very gentle cell handling.
Size
Catalog Number
30 ml
125 ml
250 ml
C-41200
C-41210
C-41220
The DetachKit 2 is optimized for primary

human cells but can also be used for primary animal cells and cell lines.
The kit contains three components:
(30 mM Hepes)
Trypsin / EDTA Solution
(0.025% / 0.01%)
HepesBSS
Product
DetachKit 2
Accutase-Solution
Recommended for
Primary
cells
lines
Tissue dissociation
Cell
Size
Catalog Number
30 ml
125 ml
250 ml
C-41202
C-41212
C-41222
Accutase is an alternative cell detachment solution to trypsin and can also

be used for tissue dissociation. It was
developed for very gentle and effective
detachment of adherent cells. The well
balanced combination of proteolytic and
collagenolytic activities ensures that sur-
face proteins and epitopes stay entirely

intact. Accutase is therefore perfectly
suited for applications, which require an
unchanged cell surface.
Each batch of the PromoCell AccutaseSolution is tested on primary human
cells.
Product
Accutase-Solution,
Size
Catalog Number
100 ml
C-41310
Reagents and
Supplements
DetachKit
96

Cell Dissociation Solution ACF is a proprietary non-enzymatic reagent for de-
tachment of adherent cell types from

tissue culture plastic or glass surfaces.
Due to its gentle yet effective action it
presents a good animal component-free
alternative to trypsin and other enzymatic

detachment solutions.
Product
Reagents and
Supplements
Macrophage Detachment Solution DXF

The Macrophage Detachment Solution DXF is a non-enzymatic reagent
Size
Catalog Number
100 ml
C-41320
especially optimized for the gentle

detachment of strongly adherent macrophages.
Product
Trypsin Solutions
Trypsin is still the most widely employed
enzyme used for cell detachment.
Size
Catalog Number
250 ml
C-41330
PromoCells Trypsin Solutions exhibit

standardized enzyme activity and contain trypsin from extra-pure lots.
Product
Size
Catalog Number
Trypsin / EDTA (0.04% / 0.03%)
30 ml
125 ml
250 ml
C-41000
C-41010
C-41020
Trypsin / EDTA 2 (0.025% / 0.01%)
30 ml
125 ml
250 ml
C-41002
C-41012
C-41022
Trypsin-EDTA PBS 1:250 (0.05% / 0.02%),

w/o Ca++/Mg++
100 ml
C-41060
Trypsin-EDTA PBS 1:250, (0.5% / 0.2%),

w/o Ca++/Mg++, 10x conc.
100 ml
C-41070
Trypsin Neutralizing Solution
Product
(0.05% Trypsin Inhibitor in 0.1% BSA)
Size
Catalog Number
30 ml
125 ml
250 ml
C-41100
C-41110
C-41120
97
5.2 Antibiotics and Antimycotics

However, antibiotics may not only harm
contaminating organisms, but can also
unfavorably alter the physiology and
lifespan of primary human cells. Therefore PromoCell does not recommend
the routine use of antibiotics in cell
n Antibiotics and Antimycotics

Product
culture as a general preventive measure.

As a further application antibiotics may
be utilized in genetic selection procedures, e.g. G-418.
Size
Catalog Number
Pen/Strep Solution
(10,000 IU/ml / 10 mg/ml)
100 ml
C-42010
Pen/Strep/Fungizone Solution
(10,000 IU/ml / 10,000 g/ml / 25 g/ml)
100 ml
C-42020
Amphotericin B Solution (0.25 mg/ml)
100 ml
C-42040
Gentamicin Solution (5 mg/ml)
100 ml
C-42060
n Selection Antibiotics
Product
Size
Catalog Number
G-418 Sulphate Solution (50 mg/ml)
20 ml
C-42094
Hygromycin B Solution (50 mg/ml)
20 ml
C-42095
Reagents and
Supplements
Antibiotics are used in order to prevent

microbial contaminations in cell culture
during initial isolation steps or to cure
precious cultures exhibiting microbial
contamination, e.g. with mycoplasma or
yeast/fungi.
98
5.3 Buffers and Salt Solutions

Buffers and Salt Solutions are frequently
used in tissue culture, cell isolation procedures, and molecular biology.
For convenience, PromoCell offers

ready-to-use Buffers and Salt Solutions.
They are prepared from raw materials of
n Dulbeccos Phosphate Buffered Saline
Reagents and
Supplements
Product
Dulbeccos PBS
the highest quality using tissue culture

grade water.
Size
Catalog Number
500 ml
C-40230
Dulbeccos PBS, w/o Ca /Mg
500 ml
C-40232
Dulbeccos PBS, w/o Ca++/Mg++, 10x conc.
500 ml
C-40238
++
++
n Hanks Balanced Salt Solutions

Product
Hanks BSS
Size
Catalog Number
500 ml
C-40310
++
Hanks BSS, w/o Ca /Mg
500 ml
C-40320
Hanks BSS, phenol red-free
500 ml
C-40370
Hanks BSS, w/o Ca /Mg ,

phenol red-free
500 ml
C-40390
++
++
++
n Endotoxin-free Buffers and Water

Product
Size
Catalog Number
PBS, endotoxin-free
500 ml
C-40240
PBS, w/o Ca /Mg , endotoxin-free
500 ml
C-40242
TC Water
500 ml
C-49998
++
++
n Other Solutions
Product
Size
Catalog Number
CaCl2-Solution (0.5 M)
2 ml
C-34006
HEPES Buffered Saline (30 mM HEPES)
30 ml
125 ml
250 ml
C-40000
C-40010
C-40020
HEPES Buffer (1 M)
100 ml
C-40030
Sodium bicarbonate Solution (7.5%)
100 ml
C-42340
99
5.4 Supplements
n Growth Supplements
Size
Catalog Number
Endothelial Cell Growth Supplement*

(bovine hypothalamic extract)
2 ml
10 x 2 ml
C-30160
C-30180
Endothelial Cell Growth Supplement/

Heparin* (bovine hypothalamic extract)
2 ml
10 x 2 ml
C-30120
C-30140
Bovine Pituitary Extract 15*

(7.5 mg protein/ml)
2 ml
10 x 2 ml
C-30020
C-30040
Bovine Pituitary Extract 26*

(13 mg protein/ml)
2 ml
10 x 2 ml
C-30021
C-30041
50 mg
C-52310
Human Transferrin APO, low iron content
1g
C-52322
Human Transferrin HOLO, iron saturated
1g
C-52332
Human Insulin,
n Amino Acid Solutions

Product
Size
Catalog Number
L-Glutamine Solution
100 ml
C-42210
Stable Glutamine Solution
100 ml
C-42215
MEM Amino Acids Solution
100 ml
C-42320
MEM NEAA Solution
100 ml
C-42322
n Vitamin and Trace Element Solutions

Product
Size
Catalog Number
MEM Vitamin Solution
100 ml
C-42328
Trace Element Mix (1000x)
100 ml
C-42331
n Extracellular Matrix Proteins
Product
Size
Catalog Number
Fibronectin Solution, bovine (1 mg/ml)
5 ml
C-43050
Fibronectin Solution, human (1 mg/ml)
5 ml
C-43060
* Endothelial Cell Growth Supplement (ECGS) and Bovine Pituitary Extract (BPE) are popular growth supplements in primary cell culture. PromoCell ECGS
and BPE are manufactured from raw material which is exclusively collected in New Zealand to ensure maximum BSE-safety.
Reagents and
Supplements
Product
Cell Analysis
The specific analysis of cellular processes and conditions has
played an increasingly vital role in cell biology. Monitoring
reporter gene expression in cells, determining cell viability and
proliferation, testing for cytotoxicity, and distinguishing live
and dead cells are important procedures in most cell biology
labs. Measuring intracellular signaling and assaying distinct
cell metabolic activities have also become routine. These
applications all require reliable, sophisticated, and user-friendly
research tools.
3
6
Cell Analysis
6.1 Cell Viability and Proliferation Kits . . . . . . 103
6.2 Live/Dead Cell Staining Kits . . . . . . . . . . . 105
6.3 Cytotoxicity Kits . . . . . . . . . . . . . . . . . . . . 106
6.4 Senescence Detection Kit . . . . . . . . . . . . . 106
6.5 Cell-Based Reporter Assays . . . . . . . . . . . 107
6.6 Cell Staining Reagents and

Related Products . . . . . . . . . . . . . . . . . . . 109
6.7 Intracellular Indicators and

Related Products . . . . . . . . . . . . . . . . . . . 115
6.8
Cell Signaling Assays . . . . . . . . . . . . . . . . 119
6.9
Cell Stress and Metabolism Kits . . . . . . . . 120
6.10 Cell Fractionation and

Protein Extraction Kits . . . . . . . . . . . . . . . 122
6.11 Protein Size Markers and

Protein Gel Stains . . . . . . . . . . . . . . . . . . . 123
6.12 Nucleic Acid Isolation Kits . . . . . . . . . . . . . 124
6.13 DNA Size Markers and DNA Gel Stains . . . 125
6 Cell Analysis
103
Applications

Cell viability and proliferation assays

Growth factor/cytokine assays
Optimization of cell culture conditions
Cell number determination
Features
Non-radioactive method
Easy and fast procedure
Reliable and reproducible results
Appropriate for various multiwell
formats and automation

PromoKine offers several types of cell
viability kits: The Fluorometric Cell
Viability Kits I and II; the Colorimetric
Cell Viability Kits I, II, III, and IV; and the
Bioluminescent Cell Viability Kits I and II.
Because all of the kits are designed for
use with various multiwell formats, they
are also suited for automation.
Fluorometric Cell Viability Kit I

(Resazurin)
Fluorometric Cell Viability Kit II

(Calcein)
The resazurin-based PromoKine Cell

Viability Kit I provides a simple and rapid
measurement of cell viability. Moreover,
this single-step, homogenous cell proliferation assay is sensitive, safe, and costeffective. It only requires as few as 80
cells for reproducible results. The assay is
based on the ability of viable cells to convert the non-fluorescent, blue redox dye
Resazurin (also known as Alamar Blue)
into the highly red fluorescent, pink dye
resorufin.The fluorescent or colorimetric
signal generated from the assay is proportional to the number of living cells in
the sample. The kit reagents are nontoxic to cells and allow kinetic monitoring.
While fluorometric resorufin quantitation is more sensitive, colorimetric measurement is also possible.
The PromoKine Fluorometric Cell Viability Kit II is intended for the reliable
fluorometric determination of viable cell
numbers. It utilizes the cell membranepermeable, non-fluorescent dye CalceinAM that is hydrolyzed by esterases into
the cell membrane-impermeable greenfluorescent Calcein (Fig. 1). Since esterases are only active in viable cells, the
amount of Calcein-AM hydrolyzed by
cellular esterases is directly proportional
to the number of viable cells in culture.
The kit has a detection range from less
than 50 to more than 25,000 cells per
well.
The Colorimetric Cell Viability Kits I and

II allow for easy and reliable colorimetric determination of viable cell numbers
with excellent sensitivity and linearity. The kits utilize the tetrazolium salts
WST-8 (Kit I) and WST-1 (Kit II) that are
reduced to water-soluble, orange formazan dyes by dehydrogenases present in
viable cells (see Fig. 2). The absorbance
of the formazan dye is proportional to
the number of metabolically active cells
and can be measured directly from a 96well plate without additional processing.
Calcein-AM
R: acetoxymethyl
Esterase
Viable cell
Colorimetric Cell Viability Kits I

(WST-8) and II (WST-1)
Calcein
Colorimetric Cell Viability Kits III

(XTT) and IV (MTT)
Fig. 1: Assay mechanism of Calcein-AM conversion by intracellular esterases.
WST-8 Formazan
orange dye
WST-8
slightly yellow
1-Methoxy PMS
Viable cell
NADH, NADPH
Dehydrogenase
NAD, NADP
Fig. 2: Assay mechanism of WST-8 conversion by dehydrogenases in viable cells.
The PromoKine Colorimetric Cell Viability Kits III and IV were developed for
determining the number of viable cells in
culture. They are based on the reduction
of the tetrazolium salts XTT or MTT into
colored formazan compounds by mitochondrial enzymes. Since these enzymes
are inactivated shortly after cell death,
this is a reliable method for the detection of viable cells. The absorbance of
the formazan product can be measured
directly from a 96-well plate without additional processing.
Cell Analysis
6.1 Cell Viability and Proliferation Kits
Cell Analysis
104
6 Cell Analysis
Bioluminescent Cell Viability Kit I

(ATP)
Bioluminescent Cell Viability Kit II

(ADP/ATP)
The Promokine Bioluminescent Cell

Viability Kit I employs bioluminescence
to measure the ATP level as a marker for
cell viability in mammalian cells. It utilizes
luciferase to catalyze the ATP dependent, light-producing oxidation of luciferin. This light formation can be detected
using a luminometer or beta counter.
The assay can be performed directly in
culture plates requiring no harvesting,
washing, or sample preparation and can
be fully automated for high throughput
(10 seconds/sample). It is highly sensitive, detecting 10-100 mammalian cells
per well. It also offers applications besides cell viability testing, e.g., detecting low level bacterial contamination in
blood, milk, urine, soil, and sludge.
The ADP/ATP ratio is a good indicator for

cell viability. High ATP and low ADP levels are usually found in proliferating cells.
In contrast, decreased levels of ATP and
increased levels of ADP are characteristic for apoptotic cells. In necrotic cells,
the ADP levels are even higher, and ATP
can be barely detected. The Bioluminescent Cell Viability Kit II utilizes bioluminescence to measure the ADP/ATP ratio
providing an innovative system for fast,
simultaneous screening of cell proliferation, apoptosis, necrosis, and growth arrest in mammalian cells. The ATP level is
determined using the luciferin-luciferase
reaction used in the Bioluminescent Cell
Viability Kit I. The ADP level is then measured by its conversion to ATP, which is
subsequently detected using the same
luciferase-catalyzed reaction. The assay works with full automation for high
throughput, detects as few as 100 mammalian cells per well, and offers highly
consistent results with excellent correlation to other apoptosis markers (e.g.,
TUNEL-based or caspase assays).
Cell Proliferation Kit I (CFSE)

PromoKines Cell Proliferation Kit I
provides a convenient and sensitive
way to determine cell proliferation or
cell division. The assay is based on the
amine-reactive dye CFDA SE (5-(and
6)-carboxyfluorescein diacetate, succinimidyl ester; also known as CFSE) which
is non-fluorescent and can diffuse passively into cells. When it enters the cells,
the acetate group is removed by intracellular esterases yielding the bright greenfluorescent dye carboxyfluorescein SE.
Its succinimidyl ester (SE) reactive group
reacts with intracellular amines and attaches the fluorescent dye to intracellular
proteins, retaining it in the cells and allowing fixation with aldehyde fixatives.
CFDA SE has been used as a tracer for
several cell divisions as the label is stably
inherited by daughter cells through successive cell divisions or after cell fusion
and is not transferred to adjacent cells in
a population.
The kit is compatible with flow cytometry and microscopy and contains 10
single-use vials as well as a detailed protocol for cellular labeling.
https://www.promokine.info/shop
Product
Size
Catalog Number
500 assays
2500 assays
10000 assays
PK-CA707-30025-0
PK-CA707-30025-1
PK-CA707-30025-2
1000 assays
PK-CA707-30026
Colorimetric Cell Viability Kit I (WST-8)
100 assays
500 assays
PK-CA705-CK04-100
PK-CA705-CK04
Colorimetric Cell Viability Kit II (WST-1)
500 assays
2500 assays
PK-CA577-K304-500
PK-CA577-K304-2500
Colorimetric Cell Viability Kit III (XTT)
200 assays
1000 assays
PK-CA20-300-200
PK-CA20-300-1000
Colorimetric Cell Viability Kit IV (MTT)
200 assays
1000 assays
PK-CA707-30006-200
PK-CA707-30006
Bioluminescent Cell Viability Kit I (ATP)
200 assays
1000 assays
PK-CA577-K254-200
PK-CA577-K254-1000
Bioluminescent Cell Viability Kit II (ADP/ATP)
200 assays
PK-CA577-K255
10 x 50 g
PK-CA707-30050
10 mg
PK-CA707-10054
Fluorometric Cell Viability Kit I (Resazurin)
Fluorometric Cell Viability Kit II (Calcein)
Resazurin, sodium salt
Note: Please check our website regularly for new cell-based assays.
6 Cell Analysis
105
6.2 Live/Dead Cell Staining Kits
Monitoring of viable and dead cells

Cell counting by fluorescence micros copy and fluor cytometry
Features

Simultaneous monitoring of viable

and dead cells
High fluorescent intensity
Fast and convenient procedure
Excitation and emission spectra are
compatible with common excitation
sources and filter sets
Live/Dead Cell Staining Kit II

The Live/Dead Cell Staining Kit II utilizes
two fluorescent dyes, Calcein-AM and
Calcein-AM
R: acetoxymethyl
Ethidium homodimer III (EthD-III). Calcein-AM penetrates the cell membrane

of living cells. Once inside, the cellular
esterases present in living cells hydrolyze it to cell membrane-impermeable,
green-fluorescent Calcein (emission
maximum at 515 nm).
In contrast, EthD-III can only pass
through the disrupted membrane areas
of dead cells where it intercalates with
the DNA of the nucleus, emitting red
light at 620 nm. Compared with PI,
EthD-III is more stable and emits brighter
fluorescent signals. Since both Calcein
and EthD-III can be excited at 490 nm,
a simultaneous monitoring of viable and
dead cells is possible using a fluorescence
microscope (see Fig. 4). At an excitation
of 545 nm, the number of dead cells can
be monitored.
Bacteria Live/Dead Staining Kit

The Bacteria Live/Dead Staining Kit enables a two-color fluorescent staining
on both live bacteria (green) and dead
bacteria (red) using the two nucleic
acid dyes DMAO and EthD-III. DMAO
is a green-fluorescent dye which stains
both live and dead bacteria with intact
and damaged cell membranes while
the red-fluorescing EthD-III only stains
dead bacteria with damaged cell membranes. With an appropriate mixture of
both dyes, viable bacteria with intact cell
membranes are stained green, whereas
bacteria with damaged cell membranes
fluoresce red. The kit is applicable to
most bacteria types for viability and
cytotoxicity studies and is suitable for
fluorescence microscopy and flow cytometry.
EthD-III
Esterase
Viable cell
DNA intercalation
red fluorescence
Fig. 4: Double-staining of viable

(green) and dead (red) cells using the
PromoKine Live/Dead Cell Staining
Kit II.
Calcein
green fluorescence
Fig. 3: Assay mechanism of the PromoKine Live/Dead Cell Staining Kit II.
Product
Size
Catalog Number
300-1000 assays
PK-CA707-30002
100-1000 assays
PK-CA707-30027
Please see also the PromoKine Apoptotic/Necrotic/Healthy Cells Detection Kit on page 140 and our individual stains for viable
and dead cells on page 110.
Cell Analysis
Applications
106
6 Cell Analysis
6.3 Cytotoxicity Kits

Applications
Cytotoxicity assays
Chemosensitivity testing
Toxicity screening
Cell Analysis
Features

Highly sensitive
Compatible with common plate readers
It oxidizes lactate to pyruvate, triggering

the conversion of the yellow tetrazolium
salt WST into the water-soluble red
formazan dye.
The increase in the amount of formazan
produced in culture medium can be
analyzed spectrophotometrically and
correlates directly to the increase in the
number of lysed cells.
The kit can be used for many different in
vitro cell systems to quantify cytotoxicity
and cytolysis.
LDH Cytotoxicity Kit II
AK Cytotoxicity Kit
The colorimetric LDH Cytotoxicity Kit

II provides a fast, sensitive, and precise
alternative to the radioactive [3H]-thymidine and [51Cr] release assays. It is based
on the activity of lactate dehydrogenase
(LDH), a stable cytoplasmic enzyme that
is present in all cells. Damaged cells
rapidly release this enzyme into the surrounding medium. LDH activity is determined by a coupled enzymatic reaction.
The bioluminometric AK Cytotoxicity Kit

is based on the measurement of adenylLDH
Lactate
NAD+
Formazan
(red)
ate kinase (AK), a ubiquitous protein

present in all cells that is rapidly released
into the culture medium upon damage
to the plasma membrane. The simple
one-step procedure involves two chemical reactions. The adenylate kinase release from the damaged cells catalyses
the conversion of ADP to ATP. The luciferase catalyses a light-producing reaction between the ATP, oxygen, and luciferin. The light can be measured using a
luminometer or beta counter. This highly
sensitive assay allows the rapid quantitation of plasma membrane damage for
evaluation of cell death or cytotoxicity
events and it is well suited for automation and high throughput.
Pyruvate
NADH + H+
Catalyst
WST-1
(yellow)
Fig. 5: Assay mechanism of the PromoKine LDH Cytotoxicity Kit II.

Product
Size
Catalog Number
500 assays
PK-CA577-K313
AK Cytotoxicity Kit
500 assays
PK-CA577-K312
6.4 Senescence Detection Kit

Applications
Histochemical detection of senescent
cells
Cell senescence and oncology studies
Features
High sensitivity and specificity
Compatible with common lab
equipment
Senescence is an arrested state in which

the cells remain viable but are not stimulated to divide by serum or passage in
culture. It is thought to be a tumor-suppressive mechanism and an underlying
cause of aging. Senescent cells display an
increase in size, senescence-associated
expression of b-galactosidase (SA-b-Gal)
activity, and altered patterns of gene
expression. The PromoKine Senescence
Detection Kit has been designed to histochemically detect a specific senescence

marker (SA-b-Gal activity) in cultured
cells and tissue sections.
The specific histochemical marker is
only present in senescent cells and is not
found in presenescent, quiescent, or immortal cells.
Product
Senescence Detection Kit
Size
Catalog Number
250 assays
PK-CA577-K320
6 Cell Analysis
107
6.5 Cell-Based Reporter Assays
Monitoring and quantification of

reporter gene expression (Luciferase,
b-Galactosidase, GFP)
Determination of transfection
efficiencies
Features

Highly sensitive and reproducible

Fast and convenient protocols
Compatible with common
plate readers
under these conditions, light emitted

from the reaction is directly proportional
to the number of luciferase enzyme
molecules. The light emission from the
luciferase reaction can be recorded using
a luminometer.
The Luciferase Reporter HTS Kits have
been specifically designed for economic
high-throughput applications using
high-density microplates.
In addition to the Firefly luciferase substrate D-Luciferin, we also provide a
wide range of coelenterazine substrates
for the Renilla luciferase catalysed reaction.
Luciferase Reporter Assay Kits

GFP Reporter Assay Kit
The PromoKine ready-to-use Luciferase
Reporter Assays provide complete systems for rapid, convenient, and quantitative detection of luciferase activity in
eukaryotic cells, e.g. in cells transfected
with plasmids carrying the Firefly and/
or Renilla luciferase reporter genes. They
are optimized to provide excellent sensitivity as well as consistent light output
when working with multiple samples.
Firefly luciferase catalyzes the oxidative
carboxylation of luciferin, a reaction
with the highest efficiency of any known
bioluminescence reaction. Consequently,
this sensitive reporter is ideally suited for
studying gene regulation and function.
Renilla luciferase is often used in conjunction with Firefly luciferase as a
normalizing transfection control or for
multiplexing transcriptional reporter assays. Both Firefly luciferase and Renilla
luciferase show maximum light output
when substrates and co-factors are present in large quantities. When assayed
GFP (Green Fluorescent Protein) is a fluorescent protein originally isolated from

the jellyfish Aequorea victoria. GFP and
its enhanced variant EGFP have been
widely used as reporter proteins for in
vivo monitoring of gene expression in a
variety of cell types and organisms. Since
GFP requires no additional substrates or
cofactors, GFP fluorescence can easily
be detected using a fluorescence microscope. However, most imaging studies of GFP are qualitative. Quantitative
analyses of GFP expression levels in cells
or tissues are more informative and have
wider applications.
The PromoKine GFP Reporter Assay Kit
was designed for use in a 96-well microplate format (Ex/Em = 488 nm/507nm).
Cells or tissues can be homogenized directly in the provided GFP assay buffer
and the supernatant of the lysed cells/
tissues is transfered to the microwell
plates. The amount of GFP is determined
by comparing its fluorescence with that

of a GFP standard. The kit can detect a
wide range of GFP concentrations (0.0110 g/ml). Furthermore, a GFP quench
solution is provided for determining
autofluorescence levels of cell or tissue
extracts.
Beta-Galactosidase Kits
The b-Galactosidase Staining Kit and
the b-Galactosidase Reporter Assay Kit
were designed to detect and quantify
b-Gal activity in cells transfected with
a plasmid carrying the frequently-used
LacZ reporter gene. This gene encodes
b-galactosidase, an enzyme that is very
stable and resistant to proteolytic degradation. When expressed in transfected
cells, it converts a colorless substrate
(e.g., CPRG, X-Gal, or ONPG) into a
colored reaction product that can be
easily detected microscopically (b-Galactosidase Staining Kit) or quantified using
a spectrophotometer (b-Galactosidase
Reporter Assay Kit). In addition, PromoKine provides a range of chromogenic
beta-Galactosidase substrates.
Fig. 6: b-Gal staining of CHO-K1 cells

transfected with the pPK-CMV-R1 reporter vector expressing the lacZ gene
(see page 166).
Product
Size
Catalog Number
Luciferase Reporter Assay Kit I (Firefly)
150 assays
1000 assays
PK-CA707-30003-1
PK-CA707-30003-2
Luciferase Reporter Assay Kit II (Renilla)
150 assays
1000 assays
PK-CA707-30004-1
PK-CA707-30004-2
Luciferase Reporter Assay Kit III

(Firefly & Renilla)
100 assays
1000 assays
PK-CA707-30005-1
PK-CA707-30005-2
Luciferase Reporter HTS Kit (Firefly)
4 ml
10 ml
100 ml
10 x 100 ml
PK-CA707-30028-0
PK-CA707-30028-1
PK-CA707-30028-2
PK-CA707-30028-3
Cell Analysis
Applications
108
6 Cell Analysis
Product
Size
Catalog Number
beta-Galactosidase Reporter Assay Kit
500 assays
PK-CA708-10100
beta-Galactosidase Staining Kit
200 assays
PK-CA577-K802
GFP Reporter Assay Kit
100 assays
PK-CA577-K815
D-Luciferin, Free Acid
10 mg
50 mg
1g
PK-CA707-10100
PK-CA707-10100-1
PK-CA707-10100-2
D-Luciferin, Potassium Salt
10 mg
50 mg
1g
PK-CA707-10101
PK-CA707-10101-1
PK-CA707-10101-2
D-Luciferin, Sodium Salt
10 mg
50 mg
1g
PK-CA707-10102
PK-CA707-10102-1
PK-CA707-10102-2
5 mg
PK-CA707-10103
Coelenterazine (native)
50 g
PK-CA707-10110
Coelenterazine h
50 g
PK-CA707-10111
Coelenterazine cp
50 g
PK-CA707-10112
Coelenterazine hcp
50 g
PK-CA707-10113
Coelenterazine f
50 g
PK-CA707-10114
Coelenterazine n
50 g
PK-CA707-10115
Coelenterazine ip
50 g
PK-CA707-10116
Coelenterazine fcp
50 g
PK-CA707-10117
Coelenterazine i
50 g
PK-CA707-10121
Coelenterazine, 2-methyl
50 g
PK-CA707-10122
Coelenterazine 400a
(also known as DeepBlue C,
a trademark of Perkin Elmer)
50 g
PK-CA707-10125
1 Kit
PK-CA707-10123
X-Gal
100 mg
1g
5g
PK-CA707-10011
PK-CA707-10011-1
PK-CA707-10011-2
Red-beta-D-Gal
100 mg
PK-CA707-10012
Rose-beta-D-Gal
100 mg
PK-CA707-10013
25 mg
PK-CA707-10014
Cell Analysis
Firefly Luciferase Substrates
DMNPE-caged D-Luciferin
Renilla Luciferase Substrates
Coelenterazine Sampler Kit

Chromogenic beta-Galactosidase Substrates
Purple-beta-D-Gal
Green-beta-D-Gal
Chromogenic beta-Galactosidase
Substrate Sampler Kit
25 mg
PK-CA707-10015
1 kit (5 x 5 mg)
PK-CA707-10024
Please see page 166 and www.promokine.info for luciferase, beta-Gal, and GFP reporter vectors.
6 Cell Analysis
109
6.6 Cell Staining Reagents and Related Products
Detection of cellular structures

Fluorescence microscopy, Flow
cytometry
Features
High quality
Thoroughly tested
Highly sensitive
Fluorescent dyes offer extraordinary sensitivity and extended possibilities for the
visualization and analysis of intracellular
processes and cell structures such as the
cell membrane, mitochondria, the nucleus, and the cytoskeleton. Some dyes
are also suited for staining only viable

or dead cells. That is an important feature for elucidating the effects of certain
agents on cell viability.
PromoKine provides a select choice of
reagents for staining different cell organelles and structures as well as for
determining processes like membrane
potential changes. Some of these reagents are available as esters, which pass
through the membranes of viable cells.
Once inside, the ester group is hydrolyzed by cellular esterases, and the dye
can accumulate in the cell. Some dyes
are nonfluorescent in their ester form but
fluoresce strongly when the ester group
has been removed.
Fig. 7: Primary human fibroblasts (NHDF,

see page 27) stained with Calcein and
counterstained with DAPI.
Product
Product Description
Size
Catalog Number
1 mg
PK-CA707-40037
10 ml
(10 mg/ml)
PK-CA707-40039
10 mg
PK-CA707-40011
10 mg in 1 ml
PK-CA707-40043
10 mg
PK-CA707-40009
Nucleus Stains
7-AAD
(7-aminoactinomycin D)
Fluorescent DNA intercalator
Acridine Orange (AO)

Solution
Nucleus stain (DNA/RNA)
DAPI
Nucleus stain (DNA)
DAPI Solution
Nucleus stain (DNA); aqueous solution
DAPI, dilactate
4,6-diamidino-2-phenylindole, dilactate
DMAO
Nucleus stain (DNA); 2 mM solution in DMSO
EB (ethidium bromide)
Solution
Dead cell stain (DNA), aqueous solution
Ethidium Homodimer I
Nucleus stain (DNA); also known as EthD-1, or EtDi
Ethidium Homodimer III
Nucleus stain (DNA)
Ethidium Homodimer III,

1 mM in DMSO
Nucleus stain (DNA); in DMSO
Hoechst 33258
Nucleus stain (DNA)
Hoechst 33258 Solution
Hoechst 33342
Nucleus stain (DNA)
Propidium Iodide (PI)
Dead cell stain (DNA)
Propidium Iodide (PI) Solution
Dead cell stain (DNA); aqueous solution
1 ml
PK-CA707-40012
10 ml
(10 mg/ml)
PK-CA707-40042
1 mg
PK-CA707-40010
1 mg
PK-CA707-40050
200 l
PK-CA707-40051
100 mg
PK-CA707-40045
10 ml
(10 mg/ml)
PK-CA707-40044
100 mg
PK-CA707-40047
10 ml
(10 mg/ml)
PK-CA707-40046
100 mg
PK-CA707-40016
10 ml
(1 mg/ml)
PK-CA707-40017
Mitochondria Stains
DASPEI
2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide;
for staining of mitochondria in live cells
100 mg
PK-CA707-70018
Dihydrorhodamine 123
Reduced form of Rhodamine 123; mitochondria stain
10 mg
PK-CA707-10055
Dihydrorhodamine 123,
dihydrochloride salt
Reduced form of Rhodamine 123; mitochondria stain
10 mg
PK-CA707-10056
DiOC6(3)
3,3-dihexyloxacarbocyanine, iodide; membrane

potential sensitive dye
100 mg
PK-CA707-70009
Cell Analysis
Applications
110
6 Cell Analysis
Cell Analysis
Product
Product Description
Size
Catalog Number
JC-1 chloride
Stains mitochondria in living cells in a membrane

potential-dependent function
5 mg
PK-CA707-70011
JC-1 iodide
Stains mitochondria in living cells in a membrane

potential-dependent function
5 mg
PK-CA707-70014
Nonyl Acridine Orange

(or NAO)
Mitochondria stain; not dependent on mitochondrial

membrane potential
50 mg
PK-CA707-70012
MitoGreen
Green-fluorescent mitochondrial dye
20 x 50 g
PK-CA707-70054
MitoRed
Red-fluorescent mitochondrial dye
20 x 50 g
PK-CA707-70055
Rhodamine 123
Green-fluorescent mitochondria stain
50 mg
PK-CA707-70010
Tetrabromorhodamine 123,
bromide
Green-fluorescent mitochondria stain
5 mg
PK-CA707-70013
TMRE
Tetramethylrhodamine ethyl ester, perchlorate;

membrane potential sensitive dye
25 mg
PK-CA707-70016
TMRM
Tetramethylrhodamine methyl ester, perchlorate;

25 mg
PK-CA707-70017
100 mg
PK-CA707-70009
ER Stains
DiOC6(3)
3,3-dihexyloxacarbocyanine, iodide;
Viable Cell Stains

BCECF
Viable cell stain (cytosol)
1 mg
PK-CA707-51010
BCECF AM
Viable cell stain (cytosol); acetoxymethyl ester
10 x 100 g
20 x 50 g
1 mg
PK-CA707-51011
PK-CA707-51011-1
PK-CA707-51012
BCECF AM Solution
Viable cell stain (cytosol); acetoxymethyl ester;

DMSO solution
1 mg in 1 ml
PK-CA707-51009
Calcein AM
20 x 50 g
1 mg
PK-CA707-80011-3
PK-CA707-80011
Calcein AM Solution,
1 mg/ml in anhydrous DMSO
Viable cell stain (cytosol); acetoxymethyl ester,

DMSO solution
1 mg in 1 ml
PK-CA707-80011-2
4 mM DMSO solution
Viable cell stain (cytosol); acetoxymethyl ester,

4 mM DMSO solution
100 l
PK-CA707-80011-1
Calcein, high purity
Viable cell stain (cytosol); ultra-pure
100 mg
PK-CA707-80013
CFSE; 5(6)-CFDA, SE
5-(and-6)-carboxyfluorescein diacetate,
succinimidyl ester; viable cell stain (cytosol)
25 mg
PK-CA707-90041
Hoechst 33258
Nucleus stain (DNA)
100 mg
PK-CA707-40045
10 ml
(10 mg/ml)
PK-CA707-40044
Hoechst 33342
Nucleus stain (DNA)
100 mg
PK-CA707-40047
10 ml
(10 mg/ml)
PK-CA707-40046
EB (ethidium bromide)
Solution
10 ml
(10 mg/ml)
PK-CA707-40042
Nucleus stain (DNA); also known as EthD-1, or EtDi
1 mg
PK-CA707-40010
Nucleus stain (DNA)
1 mg
PK-CA707-40050
Ethidium Homodimer III,

1 mM in DMSO
Nucleus stain (DNA); in DMSO
200 l
PK-CA707-40051
Dead cell stain (DNA)
Trypan Blue Solution (0.5%)
Dead cell stain; tetrasodium salt, 0.5% solution
Dead Cell Stains
100 mg
PK-CA707-40016
10 ml (1 mg/ml)
PK-CA707-40017
10 ml
PK-CA902-1209
6 Cell Analysis
111
Product
Product Description
Size
Catalog Number
Blue-fluorescent Cytoplasmic
Membrane Staining Kit
Convenient and sensitive cell staining

with low cytotoxicity
50 assays
PK-CA707-30024
Green-fluorescent Cytoplasmic Membrane Staining Kit

