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College of Medicine
Biochemistry Practical for 2nd MBBS
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Determination of Creatinine
Sample: -------------------------------------Wave length: -----------------------------------------Absorbance of the unknown sample: ---------------------------Absorbance of the standard: -----------------------------------Calculation
3. What are the factors that may affect the creatinine test result.
Structure of creatinine
Metabolism of creatinine
Creatine phosphate is used in skeletal muscle contraction. Creatinine is a breakdown
product of creatine. People with greater muscle mass produce a correspondingly greater
amount of creatine and have higher levels of serum creatinine than smaller people or
people with less muscle mass. Because creatinine is excreted by the kidneys, its level in
the blood is considered an indicator of kidney health. When the kidneys are functioning
normally, the blood creatinine level is relatively constant day to day, though minor
increases can occur after a high-protein meal.
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