ABX Pentra 60 - Service Manual

ABX HEMATOLOGIE
B.P.7290
Rue du Caduce
Parc Euromdecine
34184 MONTPELLIER Cedex 04
FRANCE
Tel.: (33) 04 67 14 15 16
Fax : (33) 04 67 14 15 17
ABX PENTRA 60
TECHNICAL MANUAL
P/N RAA 016 AA
REVISIONS
NDEX
A
P/N REVISION REVISION
SECTION DATE
Creation
all
19/07/99
CONTENTS
1. SPECIFICATIONS
2. HYDRAULIC & PNEUMATIC PRINCIPLES
3. ELECTRIC & ELECTRONIC PRINCIPLES
4. ANALYSIS CYCLE TECHNOLOGY
5. SOFTWARE VERSION
6. OUTPUT FORMAT
7. ALARMS & ERROR LIST
8. MAINTENANCE PROCEDURES
9. TRAINING SLIDES
10. BLOWUPS VIEWS
11. SPARE PART LIST
Technical Manual
RAA 016 A Ind A
1-Specifications
1.
Technical specifications ............................................................................................. 2

1.1.
1.2.
1.3.
1.4.
1.5.
1.6.
1.7.
2.
Physical specifications ................................................................................................ 4

2.1.
2.2.
2.3.
2.4.
2.5.
2.6.
2.7.
2.8.
2.9.
3.
Repeatability ................................................................................................................................. 6
Linearity .......................................................................................................................................... 6
Carryover ........................................................................................................................................ 7
Reagents specifications ............................................................................................. 8

4.1.
4.2.
4.3.
4.4.
4.5.
4.6.
4.7.
5.
Power requirements ..................................................................................................................... 4

Operating temperature and humidity ..................................................................................... 4
Dimensions and Weight ............................................................................................................... 4
Minimum specimen volume .......................................................................................................... 4
Dilutions ratios .............................................................................................................................. 4
HGB measurement ........................................................................................................................ 5
Counting aperture diameters ..................................................................................................... 5
Wastes ............................................................................................................................................ 5
Reagent consumption ................................................................................................................... 5
Summary of performance data ................................................................................. 6

3.1.
3.2.
3.3.
4.
Parameters ..................................................................................................................................... 2
1.1.1. CBC mode ............................................................................................................................. 2
1.1.2. CBC + 5DIFF Mode ........................................................................................................... 2
Throughput analyses .................................................................................................................... 3
Tube Identification ..................................................................................................................... 3
Reagents ......................................................................................................................................... 3
Keyboard & Display ...................................................................................................................... 3
Data processing ............................................................................................................................. 3
Measurements and computation ............................................................................................... 3
Diluent: ABX DILUENT .............................................................................................................. 8

Lyse: ABX ALPHALYSE ............................................................................................................... 8
Lyse: ABX BIOLYSE .................................................................................................................... 9
Detergent: ABX CLEANER ......................................................................................................... 9
ABX EOSINOFIX ........................................................................................................................ 9
ABX BASOLYSE II .................................................................................................................... 10
Waste handling precautions ..................................................................................................... 10
Limitations .................................................................................................................... 11
5.1.
5.2.
5.3.
Maintenance .................................................................................................................................. 11
Blood specimens ........................................................................................................................... 11
Known interfering substances .................................................................................................. 11
Technical Manual
RAA 016 A Ind A
2-Hydraulic & Pneumatic principles

1.
Pentra 60 Hydraulic .................................................................................................... 2

1.1.
1.2.
1.3.
2.
Transmission tube list ................................................................................................................. 2

Hydropneumatic connections ..................................................................................................... 4
Functions of valves ....................................................................................................................... 7
Pneumatic diagrams ..................................................................................................... 8

2.1.
2.2.
2.3.
2.4.
2.5.
2.6.
2.7.
Pentra 60 general pneumatic diagram ..................................................................................... 8

ABX Alphalyse circuit ................................................................................................................. 9
Eosinofix circuit ......................................................................................................................... 10
Basolyse II circuit ...................................................................................................................... 11
Cleaner circuit ............................................................................................................................. 12
Diluent circuit ............................................................................................................................. 13
Waste circuit ............................................................................................................................... 14
Technical Manual
RAA 016 A Ind A
3-Electric & Electronic principles

1.
Pentra 60 Synoptic ...................................................................................................... 2
2.
Pentra 60 Main Board ................................................................................................. 3

2.1.
2.2.
2.3.
2.4.
3.
board general view ............................................................................................................. 3

board Test Points ............................................................................................................... 5
board Potentiometers........................................................................................................ 7
Board configuration ........................................................................................................... 8
Pentra 60 Boards ......................................................................................................... 9

3.1.
3.2.
3.3.
3.4.
3.5.
4.
Main
Main
Main
Main
Optical preampli board XAA 423 B ......................................................................................... 9

LCD & Keyboard board XAA 426 B ........................................................................................ 10
Motor relay board XAA 427 B ................................................................................................. 11
Carriage board XAA 428 B ...................................................................................................... 12
LED board XAA 429 B............................................................................................................... 13
Connections.................................................................................................................. 14
4.1.
4.2.
4.3.
4.4.
4.5.
4.6.
4.7.
4.8.
4.9.
4.10.
Horizontal Carriage Motor XBA 391 A ................................................................................. 14

Upper Fan XBA 393 A ............................................................................................................... 15
IR sensor XBA 394 AS ............................................................................................................. 16
IR sensor XBA 395 AS ............................................................................................................. 17
IR sensor XBA 396 AS ............................................................................................................. 18
IR sensor XBA 397 AS ............................................................................................................. 19
RBC / WBC coaxe XBA 398 A ................................................................................................. 20
LMNE flowcell coaxe XBA 399 AS ........................................................................................ 21
Barcode reader cable XBA 402 AS ....................................................................................... 22
Diluent level sensor XDA 605 AS .......................................................................................... 23
Technical Manual
RAA 016 A Ind A
4-Analysis Cycle Technology

1.
Analysis cycle description ......................................................................................... 2
2.
Measuring principles ................................................................................................... 4

2.1.
2.2.
2.3.
2.4.
2.5.
2.6.
2.7.
2.8.
2.9.
2.10.
Multi Distribution Sampling System (MDSS) ....................................................................... 4

RBC / PLT detection principles ................................................................................................. 5
Hgb measurement ......................................................................................................................... 6
Hct measurement ......................................................................................................................... 6
RDW Calculation ........................................................................................................................... 6
MCV, MCH, MCHC Calculation ................................................................................................... 7
MPV Measurement ........................................................................................................................ 7
Thrombocrit calculation .............................................................................................................. 7
PDW calculation ............................................................................................................................ 8
WBC count and differentiation ................................................................................................. 8
2.10.1.General principles ............................................................................................................. 8
2.10.2.BASO / WBC Count ......................................................................................................... 8
2.10.3.LMNE matrix ..................................................................................................................... 9
2.11. Dilutions summary ...................................................................................................................... 12
Technical Manual
RAA 016 A Ind A
5-Software Versions
1. Software Overview ........................................................................................................... 2
1.1. User Menu Overview ........................................................................................................................ 2
1.2. Technician Menu Overview ............................................................................................................. 3
2. Software Release ............................................................................................................. 4

2.1. Software Release Table ................................................................................................................. 4
2.2. Software Modifications ................................................................................................................. 4
Technical Manual
RAA 016 A Ind A
6-Output Format
1.
Argos format principles ............................................................................................. 2

1.1.
1.2.
1.3.
1.4.
2.
ABX format principles ................................................................................................ 5

2.1.
2.2.
2.3.
2.4.
2.5.
2.6.
2.7.
2.8.
3.
Introduction ................................................................................................................................... 2
Results characteristics ............................................................................................................... 2
1.2.1. Key ........................................................................................................................................ 2
1.2.2. Result format ..................................................................................................................... 3
Patient file characteristics ....................................................................................................... 4
1.3.1. Key ........................................................................................................................................ 4
1.3.2. Patient file format ............................................................................................................ 4
End of communication .................................................................................................................. 4
1.4.1. Key ........................................................................................................................................ 4
Message structure ....................................................................................................................... 6

Details about the structure ....................................................................................................... 6
Identifier list and their formats ............................................................................................. 7
2.3.1. Hematology numerical parameters ............................................................................... 7
2.3.2. Identifier list ..................................................................................................................... 8
Pathology ......................................................................................................................................... 9
2.4.1. Flags associated with parameters ................................................................................ 9
2.4.2. Pathological messages .................................................................................................... 10
Histograms and matrix.............................................................................................................. 13
2.5.1. Format description ......................................................................................................... 13
2.5.2. Format description of the threshold transmission ................................................ 14
Patient result identification .................................................................................................... 18
2.6.1. Format description ......................................................................................................... 18
2.6.2. Identifier list ................................................................................................................... 18
2.6.3. Analysis type ($80) ........................................................................................................ 19
Packet type .................................................................................................................................. 19
2.7.1. Data exported by the analyzer ................................................................................... 19
2.7.2. Data interpreted by the analyzer ............................................................................... 20
Other identifiers ........................................................................................................................ 20
Pin assignments .......................................................................................................... 21
Technical Manual
RAA 016 A Ind A
7-Alarms & Error List

1.
Error messages ............................................................................................................ 2

1.1.
1.2.
1.3.
1.4.
1.5.
1.6.
Printer ............................................................................................................................................. 2
Transmission .................................................................................................................................. 2
Calibration ...................................................................................................................................... 2
Technical Operations ................................................................................................................... 3
Reagents ......................................................................................................................................... 3
Temperature .................................................................................................................................. 3
Technical Manual
RAA 016 A Ind A
8-Maintenance Procedures
1.
Maintenance .................................................................................................................. 2
1.1.
1.2.
1.3.
1.4.
2.
Maintenance kits .......................................................................................................... 4

2.1.
2.2.
2.3.
2.4.
2.5.
3.
Introduction ................................................................................................................................... 2
Daily customer maintenance ...................................................................................................... 2
Weekly customer maintenance ................................................................................................. 2
Maintenance table ........................................................................................................................ 3
6 month Maintenance kit ............................................................................................................ 4

Yearly Maintenance kit ............................................................................................................... 5
Pistons Maintenance kit .............................................................................................................. 6
Fitting Kit ....................................................................................................................................... 7
Screws Kit ...................................................................................................................................... 8
Procedures ................................................................................................................... 10
3.1.
3.2.
Procedure chart tables .............................................................................................................. 11

Required tools and products .................................................................................................... 12
Technical Manual
9-Training slides
RAA 016 A Ind A
Technical Manual
RAA 016 A Ind A
10-Blowups views
1.
Blowups views ................................................................................................................. 1

1.1.
1.2.
1.3.
1.4.
1.5.
1.6.
1.7.
1.8.
1.9.
1.10.
1.11.
1.12.
1.13.
1.14.
1.15.
1.16.
1.17.
1.18.
1.19.
1.20.
1.21.
1.22.
1.23.
1.24.
1.25.
1.26.
1.27.
1.28.
1.29.
1.30.
1.31.
1.32.
1.33.
1.34.
1.35.
1.36.
1.37.
1.38.
1.39.
1.40.
1.41.
1.42.
1.43.
1.44.
1.45.
11 Valves assy ................................................................................................................................. 1

5 Valves assy (12-16) ................................................................................................................... 2
2 Valves assy .................................................................................................................................. 3
7 Valves assy .................................................................................................................................. 4
5 Valves assy (27-31) ................................................................................................................... 5
Optical bench syringe assy ........................................................................................................ 6
Reagents syringe assy ................................................................................................................. 7
Sampling syringe assy .................................................................................................................. 8
Syringe motorization carriage assy ......................................................................................... 9
Syringe motor assy .................................................................................................................... 10
Syringe motor body assy ........................................................................................................... 11
Sampling motor assy .................................................................................................................. 12
Optical syringe motor assy ...................................................................................................... 13
Reagents motor assy .................................................................................................................. 14
Sampling syringe assy ................................................................................................................ 15
Vacuum syringe piston assy ...................................................................................................... 16
Vacuum syringe assy .................................................................................................................. 17
Short vacuum syringe assy ....................................................................................................... 18
Long vacuum syringe .................................................................................................................. 19
Long vacuum syringe assy ......................................................................................................... 20
Short vacuum syringe assy ....................................................................................................... 21
Needle carriage motor assy ..................................................................................................... 22
Horizontal sampling carriage notched belt .......................................................................... 23
Notched belt ............................................................................................................................... 24
Needle carriage assy ................................................................................................................. 25
Sampling horizontal carriage ................................................................................................... 26
Sampling horizontal carriage assy .......................................................................................... 27
Sampling carriage assy .............................................................................................................. 28
Carriage vertical belt ................................................................................................................ 29
Carriage rinsing block assy ....................................................................................................... 30
Home carriage cell ..................................................................................................................... 31
Needle assy .................................................................................................................................. 32
Carriage motor assy ................................................................................................................... 33
Sampling needle assy ................................................................................................................. 34
Reagents heating coils assy ..................................................................................................... 35
Thermostated room assy .......................................................................................................... 36
Thermostated room plate assy ............................................................................................... 37
Thermostated room fan assy ................................................................................................... 38
Frame assy ................................................................................................................................... 39
Diluent chamber assy ................................................................................................................ 40
Diluent chamber .......................................................................................................................... 41
RETIC flowcell assy ................................................................................................................... 42
Optical bench assy ..................................................................................................................... 43
Optical bench lamp ..................................................................................................................... 44
Preamplifier support .................................................................................................................. 45
Technical Manual
RAA 016 A Ind A
11-Spare part list

1.
Spare part list ...............................................................................................................1
Technical Manual
RAA 016 A Ind A
ABX DIAGNOSTICS
ABX Diagnostics
B.P. 7290
Rue du Caduce
Parc Euromdecine
FRANCE
Tel.: (33) 4 67 14 15 16
Fax: (33) 4 67 14 15 17
ABX Diagnostics Training & Documentation Center

ABX Diagnostics
International Training & Documentation Center
B.P. 7290
Rue du Caduce
Parc Euromdecine
FRANCE
Standard: (33) 4 67 14 15 16
Direct line: (33) 4 67 14 15 36
Fax: (33) 4 67 14 15 17
NOTE
If you have some modifications, requests or adds to do to this manual,

please use the following document and send it to ABX Diagnostics Training
and Documentation Center
ABX Diagnostics Technical Informations

ABX Diagnostics
Technical Informations
B.P. 7290
Rue du Caduce
Parc Euromdecine
FRANCE
Tel.: (33) 4 67 14 15 16
Fax: (33) 4 67 14 15 17
REQUEST FORM FOR MODIFICATIONS OR

CORRECTIONS IN A DOCUMENT
To be filled in by the person who requested the modification
Requested by :
Date :
Instrument :
Document :
Request description :
To be filled in by the documentation department
Registered on :
Number :
Action performed :
Turnaround time :
Requested person informed on :

Modification achieved on :
Availability :
FORM-0101
1. SPECIFICATIONS
Content
1.
Technical specifications ....................................................... 2

1.1.
1.2.
1.3.
1.4.
1.5.
1.6.
1.7.
2.
Physical specifications .......................................................... 4

2.1.
2.2.
2.3.
2.4.
2.5.
2.6.
2.7.
2.8.
2.9.
3.
Repeatability .................................................................................... 6
Linearity ............................................................................................. 6
Carryover .......................................................................................... 7
Reagents specifications ........................................................ 8

4.1.
4.2.
4.3.
4.4.
4.5.
4.6.
4.7.
5.
Power requirements ....................................................................... 4

Operating temperature and humidity ........................................ 4
Dimensions and Weight .................................................................. 4
Minimum specimen volume ............................................................ 4
Dilutions ratios ................................................................................ 4
HGB measurement .......................................................................... 5
Counting aperture diameters ...................................................... 5
Wastes ............................................................................................... 5
Reagent consumption ..................................................................... 5
Summary of performance data ........................................... 6

3.1.
3.2.
3.3.
4.
Parameters ....................................................................................... 2
1.1.1. CBC mode ............................................................................... 2
1.1.2. CBC + 5DIFF Mode .............................................................. 2
Throughput analyses ...................................................................... 3
Tube Identification ....................................................................... 3
Reagents ............................................................................................ 3
Keyboard & Display ......................................................................... 3
Data processing ............................................................................... 3
Measurements and computation ................................................. 3
Diluent: ABX DILUENT ................................................................. 8

Lyse: ABX ALPHALYSE ................................................................. 8
Lyse: ABX BIOLYSE ...................................................................... 9
Detergent: ABX CLEANER ........................................................... 9
ABX EOSINOFIX ........................................................................... 9
ABX BASOLYSE II ...................................................................... 10
Waste handling precautions ....................................................... 10
Limitations............................................................................... 11
5.1.
5.2.
5.3.
Maintenance ................................................................................... 11
Blood specimens ............................................................................ 11
Known interfering substances ................................................... 11
1. Specifications
1. TECHNICAL
RAA 016 A Ind A
SPECIFICATIONS
The ABX PENTRA 60 is a fully automated (Microprocessor controlled)
hematology analyzer used for the in vitro diagnostic testing of whole
blood specimens.
The ABX PENTRA 60 is able to operate either in CBC mode (12
parameters) or in CBC + 5DIFF mode (26 parameters).
1.1. Parameters
1.1.1.
CBC mode
WHITE BLOOD CELLS
RED BLOOD CELLS
HGB
MCV
MCH
HCT
MCHC
RDW
1.1.2.
PLATELETS
PDW
MPV
PCT
CBC + 5DIFF Mode

WHITE BLOOD CELLS
Lymphocytes % and #
Monocytes % and #
Neutrophils % and #
Eosinophils % and #
Basophils % and #
LIC % and #
ALY % and #
RED BLOOD CELLS
HGB
MCV
MCH
HCT
MCHC
RDW
page 2/15
PLATELETS
PDW
MPV
PCT
1. Specifications
RAA 016 A Ind A
1.2. Throughput analyses

CBC Mode:
60/h
CBC + 5DIFF Mode:
60/h
1.3. Tube Identification

- By means of the front panel keyboard
- By means of barcode labels
1.4. Reagents
5 reagents, 4 Integrated
- ABX DILUENT (20 litres)

- ABX CLEANER (1 litre)
- ABX EOSINOFIX (1litre)
- ABX BASOLYSE II (1 litre)
- ABX ALPHALYSE or BIOLYSE (0.4 litre)
1.5. Keyboard & Display

- ABX specific keyboard, silicone made
- LCD, 128 x 240 pixels, LED Backlighted.
1.6. Data processing

- 68331 type microprocessor
- RS232C output
1.7. Measurements and computation

- Impedance change for WBC, PLT, RBC, BASO
- Spectrophotometry for HGB
- Impedance change and light scattering for LYM, MON, NEU, EOS,
ALY and LIC.
- Computation from stored data that was directly measured for HCT,
MCV, MCH, MCHC, RDW, MPV, PCT, PDW.
page 3/15
1. Specifications
2. PHYSICAL
RAA 016 A Ind A
SPECIFICATIONS
2.1. Power requirements

- Power supply:
* from 100Vac to 240Vac
* 50 Hz to 60 Hz
- Power Consumption:
* Maximum : 200 VA
2.2. Operating temperature and humidity

* 16 - 34C (61 - 93F) room temperature
* Maximum relative humidity 80% for temperatures
up to 31C (88F) decreasing linearly to 50%
relative humidity at 40C (104F).
2.3. Dimensions and Weight

- Dimensions:
* Height: approximately 516 mm (20.3 inches)
* Width: approximately 444 mm (17.5 inches)
* Depth: approximately 481 mm (19 inches)
- Weight:
* approximately 35Kgs (77lbs).
2.4. Minimum specimen volume

* CBC Mode:
30l
* CBC + 5DIFF Mode:
53l
2.5. Dilutions ratios
page 4/15
* WBC/BASO:
1/200
* LMNE:
1/80
* RBC/PLT:
1/10 000
* HGB:
1/250
1. Specifications
RAA 016 A Ind A
2.6. HGB measurement

* HGB chamber, LED 555 nm.
* Modified Drabkin method (cyanmethemoglobine)
* Light source: Electroluminescent diode
* Wavelength: 550nm + 10nm
2.7. Counting aperture diameters

* WBC/BASO = 80 m
* LMNE = 60 m
* RBC/PLT = 50 m
2.8. Wastes
Waste handled according to local/national regulations.
2.9. Reagent consumption
* for one background count only (maxi = 3)
page 5/15
1. Specifications
RAA 016 A Ind A
3. SUMMARY
OF PERFORMANCE DATA
3.1. Repeatability
Based on 20 consecutive samplings from one fresh whole blood sample
without alarm.
PARAMETERS
% CV
TE S T LE V E L
- WBC :
<2%
at 10.109 /l
- RBC :
< 2%
at 5.1012 /l
- HGB :
< 1%
at 15 g/dl
- HCT :
< 2%
at 45 %
- MCV :
< 1%
at 90 fl
- PLT :
< 5%
at 300.109 /l
3.2. Linearity
Linearity was tested using a commercially available low range and full
range linearity test kits. The kits were analyzed and data was computed
according to the manufacturer's instructions. Each kit included six
levels and one level was used as the reference value. Each level was
run four times. The results of this study were as follows:
PAR AME T E R S
L I N E AR I T Y
R AN G E
L IM IT S
W B C (x1 0 3 c e lls / L )
0 .5 to 8 0
+/- 0 .2 o r +/- 3 % (whi c he ve r i s g re a te r)
RB C (x1 0 6 c e lls / L )
0 .2 to 7 .5
+/- 0 ,0 5 o r +/- 2 % (whi c he ve r i s g re a te r)
P LT (x1 0 3 c e lls / L )
1 0 to 1 0 0 0
+/- 1 0 o r +/- 6 % (whi c he ve r i s g re a te r)
2 .5 to 2 3
+/- 0 ,3 o r +/- 2 % (whi c he ve r i s g re a te r)
11 .6 to 5 5
+/- 2 o r +/- 3 % (whi c he ve r i s g re a te r)
5 6 to 6 2 .4
+/- 5 o r +/- 5 % (whi c he ve r i s g re a te r)
HG B ( g / d L )
HC T ( % )
page 6/15
1. Specifications
RAA 016 A Ind A
3.3. Carryover
Carry-over was tested by analyzing samples with high concentrations
of WBC's, RBC's, HGB and PLT's. Each sample was run in triplicate,
followed by three background cycles. The % carryover is calculated
using the following formula:
Background1 - Background3
Carryover = ------------------------------------------ X 100
Sample3 - Background3
WB C
RBC
H GB
P LT
6 3 .0
7 .5 8
2 3 .4
988
% C a r r y- o ve r ( a c tua l)
0 .3
0 .0 0
0 .0
0 .0
% C a r r y- o ve r ( c la i m )
< 0 .5 %
< 0 .5 %
< 0 .5 %
< 0 .5 %
L e ve l
page 7/15
1. Specifications
4. REAGENTS
RAA 016 A Ind A
SPECIFICATIONS
In order for the instrument to operate correctly, high-quality reagents
must be used. ABX Diagnostics provides all the necessary reagents.
NOTE:
- All these reagents have been registered by the "Agence du Mdicament" according to the
statuts relative to laboratory reagents used for biological analyses.
- These reagents are used for in vitro diagnostics.
- The use and results for these reagents are described into the section 2 and 3 of this manual.
- All these reagents are manufactured by ABX Diagnostics - Rue du Caduce - Parc Euromdecine
34184 MONTPELLIER CEDEX - FRANCE - Tel : (33) 4 67 14 15 16 - Fax : (33) 4 67 14 15 17.
4.1. Diluent: ABX DILUENT

