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5. Action of fuchsine. Sulphites reduce dyes (fuchsine, malachite green). At sulphites presence
disappears dye colouring. Fuchsine thus forms fuchsine-sulphite acid a colourless salt of Nsulphine acid of p-fuchsineleucosulphoacid (at pH 3-6):
_
+ .
NH2 Cl
H2N
H3C
+ 2Na2SO3 + HCl
NH2
H2N
NH-SO2-Na
H3C
+ 2NaCl + 2H2O
NH2-SO2-Na
OH
1. Action of barium chloride. Oxalate-ions with barium ions forms white crystalline precipitate:
(NH4)2C2O4 + BaCl2= BaC2O4 + 2NH4Cl
Precipitate is soluble in boiling acetic acid (difference from calcium oxalate) and in solutions of
strong acids with formation of oxalic acid.
2. Action of calcium chloride. Calcium chloride with oxalates forms white small-crystalline
precipitate of calcium oxalate, soluble in mineral acids and insoluble in acetic acid:
(NH4)2C2O4 + aCl2= aC2O4 + 2NH4Cl
3. Action of potassium permanganate. Potassium permanganate in acidic environment with
oxalate-ions at heating is discoloured as a result of reducing to Mn+2:
5(NH4)2C2O4 + 2KMnO4 +8H2SO4= 2MnSO4 + K2SO4 + 10CO2 + 5(NH4)2SO4 + 8H2O
3. Flame test. The volatile boron compounds (H3BO3, BF3, H3BO3 ethers) paint gas-ring flame in
green colour. Concentrated sulphate acid and ethanol (or methanol) acting on dry sodium borate
forms boron-ethylic (boron-methylic) ether, which gives to flame character green colour:
H3BO3 + 3H3OH = B(OCH3)3 + 3H2O
OH
OH
HO
OH
1. Action of iron(I) sulphate and hydrogen peroxide. Iron() hydroxide with tartrates forms dark
blue complex compounnd:
HO
OH
OH
O
O + 2H2O
O
OH + Fe(OH)3
HO
O O
OH
Fe
OH
2. Action of resorcinol in acidic environment. Crystals of tartrate acid salt with resorcinol or
pyrogallol at presence of concentrated sulphate acid and at heating give a violet product of
condensation of resorcinol or pyrogallol with glycol aldehyde, which forms from tartrate acid
under action of sulphate acid:
O
HO
OH
+ H2SO4
OH +
HO
OH
OH
HO
O
HO
Structural formula of citrate acid:
OH
OH
1. Action of calcium chloride. Calcium chloride with salts of citric (lemon) acid forms soluble in
acids white precipitate:
H3657 + CaCl2 CaHC6H5O7 + 2HCl
2. Action of acetic anhydride. Acetic anhydride and citrates at heating with concentrated sulphate
acid form acetylsalicylate with character smell:
HO
OH
H3C
OH
O
+
HO
O
OH
O
O
OH
H2SO4
H3C
+ CH3COOH
O
OH
H3C
+ Cl2
N N
H H
O
N N
N N
H H
+ 2HCl
O
Reaction performance. Investigated substance place in test tube, add 0,2 g of potassium
dichromate and 1 ml of sulphate acid. The test tube opening cover a filtration paper impregnated
with 0,1 ml of diphenylcarbazide solution. A paper is painted in the violet-red colour.
2. Action of potassium permanganate. Strong oxidants in acidic environment oxidise chlorides to
free chlorine:
2KMnO4 + 10KCl + 8H2SO4 = 2MnSO4 + 5Cl2 + 6K2SO4 + 8H2O
Free chlorine detects by smell or by turning in blue colour of iodine-starch paper, which contains
and starch.
NH-SO2-Na
H3C
+ Br2 + 2H2O
NH-SO2-Na
+
_
NH2 . Br
H2N
H3C
+ 2NaHSO4 + HBr
NH2
AgNO3 + K = Ag + KNO3
2. Action of lead salts. Lead salts with iodides form yellow precipitate, which re-crystallise from
water and as gold like scales:
Pb(NO3)2 + 2K = PbI2 + 2KNO3
3. Action of oxidisers. Solutions of potassium dichromate and potassium permanganate in
environment of sulphate acid, and sodium nitrite in acetic environment oxidize iodides to free
iodine:
K2Cr2O7 + 6K + 7H2SO4 = Cr2(SO4)3 + 32 + 4K2SO4 + 7H2O
2KMnO4 + 10K + 8H2SO4 = 2MnSO4 + 52 + 6K2SO4 + 8H2O
2HI + 2HNO2 = I2 + 2H2O + 2NO
Oxidization of iodide-ions by nitrites in environment of acetic acid is specific and distinguishes
iodides from chlorides and bromide. Free iodine detects by turn in blue of starch solution.
Chloroform is painted in violet colour.
