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Abstract Nine dogs with allergic dermatitis were bathed twice weekly with a 0.01% uocinolone shampoo
for 6 months to evaluate the shampoo's eects on the adrenalpituitary axis, serum chemistries, complete
blood counts and cutaneous histology. A group of 5 normal control dogs were bathed twice weekly for 6
months with the shampoo vehicle to determine whether or not the stress of bathing would have any eects on
the monitored indices. Skin biopsies were evaluated at the beginning and end of the study, and serum
chemistries and serum cortisols postadrenocorticotrophic hormone administration were evaluated every 8
weeks. There was no eect of the drug over time on serum chemistries or cortisol. Individual dogs in the
treatment group had decreased epidermal and dermal thickness as well as decrease in size or number of
sebaceous glands after treatment. None of the dogs exhibited the combination of morphologic changes
characteristic of an atrophic dermatosis due to hyperadrenocorticism.
Keywords: uocinolone, cortisol, allergy, shampoo.
INTRODUCTION
Treatment protocol
Dogs in group 1 were bathed twice weekly (34 days
apart) for 6 months by their owners using the
following protocol: each dog was rst bathed with a
cleansing oatmeal-based shampoo (Florida Foam
shampoo, Hills Dermaceuticals, Orlando, FL) to
remove dirt and grease from the hair. The dog was
then thoroughly rinsed and then bathed with a 0.01%
uocinolone containing shampoo (FS shampoo, Hills
Dermaceuticals, Orlando, FL). The owners were
instructed to apply sucient shampoo to completely
cover the dog's coat, and enough to penetrate the
coat and contact the skin over the entire body. The
shampoo was massaged into the coat and remained in
3
177 DISC
4
K. M. Beale et al.
Time
Treatment group (n = 9)
mean pre (+ SD) mean post (+ SD)
Control group (n = 5)
mean pre (+ SD) mean post (+ SD)
Baseline
2 months
4 months
6 months
66.2
85.0
111.7
68.7
69.3
69.8
81.7
72.8
(22.9)
(61.0)
(52.1)
(32.6)
296.6
294.4
276.2
252.4
(91.6)
(99.3)
(106.5)
(63.5)
(14.6)
(45.8)
(41.1)
(27.0)
319.5
280.0
258.2
267.3
(87.2)
(88.0)
(53.0)
(70.6)
The treatment group was bathed twice weekly for 6 months in a 0.01% uocinolone shampoo. The
control group was bathed twice weekly in the vehicle.
Serum cortisol concentrations were evaluated in dogs before and 2 h after administration of 20 IU
ACTH gel IM. Normal cortisols for this laboratory are 13.8165.5 nmol L71 pre-ACTH and 165.5
469.0 post-ACTH administration.
# 2000 Blackwell Science Ltd, Veterinary Dermatology, 11, 37
177 DISC
Long-term use of a steroid shampoo on dogs
pruritus was suciently controlled with the shampoo
and ea control. Two dogs in the treatment group and
one dog in the control group (dog with dietary
hypersensitivity) required treatment with antibiotics
for a supercial pyoderma during the course of the
study. All three dogs had a history of previous
pyoderma. One dog in the treatment group developed
bacterial otitis externa which was treated with a
topical gentocin preparation (gentocin ophthalmic
solution, Schering Plough Animal Health, Kenilworth, NJ) containing no glucocorticoids. Dog 5
developed aspiration pneumonia on week 5 and
shampooing was discontinued for 4 weeks, then
resumed for an additional 4 months. The cause of
the pneumonia was not determined.
Dogs were of various sizes and haircoat types. The
treatment group included a Yorkshire terrier, two
Retriever mixes (long coats), two Beagles, two
Greyhounds, a terrier-mix and a Springer Spaniel.
The control group included a Husky, a Greyhound,
a Labrador Retriever, a Bassett Hound and a
German Shepherd.
There was no signicant dierence over time
between the treatment group and the control group
with respect to laboratory tests (Table 1). No
individual dogs exhibited an abnormal response to
ACTH stimulation suggestive of iatrogenic Cushing's
disease (low-normal baseline serum cortisol with little
or no response to exogenous ACTH administration).
