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Tap the syringe to make sure all of the leaf disks are at the bottom of the
solution.
6. Hold the syringe tip upward and expel the air by depressing the plunger
carefully. Stop pushing on the plunger before any solution comes out the tip.
7. Seal the tip of the syringe with one finger and hold tightly. Pull back on the
plunger to create a partial vacuum inside the syringe. Hold this for 10 seconds.
8. While still holding the vacuum in the syringe, swirl the leaf disks in the solution.
The solution will fill in the air spaces in the leaf, causing the disks to sink.
9. Holding the syringe tip upward, simultaneously release the syringe plunger and
tip to release the vacuum.
10. If the leaf disks did not all sink inside the syringe barrel, repeat steps 79 until
all of the leaf disks sink to the bottom of the syringe barrel.
11. Place the syringe, plunger side down, on the work surface approximately 15
20 centimeters from the light source (adjust the distance accordingly so that the
solution does not absorb excess heat). The light source should be at least 60
Watts.
12. Begin timing the experiment as soon as the light source is turned on. Record
the number of disks that are floating at the top of the solution at the end of each
minute in your data table. After each time check, tap the side of the syringe to
make sure disks are not sticking to the container walls.
13. Continue timing and observing until all 5 disks in are floating.
14. Repeat steps 111 with a new set of 5 disks. Place the syringe upright on the
plunger and cover with a large bucket, bowl, or foil to prevent exposure to light.
15. Begin timing the experiment, recording the number of disks that are floating at
the end of each minute. Check the number of disks quickly to prevent extended
exposure to light.
16. Continue timing and observing for a total of 15 minutes. Record all
observations in the data table.
Data
Time
(Minutes)
Number of
Number of
Disks Floating
Disks Floating
(Light)
(Dark)
10
11
12
13
14
15
Conclusion:
I hypothesised that if I placed small leaf disks to a solution of baking exposed to light,
then they would float faster because the light increases the rate of photosynthesis. My data
showed that this was a correct hypothesis and that discs in the solution that was exposed to
light did float much quicker to the top. This was because the light expedited the process of
photosynthesis and caused the leaves to float. The lack of light and photosynthesis caused the
leaves to consume as much oxygen as it could from its surrounding and had a much harder
time consuming enough oxygen to float to the top.
1. What was the independent variable and dependent variable in this
experiment?
The independent variable was the amount of light the leaf disks were exposed to. The
dependent was the amount of time it took for them to float.
3. Did the results support the hypothesis in the experiment? Support your answer.
Yes because just like i hypothesized the leaf disks did float up faster when exposed to the
light.