1 ml
PK-CA707-30021
Orange-fluorescent Cytoplasmic Membrane Staining Kit

1 ml
PK-CA707-30022
Red-fluorescent Cytoplasmic
Membrane Staining Kit

1 ml
PK-CA707-30023
Lipophilic Carbocyanine Dyes

DiD (DiIC18[5])
1,1'-dioctadecyl-3,3,3',3'- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt
50 mg
PK-CA707-60014
DiI (or DiIC18[3])
1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine
perchlorate
50 mg
PK-CA707-60010
DiI in vegetable oil
Optimized DiI formulation for microinjection
0.5 ml
PK-CA707-60018
Dilinoleyl DiI
1,1'-dilinoleyl-3,3,3',3'-tetramethylindocarbocyanine
perchlorate (also called Fast DiI, a trademark of
Molecular Probes)
5 mg
PK-CA707-60034
Dilinoleyl DiO
1,1'-dilinoleyl-3,3'-oxacarbocyanine perchlorate
(also called Fast DiO, a trademark of Molecular Probes)
5 mg
PK-CA707-60035
DiO (or DiOC18[3])
3,3'-dioctadecyloxacarbocyanine, perchlorate
50 mg
PK-CA707-60011
DiOC14(3),
hydroxyethanesulfonate
3,3'-ditetradecyloxacarbocyanine,
hydroxyethanesulfonate
50 mg
PK-CA707-60012
DiOC16(3)
Lipophilic cyanine dye for staining cytoplasmic

membranes
25 g
PK-CA707-60038
DiR (DiIC18[7])
1,1'-dioctadecyl-3,3,3',3'-tetramethyl
indotricarbocyanine Iodide
25 mg
PK-CA707-60017
Neuro-DiI
Improved alternative to DiI
25 mg
PK-CA707-60016
Neuro-DiI in vegetable oil
Optimized formulation for microinjection
0.2 ml
PK-CA707-60020
Neuro-DiO
Improved alternative to DiO
25 mg
PK-CA707-60015
Neuro-DiO in vegetable oil
Optimized formulation for microinjection
0.2 ml
PK-CA707-60019
Biotin-DHPE
N-(biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3phosphoethanolamine, triethylammonium salt
10 mg
PK-CA707-60022
Biotin-X-DHPE
N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium
salt
5 mg
PK-CA707-60023
Fluorescein-DHPE
N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, triethylammonium salt
5 mg
PK-CA707-60024
NBD-DHPE
N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt
10 mg
PK-CA707-60025
Rhodamine-DHPE
N-(lissamine rhodamine B sulfonyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium

salt
5 mg
PK-CA707-60026
Texas Red-DHPE
N-(Texas Red sulfonyl)-1,2-dihexadecanoyl-sn-glycero3-phosphoethanolamine, triethylammonium salt
1 mg
PK-CA707-60027
TRITC-DHPE
N-(teramethylrhodamine-6-thiocarbamoyl)-1,2dihexadecanoyl-sn-glycero-3-phosphoethanolamine,
triethylammonium salt
1 mg
PK-CA707-60028
Lipophilic styryl dye with an amine group at the

hydrophilic head
1 mg
PK-CA707-70051
Phospholipid Membrane Probes
Other Membrane Dyes

AM3-25
Cell Analysis
Cytoplasmic Membrane Staining
112
6 Cell Analysis
Cell Analysis
Product
Product Description
Size
Catalog Number
Biotin-AM3-25
AM3-25 conjugate with biotin that can be coupled

to avidin or streptavidin
1 mg
PK-CA707-60032
DiA
4-(4-dihexadecylaminostyryl)-N-methylpyridinium iodide
50 mg
PK-CA707-60013
DiB
Lipophilic blue fluorescent dye useful for staining

cytoplasmic membranes
10 g
PK-CA707-60036
DPA
Nonfluorescent anionic membrane dye that can be used

as a FRET quencher for a variety of fluorescent donor
dyes
25 g
PK-CA707-60037
NBD C6-ceramide
6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)
hexanoyl)sphingosine
1 mg
PK-CA707-60030
NBD C6-sphingomyelin
6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)
hexanoyl)sphingosyl phosphocholine
1 mg
PK-CA707-60031
Octadecyl Rhodamine B
Chloride (R18)
Useful for membrane fusion assays
10 mg
PK-CA707-60033
Nerve Terminal Staining Kits

Nerve Terminal Green
Staining Kit I
Kit contains 5 x 1 mg SynapseGreen C4

and 250 mg ADVASEP-7
1 set
PK-CA707-70030

Staining Kit II
Kit contains 1 mg AM1-43 and 100 mg ADVASEP-7
1 set
PK-CA707-70031

Staining Kit III
Kit contains 1 mg AM1-43 and 100 mg SCAS
1 set
PK-CA707-70031-1

Staining Kit IV
Kit contains 5 x 1 mg SynapseGreen C4

and 100 mg Sulforhodamine 101
1 set
PK-CA707-70032
Nerve Terminal Red

Staining Kit I
Kit contains 5 x 1 mg SynapseRed C2

and 250 mg ADVASEP-7
1 set
PK-CA707-70034
Nerve Terminal Red

Staining Kit II
Kit contains 5 x 1 mg SynapseRed C2

and 100 mg Sulforhodamine 101
1 set
PK-CA707-70035
250 mg
PK-CA707-70029
Nerve Terminal Probes & Related Reagents

ADVASEP-7
(sulfobutyl b-cyclodextrin)
For background fluorescence reduction
Alpha-Bungarotoxin
(from Bungarus multicinctus)
Acetylcholine receptor inhibitor
1 mg
PK-CA707-00010-1
AM1-43
Fixable, activity-dependent fluorescent nerve

terminal probe
1 mg
PK-CA707-70024
AM1-44
Nerve Terminal Probe
1 mg
PK-CA707-70038
AM2-10
1 mg
PK-CA707-70036
AM3-25
1 mg
PK-CA707-70051
AM4-64
1 mg
PK-CA707-70025
AM4-66
1 mg
PK-CA707-70050
Biotin-XX-a-Bungarotoxin
Can be used to isolate and/or image the distribution of

the nicotinic acetylcholine receptor at the neuromuscular
junctions
500 g
PK-CA707-00017
Fluorescein-a-Bungarotoxin
Can be used to image the distribution of the nicotinic

acetylcholine receptor at the neuromuscular junctions
500 g
10 x 50 g
PK-CA707-00011
PK-CA707-00013
SCAS
4-sulfonato calix[8]arene, sodium salt; for background

fluorescence reduction
100 mg
PK-CA707-70037
Sulforhodamine 101-aBungarotoxin
Also known as Texas Red-a-Bungarotoxin
500 g
10 x 50 g
PK-CA707-00015
PK-CA707-00016
SynapseGreen C1
5 mg
5 x 1 mg
PK-CA707-70042
PK-CA707-70043
SynapseGreen C18
(FM3-25)
Nerve Terminal Probe (also known as FM3-25,

a trademark of Molecular Probes, Inc.)
5 mg
5 x 1 mg
PK-CA707-70048
PK-CA707-70049
SynapseGreen C2 (FM2-10)

5 mg
5 x 1 mg
PK-CA707-70044
PK-CA707-70045
6 Cell Analysis
113
Product
Product Description
Size
Catalog Number
SynapseGreen C3
5 mg
5 x 1 mg
PK-CA707-70023
PK-CA707-70026

5 mg
5 x 1 mg
PK-CA707-70020
PK-CA707-70022

5 mg
5 x 1 mg
PK-CA707-70046
PK-CA707-70047
SynapseRed C1
5 mg
5 x 1 mg
PK-CA707-70040
PK-CA707-70041
SynapseRed C2 (FM4-64)
Nerve Terminal Probe (also known as FM4-64, a trademark of Molecular Probes, Inc.)
5 mg
5 x 1 mg
PK-CA707-70021
PK-CA707-70027
SynapseRed C2M (FM5-95)
Nerve Terminal Probe (also known as FM5-95, a trademark of Molecular Probes, Inc.)
5 mg
5 x 1 mg
PK-CA707-70028
PK-CA707-70019
Tetramethylrhodaminea-Bungarotoxin
Can be used to image the distribution of the nicotinic

acetylcholine receptor at the neuromuscular junctions
500 g
10 x 50 g
PK-CA707-00012
PK-CA707-00014
Membrane Potential Sensitive Dyes

DiO/DPA Membrane
Potential Detection Kit
For fluorescent detection of cytoplasmic

membrane potential changes
1 kit
PK-CA707-30037
Di-12-ANEPPQ
Fast-responding membrane potential dye
5 mg
PK-CA707-61015
Di-2-ANEPEQ (JPW1114)
Fast-responding membrane potential dye;

also called JPW1114
5 mg
PK-CA707-61013
Di-4-ANEPPS
5 mg
PK-CA707-61010
Di-8-ANEPPQ
5 mg
PK-CA707-61014
Di-8-ANEPPS
DiBAC4(3)
Bis-(1,3-dibarbituric acid)-trimethine oxanol;

DiIC1(5)
5 mg
PK-CA707-61012
25 mg
PK-CA707-61011
1,1'-dimethyl-3,3,3',3'-tetramethylindodicarbocyanine,
iodide
100 mg
PK-CA707-70015
DiOC2(3)
3,3-diethyloxacarbocyanine, iodide;
100 mg
PK-CA707-70008
DiOC5(3)
3,3-dipentyloxacarbocyanine, iodide;
100 mg
PK-CA707-70007
DiOC6(3)
3,3-dihexyloxacarbocyanine, iodid;
100 mg
PK-CA707-70009
RH237
Fast-responding potentiometric probe
5 mg
PK-CA707-61018
RH414
5 mg
PK-CA707-61016
RH421
25 mg
PK-CA707-61017
RH795
5 mg
PK-CA707-61019
TMRE
Tetramethylrhodamine ethyl ester, perchlorate;

25 mg
PK-CA707-70016
TMRM
Tetramethylrhodamine methyl ester, perchlorate;

25 mg
PK-CA707-70017
Cytosolic Tracers and Related Reagents

Biocytin (e-biotinoylL-lysine)
Can be introduced into cells by microinjection
100 mg
PK-CA707-90055
Biocytin-Hydrazide
Can be introduced into cells by microinjection;

aldehyde-fixable
25 mg
PK-CA707-90060
Biotin-4-Fluorescein
Can be used as polar tracer to study cell morphology
10 mg
PK-CA707-90062
Biotin-Ethylenediamine,
hydrobromide
Equivalent to Neurobiotin; transneuronal tracer
25 mg
PK-CA707-90057
Biotin-Ethylenediamine,
hydrochloride
Functionally equivalent to Neurobiotin;

transneuronal tracer
25 mg
PK-CA707-90075
Biotin-Rhodamine 110
Can be used as polar tracer to study cell morphology
5 mg
PK-CA707-80022
Cell Analysis
114
6 Cell Analysis
Cell Analysis
Product
Product Description
Size
Catalog Number
Calcein AM
1 mg
20 x 50 g
PK-CA707-80011
PK-CA707-80011-3
1 mg/ml in anhydrous DMSO
1 ml
PK-CA707-80011-2
4 mM DMSO solution
100 l
PK-CA707-80011-1
Viable cell stain (cytosol); ultra-pure
100 mg
PK-CA707-80013
DPA/Terbium for
membrane fusion assay
Can be used for vesicle fusion assays
1 set
PK-CA707-80104
DPX
Can be used to study membrane fusion or permeability
500 mg
PK-CA707-80012
Fluorescein-Biotin
5-((N-(5-(N-(6-(biotinoyl)amino)hexanoyl)amino)pentyl)
thioureidyl) fluorescein
5 mg
PK-CA707-80019
Hydroxystilbamidine
Also known as FluoroGoldTM
10 mg
PK-CA707-80014
Hydroxystilbamidine
TM
4% in H2O; also known as FluoroGold
200 l
PK-CA707-80023
Lucifer Yellow Cadaverine
Can be used as fixable fluorescent tracer
25 mg
PK-CA707-80018

Biotin-X, dipotassium salt
Useful tracer for studying neuronal morphology
10 mg
PK-CA707-80017
Lucifer Yellow CH lithium salt
Useful polar tracer for studying neuronal morphology
25 mg
PK-CA707-80015
Lucifer Yellow CH potassium

salt
25 mg
PK-CA707-80016
RuBP-2S
Ruthenium(bis(bathophenanthroline))(bathophenanthrolinedisulfonate
5 mg
PK-CA707-80024
RuBP-4S
Ruthenium(bathophenanthroline)(bis(bathophenanthroli
nedisulfonate)), disodium salt
5 mg
PK-CA707-80025
RuBPS
Ruthenium(tris(bathophenanthrolinedisulfonate),
tetrasodium salt
5 mg
PK-CA707-80021
SDIP/Europium
for membrane fusion assay
Can be used for vesicle fusion assays
1 set
PK-CA707-80105
Sulforhodamine 101

and cell-cell communication
500 mg
PK-CA707-80101
Sulforhodamine B

5g
PK-CA707-80100
Sulforhodamine G

5g
PK-CA707-80102
10 ml
PK-CA707-90082
Related Products
DMSO, anhydrous
Dimethylsulfoxide, anhydrous
Pluronic F-127
Nonionic detergent useful for solubilizing large dye

molecules such as Calcein/AM, Fura-2/AM, Fluo-3/AM,
Indo-1/AM and Rhod-2/AM to facilitate cell loading
1g
PK-CA707-59000
Pluronic F-127,
20% solution in DMSO

1 ml
PK-CA707-59004
Pluronic F-127,
10% in H2O

30 ml
PK-CA707-59005
Note: Please check our website regularly for new cell stains.
6 Cell Analysis
115
6.7 Intracellular Indicators and Related Products
6.7.1 Calcium Indicators

Applications

Intracellular calcium signaling studies

Investigation of signal transduction
and apoptotic cascades
Neuroscience research
Features
High quality and sensitivity
Derivatives for special applications

available
Compatible with standard fluores cence equipment
Calcium ions are important second messengers in cells. They are involved in
muscle contraction, nerve signal transmission, immune cell activation, hormone secretion, etc.
Fluorescent calcium indicators selectively
bind to calcium ions and show a spectral response upon binding Ca2+, i.e.,
fluorescence wavelength or intensity are
altered. This shift of fluorescence wavelength and change of intensity upon calcium chelation can be monitored using
fluorescence microscopy.
PromoKine offers a wide range of fluorescent calcium indicators for detecting
changes in the intracellular Ca2+ concentration, including Fura-2, Fluo-3, Rhod2, and Indo-1 (as cell-impermeable salts
or cell-permeable AM esters) as well as
their low-affinity derivatives Fura-2FF,
n Calcium Indicators
Product
Fluo-3FF, and Indo-1FF which are particularly useful for certain applications
(e.g., spatial and functional characterization of Ca2+ stores or detection of
NMDA-induced neuronal Ca2+ fluxes).
PromoKine also provides a range of nonfluorescent calcium chelators as well as
coelenterazine derivatives which are effective luminescent calcium indicators.
6.7.2 Other Indicators

Applications
pH determination
Iron, zinc, and chloride ion detection
Features
High quality and sensitivity
In addition to our calcium probes, PromoKine also offers pH indicators as well
as indicators for iron, zinc, and chloride
ion detection.
Product Description
Size
Catalog Number
Fluorescent Calcium Indicators

Fluo-3 (pentaammonium salt)
Fluorescent calcium indicator; Ex/Em: 490-506/526
1 mg
PK-CA707-50010
Fluo-3 (pentapotassium salt)
1 mg
PK-CA707-50011
Fluo-3 (pentasodium salt)
1 mg
PK-CA707-50012
Fluo-3 AM
Fluorescent calcium indicator (acetoxymethyl ester)
10 x 100 g
20 x 50 g
1 mg
PK-CA707-50013
PK-CA707-50016
PK-CA707-50014
Fluo-3 AM Solution
Fluorescent calcium indicator (acetoxymethyl ester; 1 mM

in anhydrous DMSO)
1 ml
PK-CA707-50015
Fura-2 (pentaammonium salt)
1 mg
PK-CA707-50030
Fura-2 (pentapotassium salt)
1 mg
PK-CA707-50031
Fura-2 (pentasodium salt)
1 mg
PK-CA707-50032
Fura-2 AM
10 x 100 g
20 x 50 g
1 mg
PK-CA707-50033
PK-CA707-50033-1
PK-CA707-50034
Fura-2 AM Solution
Fluorescent calcium indicator (acetoxymethyl ester; 1 mM

in anhydrous DMSO)
1.0 ml
PK-CA707-50029
Indo-1 (pentaammonium salt)
Fluorescent calcium indicator; Ex/Em: 330/405-485
1 mg
PK-CA707-50040
Indo-1 (pentapotassium salt)
1 mg
PK-CA707-50041
Indo-1 (pentasodium salt)
1 mg
PK-CA707-50042
Indo-1 AM
10 x 100 g
20 x 50 g
1 mg
PK-CA707-50043
PK-CA707-50043-1
PK-CA707-50044
Rhod-2 (triammonium salt)
Fluorescent calcium indicator; Ex/Em: 533-556/~578
1 mg
PK-CA707-50020
Cell Analysis
Signal transduction often starts with the

activation of a receptor on the cell surface which subsequently triggers a cascade of second messengers in the cell.
The change of intracellular ion concentration is a crucial step that helps second
messengers transmit information. Consequently, monitoring and control of intracellular ion concentration is a prerequisite understanding second messengers
and signal transduction in living cells.
116
6 Cell Analysis
Product
Product Description
Size
Catalog Number
Rhod-2 (tripotassium salt)

Rhod-2 (trisodium salt)
1 mg
PK-CA707-50021
1 mg
PK-CA707-50022
Rhod-2 AM
10 x 100 g
1 mg
PK-CA707-50023
PK-CA707-50024
Cell Analysis
Fluorescent High Calcium Indicators

5,5'-dibromo-Bapta
(tetrapotassium salt)
Fluorescent calcium indicator
100 mg
PK-CA707-50004
5,5'-difluoro-Bapta
100 mg
PK-CA707-50006
5,5'-difluoro-Bapta AM
25 mg
PK-CA707-50005
1 mg
PK-CA707-50045
500 g
PK-CA578-F1046
Bis-Fura-2, hexapotassium salt
Fura-2FF (K+ Salt)
Difluorinated derivative of Fura-2 (K+ Salt)
Fura-2FF AM
Difluorinated derivative of Fura-3 (acetoxymethyl ester)
500 g
PK-CA578-F1044
Furaptra, AM ester
Fluorescent calcium indicator (also known as Mag-Fura-2)
10 x 100 g
20 x 50 g
1 mg
PK-CA707-50037
PK-CA707-50039
PK-CA707-50038
Furaptra, tetrapotassium salt
1 mg
PK-CA707-50035
Furaptra, tetrasodium salt
1 mg
PK-CA707-50036
Indo-1FF (K Salt)
Difluorinated derivative of Indo-1 (K Salt)
500 g
PK-CA578-I1086
Indo-1FF AM
Difluorinated derivative of Indo-1 (acetoxymethyl ester)
500 g
PK-CA578-I1084
Fluorescent Near-Membrane Calcium Indicators

FFP-18 AM
Fluorescent near-membrane calcium indicator

(Fura-derivative; acetoxymethyl ester)
1 mg
PK-CA578-F1052-1
FIP-18 AM
Fluorescent near-membrane calcium indicator

(Indo-derivative; acetoxymethyl ester)
500 ug
PK-CA578-F1054
5,5'-dibromo-Bapta
100 mg
PK-CA707-50004
5,5'-dimethyl BAPTA, AM
ester
25 mg
PK-CA707-50007
5,5'-dimethyl BAPTA,
tetrapotassium salt
100 mg
PK-CA707-50008
Bapta (tetracesium salt)
Non-fluorescent calcium chelator
1g
PK-CA707-50001
Bapta (tetrapotassium salt)
1g
PK-CA707-50002
Bapta (tetrasodium salt)
1g
PK-CA707-50003
Bapta AM
Non-fluorescent calcium chelator (acetoxymethyl ester)
25 mg
20 x 1 mg
PK-CA707-50000
PK-CA707-50000-1
Bapta-FF (free acid)
Difluorinated derivative of BAPTA
10 mg
PK-CA578-B1091
Bapta-FF AM
Difluorinated derivative of BAPTA (acetoxymethyl ester)
10 mg
PK-CA578-B1093
Coelenterazine; luminescent calcium indicator

& luciferase substrate
50 g
PK-CA707-10110
Coelenterazine 400a
Coelenterazine; luminescent calcium indicator

& luciferase substrate (also known as DeepBlue C,
a trademark of Perkin Elmer)
50 g
PK-CA707-10125
Coelenterazine cp
Coelenterazine derivative; luminescent calcium indicator

50 g
PK-CA707-10112
Coelenterazine f

50 g
PK-CA707-10114
Coelenterazine fcp

50 g
PK-CA707-10117
Calcium Chelators
Luminescent Calcium Indicators
6 Cell Analysis
117
Product
Product Description
Size
Catalog Number
Coelenterazine h

50 g
PK-CA707-10111
Coelenterazine hcp

50 g
PK-CA707-10113
Coelenterazine i

50 g
PK-CA707-10121
Coelenterazine ip

50 g
PK-CA707-10116
Coelenterazine n

50 g
PK-CA707-10115

50 g
PK-CA707-10122
Coelenterazine and derivatives thereof
1 Kit
PK-CA707-10123
n Other Indicators
Product
Product Description
Size
Catalog Number
Zn Indicators
Zinquin, ethyl ester
Blue-fluorescent Zn indicator
5 mg
PK-CA707-52020
Zinquin, free acid
Blue-fluorescent Zn indicator
5 mg
PK-CA707-52022
100 mg
PK-CA707-52011
Cl Indicators
MQAE
N-(ethoxycarbonylmethyl)-6-methoxyquinolinium
bromide; fluorescent chloride ion indicator
SPQ
6-methoxy-N-(3-sulfopropyl)quinolinium;
fluorescent chloride ion indicator
50 mg
PK-CA707-52010
Tetrakis-(2-pyridylmethyl)ethylenediamine;
heavy metal chelator
100 mg
PK-CA707-59003
5(6)-CFDA
5-(and-6)-carboxyfluorescein diacetate;
fluorescent pH indicator
100 mg
PK-CA707-51014
5(6)-CFDA, SE (CFSE)
5-(and-6)-carboxyfluorescein diacetate,
succinimidyl ester
25 mg
PK-CA707-90041
5-(and-6)-Carboxy-2',7'dichlorofluorescein
Fluorescent pH indicator
200 mg
PK-CA707-51015
5-(and-6)-Carboxy-2',7'dichlorofluorescein diacetate
Fluorescent pH indicator; cell membrane-permeant
100 mg
PK-CA707-51016
5-(and-6)-Carboxy-2',7'dichlorofluorescein diacetate,
succinimidyl ester (CDFDA,
SE)
Fluorescent pH indicator; cell membrane-permeant
25 mg
PK-CA707-90040
5-(and-6)-Carboxyfluorescein
100 mg
PK-CA707-51013
5-Carboxy-2',7'dichlorofluorescein
Fluorescent pH indicator (single isomer)
100 mg
PK-CA707-51017
5-Carboxy-2',7'dichlorosulfonefluorescein
Fluorescent pH indicator (single isomer)
10 mg
PK-CA707-51023
5-CFDA
5-Carboxyfluorescein diacetate (single isomer);

100 mg
PK-CA707-51018
5-FAM
5-Carboxyfluorescein; fluorescent pH indicator
100 mg
PK-CA707-51019
6-CFDA
6-Carboxyfluorescein diacetate (single isomer);

100 mg
PK-CA707-51021
Heavy Metal Ion Indicators

TPEN
pH Indicators
Cell Analysis
118
6 Cell Analysis
Cell Analysis
Product
Product Description
Size
Catalog Number
6-FAM
6-carboxyfluorescein; fluorescent pH indicator
BCECF
2',7'-bis-(carboxyethyl)-5-(and-6)-carboxyfluorescein;
100 mg
PK-CA707-51020
1 mg
PK-CA707-51010
BCECF AM
Fluorescent pH indicator; acetoxymethyl ester
10 x 100 g
20 x 50 g
1 mg
PK-CA707-51011
PK-CA707-51011-1
PK-CA707-51012
BCECF AM Solution
Fluorescent pH indicator; acetoxymethyl ester

(1 mg/ml in dry DMSO)
1 ml
PK-CA707-51009
Flubida-2
5 mg
PK-CA707-51022
n Related Products
Product
Product Description
4-Bromo A-23187 free acid
For in situ calibration of fluorescent calcium indicators
A-23187 free acid
A-23187 Free Acid (calcimycin; or Calcium Ionophore III)
Calcium Calibration Buffer Kit
For calibration of fluorescent calcium indicators
DMSO, anhydrous
Dimethylsufoxide, anhydrous
EDC (EDAC)
1-(3-dimethylaminopropyl-3-ethylcarbodiimide,
hydrochloride)
Flubi-2
Calibration standard for Flubida-2
5 mg
PK-CA707-51024
Ionomycin (calcium salt)
Useful in cell activation experiments when calcium

dose-response data are not required (>90% pure)
5 mg
PK-CA577-1566-5
Ionomycin (free acid)
Useful for studies of Ca2+ transport across biological

membranes and measurement of cytoplasmic free Ca2+;
induces apoptotic neuronal degeneration in embryonic
cortical neurons (>90% pure)
5 mg
PK-CA577-1565-5
Pluronic F-127

molecules such as Fura-2/AM, Fluo-3/AM,
Indo-1/AM, and Rhod-2/AM to facilitate cell loading
1g
PK-CA707-59000
Pluronic F-127, 10% in H2O

30 ml
PK-CA707-59005
Pluronic F-127, 20% solution

in DMSO

1 ml
PK-CA707-59004
Note: Please check our website regularly for new intracellular indicators.
Size
Catalog Number
1 mg
PK-CA707-59006
1 mg
10 mg
PK-CA707-59001
PK-CA577-1501
2 x 50 ml
PK-CA707-59100
10 ml
PK-CA707-90082
100 mg
PK-CA707-59002
6 Cell Analysis
119
6.8 Cell Signaling Assays
Quantitative determination of
cAMP/cGMP levels
Cell signaling and cell growth/
differentiation studies
b-Secretase activity detection
Alzheimers disease drug development
Features
Fast and simple assays
Highly sensitive
cAMP and cGMP Immunoassay Kits

Adenosine 3,5-cyclic monophosphate
(cyclic AMP, cAMP) is one of the most
important second messengers involved
as a modulator in physiological processes. It regulates neuronal, glandular, and
cardiovascular processes as well as immune mechanisms, the nervous system,
cell growth, and cell differentiation. A
number of hormones (e.g. ACTH, TSH,
LH) are known to activate cAMP via the
action of the enzyme adenylate cyclase
which converts ATP to cAMP.
Guanosine 3,5-cyclic monophosphate

(cyclic GMP, cGMP) has been shown to
be present at levels typically 10-100 fold
lower than cAMP in most tissues and is
formed by the action of the enzyme guanylate cyclase on GTP. Some hormones
(e.g., acetylcholine, insulin, and oxytocin) and chemicals (e.g., serotonin and
histamine) cause an increase in cGMP
levels.
The PromoKine cAMP and cGMP competitive immunoassays were developed
for the quantitative determination of
cAMP and cGMP levels. They utilize
polyclonal capture antibodies against
cAMP or cGMP coated on a 96-well
plate and HRP-conjugated cAMP or
cGMP-HRP which competes with the
cAMP or cGMP from the sample. After
HRP-substrate addition, the amount of
cAMP-HRP or cGMP-HRP bound to the
plate can easily be determined spectrophotometrically at 450 nm. The intensity
of the blue color is inversely proportional
to the cAMP or cGMP concentration in
the sample. Due to a novel acetylation
procedure 1-100 fmol cAMP or 0.1-10
pmol cGMP can be detected. This is

approximately 10 times more sensitive
than similar kits.
b-Secretase Fluorometric Assay Kit

b-Secretase (BACE) is a membranebound aspartyl protease that cleaves
the amyloid precursor protein and is
consequently an excellent target for
anti-amyloid therapy in the treatment of Alzheimers disease. Finding
inhibitors of b-secretase is one of the
major goals of Alzheimers disease
drug development. The PromoKine
b-Secretase Fluorometric Assay provides
a convenient, non-radioactive system for
detecting b-secretase activity in biological samples. The kit provides active bsecretase as positive control, b-secretase
inhibitor as negative control, optimized
peptide substrate (conjugated to two
reporter molecules), and buffers for
convenient measurement of b-secretase
activity in mammalian samples.
b-Secretase inhibitors are also available
separately.
Product
Size
Catalog Number
cAMP Immunoassay Kit
100 assays
PK-CA577-K371
cGMP Immunoassay Kit
100 assays
PK-CA577-K372
Beta-Secretase Fluorometric Assay Kit
100 assays
PK-CA577-K360
Beta-Secretase Inhibitor I
1 mg
PK-CA577-7501-1
Beta-Secretase Inhibitor II
1 mg
PK-CA577-7502-1
Cell Analysis
Applications
120
6 Cell Analysis
6.9 Cell Stress and Metabolism Kits
Cell Analysis
Applications
GST activity measurement and quanti-
tation of GST-tagged fusion proteins
SOD assays
Determination of the concentration of
NO metabolites (nitrate/nitrite)
HAT activity measurement
Metabolic studies and analysis of
mutagenic, carcinogenic, and toxic
effects
DNA damage detection and
quantification
Analysis of the cell redox state
CDNB is totally dependent on the presence of active GST. The detection limit
is approximately 10 ng active GST/assay.
The free form of MCB is almost nonfluorescent, whereas the dye fluoresces
blue (Ex/Em = 380/461 nm) when reacting with glutathione. GST catalyzes the
MCB-glutathione reaction and the fluorescence levels are proportional to the
amount of GST present in the reaction.
Therefore, the GST level in samples can
be easily detected using a fluorometer or
a 96-well fluorometric plate reader.
fluorometric reagent DAN followed by

NaOH which converts nitrite into a fluorescent compound (Fluorometric Nitric
Oxide Kits) or the addition of Griess Reagent which converts nitrite into a deep
purple azo compound (Colorimetric
Nitric Oxide Kits). Measurement of the
fluorescent compound reaction product
or azo chromophore accurately determines the total nitric oxide production.
The Fluorometric Assay Kit has been
validated with culture media, plasma,
and tissue homogenates.
SOD Assay Kit
HAT Activity Assay Kit
Superoxide dismutase (SOD) is one of

the most important antioxidative enzymes. The sensitive SOD Assay Kit
utilizes WST-1 that produces a watersoluble formazan dye upon reduction
with the superoxide anion. The rate of
the reduction with a superoxide anion is
linearly related to the xanthine oxidase
(XOD) activity and is inhibited by SOD
(Fig. 8). Therefore, the inhibition activity
of SOD can be determined by a colorimetric method.
Histone acetyltransferases (HATs) have

been implicated in playing a crucial role
in various cellular functions such as gene
transcription, cell differentiation, and
proliferation.
The colorimetric HAT Activity Assay
Kit offers a convenient non-radioactive
system for rapid and sensitive spectrophotometric detection of HAT activity in
mammalian samples. The detection can
be continuous and is suitable for kinetic
studies.
Fluorometric and Colorimetric

Nitric Oxide Kits
DNA Damage Detection Kit
Features
Highly sensitive and reproducible
detection/measurement
Ready-to-use, convenient assays
Fluorometric and Colorimetric GST

Assay Kits
Glutathione (GSH) is the major intracellular low-molecular-weight thiol and plays
a critical role in cellular defense against
oxidative stress in tissues and cells. Glutathione S-transferase (GST) is involved
in detoxification of xenobiotics mediating
conjugation of glutathione with those
compounds.
The Fluorometric and Colorimetric GST
Assays can detect GST activity in crude
cell lysates or purified protein fractions
and the kits also quantitate GST-tagged
fusion proteins. They are based upon the
GST-catalyzed reaction between GSH
and the GST substrate CDNB (Colorimetric Kit) or MCB (Fluorometric Kit).
CDNB has the broadest range of isozyme
detectability (a-, -, f-, and other GST
isoforms). Under certain conditions, the
interaction between glutathione and
Nitric oxide (NO) plays an important role

in neurotransmission, vascular regulation, immune responses, and apoptosis.
The Fluorometric and Colorimetric Nitric
Oxide Assay Kits provide an accurate and
convenient tool for total nitrate/nitrite
concentration determination in a simple
two-step process that can be used as a
quantitative measure of NO production.
The first step is the conversion of nitrate
to nitrite utilizing nitrate reductase. The
second step involves the addition of the
Xanthine
O2
2O2
SO3
O3S
I
N N
H
NO2
Xanthine oxidase
Uric acid
H2O2
WST-1 Formazan
N N
-.
2O2
SO3
O3S
I
N
+
N N
Na +
NO2
SOD
O2 + H2O2
Inhibition reaction
Fig. 8: Principle of the SOD Assay Kit.
WST-1
Different physiological and environmental factors such as chemically induced

damage and cell aging cause DNA lesions. AP sites (apurinic/apyrimidinic
sites) are one of the major types of
DNA lesions formed during the process
of base excision and repair of oxidized,
deaminated, or alkylated bases. It has
been estimated that about 2x105 base
lesions are generated per cell per day.
For this reason, the level of AP sites in
cells can be a good indicator of DNA lesions and repairs. The PromoKine DNA
Damage Detection Kit utilizes the ARP
(Aldehyde Reactive Probe) reagent that
reacts specifically with the aldehyde
group in the open ring form of the AP
sites. After treating DNA containing AP
sites with ARP reagents, the AP sites are
tagged with biotin residues, which can
be quantified using an avidin-biotin assay followed by colorimetric detection.
The kit provides the necessary reagents
for convenient determination of abasic
sites in purified DNA samples in a 96-well
plate format.
NAD/NADH and NADP/NADPH

Quantitation
PromoKines NAD/NADH and NADP/
NADPH Quantitation Kits provide a convenient tool for sensitive detection of the
intracellular nucleotides NAD, NADH,
NADP, and NADPH along with the determination of their ratios. They can be
used for studies of energy transformation and the determination of the redox
state of cells or tissue.
The kits have been designed to specifically recognize NAD/NADH only or NADP/
NADPH only in an enzyme cycling reaction that significantly increases detection
sensitivity. Purification of NAD/NADH or
NADP/NADPH from sample mixes is not
required.
Fluorometric & Colorimetric ATP

Quantitation Kit
ATP that is formed exclusively in the mitochondria is the major energy currency
of living systems and virtually all energy
requiring processes utilize the chemical
energy stored in the phosphate bond
of ATP. A variety of genetic diseases

can affect ATP formation in the mitochondria. There are a number of commercially available ATP assays available
that can detect very low amounts of ATP
by measuring luminescence (PromoKine
Kit PK-CA577-254-200, for example).
However, these kits require specialized
luminescence instrumentation and utilize
luciferase which can be difficult to maintain in an active form.
PromoKines new Colorimetric & Fluorometric ATP Quantitation Kit is designed
to be robust and has a simple straightforward method which utilizes the phosphorylation of glycerol to generate a
product that is easily quantified by colorimetric (max = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The
assay can detect as low as 50 picomole
(1 M) of ATP in various samples.
Fluorometric & Colorimetric ADP

Quantitation Kit
ADP is a product of ATP dephosphorylation and can be rephosphorylated back
to ATP. Dephosphorylation and rephos-
121
phorylation occur via various phosphatases, phosphorylases and kinases. ADP

is stored in platelets and can be released
to interact with a variety of purinergic
receptors. ADP levels regulate several
enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and
chloroplast, although several such processes occur in the cytoplasm. Conventionally, ADP levels have been measured
by luciferase/luciferin mediated assays
after ADP is converted to ATP. However,
the luciferase system is unstable and luminescence equipment is not generally
available in most laboratories.
PromoKines new Fluorometric & Colorimetric ADP Quantitation Kit provides a
convenient colorimetric and fluorometric
method to measure the ADP level. In this
assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be
quantified colorimetrically (max = 570
nm) or fluorometrically (Ex/Em 535/587
nm). The assay is simple, sensitive, stable, and high-throughput adaptable. It
can detect as low as 1 M ADP in biological samples.
Product
Colorimetric GST Assay Kit
Size
Catalog Number
100 assays
PK-CA577-K263
Fluorometric GST Assay Kit
100 assays
PK-CA577-K260
100 assays
PK-CA577-K332
Colorimetric Nitric Oxide Assay Kit
2 x 96 assays
PK-CA577-K262
Fluorometric Nitric Oxide Assay Kit
2 x 96 assays
PK-CA577-K252
SOD Assay Kit

100 assays
PK-CA577-K335
25 assays
PK-CA577-K253
NAD/NADH Quantitation Kit
100 assays
PK-CA577-K337
NADP/NADPH Quantitation Kit
100 assays
PK-CA577-K347
Fluorometric & Colorimetric ATP

Quantitation Kit
100 assays
PK-CA577-K354
Fluorometric & Colorimetric ADP

Quantitation Kit
100 assays
PK-CA577-K355
100 g
PK-RP577-1137-100
1g
PK-CA577-1556-1
HAT protein (P/CAF, active)

GST Inhibitor-2 (ethacrynic acid)
Oxidized Glutathione (GSSG)
1g
PK-CA577-1241-1
Reduced Glutathione (GSSG)
1g
PK-CA577-1242-1
GST protein (active)

Superoxide Dismutase (SOD, human)
1 mg
PK-RP577-1243-1
100 g
PK-RP577-4802-100
Cell Analysis
6 Cell Analysis
122
6 Cell Analysis
6.10 Cell Fractionation and Protein Extraction Kits

