Professional Documents
Culture Documents
2 Troubleshooting
3 - Parts Lists
4 - Removal & Replacement
5 - Verification Procedures
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Table of Contents
1.1 Proprietary Information . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 Revision Log. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.3 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Service Manual Section Descriptions . . . . . . . . . . . . . 3
Section 1: Introduction & General Data . . . . . . . . . . . . 3
Section 2: Troubleshooting . . . . . . . . . . . . . . . . . . . . . . 3
Section 3: Parts Lists . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Section 4: Removal & Replacement . . . . . . . . . . . . . . . 3
Section 5: Verification Procedures . . . . . . . . . . . . . . . . 3
Section 6: Preventive Maintenance & Total Call. . . . . . 3
1.4 CELL-DYN 3200 Operators Manual . . . . . . . . . . . . . . 3
1.5 Warranty Information. . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.6 System Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
The CELL-DYN 3200 Hematology System . . . . . . . . . . 4
System External Components . . . . . . . . . . . . . . . . . . . 4
System Internal Components . . . . . . . . . . . . . . . . . . . . 5
1.7 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Signal Words and Graphic Warning Conventions . . . 5
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Graphic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Biosafety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.8 CELL-DYN 3200 Hematology Parameters . . . . . . . . . . 9
1.9 System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . 10
CELL-DYN 3200 SYSTEM SERVICE MANUAL
1-1
Revision Log
Revision
Date
Pages Revised/Added
TSBs Incorporated
ISAs Incorporated
August
Complete Revision
1-2
Introduction
Section 1: Introduction
This section contains an introduction to the system, information
on the use of the manual, warranty information, safety
considerations, hematology parameters, and system
specifications.
1-3
System Reagents
Isotonic Diluent
Diluent/Sheath
Hemoglobin Lyse
WBC Lyse
System Controls & Calibrators
1-4
Flow Panel
Optics Bench
Power Supplies
Measurement Electronics
Computer Electronics
A/D & D/A Electronics
Air Supply
Personal Computer Module
Tube Manipulation Hardware
Graphic Conventions
1.7 Safety
Operation, maintenance, and servicing of hematology systems
may expose individuals to potential safety and health hazards.
All work must be performed in accordance with procedures
described in the Abbott Operators and Service Manuals, or as
directed by an Abbott Technical Support Representative.
Warnings are inserted in this manual to alert personnel to
potential hazards. The standard warning conventions including
signal words (e.g. Caution) and symbols are described below.
1-5
Biosafety
:$51,1*=#327(17,$/#%,2+$=$5' Consider
all clinical specimens, reagent controls, surfaces, or
components that contain or have contacted human
blood or serum as potentially infectious. Wear gloves,
lab coats and safety glasses, and follow other
biosafety practices as specified in the OSHA
Bloodborne Pathogen Rule (29CFR Part 1910.1030)
or other equivalent biosafety procedures.
:$51,1*=#327(17,$/#%,2+$=$5'#The
aspiration needle/probe is sharp and potentially
contaminated with infectious materials. Avoid contact
with the tip of the needle/probe.
1-6
Electrical Safety
:$51,1*= (/(&75,&$/#+$=$5' When the
instrument is powered and protective covers are
removed, there are exposed electrical systems that
could startle or seriously injure personnel upon
contact. Use appropriate safety precautions to prevent
body and/or tool contact with live electrical
components, especially power supplies.
Laser Safety
DANGER: LASER LIGHT Avoid direct exposure to
beam. Do not look directly into the laser beam or any
reflection of the beam from a mirror-like surface.
When the access door, instrument top cover, or other
inner protective cover is removed, (Helium-Neon)
laser power up to 10 mW continuous wave at 632.8
nm in a beam with 1 mR divergence could be
accessible in the interior of the optics bench. This
amount of energy, with insignificant attenuation with
distance, is sufficient to cause eye damage.
32109
Figure 1-1
1-7
Figure 1-2
1-8
.CUGT
9CTPKPI
.CDGN
RBC
Hemoglobin Concentration
HGB
Hematocrit
HCT
MCV
MCH
Table 1-3
Figure 1-4
Abbreviation
MCHC
RDW
Abbreviation
WBC
Neutrophil Concentration
NEU
Neutrophil Percentage
%N
Lymphocyte Concentration
LYM
Lymphocyte Percentage
%L
Monocyte Concentration
MONO
Monocyte Percentage
%M
Eosinophil Concentration
EOS
1-9
Abbreviation
Eosinophil Percentage
%E
Basophil Concentration
BASO
Basophil Percentage
Table 1-4
Abbreviation
Platelet Concentration
PLT
MPV
Platelet-crit
PCT*
PDW*
Table 1-5
%B
Platelet Parameters
Full Name
CS Model
SL Model
Graphics
Printer
Height
Width
Depth
Weight
Table 1-6
SL Model
Graphics
Printer
Height
38 (96.5 cm)
38 (96.5 cm)
12 (30.5 cm)
Width
34 (86.3 cm)
34 (86.3 cm)
Depth
31 (78.7 cm)
31 (78.7 cm)
Weight
1 - 10
Analyzer
Analyzer &
Sample
Loader
Monitor
Printer
Table 1-8
Voltage
Frequency
Max
Current
110 VAC + 10 %
50/60 3 Hz
6 amps
120 VAC + 10 %
50/60 3 Hz
6 amps
220 VAC + 10 %
50/60 3 Hz
3 amps
240 VAC + 10 %
50/60 3 Hz
3 amps
110 VAC + 10 %
50/60 3 Hz
120 VAC + 10 %
Performance Specifications
Linear
Range
WOC
0.10K/L
2.7%
0.99
0.0-250 K/L
NOC
0.10 K/L
2.7%
0.99
0.0-250 K/L
RBC
0.20 M/L
1.5%
0.98
0.0-8.0 M/L
6 amps
HGB
0.1g/dl
1.0%
0.98
0.0-25.0g/dL
50/60 3 Hz
6 amps
MCV
N/A
1.0%
0.98
35-180 fL
220 VAC + 10 %
50/60 3 Hz
3 amps
50/60 3 Hz
RDW
N/A
5.0%
0.91
N/A
240 VAC + 10 %
3 amps
50/60
1.3 amps
PLT
5.0K/L
4.0%
0.98
0.0 - 1750
K/L
50/60
0.5 amps
MPV
N/A
5.0%
0.72
2.0-18.0 fL
1 - 11
Operational Specifications
Operating Environment
13 mm
75 mm
Temperature
Length
Relative Humidity
~ 7 minutes
~ 5 minutes
~ 52 seconds
~ 100 seconds
~ 100 seconds
~ 130 seconds
~ 130 seconds
Open Mode
1 mL
500 L
Batch Size
1 to 50 tubes per batch
Bar Code Formats
Interleaved 2-of-5 code
Codabar
Code 39
Throughput
CS Model
Open
80 Samples/Hour
Closed
68 Samples/Hour
SL Model
Open
80 Samples/Hour
Closed
68 Samples/Hour
125 L
Closed Mode
250 L
1 - 12
TOC
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Table of Contents
2.1
2.2
2.3
Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Troubleshooting Approach . . . . . . . . . . . . . 2-2
Error Messages . . . . . . . . . . . . . . . . . . . . . . . 2-3
IP-1
IP-2
IP-3
IP-4
IP-5
IP-6
IP-7
IP-8
IP-9
IP-10
IP-11
IP-12
IP-13
IP-14
IP-15
IP-16
IP-17
IP-18
IP-19
IP-20
IP-21
IP-22
IP-23
IP-24
2-1
2.1 Summary
Error Messages
Block Diagrams
Normal Scatter/Histograms
Service Commands
2-2
Error
Corrective Action
Corrective Action
IP-27
Leaks, Pressure
IP-18
Leaks, Vacuum
IP-19
IP-13
IP-8
IP-8
IP-27
IP-27
MCH Errors
IP-14
IP-25
MCHC Errors
IP-14
Controls Out
IP-10
MCV Errors
IP-14
Diluent/Sheath Empty
IP-7
IP-24
IP-13
IP-27
IP-27
IP-27
IP-27
IP-27
IP-22
No Tube Present
IP-27
Flags
IP-21
IP-5
Flow Errors
IP-4
Precision Problems
IP-2
Pressure Errors
IP-17
IP-1
Printer Errors
IP-26
HCT Errors
IP-14
IP-27
HGB Errors
IP-13
IP-27
Histogram Errors
IP-9
IP-24
IP-27
Incomplete Aspiration
IP-3
IP-20
2-3
Corrective Action
IP-27
IP-27
IP-27
IP-27
IP-27
IP-27
IP-27
IP-27
IP-27
IP-11
IP-12
Vacuum Errors
IP-17
WBC Imprecision
IP-7
X-B Errors
IP-14
2 - 3A
YES
Probe Leaking
In Ready Mode
Leaking
Stops
Leaking
Stops
YES
NO
YES
- Verify connections on
Ultrasonic Sensor
- Verify and tighten fittings
on Y-Valve
- Verify connection on top
of Probe
- Replace Y-Valve (PL-8)
NO
YES
1
NO
2-4
YES
NO
Liquid
Leaking
?
2-5
Start
NO
YES
Leaking
Stops
Probe Leaking
In Ready Mode
?
Leaking
Stops
YES
NO
YES
- Verify connections on
Ultrasonic Sensor
- Verify and tighten fittings
on Y-Valve
- Verify connection on top
of Probe
- Replace Y-Valve (PL-8)
NO
YES
1
CELL-DYN 3200 SYSTEM SERVICE MANUAL
NO
2
Part Number 9140300 August 1998
2-6
YES
NO
Liquid
Leaking
?
2-7
Start
NO
- Aspirate Normal
Control in both open
and closed modes.
YES
Rotating
Properly
Problem
Corrected
?
YES
Mode(s)
Affected
?
Open Only
Both
NO
Closed Only
- Replace Y-Valve Assy (PL-8) .
- Replace MDM (Pl-9.2)
Total Call
2-8
MORE
TOWER TEST
NEEDLE DOWN
?
NO
NO
- Clean Closed Probe (VP-51).
- Clean Open Probe (VP-50).
- Ensure there are no
restrictions between Open
Probe and Y-Valve.
- Replace Open Probe (PL-8).
NO
Air Gap
Present
?
YES
3
2-9
- Re-seat Ultrasonic
Sensor Cable.
- Replace Ultrasonic
Sensor (PL-8).
- Replace Ultrasonic
Sensor Board (PL-9.2).
- Replace Sample
Sensor (PL-9.1).
- Replace Sample
Sensor Board (PL-9.2).
YES
NO
2 - 10
Start
From Main Menu:
YES
- Run a Count Cycle and observe
the Delivery Syringe and WC1.
Verify that when the syringe is
moving up, liquid is dripping into
WC1 at a fast and constant rate
(See Figures).
DIAGNOSTICS
MORE
MOTOR OPERATION
EXERCISE MOTORS
WC1
68
Delivery Syringe
Dripping
OK
?
NO
Smooth
Movement
?
YES
NO
2 - 11
Output
Stable
YES
NO
2 - 12
Start
- Check resistance of
Overpressure Sensor
Sensor
Shorted
?
MORE
MOTOR OPERATION
SHEAR VAL DISPENSE
NO
YES
- Replace Sensor (PL-4.2)
Problem
Corrected
Times
OK
YES
?
Total Call
?
NO
YES
Total Call
2 - 13
If there is movement:
- Replace defective section:
Front (PL-9.1)
Rear (PL-9.1)
If problem persists:
- Replace Shear Valve Driver
(PL-9.1)
Rim Notch
Faces Right
Total Call
32002
Total Call
2 - 14
Note: This procedure assumes that HGB Results are OK in Open and Closed Modes, and there are no hardware or software Error Messages.
It is designed to find and correct gross WBC, NOC, RBC, and PLT problems. For Optical Precision Problems see IP-15.
Start
Backgrounds
OK
NO
Go to (IP-8),
correct and
return here.
All "0"
YES
NO
NO
YES
YES
YES
?
YES
NO
NO
NO
YES
YES
NO
2 - 15
NOC Only
Low
All Low
YES
YES
WBC Only
High
12
NO
NO
YES
15
NO
All High
RBC & PLT
Only Low
YES
YES
NOC Only
High
13
?
NO
16
10
YES
NO
NO
NO
RBC & PLT
Only High
WBC Only
Low
YES
11
YES
14
2 - 16
All Erratic
YES
WBC Only
Erratic
17
NO
NO
YES
18
NO
NOC Only
Erratic
YES
YES
19
20
?
NO
Total Call
2 - 17
3
- Verify beam alignment
(VP-20), (VP-21).
YES
Power OK
?
NO
NO
- Connect new Laser Head to
Laser Power Supply and
verify power (see Warning).
If good, replace Laser Head
(PL-10). If not, replace Laser
Power Supply (PL-2).
Problem
Corrected
?
YES
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'DQJHU
RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#WKH#ODVHU#FRQQHFWRU#
ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#PDOH#SLQV1#6KRUW#PDOH#SLQV#
WR#&KDVVLV1
Total Call
2 - 18
F1
SET POINT ENTRY
- Verify WBC Threshold is
360.
DIAGNOSTICS
F1
F12
2 - 19
9
- Verify laser power (VP-46).
- Clean Laser Bench (VP-43).
-- Verify Sheath Reservoir is filling
properly.
YES
Power OK
NO
Problem
Corrected
?
YES
?
NO
NO
'DQJHU=#+LJK#9ROWDJH#00#$VVXUH#WKH#SRZHU#WR#WKH#LQVWUXPHQW#
'DQJHU
LV#RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#WKH#ODVHU#
FRQQHFWRU#ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#PDOH#SLQV1#
6KRUW#PDOH#SLQV#WR#&KDVVLV1
Total Call
2 - 20
10
Problem
Corrected
?
DIAGNOSTICS
F12
F1
SET POINT ENTRY
NO
YES
Problem
Corrected
?
Total Call
YES
Total Call
2 - 21
12
13
14
DIAGNOSTICS
F1
F12
F1
- Verify RBC/PLT Threshold is
550.
- Verify 0 o and 10 o Log Amplifiers
(VP-28).
- Verify 0 o and 10 o Gain (VP-26),
(VP-27).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).
2 - 22
15
16
DIAGNOSTICS
F12
Problem
Corrected
?
DIAGNOSTICS
F12
F1
NO
F1
SET POINT ENTRY
- Verify NOC Threshold is
400.
- Perform NOC Gain
Adjustment (VP-25).
YES
Total Call
2 - 23
17
YES
NO
Power Stable
NO
Problem
Corrected
?
NO
'DQJHU=#+LJK#9ROWDJH#00#$VVXUH#WKH#SRZHU#WR#WKH#
'DQJHU
LQVWUXPHQW#LV#RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#
WKH#ODVHU#FRQQHFWRU#ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#
PDOH#SLQV1#6KRUW#PDOH#SLQV#WR#&KDVVLV1
YES
Total Call
2 - 24
18
- From Main Menu,
press
DIAGNOSTICS
F12
F12
F1
F1
NO
- Verify RBC/PLT
Threshold is 550.
19
NO
- Verify operation of WBC
Syringe (VP-6), (VP-7).
?
YES
Total Call
Problem
Corrected
?
YES
Total Call
2 - 25
Start
HGB Lyse
Empty
?
NO
YES
YES
WBC Lyse
Empty
?
NO
YES
?
NO
Total Call
2 - 26
YES
Syringe
Filling
Properly
?
RUN
CLEAR FAULT
Syringe
Moving
Properly
?
NO
YES
- Remove Top Cover and
observe Reagent Sensor
Board. See Figure
- Inspect and seat
connectors on J1 of
Reagent Sensor Board
and J26 of FCM.
- Replace FCM (PL-14.1).