Function: This diluent is necessary for the process involved in
counting (and differentiating) the blood cells.
Composition: Stabilized saline solution which contains an organic
buffer, an antiseptic and Sodium Azide < 0.1%.
Description: Limpid and odorless aqueous solution.
Physico-chemical properties: Boiling point : About 100C, pH :
neutral.
Handling Precautions: Avoid skin and eye contact. Use laboratory
gloves when handling the reagents. If a large quantity of reagent is
ingested a mucous irritation can result.
Emergency First aid: If the eyes or skin come into contact with the
reagent, flush / rinse abundantly with water. If a large quantity is
ingested, drink water immediately, and induce vomiting.
stored at 18C to 25C away from the light
4.2. Lyse: ABX ALPHALYSE

Function: These reagents are used to lyse blood cells and determine
hemoglobin concentration. May be dangerous.
Composition:The reagent contains potassium cyanide at 0.03%, a
quarternary ammonium salt and a saline phosphate buffer containing
sodium azide < 0.1%.
Description: Aqueous solution, limpid.
Physico-chemical properties: Boiling point: approximately 100C.
pH: basic, smells of cyanide.
page 8/15
1. Specifications
RAA 016 A Ind A

Handling Precautions: Avoid contact with eyes, skin and clothing.
Wear laboratory gloves when handling the product. The product
may be harmful if ingested. The product can be absorbed through
an open wound, or inhalation.
reagent, flush with water. If the reagent is inhaled, breathe fresh air
immediately. If a large quantity is ingested, drink water immediately,
and induce vomiting. Call local anti-poison center, or contact doctor.
stored at 18C to 25C away from the light
4.3. Lyse: ABX BIOLYSE

Function: These reagents are used to lyse blood cells and determine
hemoglobin concentration.
Composition:quaternary ammonium chloride
Description: Colorless, Odorless.
Physico-chemical properties: pH: 6.65
Handling Precautions: Avoid contact with eyes. The product may
be harmful if ingested.
reagent, flush with water. If the product is ingested, call immediately
a doctor.
storage conditions: no particular condition
4.4. Detergent: ABX CLEANER

Function: Washing agent. May be harmful.
Composition: Enzymatic solution with proteolytic action.
Description: Transparent liquid
Physico-chemical properties: Boiling point: around 100C.
pH = 9.6.
Handling Precautions: Avoid contact with eyes, skin and clothing
Emergency First Aid: In case the product comes into contact with
the eyes, rinse with water. If the product is ingested, call immediately
a doctor.
stored at 18C to 25C
4.5. ABX EOSINOFIX

Function: This reagent lyses RBCs, fixes leukocytes and gives a
specific coloration to eosinophils.
Composition: Alcoholic solution containing propylene-glycol, a formic
dye, buffers, alkaline salts, wetting agents and an aldehyde
preservative.
page 9/15
1. Specifications
RAA 016 A Ind A

Description: Deep blue aqueous solution, smells of alcohol.
Physico-chemical properties: pH: 6,9.
Handling precautions: Avoid contact with eyes, skin and clothing.
may be harmful if ingested or inhalted. Keep the bottle closed when
not in use.
Emergency First Aid: If the eyes or skin come into contact with the
reagent, flush with water. If the reagent is inhaled or ingested, call
local anti-poison center or contact doctor.
Storage conditions: Room temperature between 18 and 25C.
4.6. ABX BASOLYSE II

Function: This reagent lyses RBCs for the leucocytes count and
differentiation of the polynuclear basophils.
Composition: Acidic solution containing a lytic agent.
Description: Colorless aqueous solution.
Physico-chemical properties: pH: 2.4.
Handling precautions: Avoid contact with eyes, skin and clothing.
may be harmful if ingested or inhalated. Keep the bottle closed
when not in use.
Emergency First Aid: If the eyes or skin come into contact with the
reagent, flush with water. If the reagent is inhaled, breath fresh air
immediately. If a large quantity is ingested, drink water immediately.
Do not induce vomiting. Call local anti-poison center or contact
doctor.
Storage conditions: Room temperature between 18 and 25C.
4.7. Waste handling precautions

If your local or national organizations require, waste liquids can be
neutralized before being discarded.
Waste liquids of the ABX PENTRA 60 can be neutralized using the
following procedure:
- For 20L of waste liquids, add 50mL of Sodium Hydroxide solution
200g/L, then 250mL of bleach solution 12Cl daily prepared from
the commercialized solution (48Cl).
Expired reagents
- Cyanides from the lysing reagent : for 1L of lysing reagent, add
50mL of Sodium Hydroxide 200g/L, then 100mL of Ammonium
Persulfate solution 500g/L daily prepared, or add 50mL of Sodium
Hydroxide 200g/L, then add 500mL of Sodium Hypochloride solution 30%.
page 10/15
1. Specifications
RAA 016 A Ind A
5. LIMITATIONS
5.1. Maintenance
In section 8, specific maintenance procedures are listed. The maintenance procedures identified are mandatory for the proper use and
operation of the ABX PENTRA 60.
FAILURE TO EXECUTE ANY OF THESE RECOMMENDED PROCEDURES MAY RESULT IN POOR RELIABILITY OF THE SYSTEM.
5.2. Blood specimens

Verification of any abnormal test result (including flagged results or
results outside of the normal range) should be performed using
reference methods or other standard laboratory procedures for conclusive verification of the results.The sections below list known limitations of automated blood cell counters which use the principles of
impedance and light absorbance as principles of measurement.
5.3. Known interfering substances

WBC : White Blood Cells (Leukocytes)
WBC results that exceed the linearity limits of the system will require
dilution of the blood sample (Leukemia sample followed by a
leukopenia). Re-assaying the diluted sample will help to obtain the
correct assay value.
Unlysed Red Cells - In some rare instances, the erythrocytes in the
blood sample may not completely lyse. These non-lysed red blood
cells may be detected on the WBC histogram with an L1 alarm or as
an elevated baseline on the side (leading edge) of the lymphocytes
population. Non-lysed erythrocytes will cause a falsely elevated WBC
count.
Multiple myeloma - The precipitation of proteins in multiple myeloma
patients may give elevated WBC counts.
Leukemia - A very low WBC count may result in this disease state
because of possible increased fragility of the leukocytes leading to
some destruction of these cells during counting. These white cell fragments will also interfere with the white cell differential parameters.
Chemotherapy - Cytotoxic and immunosuppressive drugs may
increase the fragility of the leukocytes which may cause low WBC
counts.
page 11/15
1. Specifications
RAA 016 A Ind A

Cryoglobulins - Increased levels of cryoglobulin that may be associated
with myeloma, carcinoma, leukemia, macroglobulinemia,
lymphoproliferative disorders, metastic tumors, autoimmune disorders,
infections, aneurism, pregnancy, thromboembolic phenomena,
diabetes, etc, which can elevate the WBC, RBC or PLT counts and
the HGB concentration. The specimen must be warmed up to 37C in
a bain marie during 30 minutes and reanalyzed immediately (analyzer
or manual method).
RBC Red Blood Cells (Erythrocytes):
The red blood cell dilution contains all the formed elements in the
blood: erythrocytes, leukocytes and platelets. During the counting of
the erythrocytes (red blood cells), platelets are not counted as their
size falls below the minimum threshold.
Agglutinated erythrocytes - May cause a falsely low RBC count.
Blood samples containing the agglutinated red blood cells may be
suspected by elevated MCH and MCHC values and shown by
examination of the stained blood film.
Cold agglutinins - IgM immunoglobulins which are elevated in cold
agglutinin disease may lower RBC and PLT counts and increase MCV.
HGB (Hemoglobin):
Turbidity of the blood sample - Any number of physiologic and/or
therapeutic factors may produce falsely elevated HGB results. To obtain
accurate hemoglobin results when increased turbidity of the blood
sample occurs, determine the cause of the turbidity and follow the
appropriate method below:
1. Elevated WBC: An extremely elevated WBC will cause excessive
light scatter. In these cases use reference (manual) methods.The
diluted sample should be centrifuged, and the supernatant fluid
measured with a spectrophotometer.
2. Elevated lipids: Elevated lipids in the blood sample will give the
plasma a milky appearance. This condition can occur with
hyperlipidemia, hyperproteinemia (as in gammapathies) and
hyperbilirubinemia. Accurate hemoglobin determinations can be
achieved by using reference (manual) methods and a plasma blank.
page 12/15
1. Specifications
RAA 016 A Ind A

3. Increased turbidity may also be seen in cases where the red
blood cells are resistant to lysing. This condition will cause a falsely
elevated Hgb result, but may be detected by observing the abnormal
MCH, MCHC values, and the increased baseline on the leading edge
of the WBC histogram. Erroneous hemoglobin results will cause the
results of the MCH and MCHC to be erroneous as well.
4. Fetal bloods - The mixing of fetal and maternal bloods may produce
a falsely elevated HGB value.
HCT (Hematocrit)
Red blood cells agglutination - May produce erroneous HCT and
MCV values. Red blood
cells agglutination may be detected by observing abnormal MCH and
MCHC values, as well as by examination of the stained blood film In
such cases, manual methods may be required to obtain an accurate
HCT value.
MCV (Mean Corpuscular Volume)
Red blood cell agglutination - May produce an erroneous MCV
value. Red blood cell agglutination may be detected by observing
abnormal MCH and MCHC values, as well as by examination of the
stained blood film. In such cases, manual methods may be required
to obtain an accurate MCV value.
Excessive numbers of large platelets and/or the presence of an
excessively high WBC count may interfere with the accurate
determination of the MCV value. In such cases, careful examination
of the stained blood film may reveal the error.
MCH (Mean Corpuscular Hemoglobin)
The MCH is determined according to HGB value and the RBC count.
The limitations listed for the HGB and RBC will have an effect on the
MCH and may cause erroneous values.
MCHC (Mean Corpuscular Hemoglobin Concentration)
The MCHC is determined according to the HGB and HCT values. The
limitations listed for the HGB and HCT will have an effect on the MCHC
and may cause erroneous values.
RDW (Red blood cell Distribution Width)
The red blood cell distribution width is determined according to the
RBC count.
page 13/15
1. Specifications
RAA 016 A Ind A

Agglutinated erythrocytes - May cause a falsely low RBC count
and erroneous RDWs. Blood samples containing the agglutinated RBC
may be detected by observing abnormal MCH and MCHC values, as
well as by examination of the stained blood film.
Nutritional deficiency or blood transfusion - May cause elevated
RDW results due to iron and/or cobalamin and /or folate deficiency.
PLT (Platelets)
Very small erythrocytes (microcytes), erythrocyte fragments (schizocytes) and WBC fragments may interfere with the proper counting
of platelets and cause elevated PLT counts.
Agglutinated erythrocytes - May trap platelets, causing an
erroneously low platelet count. The presence of agglutinated
erythrocytes may be detected by observation of abnormal MCH and
MCHC values and by careful examination of the stained blood film.
Giant platelets in excessive numbers - may cause an erroneously
low platelet count since these large platelets may exceed the upper
threshold for the platelet parameter and are not counted.
Chemotherapy - Cytotoxic and immunosuppressive drugs may
increase the fragility of these cells which may cause low PLT counts.
Reference (manual) methods may be necessary to obtain an accurate
platelet count.
Hemolysis - Hemolysed specimens contain red cell stroma which
may elevate platelet counts.
A.C.D. blood - Blood anticoagulated with acid-citrate-dextrose may
contain clumped platelet which could depress the platelet count.
Platelet agglutination - Clumped platelets may cause a decreased
platelet count and/or an elevated WBC count. The specimen should
be recollected in sodium citrate anticoagulant to ensure of the
anticoagulated character depending on agglutination and reanalyzed
only for the platelet count. The final PLT result must be corrected for
the sodium citrate dilution effect. However, these platelet clumps do
trigger flags L1, LL and LL1.
MPV (Mean Platelet Volume)
Giant platelets that exceed the upper threshold of the Platelet
parameter may not be counted as platelets. Consequently, these larger
platelets will not be included in the instruments calculation of Mean
Platelet Volume.
page 14/15
1. Specifications
RAA 016 A Ind A

Very small erythrocytes (microcytes), erythrocytic fragments (Schizocytes) and white blood cell fragments may interfere with the proper
counting and sizing of Platelets.
Agglutinated erythrocytes - May trap Platelets, causing an erroneous
MPV result. The presence of agglutinated erythrocytes may be
detected by observation of abnormal MCH and MCHC values and by
careful examination of the stained blood film.
Chemotherapy - May also affect the sizing of PLTs.
IMPORTANT
Blood samples collected in EDTA will not maintain a stable Mean
Platelet Volume. Platelets collected in EDTA swell depending
on the time post-collection and storage temperature.
LYM# (Lymphocyte count absolute value), LYM% (Lymphocyte percentage)

The Lymphocyte count is derived from the WBC count. The presence
of erythroblasts, certain parasites and erythrocytes that are resistant
to lysis may interfere with an accurate LYM count. Limitations listed
for the WBC count pertain to the LYM # and % counts as well.
MON#(mononuclear cell count absolute), MON% (Mononuclear percentage)
The mononuclear cell count absolute is derived from the WBC
count.The presence of large lymphocytes, atypical lymphocytes, blasts
and an excessive number of basophils may interfere with an accurate
monocyte count.
Limitations listed for the WBC count pertain to the MON # and %
counts as well.
NEU#.(neutrophil count absolute), NEU% (Neutrophil percentage)
The neutrophils cell count is derived from the WBC cell count. The
excessive presence of eosinophils, metamyelocytes, myelocytes,
promyelocytes, blasts and plasma cells may interfere with an accurate
neutrophils count.
EOS#.(Eosinophil cell count absolute), EOS% (Eosinophil percentage)
The eosinophil cell count is derived from the WBC cell count. The
presence of abnormal granules (degranulated areas, toxic granules...)
may interfere with the eosinophil counting.
BAS#.(Basophil cell count absolute), BAS% (Basophil percentage)
The Basophil cell count is derived from the WBC cell count.
page 15/15
fhfhf
2. HYDRAULIC & PNEUMATIC

PRINCIPLES
Content
1.
Pentra 60 Hydraulic .............................................................. 2

1.1.
1.2.
1.3.
2.
Transmission tube list .................................................................... 2

Hydropneumatic connections ........................................................ 4
Functions of valves .......................................................................... 7
Pneumatic diagrams ............................................................... 8

2.1.
2.2.
2.3.
2.4.
2.5.
2.6.
2.7.
Pentra 60 general pneumatic diagram ........................................ 8

ABX Alphalyse circuit .................................................................... 9
Eosinofix circuit ............................................................................ 10
Basolyse II circuit ......................................................................... 11
Cleaner circuit ................................................................................ 12
Diluent circuit ................................................................................ 13
Waste circuit .................................................................................. 14
2. Hydraulic & Pneumatic principles
RAA 016 A Ind A
1. PENTRA 60 HYDRAULIC
Liquid circuits, hydropneumatic connections, as well as the transmission tubes used, are described in the following chart tables.
1.1. Transmission tube list
DESIGNAT ION
Y Connector
Y Connector
T410 Connector
T220 Connector
0.040" TY GON tubing
page 2/14
PART
NUMBER
EAB021A
EAB026A
EAB033A
EAB035A
EAE005A
EAE005A
EAE005A
EAE005A
EAE005A
EAE005A
EAE005A
EAE006A
EAE006A
EAE006A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
DIAMET ER
3
2.5
1.6
2.3
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.30
1.30
1.30
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
1.52
LENGT H
QUANT IT Y
10
95
110
130
175
205
240
20
140
320
15
20
50
60
70
80
90
100
120
130
160
200
220
230
250
280
290
300
310
330
335
350
380
1
1
1
8
1
1
1
1
1
2
1
1
1
1
1
1
4
1
4
1
1
1
6
1
1
4
1
1
2
1
1
1
1
1
1
1
1
DESIGNAT ION
Sleevings
Tube shielding
PART
NUMBER
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE007A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE008A
EAE033A
EAE034A
EAE034A
EAE034A
EAE034A
EAE047A
EAE049A
GAL098A
XBA403A
RAA 016 A Ind A
DIAMET ER
LENGT H
QUANT IT Y
1.52
1.52
1.52
1.52
1.52
1.52
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
1.14
2.54
2.54
2.54
2.54
0.19
1.85
400
430
480
500
640
820
20
30
40
55
60
65
70
100
100
120
125
140
140
170
280
300
350
400
420
480
35
20
130
150
340
4
11
1
1
1
2
1
1
9
2
1
1
1
2
2
1
1
5
1
1
1
1
1
1
1
3
1
1
2
1
1
1
1
1
1
25
1
page 3/14
RAA 016 A Ind A
1.2. Hydropneumatic connections

CIRCUIT
AIR
DILUENT
FROM
Atmosphere
EV13_1
Atmosphere
EV15_3
EV15_2
Rinse_2 chamber
(Atmo.)
EV20_1
Diluent Input
EV25_1
Diluent Tanker_1
EV3_3
EV3_2
EV2_1
EV1_1
Isolator_2
T1_2
T1_2
T1_3
T1_3
EV1_2
EV2_2
Injector Syringe_1
LMNE flowcell_7
Diluent Tanker_3
EV9_3
EV9_2
EV10_1
T3_1
EV18_1
Probe Rinsing Body_2
EV17_2
EV18_2
EV10_2
EV21_1
EV22_1
Reagent Heater_8
EV22_2
Reagent Heater_2
EV21_2
page 4/14
F.S.
S
S
S
S
S
S
S
DIAM.
LENGT H T.S.
TO
2.05
1.52
2.05
1.52
1.52
140
70
100
400
70
EV13_2
Counting Syringe_1
EV15_1
Diluent Tanker_2
Counting Syringe_4
2.05
300
EV20_2
1.52
90
Draining Syringe_1
2.05
2.05
2.05
2.05
2.05
1.52
1.52
1.52
1.52
1.14
xba403a
1.14
xba403a
1.52
1.02
1.02
1.02
2.05
2.05
2.05
1.52
1.52
1.52
1.52
1.52
1.52
1.02
1.52
1.52
1.52
1.52
1.52
1.52
1.52
170
140
65
350
120
50
50
200
100
35
EV25_2
Diluent Tanker_4
Diluent Tanker_1
EV3_1
Injector Syringe_2
EV2_3
EV1_3
Isolator_1
T1_1
LMNE flowcell_4
LMNE flowcell_4
LMNE flowcell_2
LMNE flowcell_2
T4_3
Injector Syringe_4
LMNE flowcell_5
(Cap)
Diluent Tanker_3
EV9_1
Diluter Syringe_4
EV10_3
T3_2
EV18_3
Probe Rinsing Body_1
EV17_1
T5_2
Sampling Syringe_2
EV21_3
S
S
S
35
250
175
205
10
65
480
120
50
15
640
330
300
820
110
380
50
200
120
250
120
220
S
S
Reagent Heater_7
RBC chamber_1
Reagent Heater_1
DIL1/HGB chamber_1
Baso/WBC chamber_4
CIRCUIT
FROM
RAA 016 A Ind A
F.S.
DIAM.
LENGT H T.S.
TO
ABX CLEANER Abx Cleaner Bottle

EV7_3
EV7_2
EV12_1
EV12_2
2.05
2.05
1.52
1.52
1.52
400
120
230
350
280
EV7_1
Diluter Syringe_2
EV12_3
Baso/WBC chamber_1
T3_3
ABX
EOSINOFIX
Abx Eosinofix Bottle
2.05
400
EV8_1
EV8_3
EV8_2
Reagent Heater_4
2.05
1.52
1.52
120
500
120
Diluter Syringe_3
Reagent Heater_3
LMNE chamber_3
Abx Basolyse II Bottle
2.05
400
EV11_1
EV11_3
EV11_2
Reagent Heater_9
2.05
1.52
1.52
120
500
60
Reagent Heater_12
1.52
120
Diluter Syringe_5
Reagent Heater_10
Reagent Heater_11
BASO/WBC
chamber_2
Abx Alphalyse Bottle
1.52
290
EV6_1
EV6_3
EV6_2
1.52
1.52
130
480
Diluter Syringe_1
DIL1/HGB chamber_2
SAMPLING
Probe_1
1.02
205
Sampling Syringe_1
LMNE
COUNTING
LMNE chamber_4
1.30
20
M1_2 photocell
M1_1 photocell
EV4_2
T2_3
Injector Syringe_3
EV5_2
T2_1
LMNE flowcell_Output
E1_2 Anode fitting
Isolator_2
E2_2 Ground fitting
EV24_1
T4_1
Reagent Heater_6
1.30
1.02
1.02
1.02
2.05
1.85
0.19
1.52
1.52
1.52
1.52
1.52
1.52
1.52
320
130
240
95
420
11
4
20
70
80
335
120
160
120
EV4_1
T2_2
Injector Syringe_5
EV5_1
T6_2
LMNE flowcell_6
LMNE flowcell_6
E1_1 Anode fitting
Isolator_1
E2_1 Ground fitting
EV24_2
T4_2
Reagent Heater_5
LMNE chamber_2
ABX
BASOLY SE II
ABX
ALPHALY SE
S
S
page 5/14
CIRCUIT
WBC/RBC
COUNTING
DRAININGS
FROM
F.S.
BASO/WBC
chamber_3
EV23_1
RBC chamber_2
EV14_1
EV23_2
Rinse chamber_3
EV27_1
DIL1/HGB chamber_3
EV28_1
T7_1
LMNE chamber_5
EV29_1
T8_1
RBC chamber_4
EV30_1
T9_1
BASO/WBC
chamber_5
EV31_1
T10_3
T6_1
T5_1
M2_2 photocell
Isolator_2
EV26_3
EV26_2
Counting Syringe_5
EV16_2
T11_1
NOTE
page 6/14
RAA 016 A Ind A
S
S
S
DIAM.
LENGT H T.S.
TO
1.52
200
EV23_3
1.52
1.52
1.52
1.52
200
430
70
310
RBC chamber_3
EV14_2
Counting Syringe_2
Counting Syringe_3
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
100
30
70
20
20
70
20
20
60
20
20
EV27_2
T7_3
EV28_2
T7_2
T8_3
EV29_2
T8_2
T9_3
EV30_2
T9_2
T10_2
2.05
55
EV31_2
2.05
2.05
2.05
2.05
2.05
2.05
2.54
2.54
2.05
2.54
2.54
20
30
20
40
20
280
150
130
125
340
20
T10_1
T6_3
T5_3
M2_1 photocell
Isolator_1
EV26_1
Draining Syringe_2
T11_3
EV16_1
T11_2
Waste Output
Read this table as follows in this example :

