Professional Documents
Culture Documents
8/23/2004
What are the 6 steps in the infection
1. encounter
process?
2. entry
3. spread
4. multiplication
5. damage
6. outcome
What is penetration? Example?
When the microbe enters and gets past the
epithelium. CUT?
What is ingress? Example?
When the microbe enters the body but must still
pass through epithelium? Swallowing!
When does the spread of the microbe
When the microbe successfully beat down the bodies
occur?
defenses.
What 3 things does the spread of a
1. morphology of the host
microbe depend on?
2. morphology of the microbe
3. time
How does a microbe become infectious? Multiply
What does the host do in response to
In most cases another type of defense mechanism is
the microbes multiplication.
initiated (ex lymphocytes). The microbe must be able
to overcome this defense mechanism to successfully
become infectous.
What are the 3 ways the microbe
1. evolution
survives the hosts initiated defense
2. protective coats
mechanisms when trying to multiply?
3. release a toxin
T/F: Release of a toxin by a microbe is
False, release of toxins are rare most must
the most common response.
multiply to become infectious.
Chapter 2 Reading
To what group do Bacteria, fungi, and
Protists
protozoa belong to?
How do bacteria reproduce?
Binary fission
How do viruses reproduce?
The must be located within a host cell where they
disassemble, make copies and reassemble as a new
generation.
What is the shape of a bacterium
The rigid cell wall.
determined by?
What are the three classifications of
1. cocci spherical
bacteria by shape? What is the shape of
2. bacilli rod
each?
3. spirochaetes helical
What is the bacterium classification
Pleomorphic
when it can have many different
shapes?
What is the range of bacterial size?
.2 micrometers to 5 micrometers
*Study table 2.1 and 2.2
What are the two classification of
1. gram-positive purple
bacterium by gram staining? What color
2. gram-negative pink
is each?
remember the nomonic???????
*Which type of bacterium is more
Gram positive is more suseptable to penicillin than
susceptible to penicillin?
gram-negative
(NOTE: HIV positive have to take penicillin)
Are viruses or bacteria visible with a
bacteria
light microscope?
Bacteria
Bacteria
Flagellum
flagellin
Hairlike extensions from the cell surface similar to
flagellum but smaller.
Gram-negative
1. mediate the adhesion of bacteria receptors to
human cells.
2. Reproduction
Sex pilus
It is a necessary first step for infection.
It is a slime layer.
Polysaccharides
The glycocalyx allows the bacteria to adhere firmly
to other structures.
Strep mutans this is why caries are more rampant
in a diet high in sugar.
Polysaccharides
Each species has a different type of polysaccharide.
This helps in identification of each one.
1. mediates the adhesion of bacterium to human
tissue which is required for colonization and
infection
2. hiders or inhibits phagocytosis which helps it
overcome the bodies defenses and be more
virulent.
3. identification
4. *used in vaccines
It makes it rigid.
True
False, the cell wall is pourous and permeable to
substances of low molecular weight.
Peptidoglycan
A peptide and a sugar.
1. thickness
2. composition
Both gram positive and gram negative. However, it
is much thicker in gram postitive bacteria.
1. lipopolysacchrides
What is a mesosome?
What type of DNA replication does
bacteria undergo?
What is the ribosomal unit organization
of bacteria? Eukaryotes?
What are cytoplasmic inclusions?
2. lipoprotein
3. phospholipids
(rem. Three lipids are on the outside)
Porins, transport hydrophilic materials across the
membrane.
Outer membrane and the cytoplasmic membrane of
gram negative bacteria
It is where enzymes that kill drugs are located.
Endotoxin is located in the lipopolysaccharide of the
outer layer of the gram negative bacteria
Fever, shock, etc, during disease
NO, they do not have lipopolysaccharides
Bacteria that do not respond to the stain.
Yes, some can
1. mycoplasmas
2. L- form
3. spheroplasts/protoplasts
Spheroplasts/protoplasts
Lform and spheroplasts/protoplasts.
1. capsule
2. peptidoglycan
3. cytoplasmic membrane
4. cytoplasm
1. capsule
2. outer membrane
3. lipoprotein
4. peptidoglycan
5. periplasmic space
6. cytoplasmic membrane
7. cytoplasm
Cytoplasmic membrane, periplasmic space
Close but not. In eukaryotes the cytoplasmic
membrane contains sterols. In prokaryotes it
contains hopanoids
1. active transport of molecules into cell
2. passive diffusion throught the semipermeable
membrane
3. energy generation by oxidative
phosphorylation
4. synthesis of cell wall precursors
5. secreation of enzymes and toxins
It is the location at which the invagination occurs
during mitosis. It is also the attachment point of the
DNA for the two daughter cells.