NO
32098
2 - 27
Diluent
Not Filling
Properly
?
YES
NO
NO
Both
Filling
Properly
?
YES
Sheath
Not Filling
Properly
?
NO
YES
NO
Neither
Filling
Properly
?
YES
Diluent Filling
Past Electrode
?
YES
YES
NO
Fill
To
H e re
Sheath Filling
Past Electrode
32099
2 - 28
2 - 29
32100
2 - 30
DIAGNOSTICS
NO
F12
F1
SPECIAL PROTOCOLS
MORE
YES
NO
REAGENT RESERVOIR
- Follow on-screen instructions and press
EMPTY DIL/SHEATH
- From Main Menu, press
DIAGNOSTICS
FILL DIL/SHEATH
F12
F1
SET POINT ENTRY
- Verify WBC Threshold is 360.
- Replace WBC Lyse Container.
- Clean/replace WBC Syringe (PL-7).
Backgrounds
OK
YES
Total Call
2 - 31
Start
- Perform the following in order
and verify histograms after
each action taken.
WBC Only
NO
Backgrounds
OK
NO
Go to
(IP-8) N O
NO
YES
YES
Problem
Corrected
?
YES
Total Call
Problem
Corrected
?
YES
Total Call
2 - 32
Start
NO
Q.C. OK
YES
?
Total Call
- Perform Autoclean.
- From Main Menu, press
SPECIAL PROTOCOLS
MORE
AUTOCLEAN
- Verify Backgrounds (VP-56).
YES
All
Backgrounds
OK
?
NO
Results
Acceptable
NO
?
YES
Total Call
Go to (IP-8).
2 - 33
NO
- Clean Shear Valve (VP-48).
Precision
In Spec
?
YES
- Recalibrate out-ofrange parameter(s)
(VP-58).
- Determine which
parameter(s) are out-ofrange, and go to (IP-15)
or (IP-16) for HGB and
correct.
- For indices (MCH or
MCHC), determine which
parameter (HGB, MCV,
or RBC is imprecise and
troubleshoot accordingly.
MCH = HGB X10
RBC
2 - 34
Start
- Reinitialize Analyzer
From Main Menu:
Liquid Exiting
Analyzer
YES
DIAGNOSTICS
MORE
INITIALIZATION
NO
- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)
Press:
MORE
- Observe the Waste Line into
External Waste Container and
press:
DRAIN ACCUMULATORS
Problem
Corrected
NO
?
YES
- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)
Total Call
2 - 35
1
- Check Cable and replace
if necessary (PL-5)
- Remove connector from
J11 on VPM and check
resistance between
connector pins. Must be
greater than 20
Megohms. See Figure
- Replace Accumulator
(PL-5)
NO
J10
Vac 1 Acc
J11
Vac 2 Acc
VPM
BD
> 20 Megohms
?
YES
32003
2 - 36
Start
- Reinitialize Analyzer
From Main Menu:
Liquid Exiting
Analyzer
YES
DIAGNOSTICS
MORE
INITIALIZATION
NO
- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)
Press:
MORE
- Observe the Waste Line into
External Waste Container and
press:
DRAIN ACCUMULATORS
Problem
Corrected
NO
?
YES
- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)
Total Call
2 - 37
1
- Check Cable and replace
if necessary (PL-5)
- Remove connector from
J11 on VPM and check
resistance between
connector pins. Must be
greater than 20
Megohms. See Figure
- Replace Accumulator
(PL-5)
NO
J10
Vac 1 Acc
J11
Vac 2 Acc
VPM
BD
> 20 Megohms
?
YES
32003
2 - 38
Start
HGB Only
Both
2 - 39
Total Call
Select path from Chart below.
Reference
Sample
2
1950-2050
1950-2050
< 1950
< 1950
> 2050
>2050
Erratic
1950-2050
1600
1950-2050
Erratic
Erratic
5
- Verify tubing, operation (VP-10),
and flow through Valve 94 and
replace as necessary (TM-1),
(PL-4.1).
2 - 40
Start
X-B Parameter
RBC
RBC
MCV
NA
NA
MCH
NA
MCV
HGB
HGB
NA
NA
NA
MCHC
MCV
Controls at or
Near 2 SD
of mean
?
NO
YES
Go to (IP-10)
Controls Out.
2 - 41
1
- Verify Reagent, Type and
Expiration Date.
Shift
After
Maintenance
?
Reagent
Change
Required
?
YES
- Repeat Maintenance
Procedures
performed before X-B
Shift.
- Monitor X-B for 5 Data
Points (100 Samples).
NO
X-B In
Range
YES
- Monitor X-B for 5
Data Points (100
Samples).
?
YES
X-B In
Range
Total Call
NO
?
YES
Total Call
2 - 42
All
Parameters
OK
?
NO
- Determine which
parameter(s) are out-ofrange, and go to (IP-15) or
(IP-16) for HGB and correct.
YES
- Verify Calibrator Insert
Sheet matches Current
Calibrators.
- Perform System
Calibration (VP-58) .
2 - 43
Start
- Verify Background Counts
(VP-56).
Go to (IP-8).
YES
YES
All
Imprecise
NO
Problem
Corrected
?
RBC/PLT
Only
?
YES
NO
YES
MORE
AUTOCLEAN
?
NO
Q.C. SPECIMEN
SPECIAL PROTOCOLS
Backgrounds
OK
RUN
NO
1
2 - 44
2
- Replace WBC Lyse Container.
YES
F12
F1
SET POINT ENTRY
WBC
Only
?
NO
NOC
Only
?
YES
- Ensure HGB Mixing
Chamber is being rinsed
and drained properly.
NO
Total Call
2 - 45
2 - 46
Start
HGB CVs
OK
?
YES
NO
Total Call
NO
- Replace HGB Lyse
Container>
NO
HGB CVs
OK
?
YES
2 - 47
5
4
YES
3
NO
Vac
Constantly
Running
NO
- Reinitialize Analyzer
9P SI
YES
12P SI
TO
FLO W PA N E L
To S L
Pres
Constantly
Running
To S L
Start
3 2 00 4
NO
Both
Constantly
Running
?
YES
32005
2 - 48
1
All Valves
Close Then
Open
?
Both LEDs
ON
NO
2
+12 VNR
Fuse (Top)
YES
NO
- Replace Pump Relay
Board (PL-5)
32006
YES
NO
- Reinitialize Analyzer
From Main Menu:
DIAGNOSTICS
MORE
Problem
Corrected
Fuse
Blown
INITIALIZATION
- Observe Flow Panel
valves
NO
YES
Total Call
YES
2 - 49
2
Note: If the +24 UR is approximately
the same as the +12 UR, the +24V
Fuse is blown or the +24V Power
Supply is defective.
- Replace Fuse if
necessary (PL-1)
NO
NO
YES
Fuse
Blown
YES
?
Total Call
YES
NO
32007
2 - 50
TO
FL O W PA N E L
P inch
Pumps
Stop
?
NO
12P SI
9P SI
YES
- Unpinch tubing
To S L
To S L
32008
Pumps
Stop
NO
?
- Inspect 12 psi and 12" Hg
plumbing and
Accumulators. Replace
as necessary (PL-5),
(TM-2)
- Inspect Vent Valves 1 and
4. Replace as necessary
(PL-5)
- Replace VPM (PL-5)
YES
2 - 51
4
Pump
Stops
YES
Pump
Stops
?
YES
To S L
To S L
9P S I
P inch
1
TO
FL O W PA N E L
NO
YES
12P S I
LED
ON
NO
32 0 09
2 - 52
5
Pump
Stops
YES
Pump
Stops
?
To S L
To S L
9P SI
Pinch
TO
FLO W PAN E L
YES
YES
5P SI 12"H g 8"H g 12P SI 12"H g
NO
12P SI
LED
ON
NO
32010
2 - 53
- Replace VPM
(PL-5)
NO
YES
LEDs
ON
- Replace VPM
(PL-5)
NO
NO
YES
NO
Pres
Doesn't
Run
VAC LED
ON
NO
VAC
Doesn't
Run
YES
- Replace VPM
(PL-5)
- Reseat connectors VPM J18,
PRM J1, J2
YES
Pres LED
ON
?
YES
2 - 54
7
NO
Both
OK
- Re-connect tubing to
positions 6 and 7 (6-Red,
7-Green)
Pump Head:
Pressure (PL-5)
YES
Vacuum
(PL-5)
Pump Assy:
- Perform Vacuum and
Pressure Level
Verification/Adjustment
(VP-16)
Pres
Cycles
< 2 min
YES
Pressure (PL-5)
Vacuum
NO
Vac
Cycles
< 2 min
(PL-5)
NO
Total Call
See Pressure Leaks (IP-18)
YES
2 - 55
To
C D M 2-J3
FC M -J5
To
S D M /C D M -J3
J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5
B a ck
C D M 2 & S D M /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
5
1
Fro m
+1 2 V U R
To
4
2
P.S .
SDM A
UR RET
J1/J2 3
3
1
PSM
J2
1
2
3
J3
To
S h ea r
Valve
D rive r-J3
+2 4 V U R
1
2 RET
N o te: J4 an d J5 a re no t o n
S D M /C D M
To
S D M 9 -J1 0
J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET
J4
1
2
To
SDM C
3
FC M
J5
1
2
3 2 0 11
To
SDM B
To
SDM D
2 - 56
- Reinitialize Analyzer
From Main Menu:
DIAGNOSTICS
MORE
Pump
Cycles
< 2 min
YES
INITIALIZATION
NO
Total Call
- Adjust Pressure Levels if
necessary (VP-16)
2 - 57
1
- Check +24V UR (28.9V37.7V) and Fuse
(Bottom Fuse)
- While observing VPM
Solenoid LEDs (See
Figure) press:
INITIALIZATION
- Replace Fuse if
necessary (PL-1)
NO
NO
- Check Fuse again
Problem
Corrected
LEDs Bright
Then Dim When
Activated
YES
Fuse
Blown
YES
?
Total Call
YES
NO
3 2 0 07
2 - 58
2 - 59
Pres
3
OK
?
NO
NO
To S L
YES
Pres
1
OK
?
9P SI
NO
12P SI
To S L
TO
FL O W PA N E L
Pres
2
OK
Pres
3
OK
YES
NO
32004
YES
Total Call
2 - 60
YES
P inch
NO
12P SI
9P SI
NO
TO
FL O W PA N E L
To S L
To S L
Pres
2
OK
Pres
1
OK
32009
2 - 61
6
- Inspect plumbing to
Valves 32, 98, and 18.
- Unpinch tubing and pinch
at position 6 on Manifold.
Pres
1
OK
?
YES
2 - 62
To
C D M 2-J3
FC M -J5
To
S D M /C D M -J3
J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5
B a ck
C D M 2 & S D M /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
1
5
Fro m
+1 2 V U R
To
4
2
P.S .
SDM A
UR RET
J1/J2 3
3
1
PSM
J2
1
2
3
J3
To
S h ea r
Valve
D rive r-J3
+2 4 V U R
1
2 RET
N o te: J4 an d J5 a re no t o n
S D M /C D M
To
S D M 9 -J1 0
J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET
J4
1
2
To
SDM C
3
FC M
J5
1
2
3 2 0 11
To
SDM B
To
SDM D
2 - 63
- Reinitialize Analyzer
From Main Menu:
DIAGNOSTICS
MORE
Pump
Cycles
< 2 min
YES
INITIALIZATION
NO
Total Call
- Adjust Vacuum Levels if
necessary (VP-16)
2 - 64
1
- Check +24V UR (28.9V37.7V) and Fuse
(Bottom Fuse)
- While observing VPM
Solenoid LEDs (See
Figure) press:
INITIALIZATION
- Replace Fuse if
necessary (PL-1)
NO
NO
- Check Fuse again
Problem
Corrected
LEDs Bright
Then Dim When
Activated
YES
Fuse
Blown
YES
?
Total Call
YES
NO
5
3 2 0 07
2 - 65
2 - 66
NO
Vac
2
OK
Vac
2
OK
YES
- Inspect Vac 2
Accumulator and
plumbing on Air Supply.
Replace as necessary
(PL-5), (TM-2)
5
4
3
12P SI
9P SI
Total Call
YES
TO
FL O W PA N E L
To S L
NO
To S L
NO
?
Vac
1
OK
32004
2 - 67
4
- Unpinch tubing and
pinch at position 7 on
Manifold.
- Inspect plumbing to
Flow Panel.
NO
Vac
1
OK
Vac
1
OK
9P SI
12P SI
YES
TO
FL O W PA N E L
P inch
- Inspect 12" Hg
plumbing and
Accumulator. Replace
as necessary (PL-5),
(TM-2)
To S L
NO
?
To S L
- Inspect plumbing to
Valves 31 and 97.
32010
2 - 68
To
C D M 2-J3
FC M -J5
To
S D M /C D M -J3
To
S h ea r
Valve
D rive r-J3
J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5
B a ck
C D M 2 & S D M /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
5
1
Fro m
To
+1 2 V U R
4
2
P.S .
SDM A
UR RET
J1/J2 3
3
1
PSM
J2
1
2
3
J3
+2 4 V U R
1
2 RET
N o te: J4 an d J5 a re no t o n
S D M /C D M
To
S D M 9 -J1 0
J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET
J4
1
2
To
SDM C
3
FC M
J5
1
2
3 2 0 11
To
SDM B
To
SDM D
2 - 69
Start
- Check +24V
Fuse (Bottom
Fuse).
NO
- Replace Fuse if
necessary (PL-1).
28.9V-37.7V
?
From Main Menu, press
28.9V-37.7V
NO
DIAGNOSTICS
MORE
INITIALIZATION
YES
YES
- Verify +24V between Pins 1
and 2 of Shear Valve Driver
Board. If not present re-seat
cable.
- Re-seat cable from FCM-J8
to SVDM-J2.
YES
Total Call
NO
Fuse
Blown
Total Call
YES
?
NO
2 - 70
To
C D M 2-J3
FC M -J5
To
S D M /C D M -J3
J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5
B a ck
C D M 2 & S DM /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
5
1
Fro m
+1 2 V U R
To
2
P.S . 4
SDM A
UR RET
J1/J2 3
3
1
PSM
J2
1
2
3
J3
To
S h ea r
Valve
D rive r-J3
1
2
+2 4 V U R
RET
N o te: J4 and J5 a re no t o n
S D M /C D M
To
S D M 9 -J1 0
J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET
J4
1
2
To
SDM C
3
FC M
J5
1
2
3 2 0 11
To
SDM B
To
SDM D
2 - 71
Start
Problem
Corrected
YES
?
Total Call
NO
- Perform Autoclean
MORE
- Verify Background
Counts (VP-56).
YES
AUTOCLEAN
- Clean Shear Valve (VP-48).
- Manually clean, debubble, and wet
Optical Flow Cell (VP-44), (VP45).
NO
Backgrounds
OK
YES
Problem
Corrected
?
Total Call
2 - 72
Flagging
Parameters
All Flagging
DIAGNOSTICS
DIAGNOSTICS
F12
F12
F1
F1
Var Lym
LRI
Band
URI
IG
LURI
RBC Flags
RBC Morph
Blast
2 - 73
Start
System Uses
Waste Sensor Cable
NO
Problem
Corrected
YES
Total Call
NO
- Insert Insulated Tool into
Sensor Jack to open
contacts. See Figure
NO
Problem
Corrected
?
Total Call
YES
2 - 74
Problem
Corrected
YES
NO
Problem
Corrected
YES
Total Call
NO
2 - 75
- Verify Laptop
information displays on
Monitor.
NO
- Valves activate.
- 7-segment LED increments
until "E" and "1" are alternately
displayed.
Information
Displays
YES
Problem
Corrected
YES
Total Call
YES
Data Station CRT
NO
Initialization
OK
?