Applications
Membrane Protein Extraction Kit
The Membrane Protein Extraction Kit

provides an easy and complete protocol
for isolating integral membrane proteins
efficiently from cultured mammalian
cells. The kit was designed to not only
extract the total cellular membrane proteins but also to specifically purify the
plasma membrane proteins. The procedure offers consistent yield and high
purity (>90%). Membrane proteins prepared using the kit can be utilized in a
variety of applications such as Western
blotting, 2-D gels, enzyme analyses,
etc. The entire procedure takes less than
1 hour.
Cellular protein extraction from

mammalian cells
Integral membrane protein extraction
Preparation of highly enriched cellular
fractions (mitochondria, cytosol,
nucleus) from mammalian cells
Cell Analysis
Features

Convenient and efficient cell fractionation and protein extraction

Easy protocols
Proteins and cell fractions can be used
for many downstream applications
Mammalian Whole Cell Protein

Extraction Kit
The Mammalian Whole Cell Protein
Extraction Kit was optimized for complete extraction of all cellular proteins.
It contains an optimized lysis buffer and
a protease inhibitor cocktail as well as
other reagents for convenient extraction
of mammalian proteins from tissues and
cells under non-denaturing conditions.
The entire procedure takes less than 20
minutes and the proteins can be used for
many downstream applications such as
gel electrophoresis, Western blotting, etc.
Mitochondria/Cytosol
Fractionation Kit
Nuclear/Cytosol Fractionation Kit

The Nuclear/Cytosol Fractionation Kit
provides a complete system that enables the separation of nuclear and
cytoplasmic fractions of mammalian
cells and tissues with little or no crosscontamination, maintaining the nuclear
and cytoplasmic compartments separate
and intact.
The Mitochondria/Cytosol Fractionation

Kit allows isolation of a highly enriched
mitochondrial fraction from the cytosol of mammalian cells in a simple and
staightforward procedure without ultracentrifugation and toxic chemicals.
Cytosol/Particulate Fractionation Kit

The Cytosol/Particulate Fractionation
Kit provides an easy and convenient system for rapid separation of cytosol and
particulate fractions. The separated fractions are compatible with downstream
applications such as assaying cellular
distributions of small molecules and proteins or examining signal transduction
pathways.
Complete Cell Fractionation Kit

The Complete Cell Fractionation Kit is
designed for serial sample preparation of
four distinct protein fractions, including
cytosol, particulate, cytoskeleton, and
nuclear fractions from one sample.
The prepared fractions are suitable for
many downstream assays such as twodimensional gel electrophoresis, protein
immunoblotting, gel-shift assays, enzyme activity assays, reporter assays,
and more.
Product
Mammalian Whole Cell Protein Extraction
Kit
Size
Catalog Number
500 assays
PK-CA577-K269
50 assays
PK-CA577-K268
25 assays
100 assays
PK-CA577-K266-25
PK-CA577-K266-100
Mitochondria/Cytosol Fractionation Kit
25 assays
100 assays
PK-CA577-K256-25
PK-CA577-K256-100
50 assays
PK-CA577-K267
50 assays
PK-CA577-K270
6 Cell Analysis
123
6.11 Protein Size Markers and Protein Gel Stains
FluoProtein Gel Stain is a fast and sensitive fluorescent dye for visualization and
quantitation of proteins separated by
1-D or 2-D SDS-PAGE and an excellent,
economic alternative to common fluorescent gel stains. FluoProtein Gel Stain
normally emits a low fluorescence, but is
strongly fluorescent (bright golden color)
when bound to proteins. The maximum
emission wavelength of protein-bound
FluoProtein Gel Stain is near 570 nm.
FluoProtein Gel Stain gives exceptional
sensitivity and a wide dynamic range for
protein detection.
FluoProtein Gel Stain comes as a 100x
stock solution that is simply diluted with
water by the user to its working concentration. The staining procedure is a simple
two-step protocol (fix and stain) that can
be completed in only 30 minutes. Gels
are fixed with ethanol/acetic acid solution prior to staining with FluoProtein Gel
Stain solution. A destain step is normally
not required. Gels stained with FluoProtein Gel Stain may be directly visualized
with a variety of different UV-based fluorescence imaging systems. The bound
FluoProtein Gel Stain is easily removed
from the protein by immersing the gel
in sufficient water, thus it is compatible
with subsequent enzymatic digestion
and mass spectrometry for proteomic applications.
GelQuick Protein Gel Stain/

Destaining Kit
Traditional staining and destaining
methods for SDS-PAGE protein gels
are known to take several hours and

also leave a high blue background
which reduces the sensitivity of band
detection.
ing a broad range (10-245 kDa) with

ten blue-stained protein bands and
two reference bands at 25 kDa (green)
and 75 kDa (red).
PromoKines GelQuick Gel Stain/Destaining Kit provides a fast and sensitive

method for SDS-PAGE gel staining and
destaining. The procedure only takes 5
minutes for gel staining and 15 minutes
for destaining.
After a total of 20 minutes, visualization
of as little as 5 ng of protein on a waterclear background can be seen.
The kit contains sufficient solutions to
stain and destain up to 40 protein mini
gels.
Cell Analysis
FluoProtein Gel Stain
Prestained RTU Protein Ladders

PromoKines Prestained RTU Protein
Ladders are very cost-effective and
supplied ready-to-use for direct loading onto gels. They can be used for
monitoring protein separation during
SDS-polyacrylamide gel electrophoresis,
estimation of protein sizes, and verification of Western transfer efficiency onto
membranes (PVDF, nylon, or nitrocellulose). They show excellent stability with
sharp bands and allow easy identification as bands of different colors are used.
Each Prestained RTU Protein Ladder is
sufficient for 100-200 mini gels.
The Prestained RTU Protein Ladder I
contains 9 pink-stained protein markers
covering the range from 10 to 175 kDa
and three blue-stained reference bands
at 10, 40 and 90 kDa.
The Prestained RTU Protein Ladder II
is a three-color protein standard cover-
Fig. 9: PromoKines RTU Protein Ladder

I (above) and RTU Protein Ladder II (below) separated on different SDS polyacrylamide gels.
Product
Size
Catalog Number
1 ml
10 ml
PK-MB713-004-1
PK-MB713-004-10
For 40 minigels
PK-MB577-K901
Prestained RTU Protein Ladder I
2 x 0.25 ml
PK-MB713-001P
Prestained RTU Protein Ladder II
0.5 ml
PK-MB713-002P

GelQuick Protein Gel Stain/Destaining Kit
124
6 Cell Analysis
6.12 Nucleic Acid Isolation Kits

Applications
Mitochondrial DNA Isolation Kit
DNA isolation
Fast, simple, and reproducible

procedures
Optimal yields
Highly pure DNA suitable for various
molecular genetic applications
The Mitochondrial DNA Extraction Kit

provides a convenient tool for isolating
mtDNA from a variety of cells and tissues with high yield and purity, without
contamination from genomic DNA. The
purified mtDNA can be used for a variety
of studies such as enzyme manipulation,
Southern blotting, cloning, PCR analysis,
and amplifications.
Genomic DNA Isolation Kit I
PCR Purification Kit
The Genomic DNA Isolation Kit I provides a simple and convenient procedure
for rapid isolation of genomic DNA from
mammalian cells and tissue samples with
high yield and purity. The novel method
requires less than 90 minutes to prepare
highly pure genomic DNA that is free
of protein and RNA and is suitable for
a variety of applications such as PCR,
DNA hybridization, cloning, Southern
blotting, and array-based experiments.
The PromoKine PCR Purification Kit has

been designed for the work-up of PCR
reactions (removal of primer dimers,
primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities). The preparation is based on a
silica-membrane technology for binding
DNA in high-salt and elution in low-salt
buffer. The kit provides a simple and
efficient way to purify linear or circular
DNA and is optimized for working with
Cell Analysis
Features
DNA amounts of up to 50 g. It requires

no organic extractions or precipitation
and guarantees high and stable recovery
rates.
Product
Genomic DNA Isolation Kit I
Size
Catalog Number
50 assays
PK-MB577-K281
50 isolations
PK-CA577-K280
50 preparations
250 preparations
PK-MB20-300-50
PK-MB20-300-250
6 Cell Analysis
125
6.13 DNA Size Markers and DNA Gel Stains

RTU DNA Ladders
PromoKines FluoDNA Gel Stain is a nonmutagenic fluorescent reagent that produces instant visualization of DNA bands
upon Blue Light or UV illumination of
agarose gels.
Supplied in 6x DNA Loading Buffer, it
is used to prepare DNA samples and
markers for loading onto agarose or
polyacrylamide gels. FluoDNA Gel Stain
is a highly sensitive stain available for detecting double-stranded DNA (dsDNA)
and contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for monitoring the DNA migration during the electrophoresis process.
FluoDNA Gel Stain is an economic, safe,
and non-hazardous alternative to ethidum bromide. It provides high sensitivity,
shows a low background, and causes less
DNA damage. It can therefore improve
efficiency in downstream applications
such as gene cloning.
PromoKines RTU DNA Ladders are suitable for use as molecular weight standards for agarose gel electrophoresis.
They are very cost-effective and supplied
ready-to-use, sufficient for 100-200 mini
gels.
The RTU DNA Ladder I displays 11 sharp
bands ranging from 100 to 1,500 base
pairs. Two bands (100 and 1,500 base
pairs) have an increased intensity and
serve as reference points. The ladder
comes ready-to-use containing Orange G
and Xylene Cyanol FF as tracking dyes.
The RTU DNA Ladder II includes 13 fragments ranging from 250 to 10,000 bp
with two bands (1,000 and 3,000 base
pairs) which have an increased intensity to serve as reference points. It comes
ready-to-use containing Bromphenol
Blue as tracking dye.
Base
Pairs
DNA Mass
(ng/5ml)
Base
Pairs
Cell Analysis
DNA Mass
(ng/5ml)
FluoDNA Gel Stain
Fig. 10: PromoKines RTU DNA

Ladder I (left) and RTU DNA Ladder II
(right) separated on 1.7% and 1.0%
TAE agarose gels, respectively.
Product
Size
Catalog Number
RTU DNA Ladder I (100 bp)
0.5 ml
PK-MB713-001N
RTU DNA Ladder II (1000 bp)
0.5 ml
PK-MB713-002N
1 ml
PK-MB713-003
FluoDNA Gel Stain
Apoptosis
Apoptosis, or programmed cell death, is a sophisticated process
that involves an intricate series of biochemical events. Specific
cell death signals are transduced by different biomolecules such
as caspases, which initiate an apoptotic cascade. This process
culminates in multiple events such as degradation of cellular
proteins and chromosomal DNA, mitochondrial disruption, and
changes in plasma membrane integrity.
3
7
Apoptosis
7.1 DNA Fragmentation Detection Kits . . . . . 129
7.2 Mitochondrial Apoptosis Staining Kit . . . . 130
7.3 Cytochrome c Apoptosis Detection Kit . . . 130
7.4 Glutathione Detection Kits . . . . . . . . . . . . 131
7.5 Annexin V Detection Kits . . . . . . . . . . . . . 132
7.6 Caspase Kits . . . . . . . . . . . . . . . . . . . . . . . 134
7.6.1
Caspase Detection Kits . . . . . . . . . . . . . . . . 134
7.6.2
Caspase Quantification Kits . . . . . . . . . . . . 136
7.7 Cathepsin/Calpain Detection Kits . . . . . . . 138

7.8
Kinase Detection Kits . . . . . . . . . . . . . . . . 139
7.9
Apoptotic and Necrotic Cell Detection . . . 140
7.10 Apoptotic Cell Isolation Kit . . . . . . . . . . . . 141

7.11 Apoptosis Inducers . . . . . . . . . . . . . . . . . . 141
7 Apoptosis
129
7.1 DNA Fragmentation Detection Kits
Detecting fragmented
nucleosomal DNA
Screening for apoptotic cells
Fluorescence microscopy
Flow cytometry
Western blotting
Features
High sensitivity
Rapid detection of apoptotic cells at
the single-cell level
Compatible with standard lab
equipment
Internucleosomal DNA fragmentation is
a key sign of apoptosis in mammalian
cells. PromoKine offers four different kits
for analyzing DNA fragmentation which
are based on fluorometric or colorimetric
assays. Since all of the kits are designed
for use with various multiwell formats,
they are also suited for automation.
Fluorometric DNA Fragmentation

Detection Kits I and II (BrdU)
The PromoKine DNA Fragmentation
Detection Kits I and II are based on the
TUNEL method and identify DNA strand
breaks that occur during apoptosis. The
kits provide all required components including positive and negative control
cells and tissue slides - for convenient
detection of DNA fragmentation by fluorescence microscopy or flow cytometry.
The TUNEL assay employs a terminal
deoxynucleotidyl transferase (TdT) to
add Br-dUTP (bromolated deoxyuridine

triphosphate nucleotides) to the fragmented DNA strands. Br-dUTP is more
readily incorporated into DNA strand
breaks than other larger ligands (e.g.,
fluorescein, biotin, or digoxigenin). The
incorporated Br-dUTP is subsequently
labeled with a green- (Kit I) or a redfluorescent (Kit II) anti-BrdU monoclonal
antibody resulting in a very bright signal.
The labeled DNA can then be observed
by fluorescence microscopy or analyzed
by flow cytometry. The DNA Fragmentation Kit II works also very well with GFP
transfected cells.
Fluorometric DNA Fragmentation

Detection Kit III (F-dUTP)
The Fluorometric DNA Fragmentation
Detection Kit III is a modified TUNEL assay. It provides all reagents required for
the specific detection of apoptotic cells in
a cell population, in tissue sections, and
in fixed cell preps. The kit utilizes terminal deoxynucleotidyl transferase (TdT) to
catalyze the incorporation of fluorescein12-dUTP at the free 3-hydroxyl ends of
the fragmented DNA. The fluoresceinlabeled DNA can then be observed by
fluorescence microscopy or analyzed by
flow cytometry.
Colorimetric DNA Fragmentation

Detection Kit (IHC)
The ready-to-use TUNEL-based Colorimetric DNA Fragmentation Detection
Kit (IHC) has been specifically designed
for expedient detection of DNA frag-
mentation in tissue sections and fixed

cell preparations. It includes positive
and negative control slides and utilizes
Br-dUTP, biotin-labeled anti-BrdU antibodies, and HRP-streptavidin conjugate.
Immunohistochemical staining results
can be analyzed with a microscope.
Apoptotic DNA Ladder

Detection Kit I
The Apoptotic DNA Ladder Detection
Kit I provides an easy and sensitive
method for detecting DNA fragmentation in apoptotic cells. DNA preparation
is done in less than 90 minutes without
the need for extraction or separation
columns. Afterwards, the DNA fragments can easily be visualized by agarose gel electrophoresis. This kit allows
the efficient recovery of small DNA fragments ensuring high sensitivity.
The Apoptotic DNA Ladder Detection
Kit IPlus additionally includes a highly
sensitive DNA staining dye for improved
visualization of DNA fragmentation by
agarose gel electrophoresis.
Apoptotic DNA Ladder

Detection Kit II
Using the Apoptotic DNA Ladder Extraction Kit II allows the selective isolation
of DNA ladders without interference of
regular genomic DNA. The kit can detect
apoptotic DNA ladders from tissues and
cells with an apoptotic cell population
of as little as 5%. The kit specifically extracts laddered DNA, not genomic DNA.
Product
Size
Catalog Number
Fluorometric DNA Fragmentation Detection Kit I (BrdU)
60 assays
PK-CA577-K401
Fluorometric DNA Fragmentation Detection Kit II (BrdU)
60 assays
PK-CA577-K404
Fluorometric DNA Fragmentation Detection Kit III (F-dUTP)
50 assays
PK-CA577-K402
Colorimetric DNA Fragmentation Detection Kit (IHC)
50 assays
PK-CA577-K403
Apoptotic DNA Ladder Detection Kit I
50 assays
PK-CA577-K120
50 assays
PK-CA577-K130
Apoptotic DNA Ladder Detection Kit II
50 assays
PK-CA577-K170
Plus
Note: Please check our website regularly for new cell-based apoptosis assays.
Apoptosis
Applications
130
7 Apoptosis
7.2 Mitochondrial Apoptosis Staining Kit

Applications
Screening for early apoptotic cells
Flow cytometry
Apoptosis
Features

Excellent sensitivity due to high

fluorescence intensity
Simultaneous detection of apoptotic
and healthy cells
Disruption of mitochondrial transmembrane potential is one of the earliest

intracellular events that occurs upon the
induction of apoptosis. The Mitochondrial Apoptosis Staining Kit provides a
simple, fluorescent-based in vitro assay
for detecting the mitochondrial changes
in apoptosis, and the kit distinguishes
between healthy and apoptotic cells.
The assay utilizes the novel cationic
dye MitoCaptureTM that fluoresces
differently in healthy versus apoptotic
cells. In healthy cells, MitoCaptureTM
accumulates and aggregates in the mitochondria - fluorescing brightly red. In
apoptotic cells, a shift in mitochondrial

transmembrane potential prevents the
dye from accumulating in the mitochondria. Instead, it remains in the cytoplasm
in its monomer form - fluorescing green.
The fluorescent signals can easily be
detected by fluorescence microscopy or
flow cytometry using common filters
and channels (FITC, rhodamine, PI). The
assay takes approximately 20 minutes, is
highly sensitive, and detects apoptosis
reliably in living cells.
Product
Mitochondrial Apoptosis Staining Kit
Size
Catalog Number
25 assays
100 assays
PK-CA577-K250-25
PK-CA577-K250-100
7.3 Cytochrome c Apoptosis Detection Kit

Applications
Analysis of apoptotic signal cascades
Features
No toxic chemicals necessary
Cytochrome c has been shown to play

an important role in apoptosis. The protein is located between the inner and
outer mitochondrial membranes. An
apoptotic stimulus triggers the release
of cytochrome c into the cytosol where
it binds to Apaf-1. The cytochrome
c/Apaf-1 complex activates caspase-9
which then activates caspase-3 and
other downstream caspases of the apoptotic cascade.
The PromoKine Cytochrome c Apoptosis
Detection Kit provides an effective means
for detecting cytochrome c translocation

from mitochondria into the cytosol during apoptosis. The kit is easy to use and
provides unique reagents for separating
a highly enriched mitochondria fraction
from the cytosol. Cytochrome c released
from mitochondria into the cytosol is
then detected by Western blotting using
the cytochrome c antibody provided in
the kit. The procedure is very simple and
straightforward. No ultracentrifugation
is required and no toxic chemicals are
involved.
Product
Cytochrome c Apoptosis Detection Kit
Size
Catalog Number
100 assays
PK-CA577-K257
7 Apoptosis
131
7.4 Glutathione Detection Kits

Glutathione detection
Quantitation of glutathione
reductase activity
Features
Non-radioactive, highly sensitive
method
equipment
Fluorometric Glutathione
Detection Kit
Diminished glutathione (GSH) levels
occur at the early stages of apoptosis.
The Fluorometric Glutathione Detection
Kit provides a simple in vitro assay for
detecting the GSH changes in apoptosis
and other pathological processes. The
assay utilizes a dye (monochlorobimane,
MCB) that has a high affinity for glutathione. The unbound dye is almost nonfluorescent, whereas the dye fluoresces
blue (excitation=380 nm; emission=461
nm) when bound to reduced or oxidized
glutathione. Consequently, the decrease
in glutathione level in apoptotic cells can
be easily detected using a fluorometer or
a 96-well fluorometric plate reader.
Colorimetric Glutathione
Detection Kit
The Colorimetric Glutathione Detection
Kit provides a convenient colorimetric
method for analyzing either total glutathione or only the reduced form of glutathione using a microplate reader. The
assay utilizes the glutathione recycling
system with DTNB and glutathione reductase. GSH is regenerated from GSSG
(oxidized glutathione) by glutathione
reductase and reacts again with DTNB
to produce yellow 2-nitro-5-thiobenzoic
acid that can be detected spectrophotometrically at 412 nm (Fig. 1). This recycling reaction dramatically improves the
sensitivity of total glutathione detection.
The kit can quantify total glutathione
from 1-100 ng/well. For detecting lower
glutathione concentrations, such as in
DTNB
blood samples, increasing the reaction

time significantly improves the signal.
The kit can also specifically detect the reduced form of glutathione (GSH) when
glutathione reductase is omitted from
the reaction mixture.
Colorimetric Glutathione
Reductase Activity Kit
PromoKines Colorimetric Glutathione
Reductase Activity Kit was designed
for measuring glutathione reductase
(GR) activity in biological samples. It
is highly sensitive and well suited for
high-throughput. The assay is based on
the GR-catalysed reduction of GSSG to
GSH, which reacts with 5,5-Dithiobis
(2-nitrobenzoic acid) (DTNB) to generate the yellow TNB2-. The kit can detect
0.1 40 mU/ml GR in various samples.
2GSH
Glutathione
+ NADPH
reductase
2-Nitro-5-thiobenzoic acid
GSSG
Fig. 1: Principle of the Colorimetric Glutathione Detection Kit.
Product
Size
Catalog Number
Fluorometric Glutathione Detection Kit
100 assays
PK-CA577-K251
Colorimetric Glutathione Detection Kit
100 assays
PK-CA577-K261
Colorimetric Glutathione Reductase

Activity Kit
200 assays
PK-CA577-K761
Apoptosis
Applications
132
7 Apoptosis
7.5 Annexin V Detection Kits

Applications


Differentiating apoptotic and
necrotic cells
Annexin detection
Flow cytometry
Apoptosis
Features
High fluorescence intensity and
sensitivity
Many different labels and tags
available
Fast, convenient protocol suited for
automation and high-troughput
In the early stages of apoptosis, changes
can be observed on the cell surface.
Cells lose their phospholipid membrane
asymmetry and expose phosphatidylserine (PS) on the exterior plasma membrane. Annexin V is a Ca2+-dependent,
phospholipid-binding protein with high
affinity for PS and is very useful for
identifying apoptotic cells that expose
PS on their surfaces to the extracellular
environment. Annexin V may be conjugated to biotin or fluorophores such as
FITC and phycoerythrin (PE). It can also
be tagged with EGFP (Enhanced Green

Fluorescent Protein). Since Annexin V
conjugates and tagged annexins retain
their high affinity for PS, they are ideally suited as highly sensitive probes to
identify early apoptotic cells and to detect apoptosis much earlier than assays
based on DNA fragmentation or loss of
membrane integrity. The stained cells
can be evaluated by flow cytometry or
fluorescence microscopy using common
filter sets and flow cytometer channels.
Annexin V Detection Kits

The Annexin V Apoptosis Detection
Kits include an Annexin V conjugate
or tagged Annexin V as well as binding buffer and in some cases Propidium
Iodide (PI) for differentiating between
apoptotic and dead cells. The kits allow
a one-step staining procedure that takes
only 10 minutes. In addition, the assay
can be performed directly on living cells.
Promokine Annexin V Apoptosis Detection KitPlus includes an additional dye, SYTOX green, that is impermeant to living
and apoptotic cells, but stains necrotic
cells with intense green fluorescence
by binding to cellular nucleic acids. After staining a cell population with e.g.,
fluorescent Annexin V-Cy3 or Annexin
V-PE conjugates, and SYTOX Green dye
in the provided binding buffer, apoptotic

cells show red fluorescence, necrotic cells
show green fluorescence, and live cells
show little or no fluorescence. These
populations can easily be distinguished
by fluorescence microscopy and flow cytometry using the appropriate filters and
channels.
Individual Annexin V Reagents

PromoKine also offers a wide range
of Annexin V reagents separately. For
easy staining of early apoptotic cells,
Annexin V conjugated to FITC, PE, Cy3,
Cy5, or biotin is available, as well as Annexin V tagged with EGFP. The stained
cells can be evaluated by flow cytometry
or fluorescence microscopy using common filter sets and flow cytometer channels. Biotinylated Annexin V can be detected in conjunction with conventional
dye-staining using any streptavidin- or
avidin-dye reagents such as PromoFluorstreptavidin conjugates, fluorescein-, PE-,
or APC-streptavidin as well as streptavidin/avidin conjugated to peroxidase, alkaline phosphatase (AP), b-galactosidase,
etc.
Please visit www.promokine.info to find
our complete range of individual Annexin
V reagents as well as Annexin V antibodies and ELISAs.
Product
Size
Catalog Number
Annexin V-FITC Detection Kit
25 assays
100 assays
400 assays
PK-CA577-K101-25
PK-CA577-K101-100
PK-CA577-K101-400
Annexin V-FITC Detection KitPlus
25 assays
100 assays
400 assays
PK-CA577-K201-25
PK-CA577-K201-100
PK-CA577-K201-400
Annexin V-Cy3 Detection Kit
25 assays
100 assays
400 assays
PK-CA577-K102-25
PK-CA577-K102-100
PK-CA577-K102-400
Annexin V-Cy3 Detection KitPlus
25 assays
100 assays
400 assays
PK-CA577-K202-25
PK-CA577-K202-100
PK-CA577-K202-400
25 assays
100 assays
400 assays
PK-CA577-K103-25
PK-CA577-K103-100
PK-CA577-K103-400
Annexin V-EGFP Detection Kit
25 assays
100 assays
400 assays
PK-CA577-K104-25
PK-CA577-K104-100
PK-CA577-K104-400
7 Apoptosis
133
Product
Size
Catalog Number
Annexin V-PE Detection Kit
25 assays
100 assays
400 assays
PK-CA577-K128-25
PK-CA577-K128-100
PK-CA577-K128-400
Annexin V-PE Detection KitPlus
25 assays
100 assays
400 assays
PK-CA577-K203-25
PK-CA577-K203-100
PK-CA577-K203-400
Annexin V-PE-Cy5 Detection Kit
25 assays
100 assays
400 assays
PK-CA577-K129-25
PK-CA577-K129-100
PK-CA577-K129-400
Annexin V-Biotin Detection Kit
25 assays
100 assays
400 assays
PK-CA577-K109-25
PK-CA577-K109-100
PK-CA577-K109-400
Annexin V-FITC Reagent
200 assays
1000 assays
PK-CA577-1001-200
PK-CA577-1001-1000
Annexin V-Cy3 Reagent
200 assays
1,000 assays
PK-CA577-1002-200
PK-CA577-1002-1000
200 assays
1,000 assays
PK-CA577-1013-200
PK-CA577-1013-1000
Annexin V-EGFP Reagent
200 assays
1,000 assays
PK-CA577-1004-200
PK-CA577-1004-1000
Annexin V-PE Reagent
200 assays
1,000 assays
PK-CA577-1014-200
PK-CA577-1014-1000
Annexin V-PE-Cy5 Reagent
200 assays
1,000 assays
PK-CA577-1015-200
PK-CA577-1015-1000
Annexin V-Biotin Reagent
100 g
1 mg
PK-CA577-1003-100
PK-CA577-1003-1000
Annexin V Unlabeled Reagent
100 g
PK-CA577-1005-100
1X Annexin V Binding Buffer
100 ml
PK-CA577-1035
10X Annexin V Binding Buffer
100 ml
PK-CA577-1006
Apoptosis
134
7 Apoptosis
Apoptosis
7.6 Caspase Kits

Caspases, a family of cysteine aspartic
acid proteases, are important factors
in the progression of apoptosis and
can be activated by various harmful
physiological stimuli such as cytochrome c
release due to mitochondrial membrane
disruption, DNA damage, ER stress,
or inducers such as TNF-alpha and
FasL. Caspases are normally present in
the cell as inactive pro-enzymes. During apoptosis, they are activated by
proteolysis forming a proteolytic cascade that results in the cleavage of distinct proteins that are essential for cell
viability.
7.6.1 Caspase Detection Kits
Caspase Staining Kits
Applications
The fluorometric Caspase Staining Kits

are the ideal tools for detecting activated
caspases in situ in living cells. The kits
utilize potent caspase inhibitors conjugated to FITC or rhodamine as fluorescent markers. The caspase inhibitors
are cell-permeable, nontoxic, and bind
specifically and irreversibly to the activated caspases in apoptotic cells. With
the FITC or rhodamine labels, activated
caspases in apoptotic cells can be directly
visualized by fluorescence microscopy or
analyzed by using flow cytometry, or a
fluorescence plate reader. Caspase inhibitors without FITC or rhodamine labels
are also included in the kits as negative
controls.
The components of the kits (e.g. the
Wash Buffer) are also available separately (please see www.promokine.info).

Flow cytometry and fluorescence
microscopy
Caspase detection
Features
High fluorescent intensity and
sensitivity
Highly specific
Fast and simple
Product
Size
Catalog Number
Green Multi-Caspase Staining Kit
25 assays
100 assays
PK-CA577-K180-25
PK-CA577-K180-100
Green Caspase-2 Staining Kit
25 assays
100 assays
PK-CA577-K182-25
PK-CA577-K182-100
25 assays
100 assays
PK-CA577-K183-25
PK-CA577-K183-100
25 assays
100 assays
PK-CA577-K188-25
PK-CA577-K188-100
25 assays
100 assays
PK-CA577-K189-25
PK-CA577-K189-100
25 assays
100 assays
PK-CA577-K172-25
PK-CA577-K172-100
Red Multi-Caspase Staining Kit
25 assays
100 assays
PK-CA577-K190-25
PK-CA577-K190-100
Red Caspase-3 Staining Kit
25 assays
100 assays
PK-CA577-K193-25
PK-CA577-K193-100
25 assays
100 assays
PK-CA577-K198-25
PK-CA577-K198-100
25 assays
100 assays
PK-CA577-K199-25
PK-CA577-K199-100
Note: Please check our website regularly for new cell-based apoptosis assays. For a complete list of active recombinant caspases,
caspase substrates, inhibitors, and antibodies please visit our website www.promokine.info, or see the PromoKine Price List.
7 Apoptosis
Caspase Drug Screening Kits
The PromoKine Caspase Screening Kit

(FC) provides a convenient means for
detecting the activation of caspases by
flow cytometry in living cells. The assay
is based on the cleavage of (aspartyl)2Rhodamine 110 (D2R), a reported substrate for members of caspase family
proteases. The caspase substrate D2R is
nonfluorescent. However, upon cleavage of the substrate by cellular activated
caspases, the released rhodamine 110
gives rise to fluorescence that can be
measured by flow cytometry (excitation
maximum at 488 nm, emission maximum at 530 nm).
Inhibition of caspases has been shown

to delay apoptosis, implicating a potential role in drug screening efforts.
PromoKine provides a full line of Caspase Drug Screening Kits for effective
screening of caspase inhibitors using
fluorometric methods. The assays utilize
peptide substrates containing consensus
cleavage sequences specific for each
caspase, labeled with AFC (7-amino4-trifluoromethyl coumarin). Active
caspases (provided in the kits) cleave
the synthetic substrates to release free
AFC which can then be quantified using

a fluorescence plate reader. Compounds
to be screened can be directly added to
the reaction, and the level of caspase
inhibition can then be determined. The
assays are simple, straightforward, and
can be performed directly in microtiter
plates. Assay conditions for each caspase
have been optimized to obtain the maximal activity.
Apoptosis
Caspase Screening Kit (FC)
135
Product
Caspase Screening Kit (FC)
Size
Catalog Number
25 assays
100 assays
PK-CA577-K200-25
PK-CA577-K200-100
Caspase-1 Drug Screening Kit
100 assays
PK-CA577-K151-100
100 assays
PK-CA577-K152-100
100 assays
PK-CA577-K153-100
100 assays
PK-CA577-K154-100
100 assays
PK-CA577-K155-100
100 assays
PK-CA577-K156-100
100 assays
PK-CA577-K157-100
100 assays
PK-CA577-K158-100
100 assays
PK-CA577-K159-100
100 assays
PK-CA577-K160-100
caspase substrates, inhibitors, and antibodies please visit our website www.promokine.info, or see the PromoKine Price List.
136
7 Apoptosis
7.6.2 Caspase Quantification Kits

Applications
Apoptosis screening
Quantification of caspase activity
Apoptosis
Features
Highly sensitive and specific
Fast and convenient
equipment
Suited for automation
Caspase Fluorometric &

Colorimetric Assay Kits
With the Caspase Fluorometric & Colorimetric Assay Kits caspase activity in mammalian cells can be quantified by either
fluorescence or absorbance in a fast and
simple procedure. The kits utilize the caspase substrate (Ac-DEVD)2-R110 that is
fluorogenic as well as chromogenic. It
has an extinction coefficient that is 10
times higher than that of pNA - making
the assay extremely sensitive.
Caspase Fluorometric Assay Kits

The Caspase Fluorometric Assay Kits
are intended for convenient and sensitive quantification of caspase activity
in cell lysates. The kits utilize synthetic
peptides which mimic the optimal substrate for distinct caspases and are labeled with 7-amino-4-trifluoromethyl
coumarin (AFC). Activated caspases in
apoptotic cells specifically cleave their
synthetic substrates, resulting in the release of free AFC which is then quantified using a fluorometer. The kits are
fast, straight-forward, and adaptable to
96-well plates.
Caspase Colorimetric Assay Kits

The Caspase Colorimetric Assay Kits are
intended for convenient and sensitive
quantification of caspase activity in cell
lysates. The kits utilize synthetic peptides
which mimic the optimal substrate for
distinct caspases and are labeled with
p-nitroaniline (pNA). Activated caspases
in apoptotic cells specifically cleave their
synthetic substrates, resulting in the release of free pNA, which is then quantified using a spectrophotometer. The kits
are fast, straightforward, and adaptable
to 96-well plates.
Caspase Fluorometric Assay

Kits (HTS)
The Caspase Fluorometric Assay Kits
(HTS) were designed for economic highthroughput applications. They use the
fluorogenic and chromogenic substrate
(Ac-DEVD)2-R110 and include specific
caspase inhibitors as negative controls.
Caspase-3 and Caspase-7

Immunoassay Kits
Caspase-3 plays a key role in the
initiation of cellular events during the
apoptotic process. The PromoKine
Caspase-3 and Caspase-7 Immunoassay
Kits allow quantification of the specific
activity of caspase-3 and -7. Since caspase-3 and -7 share the same target
substrate sequence (DEVD), it is difficult
to differentiate the cleavage activity contributed by these two caspases in vitro.
Consequently, the assays utilize caspase-3 or -7 specific antibodies to capture the activated caspase-3 or -7 in cell
lysate. The specific activity of caspase-3
or -7 can then be analyzed using the
common caspase-3/7 substrate DEVDAFC. The assay system ensures specific
detection of caspase-3 or caspase-7 ac-
tivity in apoptotic samples. Other caspases and non-specific proteases are not
detected.
Individual Caspase Reagents

In addition to the complete caspase
staining and activity kits, PromoKine also
provides a variety of high quality, purified active recombinant caspases and
pro-caspases from human, mouse and
rat, as well as caspase substrates and
inhibitors. Optimized assay buffers and
other supplementary reagents for setting
up custom assays are also available separately. These reagents allow researchers
to perform numerous caspase assays
economically. Sets of ready-to-use caspase enzymes, caspase substrates, and
caspase inhibitors provide more convenience in setting up individual assays to
evaluate multiple caspases in the same
experiment.
PromoKines active caspases are very
useful for studying enzyme regulation
and kinetics, screening caspase inhibitors, determining target substrates, and
as positive controls in caspase activity
assays and Western blot analyses.
The caspase substrates come in readyto-use form for both fluorometric and
colorimetric caspase assays.
Please visit www.promokine.info or
see the PromoKine Price List to find the
complete range of PromoKine individual
caspase reagents.
A range of caspase antibodies and
ELISAs can also be found on our website.
7 Apoptosis
137
Product
Size
Catalog Number
Caspase-1 Fluorometric Assay Kit
25, 100, 200, 400 assays
PK-CA577-K110-XXX*
25, 100, 200, 400 assays
PK-CA577-K116-XXX*
25, 100, 200, 400 assays
PK-CA577-K105-XXX*
25, 100, 200 assays
PK-CA577-K126-XXX*
25, 100, 200, 400 assays
PK-CA577-K122-XXX*
25, 100, 200, 400 assays
PK-CA577-K114-XXX*
25, 100, 200, 400 assays
PK-CA577-K112-XXX*
25, 100, 200, 400 assays
PK-CA577-K118-XXX*
25, 100 assays
PK-CA577-K124-XXX*

25, 100 assays
PK-CA577-K139-XXX*
Caspase-1 Colorimetric Assay Kit
25, 100, 200, 400 assays
PK-CA577-K111-XXX*
25, 100, 200, 400 assays
PK-CA577-K117-XXX*
25, 100, 200, 400 assays
PK-CA577-K106-XXX*
25, 100, 200 assays
PK-CA577-K127-XXX*
25, 100, 200, 400 assays
PK-CA577-K123-XXX*
25, 100, 200, 400 assays
PK-CA577-K115-XXX*
25, 100, 200, 400 assays
PK-CA577-K113-XXX*
25, 100, 200, 400 assays
PK-CA577-K119-XXX*
25, 100 assays
PK-CA577-K125-XXX*
25 assays
100 assays
PK-CA707-30011-1
PK-CA707-30011-2
1 ml
10 ml
100 ml
PK-CA707-30012-1
PK-CA707-30012-2
PK-CA707-30012-3
Caspase-3 Immunoassay Kit
100 assays
PK-CA577-K163
100 assays
PK-CA577-K167