Individual dogs in both the treatment and control
groups exhibited changes in the histology of the
tissues (Tables 2 & 3). Five of nine dogs in the
treatment group had a decrease in either the size or the
number of sebaceous glands post-treatment. Six of
nine dogs had decreased epidermal thickness posttreatment. When pre-treatment biopsies were comTable 3. Changes in sebaceous
gland size and number in biopsies
from dogs treated with a 0.01%
uocinolone shampoo and a control shampoo
dog
dog
dog
dog
dog
dog
dog
dog
dog
1.5
7.5
4
2.5
3.5
3.5
3.5
3.5
6
NC
3.5
NC
2
7
NC
NC
1.5
NC
1.5
3.5
11
4
3.5
2.5
3
3.5
3.5
NC
2
3.5
NC
NC
1.5
4
2
2.5
2
6.5
3
2
2.5
NC
NC
1.5
NC
4
2
4
2.5
2
4
NC
NC
NC
NC
NC
1
2
3
4
5
6
7
8
9
Control group
dog 11
dog 12
dog 13
dog 14
dog 15
Treatment group
dog
dog
dog
dog
dog
1
2
3
4
5
normal
normal
prominent
normal
normal
dog
dog
dog
dog
6
7
8
9
Control group
dog 11
dog 12
dog 13
dog 14
dog 15
decreased
normal
prominent
normal
NC
decrease in size and number
decrease in size
decrease in number
prominent
numerous
normal
normal
NC
NC
NC
NC
increase in size
and number
decrease in size
NC
NC
decrease in size
normal
normal
normal
normal
normal
NC
decrease in size
NC
NC
decrease in size
normal
prominent
prominent
normal
normal
NC
NC
decrease in size
NC
NC
normal
normal
normal
normal
normal
NC
NC
NC
NC
NC
Treatment group dogs were bathed twice weekly in a 0.01% uocinolone shampoo on a twice weekly
basis for 6 months. Control group dogs were bathed in the same manner using a vehicle-control
shampoo. Sebaceous glands were dened as normal when lobules containing fully dierentiated, highly
vacuolated cells were readily identied. Sebaceous glands were dened as prominent if multiple lobules
(4 3) were associated with each adnexal unit. Post-treatment samples were compared to pre-treatment
samples for either a decrease in size or number of sebaceous glands. NC = no change.
# 2000 Blackwell Science Ltd, Veterinary Dermatology, 11, 37
177 DISC
6
K. M. Beale et al.
REFERENCES
1. Glaze, M.B., Crawford, M.A., Nachreiner, R.F., Casey,
H.W., Nafe, L.A., Kearney, M.T. Ophthalmic corticosteroid therapy. Systemic eects in the dog. Journal of
the American Veterinary Medical Association 1988; 192:
735.
2. Zenoble, R.D., Kemppainen, R.J. Adrenocortical
suppression by topically applied corticosteroids in
healthy dogs. Journal of the American Veterinary
Medical Association 1987; 191: 6858.
3. Moriello, K.A., Fehrer-Sawyer, S.L., Meyer, D.J.,
Feder, B. Adrenocortical suppression associated with
topical otic administration of glucocorticoids in dogs.
Journal of the American Veterinary Medical Association
1988; 193: 32931.
4. Meyer, D.J., Moriello, K.A., Feder, B.M., FehrerSawyer, S.L., Maxwell, A.K. Eect of otic medications
containing glucocorticoids on liver function test results
in healthy dogs. Journal of the American Veterinary
Medical Association 1990; 196: 7434.
5. Roberts, S.M., Lavacxh, J.D., Macy, D.W., Severin,
G.A. Eect of ophthalmic prednisolone acetate on the
canine adrenal gland hepatic function. American Journal
of Veterinary Research 1984; 45: 17114.
6. Moriello, K.A., Halliwell, R.E.W., Oakes, M.
Determination of thyroxine, triiodothyronine and
cortisol changes during simultaneous adrenal and
thyroid function tests in healthy dogs. American
Journal of Veterinary Research 1987; 48: 45862.
7. Feldman, E.C., Nelson, R.W. Hyperadrenocorticism. In:
Canine and Feline Endocrinology and Reproduction, 2nd
edn. Philadelphia; W.B. Saunders Co, 1996: 187255.