Semiconservative bidirectional from a fixed point.
70S, 80S
Areas where stored energy is located.
1. polymetaphosphate
2. polysaccharide
3. B - hydroxybutyrate
1. baccilis
2. clostridium
When nutrients are scare.
Inert, may lie dormant for years
When the nutrients that were deprived are again at
appropriate levels for metabolism to occur.
Sterilization is necessary to kill them, disinfection is
not enough due to their ability to survive in extreme
circumstances.
1. polysaccharides
2. protein
3. lipid
4. nucleic acid
5. peptidoglycan
Balanced growth
1. autotrophes Use CO2
2. heterotrophs require complex sugars as
carbon source.
Carbohydrates
Amino acids
Growth.
Binary fission
Logarithmic
1. Lag phase
2. log phase
3. *stationary phase
4. decline/death phase
Maximal cell density is achieved during the
stationary phase and is the amount of bacteria that
can live under the current environmental conditions.
Nutritional environment
1. intercellular
2. extracellular
1. end product inhibition
2. catabolite repression
1. temperature
2. ph
1. mesophiles body temperature
2. thermophiles - > body temp
3. psychrophiles - < body temp
1. obligate aerobes
2. facultative aerobes
Describe plasmids
***In what type of gram stained
bacteria do plasmids exist?
T/F: only one plasmid occurs within a
cell?
Describe the two types of plasmids?
3. obligate anaerobes
4. microaerophiles
It has a single DNA strand and attached to the cell
membrane of the organism.
Semiconservate
Each daughter bacteria has one strand of DNA from
the parent cell and one newly synthesized strand.
DNA-dependant DNA polymerase
Mutation
1. base substitution mutation a base was
changed in the strand
2. frame shift mutation a base was added or
deleted.
3. insertion mutation a piece of foreign DNA
was inserted into the DNA
1. missense mutation the mutation codes for
another amino acid.
2. nonsense mutation the mutation codes for a
stop codon and the rest of the DNA and
causes the premature termination of protein
synthesis.
1. conjugation
2. transduction
3. transformation
4. transposition
This is how bacteria pass on antibiotic resistance.
Recombination is when transferred DNA by one of
the above methods is integrated into the host DNA.
1. Homologous Recombination two strands that
have extensive homologous regions exchange
pieces (ie during mitosis)
2. non-homologous Recombination little
homology exists.
Extrachromosomal DNA that are capable of
replicating independent of the recipient DNA.
Both
False
1. transmissible the DNA fragment have a
transfer gene and can be transferred by
conjunction.
2. non-tranmissible the DNA fragment do not
have a transfer gene and must accompany
another plasmid with a transfer gene inorder
to be transferred.
Bacteria that have a plasmid.
1. antibiotic resistance
bacteria?
2. production of colicins
3. resistance to heavy metals including Hg.
4. pili help with adherence to epithelial cells
5. exotoxins
Transposons
The can not replicate independently of the recipient
DNA.
When they are transferred, it is a copy of the
tranposon that is transferred. The original transposon
remains in the recipient DNA.
Gene cloning
membrane structures.
What is a hopanoid?
Why are hopanoids important?
**What are the 4 functions of a plasma
membrane?
1. 1
2. 2
Inbetween them
Gram Positive!!!!
In between the two plasma membranes. (note: inner
plama membrane periplasmic space cell wall
outer plasma membrane
1. Lipid A protein
2. core phospholipids
3. O side chain
O antigen!
1. stabilizes the outer membrane
2. acts as an endotoxin
Contributes to the negative charge of the cell
membrane
Protection from host!!
Polysaccarides
False, capsules are well organized and hard to
remove.
True
They are a component of Strep mutans.
Chains of polysaccharides.
protection from host defenses
protection from harsh environmental
conditions
attachment to surfaces
protection from viral infection or predation by
bacteria
protection from chemicals in environment
motility of gliding bacteria
Protection against osmotic stress.
Cellular adhesion
G+ flagellum only go to the plasma membrane. Gflagellum are attached to both plasma membranes.
It is formed by some bacteria to protect itself from
harsh conditions. (ie nutrition?)
No
The uptake of naked DNA into the bacterial cell.
1. integration
2. degradation
The plasmid is not integrated. It just remains in the
cytoplasm. However, it can still be degraded.
DNA binding proteins
Energy
*Describe papovavirses.
*Describe papillomavirus
*Describe Polymavirus
*Describe Adenoviruses
*Describe Herpesviruses
*Describe Poxviruses
*Describe parvoviruses
*Describe Hepadnaviruses
*Describe Picornaviruses
*Describe Orthmyxoviruses
*Describe Paramyxoviruses
*Describe retroviruses
*What is a viroid
*T/F: viroids are pathogenic to humans?
small
icosahedral
produce tumors in vivo
contains human serotypes which cause benign
skin tumours or warts.
