New Biotechnology Volume 29S September 2012

Keywords: Antibacterial; Lactic acid bacteria; Escherichia coli;

Lactobacillus delbrueckii
http://dx.doi.org/10.1016/j.nbt.2012.08.352
Poster 3.2.18
Inuence of boron toxicity on global expression proles
in wheat (Triticum aestivum L.)
C. Kayihan 1, , M.T. Oz 1 , F. Eyidogan 2 , M. Yucel 1 , H.A. Oktem 1
1

Middle East Technical University, Department of Biology, 06800

Ankara, Turkey
2
Baskent University, Faculty of Education, 06810, Ankara, Turkey
Excess boron (B) is known to negatively affect plant growth.
However, the molecular mechanism of toxicity in plants is not satisfactory elucidated. In this study, global expression differences in
wheat (Triticum aestivum L. cv. Atay-85 and Bolal-2973) leaves and
roots under B toxicity were investigated using DNA microarrays.
Eight day old aseptically grown seedlings were subjected to 5 mM
H3 BO3 for 5 days. Total RNAs from leaf or root tissues were isolated
and checked by Bioanalyzer 2100. Expression proles were determined with Wheat GeneChip. Among 61127 transcripts each
represented with a probe set on the GeneChip fold change of at
least 2 and p value of at most 0.05 was considered as an indication
of signicant alteration in expression. Compared to unstressed
control (10 M H3 BO3 ) conditions, B toxicity resulted in significant regulation of more than 1000 transcripts in leaf and root
tissues of both cultivars. Furthermore, the numbers of genes significantly expressed in Atay-85 were higher than those in Bolal-2973.
Transcripts altered under B toxicity included genes coding for transcription factors, peroxidases, ribosomal proteins, and hydrolases
and functioning in biosynthesis of phenylpropanoids. Responsive
genes determined in this study will provide insight into successive studies on molecular mechanism of tolerance to B toxicity in
wheat.

spectrometry we monitored the treatment of diet-induced obese

mice with the amorfrutins and the diabetes drug rosiglitazone in
adipose tissue, liver and the heart. In contrast to microarray-based
gene expression analysis, we detected striking indication for the
unwanted molecular events of rosiglitazone leading to heart failure
and other known side effects. The methodology introduced here
can be used for systematic proteome-wide screenings of drug candidates and can be adapted further to human patient biopsies. In
summary, we showed that application of diet-derived compounds
may constitute a promising strategy to combat common metabolic
diseases.

Reference
1. Weidner C, et al. Proc Natl Acad Sci USA 2012;109(May (19)):725762.

http://dx.doi.org/10.1016/j.nbt.2012.08.354
Poster 3.2.20
Liposomes and liposomal nanoemulsions from sunower
S. Shulga , I. Glukh
Institute for Food Biotechnology and Genomics, National Academy of
Science of Ukraine 2, Osypovskogo str., 04123, Kyiv-123, Ukraine

Christopher Weidner, David Meierhofer, Sascha Sauer

Sunower lecithin gives manufactures an attractive alternative

to soy lecithin, without requiring allergen labeling. Sunower
lecithin is an ideal solution for producing chocolate, chewing gum,
sauces or instantized foods offering smoothness, ne texture and
good emulsifying properties and drug delivery systems. Use of the
sunower phosphatide concentrate as source material is one of
promising trends for the commercial use of phosphatidylcholine.
Liposomal technology is readily applied in medicine, cosmetology and pharmacology. The experience of encapsulation of
bioactive substances can be useful in food technology.
In the study liposomes were prepared from sunower lecithin
by some methods. The structure of liposomes and particle size
were analyzed and compared. The possibility of using empty
liposomes as transporting nanosystems was studied. Technology
parameters and conditions for their stability and long-term storage
were investigated as well.
Keywords: Lecithin; Sunower; Liposomes

Otto Warburg Laboratory, Max Planck Institute for Molecular Genetics,

Ihnestrae 63-73, 14195 Berlin, Germany

http://dx.doi.org/10.1016/j.nbt.2012.08.355

http://dx.doi.org/10.1016/j.nbt.2012.08.353
Poster 3.2.19
Amorfrutins are efcient natural antidiabetics

Worldwide increases in the incidence of metabolic diseases call for

alternative strategies for preventing and treating these disorders.
Compounds from edible natural resources provide a structurally
diverse resource to alleviate complex diseases by nutritional intervention. We discovered a family of natural antidiabetics, the
amorfrutins, isolated from two legumes, Glycyrrhiza foetida and
Amorpha fruticosa [1]. The amorfrutins selectively activate the
nuclear receptor PPAR, which results in gene expression and physiological proles markedly different from activation by known
synthetic PPAR ligands. In metabolic mouse models, amorfrutins strongly increased insulin sensitivity and improved other
physiological parameters. Using proteome-wide quantitative mass

Poster 3.2.21
The effect of heat processing on PCR detection of Genetically Modied (GM) soya in meat products
zge zgen Arun, Karlo Murato
glu, Funda Yilmaz
Istanbul University, Veterinary Faculty Food Hygiene, Technology
Department, Turkey
The plantation of GMOs are growing rapidly since they were rst
commercialized in 1996. Signicant ratio of globally planted soya
is GM. However the public concern over GMOs is growing as
rapidly. Thus most countries and nally Turkish Republic have
released regulations on labeling of GMOs. For this purpose develwww.elsevier.com/locate/nbt S127

