Professional Documents
Culture Documents
(BLOOD)
Class of 2016 Section C
CELL TYPES
ERYTHROCYTES (RBC)
biconcave shape
center often look
lighter than their
periphery
LEUCOCYTES
(WBC)
Granulocytes
(Neutrophil, Eosinophil, Basophil)
GENERAL CHARACTERISTICS
Polymorphonucleated with a two to eight lobed
nucleus.
Lobes of the nucleus are connected by thin
strands of chromatin.
Cytoplasm is acidophilic and contains specific
granules. These granules have a size and stain
affinity which allows the granulocytes to be
placed into categories.
Also referred to as myeloid cells due to their
exclusive origin from the bone marrow.
NEUTROPHIL
Barr body
NEUTROPHIL
3 types of granules:
o Primary granules
(azurophilic granules)
o Secondary granules
(specific for
neutrophils)
o Tertiary granules
(contain enzymes(
EOSINOPHIL
Have a bilobed nucleus
The granules are richly
eosinophilic (acidophilic).
About 12 to 17um in
diameter.
Rare circulating leukocyte
(1-6%)
Their functions are
associated with allergic
reactions and parasitic
infections.
EOSINOPHIL
BASOPHIL
2-3 nuclear lobes (not well defined)
Specific granules of basophils are
stained deeply bluish or reddishviolet
About 12 to 15 um in diameter.
Less than 1% of the circulating
leukocytes
They have a purely secretory
function. The contents of their
granules function in inflammatory,
immune and hypersensitivity
reactions
Contains heparin, histamine,
lysosomal enzymes and
leukotrienes
BASOPHIL
LEUCOCYTES
(WBC)
Agranulocytes
(Lymphocytes, Monocytes)
GENERAL CHARACTERISTICS
Mononuclear leucocytes (non-lobulated
nuclei)
Cytoplasmic granules were not readily seen
with early microscopic methods (give the
erroneous impression that these cells are
devoid of cytoplasmic granules)
LYMPHOCYTE
Nucleus is round and surrounded
by a narrow rim of cytoplasm
Second most numerous leukocyte
in the circulation (25%).
Smallest cells in WBC series
Functionally, the lymphocytes fall
into two categories:
o B lymphocytes responsible
for antibody formation
o T lymphocytes - responsible
for contact destruction of
foreign tissues,
hypersensitivity reactions and
cancer cell detection and
destruction.
LYMPHOCYTE
MONOCYTE
Large cells, about 20 um in
diameter.
Nucleus is off center and
indented, C-shaped, or
kidney-shaped.
Voracious phagocytes and
become highly activated
after making contact with
foreign antigen.
About 2-10% of the
circulating leukocytes
Plays a crucial role in
inflammatory and immune
responses.
MONOCYTE
HAEMOPOIESIS
Erythropoiesis
Granulopoiesis
Thrombopoiesis
Monopoiesis
Lymphopoiesis
ERYTHROPOIESIS
Red Blood Cell Formation
PROERYTHROBLAST
Pronormoblast
Cytoplasm
o Abundant RER
o Light blue to deep
clumps in pale grayish
blue background
Nucleus
o 80% of cell; spherical,
center
o Fine chromatin network
o Burgundly red nucleus
with nucleoli
BASOPHILIC NORMOBLAST
Basophilic Normoblast or Early
Normoblast
Has chromatin, clock-face pattern
Hb already synthesized but
cytoplasm is still blue
Cytoplasm:
o Highly basophilic
o Bluish clumps in pale blue
cytoplasm
Nucleus:
o 75%
o Coarse chromatin; Clock-face
pattern
POLYCHROMATIC NORMOBLAST
Intermediate Normoblast
Cytoplasm
o significant
Hemoglobin ->
grayish pink
Nucleus
o 50% - Checkerboard
chromatin; no
nucleulos
ORTHOCHROMATIC NORMOBLAST
Late normoblast
Low nucleus-cytoplasmic
ratio: 25%
Cytoplasm
o Pinkish with a slight
tinge of blue
o Paucity of degenerating
organelles
Nucleus
o Eccentric, pyknotic
(heterochromatic)
o In process of being
extruded
RETICULOCYTE
Prematurely enters
blood & complete cycle
Cytoplasm
o Bluish reticulum is
evident (due to rER)
o Looks like RBC but
have different
colors, with the
presence of dots
Nucleus: Absent
REMEMBER!
Cell size can aid in the
identification of the
different stages of
erythropoiesis.
P - Proerythroblast
B - Basophilic
Erythroblast
L - Polychromatic
Erythroblast
O - Orthochromatic
Erythroblast
E - Erythrocyte
GRANULOPOEISIS
Granulocyte Formation
MYELOBLAST
Earliest recognizable stage
Pre-cursor of all granulocyte
lineage
Cytoplasm
o Scanty; moderately basophilic
o NO GRANULES
o no ducts
o small blue clumps in lt. blue
background
o cytoplasmic blebs
Nucleus
o Round, reddish blue
o Lumped & coarser chromatin
o Capable of mitosis
NEUTROPHIL LINEAGE
NEUTROPHILIC PROMYELOCYTE
Cytoplasm
o contains azurophilic
granules
Nucleus
o slight chromatin
condensation
o less prominent nucleoli
Normally, they are not present
in the peripheral blood.