Involved in genital and oral cancers
From rats and monkeys
Used as carcinogen in studies
Isohedral
Associated with respiratory and eye infections
in humans.
Isolated from adenoid tissue which gives rise
to name
Viral cause of oral infections in humans
Recurrent
Latent
isohedral
Larges viruses to infect humans or animals
Icosahedral
Found in three places (stool, blood, and
synovial tissues of rheumatoid arthritis
patients.
Small
Spherical
Cause hepatitis , liver infections, liver cancer
Important in dentistry due their transmission
via saliva and blood.
Smallest of RNA viruses
Large group
Three types of enteroviruses (oliviruses,
echoviruses, and coxsackieviruses)
Reside and multiply in the gut
asymptomatically
Tubular nucleocapsid and a lipoprotein
envelope
Influenza A
Large
Pleomorphic enveloped RNA viruses
Contains measles, mumps, parainfluenza, and
respiratory syncytial viruses
Large
Spherical
Unique genome
Unique ezzyme
Unique mode of replication
3 subdivisions (lentiviruses (hiv), oncoviruses,
and spumaviruses)
They are the smallest known agents of disease.
False, they are pathogenic to plants.
*Describe penetration/uptake.
*What are the 3 mechanism of
penetration?
Describe transcription
1. adsorption
2. penetration
3. uncoating and eclipse
4. transcription
5. synthesis of viral components
6. assembly
7. release of virions
This is the period between infection an the
production of a new virion.
attachment of the virus particle to the specific
receptors of the host cell plasma membrane
cell must have receptors for virus for firm
attachment to occur
The process by which the virus or its genome
enters the host cell cytoplasm
Endocytosis take into cell
Fusion direct fusion of envelope into plasma
membrane
Translocation pass directly through plasma
membrane
Those viruses that do not have an envelope.
8/27/2004 Lecture
Viruses/cellular organism: have the
most complex structure?
Cellular organisms
Chapter 5
What is the term for a microorganism
that is capable of causing disease?
What is an opportunistic organism?
How are opportunistic microbes
transmitted into the body?
What is the quantitative measure of
pathogenicity?
*What two things is virulence related
to?
*What two ways is virulence measured
and how are they specified?
world wide?
*Describe the four stages of an acute
infection?
1. transmission
2. adherence to host surfaces
3. invasiveness
4. toxigenicity
Exogenous
Endogenous, inside the body
1. inhalation airborne route
2. ingestion from food and water
3. inoculation evasive
1. skin
2. respiratory tract
3. gastrointestional tract
4. genitourinary tract
Adherence to the host.
Some have developed adherence mechanisms.
It will not be infectious
Secretion of enzymes.
colaenase/hyaluronidase
coagulase
immunoglobulin A
Lucocidins
They degrade intercellular substances that allow
easy spread of the bacteria.
Accelerates the production of a clot that protects the
virus.
Degrades protective IgA antibodies on mucosal
surfaces allowing adherence to epithelial cells.
They destroy both neutrophilic leucocytes and
macrophages.
IL-1
TNF
fever
hypotension
alternative pathway of the complement
cascade
4. activation of the coagulation system
5. increased phagocyte activity
6. increase antibody production
Alternative pathway of the complement cascade.
Hypotension
Fever
Activation of coagulative system
Both gram pos and gram neg
They can cause disease in distant parts of th body.
Systemic system or diffusion
Polypeptides
1. binding to the cell membrane and entry into
the cell
2. production of toxin
Yes
They induce the synthesis of protective antibodies.
1. neurotoxins
2. enterotoxins
3. miscellaneous exotoxins
An endotoxin who is mediated through the neuronal
pathway!
Toxins that act on the gastrointestinal system.
Pathogenisis of viruses
No difference
inhalation
ingestion
inoculation
1. accidental abrasions or needle stick injuries
2. arthropod vectors
3. deep inoculation into the subcutaneous tissue
and muscle
1. desquamation of epithelium
2. presence of saliva and mucous layers
3. mechanical movements in mouth and
peristalsis.
4. immune mechanisms
1. secretion of mucous by goblet cells
2. IgA present in repiratory secretions
3. alveolar phagocytic cells
They need to be primarily aerosol or particulate in
form.
1. natural mucosal desquamation
2. vaginal secretions and cerical mucus.
3. intermittent flushing action of urine
True
1.
2.
3.
4.
passage?
*What are the four possible routes of
viral transmission in peripheral nerves?