The Liquid valve 7_2 corresponds to the ouput 2 of the valve number 7
(see attached pneumatic diagram).
RAA 016 A Ind A
1.3. Functions of valves

Valve blocks are located close to the elements concerned.
Five different blocks:
Valves 1 to 11:
On the left side,
Horizontal block,
On the top of the reagent and injection
syringes.
Valves 12 to 16:
On the left side,
Vertical block,
Beside the counting syringe.
Valves 17 to 19:
On the right side,
Horizontal block,
On the sample carriage.
Valves 20 to 26:
On the right side,
Vertical block,
Beside the draining syringe.
Valves 27 to 31:
On the right side,
Horizontal block,
On top of the blending and count
chambers.
page 7/14
RAA 016 A Ind A
The following table shows the functions of each valve in the Pentra
60:
VALVE
NUMBER
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
VALVE SUBJECT
DIL LMNE
DIL LMNE
DIL LMNE
LMNE
LMNE
HEMOLY SE
CLEANER
EOSINOFIX
DIL
DIL
BASOLY SE II
CLEANER
COUNTING SY RINGE
COUNTING SY RINGE
TANK
COUNTING SY RINGE
NEEDLE
NEEDLE
NEEDLE
DRAINING SY RINGE
DIL
DIL
COUNTING SY RINGE
LMNE
DILUENT TANK
FLUSHING SY RINGE
CHAMBER GROUP
CHAMBER GROUP
CHAMBER GROUP
CHAMBER GROUP
CHAMBER GROUP
2. PNEUMATIC
VALVE FUNCT IONS

Directing optical chamber / LMNE chamber
Directing Internal / External sleeving
Control Entry / Exit syringe
Transfer control LMNE tray /Injection syringe
Transfer control LMNE tray /Injection syringe
Directing Circuit chamber / Needle circuit
Directing WBC/BASO chamber / Needle circuit
Set in the atmosphere
RBC/PLT count
Draining
Flushing out of rinsing unit
Commutation Diluent interior/exterior Needle
Reserved
Directing head for WBC count / Valve 22
Directing diluent chamber dil.1
WBC/RBC selection count
Exit from the optical chamber
Entry of the diluent
Flushing of the syringe
Drain rinse chamber
Drain first dilution chamber
Drain LMNE chamber
Drain RBC/PLT chamber
Drain WBC/BASO chamber
DIAGRAMS
2.1. Pentra 60 general pneumatic diagram

See Pneumatic diagram on next page
page 8/14
RAA 016 A Ind A
2.2. ABX Alphalyse circuit
page 9/14
2.3. Eosinofix circuit
page 10/14
RAA 016 A Ind A
RAA 016 A Ind A
2.4. Basolyse II circuit
page 11/14
2.5. Cleaner circuit
page 12/14
RAA 016 A Ind A
RAA 016 A Ind A
2.6. Diluent circuit
page 13/14
2.7. Waste circuit
page 14/14
RAA 016 A Ind A
3. ELECTRIC & ELECTRONIC

PRINCIPLES
Content
1. Pentra 60 Synoptic ...................................................................... 2
2. Pentra 60 Main Board ................................................................. 3
2.1. Main board general view ................................................................... 3
2.2. Main board Test Points ..................................................................... 5
2.3. Main board Potentiometers ............................................................ 7
2.4. Main Board configuration ................................................................. 8
3. Pentra 60 Boards ......................................................................... 9

3.1. Optical preampli board XAA 423 B ................................................ 9
3.2. LCD & Keyboard board XAA 426 B .............................................. 10
3.3. Motor relay board XAA 427 B ..................................................... 11
3.4. Carriage board XAA 428 B ............................................................ 12
3.5. LED board XAA 429 B ..................................................................... 13
4. Connections ................................................................................. 14
4.1. Chamber Heating Connector XBA 386 A .................................... 14
4.2. Reagent Heating Coil Connector XBA 387 A ............................ 15
4.3. Horizontal Carriage Motor XBA 391 A ...................................... 16
4.4. Upper Fan XBA 393 A ..................................................................... 17
4.5. IR sensor XBA 394 AS ................................................................... 18
4.6. IR sensor XBA 395 AS ................................................................... 19
4.7. IR sensor XBA 396 AS .................................................................. 20
4.8. IR sensor XBA 397 AS ................................................................... 21
4.9. RBC / WBC coaxe XBA 398 A ...................................................... 22
4.10. LMNE flowcell coaxe XBA 399 AS .......................................... 23
4.11. Barcode reader cable XBA 402 AS .......................................... 24
4.12. Diluent level sensor XDA 605 AS ............................................. 25
3. Electric & Electronic principles
1. PENTRA 60 SYNOPTIC
page 2/23
RAA 016 A Ind A
RAA 016 A Ind A
2. PENTRA 60 MAIN BOARD

2.1. Main board general view
page 3/23
RAA 016 A Ind A
2.2. Main board Test Points

T EST
POINT
TP1
TP2
TP3
TP4
TP5
TP6
TP7
TP8
TP9
TP10
TP11
TP12
TP13
TP14
TP15
TP16
TP17
TP18
TP19
TP20
TP21
TP22
TP23
TP24
TP25
TP26
TP27
TP28
TP29
TP30
TP31
TP32
TP33
TP34
TP35
TP36
TP37
TP38
TP39
DESIGNAT ION
HB Gain adjustment
PLT Threshold adjustment
LMNE CIS Threshold adjustment
LMNE OD Threshold adjustment
Draining syringe motor
Counting syringe motor
Dilutor syringe motor
Injection syringe motor
UNUSED
Horizontal carriage motor
Sample syringe motor
Needle carriage motor
RBC Threshold adjustment
BASO Threshold adjustment
BASO Comparator
RBC Comparator
PLT Comparator
LMNE CIS Comparator
LMNE OD Comparator
RBC line height adjsutment
BASO gain adjustment
PLT line height adjustment
LMNE Resistive gain adjustment
LMNE Optical gain adjustment
RBC line width adjustment
RBC line reject adjustment
RBC line pulse adjustment
BASO line width adjustment
BASO line reject adjustment
BASO line pulse adjustment
PLT line width adjustment
PLT line reject adjustment
PLT line pulse adjustment
LMNE Resistive line width adjustment
LMNE Resistive line reject adjustment
LMNE Resistive line pulse adjustment
LMNE Optical line width adjustment
LMNE Optical line reject adjustment
LMNE Optical line pulse adjustment
POT ENT IOTARGET T OLERANCE

MET RE
R248
4.7mV
+/- 0.1mV
R159
300mV
+/- 5mV
R160
650mV
+/- 5mV
R161
350mV
+/- 5mV
R149
3V
+/- 50mV
R150
3V
+/- 50mV
R151
3V
+/- 50mV
R152
3V
+/- 50mV
REMARQUE
R154
R155
R156
R158
R157
3V
2V
3V
300mV
300mV
+/- 50mV
+/- 50mV
+/- 50mV
+/- 5mV
+/- 5mV
R133
R134
R135
R136
R148
XX V
XX V
XX V
XX V
XX V
+/ +/ +/ +/ +/ -
50mV
50mV
50mV
50mV
50mV
Factory Adjusted
Factory Adjusted
Factory Adjusted
Factory Adjusted
Factory Adjusted
R142
10s
+/- 0.5s
Factory Adjusted
R139
10s
+/- 0.5s
Factory Adjusted
R144
10s
+/- 0.5s
Factory Adjusted
R145
15s
+/- 0.5s
Factory Adjusted
R147
5s
+/- 0.5s
Factory Adjusted
page 5/23
TP40
TP41
TP42
TP43
TP44
TP45
TP46
TP47
TP48
TP49
TP50
TP51
TP52
TP53
TP54
TP55
TP56
TP57
TP58
TP59
TP60
TP61
page 6/23
5V Power supply
12V Power supply
24V Power supply
-12V Power supply
GROUND
GROUND
GROUND
GROUND
Draining sensor
UNUSED
Reagent heating system CTN voltage
Thermostated room heating sytem
CTN voltage
LMNE Draining sensor
LMNE Lamp
GROUND
GROUND
GROUND
GROUND
GROUND
GROUND
LMNE Resistive line reject2
adjustment
LMNE Resistive line reject2
adjustment
RAA 016 A Ind A
5V
12V
24V
-12V
R286
<1V
With water
R287
R11
<1V
6V
With water
R409
50s
+/- 2s
Factory Adjusted
R410
250s
+/- 5s
Factory Adjusted
RAA 016 A Ind A
2.3. Main board Potentiometers
POT ENT IOMET RE

R133
R134
R135
R136
R139
R142
R144
R145
R147
R148
R149
R150
R151
R152
R154
R155
R156
R157
R158
R159
R160
R161
R248
DESIGNAT ION
RBC line height adjsutment
BASO gain adjustment
PLT line height adjustment
LMNE Resistive gain adjustment
BASO line reject adjustment
RBC line reject adjustment
PLT line reject adjustment
LMNE Resistive line reject adjustment
LMNE Optical line reject adjustment
LMNE Optical gain adjustment
Draining syringe motor
Counting syringe motor
Dilutor syringe motor
Injection syringe motor
Horizontal carriage motor
Sample syringe motor
Needle carriage motor
BASO Threshold adjustment
RBC Threshold adjustment
PLT Threshold adjustment
LMNE CIS Threshold adjustment
LMNE OD Threshold adjustment
HB Gain adjustment
T EST
POINT
TP20
TP21
TP22
TP23
TP29
TP26
TP32
TP35
TP38
TP24
TP5
TP6
TP7
TP8
TP10
TP11
TP12
TP14
TP13
TP2
TP3
TP4
TP1
TARGET T OLERANCE
REMARQUE
Factory
Factory
Factory
Factory
Factory
Factory
Factory
Factory
Factory
Factory
3V
3V
3V
3V
3V
2V
3V
300mV
300mV
300mV
650mV
350mV
4.7mV
Adjusted
Adjusted
Adjusted
Adjusted
Adjusted
Adjusted
Adjusted
Adjusted
Adjusted
Adjusted
+/- 50mV
+/- 50mV
+/- 50mV
+/- 50mV
+/- 50mV
+/- 50mV
+/- 50mV
+/- 5mV
+/- 5mV
+/- 5mV
+/- 5mV
+/- 5mV
+/- 0.1mV
page 7/23
RAA 016 A Ind A
2.4. Main Board configuration
NO
TM
OD
IFY
NO
TM
O
DI
FY
NOTE
page 8/23
DO
DO
If you have initializations problem with 68HC11 Microcontrolers, shut E20

jumper to reset Pentra 60 (instrument must be switched on).
RAA 016 A Ind A
3. PENTRA 60 BOARDS
3.1. Optical preampli board XAA 423 B
OPTICAL PRE-AMPLI BOARD:

Perform an absorbance measurement
This is an Intensity / Tension convertissor
A photodiode measures absorbance of light through the cell, the optical pre-ampli board returns an
intensity proportionnal to the light signal (Light signal is proportionnal to the cell size)
page 9/23
RAA 016 A Ind A
3.2. LCD & Keyboard board XAA 426 B
LCD & KEYBOARD BOARD:

Includes Keyboard and LCD screen (Allows to have one flat connector for all connections)
LED board is connected to this board
page 10/23
RAA 016 A Ind A
3.3. Motor relay board XAA 427 B
MOTOR RELAY BOARD:

This an interconnection board where all the different motor and end of run sensor are connected
This board is connected to the main board with one flat cable
page 11/23
RAA 016 A Ind A
3.4. Carriage board XAA 428 B
CARRIAGE BOARD:
This an interconnection board where all the carriage electrical functions are connected
This board is connected to the main board with one flat cable
page 12/23
RAA 016 A Ind A
3.5. LED board XAA 429 B
LED BOARD:
This board support LEDs on the front panel. They give instrument state:
Red: instrument is busy
Green: instrument is ready to sample
page 13/23
4. CONNECTIONS
4.1. Chamber Heating Connector XBA 386 A
page 14/23
RAA 016 A Ind A
RAA 016 A Ind A
4.2. Reagent Heating Coil Connector XBA 387 A
page 15/23
4.3. Horizontal Carriage Motor XBA 391 A
page 16/23
RAA 016 A Ind A
RAA 016 A Ind A
4.4. Upper Fan XBA 393 A
page 17/23
4.5. IR sensor XBA 394 AS
page 18/23
RAA 016 A Ind A
RAA 016 A Ind A
page 19/23
page 20/23
RAA 016 A Ind A
RAA 016 A Ind A
page 21/23
4.9. RBC / WBC coaxe XBA 398 A
page 22/23
RAA 016 A Ind A
RAA 016 A Ind A
4.10. LMNE flowcell coaxe XBA 399 AS
page 23/23
4.11. Barcode reader cable XBA 402 AS
page 24/23
RAA 016 A Ind A
RAA 016 A Ind A
4.12. Diluent level sensor XDA 605 AS
page 25/23
4. ANALYSIS CYCLE TECHNOLOGY
Content
1.
Analysis cycle description .................................................... 2
2.
Measuring principles ............................................................. 4

2.1.
2.2.
2.3.
2.4.
2.5.
2.6.
2.7.
2.8.
2.9.
2.10.
Multi Distribution Sampling System (MDSS) .......................... 4

RBC / PLT detection principles ................................................... 5
Hgb measurement ........................................................................... 6
Hct measurement ........................................................................... 6
RDW Calculation .............................................................................. 6
MCV, MCH, MCHC Calculation ..................................................... 7
MPV Measurement .......................................................................... 7
Thrombocrit calculation ............................................................... 7
PDW calculation .............................................................................. 8
WBC count and differentiation .................................................. 8
2.10.1.General principles ................................................................ 8
2.10.2.BASO / WBC Count ............................................................ 8
2.10.3.LMNE matrix ........................................................................ 9
2.11. Dilutions summary ......................................................................... 12
4. Analysis cycle technology
1. ANALYSIS
RAA 016 A Ind A
CYCLE DESCRIPTION
Aspiration of blood sample in excess (53l for 5DIFF mode)
Translation of sampling carriage over Rinse chamber.
External rinsing of needle and distribution of the first volume of blood not used (3l).
Translation of sampling carriage over fisrt dilution chamber,

descent of the needle.
Positioning the needle point into a tangential flux of ABX
diluent (1.7ml) and synchronised distribution of 10l of
blood.
Translation of sampling carriage over WBC/BASO count

chamber.
Positioning the needle point into a tangential flux of 2ml of
ABX BASOLYSE II and synchronised distribution of 10l
of blood.
Dilution
Blood Volume
BASOLYSE II Volume
Dilution Rate
10 l
2000 l
1/200
Translation of sampling carriage over LMNE mixing

chamber.
Positioning the needle point into a tangential flux of 1ml of
ABX Eosinofix and synchronised distribution of 25l of
blood.
Dilution
Blood Volume
Eosinofix Volume
Diluent Volume
Final Dilution Rate
page 2/12
25 l
1000 l
1000 l
1/80
RAA 016 A Ind A

Translation of sampling carriage over Rinse chamber
Distribution of the 5l of residual blood during the double
rinsing (interior and exterior) of the needle.
Dilution
Blood Volume
Eosinofix Volume
Diluent Volume
Final Dilution Rate
25 l
1000 l
1000 l
1/80
Translation of sampling carriage over first dilution chamber.

Sample of the 42.5l of the first dilution.
Rinsing of needle exterior (in the first dilution chamber)

with 0.4ml of diluent.
Translation of sampling carriage over RBC and PLT count

chamber.
Distribution of the 42.5l of first dilution into a flux of 2 ml
of diluent.
Distribution of 0.5ml of diluent from the interior of the
needle.
Dilution
Initial Blood Volume
Reagent Volume (RBC chamber)
Final Dilution Rate
10 l
2500 l
1/10000
page 3/12
2. MEASURING
RAA 016 A Ind A
PRINCIPLES
2.1. Multi Distribution Sampling System (MDSS)

The ABX PENTRA 60 analysis cycle needs 3
blood specimens distributed as follows:
one specimen for the first RBC/PLT dilution
and the measure of the HGB.
another specimen for the BASO/WBC count.
the last specimen for the LMNE matrix.
These 3 specimens are provided by means of
a multidistribution principle:
53 l of blood is aspirated inside the needle
(sufficient volume for all the dilutions) then fractions of this specimen is distributed to the
chambers with reagents.
SPECIMENS INSIDE THE NEEDLE
Needle
Reagent
input
Tangential flow
Mixing chamber
BLOOD DISTRIBUTION IN A TANGENTIAL FLOW
page 4/12
Extremities of the specimen are not delivered

to the dilutions.
The specimen distribution in the chambers is

carried out into a tangential flow of reagent
which allows a perfect mixing of the dilution and
prevents from viscosity problems (this multi distribution in a reagent flow is ABX patent).
RAA 016 A Ind A
2.2. RBC / PLT detection principles

Impedance variation generated by the passage of
cells through a calibrated micro aperture.
SOLUTION TO BE ANALYZED
VACUUM = CONSTANT
1 - The specimen is diluted in an electrolytic diluent

(current conductor) and pulled through the calibrated
micro-aperture. Two electrodes are placed on each
side of the aperture. Electric current passes through
the electrodes continuously.
VOLTS
ELECTRODES
ANALYZING
ELECTRONIC
CIRCUIT
2 - When the cell passes through the aperture,

electric resistance between the two electrodes
increases proportionately with the cell volume.
3 - The generated impulses have a very low voltage, which the amplification circuit increases, so
that the electronic system can analyze them and
eliminate the background noise.
TIME
PULSE
Technical characteristics of the RED CELL and PLATELET count:

Dilution
Initial blood volume
Vol. ABX DILUENT
Final dilution rate**
Reaction temperature
Measurement
Method
Ruby diameter
Depression of count
Duration of the count
10 l
2500 l
1/10000
35 C
Impedance
50 m
200 mb
2 X 5 seconds.
** : Two successive dilutions are carried out :

Primary Dilution for RBC and PLT:
Blood (l):
10
ABX DILUENT (L):
1700
dilution:
1/170
Dilution RBC and PLT (from the primary dilution):

dilution (l):
42.5
ABX DILUENT (L):
2500
dilution:
1/58.8
Final dilution: 1/170 X 1/58.8 = 1/10000

Results
Number of cells counted per volume unit x Calibration coefficient
Histograms
RBC: Distribution curves on 256 counting channels from 30fl to 300fl.
PLT: Distribution curves on 128 channels from 2fl to a mobile threshold.
This threshold moves according to the microcyte population present
in the analysis area.
page 5/12
RAA 016 A Ind A
2.3. Hgb measurement

The hemoglobin freed by the lysis of the red blood cells combines
with the potassium cyanide to form the chromogenous
cyanmethemoglobin compound.
The compound is then measured by spectrophotometry, through the
optical part of the first dilution chamber, with a wave length of 550 nm.
Technical characteristics for the measurement of the hemoglobin:
Dilution
Volume of blood
Volume ABX DILUENT
Volume ABX ALPHALYSE
Complement ABX DILUENT
Final dilution rate
Measurement
Method
Wavelength
10 l
1700 l
400 l
400 l
1/250
35 C
Photometry
550 nm
Result
Absorbance value obtained x coefficient of calibration
2.4. Hct measurement

The height of the impulse generated by the passage of a cell through
the micro-aperture is directly proportional to the volume of the analyzed
RBC.
The hematocrit is measured as a function of the numeric integration
of the MCV.
2.5. RDW Calculation

The study of the RBC distribution detects erythrocyte anomalies linked
to anisocytosis.
A Red Cell Distribution Width (RDW) will enable you to follow the
evolution of the width of the curve in relation to the cell number and
average volume.
K SD
RDW =
MCV
With
K = system constant
SD = Determined standard deviation according to statistical
studies on cell distribution.
MCV = Mean Corpuscular Volume of erythrocytes
page 6/12
RAA 016 A Ind A
2.6. MCV, MCH, MCHC Calculation

- MCV (Mean Cell Volume) is calculated directly from the RBC
histogram.
- MCH (Mean Cell Hemoglobin) is calculated from the HGB value and
the RBC number.
The mean hemoglobin weight in each RBC is given by the formula :
HGB
MCH (pg) = --------- x 10
RBC
- MCHC (Mean Corpuscular Hemoglobin Contained) is calculated
according to the HGB and HCT values. Mean HGB concentration in
the total volume of RBC is given by the formula :
HGB
MCHC (g/dL) = ------- x 100
HCT
2.7. MPV Measurement

The MPV (Mean Platelet Volume) is directly derived from the analysis
of the platelet distribution curve.
2.8. Thrombocrit calculation

Thrombocrit is calculated according to the formula:
Plt (103/l) x MPV (m3)

PCT% =
----------------------------10 000
page 7/12
RAA 016 A Ind A
2.9. PDW calculation

PDW (Platelet Distribution Width) is calculated from the
PLT histogram.
The PDW is represented by the width of the curve between
15% of the number of platelets starting from 2 fl (S1) and
15% of the number of platelets beginning with the variable
top threshold (S2).
2.10.
WBC count and differentiation
2.10.1. General principles

The WBC count is carried out two times by two different sensors:
In the BASO count chamber at the same time as the BASOS count.
In the optical chamber during the acquisition of the LMNE matrix
(available from the V1.1 software version)
The reference count is the one obtained in the WBC and BASOS
count chamber.
2.10.2. BASO / WBC Count