4. Action of mercury() nitrate. Iodides are precipitate from solutions of mercury() salts with
formation of brown-green colour sediment:
1. Action of iron(III) chloride. Iron() chloride in neutral environment with benzoates forms
yellow-pink precipitate:
COO-FeCl2
COONa
+ NaCl
+ FeCl3
2. Action of concentrated sulphate acid. Investigated substance places in test tube and wets by 0,2
0,3 ml of concentrated sulphate acid, carefully heat a bottom of test tube; the white raid appears
on inner walls of the test tube.
COOH
+ H2SO4 conc.
- H2 O
+ CO2
COONa
COOH
+ HCl
+ NaCl
12
NH
+ NaNO3 + H2SO4
2. Reaction of brown ring formation with iron(II) sulphate. Iron sulphate in acidic environment
reduces nitrates to nitrogen(II) oxide:
2NaNO3 + 6FeSO4 + 4H2SO4 = 3Fe2(SO4)3 + Na2SO4 + 2NO + 4H2O
The nitrogen() oxide forms with FeSO4 brown complex [FeNO]SO4:
FeSO4 + NO = [FeNO]SO4
Reaction performance: investigated solution and concentrated sulphate acid carefully layer on
FeSO4 crystals. The brown ring thus appears.
3. Reaction with metallic zinc and concentrated solution to sodium hydroxide. Zinc, magnesium,
and aluminium in alkaline environment reduce nitrate-ions to ammonia, which turn colour of
litmus paper from red to blue:
NaNO3 + 4Zn + 7NaOH + 6H2O = NH3 + 4Na2 [Zn(OH)4]
4. Reaction with antipyrine. In environment of concentrated sulphate acid nitrates with antipyrine
form red colour nitroantipyrine:
H3C
H3C
NO2
+ NaNO3 + H2SO4
H3C
N
C6H5
+ Na2SO4 + H2O
H3C
C6H5
H3C
H3C
NO
+ NaNO2 + H2SO4
H3C
+ Na2SO4 + H2O
H3C
C6H5
C6H5
2. Action of mineral acids. Mineral acids liberate nitrite acid from nitrites:
2NaNO2 + H2SO4 = 2SO4 + 2NO2
Nitrite acid is unstable and decomposes with formation of nitrogen oxides and water:
2NO2 = NO2 + NO + H2O
3. Action of potassium permanganate. Potassium permanganate in acidic environment oxidiszes
nitrites to nitrates; thus solution of potassium permanganate is discoloured as a result of Mn2+
ions formation:
5NaNO2+ 2KMnO4 +3H2SO4 = 2MnSO4 + K2SO4 + 5NaNO3 + 3H2O
4. Reaction of brown ring formation with iron(II) sulphate. Iron sulphate in acidic environment,
like nitrates, reduces nitrites to nitrogen(II) oxide:
2NaNO2 + 2FeSO4 +2H2SO4 = Fe2(SO4)3 + Na2SO4 + 2NO + 2H2O
Nitrogen() oxide with FeSO4 forms brown complex [FeNO]SO4:
FeSO4 + NO = [FeNO]SO4
Reaction performance: explored solution and concentrated sulphate acid carefully layer on FeSO4
crystals. The brown ring thus appears.
5. Action of potassium iodide. Potassium iodide in acidic environment reduces nitrites to
nitrogen() oxide, oxidizing thus to free iodine, which turns water solution to brown or starch
solution to blue:
2KNO2 +2KI +2H2SO4 = I2 + 2K2SO4 + 2NO + 2H2O
6. Action of diphenylamine. Diphenylamine reduces nitrites, like nitrates, oxidizing thus to
diphenylbenzidine, and then to dark blue compound:
NH
+ NaNO2 + H2SO4
+ Na2SO4 + NO + H2O
14
7. Reaction with sulphanylic acid and - or -naphthole (Griss test). Sulphanylic acid with
sodium nitrite in acidic environment forms diazonium salt, which enters in reaction of azoconjugation with naphtholes. In this reaction bright red azo-dye forms:
N N
NH2
Cl
+ NaNO2 + HCl
+ NaCl + H2O
SO3H
SO3H
OH
+
N N
OH
N N
+ NaCl + H2O
Cl +
SO3H
+ NaOH
SO3H
15
OH
1. Action of iron(III) chloride. Iron() chloride with salicylate-ions forms iron() salicylate
having indigo or red-violet colour (depending on composition).
FeCl
COO
COOH
OH
+ 2HCl
+ FeCl3
Colouring disappears in case of chloride acid addition (formation of precipitate of salicylic acid)
and is saved in case of acetic acid addition.
2. Action of chloride acid. Chloride acid from salicylates solutions deposits white precipitate,
which after re-crystallisation from the hot water and drying in vacuum has a melting point from
156 C to 161 C:
COONa
COOH
OH
OH
+ HCl
+ NaCl
COOH
OH
OH
+ CH3OH
H2SO4 conc.
+ H2 O
16