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(a)
(i)
(ii)
(b)
(c)
(i)
max 2
max 3
(ii)
(iii)
(i)
(ii)
At low temperatures/9C;
Relatively little kinetic energy/molecules only moving slowly;
Fewer collisions with enzyme;
Slower rate of reaction/takes longer for lactose to be reduced/some
substrate goes through unchanged;
or
Enzyme concentration limiting;
Substrate in excess;
Saturation of active sites/all occupied;
Some substrate goes through unchanged;
(d)
(i)
(ii)
max 3
1
[16]
2.
(a)
(i)
Energy put in to get reaction started (Look for idea of getting started);
(ii)
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(b)
(c)
(d)
(i)
(ii)
(i)
(ii)
max 2
3
[12]
3.
(a)
(b)
Add (Benedicts) reagent (to urine sample) and heat / heat the mixture;
red/ brown/ orange/ green/ yellow;
max. 2
[4]
4.
(a)
Glycerol / glyceride;
(b)
(i)
(ii)
2
[5]
>5. (a)
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max. 2
(b)
(c)
(i)
(ii)
1
[7]
6.
(a)
(i)
(ii)
(iii)
(b)
(c)
(ii)
max 2
max 6
[15]
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7.
(a)
(b)
(c)
(i)
Glycerol / glyceride;
(ii)
(iii)
Phosphorus / P;
(i)
(ii)
8.
(i)
NH2 ;
(ii)
1
[2]
9.
(b)
(c)
(i)
(ii)
(iii)
C12 ;
H22O11 ;
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(d)
1
2
3
4
5
6
7
8
9
max 6
[15]
10.
(a)
(b)
C12;
H22O11;
(i)
(ii)
(c)
2
[6]
11.
(a)
(i)
(ii)
(b)
(c)
Keeps pH constant;
So proteins / enzymes in mitochondria not denatured / affected;
(d)
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(e)
6 max
[15]
12.
(a)
(i)
(ii)
(iii)
(b)
2 max
(i)
5;
(ii)
3
[8]
13.
(a)
(b)
(c)
(d)
(i)
Lysosome;
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(ii)
Enzymes;
Digests / breaks down / hydrolyses (other molecules);
Reject cholesterol.
2
[7]
14.
(a)
(b)
(i)
(ii)
(i)
(ii)
(c)
2 max
15.
(a)
(b)
(i)
4;
(ii)
Not made of identical units/ monomers/ made of fatty acids and glycerol;
(i)
A
B
(ii)
O(xygen);
C(arbon);
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(c)
(i)
(ii)
2
[9]
16.
(a)
(b)
(c)
(i)
(ii)
1
[9]
17.
(a)
(i)
31/31.2;
(ii)
(c)
(d)
(i)
6;
(ii)
11;
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(e)
18.
(a)
(i)
(c)
(i)
(ii)
(d)
Peptide bond;
19.
(a)
(i)
Hydrolysis;
(ii)
(iii)
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max 2
1
(b)
(c)
(i)
(ii)
(d)
1
2
3
4
5
6
7
max 6
[15]
20.
(a)
(b)
(i)
(ii)
(i)
To keep pH constant;
A change in pH will slow the rate of the reaction / denature the amylase /
optimum for reaction;
(ii)
Purple/lilac/mauve/violet;
Do not allow blue or pink.
(iii)
Protein present;
The enzyme/amylase is a protein;
Not used up in the reaction / still present at the end of the reaction;
max 2
[7]
21.
(a)
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(b)
(c)
(d)
(e)
max 2
max 2
Outer layer of skin cells are dead; Do not respire/Do not contain
mitochondria; Do not produce ATP/release energy; Cells do not
have required proteins/carriers;
max 3
max 6
[14]
22.
(a)
(b)
(i)
150;
(ii)
27;
100;
number of peptide bond hydrolysed = total number present / all peptide
bonds have been hydrolysed;
(c)
(d)
(e)
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3 max
(f)
23.
(a)
amino acid;
(b)
violet/purple/mauve/lilac;
(c)
3
[5]
24.
(a)
(b)
(i)
Amino acid;
(ii)
2
[4]
25.
(a)
(b)
(i)
(ii)
Active site;
Loses shape;
As enzyme is denatured/tertiary structure lost;
Substrate no longer fits/no enzyme-substrate complex formed;
Many different ways of forming bonds;
Some bonds reformed;
(Enzymes with these) will be active/regain shape;
max 3
2
[7]
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26.
(a)
(b)
(i)
NH2:
(ii)
(iii)
1
1
(i)
363/364or434/435;
(ii)
1
[5]
27.
(a)
H
(b)
(c)
(i)
OOC. R
OOC. R
OOC. R
H
2 marks if fully correct;1 mark if glycerol attached to 3 fatty acids
(ii)
Condensation / esterification.
(i)
(ii)
2
[7]
28.
(a)
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(b)
(i)
(ii)
Condensation;
(iii)
Must have box correct (allow HN / NH, but must have C=O correct)
H
H2N
O
C
OH
CH 2
SH
1
[5]
29.
3
[5]
30.
(a)
(i)
(ii)
(iii)
(b)
From 0.3 mol dm3 to 0.1 mol dm3 water moves into potato cells;
By osmosis;
So mass increases;
More water has entered potato cells 0.1 mol dm3 / converse;
0.35;
No mass change/no net osmosis/
volume of water in = volume of water out;
2
[10]
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31.
(a)
(i)
(ii)
(b)
(c)
(d)
2 max
2
[10]
32.
(a)
D;
(b)
A;
(c)
B;
(d)
E;
1
[4]
33.
(a)
(b)
Sciencemathsmaster.weebly.com
(i)
48
(ii)
description
increase up to 48 / optimum allow ECF from (i);
decrease above 48 / optimum allow ECF from (i);
explanation of increase
increased KE / move faster;
therefore more collisions / more enzyme-substrate complexes formed;
with active site;
(ii)
(c)
56-58
explanation of decrease
denaturation / 3D structure changed / tertiary structure changed;
detail e.g. breaking of hydrogen / sulphur bonds; (reject peptide bonds)
shape of active site changed;
substrate no longer fits;
6 max
[14]
34.
(a)
A protein;
B fat /oil / lipid / triglyceride;
C reducing sugar / named;
1
1
1
(b)
(c)
[6]
35.
(a)
OOCR
OOCR
OOCR
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(b)
(c)
3
[6]
36.
(a)
C12 ; H22O11 ;
(b)
(i)
(ii)
2
[6]
37.
(a)
(i)
condensation;
(b)
(i)
D;
(ii)
C;
(iii)
A;
(c)
2 max
[6]
38.
(a)
(i)
fructose;
hydrolysis;
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1
1
1
(c)
(i)
(ii)
2
[7]
39.
(a)
(i)
(b)
(c)
circle drawn around either of the Hs on NH2 group and circle drawn
around the OH;
(i)
(ii)
peptide;
3
[7]
40.
(a)
(b)
(c)
peptide;
(d)
{reject peptide)
[7]
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