New Biotechnology Volume 29S September 2012

oping sensitive methods for detection of GMOs is necessary. PCR is

the most common method used thus far. However processing factors (low pH, heat, etc.) effects the DNA quality and PCR detection.
Addition of soya to the meat products is common in many countries and most of them have low pH and heated. The aim of this
study was to evaluate combine effect of heat and pH on PCR detection of GM soya. Sausage mixtures containing various amounts of
GM soya were prepared, and process simulations were performed
representing common pH values and heating temperatures used
for meat products. The effect of heat was inspected by detection
of CaMV 35 S which is a novel sequence present in most GMOs
and lectin which is present in both GM and non GM soya. The
results proved that low amounts of GM soya can still be detected
in processed meat products although low pH and temperature has
an effect on detection limit.
Keywords: GMO; PCR; Soya; DNA
http://dx.doi.org/10.1016/j.nbt.2012.08.356
Poster 3.2.22
Heterologous expression of a barley boron transporter
gene in tobacco (Nicotiana tabaccum) and analysis of
transgenic plants
gan 3 , Meral Ycel 2 ,
Do
ga Gmsel 1, , M. Tufan Oz 2 , Fsun Eyido
Yelda zden C
iftci 1 , Hseyin Avni ktem 2
1

Gebze Institute of Technology, Department of Molecular Biology,

41400 Kocaeli, Turkey
2
Middle East Technical University, Department of Biological Sciences,
06800 Ankara, Turkey
3
Baskent University, Faculty of Education, 06810, Ankara, Turkey
High concentration of boron (B) in soil decreases agricultural yield
in arid and semi-arid regions throughout the world. A proposed
strategy to confer tolerance to B stresses in crops is stable transformation of target plants with genes encoding B tolerance proteins
such as efux/inux transporters. Therefore in this study, barley B
transporter gene HvBor1a was transferred to the model organism
tobacco via Agrobacterium-mediated transformation to improve
tolerance to B toxicity. Plant transformation vectors pIPKb004
containing HvBor1a and pEarleyGate100 containing HvBor1a-GFP
fusion were transferred to A. tumefaciens strain C58C1 using electroporation. Leaf disc transformation of tobacco (cv. Samsun and
Petit Havana) and indirect organogenesis were utilized to generate
putative transgenics constitutively expressing HvBor1a. The integration and expression of gene of interest was conrmed with
molecular analysis. Additionally, B toxicity bioassays were performed to examine the altered phenotype of transgenics and rst
generation of plants. In conclusion, results of this study suggested
that the HvBor1a gene might be of great value in engineering tolerance to B toxicity in crop plants.
http://dx.doi.org/10.1016/j.nbt.2012.08.357

S128

www.elsevier.com/locate/nbt

Poster 3.2.23
Continuous nisin production of Lactococcus lactis immobilized with surface displayed chitin binding domain on
chitin
Burcu Krdikanlio
glu , mer Simsek
University of Pamukkale, Department of Food Engineering, Denizli,
Turkey
In this study, nisin production of Lactococcus lactis strains expressing surface displayed chitin binding domain (ChBD) were analyzed
in a chitin containing continuous fermentation system. In this
respect, two ChBD-displayed L. lactis strains (PLAC2 and PLAC7),
previously constructed with fusing the chitin binding domain to
C-terminal part (344 or 800 amino acid) of the PrtP of L. lactis, were
used separately. These ChBD-displayed strains were immobilized
to chitin and subsequently included in a continuous fermentation
system with 0.10.9 dilution rates. Nisin production and biomass
concentration dramatically reduced for control strains at high
dilution rates where high nisin and biomass production was maintained at both ChBD-displayed L. lactis strains. Maximum nisin
production at control strains used in a fermentation system with
or without chitin was determined as 2120 IU ml1 where ChBDdisplayed strains with 344 aa or 800 aa C-terminal of prtP produced
4920 IU ml1 and 5520 IU ml1 nisin respectively. Besides, nisin
productivity of control strains with or without chitin existence was
measured as 812 IU ml1 h and 636 IU ml1 h respectively. However, this was measured as 2709 IU ml1 h with L. lactis PLAC2 and
4581 IU ml1 h with L. lactis PLAC7 meaning that nisin productivity of cells could be improved 7-fold higher. We concluded that
800 aa long C-terminal part of PrtP is more functional and yielded
higher productivity. Also surface displayed chitin binding domain
at nisin producer L. lactis could be used efciently in continuous
fermentation system.
Keywords: Lactococcus lactis; Nisin; Chitin binding domain;
Continuous fermentation
http://dx.doi.org/10.1016/j.nbt.2012.08.358
Poster 3.2.24
Production of monoclonal antibody against Staphylococcal Enterotoxin A (SEA)
Arzu Pnarbas 1, , Elif Yolac 1 , zlem Ertekin 2 , Fatma Ycel 2
1

Kocaeli University, Faculty of Medicine, Department of Medical Genetics and Molecular Biology, Umuttepe, Kocaeli, Turkey
2
TUBITAK, MAM, Institute for Genetic Engineering and Biotechnology,
Gebze, Kocaeli, Turkey
Staphylococcal Enterotoxins (SEA) cause syndromes like toxic
shock, arthritis, allergic reactions and autoimmune diseases beside
food poisoning. The mortality rate is low for staphylococcal food
poisoning but for children and elderly the mortality rate is in the
range of 0.034.4%. Staphylococcal poisonings causes economical
burden due to loss in the production and medical costs.
SEA is the most common toxic, heat and acid-resistant toxin
among all the SEAs. SEA causes serious contamination around the