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5(7):417-419
RESEARCH
Abstract
Aim: An experiment was conducted to study the haemato-biochemical alterations induced by Diclofenac Sodium toxicity.
Materials and Methods: 48 Swiss albino mice of either sex, divided uniformly into four different groups. The mice of group I
received only deionised water as control while, group II, III and IV were given Diclofenac sodium @ 2.37 mg/kg B.W, 4.75
mg/kg B.W, 9.5 mg/kg B.W orally for 28 days.
Results: In dose dependant significant reductions in TEC, Hb, PCV, MCV, MCHC were observed. No significant change was
observed in total WBC count in both the sexes. However, relative values of leukocytes indicated relative neutrophilia and
relative lymphopenia in higher group. Biochemically dose dependant significant changes were observed for AST, ALT, Total
bilirubin, Total protein, Albumin, Globulin, Cholesterol, Urea, Creatinine and Uric acid in male and female animals.
Conclusion: The present study indicates hepatobilliary, nephric and gastrointestinal toxicity in albino swiss mice due to
Diclofenac Sodium Toxicity.
Key words: Biochemical, Haematology, Diclofenac sodium, Mice
To cite this article: Basavraj SB, Fefar DT, Prajapati KS, Jivani BM, Thakor KB, Patel JH, Ghodasara DJ, Joshi BP,
Undhad VV (2012) Haemato-biochemical alterations induced by Diclofenac sodium toxicity in Swiss albino mice,
Vet World, 5(7): 417-419, doi: 10.5455/vetworld.2012.417-419
Introduction
417
Group I
Male
TEC(106/L)
10.070.05
Hb(g/dl)
14.220.16
PCV(%)
40.250.67
MCV(fl)
55.850.71
MCH(pg)
16.720.75
MCHC(%)
27.200.81
TLC(X103/l)
8.400.08
Differential Leucocyte count
Neutrophils
31.050.67
Lymphocytes
64.950.67
Monocytes
1.730.10
Eosinophils
1.780.13
Basophils
0.530.14
Female
9.960.22
14.200.35
39.580.36
56.981.63
17.880.89
27.631.20
8.150.17
9.830.15
14.380.24
39.830.60
58.331.49
18.240.16
31.172.67
8.250.19
10.010.19
13.670.21
41.170.95
57.351.94
19.200.39
28.251.88
7.880.12
6.880.11**
12.820.62*
35.171.60*
56.801.94
18.030.99
31.452.51
7.950.27
9.020.35
12.850.63
37.171.30
54.823.28
18.820.10
27.851.49
8.240.14
6.350.06**
10.951.00**
32.851.82**
60.401.91*
17.930.60
23.001.71*
7.900.08
7.710.16**
10.070.89**
32.382.25**
63.051.67*
18.750.13
20.501.71*
7.750.14
30.671.50
65.01.46
1.850.24
1.770.09
0.680.26
29.00.77
67.00.77
1.830.10
1.580.10
0.580.12
29.830.91
66.170.91
2.100.21
1.400.13
0.500.13
34.831.54*
61.171.54*
1.450.18
1.480.09
1.070.24
36.081.47*
59.921.47*
2.130.18
1.350.19
0.520.08
36.500.76**
57.501.38**
1.520.21
1.800.22
0.680.23
37.420.45**
58.580.45**
1.800.10
1.630.24
0.570.19
Table-2: Serum biochemical parameters (Mean SE) Swiss albino mice exposed with Diclofenac sodium
Parameters
Group I
Male
AST (IU/L)
ALT (IU/L)
69.551.45
51.822.58
Total Bilirubin (mg/dL) 0.110.012
Total Protein (g/dL) 7.030.09
Albumin (g/dL)
4.280.17
Globulin (g/dL)
2.750.19
Glucose (mg/dL)
103.930.90
Cholesterol (mg/dL) 66.070.79
Urea (mg/dL)
21.140.77
Creatinine (mg/dL) 1.500.17
Uric Acid (mg/dL)
1.050.06
Female
74.222.54
45.174.53
0.130.03
7.370.12
4.120.12
3.250.16
114.03.66
72.84.67
31.701.51
1.040.27
0.880.09
73.754.39
54.233.08
0.160.019
6.820.14
4.230.11
2.580.22
102.572.12
66.130.69
20.173.59
1.510.09
1.200.09
95.8211.40*
78.508.40**
0.530.08**
4.270.17**
2.780.30**
1.480.26**
107.652.65
46.972.19**
49.432.50**
4.400.34**
3.00.54**
77.501.80
48.822.50
0.180.02
7.410.18
4.450.14
2.960.28
121.6711.09
702.7
33.501.98
1.250.23
0.930.09
77.335.50
68.947.32*
0.540.05**
4.850.34**
3.170.25**
1.680.27**
102.371.66
64.682.48
26.283.20
2.070.20*
2.180.48*
72.673.00
77.945.23**
0.320.04**
7.070.26
3.980.46
3.080.53
109.674.40
63.661.28
42.833.85*
1.910.24*
2.00.47*
98.334.99**
119.675.69**
0.430.1**
5.380.45**
3.550.12**
1.830.42**
1053.83
46.962.19**
56.792.45**
2.810.29**
2.410.32**
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Conclusion
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Acknowledgements
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Competing interest
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References
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