Detection principles is the same as for RBC.
Differentiation betwen BASOs and other leukocytes is obtained by
means of the new BASOLYSE II specific lysing action.
The basophils are located from threshold <2> to the

end. 100 % of the leukocytes are represented by the
total number of nucleic particles plus the basophils from
the threshold <1> to <3>.
Percentage of basophils is calculated from the number
of particles existing from the threshold <2> to <3>.
page 8/12
RAA 016 A Ind A
Technical Characteristics of the WBC/BASO count:

Dilution
Volume of blood
Volume of BASOLYSE II
Rate of dilution
10 l
2000 l
1/200
35 C
Measurement
Method
Ruby diameter
Depression of count
Duration of the count
Impedance
80 m
200 mb
2 X 6 seconds
Results
WBC: Number of cells per volume x coefficient of calibration.
BASO: Number of cells per volume x coefficient of calibration in
percentage regarding the number of counted cells (BASO + WBC
nuclei)
2.10.3. LMNE matrix

The WBC matrix analysis of the ABX PENTRA 60
is based on 3 essential principles:
1- The double hydrodynamic sleeving: DHSS
(ABX patent)
2- The volume measurement : impedance changes.
3- The measurement of transmitted light with 0
angle, which permits a response according to
the internal structure of each element and its
absorbance by means of incident light diffusion.
25l of whole blood is delivered to the LMNE
chamber in a flow of EOSINOFIX. This reagent lyses the RBC, stabilizes the WBC in their native
forms and stains the eosinophil nuclei with a specific
coloration.
The solution is then stabilized with diluent and
transferred to the measuring chamber. Each cell is
measured both in absorbance (cytochemistry) and
resistivity (volume).
page 9/12
RAA 016 A Ind A
Technical characteristics of the WBC count during the acquisition of the matrix:
Dilution
Volume of blood
Volume Eosinofix
Volume diluent
Rate of final dilution
Incubation duration
25 l
1000 l
1000 l
1/80
35 C
12 s.
Measurement
Method
Ruby diameter
Diameter of the flow
Injection duration
Volume injected
Impedance with hydrofocus

60 m
42 m
12 seconds
72 l
Results
From these measurements, a matrix is drawn
up with volumes on the X-axis and optical transmission on the Y-axis
The study of the matrix image permits the clear
differentiation of 4 out of 5 leukocytes populations. As a matter of fact, the basophil population is very small compared to the other 5 in a
small blood sample.
EOSINOPHILS: With reagent action on cytoplasmic membranes, the

leukocytes keep their native size and only eosinophils are colored for
optical separation.
Eosinophils will be situated in the upper part of the optical Y-axis due
to their strong absorbance qualities and their size, which is nearly
equivalent to large neutrophils.
NEUTROPHILS: The neutrophils, with their cytoplasmic granules and

their generally segmented nuclei, will scatter light depending on their
morphological complexity. A hypersegmented neutrophil will give an
increased optical response with respect to a young neutrophil population which will be in the upper position of the optical axis depending
on the presence of segmentation and/or granules.
page 10/12
RAA 016 A Ind A

LYMPHOCYTES: The lymphocytes being small with regular shape,
are positioned in the lower part of both the optical axis and volume
axis.
Normal lymphocyte populations are generally observed with a good
volume homogeneity and with a gaussian distribution.
Large lymphocytes are detected in the ALY (Atypical Lymphocytes)
zone, where reactive lymphoid forms, stimulated lymphocytes and
plasmocytes are also to be found.
The far left side of the lymphocyte zone should normally be empty, but
when small lymphocytes are present, population may exist in this area.
The presence of platelet aggregates is detected by a distribution pattern that moves from the origin of the matrix (background zone) into
the lymphocyte zone.
The NRBCs having their cytoplasmic membranes lysed like those of
the erythrocytes, will have their nuclei situated to the far left side of
the lymphocyte zone.
MONOCYTES: The monocytes, being cells with large kidney shaped
nuclei and a large non-granular cytoplasm, will neither be scattered
nor absorb a large amount of light. They will therefore be positioned in
the lower part of the optical axis but clearly to the right of the volume
axis.
Certain large monocytes can be found on the right side of the matrix
in the lower LIC (Large Immature Cells) zone.
The immature granulocytic cells are detected by their larger volumes
and by the presence of granules which increase the intensity of the
scattered light.
Therefore, cells such as metamyelocytes will be found clearly to the
right of the neutrophils and nearly at the same level.
Myelocytes and promyelocytes will be found in saturation position on
the far right of the matrix. These last three populations will be counted
as LIC (Large Immature Cells) and their given results are included in
the neutrophil value.
The blast cells will be found generally to the right of the monocytes,
and, as such, will increase the LIC count. Small blasts will be found
between the normal lymphocytes and monocytes.
Platelets and debris from erythrocyte lysis represent the background
noise population located in the lower left area of the matrix.
Most of the population partition thresholds are fixed and give the limits
of the morphological normality of leukocytes. Changes in the
morphology of a population will be expressed on the matrix by a shifting
of the corresponding population.
page 11/12
RAA 016 A Ind A
2.11. Dilutions summary
T ECHNICAL
CHARACT ERIST ICS FOR
BLOOD
VOLUME
REAGENT
WBC Count in WBC/BASO

Chamber
10l
BASOLY SE II
2000l
1/200
35C
WBC Count during acqu sition

of LMNE matr xi
25l
EOSINOFIX
DILUENT
1000l
1000l
1/80
35C
BASOPHIL Count
10l
BASOLY SE II
1000l
1/200
35C
RBC and PLT Count
10l
DILUENT
2500l
1/10000
35C
Hemoglobin Measurement
10l
DILUENT
ALPHALY SE
DILUENT (Comp .l)
1700l
400l
400l
1/250
35C
page 12/12
REAGENT FINAL DILUT ION REACT ION

VOLUME
RAT E
T EMP.
5. SOFTWARE VERSIONS
Content
1. Software Overview ..................................................................... 2
1.1. User Menu Overview ............................................................................... 2
1.2. Technician Menu Overview .................................................................... 3
2. Software Release ........................................................................ 4

2.1. Software Release Table ....................................................................... 4
2.2. Software Modifications ........................................................................ 4
5. Software versions
RAA 016 A Ind A
1. SOFTWARE OVERVIEW
1.1. User Menu Overview
1-START CYCLE
2-CALIBRATION
3-REAGENTS
1-DILUENT
2-CLEANER
1-AUTOCAL.
1-LEVEL/CHANGE
2-CHANGE COEFF.
2-DAILY
WORKLOAD
3-EOSINOFIX
3-PRINT COEFF.
3-PRIME
4-BASOLYSE II
5-ALPHALYSE
6-ALL REAGENTS
4-MAINTENANCE
1-BACK FLUSH
1-CHAMBERS
2-RINSE
2-CYTOMETER
1-RINSE
1-AUTOCONTROL
3-DRAIN CHAMBER
2-HYDRAULICS
SYSTEMS
2-FIRST DILUTION
1-SAMPLINGNEEDLE
4-CONCENTRATED
CLEANING
3-MECHANICAL
SYSTEMS
3-LMNE
2-CARRIAGE
1-1 TO 11
4-WBC/BASO
4 - TECHNICIAN
1-INITIALIZATION
2-12 TO 16
3-17 TO 19
3-SAMPLING SYR.
4-DRAINING SYR.
4-RBC/PLT
2-CHECK MOTORS
4-20 TO 26
3-CHECK VALVES
5-COUNTING SYR.
5-ALL CHAMBERS
5-27 TO 31
6-CYTOMETER SYR.
6-DILUENT RESERVOIR
7-DILUTION SYR.
5-SETUP
1-DATE FORMAT
1-DD/MM/YY
2-SET DATE/TIME
2-MM/DD/YY
1-NORMAL RANGES
1-CBC
1-DATE/TIME
3-YY/MM/DD
2-PANIC RANGES
2-UNITS
2-DIFF
1-STANDARD
3-FLAG SENSITIVITY
1-CBC
2-S.I.
2-DIFF
4-THRESHOLDS
3-mmol
3-LAB. LIMITS
1-RS232
CONFIGURATION
4-RS232
1-PRINTER
CONFIGURATION
2-SENDING
CONFIGURATION
2-PRINT LATEST
RESULT
3-ABX FORMAT
5-PRINTER
1-CALIBRATION
2-IDENTIFICATION
MODE
3-AUTOCLEAN
FREQUENCY
4-SEND LATEST
RESULT
1-CV% LIMITS
1-NUMERICAL RESULTS
2-FLAGS &
PATHOLOGIES
3-HISTOGRAMS &
THRESHOLDS
2-DEFINE OPERATOR
4-PATIENT FILE
4-CHANGE PASSWORD
6-OTHERS
5-LANGUAGE
1-ENGLISH
6-REAGENT
CAPACITIES
2-FRENCH
5-RAW DATA
MENU PENTRA 60
page 2/4
RAM 048 AA
7-CYCLES #
RAA 016 A Ind A
1.2. Technician Menu Overview
4-MAINTENANCE
1-DILUTION
1-AUTOCONTROL
2-HYDRAULICS
SYSTEMS
3-MECHANICAL
SYSTEMS
1-Hb BLANK
ADJUSTMENT
2-APPERTURE
CURRENT
2-MEASUREMENT
3-RBC/PLT GAIN
3-HEATING
SYSTEMS
4-WBC/BASO GAIN
5-LMNE
ADJUSTMENT
4-TECHNICIAN
4-BUBBLING
1-REAGENT
HEATING SYSTEM
1-ADJUSTMENT
2-AMBIANCE BOX
2-REFERENCE
1-DRAINING
1-ADJUSTMENT
2-LMNE TRANSFER
2-REFERENCE
5-SENSOR CHECK
6-VACUUM CHECK
2-BURN-IN
1-COUNTING
2-DRAINING
8-USER MODE
1-CYCLES #
9-OTHERS
2-MAINTENANCE
CARRIAGE POSITION
3-PARK SYRINGES
POSITION
MENU PENTRA 60
RAM 059 AA
4-SPECIAL VALUES
page 3/4
RAA 016 A Ind A
2. SOFTWARE RELEASE
2.1. Software Release Table
Software Pentra 60
Release Serial Number
Note
V0.82
60 002 to 60 010
First release,
Not on field
V1.02
60 011 to 60 090
More utilities, Default values changed,

No change on cycle and Results reliability
V1.04
60 091 to ...
New basophil flag management 1,

No change on cycle and Results reliability
2.2. Software Modifications

V1.04: 1 A flag MB (Mono Baso) is triggered off when the percentage of
basophils found on the Baso channel is above the percentage of lympho/
mono/neutro raw count found on the matrix channel.
If the BASO value is higher than 50%, a BASO+ flag is triggered
off. The Basophils are not taken away from the matrix populations
and (--,--) are displayed and printed out instead of the Baso
percentages and absolute values.
page 4/4
6. OUTPUT FORMAT
Content
1.
Argos format principles ....................................................... 2

1.1.
1.2.
1.3.
1.4.
2.
ABX format principles .......................................................... 5

2.1.
2.2.
2.3.
2.4.
2.5.
2.6.
2.7.
2.8.
3.
Introduction ..................................................................................... 2
Results characteristics ................................................................. 2
1.2.1. Key ........................................................................................... 2
1.2.2. Result format ....................................................................... 3
Patient file characteristics .......................................................... 4
1.3.1. Key ........................................................................................... 4
1.3.2. Patient file format .............................................................. 4
End of communication ................................................................... 4
1.4.1. Key ........................................................................................... 4
Message structure ......................................................................... 6

Details about the structure ........................................................ 6
Identifier list and their formats ............................................... 7
2.3.1. Hematology numerical parameters ................................ 7
2.3.2. Identifier list ....................................................................... 8
Pathology ........................................................................................... 9
2.4.1. Flags associated with parameters .................................. 9
2.4.2. Pathological messages ...................................................... 10
Histograms and matrix ................................................................ 13
2.5.1. Format description ............................................................ 13
2.5.2. Format description of the threshold transmission .. 14
Patient result identification ...................................................... 18
2.6.1. Format description ............................................................ 18
2.6.2. Identifier list ..................................................................... 18
2.6.3. Analysis type ($80) ........................................................... 19
Packet type .................................................................................... 19
2.7.1. Data exported by the analyzer ..................................... 19
2.7.2. Data interpreted by the analyzer ............................... 20
Other identifiers ......................................................................... 20
Pin assignments ..................................................................... 21
6. Output format
RAA 016 A Ind A

3 RS ouput formats are available on PENTRA 60 and they are identical
to PENTRA 120:
- Format 1 : ARGOS / HELIOS type
- Format 2 : ABX internal format (factory use)
- Standard format
- TR off : Transmission off
1. ARGOS
FORMAT PRINCIPLES
1.1. Introduction
The ARGOS format is a fixed format (406 characters for one result)
including a STX and a ETX. These characters are splitted into fields
representing a transmitted item.
406 CHARACTERS
STX
DATA
ETX
The data transmitted can be a result :

STX
"R" / ANALYSER # / NID / ID / etc...
CRC ETX
or a patient file :
STX
"D" / ANALYZER # / NID / ID / etc...
CRC
ETX
CRC
ETX
or end of communication :
STX
"E" / ANALYSER # / ...
The fields have a fixed length separated by the OD character.
1.2. Results characteristics

1.2.1.
Key
Total ASCII characters emitted : 406
- (-) : Space $20
- (]) : Carriage return $0D
- CRC : exclusive "OR" of all the transmitted bytes except ETX and
STX, then an inclusive "OR" with a $4O value.
- zzzzz : numeric field completed by zeros on the left.
ex : 04.55 (decimal separation with a period).
When the analyser does not transmit parameters, the field
(zzzzz) is put in place of (--.--).
page 2/21
6. Output format
RAA 016 A Ind A

- Y : Alphanumeric character from $20 to $7F.
- # : Space ($20) if automatic sampling. Star ($2A) if manual sampling.
1.2.2.
Result format
page 3/21
6. Output format
RAA 016 A Ind A
1.3. Patient file characteristics

1.3.1.
Key
- (]) :
Carriage return $OD.
- CRC :
Exclusive OR of all the transmitted bytes, except ETX

and STX, then the inclusive OR with a $40 value.
- Y, Z :
1.3.2.
Alphanumeric character from $20 to $7F.
Patient file format
Table 2
1.4. End of communication

1.4.1.
Key
- (]) : Carriage return $0D
- CRC :the exclusive OR of all the transmitted bytes, except ETX
and STX, then the inclusive OR with a $40 value.
- zz : Number of the analyser.
page 4/21
6. Output format
1.4.2.
RAA 016 A Ind A
Line free format
Table 3
2. ABX
FORMAT PRINCIPLES
The ABX format can have a different number of fields according to
the transmitted items setup by the user (results, curves, flags, etc...).
The fields have the same characteristics than the ARGOS format. The
result identifier is different according to the type of result : patient
result ("RESULT"), re-run result (RES-RR), QC result (QC-RES) etc...
Example of "abx format":
STX
$OD
$FF
SPACE
ID
SPACE
ID
SPACE
ID
SPACE
$FE
SPACE
$FD
SPACE
PACKET LENGTH
(without STX-ETX)
U
$OD
$OD
FIELD 1
$OD
$OD
$OD
PACKET TYPE
$OD
FIELD 2
FIELD n
VARIABLE
THE ABX F
(Fonction of the
FORMAT VERSION
NUMBER
CHECKSUM
ABX FOR
POST-HEA
ETX
Diag.17
page 5/21
6. Output format
RAA 016 A Ind A
2.1. Message structure

- STX
- Size + carriage return.
- Identifier followed by a Load Type + carriage return.
- Identifier followed by the Information associated to the Load Type +
carriage return.
- Remainder of the other Identifiers and Informations associated to
the Load Type + carriage returns.
- Other Load Type blocs + Associated Informations.
....................................................................................
- Identifier followed by a space then the CheckSum + carriage return.
- ETX
2.2. Details about the structure

Size : 5 bytes representing the total amount of the data except STX
and ETX.
Load : An 8 character chain preceeded by a space indicating that
this load is a result type.
Identifier : 1 byte (moving about $21 to $FF, it describes the information type which follows this indicator).
CheckSum : Sum modulo 65535 of all the characters except ETX ,
STX and all informations about checksum (identifier - space checksum - carriage return) in the hexadecimal format on 4 bytes,
preceeded by a space.
NOTE
page 6/21
Strings of characters having a length smaller than the expected character

number have to be completed on the right handside by "spaces".
6. Output format
RAA 016 A Ind A
2.3. Identifier list and their formats

2.3.1.
Hematology numerical parameters

A - Format description
Numerical field :
For all indicated parameters from $21 to $43, the format is a numerical
field of 5 digits completed with zeros on the left side (ex.: 04.55) and
preceeded by a space.
The unit is the standard units.
When the parameter cannot be calculated by the analyzer, the field is
replaced with (--.--).
Parameter status :
Following the numerical field, a first digit gives the counting rejection
status or the suspicion, a second one gives the parameter value status
according to high and low normalities, to high and low extreme values
and to the overloading capacities.
Table 4
Example :
5.5 millions RBC with a counting error in the standard units :
$32 $20 $30 $35 $2E $35 $30 $52 $68 $0D ou 2 05.50Rh +
carriage return.
The length is fixed and is worth 2+7+1, that is to say 10 bytes for one
parameter.
page 7/21
6. Output format
2.3.2.
Table 5
page 8/21
Identifier list
RAA 016 A Ind A
6. Output format
RAA 016 A Ind A
Table 6
Table 7
2.4. Pathology
2.4.1.
Flags associated with parameters

A. Format description
Flags are transmitted in a comprehensive mode (same presentation
than on the screen, that is to say dependant from the language),
preceeded by a space. They are replaced with spaces when the flag
has not been detected.
page 9/21
6. Output format
RAA 016 A Ind A
B. Identifier list (english)
Table 8
C. Identifier list (french)
Table 9
NOTE
- Pathologies for the differential are given in capital letters for the english
language in order to avoid confusion between L1 and LL.
- Pathology for platelets "Pc" is given for small cells (Petites cellules in french)
in order to avoid confusion with "Sc" (Schizocytes).
Example of flags on WBC balance :
2.4.2.
- $66 $20
BASOLmne+BASO+ $0D
- $66 $20
WBC1 $20$20$20$20$20$20$20$20$20$20 $0D
- $66 $20
$20$20$20$20 Lmne- $20$20$20$20$20 $0D
Pathological messages
Each pathology is described by a group of 4 letters preceded by a
space. The content of the pathology is dependent from the chosen
language. Messages are divided in 3 groupes. Only the detected pathologies are transmitted. A common header to the 3 groups indicates
that the pathological interpretation has not been determined by the
analyzer.
page 10/21
6. Output format
RAA 016 A Ind A
A. Format description (english)
Table 10
Table 11
NOTE
Levels 1, 2, and 3 are not available with the V2.2 version.
Table 12
Table 13
Table 14
page 11/21
6. Output format
RAA 016 A Ind A
B. Format description (french)
Table 15
Table 16
NOTE
Table 17
Table 18
page 12/21
Levels 1, 2, and 3 are not available with the V2.2 version.
6. Output format
RAA 016 A Ind A
Table 19
B. Identifier list
Table 20
2.5. Histograms and matrix

2.5.1.
Format description
A. Histograms
Histograms are transmitted on 128 channels, preceeded by a space.
They are automaticaly rescaled to a 223 maximum amplitude value.
The zero amplitude value is $20, the maximum amplitude value is $FF.
B. Matrix
- SCREEN BITMAP : 2048 graphic bytes matrix are transmitted on
4096 ASCII bytes, preceeded by a space. Each group of 2 ASCII
bytes is the value of 1 graphic byte represented from the left to the
right and from the top to the bottom of the screen (principles of the
recovery of the 128x16 matrix bitmap on the PENTRA 120 LCD board).
The double matrix [127 (ABS) x 256 (RES)] is transmitted according
to the same procedure.
ABS ou OFL (Y)
(n de canal)
127
octet 1
octet 2
octet 16
ligne 1
octet 17
octet 18
octet 32
ligne 2
Mode de transmission d'un octet

octet
octet
poids forts
BITMAP de la MATRICE Lmne ou Rticulocytes
octet
poids faibles
dcalage droite
de 4 bits
ET logique
avec $ 0F
Transformation ASCII
octet 2033
octet 2034
octet 2048
ligne 128
octet A
ASCII poids forts
octet B
ASCII poids faibles
RES ou CIS (X) (n de canal)

0
127
Diag.18
page 13/21
6. Output format
RAA 016 A Ind A

EXTENDED MODE FORMAT (3D curve) : The extended format
includes all the heigth informations relative to each chanel. It is
constituted by a chart of 128 [ABS] x 128 [RES], 128 [ABS] x 256
[RES] or 256 [OFL] x 128 [CIS] entries of 16 bytes each. These 32K
or 64K charts are first of all compressed (3k approximately for a 32K
chart) then encoded (expansion 1 to 2) before being transmitted. The
format is as follow : identificator, space, compression algorythm on 8
characters ("compress" or "gzip" or "lha" or "squeeze"), space,
encoding type on 8 characters (od or uuencode), chart format on 8
characters, space, encoded data size on 5 characters, space, data
then carriage return.
EXAMPLES :
$64 $20 lha$20$20$20$20$20 $20 od$20$20$20$20$20$20 $20 128Y256X $20 10772 $20 data $0D
$65 $20 compress $20 uuencode $20128X128Y $20 05386 $20 data $0D
Only the "squeeze" compression mode and the "uuencode" encoding mode
are now available
NOTE
C. Separation thresholds
It is the channel number (decimal value) enclosing areas on the
histograms or on the matrix. Each threshold is transmitted on 3 bytes
preceeded by a space
2.5.2.
Format description of the threshold transmission
Diag.19
2 3
L1
A. WBC identifier
Separation thresholds 1-2-3 allow the L1 flag determination. For the
analyzers in LMG mode, the 4-5 thresholds allow the separation of the
3 populations Lymphocytes, Monocytes, Granulocytes.
Example 1 : output format of the WBC curve thresholds for an LMG
sampling. In this example, the analyzer doesnt send the calculation
thresholds of the L1 flag.
] 000 000 000 040 060 + carriage return.
page 14/21
6. Output format
RAA 016 A Ind A