- Replace Monitor (PL17.3).
NO
- Troubleshoot
"dead" Analyzer
problem.
32101
2 - 76
Start
- In order replace the
following.
NO
Problem
Corrected
?
YES
Total Call
2 - 77
Start
- Format the Hard Drive (VP-61).
- Install Software (VP-53).
- Load System Setup (VP-65), (VP63).
NO
Problem
Corrected
NO
?
Problem
Corrected
YES
?
Total Call
YES
Total Call
2 - 78
Start
Is Printer
Working
NO
?
Clear Printer buffer.
NO
YES
Printer
On-Line
Printer
On-Line
NO
YES
- Replace Printer.
SETUP
CUSTOMIZE
REPORT
Total Call
STOP PRINTING
CONFIRM STOP
Printer
Reset &
Print
?
NO
STOP PRINTING
CONFIRM STOP
YES
Total Call
2 - 79
1
- Connect Laptop Printer to
Printer Connector to
verify problem.
NO
NO
Prints
OK
- Replace Motherboard
(PL-17.2).
Laptop
Printer
Prints
?
?
YES
YES
- Replace Printer.
- Replace Printer Cable
(PL-17.3).
2 - 80
Associated VP(s)
Associated Components
Parts List(s)
VP-36
PL-18.2, PL-18.1
VP-36
PL-18.2, PL-18.1
VP-35
PL-18.1, PL-18.2,
PL-18.3
VP-35
PL-18.1, PL-18.2,
PL-18.3
VP-.39
Sensor, SHM 2
PL-18.2, PL-18.3
VP-39
Sensor, SHM 2
PL-18.2, PL-18.3
VP-39
PL-18.2, PL-18.3
VP-38
PL-18.2, PL-18.3
VP-31
PL-6.1, PL-8
VP-31
PL-6.1, PL-8
VP-34
PL-6.1, PL-8
PL-18.2, PL-18.3
PL-18.1, PL-18.2,
PL-18.3
PL-18.1, PL-18.3
VP-38, VP-39
VP-37
VP-38
VP-38
VP-38
VP-40
Sensor, SHM 2
PL-18.1, PL-18.2
PL-18.1, PL-18.2
2 - 81
Associated VP(s)
VP-40
VP-33, VP-11
Associated Components
Parts List(s)
2 - 82
$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP
Table of Contents
Figure 2A-1
2A - 1
$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP
Figure 2A-1 Cable Connection Diagram, SL
9601320
FCM
SOL
1
2
3
4
5
(SOL 11-18)
SDM 1
SOL
9600930
NC
J1
J6
7
J2
J7
J3
J8
8
J4
J9
9520830
J5
J10
1
2
3
4
5
9520830
J56
J57
J58
J3
J1
SPEAKER &
STATUS BD
J2
SP
P/O
SDM/CDM
(SOL 41)
J61
J68
J67
J2
3
4
J3
J4
J15
J17
J5
J12
12"VAC OVFL
9520528
8"VAC OVFL
9520833
TEST CONN.
9520879
PRES PUMP
VAC PUMP
1
2
3
4
5
SDM 5
9600930
J6
J1
6
J2
J7
7
J3
J8
NC
J4
J9
9520859
J5
J10
9520860
NC
NC
9520851
J23
J24
9520861
9520866
9520849
PERIPHERAL BUS
J2
J5
J11
J10
(SOL 61-68)
1
2
3
4
5
SDM 6
9600930
J6
J1
6
J2
J7
7
J3
J8
8
J4
J9
J5
J10 9320860
1
2
3
4
5
J18
J8
9520385
HGB
J2
J3
NC
LASER
9520869
LASER P/S
9520896
J4
J15
NC
NC
J5
J3
9520853
J10
NC
NC
J7
J6
J12
J6
J7
9520001
FAN
J3
J8
9520620
FAN
J4
J13
9520829
J8
J1
J7
J13
J14
9520002
PWR SW
J9
J4
9520861
9520897
9520898
J6
9520789
J6
J7
J2
9520782
9520865
9520543
9520845
9520844
J2
MOTOR
A CORE SYR.
J1
J2
MOTOR
E PROBE
J1
J2
MOTOR
G SAMPLE PUMP
J1
9520155
9520842
J2
J10
NC
9520894
J3
J12
NC
J13
NC
NC
J2
J13
J5
J14
9520892
1
SOLENOID
CHAIN CABLE
CHAIN CABLE
J1
J2
MOTOR
C WBC LYSE SYR.
J1
J2
MOTOR
D RBC/HGB DIL SYR.
CHOPPER DRIVER
3X 9600420
J1
TO SL VAL-1
J2
TO SL VAL-2
J3
TO SL VAL-3
J4
TO SL VAL-4
J5
TO SL VAL-5
J10
TO SL MIXER SENS.
J12
9520883
9520882
9520780
TO TOWER MOTOR
J13
TO TOWER DC-MOTOR
J15
TO COVER SENSOR
J9
J11
J16
ASPIRATION
TOWER
CONTROLER
Y-VALVE ASSY
BOTTOM VIEW
MOTOR
B HGB LYSE SYR.
9601910
SHM(2)
J12
TO PC MOTHERBOARD
J2
NC
9601910
SHM(1)
9520884
J1
TO TOWER SOL.
9520872 TO TOWER SENS.
J10
9520894
J1
9520154
9520563
J14
9520895
9520612
PMT (CH4)
9520847
9520895
SDM1-4.....FLOW PANEL
SDM5,6.....REAGENT PANEL
J1
J2
J3
PMT PREAMP
9601010
9520856
J9
J11
+/-15V
32054
*S/L=SERIAL LINK
9520855
J8
NC
J9
9520871
9601000
PREAMP 10 DEG (CH2)
PMT PREAMP
9601010
J1
PMT (CH3)
J2
J3
J11
J12
J1
9520543
9601000
PREAMP 0 DEG (CH1)
J1
J10
J13
MOTOR
J4
J5
J3
J4
J1
MPM
9600920
J501
9520896
SPARE
9520782
9520854
J11
NC
J2
J1
J5
J7
J4
9520506
MAM
9601950
J2
BACK
9520869
9520866
9520828
J6
J3
NC
ADC2
J3
9520866
9520871
J1
J1
J5
9520841
TO REAGENT
RESERVOIRS
MDM 9601940
J2
9520789
9520841
TOP
9212064
FLOPPY
9520795
9520848
NC
9340008
LINEAR P/S
9212057
NC
HARD DISK
9520796
9520846
J5
J7
J6
J2
J1
PDM
9601980
(SOL 91-98)
SDM 9
9600930
J1
J6
J2
J7
J3
J8
J4
J9
J5
J10
NC
9520893
J4
J1
9520865
9520878
DIL SENS
J8
NC 9520835
J9
SHEATH SENS
J10
9520836
J3
J9
SPM
9601830
9520865
J3
9520892
DILUENT
9520839
P OVER PRESS
SENSOR
EX WASTE
9520857
ADC3
J4
9520878
CDM 2
9601080
J7
J4
DAC3
J13
J16
9520850
J17
J3
9520841
J19
9520864
9520865
9520500
J3
9520846
J11
9520505
J10
J12
J1
J2
J6 J18
J1
J2 PUMP
J4
J3 RELAY
9601200
J8
J7
NC
J20
J5
9520862
J1
DAC1
ADC1
9520852
ULTRA SONIC
SAMPLE DET
J4
J11
J16
J6
J14
J21
9520831
9520832
J10
9520870
J11
9520829
9520831
J13
9520832
J14
J16
J5
9600910 IN-LINE
REAGENT
SENSOR BD
J14
J1
9601161
2nd SAMPLE DET.
9520841
9520849
9520841
9520849
9520829
J6
J15
9520862
9520826
9520859
J22
J2
9520820
J1
9520879
(SOL 51-58)
J7
J25
VAC/PRES
9601220
START SW
J13
J26
9520838
9520834
1
J1
J12
9520826
NC
SOLENOID
6
7
9520828
J9
CPU
9601820
J6
9520825
NC
9520825
9520847
9520783
+5V. +/-15V
EXT. CPU BUS
2
3
J51
J52
J53
J54
J6
P/O
SDM/CDM
(SOL 31-38)
J3
NC
J9
9520858
(SOL 21-28)
SDM 2
9600930
J1
J6
6
J2
J7
7
J3
J8
8
J4
J9
9520858
J5
J10
9520837
9520893
9520890
9520888
9520887
9212106
TO SL MIXER MOTOR
TO BAR CODE READER
TO SL RACK SENSORS
TO SL TUBE SENS ASSY
J16
SAMPLE LOADER
CONTROLLER
4PLCS
9520255 ASY3A
2A - 2
$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV
Table of Contents
Figure 2B-1
Figure 2B-2
Figure 2B-3
Figure 2B-4
Figure 2B-5
Figure 2B-6
Figure 2B-7
Figure 2B-8
Figure 2B-9
Figure 2B-10
Figure 2B-11
Figure 2B-12
Figure 2B-13
Figure 2B-14
Figure 2B-15
Figure 2B-16
Figure 2B-17
Figure 2B-18
Figure 2B-19
Figure 2B-20
2B - 1
Display
PC Module
VPM
High Speed
Serial Link
Tag Bus
SPM
High
Speed
External
CPU
Bus
FCM
MAM
CPU/DCM
32043
2B - 2
Display
PC Module
High Speed
Serial Link
ADC1
A/D
MUX
ADC3
ADC2
Tag Bus
CPU/DCM
ADC4
MAM
SPM
FCM
VPM
32044
2B - 3
Display
PC Module
High Speed
Serial Link
DAC1
D/A
DAC2
DAC3
CPU/DCM
DAC4
MAM
SPM
FCM
VPM
32045
2B - 4
Display
PD
0 o PAM
0 o Lin
10 o Lin
PD
10 o PAM
90 o Lin
90 o D Lin
PMT
90 o PAM
MAM
PC Module
0 o Log
10 o Log
High Speed
Serial Link
SPM
Analog Out
VDYN
Tag Bus
PMT
90 o D PAM
CPU/DCM
VDYN
External CPU Bus
HGB
Flow
Cell
Peripheral Bus
LED Current
HGB Signal
HGB Voltage
FCM
32046
2B - 5
Bus
3-1 WC2 Vacuum Supply (5" Hg)
3-2 WC2 Pressure (12 psi)
3-3 RBC Diluent Syringe Supply
3-4 CS Probe Diluent Flush (NC)
CLK
(CDM1)
Motor
Drive
Module
DC
Y-Valve
Motor
Flush
Peripheral Bus
32047
2B - 6
FCM
CLK
SDM 5
5-1
5-2
5-3
5-4
5-5
5-6
5-7
5-8
SDM 6
6-1
6-2
6-3
6-4
6-5
6-6
6-7
6-8
SDM 9
9-1
9-2
9-3
9-4
9-5
9-6
9-7
9-8
CDM 2
Peripheral
Bus
CPU/
DCM
CLK
CLK
32048
2B - 7
A
SHM1
DC Barcode Spin
RS-485
Serial
Bus
MPM
Serial
Bus
MPM
CPU/DCM
Driver
Motor
AC Vacuum Pump
Peripheral Bus
VPM
AC Pressure Pump
FCM
4-7
SCM/CDM
4-8
ON/OFF
CW/CCW
Motor
Drive
Module
DC
Y-Valve
Motor
32049
2B - 8
Display
Vacuum Accumulator #1 Wet
VPM
Vacuum Accumulator #2 Wet
PC Module
High Speed
Serial Link
Diluent Empty
WBC Lyse Empty
HGB Lyse Empty
CPU/DCM
Peripheral Bus
RS-485
Serial
Bus
FCM
Tube Height (S1)
Tube Height (S2)
SHM1
Shear
Valve
Driver
GS1 Home
Door Closed
Sample Cover
Interface
Board
Interface
Board
MDM
32050
2B - 9
RS-485 Bus
To CPU/DCM
Power
From PDM
Tube
J16
SHM2
J12
J9
32105
2B - 10
Vacuum/Pressure Module
32051
ADC4
Analog Multiplexer
Multiplexer
&
Sample and Hold Circuitry
Threshold Setpoints
DAC4
P1
SPP1
Pres #1
Regulator
12 psi
P2
SPP2
P1 P2 P3 V1 V2
P3
V1
SPP3
Pres #2
Regulator
9 psi
V2
SPV1
Pres #3
Regulator
5 psi
SPV2
Vac #1
Regulator
12" Hg
V3
Vac #2
Regulator
(Variable)
V5
Relay PCB
Relay PCB
V2
Pressure
Pump
Pres #1
Accumulator
12 psi
Pres #2
Accumulator
9 psi
Pres #3
Accumulator
5 psi
Vacuum
Pump
V1
V4
Vent
Vent
Vac #1
Accumulator
12" Hg
Vac #2
Accumulator
5" Hg
(Varies)
2B - 11
PDM J2
J3
J13
+ 5, GND-PC MB
+ 12, -12, GND, PF-PC MB
+ 5, -15, GND-MAM, SPM, CPU
J4
+ 5, + 24, GND-MDM
+ 5V
J5
Filter
(CORCOM)
J5
+ 12V
-12V
+ 24V
GND
J6
J7
Spare
+ 5, + 15, -15, GND-VPM, FCM
J8
Fuse
J4
(Rear)
+ 15V
-15V
J10
J9
GND
AC Voltage
Selector
J11
J12
J13
Transformer
J4
(Top)
115VA C
LASER P/S
+ 2400VDC
LASER
J7
J8
115VAC, PRM
J2
32052
2B - 12
Flo p p y D is k
D r iv e
D ri v e
C PU / D C M
K e y b o ard
HSSL
M o t h er b o a rd
Pa r a lle l/
S e ria l Po rt s
H SS L
C o n t r o ller
Pa r a lle l/
S e ria l Po rt s
Bu s
V id e o D is p la y
C o n t r o ller
32053
M e m b r a n e K e y b o a rd
Po w er
V id e o D is p la y
S u p p ly
M o d u le
C RT
2B - 13
TP1
TP3
J10
6
Amplifiers
CH 1 Lin
CH 2 Lin
CH 3 Lin
CH 4 Lin
CH 1 Log
CH 2 Log
Lin Amplifiers
TP2
0 Photodiode Preamp
SPM
MAM
J1
TP8
2
Log Amplifiers
J15
J6
1
3
5
7
9
11
1
3
5
7
9
11
CH 1 Lin
CH 2 Lin
CH 3 Lin
CH 4 Lin
CH 1 Log
CH 2 Log
J7
Peak
Hold
Circuits
Amplifier
MUX
1
ADC3
2 To CPU/DCM
3
AGND
AGND
TP1
Amplifiers
AGND
J1
TP4
J12
6
Lin Amplifiers
FCM
TP2
10 Photodiode Preamp
TP9
2
J18
HGB Voltage
TP7 TP1
Log Amplifiers
HGB
Flow
Cell
AGND
AGND
LED Current
Amplifier
1
2
3
4
5
J25
MUX