Caspase-8 Fluorometric & Colorimetric Assay Kit
Caspase-8 Fluorometric Assay Kit (HTS)
caspase substrates, inhibitors, and antibodies please visit our website www.promokine.info or see the PromoKine Price List.
* XXX stands for the respective package size: 25, 100, 200 or 400 tests.
Apoptosis
138
7 Apoptosis
7.7 Cathepsin/Calpain Detection Kits

Applications
Apoptosis
Features

Compatible with standard lab equipment
and various multi-well formats
Apoptosis can be mediated by mechanisms other than the well-studied caspase-mediated cleavage cascade. There
is growing knowledge that alternative
proteolytic enzymes such as the lysosomal cathepsin proteases may initiate
or propagate proapoptotic signals, but
it is currently unclear how cathepsin
mediates apoptosis.
Cathepsin Activity Assay and

Cathepsin Inhibitor Screening Kits
The Cathepsin Activity Assay Kits are
based on fluorometric or colorimetric
(Cathepsin G assay) detection and utilize the preferred substrate sequence
for each cathepsin, labeled with AFC
(amino-4-trifluoromethylcoumarin), MCA
(7-amino-4-methyl coumarin) or pNA
(p-nitroaniline). Cell lysates and other
samples that contain cathepsins cleave
the synthetic substrate to release free
AFC, MCA or pNA that can be easily
quantified using a fluorometer/photometer or a fluorescence plate reader/
ELISA reader. The cathepsin assays are
easy to perform and can be adapted to
96-well plate assays. Assay conditons
have been optimized to obtain the maximal activity.
The fluorometric Cathepsin D Inhibitor
Screening Kit includes active Cathepsin
D enzyme and a synthetic Cathepsin
D substrate coupled to MCA. It is a
simple, straightforward assay that can
be adapted to 96-well plate assays and
high-throughput screening. The relative
efficacy of test inhibitors are compared
to the positive control inhibitor, Pepstatin A.
A range of Cathepsin antibodies and
ELISAs can also be found on our website.
processes (e.g., apoptosis). The Calpain

Activity Assay Kit provides a simple and
convenient means for analysing calpain
activity in apoptotic cells and other
samples. The assay is based on fluorometric detection and quantitation of the
cleavage of a calpain substrate at 505
nm using a fluorometer or a fluorescence
plate reader. The kit contains positive
and negative controls. Comparison of
the fluorescence intensity from a treated
sample with a normal control allows
determination of the changes in calpain
activity.
The fluorometric Calpain-1 Inhibitor
Screening Kit includes active Calpain-1
enzyme and a synthetic Calpain-1
substrate. It provides a rapid, simple,
sensitive, and reliable test suitable for
high-throughput screening of Calpain-1
inhibitors. In the presence of potent Calpain-1 inhibitors, the hydrolyzation of
the calpain substrate will be impeded.
An inhibitor control is included to compare the efficacy of test inhibitors.
Calpain Activity Assay and Calpain

Inhibitor Screening Kits
A range of different Calpain antibodies

can also be found on our website.
The activation of calpain is involved in

many physiological and pathological
Product
Cathepsin B Activity Assay Kit
Size
Catalog Number
100 assays
PK-CA577-K140
Cathepsin D Activity Assay Kit
100 assays
PK-CA577-K143
Cathepsin D Inhibitor Screening Kit
100 assays
PK-CA577-K148
Cathepsin G Activity Assay Kit
100 assays
PK-CA577-K146
Cathepsin H Activity Assay Kit
100 assays
PK-CA577-K145
Cathepsin K Activity Assay Kit
100 assays
PK-CA577-K141
Cathepsin L Activity Assay Kit
100 assays
PK-CA577-K142
Cathepsin S Activity Assay Kit
100 assays
PK-CA577-K144
Calpain Activity Assay Kit
100 assays
PK-CA577-K240
Calpain-1 Inhibitor Screening Kit
100 assays
PK-CA577-K244
Cathepsin B, human (native)
5 g
PK-RP577-1021
Cathepsin D, human (native)
5 g
PK-RP577-1022
Cathepsin H, human (native)
5 g
PK-RP577-1023
5 g
100 g
PK-RP577-1135-5
PK-RP577-1135-100
5 g
PK-RP577-1025
Cathepsin L, human, recombinant

Cathepsin S, human (native)
Calpain I, human (native)
Cathepsin B & L Substrate Z-FR-R110
Cathepsin Substrate Z-FR-AMC
100 g
PK-RP577-1134
5 mg
PK-CA707-10209
25 mg
PK-CA707-10201
Calpain Inhibitor Z-LLY-FMK
20 l (10 mM)
PK-CA577-1125-20C
Cathepsin B&L Inhibitor Z-Phe-Phe-FMK
20 l (10 mM)
PK-CA577-1126-20C
7 Apoptosis
139
7.8 Kinase Detection Kits

Kinase detection and activity
screening
Features
Easy, accurate, and fast procedure
equipment
large numbers of samples. The assay

utilizes an N-terminal c-Jun fusion protein bound to Glutathione-Sepharose
beads to selectively pull down JNK
from cell lysate. After a washing step to
remove non-specifically bound proteins,
the kinase reaction is carried out in the
presence of cold ATP. c-Jun phosphorylation is then determined by Western blot
analysis using a phospho-c-Jun specific
antibody.
JNK Activity Detection Kit

The activation of protein kinases, such
as JNK and Akt, plays an important role
in apoptosis and other physiological and
pathological processes. PromoKine provides a novel non-radioactive system for
convenient measurement of JNK and
Akt activities.
JNK Activity Screening Kit

The JNK Activity Screening Kit can
screen JNK activity quickly even with
The JNK Activity Detection Kit utilizes

a JNK-specific antibody to immunoprecipitate JNK from cell lysates. The
activity of the JNK is then detected in a
kinase reaction using recombinant c-Jun
as substrate. c-Jun phosphorylation is
determined by Western blotting using
a phospho-c-Jun specific antibody. The
kit specifically detects JNK activity; other
kinase activities are not detected.
Akt Activity Detection Kit

The Akt Activity Detection Kit utilizes
an Akt-specific antibody to immunoprecipitate Akt from cell lysates. Akt-specific
activity is then detected in a kinase reaction using recombinant GSK-3a as
a substrate. Phosphorylation levels of
GSK-3a are determined by Western blot
analysis using a phospho-GSK-3a specific antibody included in the kit. The kit
specifically detects Akt1, Akt2, and Akt3
activities. Other kinase activities are not
detected.
Active JNK and Akt kinases as well
as JNK/Akt substrates, activators, inhibitors, and control cell lysates are also
available separately.
Please visit www.promokine.info or see
the PromoKine Price List to find more
active kinases that can be used for
cell analysis and apoptosis research. A
range of kinase antibodies can also be
found on our website.
Product
Size
Catalog Number
40 assays
PK-CA577-K430
40 assays
PK-CA577-K431
40 assays
PK-CA577-K435
1 mg
PK-CA577-1701
Akt Inhibitor
Chk2 Inhibitor
1 mg
PK-CA577-1702
c-Jun (1-79)-GST Fusion Protein
100 ug
PK-CA577-7001
GSK-3a-GST Fusion Protein
100 ug
PK-CA577-7003
GSK-3, human, recombinant
100 ug
PK-CA577-7004
L-JNK Peptide Inhibitor I
50 l (1 mM)
PK-CA577-7011
L-JNKI1 Negative Control Peptide
50 l (1 mM)
PK-CA577-7021
JNK Negative Control Cell Lysate
1 mg
PK-CA577-7031
JNK Activated Cell Lysate
1 mg
PK-CA577-7032
Akt Negative Control Cell Lysate
1 mg
PK-CA577-7035
Akt Activated Cell Lysate
1 mg
PK-CA577-7036
Note: Please check our website regularly for new cell-based apoptosis assays. For a complete list of active recombinant kinases,
kinase substrates, inhibitors, and antibodies please visit our website www.promokine.info or see the PromoKine Price List.
Apoptosis
Applications
140
7 Apoptosis
7.9 Apoptotic and Necrotic Cell Detection

Applications

Simultaneous detection of apoptotic,

necrotic, and healthy cells
Flow cytometry
Apoptosis
Features
Convenient ready-to-use kit
Fast procedure
Highly sensitive
Apoptosis and necrosis, the two major

processes leading to cell death, show
several distinct differences. One characteristic of apoptotic cells is the translocation of phosphatidylserine from the
inner to the outer side of the plasma
membrane. This allows phagocytic cells
to recognize and destroy apoptotic
cells but can also be used for identifying apoptotic cells. Necrotic cells, on
the other hand, are characterized by a
loss of membrane integrity. This allows
membrane-impermeable probes to enter
and stain them.
Apoptotic/Necrotic Cells
Detection Kit
The Apoptotic/Necrotic Cells Detection
Kit provides convenient quantification of
apoptotic (green) and necrotic (red) cells
within the same cell population by flow

cytometry or fluorescence microscopy.
For tagging apoptotic cells, fluorescein
labeled Annexin V is used. Annexin V
specifically binds to the phosphatidylserine exposed on the outer membrane of
apoptotic cells. In order to mark necrotic
cells, Ethidium homodimer III (EthD-III)
is utilized. This fluorescent DNA binding substance can enter necrotic cells
because of the damaged plasma membrane, but not living or apoptotic cells.
Once in the necrotic cell, it binds to cellular DNA and fluoresces red. Due to its
significantly higher fluorescence quantum yield, Ethidium homodimer III is
superior to propidium iodide or Ethidium
homodimer I.
Fig. 2: Apoptotic U937 cells stained

with Annexin V-FITC using PromoKines
Apoptotic/Necrotic/Healthy Cells Detection Kit.
Apoptotic/Necrotic/Healthy Cells
Detection Kit
The Apoptotic/Necrotic/Healthy Cells
Detection Kit provides convenient quantification of apoptotic (green), necrotic
(red), and healthy cells (blue) within the
same cell population by flow cytometry
or fluorescence microscopy. In addition
to the fluorescent dyes utilized in the
Apoptotic/Necrotic Cells Detection Kit,
it also contains a membrane-permeable
blue fluorescent DNA stain (Hoechst
33342) for quantifying healthy cells.
Fig. 3: Apoptotic/Necrotic U937 cell

stained with Ethidium homodimer III
(EthD-III) using PromoKines Apoptotic/
Necrotic/Healthy Cells Detection Kit.
Product
Size
Catalog Number
Apoptotic/Necrotic Cells Detection Kit
50 assays
PK-CA707-30017
Apoptotic/Necrotic/Healthy Cells
Detection Kit
50 assays
PK-CA707-30018
7 Apoptosis
141
Applications
Isolation of apoptotic cells
Removal of dead cells from culture
Features
Fast, simple, and efficient
Complete ready-to-use kit
The PromoKine Apoptotic Cell Isolation
Kit provides a simple and efficient means
for isolating apoptotic cells or removing
MagBeads adhere to the magnet while normal cells stay in suspension.

The bound apoptotic cells can then be
released from the MagBeads using the
Elution Buffer provided in the kit. The
separated apoptotic cells and normal
cells can be used in a variety of assays to
study apoptotic mechanisms and pathways. The kit has also been successfully
used to remove dead cells from normal
cultures.
A re-usable magnetic separator optimized for the kit is also available.
dead cells from cell culture or tissue

preparations using Annexin V magnetic
beads (MagBeads). Since Annexin V is a
phospholipid-binding protein with high
affinity for phosphatidylserine (PS), it
binds strongly to the PS that is redistributed from the inner to the outer surface
of the plasma membrane during apoptosis. Annexin V-biotin first binds to apoptotic cells. Then, biotin binds to streptavidin-MagBeads, which enables the
separation of apoptotic cells from normal
cells. The apoptotic cells bound to the
Product
Apoptotic Cell Isolation Kit
Size
Catalog Number
30 isolations
PK-CA577-K258
PK-CA577-K1999-1
Magnetic Separator (re-usable)
7.11 Apoptosis Inducers

Applications
Induction of apoptotic processes
Ideally suited for use with diverse
apoptosis detection systems
Features
High quality
Ready-to-use
PromoKine offers a select choice of high

quality apoptosis inducers in ready-touse form. Some of them are also available
in a convenient set. The apoptosis inducers are well-characterized and effective
in disrupting mitochondrial transmembrane potential and activating caspases
as well as inducing phosphatidyl-serine
(PS) exposure, DNA fragmentation, and
other apoptotic characteristics. All inducers are ideally suited for use with the
various PromoKine apoptosis detection

systems.
PromoKine provides a wide range of
high quality growth factors, cytokines,
ELISAs, and antibodies that are relevant
to apoptosis research (see our website).
Product
Size
Catalog Number
50 l
PK-CA577-1036-50
Actinomycin D
5 mg
50 mg
PK-CA577-1036-5MG
PK-CA577-1036-50MG
Anisomycin
10 mg
PK-CA577-1549-10
1 kit
PK-CA577-K121-5
25 mg
PK-CA577-1552-25
5 mg
PK-CA577-1560-5
50 mg
500 mg
PK-CA577-1039-50MG
PK-CA577-1039-500MG
Actinomycin D (10 mM)
Apoptosis Inducer Set

Betulinic acid
Brefeldin A
Camptothecin
Apoptosis
7.10 Apoptotic Cell Isolation Kit
142
7 Apoptosis
Product
Camptothecin (2 mM)
Carboplatin
Catalog Number
1 ml
PK-CA577-1039-1
100 mg
PK-CA577-1553-100
Colchicine
1g
PK-CA577-1519
Cycloheximide
1g
PK-CA577-1041-1G
Cycloheximide (100 mM)
1 ml
PK-CA577-1041-1
10 mg
50 mg
PK-CA577-1524-10
PK-CA577-1524-50
Dexamethasone
1g
10 g
PK-CA577-1042-1G
PK-CA577-1042-10G
Dexamethasone (10 mM)
1 ml
PK-CA577-1042-1
Daunorubicin.HCl
Apoptosis
Size
Doxorubicin.HCl
Etoposide
Etoposide (10 mM)
Genistein
5 mg
PK-CA577-1527-5
25 mg
250 mg
PK-CA577-1043-25MG
PK-CA577-1043-250MG
100 l
PK-CA577-1043-100
10 mg
100 mg
PK-CA577-1533-10
PK-CA577-1533-100
5 mg
PK-CA577-1566-5
5 mg
PK-CA577-1565-5
Mifepristone
50 mg
PK-CA577-1561-50
Mitomycin C
5 mg
10 mg
100 mg
PK-CA578-M1108-5
PK-CA578-M1108-10
PK-CA578-M1108-100
Okadaic acid
25 g
PK-CA577-1543-25
Phorbol-12-myristate 13-acetate (PMA)
5 mg
PK-CA577-1544-5
5 mg
50 mg
PK-CA577-1568-5
PK-CA577-1568-50
Rosiglitazone
5 mg
100 mg
PK-CA577-1559-5
PK-CA577-1559-100
Staurosporine
100 g
1 mg
PK-CA577-1048-010
PK-CA577-1048-1
1g
PK-CA577-1551
Rapamycin
Tamoxifen citrate
Thapsigargin
1 mg
PK-CA577-1558-1
Tyrphostin AG 1295
5 mg
PK-CA577-1571-5
Tyrphostin AG 490
5 mg
PK-CA577-1570-5
Note: Please check our website regularly for new apoptosis inducers.
The availability of new fluorophores has dramatically changed
the possibilities for the sensitive detection of biomolecules and
the analysis of their interactions. Improved fluorescent dyes are
now enabling previously impossible studies of cellular structures and processes. PromoKine provides a wide range of highquality fluorophores for easy labeling of diverse biomolecules
including proteins, antibodies, and DNA, as well as complete
labeling kits and ready-to-use fluorescent conjugates.
3
8
8.1
PromoFluor Dyes . . . . . . . . . . . . . . . . . . . 145
8.2
PromoFluor Labeling Kits . . . . . . . . . . . . . 148
8.2.1
PromoFluor Biomolecules Labeling Kits . . . . 148
8.2.2
PromoFluor DNA Labeling Kits . . . . . . . . . . 149
8.3
PromoFluor Labeled Biomolecules . . . . . . 150
8.3.1
PromoFluor Labeled Antibodies . . . . . . . . . 150
8.3.2
PromoFluor Labeled Phalloidin . . . . . . . . . . 150
8.3.3
PromoFluor Labeled Nucleotides . . . . . . . . . 151
8.3.4
PromoFluor Labeled Biotin and Streptavidin 152
8.4
Phycobiliprotein Dyes . . . . . . . . . . . . . . . . 152
8.5
PromoFluor Antifade Reagent . . . . . . . . . 153
8 Fluorescent Labeling
145
8.1 PromoFluor Dyes
Labeling biomolecules such as

proteins, antibodies, and nucleic acids
Flow cytometry
Fluorescent in situ hybridization
(FISH)
Fluorescence correlation spectroscopy
(FCS)
DNA and protein microarrays
Features

Strong light absorption and high

fluorescence intensity
Very photostable
Highly fluorescent over a broad pH
range (pH 4 - 9)
Water-soluble without the need to
use organic solvents
PromoFluor dyes are powerful fluorophores spanning almost the whole visible
and infrared light spectrum (see Table 1).
They are superior to many other commonly used fluorescent dyes and well
qualified for a wide range of cell and

molecular biology applications. In addition to the standard dyes, specifically
designed PromoFluor-LSS dyes offer an
extra large Stokes shift, which makes
them ideally suited for multicolor labeling and flow cytometry applications.
All PromoFluor dyes are provided
either as free carboxylic acids or in
three reactive variants ready for covalent coupling to biomolecules:
amine-reactive NHS-Esters, AminoModified Labels, and thiol-reactive
Maleimides.
PromoFluor NHS-Esters
PromoFluor NHS-Esters (N-hydroxysuccinimidyl-esters) are intended for covalently labeling biomolecules containing a
primary amino group (all peptides/proteins contain at least one amino group
at the N-terminus). Moreover, amino
groups can also be incorporated in other
biomolecules without changing their
activity or functionality. The most common examples are 5-amino-modified
DNA oligomers and cDNA containing
aminoallyl-dU.
PromoFluor Amino-Modified Labels

PromoFluor Amino-Modified Labels are
intended for the covalent labeling of biomolecules containing a carboxy group
e.g., glycoproteins. The amino group
of PromoFluor Amino-Modified Labels
can be cross-coupled to carboxy groups
through a mild in situ activation.
PromoFluor Maleimides
PromoFluor Maleimides are intended for
covalently labeling biomolecules containing a thiol group (e.g. proteins). A
neutral thiol-reactive label has little influence on the isoelectric point of a protein.
In effect, PromoFluor Maleimides have
an advantage over amino group labels
because they do not interfere with techniques like differential 2D gel electrophoresis. Since most proteins contain
only a few thiol functional groups, thiolreactive dyes can be used to selectively
modify a protein or peptide containing
a thiol group at a specific site. Moreover,
these dyes are well suited for labeling
oligonucleotides. Maleimides couple to
thiol groups under very mild conditions
at a pH around 7.
Fig. 1: Left: Cell structures of HeLa cells visualized using primary anti-beta-tubulin antibodies and secondary PromoFluor 488-labeled antibodies (green) as well as primary anti-centromere antibodies and secondary PromoFluor 547-labeled antibodies (red).
Middle: Rat cytokeratin (green) detected with primary rabbit anti-cytokeratin antibodies and secondary anti-rabbit IgGs labeled
with PromoFluor 488. Actin (red) visualized using mouse anti-actin antibodies and secondary goat anti-mouse IgGs labeled with
PromoFluor 520LSS. Right: Solid-state fluorescence of PromoFluor 633.
Fluorescent
Labeling
Applications
146
Fluorescent
Labeling
Name
Absorption
Maximum
[nm]
Emission
Maximum
[nm]
Description
Laser Excitation Source*
PromoFluor 350
354
431
Alternative to Alexa Fluor 350,

AMCA, AMCA-X
PromoFluor 405
400
420
Alternative to Alexa Fluor 405, Pacific Blue,

Cascade Blue
Blue diode laser
PromoFluor 415
418
467
Alternative to Pacific Blue, Spectrum Aqua,

and Diethylaminocoumarin
Blue diode laser
PromoFluor 488LSS
485
560
Dye with extra large Stokes shift.

Ideally suited for multicolor labeling
and flow cytometry applications
Argon 488 laser
PromoFluor 488
493
521
Alternative to Fluorescein (FITC), Alexa

Fluor 488 and CyTM2
Argon 488 laser
PromoFluor 488
Premium
490
516
Very photostable alternative to Fluorescein

(FITC), Alexa Fluor 488, CyTM2
Argon 488 laser
PromoFluor 500LSS
500
630

Argon 488 laser
PromoFluor 505
505
530
Alternative to Rhodamine GreenTM
Argon 488 laser
PromoFluor 510LSS
509
590

Argon 514 laser
PromoFluor 514LSS
515
650

Argon 514 laser
PromoFluor 520LSS
520
664

Argon 514 laser
PromoFluor 532
539
561

Tetramethylrhodamine (TMR and TRITC)
Nd:YAG laser
PromoFluor 546
551
572

Tetramethylrhodamine (TMR and TRITC)
Helium-Neon 543 laser
PromoFluor 555
557
574
Alternative to Alexa Fluor 555, CyTM3,

and Tetramethylrhodamine (TMR)
PromoFluor 590
581
598
Alternative to Texas Red
PromoFluor 610
610
630
Alternative to Texas Red
PromoFluor 633
637
657
Alternative to Alexa Fluor 633

and 635 diode laser
PromoFluor 647
653
672

CyTM5, and Allophycocyanin
Krypton-Argon 647
laser
PromoFluor 670
673
694
Alternative to Alexa Fluor 680 and CyTM5.5
Helium-Neon laser
Argon laser or
UV-light
PromoFluor 680
690
709
Alternative to Alexa Fluor 680, Cy 5.5
Helium-Neon laser
PromoFluor 700
704
723
Alternative to Alexa Fluor 700
Far-red diode lasers
PromoFluor 750
748
772
Alternative to Alexa Fluor 750, CyTM7
Far-red diode lasers
PromoFluor 770
769
796
Alternative to Alexa Fluor 750 and CyTM7
Xenon-arc lamp or
dye-pumped laser
PromoFluor 780
783
800
Near infrared dye allowing for deep tissue

penetration and low background
Xenon-arc lamp or
dye-pumped laser
PromoFluor 840
844
Near infrared dye allowing for deep tissue

penetration and low background
Xenon-arc lamp or
dye-pumped laser
TM
Table 1: List of currently available PromoFluor dyes (for new PromoFluor dyes that may be added in the future please visit our website).
*PromoFluor dyes are compatible with common excitation sources including diode lasers, LEDs, tungsten lamps, xenon arc lamps, and custom made systems.
147
Excitation / Emission [%]

100
80
60
Fig. 2: Excitation (left) and

emission (right) spectra of
PromoFluor 488LSS.
Fluorescent
Labeling
40
20
300
350
400
450
500
550
600
650
700
Wavelength [nm]
PromoFluor ChipPacks
PromoFluor ChipPacks are pre-packed
PromoFluor dyes intended to label
cDNA for microarray assays. The packs

contain 20 g aliquots of PromoFluor
555 and PromoFluor 647 NHS-Ester.
These aliquots are sufficient for labeling
the amount of cDNA synthesized in a

standard reverse transcription assay with
enzymatic incorporation of aminoallyldUTP.
Product
Size
Catalog Number*
PromoFluor Carboxylic Acid
1 mg
5 mg
PK-PFXXX-0-01
PK-PFXXX-0-05
PromoFluor NHS-Ester
1 mg
5 mg
PK-PFXXX-1-01
PK-PFXXX-1-05
PromoFluor Amino-Modified Label
1 mg
5 mg
PK-PFXXX-2-01
PK-PFXXX-2-05
0.5 mg
1 mg
5 mg
PF-PFXXX-3-005
PK-PFXXX-3-01
PK-PFXXX-3-05
20 labeling reactions
PK-PF-CP-S
PK-PF-CP-M
PK-PF-CP-L
PromoFluor Maleimide
PromoFluor ChipPack
Coupling protocols for proteins are available on our website: www.promokine.info

Note: Different standard sizes are available for PF-488 and PF-505 carboxylic acids and NHS esters.
* XXX- stands fo the respective PromoFluor dye (e.g. PF-350, PF-405, PF-415 etc., see Table 1). Please see our website for an up-to-date list of available
PromoFluor Dyes.
148
8.2 PromoFluor Labeling Kits
Fluorescent
Labeling
8.2.1 PromoFluor Biomolecules Labeling Kits

Applications
Features
Labeling biomolecules such as

proteins, antibodies, and nucleic acids
Flow cytometry
(FISH)
(FCS)

Convenient ready-to-use kits

Contain superior PromoFluor dyes
(see page 146)
Highly efficient labeling
Very bright and stable fluorescent
signals
PromoFluor Biomolecules Labeling Kits

were developed for the convenient and
highly efficient labeling of target proteins or antibodies with PromoKines
superior PromoFluor dyes. They contain
the respective PromoFluor NHS-Ester
as well as all the other components required for labeling biomolecules.
PromoFluor Biomolecules Labeling Kits
work with all molecules with a primary
amino group such as peptides, proteins,
5-aminomodified DNA oligomers, and
cDNA containing aminoallyl-dU.
Fig. 3: Cell structures labeled with

antibodies bound to green-fluorescing
PromoFluor 505 (left) and red-fluorescing PromoFluor 647 (red) dyes.
Illustrations kindly provided by Covalys
Biosciences AG, Switzerland.
Product
PromoFluor-XXX-Labeling-Kit
Size
Catalog Number*
5 reactions
10 reactions
PK-PF-LK-XXX-05
PK-PF-LK-XXX-10
*XXX- stands fo the respective PromoFluor dye (PF-405, PF-415, PF-488P, PF-488LSS, PF-514LSS, PF-546, PF-555, PF-633, PF-647, PF-670, PF-750,
PF-770; please see our website for an up-to-date list of available PromoFluor Labeling Kits).
149
Applications

DNA labeling by PCR

DNA labeling by nick translation
Labeling of probes for FISH, micro-
arrays, Southern hybridizations, etc.
Features
Convenient and fast DNA labeling
Optimal incorporation of labeled
dUTPs
Highly fluorescent and stable fluoro-
phores

PromoKines PCR Labeling Kits and Nick
Translation Labeling Kits were designed
for convenient direct DNA labeling by
PCR or nick translation using Taq polymerase or DNA PolymeraseI/DNaseI.
The fluorescently labeled dUTP analogs provided with the kits have been
optimized for enzymatic incorporation
into DNA by using a proprietary linker
technology. The high incorporation rates
of the dye-dUTP analogs combined with

the outstanding stability and quantum
yield of the fluorophores make the kits
the ideal choice for all typical DNA labeling applications such as FISH, single
molecule detection, microarray gene
expression profiling, and other nucleic
acid hybridization assays. PromoKines
PCR Labeling Kits contain all reagents
required for highly efficient, rapid, and
easy-to-perform DNA labeling (excluding primers and template DNA). The
Nick Translation Labeling Kits contain all
reagents required for efficient, rapid and
easy-to-perform DNA labeling by nick
translation (excluding template DNA).
The labeled dUTPs are also available
separately (see page 151).
In addition, we also provide a kit for nonfluorescent labeling of DNA with biotin.
The biotinylated DNA can then interact
with streptavidin or strepatavidin conjugates (see page 152) for detection or
purification applications.
Fluorescent
Labeling
8.2.2 PromoFluor DNA Labeling Kits
Fig 4: PromoKines DNA Labeling Kits

have been used to generate fluorescently labeled DNA probes for staining
specific chromosome regions.
Product
Product Description
Size
Catalog Number
PromoFluor-430 PCR Labeling Kit
Blue-green fluorescent DNA labeling

by PCR
10 reactions
50 reactions
PK-PF430-PCRLK-10
PK-PF430-PCRLK-50
Green-fluorescent DNA labeling

by PCR
10 reactions
50 reactions
PK-PF500-PCRLK-10
PK-PF500-PCRLK-50
Yellow-fluorescent DNA labeling

by PCR
10 reactions
50 reactions
PK-PF550-PCRLK-10
PK-PF550-PCRLK-50
Orange-fluorescent DNA labeling

by PCR
10 reactions
50 reactions
PK-PF594-PCRLK-10
PK-PF594-PCRLK-50
Red-fluorescent DNA labeling

by PCR
10 reactions
50 reactions
PK-PF640-PCRLK-10
PK-PF640-PCRLK-50
TexasRed PCR Labeling Kit

by PCR
10 reactions
50 reactions
PK-PFTXR-PCRLK-10
PK-PFTXR-PCRLK-50
Biotin PCR Labeling Kit
Kit for biotin-labeling of DNA by PCR
100 reactions
500 reactions
PK-CA-BIO-LK100
PK-CA-BIO-LK500
PromoFluor-430 Nick Translation

Labeling Kit
Blue-green fluorescent DNA labeling

by nick translation
10 reactions
50 reactions
PK-PF430-NTLK-10
PK-PF430-NTLK-50

Labeling Kit
Green-fluorescent DNA labeling

by nick translation
10 reactions
50 reactions
PK-PF500-NTLK-10
PK-PF500-NTLK-50

Labeling Kit
Yellow-fluorescent DNA labeling

by nick translation
10 reactions
50 reactions
PK-PF550-NTLK-10
PK-PF550-NTLK-50

Labeling Kit

by nick translation
10 reactions
50 reactions
PK-PF594-NTLK-10
PK-PF594-NTLK-50

Labeling Kit
Red-fluorescent DNA labeling

by nick translation
10 reactions
50 reactions
PK-PF640-NTLK-10
PK-PF640-NTLK-50
Note: Please see our website for an up-to-date list of available PromoFluor DNA Labeling Kits.
150
8.3 PromoFluor Labeled Biomolecules

8.3.1 PromoFluor Labeled Antibodies
Applications
Fluorescent
Labeling
Flow cytometry
(FISH)
(FCS)
Features

Ready-to-use antibody conjugates of

all PromoFluor dyes (see page 146) are
.
available as goat anti-human IgGs, goat
anti-rabbit IgGs, and goat anti-mouse
IgGs. The labeled secondary antibodies
(polyclonal) are affinity-purified and
dissolved in 100 mM PBS (pH 7.5) at a
concentration of 1 mg/ml. Each antibody
comes with a batch-specific absorption
spectrum.
Fig 5: Tubulin and centromere structures of HeLa cells visualized by
using specific primary antibodies and
secondary antibodies labeled with
PromoFluor 488 and PromoFluor 555.
High quality and ready-to-use

Labeled with PromoFluor dyes
High intensity yields and stable
fluorescent signals
Product
Size
Catalog Number*
PromoFluor-XXX-Goat Anti-Mouse IgG
1 mg
PK-PFXXX-AK-M1
PromoFluor-XXX-Goat Anti-Rabbit IgG
1mg
PK-PFXXX-AK-R1
PromoFluor-XXX-Goat Anti-Human IgG
1 mg
PK-PFXXX-AK-H1
*XXX- stands for the respective PromoFluor dye (PF405, PF488P, PF488LSS, PF500LSS, PF505 etc). Please see page 146 for details.
8.3.2 PromoFluor Labeled Phalloidin

Applications
Localization and visualization of actin
filaments
Features


Labeled with the superior PromoFluor
dyes (see page 146)
Yields highly intensive and stable
fluorescent signals
Phalloidin binds very selectively to actin

(much more tightly to actin filaments
than to actin monomers). In combination with conjugated PromoFluor dyes,
phalloidin is enormously useful in localizing actin filaments in living or fixed cells
as well as for visualizing individual actin
filaments in vitro. Ready-to-use phalloidin conjugates of all PromoFluor dyes
are available (10 nmol, 300 units).
Fig. 6: Phalloidin and centromere
structures of HeLa cells stained using a
PromoFluor 647-Phalloidin conjugate
and primary anti-centromer antibodies
in combination with secondary antibodies labeled with PromoFluor 488.
Product
PromoFluor-XXX, phalloidin conjugate
Size*
Catalog Number**
10 nmol
PK-PFXXX-7-01
*10 nmol are sufficient for staining approximately 300 microscopic slides.
**XXX- stands for the respective PromoFluor dye (PF405, PF488P, PF488LSS, PF500LSS, PF505 etc). Please see page 146 for details.
151
Applications
Features
properties with regard to signal intensity,

water solubility, and stability. In addition,
the low molecular weight of the dyes
results in minimal steric hindrance and
unproblematic incorporation into DNA
using conventional enzymatic techniques like PCR/RT-PCR. AminoallyldUTP can be incorporated into DNA by
Taq polymerase, Klenow fragment, and
reverse transcriptases. All PromoFluor labeled nucleotides are delivered as 1 mM
ready-to-use aqueous solutions.
Fluorescent labeling of nucleic acids

(FISH)
Multicolor FISH (chromosome
painting)
Microarrays

Labeled with superior PromoFluor
dyes (see page 146)
fluorescent signals
Minimal steric hindrance
PromoKine provides aminoallyl-dUTP
(deoxyuridine-triphosphate) conjugates
for all PromoFluor dyes (see page 146).
The labeled nucleotides offer excellent
Fig 7: 2-color labeling of chromosomes

1 and 8 using dUTPs conjugated to
PromoFluor 505 and PromoFluor 555.
Picture kindly provided by Applied
Spectral Imaging.
Fig 8: Fluorescence laser scan of a microarray slide using PromoFluor dyes. Different
matching oligonucleotides, negative controls, and reference oligonucleotides were
spotted in 25 replicates and at 4 different concentrations to epoxysilane coated slides
and hybridized with PromoFluor labeled samples. Picture kindly provided by the
Functional Genomics Center Zurich, Switzerland.
Product
PromoFluor-XXX-aadUTP
Size
Catalog Number*
100 l
PK-PFXXX-8-100
PromoFluor-430-dUTP (NT)
10 l
50 l
PK-PF430-DUTP-10N
PK-PF430-DUTP-50N
PromoFluor-430-dUTP (PCR)
10 l
50 l
PK-PF430-DUTP-10P
PK-PF430-DUTP-50P
10 l
50 l
PK-PF500-DUTP-10N
PK-PF500-DUTP-50N
10 l
50 l
PK-PF500-DUTP-10P
PK-PF500-DUTP-50P
10 l
50 l
PK-PF550-DUTP-10N
PK-PF550-DUTP-50N
10 l
50 l
PK-PF550-DUTP-10P
PK-PF550-DUTP-50P
10 l
50 l
PK-PF594-DUTP-10N
PK-PF594-DUTP-50N
10 l
50 l
PK-PF594-DUTP-10P
PK-PF594-DUTP-50P
10 l
50 l
PK-PF640-DUTP-10N
PK-PF640-DUTP-50N
10 l
50 l
PK-PF640-DUTP-10P
PK-PF640-DUTP-50P
TexasRed-dUTP (PCR)
10 l
50 l
PK-PFTXR-DUTP-10P
PK-PFTXR-DUTP-50P
Biotin-dATP (PCR)
10 l (1 mM)
50 l (1 mM)
PK-CA-BIO-DATP-10P
PK-CA-BIO-DATP-50P
Biotin-dCTP (PCR)
200 l (1 mM)
1 ml (1 mM)
PK-CA-BIO-DCTP-200P
PK-CA-BIO-DCTP-1000P
Biotin-dUTP (PCR)
200 l (1 mM)
1 ml (1 mM)
PK-CA-BIO-DUTP-200P
PK-CA-BIO-DUTP-1000P
* XXX- stands for the respective PromoFluor dye (PF-405, PF-488P, PF-488LSS, PF-500LSS, PF-505 etc). Please see page 146 for details.
Fluorescent
Labeling
8.3.3 PromoFluor Labeled Nucleotides
152
8.3.4 PromoFluor Labeled Biotin and Streptavidin

Applications
Flow cytometry
Fluorescent in situ hybridization (FISH)
(FCS)
Fluorescent
Labeling
Features


Labeled with superior PromoFluor
dyes (see page 146)
fluorescent signals
Streptavidin has a very strong affinity

for biotin with a dissociation constant
of approximately 10-15 mol/l. This is
the highest known affinity between any

protein and its ligand. Extensive chemical
modifications have little effect on this affinity. Streptavidin is a tetrameric 53 kDa
protein. Since it lacks any carbohydrate
modification and has a near-neutral pH,
it has the advantage of much lower nonspecific binding than avidin. On the other hand, streptavidin is less watersoluble
than avidin.
Ready-to-use streptavidin and biotin
conjugates of all PromoFluor dyes (see
page 146) are available. The streptavidin conjugates are affinity-purified and
dissolved in 100 mM PBS (pH 7.5) at a
concentration of 1 mg/ml. The biotin
conjugates are affinity-purified and lyophilized. Each conjugate comes with a
batch-specific absorption spectrum.
Fig. 9: Staining of plasma membranebound neurokinin-1 receptor (red) in a

U2OS cell using a PromoFluor 547-conjugate. Picture kindly provided by Covalys Biosciences AG, Switzerland.
Product
Size
PromoFluor-XXX, streptavidin conjugate

PromoFluor-XXX, biotin conjugate
Catalog Number*
1 mg in 1 ml PBS
PK-PFXXX-5-01
1 mg
PK-PFXXX-6-01
Note: Please see our website www.promokine.info for an up-to-date list of available fluorescent labeled biomolecules.
*XXX- stands for the respective PromoFluor dye (PF-405, PF-488P, PF-488LSS, PF-500LSS, PF-505 etc). Please see page 146 for details.
8.4 Phycobiliprotein Dyes

Phycobiliproteins are water-soluble
proteins with covalently bound chromophores present in cyanobacteria
and certain algae that capture light energy during photosynthesis. The major
phycobiliproteins are R-Phycoerythrin
(R-PE), B-Phycoerythrin (B-PE), and Allophycocyanin (APC).
as large Stokes shifts. The fluorescence

yield is equivalent to at least 30 unquenched fluorescein or 100 rhodamine
molecules at comparable wavelengths
(on a molar basis), and fluorescence is
not quenched by external agents since it
is protected by covalent binding to the
protein backbone.
Phycobiliproteins can be used as fluorescent dyes to label biomolecules to obtain

specific probes or markers for highly sensitive cell and molecular biological applications such as immunofluorescence
microscopy, flow cytometry, microarrays,
FISH, enzyme kinetic studies, etc. They
show optimal quantum yields (up to
98%) and extinction coefficients as well
PromoKines phycobiliproteins can easily

be linked to antibodies. The sensitivity
of antibodies conjugated with R-PE and
B-PE is usually five to ten times greater
than that of the corresponding fluorescein conjugate.
PromoKine offers R-PE, B-PE, and APC
for labeling of biomolecules such as pro-
teins, antibodies, and nucleic acids. They

show strong light absorption, high fluorescence yields, excellent stability and
high fluorescence over a broad pH range
and are compatible with common excitation sources and filter sets.
Please see www.promokine.info/products/fluorescent-labeling for more information and pricing.
153
Applications
Mounting medium for fluorescence
microscopy
Features

Significantly increased photostability

Compatible with many common dyes
Conserves cell preparations
Convenient packaging
Irreversible photobleaching processes

lead to a loss of fluorescent signal sensitivity particularly when high intensity excitation light is used for a longer duration
and the target molecules in samples are
in low abundance. PromoFluor Antifade
Reagent significantly increases the photostability of fluorochromes in fixed cells,
fixed tissues, and cell-free preparations.
It is compatible with many common
dyes and dye complexes resulting in prolonged signals and improved image con-
trast especially in multicolor applications.