Resume Neuf chiens presentant une dermatite allergique ont ete shampooines deux fois par semaine pendant
6 mois avec un shampooing contenant 0.01% de uocinolone an d'evaluer les eets du shampooing sur l'axe
hypophyso-adrenalien, sur les constantes biochimiques et hematologiques, et sur l'aspect histologique de la
peau. Un groupe de 5 chiens normaux a servi de groupe controle et a ete shampooine avec le vehicule pour
determiner si le stress du traitement pouvait inuencer les indices surveilles. Des biopsies cutanees ont ete
realisees au debut et a la n de l'etude. Les valeurs biochimiques et des mesures du cortisol, avant et apres
stimulation a l'ACTH, ont ete etudiees toutes les 8 semaines. Aucun eet du traitement n'a ete observe sur les
valeurs biochimiques ou sur celles du cortisol. Quelques chiens du groupe traite ont presente une diminution
# 2000 Blackwell Science Ltd, Veterinary Dermatology, 11, 37
177 DISC
Long-term use of a steroid shampoo on dogs
de l'epaisseur epidermique et dermique et une diminution du nombre ou de la taille des glandes sebacees apres
traitement. Aucun chien ne presentait de modications compatibles avec une dermatose atrophique due a une
hyperadrenocorticisme. [Beale, K. M., Kunkle, G. A., Ginn, P. et Keisling, K. (Securite d'utilisation d'un
shampooing a 0.01% de uocinolone chez les chiens allergiques.) Veterinary Dermatology 2000; 11: 37.]
Resumen Nueve perros con dermatitis alergica fueron banados semanalmente con un champu con un 0,01%
de uocinolona durante 6 meses para evaluar los efectos del champu sobre el eje adrenalpituitario, los
analisis bioqu micos sericos, los contajes hematicos y la histolog a cutanea. Un grupo de 5 perros control
normales fueron banados dos veces a la semana durante 6 meses con el veh culo del champu para determinar
si el estres del bano ten a algun efecto sobre los ndices monitorizados. Las biopsias cutanas fueron evaluadas
al principio y al nal del estudio, y la bioqu mica serica y los cortisoles sericos despues de la administracion de
hormona adrenocorticotropica fueron evaluados cada 8 semanas. No hubo efecto del farmaco a lo largo del
tiempo sobre las bioqu micas sericas o el cortisol. Los perros en el grupo de tratamiento ten an una
disminucion del grosor epidermico y dermico as como una disminucion en el tamano o numero de glandulas
sebaceas despues del tratamiento. Ninguno de los perros mostro la combinacion de cambios morfologicos
caracter sticos de una dermatosis atroca debida al hiperadrenocorticismo. [Beale, K. M., Kunkle, G. A.,
Ginn, P. y Keisling, K. (Seguridad de la administracion prolongada de un champu con un 0,01% de
uocinolona en perros alergicos.) Veterinary Dermatology 2000; 11: 37.]
Zusammenfassung Neun Hunde mit allergischer Dermatitis wurden fur sechs Monate zweimal wochentlich
mit einem 0.01%-igen Fluocinolonshampoo gebadet, um die Auswirkungen des Shampoos auf die
Nebennieren-Hypophysenachse, Serum- und Blutwerte und kutane Histopathologie zu bewerten. Eine
Gruppe mit funf normalen Hunden wurde fur 6 Monate zweimal wochentlich mit dem Konstituens gebadet,
um festzustellen, ob der Badestress irgendeinen Einuss auf die Bewertungsindices hatte. Hautbiopsien
wurden zu Beginn und am Ende der Studie, Serumwerte und Kortisolwerte nach Verabreichung von
adrenokortikotrophem Hormon alle 8 Wochen bewertet. Serum- und Kortisolwerte wurden durch das
Shampoo nicht beeinusst. Einzelne Hunde in der Behandlungsgruppe hatten am Ende der Behandlung
dunnere Epidermis und Dermis sowie verminderte Anzahl und Grosse der Talgdrusen. Keiner der Hunde
zeigte die fur eine durch Hyperadrenokortizismus hervorgerufene atrophische Dermatitis kennzeichnenden
morphologischen Veranderungen. [Beale, K. M., Kunkle, G. A., Ginn, P. und Keisling, K.
(Langzeitverwendung eines 0.01%-igen Fluocinolon Shampoos bei allergischen Hunden.) Veterinary
Dermatology 2000; 11: 37.]