Amplifier
1
ADC1
2 To CPU/DCM
3
AGND
TP2
Amplifiers
J2
TP5
J13
4
AGND
AGND
J24
Lin Amplifiers
1
TP3
PMT
VDYN/-100
AGND
Amplifier
8
ADC4
From
VPM
TP1
90 PMT Preamp
2
32055
AGND
TP2
Amplifiers
TP6
J14
4
Lin Amplifiers
TP3
PMT
VDYN/-100
AGND
J2
TP2
TP1
90 D PMT Preamp
2
+10V Reference
AGND
2B - 14
CLK
J1
CLK
CLK
J2
CLK
CLK
Peripheral Bus
J2
J1
ON/OFF
CW/CCW
J6
Y
Valve
Motor
J2
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8
CDM2
J1
J2
1-1
1-2
1-3
1-4
1-5
1-6
1-7
1-8
2-1
2-2
2-3
2-4
2-5
2-6
2-7
2-8
5-1
5-2
5-3
5-4
5-5
5-6
5-7
5-8
J2
1
2
To
3
5 SDMB
6
7
J4
1
2
3
5
6
7
SDM6
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8
J6
SDM1
SDM2
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8
On CLK A
1
Hi CLK A
2
On CLK B
3
Hi CLK B
From 4
On CLK C
FCM 5
Hi CLK C
6 On CLK D
7
Hi CLK D
8
Reset
9
SDM5
DC
1
2
To
3
5 SDMA
6
7
J6
J1
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8
J16
CPU/DCM
J17 J1
+24V UR
5
From 4 +12V UR
P. S. 3 UR RET
1
MDM
J68
J67
J4
J19
3-1
3-2
3-3
3-4
3-6
3-7
3-8
4-1
J6
FCM
J1
J3
J51
J52
J53
J54
J56
J9 J57
J58
J61
J3
CDM Connectors
SDM/CDM
6-1
6-2
6-3
6-4
6-5
6-6
6-7
6-8
9-1
9-2
9-3
9-4
9-5
9-6
9-7
9-8
To
SDMC
J5
1
2
To
3
5 SDMD
6
7
SDM9
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8
32056
VPM
J17
RS-485 Bus
J1
J2
J3
J4
J5
SHM1
Aspiration Tower
J16 J1
J2
J3
J4
J5
J6
J7
J8
GS2 Stop
Door Lock
Spare
Spare
Spare
Spare
Spare
Spare
RS-485 Bus
J16
J1
J2
J3
J4
J5
J6
J7
J8
J1
On CLK 1
Hi CLK 1
On CLK 2
Hi CLK 2
On CLK 3
Hi CLK 3
On CLK 4
Hi CLK 4
Reset
J3
1
2
3
4
To
5
CDM1
6
7
8
9
15
PD0
PD1
PD2
PD3
PD4
PD5
PD6
PD7
+5V
1
2
3
4
To
5
SDMs
6
7
8
9
10
J9
PD0
1
PD1
2
PD2
3
PD3
4
PD4
5
PD5
6
PD6
7
PD7
8
+5V
9
10
+24VUR
Hi/Lo
+12VUR
Driver
TP1
2
AGND
On/Off
SHM2
FCM Connectors
Sample Loader
V1 Rack Advance
V2 Load Side Cross Transfer
V3 Mixer Lift
V4 Mixer Bladders
V5 Unload Side Cross Transfer
Spare
Spare
Spare
Driver
On CLK 5
Hi CLK 5
On CLK 6
Hi CLK 6
Reset
J4
1
2
3
To
4 CDM2
9
15
+24VUR
+12VUR
UR RET
On CLK 9
Hi CLK 9
Reset
J1-J8
DGND
DGND
J2
1
2
To
3
5 SDM9
6
7
J10
+24VUR
1 +12VUR
2 UR RET
3
On CLK
5 Hi CLK
6
Reset
7
On/Off
TP2
Driver
Solenoid Drive
1
2
AGND
2B - 15
J3
JI
JI
CDB
J2
Delivery Syringe
CPU/DCM
J4
JI
CDB
J2
PH0
J5
JI
CDB
J2
PH0
J6
JI
CDB
J2
PH0 1
Motor
MPM
JI
J7
CDB
J2
Open Probe
JI
CDB
J2
J3
Ramping
A/D
Converter
FB-
+5V
M0 PH0
M0 PH1
M0 PH1
J3
J3
TO
CDB
M0
Current
Control
Latch
DC
3
4
TO
CDB
+24V 1
J8
Driver 2
M0 I1
M0 PH0
1
+24V
M0
Phase
Control
Latch
PH1 4
PH1 5
J3-J14
TO
CDB
M0 I0
J1
MPM
Serial
Link
Discharge
SHM2 J13
PH1
PH1
J9
Discharge
Complete
PH0 2
J13
SHM1
J8
Microprocessor
&
Digital Control
Motor
M0 I0
M0 I1
TO
CDB
M1-M11
Control
Latches
J4-J14
FCM
J8
+5V
DGND
4
5
FB+
J2
On/Off
CW/CCW
Shear
Valve
J4
Driver
Module
+ Drive
- Drive
DC
8
FB-
9
10
Y-Valve Motor Control
J1
PH0
J2
6
4
5
TO
MPM
7
2
Stepper
Motor
Phase 0
Driver
I0
I1
MA
PH0 1
MB
PH0 2
Motor
PH1
Stepper
Motor
Phase 1
Driver
+24V
3 +5V
MA
MB
PH1
J67 2
8
9
FB+
FB-
CW/CCW
FB-
DC
2 J1
4
5
GND
Winding
Voltage
Feedback
(Diagnostics)
Y-Valve Motor
1
MDM J14
3+
FB+
2 J2
TP1
1
On/Off
J68 2
SDM/CDM
1
VPM
J18 3
4
+5V
Vac. Pump On
Pres. Pump On
4-
AC In 1
K1
AC Out 2
F1
Line
Neutral J4
115VAC In
Vacuum Pump
1
J1
1
J3
AC
4
3+
AC In 1
K2
4-
AC Out 2
32057
1
J2
2
AC
Pressure Pump
2B - 16
IFSB
Electrode +
FLUID
LINE
Electrode -
E1
Diluent Empty
E2
E4
J1
E5
+5V
Tube Height Sensor (S2)
E6
J26
E8
GND
J9-10
6 J10
9
Cover Sensor
Door Sensor
SHM1
CDM2
1
2
3
4
5
6
7
8
9
10
11
12
In
In
In
In
1 J7
10
10
1 J8
11
11
13
13
J2
J6
Sheath Reservoir Level
1 J10
GND
15
DGND
6 J9
+5V
J11
SHM2
15
J10
Mixer Lifter Up Sensor
J11
TP4
TP2
1
+5V
1
2 J14
Ref
J1 3
AGND
In
25KHZ LED Drive
In
AGND
Vacuum #1 Wet
1 J1
Vacuum #2 Wet
VPM
1 J2
AGND
1
2
3
4
5
6
7
8
9
10
11
12
Y-Valve Sensors
Closed Mode Sensor
2 J4
2 J5
MDM
1
+5V
J1 2
3
2 J11
AGND
+5V
Shear Valve Driver Module
3
4
+5V
Valve CCW
Valve CW
2
3
4
J1
TP1
3
J2
DGND
6
J4-J5
+5V
1
2
3
DGND
Valve CW
In
J8
Valve CCW
AGND
NO
+5V
GND
J10
3
32058
2B - 17
BACK
J501
PWR Fail
PWR Fail
1
2
-12V 3
+12V 4
+24V 5
GND 1 6
GND 1 7
+5V 8
+5V 9
J8
To
FCM-J21
VPM-J12
1
2
3
4
5
6
+5V
GND 2
TP8
+15V
GND 2
-15V
+15V
TP2
-12V
J9
Input
From
P.S.
J4
1
2
3
4
-15V
GND 2
KEY
+15V
TP7
+24V
PWR Fail
+5V
+12V
-12V
GND 1
GND 1
J10
To
PC
Hard
Disk
+12V
1 GND 2
2 GND 2
3 +5V
4
TP1
TP5
J11
To
PC
Floppy
Disk
1
2
3
4
+12V
GND 2
GND 2
+5V
GND 1
GND 2
GND 1
-15V
+24V
1
2 +5V
3
4 +15V
5 -15V
6 GND 2
7 GND 2
8
To
SHM 2-J12
To
PDM-J1
To
CDM2-J3
FCM-J5
J3
1
2
3
4
5
6
1
2
3
4
5
6
7
8
J5
+24V 1
+5V
2
GND 1 3
To PC
Motherboard
To
MAM-J3
SPM-J3
CPU/DCM-J14
To
SDM/CDM-J3
To
Shear
Valve
Driver-J3
To
PDM-J9
+5V
+24V
+5V
+15V
-15V
GND 2
TP3
J6
-12V
+12V
+24V
GND 1
GND 1
GND 1
+5V
1
2
3
4
5
6
7
8
From
P. S.
J1/J2
-12V
+12V
+24V
GND 1
GND 1
+5V
+5V
J2
J1
UR RET
1
3
4 +12V UR
5 +24V UR
1
To
2 SDMB
3
J2
UR RET
1
3
4 +12V UR
5 +24V UR
J4
1
2
3
J5
J4
1 -15V
2 GND 2
3 +15V
1
To
2 SDMD
3
To
PRM-J4
To
MPM-J2
To
Laser
Power
Supply
J7
TP4
+12V
-12V 1
+12V 2
3
GND 1 4
GND 1 5
GND 1 6
7
+5V 8
J2
115VAC
1
2 Neutral
TOP
J4
115VAC
1 Neutral
2
To
Rear
Fan
J7
115VAC
1
2 Neutral
Spare
To
Side
Fan
J8
115VAC
1 Neutral
2
1
+24V
+5V
M0 I0
M0 I1
M0 PH0
M0 PH1
FB+
FB-
To
SDMC
J3
+24V UR
1
2 RET
-15V
GND 2
J3
To
MDM-J3
TP6
J13
1
2
3
4
5
6
7
8
To PC
Motherboard
J4
+5V
+5V
GND 1
GND 1
J12
To
SHM 1-J12
J2
GND 1
1
GND 1 2
+5V 3
4
+5V 5
+5V 6
Input
From
P.S.
J501
1
2
3
4
5
6
7
8
9
J3
To
SDMs-J9
To
SDM9-J10
To
Shear
Valve
Driver-J2
To
Ultrasonic
Sample
Sensor-J3
To
Second
Sample
Sensor-J1
9 +5V
10 GND
J4
+24V UR
1
2 +12V UR
3 UR RET
J8
1 +5V
3 GND
J11
1 +5V
3 GND
J14
1 +5V
3 GND
3
4
5
6
TO
CDB
32059
7
8
9
10
2B - 18
2B - 19
3 rd Cut
M
E
0 Size
90 Dep
B
M
0
B - Basophil
E - Eosinophil
10 Complexity
L - Lymphocyte
M - Monocyte
32103
N - Neutrophil
2B - 20
PLT
PLT
RBC
2. Single Peak
32104
2B - 21
$SSHQGL[#5�#6\VWHP#)ORZ#'LDJUDPV
Table of Contents
Figure 2C-1 Flow Diagram, Complete . . . . . . . . . . . . . . . . . 2
Figure 2C-2 Sample Loader Flow Diagram . . . . . . . . . . . . . 3
Figure 2C-3 Vacuum/Pressure Flow Diagram . . . . . . . . . . . 4
Figure 2C-4 Reagent Flow Diagram . . . . . . . . . . . . . . . . . . 5
Figure 2C-5 Sample Aspiration Flow Diagram. . . . . . . . . . . 6
Figure 2C-6 Dilution and Mixing Flow Diagram . . . . . . . . . . 7
Figure 2C-7 Sample Staging Flow Diagram . . . . . . . . . . . . 8
Figure 2C-8 Sample Delivery Flow Diagram . . . . . . . . . . . . 9
Figure 2C-9 Waste Flow Diagram . . . . . . . . . . . . . . . . . . . 10
Figure 2C-10 Vent Flow Diagram . . . . . . . . . . . . . . . . . . . . 11
Service Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2C - 1
MUFFLER
BLOOD/IN
EMPTY
SENSORS
PRES
PUMP
62
63
61
64
HGB DIL/IN
FILTER
WBC LYSE
FRONT VALVE
DILUENT
RESERVOIR
REAR VALVE
HGB LYSE
SHEATH
RESERVOIR
28
23
PS1
TOP
SENSOR
RBC/OUT
TO AIR
TO AIR
HGB/OUT
DILUENT
WBC LYSE
WASTE
TO AIR
BLOOD/OUT, FARSIDE
WBC/OUT
HGB LYSE
ACC 1
PRES 1
FILTER (SMALL)
TO AIR
(12 psi)
DILUENT (RED)
FILTER
(LARGE)
WBC
FLOW
CELL
91
65
96
94
DILUENT (RED)
1 2 3 4 5
56
53
WBC OUT
PS2
ACC 2
PRES 2
(9 psi)
95
RBC
MIXING
CHAMBER
52
HGB
12 uL
RBC
1.67 uL
WBC
M. C.
51
HGB OUT
WBC
20 uL
MOTOR-X
BLOOD
SAMPLE
VALVE
VENT
ACC
22
MOTOR-G
RBC DIL
RBC OUT
11
12
41
MOTOR-A
VENT
Y-VALVE
47,48
TO SAMPLE LOADER
(SL MODEL ONLY)
21
VAC
14
15
PUMP
92
TO AIR
26
25
38
MOTOR-E
24
33
10 mL
RBC
DIL
SYRINGE
SY4
34
WC-4
TO AIR
VENT
TRAP
WC-3
SENSOR
37
ACC 4
DRAIN
31
32
5
PS5
97
CLOSED
MODE
PROBE
98
MOTOR-C
WC-2
2.5 mL
HGB
LYSE
SY2
2.5 mL
WBC
LYSE
SYRINGE
SY3
MOTOR-D
VAC 1
(12" Hg)
68
17
27
OPEN
SAMPLE
PROBE
67
93
PRESSURE
SWITCH
VAC 1 (GRN)
PS4
500 uL
SAMPLE
INJECTION
SY1
TRAP
TRAP
MUFFLER
TRAP
HGB
FLOW
CELL
ULTRASONIC
SENSOR
13
SENSOR
TO AIR
PERISTALTIC
PUMP
55
HGB DIL
PS3
ACC 3
PRES 3
(5 psi)
57
WBC LYSE
SAMPLE
SENSOR 2
SENSOR
54
MOTOR-B
WC-1
18
36
VAC 1
(GRN)
VAC 2 (BRN)
SENSOR
PRESS 1 (ORN)
ACC 5
VAC 2
(VAR.)
VAC 2 (BRN)
AIR FLOW RESTRICTOR
32060
2C - 2
12" Hg.
5um
Filter
3-Way
Valve
NC
Out
.010" Orifice
Rack Advance
NO
V1
3-Way
Valve
Load Side Cross Transfer
NC
Out
.016" Orifice
NO
V2
3-Way
Valve
NC
Out
.016" Orifice
NO
V5
3-Way
Valve
To
Air
NC
Out
.016" Orifice
NO
V3
3-Way
Valve
5 psi
NC
Out
Mixer Bladders
NO
V4
Regulator
32061
2C - 3
WASTE
62
PRES
PUMP
63
61
64
SENSORS
TO AIR
TO AIR
FILTER
DILUENT
RESERVOIR
SHEATH
RESERVOIR
PS1
SENSOR
ACC 1
PRES 1
(12 psi)
12 psi
9 psi
PS2
5 psi
12" Hg
VAC 2 (Varies)
SENSOR
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW
ACC 2
PRES 2
(9 psi)
3
TRAP
PS3
SENSOR
ACC 3
PRES 3
(5 psi)
TRAP
TRAP
67
17
TO SAMPLE LOADER
(SL MODEL ONLY)
TO AIR
MUFFLER
VAC
PUMP
VAC 1 (GRN)
PS4
WC-2
WC-4
TO AIR
WC-1
WC-3
SENSOR
ACC 4
18
4
VAC 1
(12" Hg)
DRAIN
5
PS5
31
32
97
98
VAC 1
(GRN)
VAC 2 (BRN)
SENSOR
PRESS 1 (ORN)
ACC 5
VAC 2
(VAR.)