Moreover, it gently solidifies after the attachment of the cover slide, permanently
conserving the cell preparation and fixing the coverslip. PromoFluor Anitfade
Reagent makes slide immobilization using e.g. nail polish unnecessary. Samples
can be saved for months after mounting,
retaining fluorescence during prolonged
storage.
PromoFluor Antifade Reagent comes
premixed in a convenient dropper bottle.
Fig. 10: Immunofluorescence imaging

of a human keratinocyte stained with
FITC-labeled antibodies without (top)
and with (bottom) PromoFluor Antifade
Reagent at different excitation intervals
(5, 10, 15, 20, 25 seconds).
Product
Product Description
Size
Catalog Number
PromoFluor Antifade Reagent
Mounting Medium for Fluorescence

Microscopy
8 ml
PK-PF-AFR1
Fluorescent
Labeling
8.5 PromoFluor Antifade Reagent
Cell Transfection
The ability to introduce foreign nucleic acids into eukaryotic
cells has become a powerful tool in studying and controlling
gene expression. Cloned genes can be transfected into cells for
biochemical characterization, mutational analyses, investigating
gene regulatory elements, and producing specific proteins. Cell
transfection promotes the rapid advancement of our knowledge
of genetic regulation and protein function.
3
9
Cell Transfection
9.1 PromoFectin Transfection Reagents . . . . . 157
9.2 Magnet Assisted Transfection (MATra) . . . 161
9.3 Transfection Accessories . . . . . . . . . . . . . . 165
9.3.1 GeneBooster . . . . . . . . . . . . . . . . . . . . . . . . 165
9.3.2
9.4
Cell Clean . . . . . . . . . . . . . . . . . . . . . . . . . . 165
Reporter, Expression and Fusion Vectors . . 166
9.4.1
pPK-CMV Reporter Vectors . . . . . . . . . . . . 166
9.4.2
pPK-CMV Expression Vectors . . . . . . . . . . . 166
9.4.3
pPK-CMV Fusion Vectors . . . . . . . . . . . . . . 166
9 Cell Transfection
157
9.1 PromoFectin Transfection Reagents

PromoFectin
PromoFectin is a series of reagents for

highly efficient and reproducible transfection of a variety of adherent and
non-adherent cells that can be used
to transfect nucleic acids or proteins.
PromoFectin comes either as a broadrange reagent or as specialized reagents
for specific cell types and biomolecules.
Efficient transfection of many cell

types including primary cells
Suited for plasmids, oligonucleotides,
siRNA, and polypeptides
For stable and transient transfections
Features
Superior transfection efficiency
Fast procedure and easy handling
without medium change
Compatible with serum and
antibiotics
Good cell survival and viability after
transfection
The standard broad-range PromoFectin

is a non-liposomal transfection reagent
based on a water-soluble, cationic polymer. It is free of animal-derived components and shows very low cytotoxicity.
PromoFectin ensures highly effective
delivery of plasmids, oligonucleotides,
and siRNA into a variety of adherent
Cytoplasm
Nucleus
Nucleic acid
PromoFectin
Cationic
complexes
Endocytosis
Endosome
Proton
sponge
Nucleic acid
in 150 mM NaCl
solution
Incubate
30 minutes at RT
PromoFectin
Incubate 24-28 h.
Then, measure
protein expression.
and non-adherent cell types with a high

degree of reproducibility. Since its development, PromoFectin has been used
successfully with many problematic cell
lines and sensitive primary cells.
Cell Transfection
Applications
Fig. 1: Primary fibroblasts (NHDF, see

page 27) transfected with the GFP
reporter vector pPK-CMV-R4 (see page
166) using PromoFectin.
Fig. 2: PromoFectin assembles nucleic

acids into compact structures that bind
to the cell surface and are delivered
into the cell by endocytosis. In the
endosome, PromoFectin acts as a proton sponge, buffering the endosomal
pH that leads to pH inhibition of endosomal nucleases. Then, continuous
proton influx leads to osmotic swelling
and rupture of the endosome - allowing for almost complete release of the
nucleic acids into the cytosol. Moreover, PromoFectin also favours DNA
trafficking to the cell nucleus a property that is particularly useful for transfecting slow-dividing or resting cells.
Fig. 3: The PromoFectin transfection procedure is fast and easy. Add

PromoFectin to the nucleic acid solution, mix, incubate for 15-30 minutes, add growth medium (which can
contain serum and antibiotics), and
pipette the complexes onto the cells.
The cells are then incubated for further
analysis. No post-transfection removal
of complexes or medium change/addition is necessary.
158
9 Cell Transfection
Cell Transfection
Fig. 4: Comparison of transfection efficiencies for PromoFectin

and three other common transfection reagents recommended
for primary cells. Primary human fibroblasts (NHDF, see page
27) cultured in 24-well plates were transfected with a luciferase reporter plasmid*. The appropriate protocols recommended by the manufacturers were used for transfection
with PromoFectin and the competing reagents. All transfections were performed in triplicate (values normalized to
1 mg protein).
* See page 166 for reporter plasmids and page 107 for reporter assays.
PromoFectin-HUVEC
PromoFectin-Hepatocyte
PromoFectin-HUVEC is a non-liposomal
PromoFectin derivative optimized for
the transfection of primary endothelial
cells such as HUVEC1 and HDMEC2. It
shows very little cytotoxicity and enables
very high transfection rates also with
fragile, sensitive cell types. Once inside
the cell, PromoFectins unique proton
sponge property provides an excellent
escape mechanism for DNA particles to
the cytoplasm.
PromoFectin-Hepatocyte is a ligandconjugated, non-liposomal derivative of

PromoFectin specialized for hepatocyte
transfection (e.g., primary human and
murine hepatocytes, hepatocyte cell
lines, and hepatocarcinoma cells). The
reagent/nucleic acid complexes bind
specifically to surface receptors on the
target cells leading to extremely high
transfection rates.
PromoFectin-Macrophage was developed for optimal transfection of the

known hard-to-transfect macrophages
(e.g. primary human macrophages
obtained from blood monocytes) and
macrophage-derived cell lines such as
RAW 264.7. PromoFectin-Macrophage
is a mannose-bearing variant of the
standard PromoFectin reagent. It binds
to cells expressing mannose-specific
membrane receptors - such as macrophages, monocytes and dendritic cells leading to enhanced internalization of
the DNA complexes.
Fig. 5: Primary human endothelial cells

(HUVECs) transfected with the reporter
vector pPK-CMV-R4 (see page 166)
using PromoFectin-HUVEC. Nuclei counterstained with DAPI.
Fig. 6: Primary human hepatocytes

transfected with the reporter vector
pPK-CMV-R4 (see page 166) using PromoFectin-Hepatocyte.
Fig. 7: Human monocyte-derived macrophages were transfected with a galactosidase-expressing plasmid after 7 days
of culture in the presence of 100 U/ml
GM-CSF and serum-containing medium .
1
2
Human Umbilical Vein Endothelial Cells (see page 13)

Human Dermal Microvascular Endothelial Cells (see page 17)
9 Cell Transfection
159
Cell Transfection
Fig. 8: Comparison of transfection efficiencies for PromoFectinHUVEC, PromoFectin, and three other common transfection reagents recommended for primary cells. Primary
HUVEC (see page 13) cultured in 24-well plates were transfected with a luciferase reporter plasmid*. The appropriate
protocols recommended by the manufacturers were used for
transfection with PromoFectin and the competing reagents.
All transfections were performed in triplicate (values normalized to 1 mg protein).
* See page 166 for reporter plasmids and page 107 for reporter assays.
PromoFectin-Neuron
PromoFectin-Neuron is a cationic polymer-based transfection reagent created
for the transfection of primary neuronal
cells, glial cells, and neuronal cell lines.
Cells take up the polymer/DNA complex
through endocytosis and the polymer is
biodegraded into non-toxic molecules.
Compared to viral methods, PromoFectinNeuron allows less complicated and
faster nucleic acid delivery with high
transfection rates.
PromoFectin-Insect
PromoFectin-Insect is a unique, lipsomebased transfection reagent optimized
for nucleic acid delivery into insect cells,
especially those derived from Spodoptera frugiperda (e.g. Sf9 or Sf21). It
outperforms other insect cell transfec-
Fig. 9: Primary neurons transfected with

the reporter vector pPK-CMV-R4 (see
page 166) using PromoFectin-Neuron.
tion reagents in comparison studies and

requires less DNA. PromoFectin-Insect
demonstrates broad vector delivery capability, excellent reproducibility, and
high expression levels for small- or largescale protein production.
a wide variety of cell types, including

problematic cell lines and primary cells. It
enables optimal gene silencing with low
cytoxicity.
PromoFectin-CellLine
PromoFectin-Polypeptide is a novel liposomal reagent created for the highly

efficient and fast delivery of proteins and
polypeptides (e.g. blocking peptides and
labeled antibodies) into a wide variety of
cells. It captures protein molecules and
transports them into the cytosol of living
cells while maintaining the proteins
structure and functionality. This allows
researchers to study intracellular protein
functions without the need for DNA
cloning and DNA transfection.
PromoFectin-Polypeptide is also available
in pre-coated single-use tubes, providing
additional convenience and speed.
PromoFectin-CellLine is a cationic lipidbased reagent developed to obtain optimal transfection results with several
widely used cell lines (CHO, HeLa, COS,
293, NIH-3T3) at low costs. Its formulation and the provided protocol allow fast
and convenient cell transfection.
PromoFectin-siRNA
PromoFectin-siRNA is a cationic lipidbased transfection reagent designed for
the highly efficient delivery of siRNA into
PromoFectin-Polypeptide
Fig. 10: Left: NIH-3T3 cells transfected with the GFP reporter plasmid pPK-CMV-R4
(see page 166) using PromoFectin-CellLine. Right: CHO cells transfected with the
GFP reporter plasmid pPK-CMV-R4 (see page 166) using PromoFectin-CellLine.
160
9 Cell Transfection
Cell Transfection
n Some cell types successfully transfected with PromoFectin
Adipocyte cells
Primary human pre-adipocytes
Endothelial-like cells
BBMC; BAEC; HLMECa, HUVECa, H-MVEC, primary human Lung Lymphatic Endothelial
cells, primary HUVECa, primary HDMECa
Epithelial-like cells
2CSFMEo, 3LL, 56FHTE8o, 6CSFMEo, 9HTEo-, A549, AsPC-1, BxPC-3, CaCo2, Ca Ski,
CFNPE90- CF, CFPEo, CFSMEo, CHO, D 407, DHD Pro.b, HeLa, Huh-7D12, IGROV1,
MCF-7, MDA-MB435S, MDCK, MLE-12, MLE-15, MRC-5, PGHAM-1, SAOS-2, SiHa,
SKOV3, U-2 OS, HEK293, HEK293FT
Fibroblast-like cells
BHK-21, COS-7, CV-1, L929, NIH 3T3, MEF, primary human skin fibroblasts (NHDF)
Hepatocyte-like cells
BNL, CL 2b, Hepa 1-6, HepG2b, Huh-7b, M-PAC, primary human/mouse/rat hepatocytesb
Keratinocytes
Primary human keratinocytes
Pancreatic carcinoma cells
AR42J, BxPC-3, MIAPaCa-2, PanC-1, PGHAM-1
Leukemia cells / lymphoblasts
Daudi, Jurkat, K562, Molt 4
Melanoma cells / melanocytes
B16-F10, primary human epidermal melanocytese
Myotubes / myoblasts / muscle cells
C2C12
Neuronal cells
CLBPEC, Neuro2A, PC12, SH-SY5Y, SHEP, SK-N-MC, F11, NG 108-15d, primary chicken
embryonic neurons, primary rat and mouse cortical neuronsd, primary rat hippocampal
neuronsd, primary rat cerebellar granular neuronsd, primary rat striatal neuronsd
Chondrocytes
HCS-2/8
Glioblastoma
U87
Blood cells
Human macrophagesc, monocyte-derived macrophagesc, RAW 264.7c, THP-1c
Mesenchymal stem cell
STRO-1+ DaMSCs cells
PromoFectin-HUVEC,
PromoFectin-Hepatocyte,
PromoFectin-Macrophage,
PromoFectin-Neuron,
Promofectin-siRNA
Please contact us or see www.promokine.info for information about new cell types that have been successfully transfected
using PromoFectin.
Product
Size
Catalog Number
PromoFectin
0.1 ml
0.5 ml
1 ml
PK-CT-2000-10
PK-CT-2000-50
PK-CT-2000-100
PromoFectin-HUVEC
0.1 ml
0.5 ml
PK-CT-2000-HUV-10
PK-CT-2000-HUV-50
0.1 ml
0.5 ml
PK-CT-2000-HEP-10
PK-CT-2000-HEP-50
0.1 ml
0.5 ml
PK-CT-2000-MAC-10
PK-CT-2000-MAC-50
0.15 ml
0.75 ml
5 x 0.75 ml
PK-CT-2000-NEU-015
PK-CT-2000-NEU-075
PK-CT-2000-NEU-375
0.5 ml
1 ml
PK-CT-2000-InC-50
PK-CT-2000-InC-100
0.75 ml
1.5 ml
PK-CT-2000-CL-075
PK-CT-2000-CL-150
0.2 ml
0.75 ml
5 x 0.75 ml
PK-CT-2000-RNA-50
PK-CT-2000-RNA-200
PK-CT-2000-RNA-1000
24 reactions
24 Single Use Vials
96 reactions
96 Single Use Vials
PK-CT-2000-POL-24
PK-CT-2000-POL-24Q
PK-CT-2000-POL-96
PK-CT-2000-POL-96Q
PromoFectin-Neuron
PromoFectin-Insect
PromoFectin-siRNA
9 Cell Transfection
161
9.2 Magnet Assisted Transfection (MATra)

Magnet Assisted Transfection
Transfection of adherent and suspen-

sion cells including primary cells
Suited for plasmids, oligonucleotides,
siRNA, and adenovirus/retrovirus
transfection
For stable and transient transfections
Also suited for high-thoughput
transfection
Adherent Cells
Suspension Cells
Magnet Assisted Lipofection

Adherent Cells
Use MATra-S
Immobilizer to make
the cells adherent.
Suspension Cells
Use MATra-S
Immobilizer to make
the cells adherent.
MATra-A
or MATra-si
Reagent
MA Lipofection Enhancer and lipofection

reagent of choice
Transfected
Cells
Transfected
Cells
Features

Easy, fast, and reproducible procedure

Functional with and without serum
and antibiotics
Very high transfection rates also with
many hard-to-transfect cell types
Up to several thousand fold increased
transgene expression
Very low amounts of nucleic acid
necessary for transfection
Magnet Assisted Transfection (MATra) is

a very fast, easy-to-handle, and highly
efficient technology for transfecting cells
in culture. It can be used for cells from a
broad variety of species. The major components of MATra are coated magnetic
nanoparticles, which effectively bind either nucleic acids or liposomes. Using a
special magnet plate, the nanoparticles
with the bound nucleic acids or liposomes are rapidly drawn towards the
cells and are deposited at a high concentration on the outer cell membrane.
Afterwards, the cells endocytose the
particles, which leads to a high transfection rate even with problematic cells.
MATra
nanoparticles
The MATra technology is distributed for IBA GmbH, Germany. For research use only!
Two approaches are possible:

Magnet Assisted Transfection using
MATra-A or MATra-siRNA Reagent.
Magnet Assisted Lipofection using
the MA Lipofection Enhancer and a
lipofection reagent of choice.
Both techniques can be used with adherent cells as well as with suspension cells.
For suspension cells, the cells have to be
immobilized at the bottom of the wells
to make use of the magnetic field. This
can be achieved by either temporarily
immobilizing the cells with MATra-S Immobilizer or by using modified culture
vessels. MATra is also suited for highthroughput transfection assays using
adapted protocols.
DNA
DNA + nanoparticle
complex
Magnet
Magnet Plate
Adherent cells
Fig. 11: Magnet Assisted Transfection (MATra) principle.
MATra-A Reagent
The MATra-A Reagent contains magnetic nanoparticles with an innovative
polyelectrolyte coating, which effectively
bind nucleic acids (e.g. plasmid DNA or
oligonucleotides) by means of electrostatic interactions. Using this technique,
nucleic acids are initially mixed with specifically coated magnetic nanoparticles.
The magnetic particles loaded with the
nucleic acids are then added to cells in
culture and a strong magnetic force is
applied beneath the cells. Exploiting this
strong magnetic field, the full nucleic
acid dose is rapidly drawn towards the
target cells on the bottom of the multiwell plate or cell culture flask. This results
in very close contact between the cell
membrane and the highly concentrated
nucleic acid loaded magnetic complexes
allowing optimal nucleic acid delivery.
MATra allows efficient transfection
without disturbing the cell membrane
architecture or leaving a hole in the cell
membrane as with other transfection
technologies. The transfection rate consequently is clearly increased - also with
difficult-to-transfect cells such as primary
cells. In addition, the amounts of nucleic
acids required for successful transfection
can be significantly reduced, which in
turn further reduces cytotoxicity.
Cell Transfection
Applications
162
9 Cell Transfection
GFP fluorescence
Overlay
Fig. 12: Mouse fibrosarcoma cells transfected with GFP plasmid using MATra (Data kindly provided for IBA GmbH by Dr. Lutz
Thon and Dr. Dieter Adam, Institute for Immunology, University Schleswig-Holstein, Kiel, Germany).
MATra-siRNA Reagent
siRNAs are small interfering/inhibitory
sequence-specific RNA molecules that
bind to a complementary sequence
of a target mRNA, block translation of
that target mRNA, and initiate its degradation. Consequently, synthesis of
the corresponding protein of interest is
prevented and the specific gene function
is knocked-out. This process is termed
RNA interference or gene-silencing and
it is used to elucidate gene functions.
Specifically designed small inhibitory

RNA oligonucleotides (siRNAs) allow
transient inhibition of gene expression in
vitro and in vivo.
MATra-siRNA contains specifically coated magnetic nanoparticles, which are
optimized to effectively bind and stabilize siRNA. It provides optimal delivery
of siRNA into many different cell types
enabling efficient and prolonged genesilencing.
Fig. 13: Human endometrial stromal cells

transfected with fluorescein-labeled
siRNA using MATra-siRNA Reagent
(Data kindly provided for IBA GmbH by
Dr. Birgit Gellersen, Endokrinologikum
Hamburg, Germany).
%GFP positive cells

50
40
30
20
* See page 166 for reporter plasmids.
Reagent LF
Reagent S18
Reagent FG6
10
PromoFectin-HUVEC
Fig. 14: Comparison of transfection efficiencies of MATra,

PromoFectin-HUVEC, and three other common transfection
reagents recommended for primary cells. Primary human
dermal microvascular endothelial cells (HDMEC, see page
17) cultured in 24-well plates were transfected with a GFP
reporter plasmid*. The appropriate protocols recommended
by the manufacturers were used for transfection with MATra,
PromoFectin, and the competing reagents. All transfections
were performed in triplicate.
MATra
Cell Transfection
Phase contrast
9 Cell Transfection
The MA Lipofection Enhancer contains

coated magnetic nanoparticles which
bind liposomes and any polycationic
transfection reagents (e.g. PromoFectin)
which in turn bind nucleic acids (e.g.
plasmid DNA, oligonucleotides, siRNA).
Using the MA Lipofection Enhancer, cells
are transfected more efficiently than
with liposomal or polycationic transfection reagents alone.
MATra-S Immobilizer
Suspension cells have to be made adherent first to perform Magnet Assisted
Transfection (as well as many other conventional transfection procedures). The

MATra-S Immobilizer contains coated
magnetic nanoparticles which bind suspension cells. Using a special magnet
plate, the magnetic nanoparticles with
the bound cells are rapidly drawn towards the bottom of the cell culture vessel where they are immobilized. By combining the MATra-S Immobilizer with the
other MATra reagents, suspension cells
can be transfected conveniently.
See www.promokine.info for a listing of more than 100 cell types
successfully transfected using Magnet Assisted Transfection and Magnet
Assisted Lipofection.
Liposome
Cell membrane
Magnetic nanoparticles
Fig. 15: Principle of Magnet Assisted

Lipofection. The MA Lipofection
Enhancer can be combined with any
commercially available transfection
reagent based on polycations or lipids.
Response (expression level)

200
150
MATra
100
Fig. 16: Magnet Assisted Transfection and lipofection in comparison. Luciferase expression was assayed after 24 hours.
With MATra reagents, low vector doses are sufficient to obtain good results.
50
2.
tion
genera
fection reagents
0.05
0.1
%GFP positive cells

10
8
6
4
Reagent LF
Reagent LF +
MA Lipopfection Enhancer
IBAfect
IBAfect +
MA Lipopfection
Enhancer
* See page 166 for reporter plasmids.
nts
reage
1. generation lipo
Dose (g DNA)
Fig. 17: Comparison of transfection efficiencies of two lipofection reagents with and without MA Lipofection Enhancer. Primary human dermal microvascular endothelial
cells (HDMEC, see page 17) cultured in 24-well plates were
transfected with a GFP reporter plasmid*. The appropriate
protocols recommended by the manufacturers were used for
transfection. All transfections were performed in triplicate.
IBAfect liposomal transfection reagent is also available at
PromoKine. Please visit www.promokine.info for more information.
ction
lipofe
0.15
Cell Transfection
MA Lipofection Enhancer
163
164
9 Cell Transfection
Magnet Plates
24 Magnet Bar Plate (8 x 12 cm):

optimized for 24-well plates.
96 Magnet Bar Plate (8 x 12 cm):
optimized for 96-well plates
Also available: Magnet Bar Plate Sets.
Cell Transfection
To perform Magnet Assisted Transfection, specially designed neodymium

magnet plates are necessary. PromoKine
offers them in different sizes, optimized
for different cell culture vessels.
Universal Magnet Plate (8 x 13 cm):

suitable for 6-well, 12-well, 24-well,
48-well, or 96-well plates, T75 flasks, 60
mm dishes etc.
Universal Magnet Plate (26 x 26 cm):
suitable for 6-well, 12-well, 24-well,
48-well, or 96-well plates, T75 flasks,
60 mm dishes, 100 mm dishes etc.
Fig. 18: Universal magnet plate (8 x 13 cm) with collection of different culture plates
and MATra reagents.
Fig. 19: 96 magnet bar plate (8 x 12 cm)

with 96-well culture plate.
Product
Size
Catalog Number
MATra-A Reagent
0.05 ml
0.2 ml
1 ml
PK-CT-2001-005
PK-CT-2001-020
PK-CT-2001-100
MATra-siRNA Reagent
0.05 ml
0.2 ml
1 ml
PK-CT-2021-005
PK-CT-2021-020
PK-CT-2021-100
0.05 ml
0.2 ml
1 ml
PK-CT-2003-005
PK-CT-2003-020
PK-CT-2003-100
MATra-S Immobilizer
0.05 ml
0.2 ml
1 ml
PK-CT-2002-005
PK-CT-2002-020
PK-CT-2002-100
Universal Magnet Plate, 8 x 13 cm plate

Universal Magnet Plate, 26 x 26 cm plate
24 Magnet Bar Plate, 8 x 12 cm plate
96 Magnet Bar Plate, 8 x 12 cm plate
PK-CT-2011-000
PK-CT-2012-000
PK-CT-2006-000
PK-CT-2004-000
Four 24 Magnet Bar Plates

Four 96 Magnet Bar Plates
Two 24 and two 96 Magnet Bar Plates
PK-CT-2008-000
PK-CT-2009-000
PK-CT-2010-000
Magnet Plate
Set of four Magnet Bar Plates
Note: Please contact us to request a test offer for MATra.
9 Cell Transfection
165
9.3 Transfection Accessories
Applications
Enhancement of gene expression in
transfected cells
Fast and easy protocol

Optimized conditions for popular
cell lines
Efficient for both transient and
stable transfections
Features

Up to 12-fold increased gene

expression levels in diverse cell
lines after transfection
Compatible with all commonly used
cell transfection reagents
The GeneBooster Reagents are chemical cocktails designed to significantly

enhance gene expression levels in transfected cells. Three unique formulations
have been developed for different cell
types. Simply add GeneBooster reagent
to the culture medium four hours after

transfection and an increase in expression levels up to 12-fold can be
achieved. The reagents can be used in
combination with all commercially available DNA transfection reagents (including all PromoFectin Reagents). One vial
of GeneBooster Reagent is sufficient for
150 transfections using 1 ml transfection
volume.
The three GeneBooster Reagents are
also available in a kit.
Fig. 20: K562, HeLa-S3, MCF7, and P19 cells were transfected with the beta-Gal reporter plasmid pPK-CMV-R1 (see page 166).
After 4 hours, the appropriate GeneBooster reagent was added to the cells which were stained 48 hours post transfection using a
beta-Gal Staining Kit (see page 107) and examined under a microscope.
9.3.2 Cell Clean

Applications
Highly efficient cleanup of
transfected cells
Features
Efficient removal of extracellular lipid/
DNA complexes after cell transfection
Non-toxic to cells
Fast and easy procedure
Better downstream results post
transfection
CellClean is a unique washing buffer that
has been designed to remove all com-
plexes of DNA and cationic lipids that

associate with cell surfaces during transfection. It shows no cytotoxicity and can
distinguish between extracellular and
intracellular plasmid DNA. When CellClean is used as part of the cell washing
procedure, more precise quantitation of
the amount of DNA delivered into cells
can be achieved. Moreover, it improves
downstream results such as DNA stability, cytometry, and gene delivery studies.
Fig. 21: Adherent cells were incubated with cationic lipid/DNA complexes for 4 hours
and washed with PBS (lane 1) or CellClean (lane 2). Cells were lysed, the DNA extracted and analysed by agarose gel electrophoresis. Arrows show extracellular plasmid
DNA that could be removed from cell surfaces when using Cell Clean.
Product
Size
Catalog Number
GeneBooster Reagent I
3 vials x 1.5 ml each
PK-CT-2020-1
GeneBooster Reagent II
PK-CT-2020-2
GeneBooster Reagent III
PK-CT-2020-3
GeneBooster Reagent Kit
PK-CT-2020-0
100 ml
PK-CT-1000-01
CellClean
Cell Transfection
9.3.1 GeneBooster
166
9 Cell Transfection
Cell Transfection
9.4 Reporter, Expression and Fusion Vectors

Applications
Features
Transient high-level expression of five

popular reporter genes
Constitutive high-level target gene ex pression yielding native or HA-tagged
proteins
Constitutive high-level GFP or Lucifer-
ase fusion protein expression
Monitoring transfection efficiency and
gene expression
Creating GFP or Luciferase expressing
stable cell lines
Optimized CMV promoter provides

high level gene expression
Suited for a wide variety of cell types
for both stable and transient gene ex-
pression
Small vector sizes ensure high transfec-
tion efficiency and high plasmid yield
in E. coli
Convenient multiple cloning site with
most commonly-used restriction sites
for optimized cloning and expression
of target genes
The pPK-CMV Vectors contain a modified human cytomegalovirus (CMV)

based promoter/enhancer sequence that
has been optimized for high level gene
expression. Sequence motives that limit
expression have been removed, while
others that promote high expression
levels have been retained. Consequently,
these vectors yield significantly higher
expression levels than other expression
vectors based on the standard CMV
promoter. In addition, the plasmid backbones are optimized to allow for very
small vector sizes and higher plasmid
yields when amplified in E. coli.
Please check our website at www.promokine.info for details and prices.
9.4.1 pPK-CMV Reporter Vectors

The pPK-CMV Reporter Vectors have
been designed for high level, transient
expression of reporter or target genes in
a wide variety of mammalian cells and
tissues (in vitro or in vivo). The vectors
are available without a reporter gene
to clone and express a gene of interest
(blank vector) as well as with the following reporter genes: -galactosidase

(lacZ), green fluorescent protein (GFP),
luciferase (Luc), chloramphenicol acetyltransferase (CAT), and secreted alkaline
phosphatase (SEAP).
See also page 107/108 for luciferase,

beta-galactosidase and GFP reporter
assays.
Plasmid maps, multiple cloning sites, and

DNA sequences are available on our website.
9.4.2 pPK-CMV Expression Vectors

The pPK-CMV Expression vectors have
been designed for constitutive high-level
transgene expression. Three versions
of the pPK-CMV Expression Vector are
available: pPK-CMV-E1 does not have
an expression tag. It is intended for
expressing recombinant proteins in the
native forms. pPK-CMV-E2 comes with

an N-terminal HA (hemagglutinin) fusion tag. pPK-CMV-E3 comes with a
C-terminal HA-fusion tag. The HA fusion
tags allow easy detection and purification of proteins.
All three Expression Vectors come with a

positive control vector containing a CAT
gene.
multiple cloning sites upstream or downstream of the reporter gene.

Four versions of the fusion vector are
available for convenient and efficient
gene cloning and expression of the target gene fused with a reporter gene:
pPK-CMV-F1 (C-GFP) has a GFP coding sequence with a stop codon inframe
in the MCS. The gene of interest can
therefore be expressed with GFP at the
C-terminus. pPK-CMV-F2 (N-GFP) has
a GFP coding sequence without a stop
codon inframe in the MCS. The gene
of interest can therefore be expressed
with GFP at the N-terminus. pPK-CMVF3 (C-Luc) has a Luciferase coding se-
quence with a stop codon inframe in the

MCS. The gene of interest can therefore
be expressed with Luciferase at the Cterminus.
pPK-CMV-F4 (N-Luc) has a Luciferase
coding sequence without a stop codon
inframe in the MCS. The gene of interest
can therefore be expressed with Luciferase at the N-terminus.
9.4.3 pPK-CMV Fusion Vectors

The pPK-CMV Fusion Vectors have been
designed for constitutive high-level expression of GFP or Luciferase fusion
proteins and efficient selection in both
E. coli (using kanamycin) and mammalian cells (using neomycin). An SV40
polyadenylation signal provides efficient
transcription termination and polyadenylation - thus ensuring stable mRNA. A
pUC origin of replication allows for high
copy numbers in E. coli.
The vectors can be used for either
transient transfection or for generating
stable cell lines expressing only the reporter gene or the fusion target-reporter
gene construct. The vectors contain two
Please check our website at www.promokine.info for details and prices.
See also page 107/108 for luciferase

and GFP reporter assays.