Example 2 : output format of the WBC curve thresholds for CBC and
DIFF sampling. In this 5parts DIFF analyzer, the LMG is not measured,
the 4-5 thresholds are not significants.
] 005 008 020 000 000 + carriage return.
RBC1
RBC2
%MIC
%MAC
Diag.20
B. RBC identifier
Separation thresholds 1-2 allow the calculation of the microcytic and
macrocytic cell proportions.
PLT1
PLT
Diag.21
C. PLT identifier
The threshold 1 is the number of the last channel used to calculate the
PLT number.
BA1
BA3
BA2
BASO
Diag.22
D. Basophil identifier
Thresholds 1-2-3 allow the determination of the basophil proportion
regarding the total number of WBCs.
page 15/21
6. Output format
RAA 016 A Ind A
E. Lmne matrix identifier

The 12 resistive thresholds are transmitted in the following order :
NoL, NoN, NoE, LN, RN, LL, AL, LMU, LMD, LMN, MN, RM. The 3
absorbance thresholds are following : NL, NE, RMN. At the end the
width of the areas (in channel number) describing the proximity flags
: FNE, FMN, FLN are transmitted .
Areas surrounded by bolt lines give the matrix populations. Names of
the populations are shown in italic. Hatched areas give the sub populations and belong to the main population.
Framed items are the proximity flags. Surrounded items are inflexion
points similar to separation thresholds.
NoN
Absorbance
FMN
NoE
Channel 127
EOS
FNE
NOISE
NE
LEFT
NEU
FLN
RN
NEUTRO
LN
RMN
MONO
NL
LEFT
LYM
NoL
RIGHT
NEU
ATY
LYM
LYMPHO
LL
LMD
AL
LMN
RIGHT
MON
RM
Resistivity
MN
LMU
English
Absorbance
AMN
BfE
BfN
Canal 127
EOS
ANE
BRUIT de FOND
NE
NEU
gauche
ALN
NG
NEUTRO
MND
MONO
NL
LYM
gauche
BfL
page 16/21
French
LYM
aty.
LYMPHO
LG
LMB
LA
LMN
Diag.23
ND
NEU
droit
LMH
MON
droit
MD
Rsistivit
MN
6. Output format
RAA 016 A Ind A
F. Reticulocyte matrix identifier

The 2 resistive thresholds are transmitted in the following order : R1,
R2, R3, R4.
Then the 4 fluorescent thresholds are following : F1, F2, F3, F4.
O.F.L
F4
F3
F2
F1
R1
R2
R3
R4
C.I.S
Diag.24
8.3.5.3. Identifier list
Table 21
page 17/21
6. Output format
RAA 016 A Ind A
2.6. Patient result identification

2.6.1.
Format description
All the described fields have a fixed size character string type and are
completed with spaces for the non significant informations.
2.6.2.
Table 22
page 18/21
Identifier list
6. Output format
NOTE
2.6.3.
RAA 016 A Ind A
- The date format ($71, $77, $7D) depends on the date format setup by the user.
- $78 : the strings smaller than the expected charcter number have to be completed on
the right handside by "spaces".
- There is no check on $77 compare to $78 which is involved into the automatic type
selection $7f
Analysis type ($80)

This identifier allows to identify the analysis type : CBC, DIF, RET,
LMG to be carried out on the sample . It gives also the access to the
analysis of one or several specific parameters. The CBC analysis
includes the 12 CBC's parameters. The DIF analysis includes the
CBC analysis and the 14 results in % and # of the WBC sub-populations. The RET analysis gives access to the 5 results in % and #. The
analysis of one or several specific parameters can be set up by the
user. The LMG analysis includes the CBC analysis and the 6 results
in % and # of the WBC sub-populations.
FORMAT : $80 $20 one character from 'A' to 'F' $0D
2.7. Packet type

The information described in the packet type allows the specification
of the global message content : hematological routine results or statistic
results.
This string of characters has a length is 8 characters, preceeded by
a space resumming the following informations. The identifier of this
packet type is : $FF.
Table 23
2.7.1.
Data exported by the analyzer
Table 24
Example : The packet type 'RESULT' will be followed by hematological
parameters or matrix and histograms.
page 19/21
6. Output format
2.7.2.
RAA 016 A Ind A
Data interpreted by the analyzer

The 'FILE' packet corresponds to the file reception, it is followed with
a part or all the patient file. This command is transmitted in the same
format and according to the general structure of the message : identifier $FF, space, packet type then carriage return.
This packet type allows the worklist setup.
Table 25
2.8. Other identifiers

Identifier $FB : identifies the analyzer type when communicating.
Identifier $FC : This identifier allows the transmission of a number
which can be an error number,
a position number, a
burn-in sequence number or a status in hexadecimal mode
(Not available yet).
Identifier $FD : Checksum value (see section on the message structure).
Identifier $FE : The version number is linked to the development of the
hematological
message identifiers.
NOTE
Identifier $FC The error list is dedicated to the "remote" mode, however the
MICROS instrument is able to transmit the error #0001 (Operating temperature
out of limits).
Table 26
For $FB and $FF, the strings having less than 8 characters are completed on
the right handside by "spaces".
NOTE
Analyzer name list :
Table 27
page 20/21
6. Output format
3. PIN
RAA 016 A Ind A
ASSIGNMENTS
page 21/21
7. ALARMS & ERROR LIST
Content
1.
Error messages ...................................................................... 2

1.1.
1.2.
1.3.
1.4.
1.5.
1.6.
Printer ................................................................................................ 2
Transmission ..................................................................................... 2
Calibration ......................................................................................... 2
Technical Operations ..................................................................... 3
Reagents ............................................................................................ 3
Temperature .................................................................................... 3
7. Alarms & Error list
1. ERROR
RAA 016 A Ind A
MESSAGES
1.1. Printer
DISPLAYED MESSAGES
CAUSES
USER ACTIONS
The printer is disconnected, switched

off or has not been selected
Printout operations
disabled
- Switch on or
- press "ON LINE"or
- see the printer user's manual
Defect on printer, make sure there is

paper
Printout operations
disabled
- F eed paper or
- see the printer user's manual
1.2. Transmission
DISPLAYED MESSAGES
CAUSES
USER ACTIONS
No ENQ character received on RS232

No ACK character received on RS232
Read error on RS232
Write error RS232
defect on
transmission
operations
Timeout overflow on RS232
Check the RS 232 configuration

Menu "SETUP / RS 232 / RS232
CONFIGURATION"
Call ABX representative service
department
Result transmission impossible

Transmission mode &/or format
incompatible
1.3. Calibration
DISPLAYED MESSAGES
page 2/3
CAUSES
USER ACTIONS
Coefficients outside the limits
Coefficients out of the

ranges given section 3
Perform an autoconcentrated cleaning

Run another calibrator vial
Data not saved, value out of range
incoherent value entered

by the operator
Re-type in the item
Illegal date
Incoherent date entered

by the operator
Re-type in the date
Minimum tagged CBC incorrect, at

least 3
Selected results for

calibration calculation < 3
Select at least 3 results
7. Alarms & Error list
RAA 016 A Ind A
1.4. Technical Operations

DISPLAYED MESSAGES
Emergency stop, Run an autocontrol
CAUSES
Blocked motor
Incorrect Drains
Thermal door opened
USER ACTIONS
control the motor operations : Menu

"MAINTENANCE / MECHANICAL
SYSTEMS / CHECK MOTORS"
.....not reaching home
Blocked motor
Thermal door opened
open during a cycle
close the door and rerun the cycle
Illegal time
Incoherent time entered

by the operator
Enter the correct time
1.5. Reagents
DISPLAYED MESSAGES
E FFE C TS
USER ACTIONS
No diluent, check level
diluent reservoir empty
replace the diluent container

(menu "REAGENTS/LEVELCHANGE")
Reagent low level (reagent nam e)
none
Replace the bottle (menu

"REAGENTS/LEVEL-CHANGE")
Reagent low level
Message triggered at the

end of the Startup
control the reagent levels or /and

replace it.
1.6. Temperature
DISPLAYED MESSAGES
CAUSES
Temperature out of range
Thermic regulation problem
Heating coil initialization
Operating temperature not

reached
USER ACTIONS
Call ABX representative
department
Wait for a few minutes
page 3/3
Content
8. MAINTENANCE
1.
Maintenance ............................................................................. 2
1.1.
1.2.
1.3.
1.4.
2.
Maintenance kits ..................................................................... 4

2.1.
2.2.
2.3.
2.4.
2.5.
3.
Introduction ..................................................................................... 2
Daily customer maintenance ........................................................ 2
Weekly customer maintenance ................................................... 2
Maintenance table .......................................................................... 3
6 month Maintenance kit .............................................................. 4

Yearly Maintenance kit ................................................................. 5
Pistons Maintenance kit ................................................................ 6
Fitting Kit .......................................................................................... 7
Screws Kit ......................................................................................... 8
Procedures ............................................................................. 10
3.1.
3.2.
Procedure chart tables ............................................................... 11

Required tools and products ..................................................... 12
8. Maintenance
RAA 016 B Ind A
1. MAINTENANCE
1.1. Introduction
WARNING !
Customer maintenance has to be carried out according to the recommended frequency
chart table and after having attended an ABX approved customer training course.
The system warranty may be affected if damage occurs after a non trained technician
intervenes or if replaced spare parts and consumables do not come from an ABX
approved origin.
1.2. Daily customer maintenance

No special adjustments or maintenance has to be done on your
equipment if the recommended startup and shutdown procedures are
explicitly respected.
See the ABX PENTRA 60 User Manual for the daily rinsing and
cleaning of the system.
1.3. Weekly customer maintenance

An overall check for cleanliness of the system is recommended every
week.
All traces of blood or reagent have to be wiped off as soon as possible
using a piece of cloth and distilled water.
CAUTION
Never use solvent or abrasive cleaning material to clean the system.
page 2/12
8. Maintenance
RAA 016 B Ind A
1.4. Maintenance table

The ABX Pentra 60 maintenance is scheduled on 3 different maintenance kits. The maintenance schedule depends on the number of
cycles per day, follow the chart table to know the kit part number to
use:
CYCLES PER DAY
<30
MAINTENANCE EACH 1Y
Sampling pr o be O'r ing
30 to 120
>120
2Y 6M
1Y 18M 2Y 6M
1Y
PART NUMBER
KIT TO USE
XEA 485 AS
Reagent syr inge O'r ing
Dr aining and Co unting syr inges

O'r ing
Sampling syr inge
5DIFF syr inge
Dr aining chamber
Optical bench lamp
LMNE flo wcell co axial
Diluent r eser vo ir jo int
Co unting heads
Sampling pr o be r eplacement
Pr o cedur e:
RAS 236 A
XEA 486 AS
Pr o cedur e:
RAS 237 A
X
XEA 581 AS
Reagent pisto ns r eplacement
Co unting and Dr aining syr inge

pisto ns r eplacement
Pr o cedur e:
RAS 238 A
page 3/12
8. Maintenance
2. MAINTENANCE
RAA 016 B Ind A
KITS
2.1. 6 month Maintenance kit

Follow the RAS 236 A procedure for maintenance with the XEA 485
AS kit. The kit is including:
Par t Number
page 4/12
SPARE PARTS KIT

XEA 485 AS
Q ty
FAA 053 A
O'r ing 1.40x1.25 Fluo car bo n

Sampling Needle
FAA 065 A
O'r ing 6.30x2.40 Silico n

Reagent Syr inge
XDA 621 A
O'r ing 30.80x3.80 + Wedge

Vacuum/ Waste pump
XDA 622 A

Reagent Syr inge
XEA 019 A
Silico n Gr ease
8. Maintenance
RAA 016 B Ind A
2.2. Yearly Maintenance kit

Par t Number
SPARE PARTS KIT

XEA 486 AS
Q ty
DAJ 007 A
Lamp 20W 9.5V
FAA 040 A

5DIFF Syr inge
FAA 046 A
O'r ing 2.75x1.60 Vito n

Co axial cable
FAA 064 A

Sampling Syr inge
FAA 066 A

Dr aining Chamber
FAA 067 A

5DIFF Syr inge
GBG 156 A
Co unting head EPO Jo int

Aper tur e
XBA 399 A
XEA 286 AS
Waste chamber Jo int + Washer
page 5/12
8. Maintenance
RAA 016 B Ind A
2.3. Pistons Maintenance kit

Par t Number
page 6/12
PISTONS KIT
XEA 581 AS
Q ty
GBC 031 A
Syr inge, Lyse pisto n
GBC 030 A
Syr inge, Reagent pisto n
GBG 052 A
Syr inge, Vacuum/ Waste pisto n
GBG 091 A
Car r iage, Needle guide
XDA 619 AS
Needle, Sampling needle
8. Maintenance
RAA 016 B Ind A
2.4. Fitting Kit

The fitting kit contains the most common pneumatic and hydraulic
parts:
Par t Number
FITTING KIT
XEA 311 A
Q ty
EAA 005 A
Str aight co nnecto r 1.6mm 1/ 8
10
EAA 006 A
Str aight co nnecto r
10
EAA 009 A
Bent co nnecto r I=1.5 10/ 32
10
EAB 002 A
"L" Co nnecto r
10
EAB 003 A
Co nnecto r 4mm H19.5
20
EAB 005 A
"T" Co nnecto r 1.6mm T10-6
30
EAB 006 A
"T" Co nnecto r 2.3mm
30
EAB 009 A
Str aight co nnecto r 1.6mm
30
EAB 010 A
30
EAA 013 A
Bent co nnecto r 1/ 8
10
EAA 014 A
Str aight co nnecto r 1/ 8
10
EAB 026 A
"Y" Co nnecto r 2.5mm
EAB 021 A
"Y" Co nnecto r 3mm
EAB 035 A
"T" Co nnecto r 2.3mm T220-6
10
EAB 014 A
Str aight co nnecto r "Y" 1.5mm/ 1.5mm
10
page 7/12
8. Maintenance
RAA 016 B Ind A
2.5. Screws Kit

The screws kit contains the most common screws used in Pentra 60:
Par t Number
page 8/12
SCREWS KIT
XEA 293 AS
Q ty
KAA002A
CHC M3x6 Scr ew
20
KAA003A
CHC M3x8 Scr ew
20
KAA005A
CHC M3x12 Scr ew
20
KAA006A
CHC M3x14 Scr ew
20
KAA009A
CHC M3x20 Scr ew
20
KAA011A
CHC M3x30 Scr ew
20
KAA015A
CHC M4x8 Scr ew
20
KAA016A
CHC M4x12 Scr ew
20
KAA017A
CHC M4x16 Scr ew
20
KAA021A
CHC M5x10 Scr ew
20
KAA028A
CHC M4x20 Scr ew
20
KAB002A
FHC M3x6 Scr ew
20
KAB003A
FHC M3x8 Scr ew
KAB004A
FHC M3x10 Scr ew
20
KAB005A
FHC M3x12 Scr ew
20
KAC002A
TC M3x5 Scr ew
20
KAE003A
BHC M4x12 Scr ew
20
KAH001A
HU M3 Nut
20
KAH002A
HU M4 Nut
20
KAH003A
HU M5 Nut
20
KAH024A
Nut 1/ 4-28 (US Std)
10
8. Maintenance
RAA 016 B Ind A
KAJ001A
M Washer Diam.3
20
KAJ002A
M Washer Diam.4
20
KAJ003A
M Washer Diam.5
20
KAJ007A
AZ Washer Diam.3
20
KAJ008A
AZ Washer Diam.4
20
KAJ009A
AZ Washer Diam.5
20
KAJ010A
W Gr o wer Washer Diam.5
20
KAM002A Plastic Cr o sspiece M3x6 Lg.8
10
KAM003A Plastic Cr o sspiece M4x6 Lg.8
10
KAM004A Plastic Cr o sspiece M3.5x6 Lg.6
10
10
10
KAM010A
Plastic Cr o sspiece M3x6 Lg.10
10
KAM011A
Plastic Cr o sspiece M3.5x6

Lg.5.7
10
KAM013A
Plastic Cr o sspiece M3.5x6 Lg.10
10
KAM017A Br ass Cr o sspiece M3.5x6 Lg.3
10
KAB016A
FX M3x8 Scr ew
page 9/12
8. Maintenance
RAA 016 B Ind A
3. PROCEDURES
NOTE
Maintenance and adjustments that need to be done on ABX PENTRA 60 are

divided into procedures according to concerned assemblies. This should
make any update easier as all interventions can be done with the corresponding
procedure on its own.
CONCERNING PARTS:
- Hydraulic maintenance and adjustments.
- Pneumatic maintenance and adjustments.
- Electrical maintenance and adjustments.
- Power supply maintenance and adjustments.
- Electronic maintenance and adjustments.
- Printer maintenance and setup.
NOTE
Each procedure has to be read entirely before beginning the intervention:

RED Procedures are Adjustment procedure
BLEU Procedures are Maintenance procedure
GREEN Procedures are Dismantling procedure
WARNING !
When cleaning instruments, disposable gloves should be worn.
page 10/12
8. Maintenance
RAA 016 B Ind A
3.1. Procedure chart tables

P/N
PROCEDURE
CONCERNS
RAS 235 A
INSTALLATION
Unpacking - Working conditions - General description - Printer &

instrument connection - Reagent connections - Priming & Startup
RAS 236 A
6 MONTH MAINTENANCE
Sampling Probe: O'ring Replacement - Reagents syringe: O'rings

Replacement - Vacuum/Waste pump: O'rings Replacement
RAS 237 A
Y EARLY MAINTENANCE
Sampling syringe: O'ring Replacement - 5DIFF syringe: O'ring

Replacement - Draining chamber: O'ring Replacement - Optical
Bench: Lamp & LM NE f low cell coaxial Replacement - Diluent
r eser voir : Joint and w asher Replacement - Count ing Heads:
RBC/WBC Coaxial, O'ring and Aperture joint Replacement
RAS 238 A
EVERY 2 Y EARS MAINTENANCE KIT
S a m p l i ng p r o b e : P r o b e & G ui d e R e p l a c e m e nt - R e a g e nt s
syr inge: Pist ons Replacement - Vacuum/ Wast e pump: Pist on
Replacement
RAS 239 A
DECONTAMINATION & RINSE
Instrument Decontamination & Rinse
RAS 240 A
LMNE FLOWCELL ADJUSTMENT
Optical Bench Check and Adjustment
RAS 241 A
CHAMBERS ADJUSTMENT
Alignment Check and Adjustment of chambers assembly
RAS 242 A
PROBE ADJUSTMENT
Pr obe Home posit ion Check and Adjust ment - Pr obe posit ion
Check and Adjustment
MAIN BOARD ADJUSTMENT
HGB Blank Adjustment - Aperture Current Check - RBC/PLT Gain

A d j us t m e nt - W B C / B A S O G a i n A d j us t m e nt - D r a i n s e ns o r
A d j u s t m e n t - L M NE s e n s o r A d j u s t m e n t - M o t o r C u r r e n t
Adjustment - Thresholds Adjustment
RAS 244 A
THERMIC ADJUSTMENT
Reagent heating system temperature Check - Reagent heating

syst em t emper at ur e Adjust ment - Ther most at edcompar t ment
t emperat ure Check - Thermost at ed compart ment t emperat ure
Adjustment
RAS 245 A
VACUUM ADJUSTMENT
Draining syringe vacuum Check - Counting syringe vacuum Check

- Counting syringe vacuum Adjustment
RAS 247 A
FRONT PANEL DISMANTLING
Front panel Dismantling - Front panel Mounting
RAS 248 A
HEATER ASSY REPLACEMENT
Heater Assy Dismantling - Heater Assy Mounting
RAS 249 A
BUBBLING ADJUSTMENT
Bubbling Adjustment Menu presentation
RAS 250 A
POWER SUPPLY REPLACEMENT
Power supply Dismantling & Replacement
RAS 251 A
START CY CLE MICROSWITCH

REPLACEMENT
Start cycle microswitch Dismantling - Start cycle microswitch

Mounting
RAS 252 A
CHECK UP AFTER INTERVENTION
Instrument check up: Repeatability - Calibration
RAS 253 A
STEP BY STEP ANALY SIS CY CLE

CHECK
Cycle start condition - Count and Measurement of the WBC

group - RBC/PLT group count - Filling of Tank - Rinsing of the
RBC/PLT count chamber
RAS 254 A
OPTICAL BENCH DISMANTLING &

REPLCEMENT
Optical bench Dismantling - Optical bench Replacement Emission gun location Control - Optical bench lamp alignement
Control
RAS 255 A
BARCODE READER
CONFIGURATION
Instrument configuration for Barcode Reader
RAS 243 A
page 11/12
8. Maintenance
RAA 016 B Ind A
3.2. Required tools and products

T O O L S
DESIGNATION
PART NUMBER
P R O D U C T S
DESIGNATION
EMPTY SAMPLE
TUBES
HEXAGONAL KEYS
DYNAMOMETRIC
SCREW DRIVER
A302
MAG 019 A
SILICONE GREASE
DYNAMOMETRIC
SCREW DRIVER
A301
MAG 020 A
GREASE FOR
MECHANICAL
ASSEMBLIES
DYNAMOMETRIC
SCREW DRIVER
A300
MAG 013 A
SOFT TISSUE
CLAMPS
LIQUID SOAP
SCALPEL
DISTILLED WATER
CUTTING PLIERS
MICROPIPETTE TIP
PAIR OF SCISORS
FLAT PIECE OF
STIFF PLASTIC
VOLTMETER
SYRINGE 5ML
FLAT
SCREWDRIVER
LATEX RBC
BARFLEX
FELT PEN
THERMOMETER
TORX KEYS
page 12/12
PART NUMBER
LAM 004 A
XEA 381 AS
LAD 002 AS
P E N T R A 6 0
INSTALLATION
CONCERNS
1
2
3
4
5
6
7
- Working conditions
- Unpacking
- General description
- Installation
- LX300 Printer connection
- Reagent connections
- Priming and startup
REQUIRED TOOLS
Hexagonal keys
REQUIRED PRODUCTS
PENTRA 60 Reagents:
Bottles,
Waste container.
INTERVENTION TIME
60 minutes
FREQUENCY
SPECIFIC KIT OR CONSUMABLES