32062
DRAIN
VAC 2 (BRN)
2C - 4
BLOOD/OUT, FARSIDE
WBC/OUT
HGB LYSE
DILUENT
WBC LYSE
HGB/OUT
BLOOD/IN
EMPTY
SENSORS
HGB DIL/IN
RBC/OUT
WBC LYSE
FRONT VALVE
DILUENT
RESERVOIR
REAR VALVE
HGB LYSE
SHEATH
RESERVOIR
28
23
TOP
FILTER (SMALL)
WBC
FLOW
CELL
91
65
DILUENT (RED)
96
94
DILUENT (RED)
12345
WBC LYSE
95
RBC
MIXING
CHAMBER
MOTOR-X
BLOOD
SAMPLE
VALVE
WBC
20 uL
HGB
12 uL
RBC
1.67 uL
WBC
M. C.
HGB OUT
HGB DIL
22
MOTOR-G
RBC DIL
RBC OUT
HGB
FLOW
CELL
11
500 uL
SAMPLE
INJECTION
SY1
DILUENT/SHEATH
MOTOR-A
SHEATH
HGB LYSE
PRESSURE
SWITCH
WBC LYSE
15
27
OPEN
SAMPLE
PROBE
25
10 mL
RBC
DIL
SYRINGE
SY4
34
24
33
MOTOR-E
2 WAY PINCH VALVE NORMALLY CLOSED
26
2.5 mL
HGB
LYSE
SY2
2.5 mL
WBC
LYSE
SYRINGE
SY3
MOTOR-D
MOTOR-C
32063
MOTOR-B
CLOSED
MODE
PROBE
2C - 5
SAMPLE
SENSOR 2
WBC
20 uL
MOTOR-X
BLOOD
SAMPLE
VALVE
HGB
12 uL
RBC
1.67 uL
ULTRASONIC
SENSOR
12
Y-VALVE
47,48
TO AIR
TRAP
17
OPEN
SAMPLE
PROBE
38
WC-2
MOTOR-E
VENT
TRAP
CLOSED
MODE
32064
PS5
VAC 2 (BRN)
SENSOR
ACC 5
VAC 2
(VAR.)
VACUUM #2
2C - 6
(1) RBC/PLT
WBC
FLOW
CELL
TO AIR
FILTER
(LARGE)
1 2345
54
53
52
(2) WBC
WBC
M. C.
51
(3) NOC
55
VENT
ACC
PERISTALTIC
PUMP
MOTOR-G
RBC
MIXING
CHAMBER
HGB
FLOW
CELL
41
32066
RBC/PLT STAGING
WBC STAGING
NOC STAGING
STAGING PUMP LINES
WC-1
2C - 7
(1) RBC/PLT
WBC
FLOW
CELL
TO AIR
FILTER
(LARGE)
1 2345
54
53
52
(2) WBC
WBC
M. C.
51
(3) NOC
55
VENT
ACC
PERISTALTIC
PUMP
MOTOR-G
RBC
MIXING
CHAMBER
HGB
FLOW
CELL
41
32066
RBC/PLT STAGING
WBC STAGING
NOC STAGING
STAGING PUMP LINES
WC-1
2C - 8
SHEATH
RESERVOIR
TOP
TO AIR
FILTER (SMALL)
WBC
FLOW
CELL
91
65
DILUENT (RED)
FILTER
(LARGE)
12345
56
(1) RBC
(2) WBC
(3) NOC
VENT
ACC
500 uL
SAMPLE
INJECTION
SY1
MOTOR-A
68
2 WAY PINCH VALVE NORMALLY OPEN
SAMPLE DELIVERY
FLOW CELL OUT
SHEATH FLOW
32067
WC-1
2C - 9
WBC/OUT
HGB/OUT
BLOOD/IN
WASTE
HGB DIL/IN
RBC/OUT
FRONT VALVE
REAR VALVE
WBC
FLOW
CELL
12345
56
57
52
WBC
M. C.
WBC
20 uL HGB
12 uL
RBC
1.67 uL
MOTOR-G
RBC
MIXING
CHAMBER
TRAP
HGB
FLOW
CELL
12
92
13
32068
PERISTALTIC
PUMP
TRAP
TRAP
93
Y-VALVE
47,48
21
OPEN
SAMPLE
PROBE
14
WC-2
MOTOR-E
WC-4
WC-1
VENT
TRAP
WC-3
37
ACC 4
VAC 1
(12" Hg)
CLOSED
MODE
PROBE
DRAIN
36
ACC 5
VAC 2
(VAR.)
WASTE (BLK)
2C - 10
MUFFLER
2 WAY PINCH VALVE NORMALLY CLOSED
PRES
PUMP
TO AIR
TO AIR
FILTER
TO AIR
VENT TO AIR
FILTER
(LARGE)
ACC 1
PRES 1
53
(12 psi)
51
WBC
M. C.
VENT
ACC
RBC
MIXING
CHAMBER
HGB
FLOW
CELL
TO AIR
MUFFLER
68
VAC
TO AIR
PUMP
TO AIR
38
32069
WC-1
ACC 4
VAC 1
(12" Hg)
4
VENT
TRAP
2C - 11
DIAGNOSTICS
LATEX/BLOOD COMPARE
TOGGLE CS/SL
DIAG FUNCTIONS
ERROR LOG
From C:\CD3200
prompt:
PEEK COMMAND
POKE COMMAND
MOTOR CONTROL
CALIBRATION
2C - 12
6#0#3DUWV#/LVWV
Table of Contents
How to Use This Chapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Reagents and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Alphabetical Parts List Cross Reference . . . . . . . . . . . . . . . . . 4
PL-1 Power Supply Module . . . . . . . . . . . . . . . . . . . . . . 11
PL-2 Power Distribution Module & Laser Supply. . . . . . 13
PL-3 Reagent Lines & Fans . . . . . . . . . . . . . . . . . . . . . 16
PL-4 Flow Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
PL-5 Vacuum/Pressure . . . . . . . . . . . . . . . . . . . . . . . . . 22
PL-6 Aspiration Tower & Open Probe Assemblies). . . . 24
PL-7 Syringe Drivers (AB, CD) . . . . . . . . . . . . . . . . . . . 27
PL-8 Peri-Pump, Sample Handler Module #1,
& Open Probe Assembly. . . . . . . . . . . . . . . . . . . . 29
PL-9 Shear Valve Driver, Motor Driver Module,
& Sample Sensor Boards . . . . . . . . . . . . . . . . . . . 31
PL-10 Laser Bench . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
PL-11 Motor Processor Module. . . . . . . . . . . . . . . . . . . . 36
PL-12 Main Amplifier & Signal Processor Modules . . . . . 38
PL-13 Central Processing Unit/Device Control Module. . 40
PL-14 Flow Control Module, View #1 . . . . . . . . . . . . . . . 42
PL-15 Solenoid Driver Modules 1, 2, & Solenoid
Driver Module/Cable Distribution Module . . . . . . . 45
PL-16 Solenoid Driver Modules 5,6,9 & Cable
Distribution Module #2 . . . . . . . . . . . . . . . . . . . . . 48
PL-17 Main Computer Boards & Computer Assemblies . 51
PL-18 Sample Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
PL-19 Covers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Flow Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Pneumatic Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
CELL-DYN 3200 SYSTEM SERVICE MANUAL
Sample Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Tubing Designator/Catalog Number Table . . . . . . . . . . . . 66
3-1
Find the part in the drawing, note its item number, and
click anywhere on the drawing to jump to the
appropriate Parts Description table.
From a Tubing Map:
3-2
Part Number
Description
99120-01
93111-01
99129-01
03H78-01
03H79-01
03H80-01
99644-01
Enzymatic Cleaner
1360-02
8160580701
Versalube
10
8160600301
11
8160600401
12
8160600501
13
06H63-01
14
04H87-01
15
92532-01
16
06H71-01
Specifications, Interface
17
8938080601
18
8938070001
Disk, Service
19
06H61-01
Disk, Setup
20
06H90-01
21
06H91-01
22
8945020201
3-3
List #
Description
List #
PL-18
PL-17
PL-9
PL-12
Rea/Sup
Rea/Sup
Rea/Sup
PL-15 PL-16
PL-5
PL-19
PL-5
PL-9
Barcode Reader
PL-18
PL-17
PL-16
PL-5
PL-6
PL-8
Bd, CPU/DCM
PL-13
Belt, Timing
PL-7
PL-9
PL-6
PL-14
PL-6
Bd, HV/Preamp
PL-10
PL-6
PL-17
PL-10
PL-3
PL-6
PL-12
PL-6
PL-17
PL-4
PL-11
PL-4
PL-2
PL-4
PL-10
PL-17
PL-5
PL-18
PL-10 PL-12
PL-9
PL-10 PL-12
Bd, SDM/CDM
PL-15
PL-16
PL-7
3-4
List #
Description
List #
PL-16
PL-14 PL-3
PL-16
PL-14
PL-3
PL-14
PL-16
PL-14
PL-13
PL-14
PL-12
PL-14
PL-13
PL-17
PL-13
PL-17
PL-13
PL-4
PL-11 PL-13
PL-13
PL-13
PL-17
PL-13
PL-17
PL-16
PL-12
PL-12
PL-12
PL-14
PL-12
PL-14
PL-12
PL-5
PL-12
PL-14
PL-9
PL-4
PL-9
PL-14
PL-7 PL-11
PL-14
PL-7 PL-11
PL-14
PL-7
PL-14
PL-7 PL-11
PL-14
PL-11
PL-14
PL-6
PL-16
PL-13
PL-13
PL-19
PL-13
PL-11
3-5
List #
PL-8 PL-11
Description
List #
PL-12 PL-13
PL-14
PL-14
PL-16
PL-16
PL-8 PL-19
PL-16
PL-14
PL-16
PL-2 PL-17
PL-2 PL-17
PL-5 PL-13
PL-14
PL-2
PL-8
PL-2
PL-8
PL-2 PL-17
PL-8
PL-2 PL-13
PL-8
PL-2 PL-9
PL-8
PL-2 PL-11
PL-8
PL-2 PL-5
PL-18
PL-10
PL-18
PL-17
PL-18
PL-1
PL-18
PL-1
PL-18
PL-1 PL-2
PL-18
PL-1 PL-15
PL-18
PL-1
PL-12
PL-1
PL-9 PL-14
PL-1 PL-2
PL-9
PL-1
PL-9
PL-1
PL-9
PL-15
3-6
List #
Description
List #
PL-9
PL-19
PL-5
Cover, SL Bottom
PL-19
PL-5
PL-19
PL-5
PL-19
PL-5
PL-19
PL-5
Cover, Top
PL-19
PL-5
PL-18
PL-3
PL-18
PL-8 PL-9
PL-17
Rea/Sup
PL-17
PL-3
Disk, Service
Rea/Sup
PL-3
Disk, Setup
Rea/Sup
PL-3
Rea/Sup
PL-15
PL-8
PL-15
Enzymatic Cleaner
Rea/Sup
PL-4
PL-3
TM
PL-3
PL-17
TM
Rea/Sup
PL-4
PL-1
PL-5
PL-19
PL-4
Cover, Keyboard
PL-17
PL-7
PL-19
PL-3
PL-19
PL-3
PL-19
Rea/Sup
3-7
Description
List #
PL-4
PL-10
Fitting (T4)
TM
List #
Keyboard, PCAT
PL-17
PL-4
PL-19
TM
PL-3
TM
Rea/Sup
TM
Rea/Sup
TM
PL-9
TM
PL-10
TM
Mirror Perforated
PL-10
TM
PL-18
TM
PL-17
TM
PL-6
TM
Motor, Stepper
TM
PL-6
PL- 8
TM
PL-6
TM
PL-18
TM
Motor, Y-Valve
PL-8
TM
PL-6
TM
Plug
TM
PL-1
PL-2
PL-5
PL-1
PL-1
PL-6
PL-2
PL-6
PL-1
PL-8
Rea/Sup
PL-5
PL-5
PL-7
3-8
List #
Description
List #
PL-8
PL-18
PL-5
PL-19
PL-5
PL-7
Rea/Sup
PL-7
Rea/Sup
PL-7
Rea/Sup
PL-7
Rea/Sup
PL-7
Rea/Sup
TM
Regulator, Pressure
PL-18
TM
Reservoir, Diluent/Sheath
PL-4
TM
PL-18
TM
Sensor, Overpressure
PL-4
TM
PL-6 PL-8
PL-18 PL-19
TM
TM
PL-9
TM
PL-8
TM
PL-9
TM
PL-18
TM
PL-9
TM
PL-9
TM
PL-9
TM
PL-6
TM
Speaker Assy
PL-17
TM
Specifications, Interface
Rea/Sup
TM
PL-17
TM
3-9
List #
TM
TM
PL-6
PL-4
PL-3
PL-10
PL-10
PL-8
PL-4
PL-5
PL-5
PL-4
TM
PL-8
PL-18
Versalube
Rea/Sup
3 - 10
9
10
11
6
13/14
5
4
3
2
12
3 - 11
Part Number
Description
1
2
3
4
5
6
7
8
9
10
11
12
13
8934000801
8951078201
8952086901
8952082801
8952086601
8510016601
8952087801
8952062001
8952000101
8952000201
8952089601
8240051601
50065-01
14
61164-01
3 - 12
7
8
9
10
11
3
2
1
12
3 - 13
13
14
3 - 14
Part Number
8952078201
8952082901
8952054301
8952089601
8952089701
8952089801
8952061201
8952086501
8952078901
8952089401
8952089501
8960198001
8510019201
8921207601
Description
Cable, PSM (B)-J4 to PDM-J9
Cable, PDM-J8 to FCM-J21
Cable, PDM-J6 to MPM-J2
Cable, PSM (T)-J501 to PDM-J1
Cable, PDM-J10 to Hard Drive
Cable, PDM-J11 to Floppy Drive
Cable, PDM-J3 to Mother Bd.