Antibodies have a broad range of applications like the detection of cellular structures as well as the specific identification,
localization, and purification of intracellular and extracellular
biomolecules such as proteins. They are also essential tools in
describing cellular processes and for diagnostic purposes such
as ELISAs. Consequently, they are important for many research
disciplines like apoptosis, signal transduction, neurobiology,
virology, and tumor biology.
10
10

10.1 Mono- and Polyclonal Antibodies . . . . . . . 169
10.2 Antigens and Cell Lysates . . . . . . . . . . . . . 172
10.3 Labeled Antibodies and Labeling Kits . . . . 173
10.4 ELISA Kits . . . . . . . . . . . . . . . . . . . . . . . . . 175
10 Antibodies and ELISAs
169
10.1 Mono- and Polyclonal Antibodies

ies, immunofluorescence microscopy,
immunohistochemistry, immunoprecipitation or Westem blotting. The antibodies have been produced in several different hosts (e.g. rabbit, mouse, rat, goat)
and show specific reactivities against
antigens from different organisms (e.g.
human, mouse, rat). For many of our
antibodies, blocking peptides as well as
cell and tissue lysates are available that
can be used for functional studies or as
positive controls (see page 172). Other
primary antibodies are also available as

conjugates with e.g. FITC or biotin.
PromoKine lists several thousand
primary antibodies on its website. Please
visit www.promokine.info/antibodies to
find your specific antibody of interest using our convenient search function.
PromoKine provides a variety of primary
antibodies for many different research
topics:
n Cell Signaling: Cytokines, Growth Factors, CD Antigens, soluble Receptors, and Ligands
Numerous antibodies against Interleukins (ILs), Interferons (IFNs), Fibroblast
Growth Factors (FGFs), Monocyte
Chemotactic Proteins (MCPs), Macrophage Inflammatory Proteins (MIPs),
Transforming Growth Factors (TGFs),
Tumor Necrosis Factors (TNFs), Vascular Endothelial Growth Factors and
Receptors (VEGFs, VEGFRs), InsulinLike Growth Factors and Binding Proteins (IGFs, IGF-BPs), Platelet-Derived
Growth Factors (PDGFs), CC Chemokine
Receptors (CCRs), Bone Morphogenetic
Proteins (BMPs), Matrix Metalloproteinases (MMPs), Neurotrophins (NTs),
Stromal Cell-Derived Factors (SDFs),
beta-Defensins (BDs), Eotaxins, diverse

CD antigens, WNTs as well as Adiponectin, BAFF, EGF, G-CSF, GM-CSF, M-CSF,
HGF, EPO, Insulin, Leptin, PLGF, Resistin,
RANKL, RANTES, TIMP, TPO and many
more targets.
Please see www.promokine.info/antibodies/cell-signaling

n Signal Transduction
Numerous antibodies against Akt-1,
ATF-6, BAP29 & BAP36, CIKS, CTRP,
DARP, GADPH, Hrk, IKAP, IKKs, IRAKs,
JNK1, MADD, MAPKAP1, MyD88,
Nanos, PAK, PTEN, Raptor, SATB1 &

SATB2, SHP2, Ski, Slug, SOCS1, STAT1,
Sumo, TOR, TRAF, TRPC, TSC, Wnt-10,
XBP-1, XEDAR and many more targets.
Please see www.promokine.info/antibodies/signal-transduction

n Apoptosis
Numerous antibodies against Caspases, Calpains, Death Receptors (DRs),
HDACs and HATs as well as Acinus, AIF,
Annexin V, Apaf-1, APO-1/Fas, ASK1,
Bad, Bax, Bcl-2, Bid, CAD, CARD, CIDE,

Cytochrome c, Daxx, DcR, DFF, DRAK,
DRAM, Fas Ligand, FLASH, FLIP, ICAD,
ILPIP, LAMP, NALP, NOD2, PDCD,
Please see www.promokine.info/antibodies/apoptosis

n p53 Network
Antibodies e.g. against p53, p53DINP1/
SIP, p53R2, PARC, PERP, PID/MTA2,
ChK2, and MTBP.
Please see www.promokine.info/antibodies/p53-network
PHAP, PIST, PUMA, RAIDD, RICK, RIP3,

Smac, SODD, Survivin, TACE, TCCR,
TIGAR, TRAIL, TWEAK, XAF, XIAP, ZIPK
and many more targets.
Antibodies and
ELISAs
PromoKine offers a wide and continuously growing range of mono- and

polyclonal antibodies against a variety
of interesting molecules like cytokines
and growth factors as well as many
other important key targets in immunology, signal transduction, apoptosis,
neurobiology, virology, tumor biology,
autophagy, homeostasis etc. All antibodies are purified and highly active and can
be used for diverse applications such as
ELISAs, flow cytometry, functional stud-
170
n Neurobiology
Antibodies and
ELISAs
Numerous antibodies against APH1,

APP, BACE, BRSK, CAPS, Clusterin/
ApoJ, CDNF, CRMP1, DISC1, FEZ,
GFR-alpha, Grik, KIF5, KLHL1, LGI,

MANF, Neuritin, Neurotrypsin, Neurturin, Nicastrin, NK3R, NPAS3, PEN2,
SAPAP, Slitrk, Syntaphilin, TDP43,

TMP21, TOCA and many other targets.
Please see www.promokine.info/antibodies/neurobiology

n Stem Cell Research
Numerous antibodies against Bad, betaActin, ADAM10, Adiponectin, Akt1/PKB,
Bone Morphogenetic Proteins (BMPs),
CD44, CD90, CD105, CXCR4, DKK,
Dnmt2, Elk-1, Eotaxin, Erk2, FGF-2,
GDF-9, GM-CSF, GRO-beta, HDACs,

IFN-gamma, IKK-alpha, IL-1, IL-3, IL-6,
IL-8, IL-12, IL-13, Lck, LRP-5/6, MCP-1,
MEK1, MIP-1, MIP-2, NFkB, Nanog,
Notch 1, Oct-4, PDGFR, PPAR-gamma,
PTEN, Raf-1, RANTES, SCF, SDF-1,

Smad, Sox, SSEA, Stat, Synapsin, Tau,
TGF-beta, TLR, TRAF6, Tubulin, VEGF,
Vimentin, Wnt and many other targets.
Please see www.promokine.info/antibodies/stem-cell-research

n Cancer Research
Numerous antibodies against BRCA,
Cathepsins, CD26, CD44, CUEDC,
EMAP-2, FAP, Galectin-3, HEC-1,
ICAM-1, IRF7, JMJD, MART-1, Matrix

Metalloproteinases (MMPs), MnSOD,
PARC, PAX6, Podoplanin, PTEN, OCIAD,
SIPA1L, SRC3, TIMP-1, TNF-alpha &

TNF-beta, VCAM-1, VEGF and many
more targets.
Please see www.promokine.info/antibodies/cancer-research

n Virology
Numerous antibodies against HIV and
Chemokine Receptors (e.g. CCRs,
CX3CR1, CXCR4, DC-SIGN, DDX-3,
HIV-I and HIV-II Glycoproteins, TRIM5),
Herpes (e.g. TNFRSF14/HVEM), SARS
and SARS Receptors (e.g. ACE2, SARS E,
SARS M, SARS Spike, SARS-NP), Anthrax

(e.g. Anthrax EF, Anthrax LF, Anthrax
PA, ATR/TEM8), West Nile Virus (e.g.
WNV Core, WNV Env, WNV Matrix),
Influenza (e.g. Influenza-A and Influenza-B), Avian Influenza (e.g. AFHA, H5N1
Neuramidase and Hemagglutinin, H5N1

NS1/NS2), Hepatitis (e.g. HCVNS3,
HDV, Hepatitis B), Epstein-Barr and
Dengue Virus, (e.g. EBV, Dengue type 2)
and virus-related targets (e.g. Rift Valley
Fever Virus, UNG1, UNG2).
Please see www.promokine.info/antibodies/virology

n Toll-Like Receptor Related
Numerous antibodies against Toll-Like
Receptors (TLRs, TLR1-TLR11) as well
as BTK, ECSIT, MD-1 & MD-2, RIG-1,

SARM, SIGIRR, ST2, TAB1, TIRAP, TIRP,
TOLLIP, TRIAD3A, TRIF, UBC13 and

many other targets.
Please see www.promokine.info/antibodies/toll-like-receptor-related

n Homeostasis
Numerous antibodies against ATM,
beta-Actin, Tumor Endothelial Markers
(TEMs), BRCC, CLIP170, CSN8, EVER,
LIMP2, LSD1, MORF4, Nephrin, op18,

PTK7, RAP80, RGP1, RTP801, SCO, Slc,
STEAP, TNPO3, TP1, TRIM25, VPS53
Please see www.promokine.info/antibodies/homeostasis
and many more targets.
171
n Kinases & Phosphatases

IKK, JIK, JKK, Lck, LIMK, LOK, LOX-1,
MAPKAPK-2, Mek, MST-1, NIK, PAK,
PDK1, PKA, PKC, PTP-1B, Raf1, RIPK3,
Please see www.promokine.info/antibodies/kinases-and-phosphatases

n Phospho-specific Antibodies
Numerous antibodies against phosphorylated AFX, Akt, ATF2, Bad, BRCA1,
c-Jun, c-Myc, Elk-1, Erk1/2, FAK, GSK-3,
IRS, JKK, Lck, MAPKAPK-2, Mek1/2,

MSK1, PKC, Raf, Stat, Tau, TrkA, VASP
and many other targets.
Please see www.promokine.info/antibodies/phospho-specific-antibodies

n Immunology
Many antibodies e.g. against ADAP,
Blimp-1, Carabin, CRTH2, IFN-beta,
IL-33, MICA, ORAI, STIM2, TSLP and

TSLP Receptor.
Please see www.promokine.info/antibodies/immunology

n Innate Immunity
Many antibodies e.g. against Cathelicidin, IPR1, LGP2, MDA5, SLPI, VISA,
Unc93b, and ZBP1.
Please see www.promokine.info/antibodies/innate-immunity

n Autophagy
Antibodies e.g. against Ambra1, APG5
& APG7, Apo-1/Fas, ATG, Bad, Bax,
Bcl-2, Bid, Bnip3L, DRAM, IRGM, IRS-1,

LAMP, Mek1, PIST, Presenilin, and PTEN.
Please see www.promokine.info/antibodies/autophagy

n Subcellular Markers
Antibodies e.g. against Apaf-1, Bad, Bax,
Bcl-6, Bcl-xL, Bid, CREB, DKK1, Hsp90,
Oct, Syndecan-4, Toll-like Receptors

(TLRs), Tubulin, and VDAC.
Please see www.promokine.info/antibodies/subcellular-markers
ROCK-1, SEK1, SLK, Trk and many other

targets.
Antibodies and
ELISAs
Numerous antibodies against AAK1,

Akt/PKB, ALK, AMPK, ASK1, BLK, Brk,
Chk2, DNA-PK, Erk, FAK, HER2/ErbB2,
172
n Anti-Tag Antibodies
Antibodies and
ELISAs
Many antibodies e.g. against FLAG-Tag,

GFP, RFP, GST-Tag, HA-Tag, His-Tag,
Myc-Tag, and MBP.
Please see www.promokine.info/antibodies/anti-tag-antibodies

n Infectious Diseases
Antibodies e.g. against Anthrax, Chlamydia LPS, or Neisseria gonorrhea.
Please see www.promokine.info/antibodies/infectious-diseases

n Obesity
Antibodies e.g. against ELOVL6, GPAT1,
Lipe/HSL and UROP11.
Please see www.promokine.info/antibodies/obesity

n Miscellaneous Antibodies
Antibodies e.g. against Cox, Growth
Hormone (GH), HEP-1, Immunglobu-
lins, Luciferase, Megalin, MOG, MPO,

Plectin, and Syndecan.
Please see www.promokine.info/antibodies/miscellaneous-antibodies
10.2 Antigens and Cell Lysates

The PromoKine peptides are perfectly
suited as blocking reagent in combination with the PromoKine antibodies and
as a control in e.g. immunoblotting/
Western blot, ELISA, or immunohistochemistry assays.
PromoKine also provides a wide range
of ready-to-use cell and tissue lysates

that can be used as controls in e.g. gel
electrophoresis, Western blotting, or immunoprecipitation. Moreover, they are
also useful in enzymatic activity analysis,
protein-protein interaction analysis, and
tissue specific expression identification.
The total tissue and cell line protein lysates are available from human, mouse,
and rat origin as well as from a variety
of developmental stages. Furthermore,
beside lysates from normal tissue also
lysates from tumor tissue and diseased
tissue are available.
For a complete list of our peptides and cell lysates please see www.promokine.info/antigens-and-cell-lysates
or the PromoKine Price List.
173
10.3 Labeled Antibodies and Labeling Kits

n Labeled Secondary Antibodies

(HRP), alkaline phosphatase (AP), or
biotin. All antibodies are purified and
highly active, and they can be used for
Product
Product Description
Donkey Anti-Goat antibody (pAb),

Fluorescein-coupled
diverse applications including ELISAs,

immunofluorescence microscopy, immunohistochemistry, and Western blotting.
Size
Catalog Number*
Donkey Anti-Goat IgG conjugated to Fluorescein

(H&L)
2 mg
PK-AB718-9360
Donkey Anti-Goat antibody (pAb),

Texas Red-coupled
Donkey Anti-Goat IgG conjugated to Texas Red (H&L)
2 mg
PK-AB718-9370
Goat Anti-Chicken antibody (pAb),

Texas Red-coupled
Goat Anti-Chicken IgG conjugated to Texas Red (H&L)
2 mg
PK-AB718-9170
Goat Anti-Mouse antibody (pAb),

AP-coupled
Goat Anti-Mouse IgG conjugated to Alkaline

Phosphatase (H&L, minimum cross-reactivity)
1 mg
PK-AB718-9780

Biotin-coupled
Goat Anti-Mouse IgG conjugated to Biotin

(H&L, minimum cross-reactivity)
2 mg
PK-AB718-9740

Fluorescein-coupled
Goat Anti-Mouse IgG conjugated to Fluorescein

2 mg
PK-AB718-9760

HRP-coupled
Goat Anti-Mouse IgG conjugated to Horseradish

Peroxidase (H&L, minimum cross-reactivity)
2 mg
PK-AB718-9720

Texas Red-coupled
Goat Anti-Mouse IgG conjugated to Texas Red (H&L)
2 mg
PK-AB718-9770
Goat Anti-Rabbit antibody (pAb),

AP-coupled
Goat Anti-Rabbit IgG conjugated to Alkaline

1 mg
PK-AB718-9980

Biotin-coupled
Goat Anti-Rabbit IgG conjugated to Biotin

2 mg
PK-AB718-9940

Fluorescein-coupled
Goat Anti-Rabbit IgG conjugated to Fluorescein

2 mg
PK-AB718-9960

HRP-coupled
Goat Anti-Rabbit IgG conjugated to Horseradish

2 mg
PK-AB718-9920

Texas Red-coupled
Goat Anti-Rabbit IgG conjugated to Texas Red (H&L)
2 mg
PK-AB718-9970

HRP-coupled
Goat Anti-Rabbit IgG conjugated to horseradish

peroxidase
0.5 ml
PK-AB577-6401
Goat Anti-Rat antibody (pAb),

AP-coupled
Goat Anti-Rat IgG conjugated to Alkaline Phosphatase

1 mg
PK-AB718-9580
Goat Anti-Rat antibody (pAb),

HRP-coupled
Goat Anti-Rat IgG conjugated to Horseradish

2 mg
PK-AB718-9520
PF-XXX-Goat Anti-Human IgG
Conjugated to PromoFluor dye of choice
1 mg
PK-PFXXX-AK-H1
PF-XXX-Goat Anti-Mouse IgG
1 mg
PK-PFXXX-AK-M1
PF-XXX-Goat Anti-Rabbit IgG
1 mg
PK-PFXXX-AK-R1
Rabbit Anti-Chicken antibody

(pAb), AP-coupled
Rabbit Anti-Chicken IgG conjugated to

Alkaline Phosphatase (H&L)
1 mg
PK-AB718-9180
Rabbit Anti-Chicken antibody

(pAb), HRP-coupled
Rabbit Anti-Chicken IgG conjugated to Horseradish

1.5 mg
PK-AB718-9120
Rabbit Anti-Goat antibody (pAb),

AP-coupled
Rabbit Anti-Goat IgG conjugated to Alkaline

0.5 mg
PK-AB718-9380

HRP-coupled
Rabbit Anti-Goat IgG conjugated to Horseradish

1 mg
PK-AB718-9320
Note: Please see our website for an up-to-date list of available labeled antibodies.
*XXX- stands for the respective PromoFluor dye (e.g. PF-350, PF-405, etc.; see page 146).
Antibodies and
ELISAs
PromoKine is offering secondary antibodies which are fluorescently labeled

or conjugated to horseradish peroxidase
174
Antibodies and
ELISAs
Product
Product Description
Size
Catalog Number

HRP-coupled
Rabbit Anti-Goat IgG conjugated to horseradish

peroxidase
0.5 ml
PK-AB577-6403
Rabbit Anti-Mouse antibody (pAb),

HRP-coupled
Rabbit Anti-Mouse IgG conjugated to horseradish

peroxidase
0.5 ml
PK-AB577-6402
n Protein and Antibody Labeling Kits

PromoKine also offers ready-to-use
Protein and Antibody Labeling Kits.
These innovative kits have been
developed for convenient and highly

efficient labeling of specific antibodies or target proteins with our superior
Product
Product Description
PromoFluor dyes (see page 145).
Size
Catalog Number*
Kit for Labeling of Proteins/Antibodies

with a Selected PromoFluor Dye
5 reactions
PK-PF-LK-XXX-05
Kit for Labeling of Proteins/Antibodies

with a Selected PromoFluor Dye
10 reactions
PK-PF-LK-XXX-10
n ECL Chemiluminescence Detection Kit

High resolution and sensitivity (picogram
to femtogram amounts of target)
Stable hard copy results on film
Long signal duration enables multiple
exposures
Only small amounts of antibody
required for strong signals
Detection possible with short
exposure times (minutes)
Excellent price to performance ratio
PromoKines ECL Chemiluminescence

Detection Kit is a convenient, non-radioactive detection system containing all
the reagents required for highly sensitive
chemiluminescent detection of immobilized proteins (Western blotting) or immobilized nucleic acids (Southern Blotting
or Northern Blotting) conjugated with
HRP (Horse Radish Peroxidase). This
ready-to-use kit allows the detection of
Product
Product Description
ECL Chemiluminescence Detection

Kit
Kit for Chemiluminescent Detection of HRP

or HRP-labeled Biomolecules
membrane immobilized specific antigens

or nucleic acid sequencesdirectly labeled
with HRP or indirectly labeled with HRPtagged antibodies/streptavidin/biotin.
Size
Catalog Number
120 ml
500 ml
1000 ml
PK-MB902-500-120
PK-MB902-500-500
PK-MB902-500-1000
Also available: Prestained RTU Protein Ladders, Fluorescent FluoProtein Gel Stain and GelQuick Protein Gel
Stain/Destaining Kit (see page 123).
*XXX- stands fo the respective PromoFluor dye (PF-405, PF-415, PF-488P, PF-488LSS, PF-514LSS, PF-546, PF-555, PF-633, PF-647, PF-670, PF-750,
PF-770; see page 146) Please see our website for an up-to-date list of available PromoFluor Labeling Kits.
175
10.4 ELISA Kits

The ready-to-use ELISA kits are carefully
validated and come with a straightforward and easy-to-use manual as well as
all reagents required to perform a convenient ELISA with reliable and reproducible results. The 96-well assay plate
can either be used at once or a desired
number of assays can be used separately
as the wells in the assay plates can be
detached.
economical in medium- to high-throughput applications. The EDKs are intended

for experienced ELISA users and contain
all required reagents to assay your target
protein/peptide in ten 96-well microtiter
plates (approximately 1,000 assays).
We continuously add new ELISA kits to
our inventory.
For an up-to-date product list please
visit www.promokine.info/ELISAs
In addition, we offer ELISA Development

Kits (EDKs, see page 176) which are very
n Cell Signaling: Cytokines, Growth Factors, Receptors and Ligands

ELISAs for detection and quantification of human, mouse, rat, monkey, and pig:
Interleukins
e.g. IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, IL-7, IL-8, IL-12, IL-13, IL-17, IL-22, and
IL-33
Growth Factors & Colony Stimulating

Factors
e.g. BDNF, CTGF, EGF, FGFs (including FGF-2), G-CSF, GM-CSF, HGF, IGF-1, IGF-BPs,
M-CSF, beta-NGF, PDGF-BB, TGF-beta, VEGF-A, and VEGF-C
Interferons
e.g. IFN-alpha, IFN-gamma
Chemokines
e.g. CRG-2, CTACK, Eotaxin-2, Fractalkine, GRO-alpha, GRO-beta, HCC-4, IP-10,

MCP-1, MCP-2, MCP-5, MDC, MIG, NAP-2, LIX, MIP-1, MIP-3, PARC, RANTES, and
SDF-1 alpha
Tumor Necrosis Factors
e.g. TNF-alpha and TNF-beta
We also supply ELISAs for Angiogenin,

Angiopoietin-1, Angiostatin, BAFF, Bone
Morphogenetic Proteins (BMPs), CRP,
Matrix Metalloproteinases (MMPs),
EPO, flt-3 Ligand, Galectins, ICAMs,

Leptin, LIF, MPO, MSP, Myostatin,
PAI-1, Resistin, sRANKL, SCF, Selectins,
TNF Receptors, TIMP-1 & TIMP-2, TPO,
Please see our website for a complete list of our Cell Signaling ELISAs.
n CD Marker
ELISAs for detection and quantification
of human, mouse and monkey: CD8,
CD23, CD26, CD28, sCD30, CD30L,
CD31, CD40, CD40L, CD44, CD50,

CD54, CD62, CD102, CD106, CD134,
CD137, CD144, CD154, and CD162.
Please see our website for a complete list of our CD ELISAs.

n Hormones
ELISAs for detection and quantification
of human Relaxin, Thymosin alpha-1,
Thymosin beta-4, and Thymulin.
Please see our website for an up-to-date list of our Hormone ELISAs.
VCAM-1, VEGF Receptors and many

more interesting targets.
Antibodies and
ELISAs
PromoKine offers a range of highly sensitive and specific ELISA kits that meet
optimal quality standards. They can be
used for accurate and consistent detection and quantitation of numerous
interesting biomolecules in cell biology
research and are applicable for different
sample material (e.g. cell culture supernatant, serum and plasma) from various
mammalian organisms (human, mouse,
rat, monkey or pig). Some ELISAs can
also be used with cell/tissue lysates,
urine or stool samples.
176
n Apoptosis
Antibodies and
ELISAs
ELISAs for detection and quantification of human and monkey:

Annexin V, APO-1/Fas, APRIL, BAFF,
Bcl-2, Caspase-8 & Caspase-9, Cathepsin

K & Cathepsin L, Cytochrome c, DcR3,
Granzyme A & Granzyme B, LIGHT,
OPG, OX40, TRAIL, VE-Cadherin and

many more interesting targets.
Please see our website for a complete list of our Apoptosis ELISAs.
n Other ELISAs
Here you will find a range of ELISAs for
target molecules which play important
roles in oxidative cell stress and inflammation as well as different metabolism
patways and diseases in the skeletal,
cardiovascular and renal systems. Other
ELISAs included herein can be used for
detecting and quantifying targets which
are essential markers e.g. in gastroenterology, fertility, diabetes, oncology,

clinical immunology and orthomolecular
medicine: ADMA, Adiponectin, Calprotectin, CRP, Myostatin, NT-proCNP,
NT-proANP, Leptin, Matrix Metalloproteinases (MMPs), Osteonectin,
Osteoprotegerin, Oxidized LDL, PAI-1,
sRANKL, RANTES, Thyreoglobulin, Urotensin II, VDBP, Vitamin D, Zonulin and

many more interesting targets.
Please see our website at www.promokine.info/ELISAs for a complete list.

n ELISA Development Kits
Each of PromoKines ELISA Development Kits (EDKs) contains the following components to detect and quantify
the target molecule in at least 10 x 96
ELISA plate wells: Capture antibody,
(biotinylated) detection antibody, avidinperoxidase conjugate and a thoroughly

calibrated standard. A huge variety of
natural and/or recombinant proteins that
play a crucial role in cell signaling can be
assayed in a sandwich ELISA format. The

EDKs come with a straightforward and
easy-to-use instruction manual.
ELISA Development Kits for detection and quantification of human, mouse, and rat:
Interleukins
e.g. IL-1 alpha, IL-2, IL-4, IL-6, IL-7, IL-8, IL-12, IL-13, IL-17, IL-22, IL-33

Factors
e.g. CTGF, EGF, FGF-2, G-CSF, GM-CSF, beta-NGF, PDGF-BB, PLGF-1, VEGF
Chemokines
e.g. Eotaxin, IP-10, MCP-1, MCP-2, GRO-alpha, GRO-beta, MIP-1, RANTES,

SDF-1 alpha
We also supply EDKs for BD-1, BD-2,

BD-3, BMP-2, CNTF, IFN-gamma,
IGF-BP1, Leptin, NOV, PAI-1, Resistin,
RANKL, SCF, TNF-alpha, TPO and many

more interesting targets.
Please see our website for a complete and up-to-date list of our ELISA Development Kits.
Cytokines and Growth

Factors
Cytokines and chemokines are small proteins secreted by a
variety of cell types. They are important signal messengers
mediating cell communication and activating other target cells
through binding to specific receptors. Cytokines play a central
role in the immune system where they induce inflammatory
and immune responses or function as chemoattractants (chemokines). They also coordinate and regulate other biological
processes such as cell growth and development or tissue repair.
11
11
Cytokines and Growth

Factors
11.1 Cytokines, Growth Factors, Hormones

and soluble Receptors . . . . . . . . . . . . . . . . 179
11.2 Animal-free Cytokines and

Growth Factors . . . . . . . . . . . . . . . . . . . . 180
11.3 Cytokines and Growth Factors

for Stem Cell Research . . . . . . . . . . . . . . . 181
11 Cytokines and Growth Factors
179
11.1 Cytokines, Growth Factors, Hormones, and soluble Receptors
Cell-based bioassays
Functional studies
Human, mouse, and rat cell culture
supplements
Immunoassays
Features
High purity and biological activity
Increased stability
Produced in a range of different hosts
Economic bulk sizes are available on
request
PromKine offers an exceptional variety of
recombinant proteins or peptides of human, mouse or rat origin (>800 items!)
which function as signaling molecules in
intercellular communication and play important roles in both health and disease
states. They can initiate, regulate or otherwise affect different effector mecha-
nisms within the responding target cells

by binding to specific cell surface receptors and thus activating intracellular
signal transduction cascades. Other
cytokines mediate chemoattraction between cells (chemokines) and yet other
signaling proteins included in our wide
product range are essential growth factors and hormones which stimulate and
regulate diverse cellular processes such
as cell growth, proliferation, maturation,
and differentiation.
All cytokines, chemokines, growth factors, CD antigens, hormones, and soluble receptors available from PromoKine
meet the highest quality standards. They
are highly purified and biologically active and were produced in E. coli, yeast,
insect cells, plants, or mammalian cell
lines (to provide native posttranslational
modifications if required).
Purity is generally assessed by SDS-PAGE
and HPLC analysis and is in most cases
higher than 95%. Most of the prod-
ucts are delivered lyophilized (and are

very stable in that format), while some
come ready-to-use in a buffer solution.
Reconstitution of the lyophilized proteins
should be carried out in water or common buffer solutions.
Products available at PromoKine include human, mouse and rat Interleukins, Colony Stimulating Factors (CSFs),
Growth Factors, Interferons, Chemokines, Adipokines, Bone Morphogenetic
Proteins (BMPs), Fibroblast Growth Factors (FGFs), Matrix Metalloproteinases
(MMPs), CD antigens, Defensins, Tumor
Necrosis Factors (TNFs), soluble receptors, hormones, extracellular binding
proteins, non-inhibitory Serpins and
other immunoregulatory proteins.
We continuously add new recombinant
proteins to our inventory.
For an up-to-date product list please
visit www.cytokines.info
Interleukins
e.g. IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12,
IL-13, IL-15, IL-16, IL-17, IL-19, IL-20, IL-21, IL-22, IL-24, IL-31, IL-32, and IL-33
Growth and Differentiation Factors
e.g. BDNF, CTGF, CTGFL, EGF, EG-VEGF, FGFs (including FGF-1, FGF-2 and FGF-4),
GDFs, GDNF, HB-EGF, HDGF, HGF, IGF-1, IGF-2, IGF-BPs, KGF, beta-NGF, PDGFs (including PDGF-AA and PDGF-BB), PLGFs, TGF-betas (including TGF-beta 1), and VEGFs
(including VEGF-A)
Colony Stimulating Factors
e.g. G-CSF, GM-CSF, and M-CSF
Interferons
e.g. IFN-alpha, IFN-beta and IFN-gamma
Chemokines
e.g. BCA-1, BRAK, CTACK, CXCL16, ENA-78, Fractalkine, GCP-2, Eotaxin, Exodus, GROalpha, GRO-beta, GRO-gamma, HCC-1, IP-10, I-TAC, LEC, LIX, Lymphotactin, MCP-1,
MCP-2, MCP-3, MCP-4, MCP-5, MDC, MEC, MIG, MIP-1, MIP-3, MIP-4, NAP-2, PF-4,
RANTES, SDF-1 alpha, SDF-1 beta, TAFA, TARC, and TECK
Tumor Necrosis Factors
e.g. TNF-alpha and TNF-beta
Bone Morphogenetic Proteins
e.g. BMP-2, BMP-3, BMP-4, BMP-5, BMP-6, and BMP-7
Matrix Metalloproteinases
e.g. MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, and MMP-13
Hormones
e.g. Adiponectin, CNTF, EPO-alpha, FSH, GH, Ghrelin, Insulin, Leptin, Prolactin, Relaxin,
Resistin, and TPO
CD Antigens
e.g. CD1A, CD2, CD4, CD9, CD11b, CD14, CD22, CD23, CD25, CD29, CD31, CD34,
CD43, CD45, CD54, CD56, CD62, CD68, CD72, CD95, CD99, CD100, CD105, CD106,
CD120, CD124, CD137, CD141, CD146, CD147, CD152, CD154/CD40L, CD269, and
CD283
We also supply other important signaling proteins/peptides such as:

Activin A, APRIL, Angiogenin, Angiopoietin-1, Angiostatin, Apolipoproteins,
Artemin, BAFF, Betacellulin, Cardiotrophin-1, CRP, DKK-1, Endostatin, FGF
Receptors, flt-3 Ligand, Follistatin, Galectins, Granzyme B, Heregulin, ICAMs,

LIF, Maspin, Midkine, Myostatin, Nanog,
Neuregulin, Neuroglobin, Neuroserpin,
Neurotrophins, NOGGIN, Oncostatin M,
OPG, Osteonectin, Osteopontin, PAI-1
Please see www.cytokines.info for a complete and up-to-date list.
& PAI-2, RANKL, RELM-beta, SCF, Selectins, TNF Receptors, TIE-1 & TIE-2,
TIMP-1 & TIMP-2, TRAIL, VCAM-1,
VEGF Receptors, Vaspin, Vitronectin,
Visfatin, Wnt-3a and many more interesting targets.
Cytokines and
Growth Factors
Applications
180
11.2 Animal-free Cytokines and Growth Factors

Applications
Cell-based bioassays
Functional studies
Human, mouse, and rat cell culture
supplements
Immunoassays
Cytokines and
Growth Factors
Features
High purity and biological activity
Increased stability
Free of any impurities derived from
animals or bacteria
Economic bulk sizes are available on
request
PromoKines animal-free cytokines and
growth factors have been produced by
molecular farming using tobacco plants
or barley grain as the production hosts which have the G.R.A.S. (Generally Recognized As Safe) status from the FDA.
These cytokines are free of animal
components, serum, endotoxins, and
antibiotics, as well as human or animal
infectious agents or other endogenous
mammalian contaminants (e.g. traces
of unwanted growth factors and cytokines). As these proteins have been
expressed in plants, they show very low
proteolytic, pyrogenic, and inflammatory activity compared to other commercially available proteins such as those
produced in bacteria, yeast, insect, or
mammalian cells. In addition, they retain
proper eukaryotic folding and glycosylation resulting in an increased stability
and biological activity. Degradation due
to protease carryover as well as un-
wanted cell responses (e.g. proliferation

or inflammatory cascades) or cell growth
inhibition caused by contaminating serum components (serum carryover),
endotoxins or bacterial, viral, and prion
infectious agents are also eliminated or
at least extremely reduced.
PromoKines range of plant-derived
proteins were produced under strict
animal-free conditions. While avoiding
any potential variables and interfering effects associated with the use of
animal-derived manufacturing components they still maintain high biological activity and purity compared to the
corresponding proteins produced using
standard techniques. Thus, they are ideally suited for some very sensitive applications, e.g. in stem cell research.
Our range of animal-free cytokines and growth factors is continuously growing and includes:
Interleukins
e.g. IL-2, IL-3, IL-4, IL-6, IL-12

Factors
e.g. FGF-2, KGF, TGF-beta 2 and TGF-beta 3, and VEGF-165
Colony Stimulating Factors
e.g. GM-CSF, and M-CSF
Interferons
e.g. TNF-alpha
Bone Morphogenetic Proteins
e.g. BMP-2 and BMP-7
We also supply other important signaling proteins/peptides such as: Activin A,

Activin B, BAFF, flt-3 Ligand, Follistatin,
Growth Hormone, LIF, Myostatin,

sRANKL, SCF and many more interesting
targets that will come soon.
Please see www.promokine.info/AF for a complete and up-to-date list.
OD (490 nm)
1.6
1.5
Control fraction
PromoKines animal-free VEGF-121
1.4
1.3
1.2
0.1
Fig. 1: Cell proliferation assay

using PromoKines animal-free
VEGF-121 and HUVEC cells (see
page 13)
1.0
0.9
0.8
0.7
0.0
10-
10-
10-
1.0
10
ng/ml VEGF-121
10
10
181
Stem cells play a central role in the development of multicellular organisms

and diverse regeneration processes
throughout their lifes, e.g. replacing
diseased and damaged tissues. They are
unspecialized precursor cells characterized by their abilities of self-renewal
and pluripotency, that means they can
undergo numerous rounds of cell division maintaining the undifferentiated
state and are able to develop into different types of cells such as skin, muscle or
blood cells.
Mammalian stem cells are generally

comprised of two types: Embryonic stem
cells (ESCs) are derived from the epiblast
tissue of the inner cell mass of an early
stage embryo (blastocyst) and are considered pluripotent and capable to differentiate into any cell type of the adult
body. Adult stem cells (also known as
somatic stem cells) are undifferentiated
cells found in the tissues of children and
adults. Their main function is to replace
dead or damaged specialized cells and
to maintain the normal turnover of re-
generative organs. Most adult stem cells

are lineage-restricted (multipotent) and
characterized by a limited self-renewal
and differentiation capacity usually
restricted to cell types of the tissue in
which they reside. In contrast, induced
pluripotent stem cells (iPSCs) are not
adult stem cells but specialized somatic
adult cells (e.g. epithelial cells) that have
been genetically reprogrammed to pluripotent cells which are in many respects
similar to natural pluripotent stem cells
such as embryonic stem cells.
PromoKine provides a wide range of cytokines and growth factors (>300) which are important in stem cell research:
Mesenchymal Stem Cell Growth and Differentiation Factors
e.g. EG-VEGF, FGF-4, flt-3 Ligand, GDFs, GM-CSF, HGF, IFN-gamma, IL-6, SDF-1 alpha,
TGF-beta 2, and TGF-beta 3
Cytokines & Growth Factors for General

Propagation and Differentiation of Various
Adult Stem Cell Types
e.g. BAFF, BDNF, beta-NGF, BMP-2, BMP-4, BMP-7, EGF, FGF-2, flt-3 Ligand, G-CSF,
GM-CSF, IGF-1, IL-3, IL-6, IL-11, M-CSF, PDGF-AA, PDGF-BB, SCF, TARC, TGF-beta 1,
TPO, and VEGFs (e.g. VEGF-A and VEGF-C)
Cytokines for Propagation of Undifferentiated Pluripotent Human Embryonic Stem

Cells
e.g. Ang-1, LIF, Nanog, Wnt-1, and Wnt-3a
Hematopoietic Stem Cell Research
e.g. Ang-1, EPO-alpha, flt-3 Ligand, G-CSF, GM-CSF, Granzyme B, IFN-gamma, IGF-1,
IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, PDGF-AA, PDGF-BB, SCF, SDF-1 alpha,
TGF-alpha, TGF-betas (1-3), TNF-alpha, TPO, and VEGFs (e.g. VEGF-121 and VEGF165)
General Embryonic Stem Cell Research
e.g. Activin A, Activin B, Ang-1, BDNF, BMP-4, BMP-7, EGF, FGFs (e.g. FGF-2 and FGF-4),
GDF-3, Heregulin-beta, IGF-1, KGF, Noggin, Oncostatin M, TGF-alpha, TGF-beta 2, TGFbeta 3, and Vitronectin
Neural Stem Cell Research
e.g. FGF-2, EGF, Galectin-1, and Wnt-3a
Please see www.promokine.info/stemcells for a complete and up-to-date list.