Installation kit : XEA 484 A
RAS 235 A Ind.A
08/02/99
INSTALLATION
INSTALLATION KIT
XEA 484 A
INSTALLATION KIT
PART NUMBER LIST
P E N T R A 6 0
Par t Number
Page 2/11
Q ty
DAJ 007 A
Lamp 20W 9.5V
DBH 001 A
Tyr ap LA=2.4 L=92
DBH 002 A
Tyr ap LA=3.6 L=140
DBK 003 A
Adhesive ho lder 3mm
EAE 007 A
Tygo n tube 1.52mm (0.060'')
EAE 006 A
Tygo n tube 1.295mm (0.051'')
EAE 034 A
Tygo n tube 2.54mm (0.100'')
EAE 005 A
Tygo n tube 1.02mm (0.040'')
EAE 008 A
Tygo n tube 2.05mm (0.081'')
EAE 011 A
Cr istal tube 3x6
EAE 011 A
Cr istal tube 3x6
EAE 028 A
Cr istal tube 4x6
EAE 028 A
Cr istal tube 4x6
EAC 019 A
Luer male co nnecto r I=3
EAC 010 A
Luer bushing I=3
EAB 021 A
"Y" co nnecto r 3mm
EAB 010 A
EAB 035 A
"T" co nnecto r 2.3mm T220-6
FAA 053 A
FBL 001 A
Rubber cap 2 ho les
GBG 156 A
EPO co unting head Jo int
GBG 144 A
Reagent str aw cap 27
GBG 145 A
Reagent str aw cap 20
GAK 302 A
Facto r y bo ttle cap
GBG 155 A
Cap 25
MAB 018 A
Bent allen key 3mm
MAB 002 A
Bent allen key 2.5mm
MAB 090 A
Bent To r x key T10
XDA 619 AS
Equipped needle
XDA 483 B
Valve 3v (no so leno id)
XDA 621 A
XEA 018 A
Diluent str aw lg. 360mm
XEA 019 A
Gr ease KM 1011
FBH 016 A
Instr ument co ver
RAS 235 A Ind.A
INSTALLATION
1 - Environment
The ABX PENTRA 60 should be operated in an indoor location only. Operation at an altitude
over 2000 meters (6000 Feet) is not recommended. Instrument is designed to be safe for
transient voltages according to INSTALLATION CATEGORY II and POLLUTION DEGREE 2.
Please ask your ABX representative service center for any information about operating
location when it does not comply with the recommended specifications.
2 - Location
The ABX PENTRA 60 should be placed on a clean and level table or work station. Please note
that the ABX PENTRA 60, printer and reagents weigh approximately 40 kilograms (88 lbs).
Avoid exposure to sunlight. Proper ventilation requires that a space of at least 20 cm (8
inches) must be left behind the apparatus.
3 - Grounding
Proper grounding is required. Check that the wall ground (earth) plug is correctly connected to
the laboratory grounding electricity installation. If there is no ground then use a ground stake.
Current electricity Standards must be applied.
4 - Humidity and temperature conditions
The ABX PENTRA 60 must function between 16 to 34C (61 to 93F). Maximum relative
humidity 80% for temperatures up to 31C (88F) decreasing linearly to 50% relative humidity
at 40C (104F). If it is kept at a temperature less than 10C (50F), the instrument should be
allowed to sit for an hour at the correct room temperature before use.
5 - Electromagnetic environment check
The ABX PENTRA 60 has been designed to produce less than the required level of
electromagnetic interferences in order to operate in conformity to its destination. The
electromagnetic interferences by the ABX PENTRA 60 are limited to a level allowing the
correct operation of other instruments in conformity with their destination.
In case of problems, check that the instrument is not placed in the proximity of electromagnetic
fields, or short wave emissions (radars, X-rays, scanner, etc...).
6 - Environment protection
When old the instruments and their accessories must be collected by a laboratory specialized
in elimination and recycling of this kind of equipment according to the legislation.
RAS 235 A Ind.A
P E N T R A 6 0
WORKING CONDITIONS
Page 3/11
INSTALLATION
P E N T R A 6 0
GENERAL DESCRIPTION
Page 4/11
1. Cover:
Before any attempt to remove the cover, open the
pneumatic access door.
2. Pneumatic access door:
allows the operator to access hydraulic parts for maintenance operations. It is mandatory to keep the door
locked during the measuring cycles as it ensures
the heating of the dilutions !
3. Sampling probe:
This needle is used for the 53l whole blood sampling.
4. Control panel:
This panel allows the operator to communicate with the
instrument :
- To access the different cycles
- To identify the patients
- To setup the instrument, etc...
5. Door to reagents bottles:
Access to the reagent bottles in order to replace them
when empty.
Diag.1
Turn the locker as shown on the Diag. 2 to open the pneumatic protection door.
Diag.3
Diag.2
1 - Mechanical and Hydraulic modules (Diag.4&5)
1. Sampling carriage:
Ensuring the needle positioning for the different sampling stages and the distribution.
Support the sampling syringe and the distribution of the blood
2. Sampling syringe:
Take the specimen and distribute the portions into the different chambers.
Take the sample from the first dilution and distribute into the RBC/PLT chamber.
3. Count assembly:
Receives the different rinsing and dilutions.
Ensure the temperature control of the dilutions.
Ensure the counts for WBC, BASOS, RBC, PLT, Hct, Hb.
RAS 235 A Ind.A
INSTALLATION
P E N T R A 6 0
Diag.4&5
4. Draining syringe:
Draining of the different chambers.
Bubbling of the mixtures.
Transfer, by vacuum, the LMNE specimen of the mixing chamber towards the injector of the
optical chamber.
5. Diluent tank:
To hold the necessary diluent for an analysis cycle.
To avoid the risk of the degassing of the diluent as it is being aspirated by the syringes.
This tank is vacuum filled by the counting syringe.
6. Counting
Ensure the
Ensure the
Ensure the
syringe:
vacuum for the WBC and the BASOS counts.
vacuum for the RBC and the PLT counts.
vacuum for the filling of the internal tank with diluent.
7. Syringe assembly for the LMNE Matrix:

Ensure the correct proportioning of the stop diluent in the LMNE preparation chamber
Injection of the specimen into the optical chamber
Injection of the interior and exterior gains into the optical chamber.
8. Syringe assembly for the reagent distribution:
Ensure the correct distribution of the different reagents.
Lysing agent for hemoglobin (ABX ALPHALYSE)
Cleaning reagent (ABX CLEANER)
Lysing agent LMNE (ABX EOSINOFIX)
Diluent used for the dilutions (ABX DILUENT)
except the LMNE stop diluent
Lysing agent for WBC/BASO (ABX BASOLYSE ll)
9. Optical bench:
Ensure the support and the adjustment of the optical chamber
Ensure the support and the adjustment of the lamp
Ensure the support and the adjustment of the optical and electronic elements.
RAS 235 A Ind.A
Page 5/11
INSTALLATION
P E N T R A 6 0
2 - Rear panel
Internal left1 hand

side,
- Waste
output
2 - Diluent input
Mother board
3 - Serial number label

4 - Barcode reader connector
5 - Printer connector
6 - RS 232 C Output
7 - Power supply cable
Diag.6
INSTALLATION
- Turn the locker (as shown on the Diag.2) to open the pneumatic protection door.
- Free the probe carriage removing both plastic blockers (Diag.7).
Diag.7
- Connect the power suplly cable (Diag.6 rep.7).
Page 6/11
RAS 235 A Ind.A
INSTALLATION
Use Luer connectors delivered in the Installation kit to connect Reagents.

- Bottle & Container connections:
1 - ABX ALPHALYSE
2 - ABX BASOLYSE II
3 - ABX EOSINOFIX
4 - ABX CLEANER
5 - ABX DILUENT
6 - WASTE CONTAINER
2 - Diluent & Waste containers connection
- Remove the diluent straw.
- Install the straw deep in the container.
IMPORTANT
Diluent Tube:
Use 3x6 cristal tube for diluent connection. Tube length must be under 2 meters
Waste Tube:
Use 4x6 cristal tube for waste connection. Tube length must be under 2 meters
P E N T R A 6 0
1 - Reagent connections
Diag.8
RAS 235 A Ind.A
Page 7/11
INSTALLATION
P E N T R A 6 0
3 - Bottle connection
Page 8/11
- Open reagent bottles and replace the caps by the bottle stoppper and reagent straw
assembly.
- Open the reagent front door.
- Put the reagent botlles in the compartment.
Diag.9
Diag.10
IMPORTANT
After the priming of one or several reagents, close the pneumatic access door (Right
side of the instrument), switch off the PENTRA 60, then re-start it
LX 300 PRINTER CONNECTION

Setting up the printer:
- Remove all the package protections
- Install the paper-feed knob
Diag.11
RAS 235 A Ind.A
INSTALLATION
P E N T R A 6 0
Diag.12&13
Open the ink ribbon access door at the top of the printer and install the ribbon as shown in
Diag 14 & 15:
- Slide the printer head to the middle of the printer.
- Insert the ribbon cartridge into the printer
- Guide the ribbon between the print head and ribbon guide.
- Slide the printer head from side to side to make sure it moves smoothly.
Diag.14
Diag.15
RAS 235 A Ind.A
Page 9/11
INSTALLATION
P E N T R A 6 0
- Install the paper supports (Diag.16) for single sheets paper use only.
Diag.16
- The printer is connected to the ABX PENTRA 60 with the cable delivered with the instrument. Lock the connector in place by tightening the 2 screws on each end of the connector to
the ABX PENTRA 60. Attach the other end of the cable to the printer and lock the printer
connector in place by the means of the 2 clips located on the connector itself (Diag.17).
Diag.17
- For continuous paper, introduce it in the slot at the back of the printer and use the sprocket
covers to load paper, or feeding the paper frontward when using single sheets (see printer
user manual).
Diag.19
Diag.18
Page 10/11
RAS 235 A Ind.A
INSTALLATION
Instrument priming
REAGENTS\LEVEL/CHANGE
16:05
- Open the reagent LEVEL/CHANGE menu.
DILUENT
EOSINOFIX 1 - LEVEL/CHANGE
0%
CHANGE
11%
CHANGE
23%
CHANGE
BASOLYSE II
CLEANER
20%
CHANGE
52%
CHANGE
HGB LYSE
REAGENTS\LEVEL/CHANGE
16:05
- Use the
and
CHANGE key in reverse video.
to display the reagent
DILUENT
EOSINOFIX 1 - LEVEL/CHANGE
100%
CHANGE
99%
CHANGE
98%
CHANGE
BASOLYSE II
- Press
: a prime cycle is automatically run.
- The reagent level is updated to 100%
CLEANER
98%
CHANGE
98%
CHANGE
ALPHALYSE
Never pour reagent from one container to another. Particles present in the bottom of the old
container can contaminate the new reagent, causing unacceptable background counts, especially
for PLTs.
IMPORTANT
Before analyzing samples, visually inspect reagent lines and pumps for air bubbles.
Repeat priming if air bubbles are still present. Call the ABX representative service
department if priming does not eliminate air bubbles.
Never initiate two Lyse prime cycles back-to-back. This causes excessive foaming in
the waste chamber. Run a blank cycle between each Lyse prime cycle.
- Run a STARTUP cycle and follows the ABX PENTRA 60 User Manual's instructions.
RAS 235 A Ind.A
P E N T R A 6 0
PRIMING & STARTUP
Page 11/11
P E N T R A 6 0
6 MONTH MAINTENANCE
CONCERNS
Instrument maintenance step by step
REQUIRED TOOLS
Hexagonal keys
Dynamometric screw driver A302, A301, A300
Cutting pliers
Flat screw driver
Philips screw driver
Torx keys
REQUIRED PRODUCTS
Minoclair
INTERVENTION TIME
2h
FREQUENCY
See Maintenance table

Spare parts kit : XEA 485 AS
RAS 236 A Ind.A
08/02/99
P E N T R A 6 0
6 MONTH MAINTENANCE
Page 2/10
SAMPLING PROBE
REAGENTS SYRINGE
DRAINING & COUNTING SYRINGES
THERMOSTATED COMPARTMENT CLEANING
6 MONTH MAINTENANCE KIT

Par t Number
SPARE PARTS KIT

XEA 485 AS
Q ty
FAA 053 A

Sampling Needle
FAA 065 A

Reagent Syr inge
XDA 621 A

Vacuum/ Waste pump
XDA 622 A

Reagent Syr inge
XEA 019 A
Silico n Gr ease
TIGHTENING TORQUE TABLE

REP
(DIAG n)
SCREWS
DESIGNATION
TIGHTENING
TORQUES
mN.m (Ozf.in)
1 (1)
2x CHC M3x25
100 (14.2)
(6)
9x CHC M3x12
700 (99.4)
(6)
2x FX M3x12
400 (56.8 )
NOTE
RAS 236 A Ind.A
6 MONTH MAINTENANCE
1 - Oring replacement (1xFAA 053 A)

Switch on the instrument
Run Menu: Maintenance\Technician\Others\Maintenance carriage position
Open the pneumatic access door (Right side of the instrument)
Unscrew the 2 fixation screws (2x CHC M3x25) of the Probe rinsing block on the
carriage (Diag.1)
Diag.1
Diag.2
Take out the Probe and the guide at the same time (lift the Probe locker to free the
Probe) (Diag.2 & 3)
Put the Probe back to its support
Diag.4
Diag.3
Open rinsing block assembly and replace the old Oring joint by the new one in the 6
month maintenance kit (use one drop of silicon grease between 2 fingers for Oring)
(Diag.4)
Reassemble following previous steps backward
When startup is done check that there is no leak
P E N T R A 6 0
SAMPLING PROBE
TORQUE
Rinsing block screws:

100mN.m (14.2 Ozf.in)
RAS 236 A Ind.A
Page 3/10
6 MONTH MAINTENANCE
P E N T R A 6 0
REAGENTS SYRINGE
Page 4/10
1 - Oring replacement (1xFAA 065 A & 4xXDA 622 A)

Switch off the instrument and disconnect power supply cable
Open the left side panel and the mother board door (lock it)
WARNING !
A flat cable is connected at the back of the mother board, becareful when you open
the door that you dont disconnect or damage it
Disconnect the tubes (Diag.5):
Valve 6 inlet 3,
Valve 7 inlet 3,
Valve 8 inlet 3,
Valve 9 inlet 3,
Valve 11 inlet 3
Unscrew the 2 fixation screws of the reagents syringe (Diag.5)
Diag.5
Retire gently the reagents syringe
Drain syringe pushing Pistons in a waste container
WARNING !
A washer is adapted to each Oring joint tore diameter, so that it is mandatory to keep
Oring joint and its washer together
Lyse piston Oring had no washer
On a piece of absorbant paper open the reagents syringe removing the bottom plate
(9x CHC M3x12, 2x FX M3x12)
Replace Oring joints by new one in the 6 month maintenance kit (use one drop of
silicon grease between 2 fingers for Oring) (Diag.6)
RAS 236 A Ind.A
6 MONTH MAINTENANCE
9x CHC M3x12:
700mN.m (99.4 Ozf.in)
2x FX M3x12:
400mN.m (56.8 Ozf.in)
Diag.6
When startup and prime are done check that there is no leak
RAS 236 A Ind.A
P E N T R A 6 0
TORQUES
Page 5/10
6 MONTH MAINTENANCE
P E N T R A 6 0
DRAINING & COUNTING SYRINGES
Page 6/10
1 - Oring replacement on Draining Syringe (1xXDA 621 A)

Switch off the instrument
Disconnect power supply cable
Open the back side panel (6x CHC M3xXX) (Diag.7)
Diag.7
Disconnect the electronic connectors behind the instrument (Diag.8)
Diag.8
Cut the tyraps (Diag.9)
Disconnect grounding wire on the frame (Diag.9)
Put a piece of aborbant paper under the syringe and disconnect both tube
RAS 236 A Ind.A
Diag.9
6 MONTH MAINTENANCE
Diag.10
Unscrew the 4 CHC M4x16 syringe screws (just few turns to free silent block) (Diag.10)
Remove the syringe
Drain the syringe pushing the piston nut up and down several times
Open the syringe (4x CHC M4x16 screws) (Diag.11)
Diag.12
Replace the old Oring joint and washer by the new one in the 6 month maintenance kit
(use one drop of silicon grease between 2 fingers for Oring and piston) (Diag.12)
RAS 236 A Ind.A
P E N T R A 6 0
Diag.11
Page 7/10
P E N T R A 6 0
6 MONTH MAINTENANCE
2 - Oring replacement on Counting Syringe (1xXDA 621 A)
WARNING !
Open the back side panel
Unscrew the 4 CHC M4x16 syringe screws (just few turns to free silent block) (Diag.14)
Diag.13

Put a piece of absorbant paper under the syringe and disconnect all the tubes from the
syringe (Diag.15)
Diag.16
Diag.15
Remove the syringe
Open the syringe (4x CHC M4x16 screws) (Diag.16)
Page 8/10
Diag.14
RAS 236 A Ind.A
6 MONTH MAINTENANCE
Replace the old Oring joint and washer by the new one in the 6 month maintenance kit
(use one drop of silicon grease between 2 fingers for Oring and piston) (Diag.17)
RAS 236 A Ind.A
P E N T R A 6 0
Diag.17
Page 9/10
6 MONTH MAINTENANCE
P E N T R A 6 0
THERMOSTATED COMPARTMENT CLEANING
Page 10/10
1 - Cleaning
Concerned parts:
- Black plastic covering chambers (on the door)
- White plastic under chambers
- Top of the hemoglobin chamber
Put Minoclair on soft paper and sweep all the concerned parts
RAS 236 A Ind.A
P E N T R A 6 0
YEARLY MAINTENANCE
CONCERNS
08/02/99
REQUIRED TOOLS
Hexagonal keys
Clamps
Scalpel
Cutting pliers
Flat screw driver

Torx keys
REQUIRED PRODUCTS
Empty sample tubes
Silicone grease
Soft tissue
Liquid soap
Distilled water
Micropipette tip
RBC/PLT Latex
INTERVENTION TIME
2 h 30
FREQUENCY
The yearly maintenance frequencies vary with the instrument output.
According to the number of cycle, 3 categories are created :
0 - 30 cycles/day -> 1 overall maintenances/year
30 - 120 cycles/day -> 2 overall maintenances/year
> 120 cycles/day -> 3 overall maintenances/year

RAS 237 A Ind.A
P E N T R A 6 0
YEARLY MAINTENANCE
Page 2/13
SAMPLING SYRINGE
5DIFF SYRINGE
OPTICAL BENCH LAMP
LMNE FLOWCELL COAXIAL
DILUENT RESERVOIR JOINT
DRAINING CHAMBER
COUNTING HEADS
YEARLY MAINTENANCE KIT

Par t Number
SPARE PARTS KIT

XEA 486 AS
Q ty
DAJ 007 A
Lamp 20W 9.5V
FAA 040 A

5DIFF Syr inge
FAA 046 A
O'r ing 2.75x1.60 Vito n

Co axial cable
FAA 064 A

Sampling Syr inge
FAA 066 A

Dr aining Chamber
FAA 067 A

5DIFF Syr inge
GBG 156 A
Co unting head EPO Jo int

Aper tur e
XBA 399 A
XEA 286 AS
Waste chamber Jo int + Washer

REP
(DIAG n)
SCREWS
DESIGNATION
TIGHTENING
TORQUES
mN.m (Ozf.in)
1 (2)
2x CHC M4x30
100 (14.2)
2 (4)
3x FX M3x12
400 (56.8)
3 (6)
2x CHC M4x30
100 (14.2)
4 (7)
2x FX M3x12
400 (56.8)
5 (7)
4x CHC M3x12
700 (99.4)
6 (21)
4x CHC M3x20
120 (17)
7 (23)
4x CHC M4x12
100 (14.2)
8 (24)
2x CHC MXxXX
100 (14.2)
9 (26)
2x CHC MXxXX
100 (14.2)
RAS 237 A Ind.A
YEARLY MAINTENANCE
1 - Oring replacement (2xFAA 064 A)

Disconnect the diluent tube between valve 18 and sampling syringe (Diag.1) The diluent is drained through the sampling syringe
Unscrew both fixation screws of the Sampling syringe on the carriage (Diag.2)
Diag.2
Diag.1
Disconnect from the syringe output tube (Diag.3)
Unscrew the 3 FX M3x12 screws from the bottom of the syringe (Diag.4)
Diag.3
Remove the bottom plate (3x FX M3x12), the piston
axle and the 2 Oring joints
Replace both Oring joints by new one in the yearly
maintenance kit (use one drop of silicon grease
between 2 fingers for both Oring)
When startup and prime are done check that there
is no leak
RAS 237 A Ind.A
P E N T R A 6 0
SAMPLING SYRINGE
Diag.4
TORQUE
3x FX M3x12:
400mN.m (14.2 Ozf.in)
Page 3/13
YEARLY MAINTENANCE
P E N T R A 6 0
5DIFF SYRINGE
1 - Oring replacement (1xFAA 040 A & 4xFAA 067 A)
WARNING !