*What are the two methods of virus and
host cell interactions?
*What is a permissive infection?
*What is a non-permissive infection?
*What are 4 ways a virus-infected cell
may die?
2. centrifugal
1. axon
2. endoneural cell (shwann cell)
3. connective tissue space between nerves
4. perineural lymphatics
1. permissive infection
2. non-permissive infection
An infection where there is synthesis of viral
components.
And infection that results in cell transformation often
with the integration of viral DNA into the host
genome.
1. shut down of host cell protein and nuclic acid
synthesis
2. cell lysis by the release of progeny virons.
3. intracellualar release of lysosomal enzymes
4. damage to cell membranes
1. haemadsorption
2. giant cell formation
Occurs when a common envelope protein enables an
infected cell to attract red cells at its surface.
Fusion of cells to make one giant multinucleated cell.
True
An infection where viral replication is occurring but
not at the expense of the cell.
1. low pathogenicity of the virus
2. ineffective host immune responses
3. no interferon production
4. infection of lymphocytes and macrophages
1. latent
2. chronic
3. oncogenic
4. slow
Chronic
Slow
Latent
oncogenic
Chronic infections are continuously detectable
1.
2.
3.
4.
immune status
genetic constitution
age
miscellaneous factors (ie hormonal and
nutritional factors)
8/30/2004
What type of microbes live at peace
with the human body?
What are the commensual microbe
population in our body called?
What type of microbe comprises the
majority of commensual organisms in
the natural flora?
*What are other types of commensual
organisms?
*Where do more fungi and protozoa
exist?
How often does the normal flora
change?
According to the prof how many
bacteria are in the mouth?
Can commensual microbes become
pathogenic?
Is there a way to tell a good bacteria
from a bad bacteria?
*What can cause a good bacteria to
become a bad bacteria?
*What is the measure of pathogenicity?
*What is any component of a
pathogenic microbe that contributes to
its pathogenicity.
*What is helping to find virulence
factors?
*What are the four determinants of
pathogenicity?
*What are the three methods of
transmission?
What type of process is transmission?
*What are specific bacterial
transmission forms?
*What type of change occurs to the
1.
2.
non-cultural methods
cultural methods
immunological methods
microscopic methods
detection of microbes by probing for their
genes
solid or liquid media are used for bacterial and
fungal growth
Cultured cells derived from animals and
humans are used for viral growth.
identify organisms
detect antibodies in a patients body fluid.
1.
2.
3.
4.
1.
2.
water
agar
growth-enriching constituents
blood
discrete colony formation
observation of colonial characteristics helpful
in identification of organisms
3. quantification as colony forming units.
Liquid
1. inspection of colonial characteristics
2. examination of microscopic morphology and
staining characteristics
3. Identification of growth conditions
1. sugar fermentation and assimilation profile.
2. enzyme profile
1. slide agglutination
2. latex agglutination
3. immunofluorescence
4. Enzyme linked immunosorbent assay.
A sensitive microbe is inhibited by the standard
therapeutic dose of a particular drug.
An intermediate microbe is inhibited by a higher
dose than that of the starndard therapeutic dose.
Disc diffusion test
microorganism to an antimicrobial
agent?
How should you take a pus sample?
*How should you take a mucous
sample?
**What are the 4 main types of
laboratory procedures for the diagnosis
of viral infections?
*What is intubation?
*What type of sample is the typical
urinalysis?
*What are 5 pieces of information the
2. shock
Concentration
High concentration, Low concentration
1.
2.
3.
4.
5.
1.
2.
Staph
fever
activation of complement
activation of macrophages
stimulation of B lymphocytes
increased antibody production
hypotension
disseminated intravascular coagulation
cocci in tampons.
patient info
o
o
o
o
True
possible diagnosis
current antimicrobial therapy
physician information
type of specimen
Special media
False: Special treatment must be given to the
specimen
False, samples differ. Temperature control may be
necessary.
o
microscopy
o
growth and biochemical characteristics
o
immunologic tests
o
bacteriophage typing
o
molecular methods
The type of possible pathogen.
Dark field microscopy
Stains
1. gram stain
2. acid fast stain
NOW!!!!
(look it up)
1. selective
2. differential
3. enrichment
4. characteristic
Selective
Enhances the growth of the bacteria that you want
while inhibiting the growth of other bacteria.
Saminella and bile salt.
Enhancement media
The nutrient that the bacteria needs to survive is
built into the media.
Characteristic media
Uria
Differential
A staining mechanism built into the media stains
each bacteria a different color.
Identification of a microbe.
Treatment planning
o dilution susceptibility
o dik-diffusion test (Kirby-bauer method)