Note: Find more products for stem cell research (Antibodies, Stem Cell Fate Regulators, etc.) on our website.
Cytokines and
Growth Factors
11.3 Cytokines and Growth Factors for Stem Cell Research
Microbial Detection
and Elimination
Cell culture contamination is a common problem that impacts
many biochemical and immunological properties of cells and it
leads to unsatisfactory and unreliable results. Therefore, regular
testing of cell cultures for mycoplasmas/bacteria contamination
is essential.
PromoKine provides highly sensitive and user-friendly test kits
for this purpose, as well as products for the effective elimination
and prevention of mycoplasma and eubacteria contamination.
12
12
Microbial Detection
and Elimination
12.1 Mycoplasma Test Kits . . . . . . . . . . . . . . . 185
12.2 Mycoplasma Elimination Solutions . . . . . . 186
12.2.1 Mycoplasma-Ex . . . . . . . . . . . . . . . . . . . . . 187
12.2.2 BIOMYC Antibiotic Solutions . . . . . . . . . . . 187
12.3 Bacteria Test Kit . . . . . . . . . . . . . . . . . . . . 188

12.4 Disinfectants . . . . . . . . . . . . . . . . . . . . . . . 189
12.4.1 Mycoplasma-Ex Spray . . . . . . . . . . . . . . . . 189
12.4.2 Pharmacidal Spray . . . . . . . . . . . . . . . . . . . 189
12.4.3 Aquaguard . . . . . . . . . . . . . . . . . . . . . . . . . 190
12.4.4 Mycoplasma-EX Wipes . . . . . . . . . . . . . . . . 190
12 Microbial Detection and Elimination
185
12.1 Mycoplasma Test Kits
Mycoplasma
detection in cell culture,

virus stocks, and medium
Conventional and real-time PCR
Features
Highly
sensitive (detects 10 - 15
mycoplasmas per sample volume)
Fast, reproducible, and reliable results
Applicable for all cell/tissue culture relevant mycoplasma species
Positive and internal controls included
Convenient protocol and easy sample
preparation
Mycoplasmas are parasitic bacteria that
frequently contaminate tissue cultures
and consequently also virus stocks, vaccines, and other biological materials produced in cells. The contamination can
originate from serum, laboratory staff,
other contaminated cultures, or the donors from which the cells have been harvested, and it often remains unnoticed.
Although mycoplasmas do not cause
visible cell damage, they seriously affect
cell growth and metabolism, transfection efficiency, protein and monoclonal
antibody synthesis, cytokine secretion,
immunological properties, signal transduction, virus proliferation and can even
cause DNA and RNA damage.
Since mycoplasmas are very small and
lack cell walls, detection using a conventional light microscope is nearly impossible. In addition, cloudiness in the
medium, typical for bacterial or yeast

contaminations, does not occur even at
high mycoplasma concentrations. PCR
on the other hand is a highly sensitive,
specific, and rapid method for the detection of mycoplasma contamination
in cell culture supernatant, cell/virus
stocks, and medium. All cell/tissue culture relevant mycoplasma species can
be detected in one assay including M.
fermentans, M. hyorhinis, M. arginini,
M. orale, M. salivarium, M. hominis,
M. pulmonis, and M. pirum. In addition, Acholeplasma laidlawii, M. synoviae, and Ureaplasma species can be
detected.
PCR Mycoplasma Test Kit I/C

The PCR Mycoplasma Test Kit I/C utilizes conventional PCR technology for
convenient and specific detection of all
known cell culture-contaminating mycoplasma species. The primer set is specific for the highly conserved 16S-rRNA
coding region in the mycoplasma
genome. The test can be performed in 3
hours and includes internal control DNA
(for verifying a successful PCR run) as
well as positive control DNA. All components required for the PCR (primers,
nucleotides, internal control DNA) come
optimally premixed and lyophilized in
PCR tubes (except the Taq polymerase).
For setting up the PCR, simply cut off
the number of tubes required, rehydrate
with rehydration buffer (containing the
Hot-Start Taq polymerase), and add the
sample to be analyzed. Samples can be

easily prepared by boiling the cell culture
supernatant or other sample material.
PCR Mycoplasma Test Kit I/RT

The PCR Mycoplasma Test Kit I/RT utilizes Real-Time PCR technology for convenient and reproducible detection of
contaminating mycoplasmas. It shows
excellent sensitivity and specificity and
enables reliable, quantitative PCR results quickly (making the kit particularly
useful for routine testing). The primer
and probe set is specific for the highly
conserved 16S-rRNA coding region in
the mycoplasma genome. It contains
FRET probes emitting fluorescent light
at 520 nm and is compatible with most
common Real-Time PCR cyclers (e.g.
Roche Light Cycler 1 and 2, ABI Prism,
Cepheid Smart Cycler, and Eppendorf
Mastercycler ep realplex).
The test includes internal control DNA
(for verifying a successful PCR run) as
well as positive control DNA. All components required for Real-Time PCR
(primers, probes, nucleotides, and internal control DNA) come lyophilized
in PCR tubes, except the Hot-Start Taq
polymerase which is dissolved in reaction buffer. Two kit variants (kit A for
e.g. Light Cycler, kit B for e.g. ABI Prism)
are provided which have been optimized
for different Real-Time PCR instruments.
For recommended kit variant, please see
www.promokine.info where you can
download the manual.
PCR
100 l cell culture
supernatant
2 l supernatant
Analyze 5 l of
the PCR sample
Boil for 10 minutes

and centrifuge briefly
Fig. 1: Mycoplasma detection with the PromoKine PCR Mycoplasma Test Kit I/C is easy and convenient: Boil 100 l cell culture
supernatant or other sample material for 10 minutes; centrifuge briefly and add 2 l supernatant to the reaction cup with the
rehydrated PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis. When
using the PCR Mycoplasma Test Kit II, the sample is directly added to the ready-to-use, complete reaction mix to set up the PCR.
Microbial Detection
and Elimination
Applications
186
PCR Mycoplasma Test Kit II
The kit includes all components required

for PCR (nucleotides, primers, a highperformance Taq polymerase, magnesium and an agarose gel loading dye)
premixed at optimized concentrations
in a ready-to-use master mix. The test
takes approximately five hours to obtain clear results and reliably detects all
Microbial Detection
and Elimination
The PCR Mycoplasma Test Kit II utilizes conventional PCR technology for
convenient and reliable detection of
mycoplasmas. The primer set is specific
for the highly conserved 16S-rRNA coding region in the mycoplasma genome.
mycoplasma species commonly found in

contaminated cell cultures.
The kit also contains positive control
DNA for easy result verification but, different to the PCR Mycoplasma Test Kit
I/C, contains no internal control DNA.
Fig. 2: Detection of mycoplasmas with

PCR Mycoplasma Test Kit I/C.
M: 100 bp DNA ladder
1: Positive control
2-6: 10; 100; 1,000; 10,000; 100,000
mycoplasmas/sample volume
Internal control DNA: 479 bp band
Positive sample/control: 270 bp band
479 bp
270 bp
Product
Size
Catalog Number
24 tests
48 tests
96 tests
PK-CA91-1024
PK-CA91-1048
PK-CA91-1096
PCR Mycoplasma Test Kit I/RT; Variant A
25 tests
PK-CA91-3025A
PCR Mycoplasma Test Kit I/RT; Variant B
25 tests
PK-CA91-3025B
PCR Mycoplasma Test Kit II
20 tests
PK-CA20-700-20
Mycoplasma fermentans DNA Standard

(for qPCR)
100 l
PK-CA91-0117
Mycoplasma orale DNA Standard (for

qPCR)
100 l
PK-CA91-0112
RTU DNA Ladder I (100 bp)
0.5 ml
PK-MB713-001N
RTU DNA Ladder II (1000 bp)
0.5 ml
PK-MB713-002N
Please see page 125 for the ready-to-use DNA Ladders.
12.2 Mycoplasma Elimination Solutions

Mycoplasma contamination seriously affects cell growth and cell physiology leading to biased and unreliable experimental
results. When mycoplasma contamination is detected, the contaminated cells
are ideally discarded and all equipment,
e.g. work benches, CO2 incubators, etc.,
are disinfected (see page 189 for disinfectant sprays). However, if the affected cells are unique or the result of
extensive work, thorough elimination of
the contaminating mycoplasmas is necessary. Standard antibiotics commonly
used in cell culture (e.g. Pen/Strep) do
not affect mycoplasmas. Therefore,

PromoKine offers two highly effective
methods to get rid of mycoplasma contamination: Mycoplasma-EX, a combination of non-antibiotic and antibiotic
reagents, and BIOMYC 1-3, three highly
effective antibiotics.
187
12.2.1 Mycoplasma-EX
Mycoplasma
elimination in cell/tissue
culture
Mycoplasma elimination in virus stocks
Features
Highly
effective against mycoplasma

species commonly found in cell
cultures
Very short treatment time
Very low cytotoxicity
Ready to use
Easy handling
Mycoplasma-EX combines a non-antibiotic and an optimized antibiotic treat-
ment for the effective elimination of mycoplasmas and related microorganisms

from cell/tissue culture and virus stocks.
It eliminates mycoplasmas by directly
killing them and not just by inhibiting
their growth, with minimal toxicity for
the cells in culture.
Mycoplasma-EX is effective after a single
treatment and destroys mycoplasmas
rapidly and permanently. It is applicable
for most cell lines (e.g. Vero, BHK21,
GBK, ML, Hep2, 293, CRFK, H9, Molt4,
MT-4, Jurkat), primary cells as well as
virus stocks (e.g. SHV-1, BHV-1, HSV-1,
VSV, SFV, FCV, MEV) and is highly effective against M. orale, M. hyorhinis,
M. hominis, Acholeplasma laidlawii,
M. arginini, M. fermentans, and other
mycoplasma species encountered as

contaminants in cell cultures.
Fig. 3: Fluorescence microscope image of

cells contaminated with mycoplasmas.
Picture kindly provided by M. Ebert,
Forschungszentrum fr Medizintechnik
und Biotechnologie, Erfurt, Germany.
12.2.2 BIOMYC Antibiotic Solutions

Applications
Mycoplasma
elimination in cell/
tissue culture
In addition, they frequently just reduce

the concentration of mycoplasmas rather
than completely eliminating them. As
soon as treatment is discontinued, contamination will recur.
Features
BIOMYC-1 and 2
Highly
effective against mycoplasma

species commonly found in cell
cultures
Low cytotoxicity
Ready to use
Easy handling
Antibiotics that impact bacterial cell walls
(e.g. penicillin) are ineffective against
mycoplasmas which lack a cell wall.
Other antibiotics such as tylosin, neomycin, tetracycline, or gentamicin are
only effective against a few mycoplasma
species commonly found in cell cultures.
BIOMYC-1 and 2 are two antibiotics

which eliminate mycoplasmas very efficiently when used in combination. After
a four-day treatment with BIOMYC-1,
a three-day treatment with BIOMYC-2
follows. This is repeated 2 to 3 times.
Following this protocol, mycoplasmas
will not develop resistance to these antibiotics - a common occurrence with
other antibiotic treatment methods.
BIOMYC-1 is based on the antibiotic tiamutin which is produced by the fungus
Pleurotus mutilus. BIOMYC-2 is based
on the antibiotic minocycline, an improved derivative of tetracycline.
BIOMYC-3
BIOMYC-3 is based on the antibiotic
ciprofloxacin which is a member of the
fluoroquinolone group and inhibits
synthesis of DNA gyrase, an enzyme
necessary for bacterial DNA supercoiling. Many mycoplasma species are
sensitive to BIOMYC-3, including M.
orale, M. hyorhinis, M. fermentans, and
M. arginini, and also Acheloplasma laidlawii. These species are responsible for
most mycoplasma contaminations in cell
cultures. No cytotoxic effects on the cells
in culture have been found at the concentrations recommended for use, and
the treatment can be performed within
12 days.
Product
Size
Catalog Number
3 treatments
PK-CC91-4003
BIOMYC-1
10 ml
20 ml
100 ml
PK-CC03-036-1D
PK-CC03-036-1C
PK-CC03-036-1B
BIOMYC-2
10 ml
20 ml
100 ml
PK-CC03-037-1D
PK-CC03-037-1C
PK-CC03-037-1B
BIOMYC-3
10 ml
20 ml
100 ml
PK-CC03-038-1D
PK-CC03-038-1C
PK-CC03-038-1B
Mycoplasma-EX
Microbial Detection
and Elimination
Applications
188
12.3 Bacteria Test Kit

Applications
Bacteria detection in cell culture, virus
stocks, and medium
Features
Highly
Microbial Detection
and Elimination
sensitive (detects 12 bacteria

genomes per sample volume)
Reproducible and reliable results
Applicable for 45 bacteria species
Positive and internal controls included
Convenient protocol and easy sample
preparation
cobacterium, Peptostreptococcus, Pseudomonas, Porphyromonas, Prevotella,

Salmonella, Serratia, Staphylococcus,
Streptococcus, etc.). The kit provides a
highly sensitive, easy, and rapid method
to detect bacterial infection in various biological materials, including cell cultures.
Detection requires as little as 12 bacteria
genomes (~52 fg) per sample volume.
The protocol can be completed within
3.5 hours with a hands-on time of less
than 40 minutes.
decoloration of the medium. In addition,

it has been demonstrated that standard
antibiotics are mostly ineffective against
resistant bacterial infection but have
a strong impact on the metabolism,
growth, and differentiation of the cells
in culture.
Bacteria are frequently found as contaminants in cell cultures. However, cell

cultures sometimes lack visual signs of
the bacterial contamination, such as fast
Frequent screening of cell cultures for

bacterial contamination is essential
to ensure reliable results. Therefore,
PromoKine offers a PCR-based Bacteria
Test Kit that has been developed for
accurate and reliable in vitro diagnosis of
45 bacteria species which are the common contaminants in cell cultures (e.g.
Actinomyces, Bacillus, Enterococcus,
Escherichia coli, Fusobacterium, Klebsiella, Lactobacillus, Micrococcus, My-
The kit contains all the necessary components for setting up a PCR - including a
positive control DNA as well as an internal control DNA to confirm a successfully
performed PCR run. The kit does not
amplify eukaryotic DNA.
PCR
200 - 500 l cell
culture supernatant
Analyze 5 l of
the PCR sample
5 l DNA sample
Extract Bacterial
DNA
Fig. 4: Bacteria detection with the PromoKine PCR Bacteria Test Kit is easy and convenient: Purify bacterial DNA from 200 500 l
of cell culture supernatant using an appropriate DNA extraction kit; add 5 l sample DNA to the reaction cup with the premixed
PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis.
Product
Size
Catalog Number
PCR Bacteria Test Kit
24 tests
PK-CA91-2024
48 tests
PK-CA91-2048
PromoKine also offers a Bacteria Live/Dead Staining Kit (see page 105) as well as fluorescent dyes for staining of bacteria (see
page 107).
189
12.4 Disinfectants
Disinfection of all tissue culture equipment and work surfaces in the lab has
to be performed regularly to prevent
microbial contamination. PromoKine of-
fers different disinfectants ideal for laboratory surfaces and apparatuses, CO2
incubators, and water baths.

www.promokine.info/knowledge-base
Mycoplasma-EX Spray allows safe disinfection and cleaning of all laboratory surfaces and apparatuses, including work
benches, incubators, cell storage boxes,
and liquid nitrogen containers. It utilizes
the powerful Mycoplasma-EX reagent in
combination with an alcohol-based solution. Besides its strong mycoplasmacidal
activity, it is very effective e.g. against
Staphylococcus aureus, Streptococcus
faecalis, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Candida
albicans, Mycobacteria, HBV/HIV, Papova, Adeno, Polio and Vaccinia viruses.

The effectiveness of Mycoplasma-EX
Spray is further enhanced by its excellent
detergent properties, achieving clean and
contamination-free surfaces in one step.
Applications
Disinfection
Disinfection
of all laboratory surfaces

of laboratory apparatuses
Features
Kills
mycoplasmas within seconds

against most microorganisms
Non-corrosive and non-cancerogenic
Does not leave any residue
Supplied in a refillable spray bottle
Effective
Product
Mycoplasma-EX Spray
Mycoplasma Disinfection Kit I
(Consists of Mycoplasma-Ex Spray 500 ml,
Aquaguard-1, and Aquaguard-2)
Size
Catalog Number
500 ml
5L
PK-CC91-5050
PK-CC91-5050-5L
1 Kit
PK-CC91-5060
12.4.2 Pharmacidal Spray

Applications
Non-corrosive
and non-cancerogenic
not leave any residue
Supplied in a refillable spray bottle
Does
Disinfection
of incubators
Features
Free
of mercury, formaldehyde,
phenol, and alcohol
Very effective against bacteria, fungi,
spores, and viruses
Pharmacidal Spray is a mercury-, formaldehyde-, phenol-, and alcohol-free

disinfectant specifically designed for the
disinfection of incubators. Spraying the
incubators thoroughly every two weeks
is recommended.
Product
Pharmacidal Spray
Mycoplasma Disinfection Kit II
(Consists of Pharmacidal Spray 1 L,
Size
Catalog Number
1000 ml
5L
PK-CC-110100
PK-CC-110100-5L
1 Kit
PK-CC91-5070
Microbial Detection
and Elimination
12.4.1 Mycoplasma-EX Spray
190
12.4.3 Aquaguard
Applications
Disinfection
of water trays in CO2

incubators
Disinfection of water baths
Microbial Detection
and Elimination
Features
Very
effective against bacteria, fungi,

spores, and viruses
Non-corrosive
Non-toxic and non-volatile
The water in an incubator or water bath

is a major source of cell culture contamination, particularly with respect
to mycoplasma contaminations, since
mycoplasmas can survive in pure water.
Aquaguard, a non-volatile disinfectant,
is extremely effective at eliminating the
microorganisms in water. In addition, it
does not cause damage to the stainless
steel of the incubator or any plastic interior and it does not cause any skin irritation when used as recommended.
Aquaguard-1 is intended for disinfecting water in incubators, whereas Aquaguard-2 is intended for water bath disinfection.
Both solutions keep the water reliably
free of undesired microorganisms.
Product
Size
Catalog Number
100 ml
10 x 100 ml
PK-CC01-867-1B
PK-CC01-867-10B
50 ml
10 x 50 ml
PK-CC01-916-1E
PK-CC01-916-10E

(Consists of Mycoplasma-Ex Spray 500 ml,
1 Kit
PK-CC91-5060

(Consists of Pharmacidal Spray 1 L,
1 Kit
PK-CC91-5070
Aquaguard-1 (incubator)
Aquaguard-2 (water bath)
12.4.4 Mycoplasma-EX Wipes

Mycoplasma-EX Wipes are an effortless
way to clean and disinfect a variety of
laboratory surfaces such as work benches, pipettors, cell storage boxes, the
incubator interior, etc. There is no need
for multiple products when you have the
convenience of a simple-to-use wipe.
Just wipe, toss and youre done!
Similar to our Mycoplasma-EX Spray,
Mycoplasma-Ex Wipes work efficiently
against mycoplasmas, fungi, bacteria,

viruses and prevent any microbial contamination. Mycoplasma-Ex Wipes contain an alcohol-based, non-corrosive and
non-carcinogenic solution with a reagent
that kills mycoplasmas effectively and
fast.
Mycoplasma-EX Wipes are excellent for
maintaining a clean, contamination-free
work area and also allow fast and easy
cleaning and disinfection of alcoholresistant surfaces. They can be used

where it is not possible to spray and
where rapid drying is important.
Mycoplasma-EX Wipes are supplied as
single-use wipes in a convenient product
safety container. Economic refill sachets
are also available.
Product
Mycoplasma-EX Wipes
Size
Catalog Number
120 pieces
5 x 120 pieces
PK-CC91-5055
PK-CC91-5055-5
Alphabetical Index
191
Description
Page
1-9
Description
Page
Annexin V-Biotin Detection Kit
133
Annexin V-Biotin Reagent
133
4-Bromo A-23187 free acid
118
132
5(6)-CFDA
117
Annexin V-Cy3 Detection Kit Plus
132
5(6)-CFDA, SE (CFSE)
117
133
5-(and-6)-Carboxy-2',7'-dichlorofluorescein
117
132
5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate
117
133
5-(and-6)-Carboxyfluorescein
117
Annexin V-EGFP Detection Kit
132
5,5'-dibromo-Bapta (tetrapotassium salt)
116
Annexin V-EGFP Reagent
133
5,5'-difluoro-Bapta (tetrapotassium salt)
116
Annexin V-FITC Detection Kit
132
5,5'-difluoro-Bapta AM
116
Annexin V-FITC Detection Kit Plus
132
5,5'-dimethyl BAPTA, AM ester
116
Annexin V-FITC Reagent
133
5,5'-dimethyl BAPTA, tetrapotassium salt
116
Annexin V-PE Detection Kit
133
5-Carboxy-2',7'-dichlorofluorescein
117
Annexin V-PE Detection Kit Plus
133
5-Carboxy-2',7'-dichlorosulfonefluorescein
117
Annexin V-PE Reagent
133
5-CFDA
117
Annexin V-PE-Cy5 Detection Kit
133
5-FAM
117
Annexin V-PE-Cy5 Reagent
133
6-CFDA
117
Antibiotics and Antimycotics
97
6-FAM
118
Antibodies
169
7-AAD (7-aminoactinomycin D)
109
Antigens
172
Aortic Adventitial Fibroblasts
28
Aortic Endothelial Cells
15
Aortic Smooth Muscle Cells
37
A
A-23187 free acid
118
Apoptosis
128
Accutase-Solution
95
Apoptosis antibodies
169
Acridine Orange (AO) Solution
109
Apoptosis ELISAs
176
Actinomycin D
141
Apoptosis Inducer Set
141
Actinomycin D (10 mM)
141
Apoptotic Cell Isolation Kit
141
Adipocyte Media
77
129
Adipogenic Differentiation Medium
80
Apoptotic DNA Ladder Detection Kit I plus
129
ADP Fluorometric & Colorimetric Quantitation Kit
121
Apoptotic DNA Ladder Detection Kit II
129
ADVASEP-7 (sulfobutyl b-cyclodextrin)
112
Apoptotic/Necrotic Cells Detection Kit
140
Airway Epithelial Cell Media
65
Apoptotic/Necrotic/Healthy Cells Detection Kit
140
AK Cytotoxicity Kit
106
Aquaguard-1 (incubator)
190
Akt Activated Cell Lysate
139
Aquaguard-2 (water bath)
190
139
ATP Fluorometric & Colorimetric Quantitation Kit
121
Akt Inhibitor
139
Akt Negative Control Cell Lysate
139
Allophycocyanin
152
Alpha-Bungarotoxin (from Bungarus multicinctus)
112
105
AM1-43
112
Bapta (tetracesium salt)
116
AM1-44
112
Bapta (tetrapotassium salt)
116
AM2-10
112
Bapta (tetrasodium salt)
116
AM3-25
111, 112
Bapta AM
116
AM4-64
112
Bapta-FF (free acid)
116
AM4-66
112
Bapta-FF AM
116
Amino Acid Solutions
99
BCECF
110, 118
Amphotericin B Solution
97
BCECF AM
110, 118
Anisomycin
141
BCECF AM Solution
110, 118
Annexin V Binding Buffer
133
beta-Galactosidase Reporter Assay Kit
108
Annexin V Unlabeled Reagent
133
beta-Galactosidase Staining Kit
108
Appendix
Alphabetical Index
Appendix
192
Alphabetical Index
Description
Page
Description
Page
Beta-Secretase Fluorometric Assay Kit
119
Cardiac Fibroblasts
28
Beta-Secretase Inhibitor I
119
Cardiac Microvascular Endothelial Cells
20
Beta-Secretase Inhibitor II
119
Cardiac Myocytes
11
Betulinic acid
141
Caspase Inhibitors
136
Biocytin (e-biotinoylL-lysine)
113
Caspase Screening Kit
135
Biocytin-Hydrazide
113
Caspase Substrates
136
Bioluminescent Cell Viability Kit I (ATP)
104
137
Bioluminescent Cell Viability Kit II (ADP/ATP)
104
135
BIOMYC-1
187
137
BIOMYC-2
187
137
BIOMYC-3
187
135
Biotin PCR Labeling Kit
149
137
Biotin-4-Fluorescein
113
137
Biotin-AM3-25
112
137
Biotin-dATP (PCR)
151
135
Biotin-dCTP (PCR)
151
137
Biotin-DHPE
111
137
Biotin-dUTP (PCR)
151
135
Biotin-Ethylenediamine, hydrobromide
113
137
Biotin-Ethylenediamine, hydrochloride
113
137
Biotin-Rhodamine 110
113
137
Biotin-X-DHPE
111
137
Biotin-XX-a-Bungarotoxin
112
135
Bis-Fura-2, hexapotassium salt
116
137
Bladder Microvascular Endothelial Cells
20
137
Bladder Smooth Muscle Cells
40
135
Blood Cell Separation Medium
84
137
Blood Cells
48
137
Blue-fluorescent Cytoplasmic Membrane Staining Kit
111
135
99
137
B-Phycoerythrin
152
135
Brain Microvascular Endothelial Cells
22
137
Brefeldin A
141
137
Bronchial Epithelial Cells
24
135
Bronchial Smooth Muscle Cells
40
Caspase-8 Fluorometric & Colorimetric Assay Kit
137
Buffers and Salt Solutions
98
137
137
137
135
C
CaCl2-Solution
98
137
Calcein AM
110, 114
Caspases, active
136
Calcein AM Solution
110, 114
Cathepsin B Activity Assay Kit
138
110, 114
Cathepsin B&L Inhibitor Z-Phe-Phe-FMK
138
Calcium Calibration Buffer Kit
118
Cathepsin B, human (native)
138
Calpain 1, human (native)
138
Cathepsin D Activity Assay Kit
138
Calpain 1 Inhibitor Screening Kit
138
Cathepsin D Inhibitor Screening Kit
138
Calpain Activity Assay Kit
138
Cathepsin D, human (native)
138
Calpain Inhibitor Z-LLY-FMK
138
Cathepsin G Activity Assay Kit
138
cAMP Activity Assay Kit
119
Cathepsin H Activity Assay Kit
138
Camptothecin
141
Cathepsin H, human (native)
138
Camptothecin (2 mM)
142
Cathepsin K Activity Assay Kit
138
Description
Page
Description
Page
Cathepsin L Activity Assay Kit
138
Colorimetric Cell Viability Kit III (XTT)
104
Cathepsin L, human, recombinant
138
Colorimetric Cell Viability Kit IV (MTT)
104
Cathepsin S Activity Assay Kit
138
Colorimetric DNA Fragmentation Detection Kit (IHC)
129
Cathepsin S, human (native)
138
Colorimetric Glutathione Detection Kit
131
CD Antigens, recombinant
179
Colorimetric Glutathione Reductase Activity Kit
131
CD133 Progenitor Cells
53
Colorimetric GST Assay Kit
121
CD14+ Monocytes
54
Colorimetric Nitric Oxide Assay Kit
121
CD34+ Progenitor Cells
52
122
96
Coronary Artery Endothelial Cells
15
Cell Fractionation
122
Coronary Artery Smooth Muscle Cells
38
Cell Lysates
172
Cryo-SFM
79
Cell Pellets
91
Cycloheximide
142
104
Cycloheximide (100 mM)
142
Cell Transfection
156
Cytochrome c Apoptosis Detection Kit
130
CellClean
165
Cytokine antibodies
169
CFSE; 5(6)-CFDA, SE
110
Cytokine ELISAs
175
cGMP Activity Assay Kit
119
81
Chemokines antibodies
169
178
Chemokines ELISAs
175
Cytokines, recombinant
179
Chemokines, recombinant
179
122
Chk2 Inhibitor
43
Cytotoxicity Kits
106
Chondrocyte Media
62
Chondrocytes
12
Chondrogenic Differentiation Medium
80
Chromogenic beta-Galactosidase Substrate Sampler Kit
108
DAPI
109
c-Jun (1-79)-GST Fusion Protein
139
DAPI Solution
109
Classical Media
85
DAPI, dilactate
109
DMEM Media
85
DASPEI
109
DMEM/Hams F-12 Media
85
Daunorubicin.HCl
142
Hams F-10 Media
85
DC Base Media
82
Hams F-12 Media
85
DC Generation Media
82
Leibovitz L-15 Medium
85
Dendritic Cell Media
82
McCoys 5A Media
86
Dermal Blood Endothelial Cells
18
MEM Media
86
Dermal Fibroblasts
27
RPMI 1640 Media
86
Dermal Lymphatic Endothelial Cells
19
Cloning Vectors
166
Dermal Microvascular Endothelial Cells
17
108, 116
DetachKit 1/2
95
Coelenterazine 400a
108, 116
Detachment Reagents
95
Coelenterazine cp
108, 116
Dexamethasone
142
Coelenterazine f
108, 116
Dexamethasone (10 mM)
142
Coelenterazine fcp
108, 116
Di-12-ANEPPQ
113
Coelenterazine h
108, 117
Di-2-ANEPEQ (JPW1114)
113
Coelenterazine hcp
108, 116
Di-4-ANEPPS
113
Coelenterazine i
108, 117
Di-8-ANEPPQ
113
Coelenterazine ip
108, 116
Di-8-ANEPPS
113
Coelenterazine n
108, 117
DiA
112
108, 117
DiB
112
108, 117
DiBAC4(3)
113
Colchicine
142
DiD (DiIC18[5])
111
Colorimetric Cell Viability Kit I (WST-8)
104
Dihydrorhodamine 123
109
Colorimetric Cell Viability Kit II (WST-1)
104
Dihydrorhodamine 123, dihydrochloride salt
109
193
Appendix
Alphabetical Index
Appendix
194
Alphabetical Index
Description
Page
Description
Page
DiI (or DiIC18[3])
111
88
DiI in vegetable oil
111
109, 110
DiIC1(5)
113
109, 110
Dilinoleyl DiI
111
Etoposide
142
Dilinoleyl DiO
111
Etoposide (10 mM)
142
DiO (or DiOC18[3])
111
Expression Vectors
166
DiO/DPA Membrane Potential Detection Kit
113
Extracellular Matrix Proteins
99
DiOC14(3), hydroxyethanesulfonate
111
DiOC16(3)
111
DiOC2(3)
113
DiOC5(3)
113
Fetal Calf Serum
87
DiOC6(3)
109, 110, 113
FFP-18 AM
116
DiR (DiIC18[7])
111
Fibroblast Media
70
Disinfectants
189
Fibroblasts
27
D-Luciferin, Free Acid
108
Fibronectin Solutions
99
D-Luciferin, Potassium Salt
108
FIP-18 AM
116
D-Luciferin, Sodium Salt
108
Flubi-2
118
DMAO
109
Flubida-2
118
DMEM Media
85
Fluo-3 (pentaammonium salt)
115
DMNPE-caged D-Luciferin
108
Fluo-3 (pentapotassium salt)
115
DMSO, anhydrous
114, 118
Fluo-3 (pentasodium salt)
115
121
Fluo-3 AM
115
DNA Gel Stains
125
Fluo-3 AM Solution
115
DNA Isolation
124
FluoDNA Gel Stain
125
DNA Ladder I (100 bp)
125
123
DNA Ladder II (1000 bp)
125
Fluorescein-a-bungarotoxin
112
DNA Size Markers
125
Fluorescein-Biotin
114
Doxorubicin.HCl
142
Fluorescein-DHPE
111
DPA
112
144
DPA/Terbium for membrane fusion assay
114
Fluorometric Cell Viability Kit I (Resazurin)
104
DPX
114
Fluorometric Cell Viability Kit II (Calcein)
104
Dulbeccos PBS
98
Fluorometric DNA Fragmentation Detection Kit I (BrdU)
129
Fluorometric DNA Fragmentation Detection Kit II (BrdU)
129
Fluorometric DNA Fragmentation Detection Kit III (F-dUTP)
129
Fluorometric Glutathione Detection Kit
131
EB (ethidium bromide) Solution
109, 110
Fluorometric GST Assay Kit
121
ECL Chemiluminescence Detection Kit
174
Fluorometric Nitric Oxide Assay Kit
121
ELISA Development Kits
176
Follicle Dermal Papilla Cell Media
72
ELISAs
175
30
99
Freezing Medium
79
Endothelial Cell Growth Supplement/Heparin
99
Fura-2 (pentaammonium salt)
115
Endothelial Cell Media
63
Fura-2 (pentapotassium salt)
115
Endothelial Cell Media MV
64
Fura-2 (pentasodium salt)
115
Endothelial Cells (Large Vessels)
13
Fura-2 AM
115
Endothelial Cells (Microvascular)
17
Fura-2 AM Solution
115
82
Fura-2FF (K Salt)
116
Epidermal Keratinocytes
32
Fura-2FF AM
116
Epidermal Melanocytes
33
Furaptra, AM ester
116
Epithelial Cell Media
65
Furaptra, tetrapotassium salt
116
Epithelial Cells
23
Furaptra, tetrasodium salt
116
Description
Page
Description
Page
HCMEC
20
HDBEC
18
G-418 Sulphate Solution
97
HDLEC
19
GelQuick Protein Gel Stain/Destaining Kit
123
HDMEC
17
Gene Cloning and Expression
166
Hematopoietic Progenitor Cell Expansion Medium DXF
81
GeneBooster Reagent I
165
Hematopoietic Progenitor Cell Media
81
GeneBooster Reagent II
165
Hepatocyte Media
73
GeneBooster Reagent III
165
Hepatocytes
31
GeneBooster Reagent Kit
165
HEPES Buffer
98
Gentamicin Solution
97
HEPES Buffered Saline Solution
98
Glutamine, L
99
HFDPC
30
Glutamine, Stable
99
HHpC
31
134
HMEpC
25
134
hMNC-CB
57
134
hMNC-PB
56
134
hMoCD14+-PB
54
134
hMo-PB
54
Green Multi-Caspase Staining Kit
134
hMSC-AT
49
Green-beta-D-Gal
108
hMSC-BM
49
Green-fluorescent Cytoplasmic Membrane Staining Kit
111
hMSC-UC
49
Growth Factor antibodies
169
HNEpC
23
Growth Factor ELISAs
175
HOB
34
Growth Factors, recombinant
179
Hoechst 33258
109, 110
Growth Supplements
99
109, 110
GSK-3a-GST Fusion Protein
139
Hoechst 33342
109, 110
GSK-3b, human, recombinant
139
109, 110
GST Inhibitor-2 (ethacrynic acid)
121
Hormone ELISAs
175
GST protein (active)
121
Hormones, recombinant
179
HPAEC
16
HPASMC
38
hPC-PL
51
H
Hams F-10 Media
85
HPF
28
Hams F-12 Media
85
HPlEpC
26
Hanks BSS
98
HPMEC
21
HAoAF
28
HPrSMC
41
HAoEC
15
HRCEpC
26
HAoSMC
37
HREpC
25
121
HSAEpC
24
HAT protein (P/CAF, active)
121
HSaVEC
16
HBdMEC
20
HTEpC
23
HBdSMC
40
HTSMC
39
HBEpC
24
HUAEC
14
HBMEC
22
HUASMC
39
HBSMC
40
HUF
29
HCAEC
15
Human Insulin
99
HCASMC
38
Human Primary Cells
10
hCD133+-CB
53
Cardiac Myocytes
11
hCD34+-CB
52
Chondrocytes
12
HCF
28
Endothelial Cells (Large Vessels)
13
HCH
12
Endothelial Cells (Microvascular)
17
HCM
11
Epithelial Cells
23
195
Appendix
Alphabetical Index
196
Alphabetical Index
Appendix
Description
Page
Description
Page
Fibroblasts
27
Luciferase Reporter Assay Kit I (Firefly)
107
30
Luciferase Reporter Assay Kit II (Renilla)
107
Hepatocytes
31
Luciferase Reporter Assay Kit III (Firefly & Renilla)
107
Keratinocytes
32
Luciferase Reporter HTS Kit (Firefly)
107
Melanocytes
33
Lymphatic Endothelial Cells
19
Osteoblasts
34
Lymphocyte Separation Medium 1077
84
Preadipocytes
35
36
Smooth Muscle Cells
37
48
83
Human Transferrin
99
83
HUtMEC
21
164
HUtSMC
41
Macrophage Base Medium DXF
83
HUVEC
13
96
HVMF
29
Macrophage Generation Media DXF
83
HWP
35
Magnet Plate
164
Hydroxystilbamidine
114
Magnetic Separator (re-usable)
141
Hygromycin B Solution
97
Mammalian Whole Cell Protein Extraction Kit
122
Mammary Epithelial Cell Media
67
Mammary Epithelial Cells
25
MATra-A Reagent
164
I, J, K, L
Indo-1 (pentaammonium salt)
115
MATra-S Immobilizer
164
Indo-1 (pentapotassium salt)
115
MATra-siRNA Reagent
164
Indo-1 (pentasodium salt)
115
McCoys 5A Media
86
Indo-1 AM
115
Media and Sera
60
Indo-1FF (K+ Salt)
116
Media for Primary Cells
61
Indo-1FF AM
116
Chondrocyte Media
61
Insect Cell Culture Media
87
Endothelial Cell Media
63
Insulin
99
82
Interleukin antibodies
169
Epithelial Cell Media
65
Interleukin ELISAs
175
Fibroblast Media
70
Interleukins, recombinant
179
Follicle Dermal Papilla Cell Media
72
118, 142
Hematopoietic Progenitor Cell Media
81
118, 142
Hepatocyte Media
73
JC-1 chloride
110
Keratinocyte Media
74
JC-1 iodide
110
Melanocyte Media
75
JNK Activated Cell Lysate
139
Mesenchymal Stem Cell Differentiation Media
80
139
80
139
Mesenchymal Stem Cell Growth Medium DXF
80
JNK Negative Control Cell Lysate
139
Mesenchymal Stem Cell Media
80
Keratinocyte Media
74
83
Keratinocytes
32
Mononuclear Cell Media
84
106
Myocyte Media
62
Leibovitz L-15 Medium
85
Osteoblast Media
76
L-Glutamine Solution
99
Pericyte Media
81
105
Preadipocyte/Adipocyte Media
77
L-JNK Peptide Inhibitor I
139
Skeletal Muscle Cell Media
78
L-JNKI1 Negative Control Peptide
139
Smooth Muscle Cell Media
79
114
Melanocyte Media
75
Lucifer Yellow CH lithium salt
114
Melanocytes
33
Lucifer Yellow CH potassium salt
114
MEM Alpha Media
86
Description
Page
Description
Page
MEM Amino Acids Solution
99
Nerve Terminal Red Staining Kit II
112
MEM D-Valine, w/o L-Glutamine
86
Neuro-DiI
111
MEM Media
86
Neuro-DiO
111
MEM NEAA Solution
99
Neurogenic Differentiation Medium
80
MEM Vitamin Solution
99
NHDF
27
122
NHEK
32
Mesenchymal Stem Cell Adipogenic Differentiation Medium
80
NHEM
33
Mesenchymal Stem Cell Chondrogenic Differentiation Medium
80
Nonyl Acridine Orange (or NAO)
110
80
122
Mesenchymal Stem Cell Neurogenic Differentiation Medium
80
Nucleic Acid Isolation
124
Mesenchymal Stem Cell Osteogenic Differentiation Medium
80
Octadecyl Rhodamine B Chloride (R18)
112
Mesenchymal Stem Cells
49
Okadaic acid
142
Mesenchymal Stem Cells from Adipose Tissue
49
Orange-fluorescent Cytoplasmic Membrane Staining Kit
111
Mesenchymal Stem Cells from Bone Marrow
49
Ordering and Technical Support
205
Mesenchymal Stem Cells from Umbilical Cord Matrix
49
Osteoblast Media
76
184
76
Mineralization Medium Osteoblasts
76
Osteoblasts
34
Mitochondria/Cytosol Fractionation Kit
122
Osteogenic Differentiation Medium
80
Mitochondrial Apoptosis Staining Kit
130
Oxidized Glutathione (GSSG)
121
124
MitoGreen
110
Mitomycin C
142
MitoRed
110
PBS
98
Mitsuhashi/Maramorosh Insect Medium
87
188
83
186
Monocytes
54
PCR Mycoplasma Test Kit I/RT; Variant A
186
84
PCR Mycoplasma Test Kit I/RT; Variant B
186
Mononuclear Cells
56
PCR Mycoplasma Test Kit III
186
Mononuclear Cells from Cord Blood
57
124
Mononuclear Cells from Peripheral Blood
56
Pellets
91
MQAE
117
Pen/Strep Solution
97
88
Pen/Strep/Fungizone Solution
97
189
Pericyte Media
81
189
Pericytes
51
Mycoplasma-EX
187
Pharmacidal Spray
189
Mycoplasma-EX Spray
189
Phorbol-12-myristate 13-acetate (PMA)
142
Myocyte Media
62
Phycobiliprotein Dyes
152
Placental Epithelial Cell Media
69
Placental Epithelial Cells
26
Plasmid Vectors
166
N, O
NAD/NADH Quantitation Kit
121
Pluronic F-127
114, 118
NADP/NADPH Quantitation Kit
121
Pluronic F-127, 10% in H2O
114, 118
Nasal Epithelial Cells
23
Pluronic F-127, 20% solution in DMSO
114, 118
NBD C6-ceramide
112
pPK-CMV-E1 Expression Vector Kit
166
NBD C6-sphingomyelin
112
166
NBD-DHPE
111
166
Nerve Terminal Green Staining Kit I
112
pPK-CMV-F1 Fusion Vector (C-GFP)
166
Nerve Terminal Green Staining Kit II
112
pPK-CMV-F2 Fusion Vector (N-GFP)
166
Nerve Terminal Green Staining Kit III
112
pPK-CMV-F3 Fusion Vector (C-Luc)
166
Nerve Terminal Green Staining Kit IV
112
pPK-CMV-F4 Fusion Vector (N-Luc)
166
Nerve Terminal Red Staining Kit I
112
pPK-CMV-R0 Reporter Vector (Blank)
166
197
Appendix
Alphabetical Index
Appendix
198
Alphabetical Index
Description
Page
Description
Page
pPK-CMV-R1 Reporter Vector (b-Gal)
166
PromoFluor-XXX-Goat Anti-Rabbit IgG
150, 173
pPK-CMV-R2 Reporter Vector (CAT)
166
148
pPK-CMV-R3 Reporter Vector (Luc)
166
109, 110
pPK-CMV-R4 Reporter Vector (GFP)
166
109, 110
pPK-CMV-R5 Reporter Vector (SEAP)
166
Prostate Smooth Muscle Cells
41
Preadipocyte Media
77
Protein and Antibody Labeling Kits
148, 174
Preadipocytes
35
Protein Extraction
122
Prestained Protein Ladder I
123
Protein Gel Stains
123
Prestained Protein Ladder II
123
Protein Size Markers
123
Primary Cells
10
Pulmonary Artery Endothelial Cells
16
PromoFectin
160
Pulmonary Artery Smooth Muscle Cells
38
160
Pulmonary Fibroblasts
28
160
Pulmonary Microvascular Endothelial Cells
21
PromoFectin-HUVEC
160
Purple-beta-D-Gal
108
PromoFectin-Insect
160
160
PromoFectin-Neuron
160
160
Rapamycin
142
PromoFectin-siRNA
160
94
PromoFluor Amino-Modified Label
147
Receptors, recombinant
179
PromoFluor Antifade Reagent
153
134
PromoFluor Carboxylic Acid
147
134
PromoFluor ChipPack
147
134
PromoFluor Maleimide
147
Red Multi-Caspase Staining Kit
134
PromoFluor NHS-Ester
147
Red-beta-D-Gal
108
PromoFluor-XXX-Goat Anti-Mouse IgG
150
Red-fluorescent Cytoplasmic Membrane Staining Kit
111
PromoFluor-430 Nick Translation Labeling Kit
149
Reduced Glutathione (GSSG)
121
149
Renal Cortical Epithelial Cells
26
151
Renal Epithelial Cell Media
68
151
Renal Epithelial Cells
25
149
Reporter Assays
107
149
Reporter Vectors
166
151
Resazurin, sodium salt
104
151
Resistin EDK, human
105
149
RH237
113
149
RH414
113
151
RH421
113
151
RH795
113
149
Rhod-2 (triammonium salt)
115
149
Rhod-2 (tripotassium salt)
116
151
Rhod-2 (trisodium salt)
116
151
Rhod-2 AM
116
149
Rhodamine 123
110
149
Rhodamine-DHPE
111
151
Rose-beta-D-Gal
108
151
Rosiglitazone
142
PromoFluor-XXX, phalloidin conjugate
150
R-Phycoerythrin
152
PromoFluor-XXX, streptavidin conjugate
152
RPMI 1640 Media
85
PromoFluor-XXX. biotin conjugate
152
RTU DNA Ladder I
125
PromoFluor-XXX-aadUTP
151
RTU DNA Ladder II
125
PromoFluor-XXX-Goat Anti-Human IgG
150, 173
RuBP-2S
114
Description
Page
Description
Page
RuBP-4S
114
Thapsigargin
142
RuBPS
114
TMRE
110, 113
TMRM
110, 113
TPEN
117
Trace Element Mix
99
S
Saphenous Vein Endothelial Cells
16
Tracheal Epithelial Cells
23
SCAS
112
Tracheal Smooth Muscle Cells
39
Schneiders Drosophila Medium
87
Transfection Reagents
156
SDIP/Europium for membrane fusion assay
114
Transferrin
99
Senescence Detection Kit
106
TRITC-DHPE
111
Sera
87
Trypan Blue Solution (0.5%)
110
Set of four Magnet Bar Plates
164
96
Skeletal Muscle Cell Media
78
Trypsin Solutions
96
36
Tyrphostin AG 1295
142
SkMC
36
Small Airway Epithelial Cell Media
66
Small Airway Epithelial Cells
24
Smooth Muscle Cell Media
79
Umbilical Artery Endothelial Cells
14
Smooth Muscle Cells
37
Umbilical Artery Smooth Muscle Cells
39
SOD Assay Kit
121
Umbilical Vein Endothelial Cells
13
Sodium bicarbonate Solution
98
Uterine Fibroblasts
29
Soluble Receptors, recombinant
179
Uterine Microvascular Endothelial Cells
21
Stable Glutamine Solution
99
Uterine Smooth Muscle Cells
41
Staurosporine
142
Villous Mesenchymal Fibroblasts
29
Stem and Blood Cells
48
Western Blotting Kits and Reagents
174
Stem Cell Research
170, 181
White Preadipocytes
35
Sulforhodamine 101
114
X-Gal
108
Sulforhodamine 101-a-bungarotoxin
112
Zinquin, ethyl ester
117
Sulforhodamine B
114
Zinquin, free acid
117
Sulforhodamine G
114
Superoxide Dismutase (SOD, human)
121
Supplements
99
SynapseGreen C1
112
112
112
SynapseGreen C3
113
113
113
113
113
SynapseRed C2M (FM5-95)
113
T
Tamoxifen citrate
142
TC Water
98
Tetrabromorhodamine 123, bromide
110
Tetramethylrhodamine-a-Bungarotoxin
113
Texas Red-DHPE
111
TexasRed PCR Labeling Kit
149
TexasRed-dUTP (PCR)
151
U, V, W, X, Z
199
Appendix
Alphabetical Index
200
International Distributors
Cat. Number
Albania
Page
Biomedica
Shpk
Zona 4, Godina 1
Kati 1, Ap. 1
1000 Tirana
Albania
Cat. Number
Product
Phone:
Page
Fax:
Email:
Internet:
+355-69 40 91 613
+355-45 00 681
a.selimi@bmgrp.al
www.biomedica.co.at
Banksia Scientific Company