Valve 2 inlet 2,
Valve 3 inlet 3,
Valve 5 inlet 1
Unscrew both fixation screws of the 5DIFF syringe (Diag.6)
Disconnect gently from the syringe the 2 left tubes
Diag.5
Diag.6
Remove the bottom plate (2x FX M3x12, 4x CHC M3x12), the piston axles and the
Oring joints (Diag.7)
Diag.7
Page 4/13
RAS 237 A Ind.A
Diag.8
YEARLY MAINTENANCE
TORQUES
4x CHC M3x12:
700mN.m (99.4 Ozf.in)
2x FX M3x12:
400mN.m (56.8 Ozf.in)
RAS 237 A Ind.A
P E N T R A 6 0
Replace all Oring joints by new one in the yearly maintenance kit (use one drop of
silicon grease between 2 fingers for each Oring and piston) (Diag.8)
Page 5/13
YEARLY MAINTENANCE
P E N T R A 6 0
OPTICAL BENCH LAMP
Page 6/13
1 - Lamp replacement (1xDAJ 007 A)

Open the cover
Disconnect the lamp supply (Diag.10)
Unscrew lamp fixation screws (few turns) (Diag.9)
Diag.9
Diag.10
Turn the lamp and remove it (Diag.10)

Change the lamp
Put back the fixation system and block the screws
Reconnect the lamp supply
RAS 237 A Ind.A
YEARLY MAINTENANCE
1 - Coaxial replacement (1xXBA 399 A)

Open the cover
Open the left side panel and the mother board door (block it)
WARNING !
Open the optical bench cover (4 screws Diag.11)
Diag.11
Disconnect the T connector (few diluent drops will leak) (Diag.12&13)
Diag.12
Diag.13
RAS 237 A Ind.A
P E N T R A 6 0
LMNE FLOWCELL COAXIAL
Page 7/13
YEARLY MAINTENANCE
P E N T R A 6 0
To access ground fitting under the flowcell, unscrew the 2 fixation srews (Diag.14)
Page 8/13
Diag.14
Turn slowly the flowcell (take care to the washers)
Unscrew the ground screw using a small philips screwdriver (Diag.15)
Diag.15
Put the new LMNE coaxial instead
Reassemble following previous steps backward (except optical bench cover)
Run Menu: Maintenance\Hydraulics systems\Rinse\Cytometer to get rid of the air
bubbles stuck to the inner optical surfaces
Check that there is no leak and that flowcell contains no or just a very few air bubbles
Put back the optical bench cover
RAS 237 A Ind.A
YEARLY MAINTENANCE
MEASUREMENT\LMNE ADJUSTMENT
LMNE LAMP
6.00
4.00
8.00
TRANSFERT 1TIME
- LEVEL/CGE
198
100
RESISTIVE CHANNEL
300
51
5
ABSORBANCE CHANNEL
70
180
10
240
Diag.16
Check that the displayed values are within the range
Target values:
TARGET
VALUE
RANGE
LMNE LAMP
6.00
5.50 to 6.50
TRANSFERT TIME
200
150 to 250
RESISTIVE CHANNEL
50
45 to 55
ABSORBANCE CHANNEL
180
180+10
PARAM ETER
Diag.17
If you are out of range perform a RAS 240 LMNE Flowcell adjustment procedure
RAS 237 A Ind.A
P E N T R A 6 0
2 - LMNE Flowcell adjustment check

Run Menu: Maintenance\Technician\Measurement\LMNE Adjustment
Mix RBC/PLT Latex thoroughly and press ESC to start sampling
When optical bench is well adjusted:
Page 9/13
YEARLY MAINTENANCE
P E N T R A 6 0
DILUENT RESERVOIR JOINT

1 - Joint and Washer replacement (1xXEA 286 AS)
Run Menu: Maintenance\Hydraulics systems\Drain chamber\Diluent reservoir
When cycle is finished disconnect the drain tube under the reservoir
Unscrew the 2 fixation screws under diluent reservoir (few turns to release diluent
reservoir support) (Diag.18)
Diag.18
Lift reservoir, take care that no tube is diconnected from reservoir
Unscrew the 4 reservoir screws on top
Replace joint and washer by new one in the yearly maintenance kit (use one drop of
silicon grease between 2 fingers)
Locate new washer and joint against the reservoir top, then the diluent reservoir body
(Diag.19)
NOTE
Locate reservoir body to have inferior support turned back and tubes in the upper part
in front
TORQUE
4x CHC M3x20:
120mN.m (56.8 Ozf.in)
Diag.19
Screw the 4 reservoir screws on top
Put back diluent reservoir in its location and block screws
Connect drain tube under diluent reservoir
Page 10/13
RAS 237 A Ind.A
YEARLY MAINTENANCE
1 - Oring replacement (FAA 066 A)

Run Menu: Maintenance\Hydraulics systems\Drain chamber\All chambers
Turn gently the chambers bottom between 2 fingers (Diag.20)
Diag.21
Diag.20
Replace the Oring joint (Diag.21)
Diag.22
Repeat with the other chambers (Diag.22)
RAS 237 A Ind.A
P E N T R A 6 0
DRAINING CHAMBER
Page 11/13
YEARLY MAINTENANCE
P E N T R A 6 0
COUNTING HEADS
1 - RBC/WBC Coaxial cable Oring replacement (2xFAA 046 A)
Run Menu: Maintenance\Hydraulics systems\Drain chamber\All chambers
Put a piece of white paper or cloth under the Counting chamber assembly
Record the tube positions before dismantling the Counting chamber assembly
Disconnect the chamber tubes (not the wastes)
Dismantle the Counting chamber assembly, 3 screws (Diag.23)
TORQUE
3x CHC M4x12:
100mN.m (14.2 Ozf.in)
Diag.23
Dismantle the electrode loosening the 2 fixation screws (Diag.24)
Diag.24
Diag.25
Use a previously cut micropipette tip to replace the electrode Oring joint as shown in
diagram 25
Reassemble electrode
Repeat with the other electrode (Diag.26)
TORQUE
2x CHC MXxXX:
100mN.m (14.2 Ozf.in)
Page 12/13
RAS 237 A Ind.A
YEARLY MAINTENANCE
Diag.26
TORQUE
2x CHC MXxXX:
100mN.m (14.2 Ozf.in)
Diag.27
CAUTION
Do not manipulate the aperture using hard instruments. Clean the aperture with
a piece of soft paper or preferably, in between 2 fingers.
Rinse thoroughly with distilled water

Dry the exterior of the chamber with a soft paper
CAUTION
Do not apply too much pressure on the electrode fixation screws, as it can
break the aperture (tightening torque = 100mN.m / 14.2 Ozf.in).
It is recommended to reconnect the tubes on the counting head before
reassembling the "electrode/chamber/counting head" assy in order to avoid
applying constraint on the chamber.
Repeat operations for the other counting head

Reassemble electrode
Repeat with the other electrode (Diag.26)
RAS 237 A Ind.A
P E N T R A 6 0
2 - Counting head aperture joint replacement (2xGBG 156 A)

Carefully dismantle the counting head (Diag.26) and plunge the aperture in distilled
water
Replace both EPO joints by new one (Diag.27)
Clean the chamber and the counting head with liquid soap, do not introduce any sharp
instrument inside so as to avoid damaging the inside of the chamber and the aperture
Page 13/13
P E N T R A 6 0
EVERY 2 YEARS MAINTENANCE

CONCERNS
08/02/99
Note: This maintenance and 6 month maintenance has to be performed

at the same time
REQUIRED TOOLS
Hexagonal keys
Clamps
Scalpel
Cutting pliers
Flat screw driver

Torx keys
REQUIRED PRODUCTS
INTERVENTION TIME
2 hours
FREQUENCY
0 to 120 cycles per day: Every 2 years
>120 cycles per day: Every year

RAS 238 A Ind.A
P E N T R A 6 0
Page 2/11
SAMPLING PROBE
REAGENT SYRINGE
COUNTING/DRAINING SYRINGE, PISTON
EVERY 2 YEARS MAINTENANCE KIT

Par t Number
PISTONS KIT
XEA 581 AS
Q ty
GBC 031 A
Syr inge, Lyse pisto n
GBC 030 A
Syr inge, Reagent pisto n
GBG 052 A
Syr inge, Vacuum/ Waste pisto n
GBG 091 A
Car r iage, Needle guide
XDA 619 AS
Needle, Sampling needle

REP
(DIAG n)
SCREWS
DESIGNATION
TIGHTENING
TORQUES
mN.m (Ozf.in)
1 (1)
2x CHC M3x25
100 (14.2)
(6)
9x CHC M3x12
700 (99.4)
(6)
2x FX M3x12
400 (56.8 )
RAS 238 A Ind.B
1 - Probe and Probe Rinsing Block Replacement (1xXDA 621 AS & 1xGBG 091 A)
Unscrew both fixation screws of the Probe rinsing block on the carriage (Diag.1)
Diag.1
Diag.2
Take out the Probe and the rinsing block at the same time (lift the Probe locker to free
the Probe) (Diag.2&3)
Open guide assembly and replace the probe rinsing block and the probe (Diag.4)
Diag.4
Diag.3
WARNING !
Use a new FAA 053 A Oring joint when performing 6 Month maintenance at the same
time
When startup is done check for that there is no leak
RAS 238 A Ind.B
P E N T R A 6 0
SAMPLING PROBE
TORQUE
Rinsing block screws:

100mN.m (14.2 Ozf.in)
Page 3/11
P E N T R A 6 0
REAGENTS SYRINGE
Page 4/11
1 - Pistons replacement (1xFAA 065 A & 4xXDA 622 A)

Switch off the instrument and disconnect power supply cable
Open the left side panel and the mother board door (block it)
WARNING !

Valve 6 inlet 3,
Valve 7 inlet 3,
Valve 8 inlet 3,
Valve 9 inlet 3,
Valve 11 inlet 3
Unscrew both fixation screws of the reagents syringe (Diag.5)
Diag.5
Retire gently the reagents syringe
WARNING !
A washer is adapted to each Oring joint tore diameter, so that it is mandatory to keep
Oring joint and its washer together
RAS 238 A Ind.B
TORQUES
9x CHC M3x12:
400mN.m (99.4 Ozf.in)
2x FX M3x12:
400mN.m (56.8 Ozf.in)
Diag.6
Replace Pistons by new ones in the every 2 years maintenance kit (use one drop of
silicon grease between 2 fingers for each piston) (Diag.6)
WARNING !
Be sure to use the new washers corresponding to the new Oring joints, when
performing 6 Month maintenance at the same time

When startup and prime are done check for that there is no leak
RAS 238 A Ind.B
P E N T R A 6 0
Drain syringe pushing Pistons in the waste container

On a piece of absorbant paper open the reagents syringe removing the bottom plate
(9x CHC M3x12, 2 FX M3x12)
Page 5/11
P E N T R A 6 0
COUNTING/DRAINING SYRINGE
Page 6/11
1 - Piston replacement on Vacuum pump (XDA 621 A)

Open the back side panel (Diag.7)
Diag.7
Diag.8
Cut the tyraps (Diag.9)

Put a piece of aborbant paper under the syringe and disconnect both tube
RAS 238 A Ind.B
Diag.9
Unscrew the 4 syringe screws (just few turns to free silent block) (Diag.10)
Remove the syringe
Remove the syringe guiding plate (4 torx screws) (Diag.11)
Diag.11
Open the syringe (4 screws) (Diag.12)
Diag.12
RAS 238 A Ind.B
P E N T R A 6 0
Diag.10
Page 7/11
P E N T R A 6 0
Page 8/11
Diag.13
Diag.14
Remove the 3 pistons screws (CHC M3x16) (Diag.14)
IMPORTANT
Take care to keep syringe nut in place on the motor screw
Replace Piston by new one in the every 2 years maintenance kit (use a bit of silicon
grease)
WARNING !
Be sure to use the new washer corresponding to the new Oring joint, when performing
6 Months maintenance at the same time
RAS 238 A Ind.B
WARNING !
Open the back side panel (Diag.15)
Diag.15

Unscrew the 4 syringe screws (just few turns to free silent block) (Diag.17)
P E N T R A 6 0
2 - Piston replacement on Waste pump (XDA 621 A)

Diag.17
Diag.16
RAS 238 A Ind.B
Page 9/11
P E N T R A 6 0

Put a piece of aborbant paper under the syringe and disconnect all the tubes from the
syringe (Diag.18)
Diag.18
Remove the syringe
Remove the syringe guiding plate (4 torx screws) (Diag.19)
Diag.19
Open the syringe (4 screws) (Diag.20)
Diag.20
Page 10/11
RAS 238 A Ind.B
Diag.22
Remove the 3 pistons screws (CHC M3x16) (Diag.22)
IMPORTANT
Take care to keep syringe nut in place on the motor screw
Replace Piston by new one in the every 2 years maintenance kit (use one drop of
silicon grease between 2 fingers for piston) (Diag.21)
WARNING !
Be sure to use the new washer corresponding to the new Oring joint, when performing
6 Month maintenance at the same time
RAS 238 A Ind.B
P E N T R A 6 0
Diag.21
Page 11/11
P E N T R A 6 0

CONCERNS
Instrument decontamination before maintenance operation in the following
cases:
- Instrument removed from a biohazardous area
- Maintenance intervention on contaminated assemblies
and instrument rinse
REQUIRED TOOLS
Hexagonal keys
Clamps
Flat screw driver
Torx keys
REQUIRED PRODUCTS
Fungicidal, bactericidal, virus killing detergent spray, non corrosive for metals,
Non plastic altering.
Bleach solution 12Cl
Deionize water
Protection gloves
Absorbant paper
Distilled water
INTERVENTION TIME
1h35min
FREQUENCY
On request
RAS 239 A Ind.A
08/02/99
P E N T R A 6 0
DECONTAMINATION
Page 2/4
1 - Preparation (20min)
Run Menu: Maintenance\Hydraulics systems\Concentrated cleaning (Diag.1)
WARNING !
- Disposable gloves and lab coat must be worn by the operator
- Local or national regulations must be applied in at all the times
MAITENANCE\HYDRAULICS SYSTEMS
1234-
16:05
MAINTENANCE\HYDRAULICS SYSTEMS
16:05
1-BACKFLUSH
2-RINSE
3-DRAIN CHAMBERS
4-CONCENTRATED CLEANING
BACKFLUSH
RINSE
DRAIN CHAMBERS
CONCENTRATED CLEANING
CYCLE IN PROGRESS. PLEASE WAIT......

21%
Diag.1
MAINTENANCE\HYDRAULICS SYSTEMS
Diag.2
16:05
POUR2-HYDRAULICS
3 ml OF MINOCLAIR
INTO CHAMBERS
SYSTEMS
PRESS ESC KEY TO CONTINUE
3-MECHANICAL SYSTEMS
CYCLE IN PROGRESS. PLEASE WAIT......

31%
Diag.3
Pour 3ml of Minoclair in each chamber
Press ESC to continue
When cycle is finished, switch off the instrument and remove the supplying cable
Open the instrument cover
Spray the bactericidal cleaner on all biohazardous areas and wait for 10 minutes
(assemblies in contact with the biohazardous material such as instrument cover, tube
holder, keyboard, start key, assemblies close to the SN...
RAS 239 A Ind.A
Concerned assemblies
Outer surfaces of the instrument (perpex, covers, LCD, reagent locations....)
Keyboards
Waste connector plug
Liquid valve push button
Assemblies close to the needle
Tube holder assembly
Overflow trays
Reinstall all the assemblies and setup the instrument back to its initial configuration
3 - Analysis circuit decontamination (30 min)
Prepair 1 bottle containing 1/2 litre of bleach diluted to 1 part of bleach for 9 parts of
deionize water (1/10)
Prepair 1 bottle containing 1/2 litre of distilled water
Replace the reagent bottles by the diluted bleach bottle
Run 6 All reagents prime cycles Menu: Reagents\Prime\All Reagents (Diag.4)
REAGENTS\PRIME
16:05
1- DILUENT
2- CLEANER
3- EOSINOFIX
4- BASOLYSE II
5- ALPHALYSE
6- ALL REAGENTS
Diag.4
Diag.5
Fill a sample tube with diluted bleach to 1 part of bleach for 4 of deionize water (1/5)
Run 15 cycles Burn-in Menu: Technician\User mode\Burn-in leave the instrument
operating until it stops
RAS 239 A Ind.A
P E N T R A 6 0
2 - Manual decontamination (20 min)

Dilute the 12cl bleach to 1 part of bleach for 4 of deionize water (1/5)
Instrument environment must be cleaned and decontaminated
No sponge, nor cloth must be used. Only absorbant paper, thrown after use, in contamination bins, have to be used. For small or sensitive assemblies use accurate drier
papers
All assemblies suspected to have been in contact with biohazardous material must be
disinfected with diluted bleach (the stainless steel must be bleached below 30Celsius)
Blood stains or salt marks must be cleaned with spray detergent first
Page 3/4
P E N T R A 6 0
DECONTAMINATION
Page 4/4
1 - Drain and rinse (30 min)

Remove the 3 reagent straws from the bottle containing the diluted bleach
Wrap the straws in absorbant paper
Run 2 All reagents prime cycles Menu: Reagents\Prime\All Reagents (Diag.4) the
bleach is drained
Replace the diluted bleach by the distilled water bottle and re-plunge the straws in
distilled water
Run 6 All reagents prime cycles Menu: Reagents\Prime\All Reagents (Diag.4) (Rinse)
Remove the 3 reagent straws from the distilled water (Wrap the straws in absorbant
paper)
Run 2 All reagents prime cycles Menu: Reagents\Prime\All Reagents (Diag.4) the
distilled water is drained
Run a STAND BY cycle
Re-install the reagent bottles and the straws
Close the instrument cover
WARNING !
When rinse is finished check that LMNE chamber is empty
RAS 239 A Ind.A
P E N T R A 6 0

CONCERNS
Instrument adjustment:
LMNE Flowcell
REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Torx keys
REQUIRED PRODUCTS
Latex RBC\PLT
Fresh blood samples
Calibrator
INTERVENTION TIME
1h
FREQUENCY
On request

None
RAS 240 A Ind.A
08/02/99
P E N T R A 6 0
LMNE FLOWCELL CHECK & ADJUSTMENT
Page 2/6
NOTE
RAS 240 A Ind.A
This adjustment procedure must be carried out when LMNE flowcell is moved or replaced,
or when LMNE tube is changed. No adjustment is required when LMNE Lamp is replaced
Menu: Maintenance\Technician\Measurement\LMNE Adjustment allows the
adjustment of the entire optical bench parameters. A 1:400th dilution of the RBC/PLT
Latex is prepared (5l of Latex, 2ml of diluent) in the LMNE chamber. This dilution is then
transfered toward the optical chamber and injected
The measurments are continuously displayed every 700ms during a total of 27 seconds
WARNING !
Never dismantle Emission gun or even unlock it. The adjustment is factory made and
cannot be performed in the field.
1 - Preparation
Open the cover
Open the left side panel and the main board door (block it)
WARNING !
Diag.1
Run Menu: Maintenance\Hydraulics systems\Rinse\Cytometer to get rid of air
bubbles stuck to the inner optical surfaces
Check that the flowcell contains no or just a very few air bubbles
RAS 240 A Ind.A
P E N T R A 6 0
LMNE FLOWCELL CHECK & ADJUSTMENT
Page 3/6
P E N T R A 6 0
Page 4/6
2 - LMNE Flowcell adjustment check

IMPORTANT
Mix RBC/PLT LATEX thoroughly and for few minutes !
Press ESC to start sampling

The following screen is displayed (When optical bench is well adjusted):
MEASUREMENT\LMNE ADJUSTMENT
LMNE LAMP
6.00
4.00
8.00
TRANSFERT 1TIME
- LEVEL/CGE
100
RESISTIVE CHANNEL
198
300
51
5
ABSORBANCE CHANNEL
70
180
10
240
Diag.2
Check that the displayed values are within the range:
TARGET
VALUE
RANGE
LMNE LAMP
6.00
5.50 to 6.50
TRANSFER TIME
200
150 to 250
RESISTIVE CHANNEL
50
45 to 55
>170
Max has to be
reached
PARAM ETER
ABSORBANCE CHANNEL
Diag.3
If it is out of range or to get a better adjustment follow this procedure
RAS 240 A Ind.A
IMPORTANT
Mix LATEX thoroughly and for a few minutes !

Press ESC to start sampling
Adjust LMNE lamp voltage around 6V:
- Adjust by R11 potentiometer located on optical bench board (Diag.4)
NOTE
Test points on Optical bench board, near R11 potentiometer, can be

used to check 6V with a voltmeter.
R11
Diag.4
Adjust Resistive channel to 50:
- Adjustment is made by R136 potentiometer located on mother board (Diag.7
on next page)
Adjust Absorbance channel to the maximum (and above 170):
- Adjustment is made by lateral or axial reception gun movement, use toothed
wheel (Diag.5) and lateral screw (Diag.6)
Diag.5
NOTE
Diag.6
P E N T R A 6 0
3 - LMNE Flowcell position adjustment

If you have difficulties to adjust Absorbance, you can increase gain

with the R148 potentiometer (Diag.7 on next page). Once you get the
highest absorbance adjustment, decrease slowly gain (R148) to get a
correct optical gain adjustment with normal fresh blood.
RAS 240 A Ind.A
Page 5/6
P E N T R A 6 0

Check that Transfer time is in limits of range (around 200):
- Transfer time is not adjustable and must be between 150 and 250
NOTE
R148
R136
Put back the Optical Bench cover
NOTE
Page 6/6
Transfer time bargraph is used to ensure the correct Right/Left location of the light rectangle in the flowcell. When the optimal value is
reached the transfer time is around 200 because of the constant height
between guns alignment and flowcell.
This height is guaranteed by the washers under the flowcell, always
put them back even when the flowcell is replaced by a new one.
Diag.7
After intervention check, with normal fresh blood, that you have a correct matrix.
RAS 240 A Ind.A
P E N T R A 6 0
CHAMBERS ADJUSTMENT
CONCERNS
Chambers assembly adjustment
REQUIRED TOOLS
Hexagonal keys
Flat screw driver
REQUIRED PRODUCTS
INTERVENTION TIME
15min
FREQUENCY

None
RAS 241 A Ind.A
08/02/99
CHAMBERS ADJUSTMENT
P E N T R A 6 0
CHAMBERS ADJUSTMENT
Page 2/5
NOTE
RAS 241 A Ind.A
CHAMBERS ADJUSTMENT
This adjustment procedure must be carried out when chamber assembly is moved or
replaced.
1 - Alignment Check
Open the cover and the right access door
Menu: Maintenance\Technician\Others\Maintenance carriage position
Locate probe as shown in diagrams 1 & 2
Diag.1
Diag.2
Push the carriage backward (without lifting the probe), check that there is the same
height between probe and first and last tops of chamber (Diag.3 & 4)
Diag.3
If you need to get a better adjustment follow this procedure
RAS 241 A Ind.A
Diag.4
P E N T R A 6 0
CHAMBERS ADJUSTMENT
Page 3/5
P E N T R A 6 0
CHAMBERS ADJUSTMENT
Page 4/5
2 - Alignement Adjustment
Free the chamber support by loosening the 4 screws (Diag.5)
Diag.5
Locate probe as shown in diagrams 6 & 7
Diag.6
Diag.7
Tighten gently the left upper screw
RAS 241 A Ind.A
CHAMBERS ADJUSTMENT
Diag.8
Diag.9
If necessary adjust position moving up and down chamber

Perform an alignment check as described at the begenning of this procedure
When adjustment is correct, tighten screws and perform a Probe Adjustment procedure
WARNING !
It is mandatory to perform the Probe adjustment procedure after this adjustment
RAS 241 A Ind.A
P E N T R A 6 0
Push the carriage backward (without lifting the probe), check that there is the same
height between probe and last top of chamber (Diag.8 & 9)
Page 5/5
P E N T R A 6 0
PROBE ADJUSTMENT
CONCERNS
Probe adjustment
REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Torx keys
Lamp
REQUIRED PRODUCTS
None
INTERVENTION TIME
15min
FREQUENCY
On request

None
RAS 242 A Ind.A
08/02/99
PROBE ADJUSTMENT
P E N T R A 6 0
PROBE ADJUSTMENT
Page 2/7
NOTE
RAS 242 A Ind.A
PROBE ADJUSTMENT
IMPORTANT
Check Chambers position before checking or adjusting Probe position. Follow CHAMBERS ADJUSTMENT procedure of this manual
This procedure is to check and adjust probe position in chambers