NEUTROPHILIC MYELOCYTE
Cytoplasm
o More abundant
o Contains secondary granules
o Denser Azurophilic granules
o More granules seen at the
periphery
Nucleus
o Round-oval, often flattened
on one side
o Cromatin: clumping, coarse;
no indistinct nucleolus
Normally, they are not present in
the peripheral blood.
NEUTROPHILIC METAMYELOCYTE
Same size as neutrophil
Cytoplasm
o Fewer azurophilic granules
o Many secondary granules
Nucleus
o Kidney-shaped
o Relatively dense chromatin
specially along the nuclear
membrain
Neutrophil
Neutrophilic
Stab Cell
Neutrophilic
Metamyelocyte
Neutrophilic
Myelocyte
EOSINOPHILIC LINEAGE
There are stages na wala kong mahanap na description. And medyo sabog ung
Eosinophilic and Basophilic Lineage. Sorry. Yan lang ung inabot ng time and
research powers ko. :\ Godbless sa pag-aaral ng part na to.
EOSINOPHILIC PROMYELOCYTE
This stage was not included in Dr. Tolentinos lecture based on the upper batch transcription.
EOSINOPHILIC MYELOCYTE
Cytoplasm
o With spherical eosinophilic
granules that are larger
compared to those of neutrophils
o Eosinophil granules pack the
cytoplasm and stain reddishorange
o May have some granules with
basophilic staining characteristics
Nucleus
o Oval and sometimes has a slight
indentation in one side
Not typically seen in peripheral blood
EOSINOPHILIC METAMYELOCYTE
Neutrophil Band Form
Cytoplasm
o Granulated
Nucleus
o Forms lobes
This stage was not included in Dr. Tolentinos lecture based on the upper batch transcription. In
addition, based on the transcription Eosinophilic lineage does not have a stab cell/band form stage.
BASOPHILIC LINEAGE
BASOPHILIC PROMYELOCYTE
This stage was not included in Dr. Tolentinos lecture based on the upper batch transcription.
BASOPHILIC MYELOCYTE
Similar to Eosinophilic
Myelocyte but
cytoplasm more
basophilic
BASOPHILIC METAMYELOCYTE
Similar to Eosinophilic
Metamyelocyte but
cytoplasm more
basophilic
THROMBOPOIESIS
MEGAKARYOPOIESIS
Platelet Formation
MEGAKARYOBLAST
Cytoplasm
o Homogenous
o Intensely basophilic due to
numerous ribosome
Nucleus
o Slightly indented
o Loose chromatin,
inconspicuous nucleoli
Undergo Endomitosis
(incomplete type of mitosis:
no cytokinesis)
MEGAKARYOCYTE
Cytoplasm
o Lesser basophilic
o With azurophilic granules
Nucleus
o Multilobulated
o Coarse Chromatin
PLATELET
Smallest particle in
the blood
Lack nuclei
Consist of basophilic
cytoplasm and tiny
azurophilic granules
MONOPOIESIS
Monocyte Formation
LYMPHOPOIESIS
Lymphocyte Formation
MONOBLAST
Decreased
Nucleus:Cytoplasm
Ratio
Scanty cytoplasm
with Azurophilic
granules
Resembles
myeloblasts but
nucleus is more
indented
PROMONOCYTE
Cytoplasm
LYMPHOBLAST
Large cell, spherical
Nuclear: Cytoplamic Ratio: 6:1
Cytoplasm
o No azurophilic granules
Nucleus
o Large nucleus
o Visible nucleoli
o Highly dispersed chromatin
PROLYMPHOCYTE
Slightly smaller than
lymphoblast
Presence of azurophilic
granules
Denser, coarser
chromatin
ADDITIONAL PHOTOS
1. Promyelocyte
2. Metamyelocyte
3. Eosinophilic metamyelocyte
4. Monocyte
1. Promyelocyte
2. Metamyelocyte
3. Band form
1. Metamyelocyte
2. Promyelocyte
1. Myeloblast
2. Promyelocyte
3. Eosinophilic myelocyte
4. Lymphocyte
1. Plasma cell
2. Promyelocyte
3. Pronormoblast
1. Metamyelocyte
2. Orthochromatic normoblast
3. Polychromatic normoblast
3. Polychromatic normoblast
4. Plasma cell
Most of the cells from this stage are neutrophilic since neutrophils are the most
numerous type of mature granulocyte. Cells in the myelocyte stage may also have clear
halo bordering the indentation of the nucleus representative of an extensive Golgi
apparatus.
Book Reference
Young, B. Heath, J. (2001). Wheaters
Functional Histology (4th Ed.). USA: Churchill
Livingstone.
Online Quizzes
http://www.biomed.ucf.edu/histology/quizzes
/published/Quiz8b-Hematopoiesis.html
http://www.histologyworld.com/quizlinks/quizlinks11j.htm
http://www.histologyworld.com/quizlinks/quizlinks8a.htm
http://medinfo.ufl.edu/year1/histo/quiz/mh1
0.html
FIN.
Godbless everyone! :D