3/160 Lytton Road
PO Box 529
Bulimba QLD 4171
Australia
Phone:
Phone:
Fax:
Email:
Internet:
1300 769944 (toll-free)

+61-7-39 02 30 00
+61-7-32 17 98 69
sales@banksiascientific.com.au
www.banksiascientific.com.au
Life Research Pty Ltd.

1 Dalmore Drive, Caribbean Park
Scoresby, Victoria 3179
Australia
Phone:
Fax:
Email:
Internet:
+61-3-97 64 25 66
+61-3-86 48 58 41
info@liferesearch.com
www.liferesearch.com
Austria
Biomedica Medizinprodukte GmbH & Co KG

Divischgasse 4
1210 Wien
Austria
Phone:
Fax:
Email:
Internet:
+43-1-29 10 75 6
+43-1-29 01 42 9
sales@bmgrp.at
www.biomedica.co.at
Bangladesh
KRISHGEN BioSystems
Unit Nos#318/319
3rd Floor
Shah & Nahar Ind. Premises
Dr E Moses Road
Worli
Mumbai 400018
India
Phone:
Fax:
Email:
Internet:
+91-22-66 37 29 90
+91-22-66 37 29 91
sales@krishgen.com
www.krishgen.com
Bosnia-Herzegovina
Biomedica d.o.o.
Zmaja od Bosne 4
71000 Sarajevo
Bosnia
Mobile:
Phone:
Fax:
Email:
Internet:
+387-66 90 60 81
+387-33 26 27 25
+387-33 26 27 26
biomedica@bmgrp.ba
www.biomedica.co.at
Bulgaria
Biomedica Bulgaria Ltd.

Sopharma Business Towers
5 Lachezar Stanchev Str.
Bl. B, Office 4-B6, Fl. 6
1766 Sofia
Bulgaria
Phone:
Fax:
Email:
Internet:
+359-2-44 72 833
+359-2-44 72 831
officeBg@bmgrp.eu
www.biomedica-bg.eu
Canada
CEDARLANE
4410 Paletta Court
Burlington, Ontario, Canada
L7L 5R2
Canada
Phone:
Fax:
Email:
Internet:
1-800-268-5058 (toll-free)
+1 289-288-0020
general@cedarlanelabs.com
www.cedarlanelabs.com
MJS BioLynx Inc.

300 Laurier Blvd.
Brockville, Ontario, Canada
K6V 5W1
Canada
Phone:
Fax:
Email:
Internet:
1-888-593-5969 (toll-free)
+1 613 342-1341
sales@biolynx.ca
www.biolynx.ca
China
Jiangsu Pu Nuo Sheng (PromoCell) Biotech Ltd.

Building 2, No.6 Dong Sheng Xi Road
National High Tech Development Park
Jiangyin, 214434
China
Phone:
+86-510-81 69 51 00
Fax:
+86-510-81 69 52 00
Email: info@promocell-china.com
Internet:
www.promocell-china.com
Croatia
Biomedica Dijagnostika d.o.o.

Paljetkova 2
10000 Zagreb
Croatia
Phone:
Fax:
Email:
Internet:
+385 1 888 5727

+385 1 888 5728
biomedica-dijagnostika@bmgrp.hr
www.biomedica.co.at
Czech Republic
BIOMEDICA CS, s.r.o.

Meteor Centre Office Park B
Sokolovsk 100/94
186 00 Praha 8
Czech Republic
Phone:
Phone:
Fax:
Email:
Internet:
+420 283 931 485

+420 545 214 915
+420 283 932 507
info@biomedica.cz
www.biomedica.cz
Denmark
PromoCell GmbH
Sickingenstr. 63/65
69126 Heidelberg
Germany
Phone:
+49 6221 - 649 34 0
Fax:
+49 6221 - 649 34 40
Email: lorna.whyte@promocell.com
Internet:
www.promocell.com
Finland
Biotop Oy
Tykistkatu 6B 3
20520 Turku
Finland
Phone:
Fax:
Email:
Internet:
+358-2-241 00 99
+358-2-241 01 11
info@biotop.fi
www.biotop.fi
Hungary
Biomedica Hungria Kft.

Ganz u. 16
1027 Budapest
Hungary
Phone:
Fax:
Email:
Internet:
+361-22 53 85 0
+361-20 12 68 4
biomed@biomedica.hu
www.biomedica.hu
Australia
Appendix
Product
India
DSS Takara Bio India Pvt. Ltd.

A-5 Mohan Co-op Indl. Estate
Mathura Road
New Delhi-110044
India
Phone:
Fax:
Email:
Email:
+91-33-30886717/21
+91-269593821
orderinfo@dsstakarabio.com
orderinfo@dssimage.com
KRISHGEN BioSystems
Unit Nos#318/319, 3rd Floor
Dr E Moses Road
Worli
Mumbai 400018
India
Phone:
Fax:
Email:
Internet:
+91-22-66 37 29 90
+91-22-66 37 29 91
sales@krishgen.com
www.krishgen.com
Indonesia
KRISHGEN BioSystems
Dr E Moses Road
Worli
Mumbai 400018
India
Phone:
Fax:
Email:
Internet:
+91-22-66 37 29 90
+91-22-66 37 29 91
sales@krishgen.com
www.krishgen.com
Israel
Biological Industries Ltd.

Kibbutz Beit Haemek, 25115
Israel
Phone:
Fax:
Email:
Internet:
+972-4-996-0595
+972-4-996-0631
info@bioind.com
www.bioind.com
Italy
VWR International PBI S.r.l.

Via San Giusto, 85
20153 Milano
Italy
Phone:
Fax:
Email:
Internet:
+39-02-48 77 91
+39-02-40 09 00 10
info@it.vwr.com
it.vwr.com
Japan
Funakoshi Co. Ltd.

9-7 Hongo 2-chome
Bunkyo-ku
Tokyo
Japan 113-0033
Phone:
Fax:
Email:
Internet:
+81-3-56 84 16 20
+81-3-56 84 17 75
reagent@funakoshi.co.jp
www.funakoshi.co.jp
TaKaRa Bio Inc.

Seta 3-4-1
Otsu, Shiga 520-2193
Japan
Phone:
Fax:
Internet:
+81-77-543 7247
+81-77-543 9254
www.takara-bio.co.jp
Korea
Sungwoo Life Science Co., Ltd.

Samsung BLDG 4th Floor 560-5
Uijeongbu 2Dong Uijeongbu-City
Kyeonggi-Do
South Korea
Phone:
Fax:
Email:
Internet:
+82-2-985-7471
+82-31-876-9149
sungwoo@sungwools.com
www.sungwools.com
Latvia
SIA DIAMET
Aizkraukles iela 23
Riga, 1006
Latvia
Phone:
Fax:
Email:
Internet:
+371 26 47 47 64
+371 67 54 33 31
diamet@diamet.lv
www.diamet.lv
Macedonia
Biomedika Dijagnostika dooel

Ul. llindenska br. 103 lokal 2 i 3
1000 Skopje
Macedonia
Phone:
Fax:
Email:
Internet:
+389 703 62 676

+389 232 22 404
v.peeva@bmgrp.mk
www.biomedica.co.at
Malaysia
Biomarketing Services SDN BHD

21, Jalan 4/62A
Bandar Menjalara, Kepong
52200 Kuala Lumpur
Malaysia
Phone:
Fax:
Email:
Internet:
+603 6273 3068

+603 6272 0093
info@biomarketing.com.my
www.biomarketing.com.my
Nepal
KRISHGEN BioSystems
Dr E Moses Road
Worli
Mumbai 400018
India
Phone:
Fax:
Email:
Internet:
+91-22-66 37 29 90
+91-22-66 37 29 91
sales@krishgen.com
www.krishgen.com
Netherlands
Bio-Connect B.V.
Begonialaan 3a
6851 TE Huissen
Netherlands
Phone:
Fax:
Email:
Internet:
+3126 326 4450

+3126 326 4451
info@bio-connect.nl
www.bio-connect.nl
New Zealand
Banksia Scientific Company

12 Brigade Street
Airport Oaks
Auckland
New Zealand
Phone:
Fax:
Email:
Internet:
+64-9-921 26 26
+64-9-921 26 27
sales@banksiascientific.co.nz
www.banksiascientific.co.nz
Life Research Pty Ltd.

Level 1, 5 Lakeside Drive
Burwood East Victoria 3151
Australia
Phone:
Fax:
Email:
Internet:
+61-3-98 03 00 45
+61-3-86 48 58 41
info@liferesearch.com
www.liferesearch.com
201
Appendix
Appendix
202
Norway
PromoCell GmbH
Sickingenstr. 63/65
69126 Heidelberg
Germany
Phone:
+49 6221 - 649 34 0
Fax:
+49 6221 - 649 34 40
Email: lorna.whyte@promocell.com
Internet:
www.promocell.com
Poland
Biomedica Poland sp. z.o.o.

Ul. Raszyska 13
05-500 Piaseczno
Poland
Phone:
Fax:
Email:
Internet:
+48-22 73 75 996
+48-22 73 75 994
biomedica@bmgrp.pl
www.biomedica.pl
Portugal
LABCLINICS, S.A.
Industria, 54
08025 Barcelona
Spain
Phone:
Fax:
Email:
Internet:
+34-93-446 47 00
+34-93-348 10 39
info@labclinics.com
www.labclinics.com
Romania
S.C.C.Y.B.E.R. Srl Romania

Str. Smaranda Braescu, nr. 20D
Sector 1, Cod 014203
70941 Bucharest
Romania
Phone:
Phone:
Fax:
Email:
Internet:
+40-37 27 66 844
+40-37 27 66 845
+40-21 23 22 204
gabriela.matei@cybermed.ro
www.cyber-srl.ro
Serbia
BIOMEDICA MP d.o.o.
Cara Dusana 214
11080 Zemun
Serbia
Phone:
Fax:
Email:
Internet:
+381 11 6301 882

+381 11 6301 883
office@biomedicamp.com
www.biomedica.co.at
Singapore
Biomed Diagnostics Pte. Ltd.

18 Boon Lay Way
#05-105-108 TradeHub 21
Singapore 609966
Phone:
Fax:
Email:
Internet:
+65-6-298 43 47
+65-6-298 47 23
sales@biomed.com.sg
http://www.biomed.com.sg/main.htm
Singlab Technologies Pte. Ltd.

1003 Bukit Merah Central #07-50
Inno. Centre
Singapore 159836
Phone:
Fax:
Email:
Internet:
+65-6-272 07 88
+65-6-270 71 28
PromoCell@singlab.com.sg
www.singlab.com.sg
Slovakia
Biomedica Slovakia s.r.o.

Drobnho 27
84101 Bratislava
Slovakia
Phone:
Fax:
Email:
Internet:
+421-2-69 30 99 01
+421-2-69 30 99 08
biomedica@biomedica.sk
www.biomedica.sk
Slovenia
Biomedis M.B.
Slokanova ulica 12
2000 Maribor
Slovenia
Phone:
Fax:
Email:
Internet:
+386-2-47 16 300
+386-2-47 16 304
info@biomedis-mb.si
www.biomedis-mb.si
South Korea
Sungwoo Life Science Co., Ltd.

Samsung BLDG 4th Floor 560-5
Uijeongbu 2Dong Uijeongbu-City
Kyeonggi-Do
South Korea
Phone:
Fax:
Email:
Internet:
+82-2-985-7471
+82-31-876-9149
sungwoo@sungwools.com
www.sungwools.com
Spain
LABCLINICS, S.A.
Industria, 54
08025 Barcelona
Spain
Phone:
Fax:
Email:
Internet:
+34-93-446 47 00
+34-93-348 10 39
info@labclinics.com
www.labclinics.com
Sri Lanka
KRISHGEN BioSystems
Unit Nos#318/319
3rd Floor
Dr E Moses Road
Worli
Mumbai 400018
India
Phone:
Fax:
Email:
Internet:
+91-22-66 37 29 90
+91-22-66 37 29 91
sales@krishgen.com
www.krishgen.com
Sweden
Mediqip AB
Frrdsvgen 10
141 46 Huddinge
Sweden
Phone:
Fax:
Email:
Internet:
+46-8-448 10 10
+46-8-448 10 75
info@mediqip.se
www.mediqip.se
Taiwan
Biochiefdom International Co. Ltd.

1F, No 50, Yishou St.
Wenshan District
Taipei 11659
Taiwan
Phone:
Fax:
Email:
Internet:
+886-2-29 37 00 88
+886-2-29 37 97 17
info@bio-chief.com.tw
www.bio-chief.com.tw
Thailand
Biomed Diagnostics (Thailand) Co. Ltd.

1518/4 Kengrungruengchai Tower, 7th Floor
Wong Sawang, Bang Sue,
Bangkok 10800
Thailand
Phone:
Fax:
Email:
Internet:
+66-2-879 60 26
+66-2-879 60 65
nan@biomedthai.com
www.biomedthai.com
For an up-to-date list of all PromoCell and PromoKine distributors, please see www.promocell.com/contact
Terms and Conditions
203
General Terms
Delivery
1. These conditions of sale and delivery

apply to all contracts of supply between
us and our customers, even when in individual cases they are not referred to.
2. A customers inconsistent conditions
of purchase shall have no validity, even
when we do not expressly reject such
conditions.
3. The customer is deemed to agree to
these conditions of sale and delivery
upon unconditional acceptance of the
goods supplied.
4. Rights of the customer arising during
the course of business with us are not
transferable.
5. Any agreement that differs from these
conditions of sale and delivery, or any
recognition of the customers contradictory purchase conditions, requires our
express confirmation in writing.
1. Delivery times will be specified after

careful co-ordination. They are however
not legally binding. Claims for compensation due to delayed or non-delivery are
excluded.
2. If a delivery date specified by us is
exceeded by more than two weeks, the
customer is entitled to give us a further
two week time limit for delivery. Should
the delivery not be carried out by the
end of the additional two week delivery
period, the customer is entitled to rescind the contract. The rescission must
be made promptly and in writing, at the
latest within a week of the expiry of the
additional two week delivery period.
3. All deliveries take place at the customers risk. The passing of risk takes place
on the handing over of the goods to the
carrier or haulier. The careful choice of
dispatch method and means of transport
is at our discretion with exclusion of any
and all liability.
4. Deliveries in part are permitted. They
are to be regarded as separate deliveries.
Offers
1. In the absence of a declaration to the
contrary, we are not bound by any offers
we make.
2. Supply contracts only take effect when
confirmed by us in writing.
3. Any inquiries or complaints concerning
confirmation are to be made promptly in
writing.
4. Assurances and assured properties
only exist when they are expressly described as such. The descriptions and
other statements in our catalogues and
price lists are accurate to the best of our
knowledge and belief. Under no circumstances may any assurances as to properties be derived from such descriptions
and statements.
Prices
1. Our prices quoted in the current price
list are net prices, VAT (value added tax)
not included.
2. VAT is charged according to the applicable laws in effect.
3. Charges for delivery, cooling and insurance are indicated in the valid price
list.
4. PromoCell prices are subject to change
without prior notice.
Payment
1. Payment is to be made without any
deductions within 14 days of delivery
and remittance of the bill.
2. Default of payment will result in legal
proceedings upon the expiry of the payment period without further warning. In
this case, or in the case of a warning, we
are entitled to interest for late payment
at the current normal bank borrowing
rate, or alternatively at a rate of 5%
above the current discount rate of the
Deutsche Bundesbank.
3. A cash discount is only permitted
when expressly stated on the bill and
when payment is made within the stated
period.
4. An offset against or demand for
payment is only permitted when the
customer has an undisputed or legally
established counterclaim.
5. If our conditions of payment are not
complied with or if justifiable doubts
exist regarding the customers ability or
willingness to pay, we are entitled to
rescind the contract and to demand the

return of the delivered goods, the costs
of delivery to be borne by the customer.
In addition, all time outstanding claims
will become due.
Justifiable doubts referred to above
include, but are not restricted to: unfavourable information, deterioration of
financial circumstances, initiation of insolvency or bankruptcy proceedings, execution measures against the customer,
protest of bills, and default of payment
for any other reason.
6. If the customer does not take delivery of properly delivered goods, we are
entitled, after setting an additional time
limit, to rescind the contract, or to damages for non-compliance with the contract. In the latter case we are entitled to
demand either damages of 25% of the
sales price without proof, or the actual
amount of damages.
Complaint
1. Any complaint regarding the goods,
such as defects, lack of assured properties, wrong delivery and over- or underdelivery are, after immediate discontinuation of use, to be reported in writing.
2. In the case of wrong delivery, the
goods will be taken back at our expense
and replaced with the goods ordered.
Excess deliveries may similarly be sent
back to us at our expense. In the case of
under delivery, the customer may choose
between a reduction in payment or complete fulfilment of the order.
3. In the case of delivery of faulty goods
we are entitled to replace the faulty
goods with goods free of faults. Should
the replacement delivery also be faulty,
the customer may choose between a
reduction in payment or cancellation of
the contract. Claims for compensation
are excluded except in the case of lack of
assured properties.
4. The right to complain expires in the
case of obvious or apparent defects two
weeks after receipt of the goods, and
in the case of non-apparent defects six
months after receipt of the goods.
5. Goods may only be returned by arrangement with us and with the issue
of a return number. The goods must be
Appendix
Appendix
204
properly packed. If returned goods are

damaged due to the fault of the customer, the customer is obliged to pay
compensation for such damage.
6. In the case of delivery to traders the
customer is to inspect the goods without delay upon receipt and to report
any complaints to us immediately. If the
customer fails to make such a report
the goods will be deemed approved. In
the case of a fault that is not apparent
upon inspection, the complaint must be
reported immediately upon discovery of
the fault, otherwise the goods will be
deemed approved as regards such fault.
Use of Goods
1. Goods delivered by us are intended
exclusively for the purposes of research
and may not be used on human beings,
animals, in the household, or for any
private use. We emphasise that the use
of our products for diagnostic or therapeutic purposes is subject to current legal
regulations.
2. We do not accept any liability for the
improper use of our products. We deliver
only to commercial enterprises, resalers
and public research, development and
teaching establishments. Legal regulations require us to carefully check orders
and to refuse delivery when there are
signs of improper use of our products.
Orders from students or other private
individuals must be given from an official
office of their institute or firm.
3. We expressly oppose research or any
uses which run counter to ethical, legal
or political standards or principles within
Germany and/or the import country, or
whose ethical justification is the subject
of public debate and dispute in the respective country (e.g. certain cloning
experiments).
We do not accept any liability for such
research or use by our customers, or
by third parties who have received our
products from our customers.
Liability
1. All products delivered by us are only
to be handled by trained personnel who
are well aware of any potential dangers.
Should any liability for compensation
arise as a result of damage caused by

breach of this condition, the customer
shall be liable to pay any such compensation. In all cases, current safety regulations are to be observed, especially in the
case of cells of human or animal origin,
which always represent a potential risk
of infection. The lack of a danger symbol
does not mean that the substances concerned are harmless.
2. We accept no responsibility for damage, loss, or injury caused by incorrect
handling or use in the household, on
humans, or on animals. In as far as we
have made confirmation of delivery dependent upon a specified use of certain
products, the customer accepts full liability for any damage suffered by us as
a result of the customers incorrect use
of the product. In the case of products
which may only be used in accordance
with legal or official regulations, the customers order simultaneously counts as
a declaration that the products will be
used for a specific purpose in the above
sense.
3. Our customers are required without
exception to apply the laboratory guidelines of the professional association of
the chemical industry when dealing with
our products.
4. Passing on of our products to private
individuals is not permitted. If as a result
of a breach of this condition for whatever reason we should receive a claim for
compensation from a private individual,
we shall have a right of redress against
the customer.
5. PromoCell provides no warranties as
to the accuracy of the information contained in printed materials like product
informations, catalogs, instruction manuals, etc. and shall bear no responsibility
or liability whatsoever for any errors,
faults or omissions in the information.
ers or employees. We accept no liability

for damage caused by the behaviour of
companies or individuals which sell our
products.
3. The sale of our products under their
trade names for further commercial use
does not mean that these products and
their trade names may be freely used
in general circulation. Some products,
their production and use or their trade
names are patented or protected trade
names. The further commercial use of
such products and their trade names occurs as regards a third party under the
exclusive responsibility and liability of
the customer.
Indemnification
Managing Director: Dirk Httner

AG Mannheim HRB 335480
1. In addition to section complaint,

claims by the customer are excluded, in
so far as they are not based upon intention or gross negligence.
2. In the case of delivery to a trader this
exclusion also applies to any damage
caused by the intention or gross negligence of our business associates, carri-
Miscellaneous
1. German law shall apply to all contracts
entered into by us, including those for
deliveries abroad. We expressly reject the
application of the Vienna UN-agreement
relating to international sales contracts
of 11. 04. 80.
2. In the case of contracts with traders
who do not belong to the traders specified in 4 of the code of commercial law,
the place for fulfilment of contracts and
the court of jurisdiction shall be Heidelberg.
3. If any of the above conditions is held
to be invalid, the validity of any of the
other conditions shall not be affected.
4. Only the German version of this Terms
of Delivery and Payment is valid. This
translation shall only serve as assistance
for our customers. You can get a German
version on request.
PromoCell GmbH
Sickingenstr. 63/65
69126 Heidelberg
Germany
205

Ordering Information
Phone
Fax
Online
Our customer service representatives are

ready to assist you with ordering Monday
through Friday
Please copy the fax order form on the last

page and send it to the following fax numbers:
USA/Canada
1 866 251 2860 (toll free)
USA/Canada
1 866 827 9219 (toll free)
Online ordering is available 24 hours/day,

7 days per week. Our online ordering system is secure, fast, and convenient.
Check out www.promocell.com or www.
promokine.info for more information.
Germany
0800 776 66 23 (toll free)
Germany
0800 100 83 06 (toll free)
France
0800 90 93 32 (toll free)
France
0800 90 27 36 (toll free)
United Kingdom
0800 96 03 33 (toll free)
United Kingdom
0800 169 85 54 (toll free)
Switzerland
+49 6221 649 34 0
Switzerland
+49 6221 649 34 40
Netherlands
+49 6221 649 34 0
Netherlands
+49 6221 649 34 40
Email
Please send an e-mail to
order@promocell.com and include the
following information:
Catalog number, name, size and quantity
Shipping and billing address
Contact person`s name and telephone
number
Other Countries
Please see www.promocell.com/distributor or call +49 6221 649 34 0
PromoCell GmbH directly supplies to Belgium, Canada, France, Germany, Ireland,

Liechtenstein, Luxembourg, Monaco,
Netherlands, Switzerland, United Kingdom, and the United States.
All deliveries are carried out using our
recommended carrier, unless an alternative agreement has been arranged
with the customer. Delivery time and
shipment costs are extremely favorable. Our local sales representatives
and our multilingual customer service
are looking forward to serving you.
For
current
information
about
PromoCell and PromoKine distributors
see www.promocell.com/distributor or
contact us via e-mail or phone.
Technical Support
Providing excellent technical support
for our products is our top priority. Our
technical service team relies on years of
laboratory experience to assist you with
product selection and advice, to maximize product performance.
You may reach our technical support via
telephone/fax (see numbers on the reverse side of the catalog) or by sending
an e-mail to: tech.support@promocell.com
Technical support is completed by our

online Knowledge Base:
www.promokine.info/knowledge-base
It provides reference literature, application notes, and a library of technical
questions and answers related to our
products.
Product Use
Products are sold for research use only
and are not intended for use in diagnostic procedures or for therapeutic and
medical purposes.
Trademarks Used in this Catalog

The trademarks, logos, and service marks
(collectively the Trademarks) displayed
in this catalog, including but not limited
to those contained in the list below are
trademarks or registered trademarks of
PromoCell GmbH in Europe, the U.S.
and other countries. All other brands
and product names are trademarks or
registered trademarks of their respective
companies. Nothing contained in this
catalog should be construed as granting,
by implication, estoppel, or otherwise,
any license or right to use any trademarks
displayed in the catalog without the

permission of PromoCell GmbH or such
third party that may own the trademarks
displayed in the catalog. The misuse of
the trademarks displayed in the catalog,
or any other content in the catalog, is
strictly prohibited. Please be advised that
PromoCell GmbH will aggressively enforce its intellectual property rights to
the fullest extent of the law, including
the seeking of criminal prosecution.
PromoCell (PromoCell GmbH)
PromoFectin (PromoCell GmbH)
PromoFluor (PromoCell GmbH)
PromoKine (PromoCell GmbH)
DetachKit (PromoCell GmbH)
SupplementPack (PromoCell GmbH)
SupplementMix (PromoCell GmbH)
Cell Culture Assistant (PromoCell GmbH)
ABI Prism
Alexa Fluor
Cell Lab Quanta SC
Cepheid Smart Cycler
CyTM
LightCycler
Mastercycler
MitoCaptureTM
Rhodamine Green
RNAlater
Texas Red
Appendix
To place an order please use any of the

following convenient options:
206
Notes
Appendix
Notes
Notes
207
Appendix
Notes
208
Notes
Appendix
Notes
Fax Order Form

Shipping Address
Mr.
The Cell Culture Experts

www.promocell.com
Invoice Address (if different from shipping address)

Ms.
Dr.
Prof.
Mr.
Ms.
First Name
First Name
Last Name
Last Name
Street, No
Street, No
ZIP, City
ZIP, City
Country
Country
Dr.
Prof.
Phone
E-mail
Order Details
Catalog Number
Product Name
Quantity
Your Cell Research Partner

www.promokine.info
Please write in capital letters
Training Courses
www.promocell-academy.com
Please visit our websites regularly for upcoming new products and training courses
Please send me a copy of the
Please send me your newsletters
PromoCell catalog
PromoCell
PromoKine catalog
PromoKine
PromoCell Academy catalog
PromoCell Academy
Date, Signature
PromoCell GmbH
Sickingenstr. 63/65
69126 Heidelberg
Germany
USA/Canada
Phone:
1 866 251 2860 (toll free)
Fax:
1 866 827 9219 (toll free)
Deutschland
Telefon: 0800 776 66 23 (gebhrenfrei)
Fax:
0800 100 83 06 (gebhrenfrei)
France
Tlphone: 0800 90 93 32 (ligne verte)
Tlfax:
0800 90 27 36 (ligne verte)
2013 | 2014
2013 | 2014
United Kingdom
Phone:
0800 96 03 33 (toll free)
Fax:
0800 169 85 54 (toll free)
Switzerland
Phone:
+49 6221 649 34 0
Fax:
+49 6221 649 34 40
Netherlands
Phone:
+49 6221 649 34 0
Fax:
+49 6221 649 34 40
Other Countries
Phone:
+49 6221 649 34 0
Fax:
+49 6221 649 34 40
Email: info@promocell.com
info@promokine.info
www.promocell.com
Human Primary Cells & Media

Stem and Blood Cells & Media
Cell Biology Products

PromoCellKatalog2013 Lowres

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PromoCell GmbH

Cell Culture & Cell Biology

Cell Culture & Cell Biology

Human Primary Cells & Media

Fax Order Form

The Cell Culture Experts

Invoice Address (if different from shipping address)

Your Cell Research Partner

Please write in capital letters

Please send me a copy of the

Please send me your newsletters

PromoCell Academy catalog

Human Primary Cells

Human Stem and Blood Cells

Media and Sera

Reagents and Supplements

Antibodies and ELISAs

Cytokines and Growth Factors

Microbial Detection and Elimination

PromoCell Ethical Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Human Primary Cells

Overview Human Primary Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Human Stem and Blood Cells

Overview Human Stem and Blood Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Media and Sera

Reagents and Supplements

PromoFectin Transfection Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Antibodies and ELISAs

Cytokines and Growth Factors

10.1 Mono- and Polyclonal Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

11.1 Cytokines, Growth Factors, Hormones, and soluble Receptors . . . . . . . . . . . 179

Microbial Detection and Elimination

12.1 Mycoplasma Test Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PromoCell Ethical Standards

PromoCell is the original manufacturer

the highest ethical and legal standards.

Extract from The Convention of Human Rights and Biomedicine:

The tissue used by PromoCell for the

Extract from The Declaration of Helsinki:

At PromoCell we continuously work to improve our products and services.

Your PromoCell Team

Discover our Knowledge Base

Information at your fingertips

Sections of the Knowledge Base include:

As scientists, we know that research is

To assist you with this, PromoCell has

You can find detailed answers to many

In the section Application Notes you

To access the PromoCell Knowledge Base, please

To access the PromoKine Knowledge Base, please

We appreciate your feedback

We thank you in advance for providing

Human Primary Cells

Human Primary Cells

Endothelial Cells (Large Vessels) . . . . . . . . . 13

Aortic / Coronary Artery . . . . . . . . . . . . . . . 15

Pulmonary Artery / Saphenous Vein . . . . . . 16

Endothelial Cells (Microvascular) . . . . . . . . 17

Bronchial / Small Airway . . . . . . . . . . . . . . . 24

Renal Cortical / Placental . . . . . . . . . . . . . . 26

Pulmonary / Aortic Adventitial / Cardiac . . 28

Uterine / Villous Mesenchymal . . . . . . . . . . 29

Follicle Dermal Papilla Cells . . . . . . . . . . . . . 30