Cable, PDM-J4 to MAM-J3
Cable, PDM-J5 to MDM-J3
Cable, PDM-J12 to SHM1-J12
Cable, PDM-J13 to SHM2-J12
Bd, PDM (Power Dist. Module)
Fuse, Fast 1A 250V 3 AG
Power Supply Laser
3 - 15
4
3
6
3 - 16
11
13
32017
12
3 - 17
Part Number
06H65-01
8921210101
8921205901
8921211501
92161-02
8925089901
8960091001
8952087001
03484-01
8260402701
8952085701
8930038301
8930067601
Description
Kit, Reagent Line (Complete)
Cap Assy, WBC, Lyse
Cap Assy, Diluent/Sheath
Cap Assy, HGB/NOC Lyse
Tube Assy, Waste Outlet
Cable, Waste Full Sensor
Bd, In-line Sensor
Cable, FCM-J26 to ILSB-J1
Fan Guards & Filter, 3 Pcs
Fan, 115VAC, 4.69 SQ
Cable, CDM2-J7 to Ext Waste
Fitting, Reagent Inlet
Fitting, Waste Outlet
3 - 18
3 - 19
8
14
6
5
4
13
15
11
9
10
12
32019
3 - 20
Part Number
8921122501
8921016301
8913007601
8921174702
8270721901
8921176502
8921174902
8921206702
8921150901
8510615001
8921174802
8270721901
8921210801
8952077801
8921217201
Description
Valve Assy, Solenoid, 3.9KG
Valve Assy, Solenoid, NC
Kit, Hardware, 3200
Bottle, Waste (small)
Filter, Vent Accumulator
Bottle, Vent Accumulator
Reservoir, Diluent/Sheath
Flow Cell Assy, HGB
Trap Assy, Waste Bottle
Sensor, Overpressure
Bottle, Waste (large)
Filter, In-line, Sheath
Chamber, Mixing, RBC/WBC
Cable, HGB Flow Cell to FCM
Bottle, Waste (small w/tubing)
3 - 21
PL-5 Vacuum/Pressure (1 of 2)
10
11
13
8
12
22
V1
3
3
V4
V2
V3
V5
4
6
15
16
1
5
14
17
2
19
1
18
21
20
3 - 22
PL 5 Vacuum/Pressure (2 of 2)
Item
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
Part #
8921122001
8913007601
8921173201
8921122101
8921153401
8921153001
8410301801
60160-01
8960120001
8952083401
8921173401
8952087501
8952052801
8952082901
8952083101
8952083201
8952084101
8952084901
8952087901
8952083401
8960122001
8270040301
Description
Valve Assy, Solenoid, 5Kg
Kit, Hardware, 3200
Accumulator, Vac/Pres (SM)
Valve Assy, Solenoid, 3.9KG
Pump, Vacuum (Complete)
Pump, Pressure (Complete)
Pump Head, Vac
Fuse, 5A 220/240 Vac
Bd, PRM (Pump Relay Module)
Cable, VPM-J18 to PRM-J1
Accumulator, Vac/Pres (Lg)
Cable, Vac2 Wet Sensor
Cable, Vac1 Wet Sensor
Cable, PDM-J8 to FCM-J21/VPM-J12
Cable, VPM-J13 to FCM-J23
Cable, VPM-J14 to FCM-J24
Cable, MUX DAC CTRL Bus
Cable, Serial Periph Bus
Cable, VPM-J6 to CDM2-J4
Cable, VPM-J18 to PRM-J1
Bd, VPM (Vac/Pres Module)
Filter, Pressure Pump
3 - 23
7
2
10
5
8
3 - 24
11
12
15
13
14
3 - 25
Part #
Description
8921208502
8370830101
8250426601
8250426501
8921209202
8250426801
03H99-01
8933040501
8934005801
10
8350602701
11
8921204601
12
9960042001
13
8952084201
14
8921076101
Motor, Stepper
15
8931201401
16
8930094601
3 - 26
8
5
5
4
4
7
10
9
6
12
13
9
1
2
6
5
11
6
5
3 - 27
Part #
Description
8921217301
28561-01
28560-01
04H02-01
8250425701
Belt, Timing
8921076101
Motor, Stepper
8952084401
8952015401
8960042001
10
8952015501
11
8921217401
12
02H82-01
13
8952084501
3 - 28
6#0#3DUWV#/LVWV #PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (1 of 2)
PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (1 of 2)
11
12
13
10
9
14
8
7
6
15
1
2
16
16
3
5
CELL-DYN 3200 SYSTEM SERVICE MANUAL
17
19
18
17
3 - 29
6#0#3DUWV#/LVWV #PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (2 of 2)
PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (2 of 2)
Item
Part #
Description
8921137801
8952056301
8921076101
Motor, Stepper
91485-01
8921071001
8960191001
8952089201
8952088301
8952089401
10
8952088401
11
8952088201
12
8952078001
13
8952087201
14
8921206101
15
8921207701
Motor, Y-Valve
16
8370830101
17
8952087101
18
8250900301
19
8370181801
3 - 30
6#0#3DUWV#/LVWV #PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (1 of 3)
PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (1 of 3)
8
9
10
11
12
1
4
7
5
6
3 - 31
6#0#3DUWV#/LVWV #PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (2 of 3)
PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (2 of 3)
18
19
P/O Bd.
20
13
17
16
15
14
3 - 32
6#0#3DUWV#/LVWV #PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (3 of 3)
PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (3 of 3)
Item
Part #
Description
8921204901
8960116701
N/A
8932102801
06H64-01
8932103101
8921167901
8960222001
8952086201
10
8952078501
11
8952086901
12
8952087401
13
8960194001
14
8952078901
15
8952079601
16
8952079501
17
8952087101
18
8370181801
19
8960116701
20
8952082001
3 - 33
4
5
1
10
6
5
8
7
11
3 - 34
Part #
Description
8921206401
8010131201
8921137501
8570000101
8960100001
8960101001
Bd, HV/Preamp
8921142901
8952085501
8952085601
10
8921145201
11
8921181501
Mirror Perforated
3 - 35
7
8
9
3 - 36
Part #
Description
8960092001
8952084701
8952054301
8952084501
8952084401
8952015501
8952015401
8952084201
8952056301
3 - 37
2
3
13
8
12
11
10
6
9
8
7
6
CELL-DYN 3200 SYSTEM SERVICE MANUAL
3 - 38
Part #
Description
8960195001
8952085501
8952085601
8960183001
8952086501
8952084801
8952050001
8952084101
8952050501
10
8952084601
11
8952085401
12
8952085301
13
8952050601
3 - 39
6
4
3
5
11
12
13
15
10
14
3 - 40
Part #
Description
8960182001
Bd, CPU/DCM
8952084901
8952086401
8952084701
8952084601
8952089301
8952089201
8952084101
8952050001
10
8952086501
11
8952085001
12
8952050601
13
8952085201
14
8952050501
15
8952085101
3 - 41
10
8
7
3 - 42
21
20
19
18
16
17
15
14
13
12
11
3 - 43
Part #
Description
8960132001
8952084101
8952084901
8952082501
8952082601
8952087001
8952083701
8952086101
8952086601
10
8952083801
11
8952082901
12
8952085101
13
8952083101
14
8952083201
15
8952085201
16
8952038501
17
8952082001
18
N/A
19
8952083901
20
8952078301
21
8952086201
3 - 44
1
6
V41
V38
V32
V36
5
V34
V33
4
V32 V31
3
6
6
3
5
6
3 - 45
V18
V21
V17
V22
V23
V15
V24
V25
V14
V13
V11 V12
V26
V27 V28
3 - 46
Part #
Description
8960205001
Bd, SDM/CDM
8952082801
8952083001
8952085801
8960093001
8952083701
3 - 47
6#0#3DUWV#/LVWV #PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (1 of 3)
12
1
2
1
11
8
10
9
2
8
6
5
3
4
3 - 48
6#0#3DUWV#/LVWV #PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (2 of 3)
V94
V93
V92 V91
V68
V67
V57
V56
V66
V55
V65
V54
V64
V63
V62
V61
V53
V52
V51
3 - 49
6#0#3DUWV#/LVWV #PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (3 of 3)
Part #
Description
8960093001
8952085901
8960108001
8952085701
8952083501
8952083601
8952082601
8952086001
8952086601
10
8952087901
11
8952083701
12
8952086101
3 - 50
4
3
5
2
7
3 - 51
15
15
14
11
10
16
8
3
9
13
12
3 - 52
20
18
17
20
22
16
21
21
3 - 53
Part #
Description
8200570201
8921150601
Speaker Assy
8952056201
8952089801
8200570701
8952064801
8952089701
8921205401
8921211901
10
8952061201
11
8170001301
12
8960109001
13
8952082401
14
8170000701
15
8600028501
16
06H74-01
Keyboard, PCAT
17
06H75-01
Cover, Keyboard
18
04H44-01
19
8510751801
20
8952087017
21
20005-01
3 - 54
7
13
#
11
12
16
15
14
2
3 - 55
19
18
7
11
17
19
32071
3 - 56
7
19
7
11
32072
11
3 - 57
1
4
6
V5
V4
32073
V3
V2
V1
3 - 58
Part #
Description
8960191001
8952058801
8952058601
8952089301
8952089001
8952089501
8952088701
Part of Sensor
8921211601
10
8350602501
11
8370830101
12
8934001701
Barcode Reader
13
8921208301
14
8960211001
15
2880860801
16
8510510101
17
8450876601
18
8310842201
Regulator, Pressure
19
8280861301
20
8932074001
3 - 59
PL-19 Covers (1 of 3)
6
8
9
6
2
17
8
13
12
8
1
32074
11
10
3 - 60
PL-19 Covers (2 of 3)
11
20
19
18
32075
3 - 61
PL-19 Covers (3 of 3)
Item
Part #
Description
8931213001
Cover, SL Bottom
8932063701
8932073701
8932073801
8921217001
8913007601
8931204801
8913007601
8931204601
Cover, Top
10
8931204701
11
8932062201
12
8370830101
13
8952078001
17
8932062101
18
8510810901
19
8952083801
20
8960192001
3 - 62
Flow Panel
3 - 63
Pneumatic Unit
3 - 64
Sample Loader
3 - 65
Part #
Description
A1
8310817501
A2
8310817401
8310808401
C1
8310802001
CLP2
8210050201
CV
8310810201
EL
8310820001
F1
8270722201
L1
8310807901
L7
8310805801
P1
03H85-01
Plug
R1
8310805901
R2
8310800101
R3
8310806201
R4
8310807401
S1
9130542
S2
9130543
SY
9130544
T1
8310801001
T3
8310800901
T4
31082-01
Fitting (T4)
T5
8310809501
T8
8310800701
3 - 66
Part #
Description
Y1
9130548
Y2
9130549
Y3
8310654201
Y4
9130555
Y5
8310654001
Y622
8310662201
Y626
8310662601
Y627
8310662701
Y630
8310663001
Y631
8310663101
Y632
8310663201
Y633
8310663301
Y634
8310663401
Y635
8310663501
Y636
8310663601
Y637
8310663701
Y638
8310663801
Y7
8310653501
Y FIT
8310810801
3 - 67
7#0#5HPRYDO#)#5HSODFHPHQW
Table of Contents
Introduction
4-1
Figure 4-1:
Removal Procedure
1. Ensure that the shear valve is in the
cleaning position using the special
protocols function.
2. With analyzer power off, open the left
and right access doors and remove
the safety cover.
4-2
Figure 4-2:
4-3
Figure 4-3:
4-4
Figure 4-4:
4-5
Figure 4-5:
4-6
Figure 4-6:
4-7
Figure 4-7:
Replacement Procedure
Replace by following the steps above in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
4-8
Y-Valve (RR-2)
Figure 4-8:
Removal Procedure
1. With analyzer power off, open the left
and right access doors and remove
the front cover.
4-9
Figure 4-9:
4 - 10
4 - 11
4 - 12
4 - 13
4 - 14
Replacement Procedure
Follow the above steps in reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
4 - 15
Removal Procedure
4 - 16
4 - 17
4 - 18
4 - 19
4 - 20
Replacement Procedure
Follow the above steps in reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
4 - 21
(RR-4)
Removal Procedure
Warning Potential Biohazard:
The aspiration needle/probe is sharp
and potentially contaminated with
infectious materials. Avoid contact
with the tips of the needle/probe.
4 - 22
4 - 23
4 - 24
4 - 25
4 - 26
4 - 27
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
4 - 28
Removal Procedure
'$1*(5=
High Voltage: Assure the
power to the instrument is
off. A high voltage charge
may remain on the laser
connector with the power
off. Do not touch the male
pins. Short male pins to
Chassis.
1. With the analyzer power off, remove
the top cover (4 screws) and the optics
cover (4 screws).
4 - 29
4 - 30
4 - 31
4 - 32
4 - 33
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
4 - 34
Removal Procedure
1. With analyzer power off, remove
optics bench (RR-5).
2. Disconnect the nine connectors
shown on the power supply.
4 - 35
4 - 36
4 - 37
Figure 4-37: Power Supply Line Frequency & Line Voltage Select Switches
Verification Procedure
See Verification Matrix, Section 5.2.
4 - 38
Vacuum/Pressure Assembly
(RR-7)
Removal Procedure
NOTE: It is no longer necessary to
remove the Vacuum/Pressure
Assembly when servicing the VPM,
pumps, etc. The Assembly is mounted
on movable slides in 3200 analyzers
built after May, 1998. To access the
Assembly:
1. With the analyzer power off, remove
the left side panel (4 screws).
4 - 39
4 - 40
4 - 41
Verification Procedure
See Verification Matrix, Section 5.2.
4 - 42
Removal Procedure
Warning Potential Biohazard:
The aspiration needle/probe is sharp
and potentially contaminated with
infectious materials. Avoid contact
with the tips of the needle/probe.
4 - 43
4 - 44
4 - 45
4 - 46
4 - 47
4 - 48
4 - 49
4 - 50
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
4 - 51
Removal Procedure
1. With analyzer power off, open left and
right access doors and remove front
cover.
4 - 52
4 - 53
4 - 54
4 - 55
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
4 - 56
Removal Procedure
Cell-Dyn 3200 Analyzers built after May
1998 do not require sample loader removal
to access front panel.
Follow Part A to access front panel. Follow
Parts A and B to remove and replace the
loader.
Part A
1. With analyzer power off, open the left
and right access doors and remove
the safety cover.
4 - 57
4 - 58
4 - 59
4 - 60
4 - 61
4 - 62
4 - 63
4 - 64
4 - 65
4 - 66
4 - 67
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
4 - 68
Removal Procedure
1. With analyzer power off, open the left
and right access doors and remove
the safety cover.
4 - 69
4 - 70
4 - 71
4 - 72
4 - 73
4 - 74
4 - 75
Replacement Procedure
Replace by following the above steps in
reverse order.
Verification Procedure
See Verification Matrix, Section 5.2.
4 - 76
8#0#9HULILFDWLRQ#3URFHGXUHV
Table of Contents
5.1
5.2
5.3
VP-22
VP-23
VP-24
VP-25
VP-26
VP-27
VP-28
VP-29
VP-30
VP-31
VP-32
VP-33
VP-34
VP-35
VP-36
VP-37
VP-38
VP-39
VP-40
VP-41
VP-42
VP-43
VP-44
VP-45
VP-46
VP-47
VP-48
VP-49
5-1
Menu Selections
1. From Main Menu, press
DIAGNOSTICS
MORE
TOWER TEST
or:
a. Press
ACCEPT
User Entries
User-typed entries are shown in bold, italicized type. For
example:
a. At C:\ prompt type CD\RAWFILE, and press
ENTER
ENTER
5-2
REPLACED ASSEMBLY
ASSOCIATED VPs
(Perform In Order Listed)
VP-35, VP-36, VP-37, VP-38,
VP-39, VP-40, VP-41
REPLACED ASSEMBLY
ASSOCIATED VPs
(Perform In Order Listed)
0 Degree Preamp
10 Degree Preamp
VP-7
Sample Sensor #2
VP-12
VP-17, VP-16
Barcode Reader
VP-10, VP-40
Aspiration Tower
Sample Loader
Optics Bench
CPU/DCM
Laser Head
VP-5, VP-9
VP-4, VP-16, VP-56, VP-57,
VP-58
VP-6, VP-7
VP-46
VP-9
Peri-Pump Assembly
5-3
ASSOCIATED VPs
(Perform In Order Listed)
VP-53, VP-54
VP-55
5-4
Table 5-1
Voltage Specifications
Test Point
Function
Range
Ripple
PSM-TP4
Analog Ground
N/A
N/A
PSM-TP2
15.0V-19.6V
< 3.0V PP
# 2 Phillips Screwdriver
PSM-TP1
28.9V-37.7V
< 7.0V PP
Digital Voltmeter
PSM-TP7
N/A
N/A
N/A
Clip Leads
PSM-TP6
N/A
N/A
N/A
PSM-TP3
+15 VDC
14.6V-15.4V
< 75MV PP
PSM-TP5
-15 VDC
14.6V-15.4V
< 75MV PP
N/A
-12 VDC
11.50-12.50V
<75MV PP
N/A
+12 VDC
11.50-12.50V
<75MV PP
N/A
+5 VDC
5.00V-5.40V
<75MV PP
N/A
24.0-25.0
<75MV PP
Verification Procedure:
1. Open right side door to gain access to power supply.
2. Refer to Figure 5-1. Connect voltmeter ground lead to TP4
on power supply module.
