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MOLECULE
Proteins
Lipids
Carbs
Nucleic acids
Elements
CHONS
CHOP
CHO
CHOPN
6.2
o
Photoorganoheterotroph
Chemolithoautotroph***
Chemolithoheterotroph
Preformed
organic C
CO2
Preformed
Organic C
Chemoorganoheterotroph Preformed
Organic C
Light
Organic
Inorganic
Inorganic
Inorganic
Inorganic
Organic
Organic
EXAMPLES
Cyanobactiera
Sulfur bacteria
Diatoms
Green non sulfur
bacteria
Nitrifying bacteria
Iron-oxidizing
bacteria
Methanogens
Sulfur oxidizing
bacteria
Sulfur oxidizing
bacteria
Nonphotosynthetic
microbes
Pathogenic
microbes
o
6.4 Nitrogen, Phosphorus, Sulfur
o 3 Main elements necessary for microbial growth
Sulfur, Phosphate, & Nitrogen
o Nitrogen
Synthesize amino acid
Can be obtained from either organic or inorganic sources (Nitrate)
Enzymes used: Glutamate dehydrogenase, glutamine synthesis, glutamate
synthase (Ch 11)
Processes: assimilatory nitrate reduction & Nitrogen fixation [N2 NH3]
(Ch 11)
o Sulfur
Nitrogen can be obtained from organic or inorganic sources
Synthesize: methionine & cysteine
o Phosphorus
Can only be obtained from inorganic sources, produced in periplasm
o
6.5 Growth Factors
o Definition= organic compounds that cannot be synthesized by an organism but are
essential for its growth
3 major types growth factors/Cofactors????:
1) amino acids
o Used for protein synthesis
B) Secondary Transporter
Energy from PE of ion gradient
Antiport= sodium move out, proton move
insubstance move in opposite direction
Symport= proton & lactose simultaneously
enter cellsubstance linked move in same
direction
3) Group translocation
o
o Passive Diffusion
High concentration Low concentration
O2, CO2, H2O move via this method
No metabolic energy required
o
CHAPTER 11
Sugar nucleotides
Pentose 5 phosphate
Fructose 6 phosphate
Fructose 6 phosphate
Amino sugars
Dihydroxyacetonephosphate
Dihydroxyacetonephosphate
Sedoheptulose-7phosphate
Erythros-4-phosphate
Fructose-6phosphate
3-Phosphoglycerate
(3CPHASE)
Serine family
(tryptophan, glycine,
cysteine)
Heptose in LPS
Erythros-4phosphate
Aromatic family
(tyrosine &
tryptophan) &
vitamins|cofactors
(ubiquinone)
Phosphoenolpyruvate
Phosphoenolpyruvate Serine family
(tryptophan, glycine,
cysteine)
Pyruvate
Pyruvate family
(leucine, alanine)
Acetyl Coenzyme A
Oxaloacetate
2-oxoglutarate
Succinyl coenzyme A
o
11.3 CO2 Fixation via Autotrophs Energetically expensive
o 1) Calvin cycle
Oxaloacetate
Acetyl
coenzyme A
Asparate
Family
Methionine
Glutamate
fam
-glutamate
-glutamine
heme
heme
Reverse of EMP
3ATP & 2NADPH/ 1 CO2 | total: 3x6=18ATP, 2x6=12NADPH, 6 carbons
Also called reductive pentose phosphate
Archaea dont use calvin cycle
EUKARYOTES= occur stroma
BACTERIA= occur carboxysomes
3 phases
1) carboxylation phase
2)reduction phase
3)Regeneration phase
CarboxylationReduction PhaseRegeneration Phase
Rubulose 1,5 biphosphate (RuBP)enzyme [split spontaneously to PGA] catalyze CO2 RuBP2 PGA-
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