3. Verify the voltages according to Table 5-1.
5-5
REAR
TO P
15 A S B (+ 1 2V )
F1
ALARM
T P 1 + 24 V
F2
-12V
T P 2 + 12 V
15 A S B (+ 2 4V )
PSM
+ 12 V
T P 3 + 15 V
TP4
GND
TP5
-15V
+ 24 V
GND
+ 5V
10 0V
TP6 RET
+ 10 0V
TP7
3 2 0 76
# 2 Phillips Screwdriver
Digital Voltmeter
Clip Leads
Verification Procedure:
1. Open right side door to gain access to the CPU/DCM.
Part Number 9140300 August 1998
5-6
Verification Procedure:
1. Connect positive lead of DVM to TP2 and negative to TP7.
2. If necessary, adjust R86 for a reading of 10.00 volts +/0.01V.
DIAGNOSTICS
# 2 Phillips Screwdriver
MORE
Digital Voltmeter
VOLTAGE READINGS
Clip Leads
Verification Procedure:
# 2 Phillips Screwdriver
Digital Voltmeter
Clip Leads
5-7
RETURN
None
Verification Procedure:
RETURN
8. Press
VOLTAGE READINGS
DIAGNOSTICS
MORE
VOLTAGE READINGS
2. Move the cursor to HGB output, and press
SELECT
FINISH SELECT
3. Run one background count to insure that the HGB mixing
chamber is full of liquid.
4. Verify that the HGB OUTPUT voltage is 5.00 volts
+0.2/-0.00 volts. If the voltage is within range, skip steps 5
through 8.
5. Press
MORE
to the first diagnostic screen, and press
F12
F1
None
Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
MOTOR OPERATION
2. Press
MOTOR PWR CHECKING
and wait for the display.
5-8
2. Press
EXERCISE MOTORS
type the letter of the suspect assembly, and press
ENTER
Phase 0
Phase 0
Phase 0
Phase 3
Motor
Low
Medium
High
High
1.5-2.1
3.4-4.2
4.9-5.9
4.9-5.9
.6-1.2
2.5-3.1
4.2-5.0
4.2-5.0
.6-1.2
2.5-3.1
4.2-5.0
4.2-5.0
.6-1.2
2.5-3.1
4.2-5.0
4.2-5.0
1.5-2.1
3.4-4.2
4.9-5.9
4.9-5.9
1.5-2.1
3.4-4.2
4.9-5.9
4.9-5.9
None
Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
None
Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
MOTOR OPERATION
CELL-DYN 3200 SYSTEM SERVICE MANUAL
MORE
MOTOR OPERATION
2. Press
HOME MOTORS
type the letter of the suspect assembly, and press
ENTER
3. Verify that the assembly moves smoothly without stopping
or jerking.
Part Number 9140300 August 1998
5-9
Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
None
SOLENOID OPERATION
2. Press
STEP SOLENOID
Verification Procedure:
ENTER
DIAGNOSTICS
MORE
SOLENOID OPERATION
2. Press
CYCLE BANK
type the number of the bank, and press
ENTER
Verification Procedure:
None
5 - 10
Verification Procedure:
1. Remove top and top right covers.
2. Run controls and insure that results are within
specifications.
3. Create a specimen dilution using LOW ABNORMAL
CONTROL material and CELL-DYN 3200 Diluent/Sheath
that gives an RBC value of 0.8MEG/ul, +/- .03.
4. Connect the positive lead of the DVM to TP1 and the
negative lead to TP3 on the specimen sensor board.
Verification Procedure:
1. Turn power off, install loopback connector at COM1 (top
serial connector) on PC module, and power-up and
initialize instrument.
5 - 11
None
Verification Procedure:
1. Ensure that the instrument is in the READY mode.
2. Verify that off-time of the vacuum pump is 2 minutes or
greater between automatic recovery cycles.
3. Verify that off-time of the pressure pump is 2 minutes or
greater between automatic recovery cycles.
None
Verification Procedure:
5 - 12
None
Verification Procedure:
1. Ensure that the instrument is in the Open Sampler mode.
7. Press
MORE
SET ANALYZER
VOLTAGE READINGS
RETURN
Vacuum/Pressure
Specification
Pressure #1
12.5-13.5 psi
Pressure #2
8.5-9.5 psi
Pressure #3
4.0-4.5 psi
Vacuum #1
12.5-13.5 Hg
Vacuum #2
3.1-3.5 Hg
RETURN
NOTE: If the level is reduced the vacuum or pressure
must be bled off to the new level before performing step 8.
A count cycle may be used for bleeding.
8. Go to VOLTAGE READINGS, and repeat steps 3 through
8 until the level(s) are within specifications.
5. Press
MORE
CELL-DYN 3200 SYSTEM SERVICE MANUAL
5 - 13
None
Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
to VACUUM TEST and PRESSURE TEST.
2. Press
VACUUM TEST
and verify that the elapsed time is 4.5 seconds or less.
3. Press
PRESSURE TEST
and verify that the elapsed time is 3.5 seconds or less.
# 2 Phillips Screwdriver
Verification Procedure:
1. Ensure instrument power is off. Remove top cover, both
optics covers, and open flow cell access door.
'$1*(5=#+,*+#92/7$*(#Assure the power
to the instrument is off. A high voltage charge may
remain on the laser connector with the power off.
Do not touch the male pins. Disconnect the laser
connector and short both male pins to the
instrument chassis.
2. Disconnect laser connector and short both male pins to
instrument chassis.
3. Remove Laser Clamps and replace Laser.
4. Replace clamps and tighten until snug but Laser can still
be moved.
5. Move laser body until 3/4 inch extends beyond front laser
clamp and rotate laser body until front label is on top.
6. Connect laser connector, ensure laser shutter is closed,
turn on power, and prime instrument. Allow a 15-minute
laser warm-up period.
7. Refer to Figure 5-4. Mount Power Meter in beam path.
Open Laser Shutter and verify a reading of no less
than 5 mW.
8. If you have a polarizing filter, skip step 9.
9. Scribe optics bench on right and front sides of beam
splitter/polarizer assembly, and remove assembly from
90o path.
5 - 14
56
WBC
FLO W
C EL L
91
3
FLO W C E LL M AN IFO LD
4
54
1
57
2
52
32 077
21. Observe the 90o slit. When two red images of the flow cell
walls appear on the slit, close solenoid 56 and reconnect
tubing to port #1.
22. Adjust the Y alignment screw on the front mirror for a
minimum reading on the DVM. This reading should be less
than 0.300 volts.
23. Adjust the Y alignment screw on the rear mirror until the
red images are in the center 1/3 of the 90o slit.
Part Number 9140300 August 1998
5 - 15
# 2 Phillips Screwdriver
Verification Procedure:
1. Remove top cover, both optics covers, and open flow cell
access door.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
5 - 16
56
WBC
FL O W
CELL
91
3
FL O W C E LL M A NIFO L D
4
57
54
1
2
52
32077
Figure 5-3
10. Observe the 90o slit. When two red images of the flow cell
walls appear on the slit, close solenoid 56 and reconnect
tubing to port #1.
11. Adjust the Y alignment screw on the front mirror for a
minimum reading on the DVM. This reading should be less
than 0.300 volts.
12. Adjust the Y alignment screw on the rear mirror until the
red images are in the center 1/3 of the 90o slit.
13. Repeat steps 11 and 12 until the red images are in the
center 1/3 of the slit and the DVM voltage is at minimum.
The minimum voltage should be less than 0.200 volts.
14. Re-check power transmitted through forward slit. If it has
changed, a slight X alignment of the rear mirror is
necessary.
The alignment of the optical flow cell is one of the most critical
alignments performed on the CELL-DYN 3200. It must be
optimized so that the instrument can properly perform the WBC
5-Part Differential measurements. However, if this alignment is
not really needed, it is best that it not be performed. Therefore,
if the instrument is within the prescribed specifications, do not
attempt to align the optical flow cell.
# 2 Phillips Screwdriver
Clean Container
Verification Procedure:
1. Prepare the WBC Beads solution.
a. Place 2mL of diluent in a clean container.
b. Add 15 drops of 7.00 beads and mix well.
2. From Main Menu, press
DIAGNOSTICS
MORE
WBC DATA
Part Number 9140300 August 1998
5 - 17
3. Press
CALC CV
4. Place Beads solution under probe and press TOUCH
PLATE to aspirate Beads and run a count cycle.
5. Refer to Table 5-4 and compare the CVs to those
displayed on the screen. If the displayed CVs are less than
those from the table, the flow cell does not need
alignment.
NOTE: If any or all of the CVs are out-of-specification, the
flow cell must be completely aligned. The 0o and 10o
channels must be aligned first by adjusting the Y-axis
alignment (lower) micrometer and then the 90o and 90o
depolarized must be aligned by adjusting the X-axis
alignment (right) micrometer.
Table 5-4
Channel
Mean
Range
CV
0o
35
32-38
<3.5%
10o
65
60-70
<3.5%
90o
5 - 18
5 - 19
X A xis
Laser S trap
Laser S trap
90 oD epolarized P M T
.75"
P lace B e am splitter/
P olarizer here during
Laser po larization.
90 oP M T
Y
X
P lace P o w er M eter
H ead he re during
Laser po larization.
A
x
i
s
M irror R e ar View
F ixture H oles
90 oO bjective Lens
10 o D etector
C ylinderc al Lens
Im aging Lens
0 oD etector
F low C ell
Y -A xis A lignm ent M ic.
F ront M irror
O bscura tion B ar
X -A xis A lignm ent M ic.
5 - 20
Clean Container
Beam Images
"B "
R ear W all
"A "
C lear Q u artz
S catter P attern
O bscuration B ar
"C "
F ront W all
"D "
F low C ell C entered
Verification Procedure:
1. Prepare the Bead solution.
320 79
B rightest Im age
5 - 21
# 2 Phillips Screwdriver
Clean Container
Verification Procedure:
1. Prepare the WBC Beads solution:
CALC CV
4. Place Beads solution under probe and press START
SWITCH to aspirate Beads and run a count cycle.
5. Observe the mean channel values for the 90o and 90o
depolarized channels, and refer to Table 5-4. If the
displayed mean channel values are within specifications
and the recorded gain settings are less than 2500, the
dynode voltages need not be adjusted and the procedure
is completed. If either of the above are out-of-range, the
dynode voltage(s) for the channel(s) must be adjusted.
6. Remove the top cover from the analyzer, and locate the
PMT preamplifiers on the right side of the optical bench.
The board on the left is for the 90o channel and the board
on the right is for the 90o depolarized channel. R11 adjusts
the dynode voltage on both boards.
7. Press
MORE
F12
F1
SET POINT ENTRY
5 - 22
14. Press
DIAGNOSTICS
MORE
9. Press
SET ANALYZER
RETURN
RETURN
MORE TO
WBC DATA
10. Cover optical bench with black cloth to block ambient light.
11. Place the 7.00 beads solution under the probe, and
press
EXTENDED WBC COUNT
START
to aspirate the Beads and start the cycle.
o
VOLTAGE READINGS
15. Verify that both dynode voltages are less than 9.00 (900
volts). Dynode voltages greater that 900 volts indicate a
problem, and the problem must be isolated and corrected
before continuing.
X Present Setting
5 - 23
Clean Container
Verification Procedure:
1. Prepare the WBC Beads solution:
a. Place 2mL of diluent in a clean container.
b. Add 15 drops of 7.00 beads and mix well.
2. From Main Menu, press
DIAGNOSTICS
MORE
GAIN ADJUSTMNTS
3. Press
VERIFY GAINS
WBC GAINS
4. If necessary, change the Mean Channel Numbers to the
desired values (Table 5-1) and press
ENTER
5. Place Beads solution under probe, press
START
and press the START SWITCH to aspirate Beads and
run a count cycle.
6. If the Mean Channel Numbers are within specifications,
skip steps 7 through 12.
7. Press
RETURN
to access AUTO GAIN ADJUSTMNT
8. Press
AUTO GAIN ADJUSTMNT
WBC GAINS
9. If necessary, change the Mean Channel Numbers to the
desired values (Table 5-1) and press ENTER.
NOTE: You must run Beads twice in order to set gains.
The first run is needed to calculate new settings, and the
second is to verify the settings.
10. Place Beads solution under probe, press
START
and press the START SWITCH to aspirate Beads and
run a count cycle.
11. Place Beads solution under probe and press START
SWITCH to aspirate Beads and run a count cycle to
verify.
12. Press
Y
ENTER
to save new gain settings.
5 - 24
9. Press
Clean Container
OPTI-CAL
11. Press
CELL REGION
RETURN
Verification/Adjustment Procedure:
13. Run the OPTI-CAL 3 times and record the mean channel
values for GRANs on 90o and 90oD on each run.
14. Compute the average mean channel for 90o and 90oD for
the three runs.
16. Calculate the new gain values for 90o and 90oD, use the
following equation:
X (Y/Z)
Where:
X = Current Gain Value.
DIAGNOSTICS
F12
F1
8. Press
BEADS/BLOOD COMPARE
17. Press
RETURN
SET POINT ENTRY
5 - 25
26. Press
MORE
ENTER
GAIN ADJUSTMNTS
19. Enter the new value for 90oD gain and press
ENTER
27. Press
SET ANALYZER
RETURN
WBC GAINS
20. Press
BEADS/BLOOD COMPARE
and repeat steps 12 through 15.
21. Toggle BLOOD/BEADS key to BEADS.
22. Prepare the Bead solution.
a. Place 2mL of diluent in a clean container.
b. Add 15 drops of 7.00 beads and mix well.
23. Run the Beads three times and record the Mean Channel
for 90o and 90oD on each run. Verify that 0o and 10o Mean
Channels are 35+/-3 and 65+/-5 respectively and all CVs
are within specification on each run.
24. Compute the average Mean Beads Channel for 90o and
90oD for the three runs.
NOTE: These Beads Channel locations are now specific
to this analyzer and must be entered into the AUTO GAIN
table.
28. Enter the Beads Channel numbers for 90o and 90oD.
29. Run an Auto Gain using Beads to enter these channel
locations into the default table.
30. Reboot system on a Setup Disk. Perform a GET to store
these values.
None
25. Press
RETURN
RETURN
5 - 26
Red-Top Vacutainer
Transfer Pipette
ENTER
5. Move the cursor to the NOC 10o setpoint.
6. Multiply the displayed WOC 10o setpoint by 1.5, change
the NOC 10o setpoint to the new calculated value, and
press
ENTER
7. If necessary, move the cursor to the NOC Lo threshold,
change it to 400, and press
ENTER
8. Press
SET ANALYZER
to store the new values.
9. Press
RETURN
Verification/Adjustment Procedure:
1. Prepare the 7.00 beads solution:
a. Mix the 7.00 beads by shaking vigorously.
b. Add 15 drops of 7.00 beads to a vacutainer.
c. Add 2 ml of 3200 Diluent/Sheath reagent.
2. From Main Menu, press
DIAGNOSTICS
MORE
GAIN ADJUSTMNTS
3. Press
VERIFY GAINS
RBC/PLT 0O GAIN
RETURN
CELL-DYN 3200 SYSTEM SERVICE MANUAL
5 - 27
START
ENTER
8. Press
AUTO GAIN ADJUSTMNT
RBC/PLT 0O GAIN
9. If necessary, change the Mean Channel Number to the
desired value and press
ENTER
NOTE: You must run Beads twice in order to set gain. The
first run is needed to calculate new setting, and the second
is to verify the setting.
10. Place Beads solution under probe, press
START
and press the START SWITCH to aspirate Beads and
run a count cycle.
Red-Top Vacutainer
Transfer Pipette
Verification/Adjustment Procedure:
1. Prepare the 3.29 or 3.31 Beads solution.
a. Mix the Beads by shaking vigorously.
b. Add 10 drops of Beads and 20 drops of diluent to a
vacutainer and mix well.
5 - 28
NOTE: You must run Beads twice in order to set gain. The
first run is needed to calculate new setting, and the second
is to verify the setting.
10. Place Beads solution under probe, press
START
and press the START SWITCH to aspirate Beads and
run a count cycle.
11. Place Beads solution under probe and press START
SWITCH to aspirate Beads and run a count cycle to
verify.
12. Press
Y
START
ENTER
ENTER
CELL-DYN 3200 SYSTEM SERVICE MANUAL
5 - 29
Transfer Pipette
Verification/Adjustment Procedure:
Beads
RBC 0o
P.C.
RBC 10o
P.C.
PLT 0o P.C.
3.29
N/A
N/A
N/A
Set at 120
4.99
Falls at
129
N/A
N/A
N/A
7.00
Set at
180
Falls at 200
N/A
N/A
0o Equation:
7.00 RBC 0o P.C.
4.99 RBC 0o P.C.
DIAGNOSTICS
MORE
RBC DATA
10 Equation:
7.00 RBC 10o P.C. = 1.670.04
3.xx PLT 10o P.C.
Red-Top Vacutainer
5 - 30
10o Equation:
7.00 RBC10o P.C. = 1.67 0.04
3.xx PLT10o P.C.
5 - 31
Voltage-Divider Fixture
R ed (+1 5V )
5K O H M S
100 O H M S
3 2 0 80
Digital Voltmeter
Verification/Adjustment Procedure:
1. Refer to Figure 5-6. Install the fixture on the MAM by
attaching the red lead to the top of L2, black lead to TP7,
and the yellow lead to TP8.
2. Attach the positive lead of the DVM to TP8 and the
negative lead to TP7.
Yellow (+10m v)
B lack (G N D )
5 - 32
TP 2
R84
3 2 0 81
R102
TP 11
R106
TP 9
TP 7
R137
TP 8
TP 1 7
R132
TP 1 5
L2
TP 1 8
R138
TP 2 1
R131
TP 1 6
5 - 33
Voltage-Divider Fixture
R ed (+1 5V )
5K O H M S
100 O H M S
3 2 0 80
Yellow (+10m v)
B lack (G N D )
Digital Voltmeter
Verification/Adjustment Procedure:
1. Refer to Figure 5-6. Install the fixture on the MAM by
attaching the red lead to the top of L2, black lead to TP7,
and the yellow lead to TP17
2. Attach the positive lead of the DVM to TP17 and the
negative lead to TP7.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
None
5 - 34
None
Verification/Adjustment Procedure:
1. From Main Menu, press
None
Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
TOWER TEST
2. Place a tube at the read station, and press
SPIN MOTOR
3. Verify that the tube is spun smoothly without any stopping
or jerking.
4. Press
DIAGNOSTICS
STOP MOTOR
MORE
to return to DIAGNOSTICS.
TOWER TEST
2. Alternately press Retract Solenoid and Extend
Solenoid.
This diagnostic routine first moves GS1 and GS2 down and
then up in the same manner as a count cycle. It then displays
the status of sensors GS1, S1, and S2 in both the up and down
positions (1=sensor blocked, 0=sensor unblocked). It also
displays the type of tube sensed at the aspirate position: BD,
SST, or No Tube.
5 - 35
BD Specimen Tube
Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
No Tube Example:
GS1 UP: Sensor Status
GS1=1 S1=0 S2=0
GS1 Down: Sensor Status Tube Type
GS1=0 S1=0 S2=1 No Tube
MORE
TOWER TEST
2. Place the appropriate tube at the vent/aspirate position.
3. Press
SENSOR TEST
GS1 DOWN
4. Verify the following sensor status and tube type for each
tube condition.
Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
BD Tube Example:
MORE
None
LOADER TEST
2. Alternately press MIXER DOWN and MIXER UP and
verify that the assembly moves up and down smoothly
without any stopping or jerking. Also verify that the home
sensor is 1 when up and 0 when down.
None
5 - 36
None
Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
LOADER TEST
2. Press
CYCLE BLADDERS
Specimen Tubes
Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
LOADER TEST
2. Place a rack with alternating tubes at the load side rear
wall.
3. Press
START RACK ADVANCE
4. Verify that the rack indexes smoothly through the
processing station without any stopping or jerking during
index movements.
5. Verify that when a tube is present at a sensor position a 1
is indicated, and when a tube is not present a 0 is
indicated.
5 - 37
5. Remove rack from the load side, place five racks in the
unload side, and press
EXTEND ARMS
Verify the following sensor status, and press RETRACT
ARMS.
5 Racks
SENSOR STATUS
Verification/Adjustment Procedure:
UNLOAD FULL
DIAGNOSTICS
UNLOAD WARN = 1
MORE
LOADER TEST
2. Leave the load side and unload side empty, and
alternately press EXTEND ARMS and RETRACT
ARMS.
3. Verify that the arms extend and retract smoothly without
any stopping or jerking.
4. Place one rack in the load side, leave unload side empty,
and press
EXTEND ARMS
Verify the following sensor status, and press
RETRACT ARMS
SENSOR STATUS
EXTEND ARMS:LOAD SENSOR = 0
UNLOAD FULL
=0
=1
5 Racks
Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
VER RACK BAR CODE
2. Manually move a rack through the processing station,
positioning the rack and tube position bar code labels at
the slot in the processing station.
3. Verify that the bar code number on the screen matches the
number on the rack.
UNLOAD WARN = 0
5 - 38
Pressure Gauge
Slotted Screwdriver
Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
VOLTAGE READINGS
2. Verify Press 1 psi is 12.5-13.5 psi. If out-of-range,
perform Vacuum & Pressure Level Verification/Adjustment
(VP-16).
3. Press
MORE
Verification Procedure:
1. Power off instrument using the proper shutdown
procedure.
2. Remove the shear valve driver from the Analyzer.
3. Lubricate the assembly as shown in Figure 5-9.
4. Re-install the shear valve driver and verify instrument
performance.
LOADER TEST
4. Press
CYCLE BLADDERS
5. Remove tubing from vacuum/pressure port on mixer head,
and connect tubing to pressure gauge.
6. Press
GRIP TUBE
to apply pressure.
5 - 39
F ront
3 2 0 82
Cotton Swabs
Verification Procedure:
1. Clean the left side and rear fan filters.
2. Remove Analyzer top cover and plastic optics covers.
3. Close Laser Shutter.
4. Using pressurized air, remove dust and dirt from optics
bench and surrounding area.
5 - 40
F orw ard
S lit
F ootball
S haped
B eam
N O R ed
H alo
3 2 0 84
Laser Tube
R ear
M irror
N O R ed
H alo
3 2 0 83
5 - 41
Figure 5-13
R ear
M irror
3 2 0 87
Insert C otton
S w ab H ere
3 2 0 85
Figure 5-14
Mirror Removal
Tape
R em ove
M irror
3 2 0 88
5 - 42
5 - Verification Procedures VP-44 Optical Flow Cell Cleaning & De-Bubble Procedure
c. Clean mirror using reagent grade methanol or
microscope lens cleaning solution (green) and high
grade lens paper. (Kodak recommended - DO NOT
USE Baxter S/P). Ensure surface is clean; use
compressed air to remove any remaining lint.
d. Replace mirror and align marks. DO NOT tighten
screws.
e. Open Laser Shutter.
DIAGNOSTICS
MORE
WBC DATA
EXTENDED WBC
COUNT
START
10 mL Syringe
Verification Procedure:
1. Fill syringe with 20% bleach solution or ISE Cleaning
Solution.
2. From Main Menu, press
n SPECIAL PROTOCOLS
n EMPTY/FILL FLOW CELL
n EMPTY FLOW CELL
5 - 43
Figure 5-15
DIAGNOSTICS
MORE
SOLENOID OPERATION
56
57
In je c t 20 %
B le a ch o r
IS E C le an in g
S o lu tio n
& Air a t
Th is P o in t
91
3 2 0 89
5 - 44
10 mL Syringe
11. Refer to Figure 5-16. Verify that there are no large bubbles
in the flow cell. If large bubbles are present, repeat this
procedure.
Figure 5-16
Verification Procedure:
SPECIAL PROTOCOLS
EMPTY/FILL FLOW CELL
EMPTY FLOW CELL
3. From Main Menu, press
DIAGNOSTICS
To W a ste /
Va cu u m
C e ll
S tre a m
N O L a rg e
A ir B u b b le s
Fe w S m a ll A ir
B ub b le s a re O K
MORE
SOLENOID OPERATION
4. To open solenoid 56, press
STEP SOLENOID
type 56, and press
ENTER
5. Refer to Figure 5-15. Remove elbow from right side of
solenoid 57, and connect elbow to syringe.
S he a th P rim e
S he a th In
3 2 0 90
S am p le In
5 - 45
Figure 5-17
5 MW
M o u n t C e n te re d
in B e a m Pa th
32091
Verification Procedure:
1. Ensure laser has been on for at least 15 minutes.
2. Perform Optics Bench Cleaning Procedure (VP-43).
3. Refer to Figure 5-17. Check laser power after first mirror:
a. Mount power meter photodiode centered in beam path.
b. Verify that power is 5 mW or greater.
c. If power is less than 5 mW, replace laser tube.
5 - 46
DI Water
Clean Container
3 2 0 92
Verification Procedure:
1. From Main Menu, press
SPECIAL PROTOCOLS
Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
VOLTAGE READINGS
2. Ensure top cover is in place, or cover optics bench with
black cloth.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
5 - 47
Loop
Rear
S he a r
Va lv e
B lo ck
C e n te r
Fron t
Loop
M o un tin g G u id e
K no b
R im N o tch
L o ck N otc h
20 mL Syringe
3 2 0 93
Verification Procedure:
1. From Main Menu, press
6. Clean mounting guide and lock notches in each ceramic
section.
SPECIAL PROTOCOLS
AUTO CLEAN
MORE
Follow the on-screen instructions to perform the
procedure.
2. Run three background counts to purge enzyme cleaner.
3. From Main Menu, press
DIAGNOSTICS
MORE
11. Press
VOLTAGE READINGS
5 - 48
H GB Flow Ce ll
Specimen Tubes
Diluent
Verification Procedure:
3 2 0 94
DIAGNOSTICS
MORE
4. Place a tissue under probe, run stylet into the top of probe
until it extends out the bottom, and wipe any material from
stylet. Run up and down several times and remove.
VOLTAGE READINGS
5 - 49
Specimen Tubes
Verification Procedure:
1. In the RUN mode use CHANGE SAMPLER to select
CLOSED SAMPLER.
2. From Main Menu, press
DIAGNOSTICS
MORE
TOWER TEST
5 - 50
None
Verification Procedure:
Verification Procedure:
1. Power-up the system and observe the 7-segment LED on
the CPU/DCM. Refer to the following table for status
conditions.
NOTE: After all the tests pass and solenoids energize, 1
and E will alternately flash in the display. This is a normal
condition and does not indicate a problem.
Table 5-6
None
Verification Procedure:
1. In the MAIN menu press
F12
F8
5 - 51
ENTER
ENTER
to run CHKDSK/F.
.
Verification Procedure:
1. In the MAIN menu, press
1 ,2 7 9 ,6 8 8 ,7 0 4 b y te s to ta l d is k sp a c e
1 3 1,0 7 2 b y te s in 2 h id d e n file s
F12
F8
2. At the C:\CD3200 prompt, type A: and press
3 2 , 7 6 8 b y te s in e a c h a llo c a tio n u n it
3 8 ,0 53 to ta l a llo ca tio n u n its o n d is k
16 ,0 5 7 a v a ila b le a llo ca tio n u n its o n d is k
6 5 5 ,3 6 0 to ta l b yte s m e m ory
6 1 8 ,5 6 0 b yte s fre e
Figure 5-21
None
ENTER
3. At the A:\> prompt, type CHKDSK/F and press
ENTER
4. At the selection prompt type Y and press
3 2 0 95
ENTER
to run CHKDSK/F.The display should closely resemble
Figure 5-22.
5. If there are no indications of a defect, type C: and press
ENTER
6. Then type rll and press
ENTER
to return to the MAIN menu.
5 - 52
WBC
(WOC)
NOC
RBC
HGB
PLT
5.0 k/L
Verification Procedure:
1. From Main Menu, press
None
Verification Procedure:
1. From Main Menu, press
RUN
SPECIMEN TYPE
BACKGROUND
2. Press the TOUCH PLATE to run a background count.
3. Verify that the background counts are within specifications
according to the following table.
RUN
SPECIMEN TYPE
2. Move the cursor to an empty file, and press
QC SPECIMEN
3. Mix the fresh blood thoroughly, and run 10 times in the
open mode and 10 times in the closed mode.
4. Press
MAIN
QUALITY CONTROL
VIEW QC LOG
5. Verify the CVs are within specification according to the
following table.
5 - 53
CV Specifications
WBC
NOC
(WOC)
RBC
HGB
MCV
PLT
MPV
d. Press
CELL-DYN Calibrator
f.
Verification Procedure:
ACCEPT
g. Press
1. Pre-calibration checks:
START AUTO-CAL
CALIBRATION
AUTO-CALIBRATE
DI Water
#2 Phillips Screwdriver
5 - 54
TO
FL O W PA N E L
9P SI
DRAIN ACCUMULATORS
9. Initialize Analyzer and repeat Step 8.
32004
Figure 5-23
MORE
12P SI
To S L
To S L
Vacuum/Pressure Manifold
DI Water
#2 Phillips Screwdriver
Verification Procedure
1. Initialize Analyzer, and remove Left Side Cover.
2. Extend Air Supply out of Analyzer (Vacuum/Pressure
Assembly (RR-7)).
5 - 55
TO
FL O W PA N E L
9P SI
12P SI
To S L
To S L
32004
Figure 5-24
Vacuum/Pressure Manifold
DIAGNOSTICS
MORE
IDRAIN ACCUMULATORS
9. Initialize Analyzer and repeat Step 8.
Verification Procedure
1. Perform (VP-64) Backup Procedure .
2. Turn System OFF.
3. Insert Service Disk into Floppy Drive.
4. Turn System ON.
5. At A:\ prompt type CHKDSK/F, then press
ENTER
5 - 56
ENTER
None
Verification Procedure
3 2 , 7 6 8 b y te s in e a c h a llo c a tio n u n it
3 8 ,0 53 to ta l a llo ca tio n u n its o n d is k
16 ,0 5 7 a v a ila b le a llo ca tio n u n its o n d is k
Figure 5-25
6 5 5 ,3 6 0 to ta l b yte s m e m ory
6 1 8 ,5 6 0 b yte s fre e
3 2 0 95
DIAGNOSTICS
b. Press
F12
F1
RETURN
Verification Procedure
1. Turn System OFF.
2. Insert Service Disk into Floppy Drive.
CELL-DYN 3200 SYSTEM SERVICE MANUAL
5 - 57
Verification Procedure
Verification Procedure
1. Turn System OFF.
2. Insert Setup Disk.
Verification Procedure
1. Collect Data with List Mode ON.
a. From MAIN MENU, Press
DIAGNOSTICS
b. Press,
F12
F1
STORE RAW ON/OFF (To ON)
c. Press,
RETURN
RUN
5 - 58
Press
MAIN
DIAGNOSTICS
g. Press,
F12
F1
F12
F8
ENTER
ENTER
ENTER
to get listing of all files in RAWFILE directory.
c. Select files to be deleted.
d. Type DEL C:\RAWFILE\(FILE NAME) for each file to
be deleted.
4. Press
RETURN
Verification Procedure
1. Turn system power OFF.
2. Insert disk #1 in the floppy drive.
3. Turn system power ON.
4. When instructed, insert disk #2.
5 - 59
NOTE: 0 = zero
a. Press F8 to exit to the C:\CD3200> prompt.
b. Turn off analyzer, wait 5 sec, turn on analyzer to
reboot.
5 - 60