Menu: Maintenance\Technician\Dilution\ allows the adjustment of carriage and probe
(Diag.1)
Needle Home: Allow adjustment to

get the best probe extremity cleaning
Carriage Home: Default value

is around 20 and doesnt need to
be changed
MAINTENANCE\TECHNICIAN\DILUTION
13:05
NEEDLE HOME
CARRIAGE HOME
28
25
CHECK
NEEDLE POS.
CARRIAGE POS.
476
1136
CHECK
Check (2): Allows to

check that probe is
aligned with one inlet
(LMNE-3)
CHECK CYCLE
Needle pos.: Probe

Up/Down adjustment
Check (1): Allows to

check that probe is out
of 9.4mm from its guide
Diag.1
Carriage pos.: Probe Left/

Right adjustment
Check (3): Allows to check that

probe is aligned with all distribution
inlets
WARNING !
Check Probe position before any adjustment, running Check functions
1 - Needle Home Position Check

Run Menu: Maintenance\Technician\Dilution\Check (1) (Diag.2)
13:05
NEEDLE HOME
CARRIAGE HOME
28
25
CHECK
NEEDLE POS.
CARRIAGE POS.
476
1136
CHECK
P E N T R A 6 0
PROBE ADJUSTMENT
CHECK CYCLE
Diag.2
RAS 242 A Ind.A
Page 3/7
P E N T R A 6 0
PROBE ADJUSTMENT
Check that the probe is about 9.4mm out from the carriage, compare with a 9.4mm
piece of paper that you have previously prepared (Diag.3 & 4)
Diag.3 & 4
Press ESC to continue
2 - Needle Position Check
13:05
NEEDLE HOME
CARRIAGE HOME
28
25
CHECK
NEEDLE POS.
CARRIAGE POS.
476
1136
CHECK
CHECK CYCLE
Diag.5
Disconnect the tube connected to LMNE chamber inlet n3
The right position for your eye is when you see inlet as in Diagram 6 and not as in
diagram 7 where circles are not centered
Diag.6
Diag.7
If the probe is well adjusted you must see it as in the following diagram (Diag.8)
Diag.8
Reconnect the tube to LMNE chamber inlet n3
IMPORTANT
Adjustment must be performed only if required

If you need to get a better adjustment follow the procedure
Page 4/7
RAS 242 A Ind.A
PROBE ADJUSTMENT
13:05
NEEDLE HOME
CARRIAGE HOME
28
25
CHECK
NEEDLE POS.
CARRIAGE POS.
476
1136
CHECK
CHECK CYCLE
Diag.9
Needle home: Allow adjustment to get the best probe extremity cleaning
13:05
NEEDLE HOME
CARRIAGE HOME
28
25
CHECK
NEEDLE POS.
CARRIAGE POS.
476
1136
CHECK
CHECK CYCLE
Diag.10
Check that the probe is about 9.4mm out from the carriage, compare with a 9.4mm
piece of paper that you have previously prepared (Diag.11 & 12)
Diag.11 & 12
If Probe is too low (gap>9.4mm) increase Needle Home value
If Probe is too high (gap<9.4mm) decrease Needle Home value
If necessary adjust Needle Home value and re-run a Check (1) cycle to control gap
RAS 242 A Ind.A
P E N T R A 6 0
3 - Needle Home Position Adjustment

Enter Menu: Maintenance\Technician\Dilution\ (Diag.9)
Page 5/7
P E N T R A 6 0
PROBE ADJUSTMENT
Page 6/7
4 - Needle Position Adjustment

Enter Menu: Maintenance\Technician\Dilution\ (Diag.13)
13:05
NEEDLE HOME
CARRIAGE HOME
28
25
CHECK
NEEDLE POS.
CARRIAGE POS.
476
1136
CHECK
CHECK CYCLE
Diag.13
Needle pos.: Probe Up/Down adjustment

Carriage pos.: Probe Left/Right adjustment
13:05
NEEDLE HOME
CARRIAGE HOME
28
25
CHECK
NEEDLE POS.
CARRIAGE POS.
476
1136
CHECK
CHECK CYCLE
Diag.14
Disconnect the tube connected to LMNE chamber inlet n3
The right position for your eye is when you see inlet as in Diagram 15 and not as in
diagram 16 where circles are not centered
Diag.15
Diag.16
If the probe is well adjusted you must see it as in the following diagram (Diag.17)
Diag.17
RAS 242 A Ind.A
PROBE ADJUSTMENT
If the Probe is too much forward increase Carriage pos. value
Diag.19
If the Probe is too much backward decrease Carriage pos. value
Diag.20
If the Probe is too high increase Needle pos. value
Diag.21
If the Probe is too low decrease Needle pos. value
If necessary adjust Carriage and Probe position
Re-run a Check (2) cycle to control Probe position and restart procedure until you get
the right Probe position
When adjustment is finished check that no tube is disconnected then press ESC
NEEDLE HOME
CARRIAGE HOME
28
25
13:05
CHECK
CHECK : ALL TUBING CONNECTED

CHECK
NEEDLE POS.
476
CARRIAGE POS.
1136
CHECK CYCLE
Diag.22
RAS 242 A Ind.A
P E N T R A 6 0
Diag.18
Page 7/7
P E N T R A 6 0

CONCERNS
Main board adjustment
REQUIRED TOOLS
Small flat screw driver
Hexagonal keys
Voltmeter
REQUIRED PRODUCTS
None
INTERVENTION TIME
30min
FREQUENCY
On request

None
RAS 243 A Ind.A
08/02/99
P E N T R A 6 0
Page 2/10
HB BLANK ADJUSTMENT
APERTURE CURRENT CHECK
RBC/PLT GAIN ADJUSTMENT
WBC/BASO GAIN ADJUSTMENT
DRAIN SENSOR ADJUSTMENT
LMNE SENSOR ADJUSTMENT
MOTOR CURRENT ADJUSTMENT
THRESHOLDS ADJUSTMENT
NOTE
RAS 243 A Ind.A
1 - HGB Blank Adjustment

Open the cover and the left access door to main board
Run Menu: Maintenance\Technician\Measurment\HGB Blank Adjustment (Diag.1)
The utility carries out a rinsing cycle of the WBC chamber and continuously
displays the voltage used by the converter. The adjustment cycle lasts for 20 seconds.
The adjustment is carried out with the R248 potentiometer (Diag.2)
The final adjustment voltage is approximately 4.7 Volts +/- 0V
MEASUREMENT\HGB BLANK ADJUSTMENT
6:45
HGB BLANK ADJUSTMENT

4.77 VOLTS
4.00
5.00
Diag.1
NOTE
This adjustment is made with the right side door closed and under
stabilised thermic conditions (5min at least after startup)
P E N T R A 6 0
HGB BLANK ADJUSTMENT
Diag.2
RAS 243 A Ind.A
Page 3/10
P E N T R A 6 0
APERTURE CURRENT CHECK
Page 4/10
1 - Aperture Current Check

Disconnect LMNE CIS, RBC and WBC coaxes (Diag.3)
Diag.3
Run Menu: Maintenance\Technician\Measurement\Aperture Current (Diag.4)
MAINTENANCE\TECHNICIAN\MEASUREMENT
6:43
CHECK APERTURE CURRENT

PRESS ESCAPE TO CONTINUE
5-LMNE ADJUSTMENT
Diag.4
WARNING !
Do not damage connectors while measuring
IMPORTANT
Use an old coaxe that you have previously cut to control voltages with the
voltmeter
Check Aperture currents in J22, J23 and J24, aperture current measured must be
around 60V (not ajustable)
Press ESC to exit
Reconnect LMNE CIS, RBC and WBC coaxes (Diag.3)
RAS 243 A Ind.A
1 - RBC/PLT Gain Adjustment

A special cycle allows the dilution of the RBC/PLT LATEX to a 1/220th (10 l of LATEX
with 2.2 ml of diluent). A special count programme carries out the calculations of the
mean volume in the predefined zones and displays them every 700 ms. The duration of
the measurement cycle is 21 seconds
The adjustments are made using R133 potentiometer for the RBC and R135 for the
PLT (Diag.7)
Run Menu: Maintenance\Technician\Measurement\RBC/PLT Gain (Diag.5)
Two graphs allow a visual check of the adjustments (Diag.6)
MEASUREMENT\RBC/PLT GAIN
6:45
MEASUREMENT\RBC/PLT GAIN
6:43
RBC
77
56
PRESS ESCAPE TO SAMPLE THE LATEX
96
PLT
111
80
Diag.5
IMPORTANT
Mix RBC/PLT Latex thoroughly before

sampling
135
Diag.6
RBC TARGET VALUE 78

PLT TARGET VALUE 112
P E N T R A 6 0
RBC/PLT GAIN ADJUSTMENT
Diag.7
RAS 243 A Ind.A
Page 5/10
P E N T R A 6 0
WBC/BASO GAIN ADJUSTMENT

1 - WBC/BASO Gain Adjustment
A special cycle allows the dilution of the RBC/PLT LATEX to a 1/733rd (3L of LATEX
with 2.2 ml of diluent). A special count programe carries out the calculations of the mean
volume in the predefined zones and displays them every 700 ms. The duration of the
measurement cycle is 21 seconds
The adjustments are made with the aid of a R134 potentiometer (Diag.10)
Run Menu: Maintenance\Technician\Measurement\WBC/BASO Gain (Diag.8)
A graph allows a visual assessment of the adjustments (Diag.9)
MEASUREMENT\WBC/BASO GAIN
6:43
6:45
MEASUREMENT\WBC/BASO GAIN
WBC/BASO
PRESS ESCAPE TO SAMPLE THE LATEX
101
57
Diag.8
IMPORTANT
Mix Latex thoroughly before sampling
156
Diag.9
GB/BASO
102
TARGET VALUE
Diag.10
Page 6/10
RAS 243 A Ind.A
1 - Drain Sensor Adjustment

Run Menu: Maintenance\Technician\Sensor Check\Draining (Diag.11)
The utility empties the drain cell in order to adjust the voltage to 4.5Volts +/- 0.3 (test
point TP52, R287 potentiometer on the mother card Diag.13)
The utility then fills the sensor with the diluent. check that the voltage on TP52 is less
than 1 Volt (Diag.12)
TECHNICIAN\SENSOR CHECK
11:43
ADJUST SENSOR VOLTAGE

11:43
CHECK SENSOR VOLTAGE
Diag.11
Diag.12
P E N T R A 6 0
DRAIN SENSOR ADJUSTMENT
Diag.13
RAS 243 A Ind.A
Page 7/10
P E N T R A 6 0
LMNE SENSOR ADJUSTMENT

1 - LMNE Sensor Adjustment
Run Menu: Maintenance\Technician\Sensor Check\LMNE Transfer (Diag.14)
The utility empties the drain cell in order to adjust the voltage to 4.5Volts +/- 0.3 (test
point TP48, R286 potentiometer on the mother card Diag.16)
The utility then fills the sensor with the diluent. check that the voltage on TP48 is less
than 1 Volt (Diag.15)
11:43
ADJUST SENSOR VOLTAGE

11:43
CHECK SENSOR VOLTAGE
Diag.14
Diag.15
Diag.16
Page 8/10
RAS 243 A Ind.A
1 - Motor Current Adjustment

Check and adjust, when necessary, all following voltages:
M OTORS
TEST
POINTS
V OLTAGE
POTENTIO.
Draining syringe
TP5
4 V + 0.05 V
R149
Counting syringe
TP6
4 V + 0.05 V
R150
Dilutor syringe
TP7
4 V + 0.05 V
R151
Optical Bench
Injector syringe
TP8
3 V + 0.05 V
R152
Horizontal carriage
TP10
3 V + 0.05 V
R154
Sample syringe
TP11
2 V + 0.05 V
R155
Probe carriage
TP12
5 V + 0.05 V
R156
Potentiometers and Test points are located on Main board as shown on the next diagram:
Draining syringe
Counting syringe
Reagents syringe
Optical Bench
Injector syringe
Horizontal carriage
Sample syringe
Probe carriage
Up/Down
P E N T R A 6 0
MOTOR CURRENT ADJUSTMENT
Diag.17
RAS 243 A Ind.A
Page 9/10
P E N T R A 6 0
THRESHOLDS ADJUSTMENT
Page 10/10
1 - Thresholds Adjustment
Check and adjust, when necessary, all following voltages:
THRESHOLDS
TEST POI NTS
VOLTAGE
POTENTI OM ETERS
BASO
TP14
300 mV + 5
R157
RBC
TP13
300 mV + 5
R158
PLT
TP2
300 mV + 5
R159
LMNE CIS
TP3
650 mV + 5
R160
LMNE OD
TP4
350 mV + 5
R161
Potentiometers and Test points are located on Main board as shown on the next diagram:
Diag.18
PLT
LMNE OD
LMNE CIS
RBC
RAS 243 A Ind.A
BASO
P E N T R A 6 0
THERMIC ADJUSTMENT
CONCERNS
Concerns the enclosed thermic and the preheating of the reagents
REQUIRED TOOLS
Thermometer
REQUIRED PRODUCTS
None
INTERVENTION TIME
30min
FREQUENCY
On request

None
RAS 244 A Ind.A
08/02/99
THERMIC ADJUSTMENT
P E N T R A 6 0
THERMIC CHECKS
Page 2/7
NOTE
RAS 244 A Ind.A
THERMIC ADJUSTMENT
1 - Reagent Heating System Temperature Check

Concerns the checking of the reagent heating system
Open both instrument side to open the cover
Enter Menu: Maintenance\Technician\Heating Systems\Reagent heating system\
(Diag.1)
TECHNICIAN\HEATING SYSTEMS
15:38
1-REAGENT HEATING SYSTEM

2-THERMOSTATED COMPARTMENT
Diag.1
Adjustment: Allows the capture of the CTN value
REAGENT HEATING SYSTEM
15:38
1-ADJUSTMENT
2-REFERENCE
Reference: Allows the modification of the temperature instructions
Diag.2
IMPORTANT
This control must be made with the right side door closed and under stabilised
thermic conditions (5min at least after startup)
Diag.3
Close the right side door
Put the thermometer probe in the LMNE chamber (the one in the middle) (Diag.3)
P E N T R A 6 0
THERMIC CHECKS
IMPORTANT
Make sure to have plunged the thermometer probe in the liquid
RAS 244 A Ind.A
Page 3/7
P E N T R A 6 0
THERMIC ADJUSTMENT
Page 4/7
Select number of cycle to run: 5 (Diag.4)

Run Menu: Maintenance\Technician\Heating Systems\Reagent heating
system\Check (Diag.4)
REFERENCE
REFERENCE TEMPERATURE
15:08
35.0
RUN CYCLE NUMBER 5
CHECK
RUNNING TEMPERATURE LIMITS

MIN 33.0
MAX 37.0
Diag.4
Every time you ear one beep read the Thermometer value
NOTE
To validate check you must get, during control, 10 times the right
temperature
If an adjustment is required, when heating block is replaced, follow this
procedure
RAS 244 A Ind.A
THERMIC ADJUSTMENT
ADJUSTMENT
15:05
CTN VALUE
664
Diag.5
Check and eventualy change the CTN value
NOTE
The reagent heating system CTN value is located on the CTN wire near
the main board (left connector on J31) (Diag.6)
Be sure you read the right value, sticker could be up or down
P E N T R A 6 0
2 - Reagent Heating System Temperature Adjustment

Enter Menu: Maintenance\Technician\Heating Systems\Reagent heating
system\Adjustment (Diag.5)
A LED near the connector shows

the heating command:
- when lit off, heating is off
- when blinking, heating is on
- when lit on, heating is maximum
Diag.6
Make a reagents preheating temperature check (at the begining of this procedure)
IMPORTANT
If you dont get the right temperature, adjust it by the mean of the Reference
temperature (Diag.7)
REFERENCE
15:08
35.0
RUN CYCLE NUMBER 5
CHECK

MIN 33.0
MAX 37.0
Diag.7
When Reference temperature increases, reagent preheating temperature
increases
Make a reagents preheating temperature check (at the begining of this procedure)
If required restart adjustment and check again, until you get an acceptable
temperature
RAS 244 A Ind.A
Page 5/7
P E N T R A 6 0
THERMIC ADJUSTMENT
3 - Thermostated Compartment Temperature Check
Open both instrument side to open the cover
Enter Menu: Maintenance\Technician\Heating Systems\Thermostated
Compartment\ (Diag.8)
TECHNICIAN\HEATING SYSTEMS
15:38
1-ADJUSTMENT
2-REFERENCE
1-REAGENT HEATING SYSTEM

2-THERMOSTATED COMPARTMENT
Diag.9
Diag.8
Adjustment: Allows the capture of the CTN value
Reference: Allows the modification of the temperature instructions
IMPORTANT
Diag.10
Put the thermometer probe in the CTN sensor (Diag.10)
Select number of cycle to run: 5 (Diag.11)
Run Menu: Maintenance\Technician\Heating Systems\Thermostated
Compartment\Check (Diag.11)
REFERENCE
15:08
35.0
RUN CYCLE NUMBER 5
CHECK

MIN 33.0
MAX 37.0
Diag.11
Every time you ear one beep read the Thermometer value
NOTE
Page 6/7
THERMOSTATED COMPARTMENT
15:38
To validate check you must get, during control, 10 times the right
temperature
If an adjustment is required, when heating block is replaced, follow this
procedure
RAS 244 A Ind.A
THERMIC ADJUSTMENT
ADJUSTMENT
15:05
CTN VALUE
664
Diag.12
Check and eventualy change the CTN value, thermostated compartment CTN value
is located on the CTN wire near the main board (right one connected on J33) (Diag.13)
NOTE
The thermostated compartment CTN value is located on the CTN wire

near the main board (right connector on J33) (Diag.13)
Be sure you read the right value, sticker could be up or down
P E N T R A 6 0
4 - Thermostated Compartment Temperature Adjustment

Enter Menu: Maintenance\Technician\Heating Systems\Thermostated
Compartment\Adjustment (Diag.12)
A LED near the connector shows

the heating command:
- when lit off, heating is off
- when blinking, heating is on
- when lit on, heating is maximum
Diag.13
Make a thermostated compartment temperature check (previous page of this
procedure)
IMPORTANT
If you dont get the right temperature, adjust it by the mean of the Reference
temperature (Diag.14)
REFERENCE
15:08
35.0
RUN CYCLE NUMBER 5
CHECK

MIN 33.0
MAX 37.0
Diag.14
When Reference temperature increases, reagent preheating temperature
increases
Make a thermostated compartment temperature check (previous page of this
procedure)
If required restart adjustment and check again, until you get an acceptable
temperature
RAS 244 A Ind.A
Page 7/7
P E N T R A 6 0
VACUUM ADJUSTMENT
CONCERNS
Concerns counting and draining syringe vacuum control and adjustment
REQUIRED TOOLS
Barflex
REQUIRED PRODUCTS
None
INTERVENTION TIME
15min
FREQUENCY
On request

None
RAS 245 A Ind.A
08/02/99
P E N T R A 6 0
VACUUM ADJUSTMENT
Page 2/5
DRAINING SYRINGE VACUUM CHECK

COUNTING SYRINGE VACUUM CHECK & ADJUSTMENT
NOTE
RAS 245 A Ind.A
VACUUM ADJUSTMENT
1 - Draining Syringe Vacuum Check

Open the right access door
Disconnect the tube on the draining syringe and replace it by the Barflex tube
(Diag.1)
Diag.1
Enter Menu: Maintenance\Technician\Vacuum Check\Draining\ (Diag.2&3)
TECHNICIAN\VACUUM CHECK
16:35
TECHNICIAN\VACUUM CHECK\DRAINING
1 - COUNTING
2 - DRAINING
16:35
CHECK VACUUM
Diag.2
Diag.3
Check vacuum on the Barflex, vacuum must be around 260mb and must be stable
NOTE
No adjustment available for draining syringe
Disconnect the Barflex and connect back the tube on the counting syringe
RAS 245 A Ind.A
P E N T R A 6 0
DRAINING SYRINGE VACUUM CHECK
Page 3/5
VACUUM ADJUSTMENT
P E N T R A 6 0
COUNTING SYRINGE VACUUM CHECK & ADJUSTMENT

1 - Counting Syringe Vacuum Check
WARNING !
Disconnect the lowest tube on the counting
syringe and replace it by the Barflex tube (Diag.4)
Diag.4
Enter Menu: Maintenance\Technician\Vacuum Check\Counting\Check (Diag.5&6)
TECHNICIAN\VACUUM CHECK
16:35
TECHNICIAN\VACUUM CHECK\COUNTING
VACUUM
16:35
850
1 - COUNTING
2 - DRAINING
CHECK
Diag.5
Diag.6
Check vacuum on the Barflex while the following screen is displayed, vacuum must
be around 220mb
VACUUM
16:35
850
CHECK VACUUM (220mb)

CHECK
Diag.7
NOTE
To get a better adjustment (difference of altitude for example) follow this

procedure
Disconnect the Barflex and connect back the tube on the counting syringe
Page 4/5
RAS 245 A Ind.A
VACUUM ADJUSTMENT
Diag.8
Enter Menu: Maintenance\Technician\Vacuum Check\Counting\Check (Diag.9)
Check vacuum on the Barflex, vacuum must be around 220mb
Press ESC and as in diagram 9, change the step value. If vacuum is too low
increase step value
VACUUM
16:35
50
CHECK
Diag.9
Repeat vacuum check and step value adjustment until you get the correct vacuum
Disconnect the Barflex and connect back the tube
RAS 245 A Ind.A
P E N T R A 6 0
2 - Counting Syringe Vacuum Adjustment

Open the left side panel
Disconnect the fourth tube from the top on the counting syringe and replace it by the
Barflex tube (Diag.8)
Page 5/5
P E N T R A 6 0

CONCERNS
Instrument dismantling:
Front panel
REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Torx keys
REQUIRED PRODUCTS
INTERVENTION TIME
30min
FREQUENCY
On request

None
RAS 247 A Ind.A
08/02/99
P E N T R A 6 0
FRONT PANEL
Page 2/5
NOTE
RAS 247 A Ind.A
1 - Front Panel Dismantling

Open the reagent door and remove the 4 bottles (disconnect tube on the stopper)
WARNING !
Open the cover
WARNING !
Fully lift sampling probe before pushing backward sampling carriage

Push the carriage back and unscrew the 2 Torx M3x16 screws (several turns) (Diag.1&2)
Diag.1
Diag.2
Unscrew the 2 CHC M3x6 screws (Diag.3)
P E N T R A 6 0
FRONT PANEL
Diag.3
RAS 247 A Ind.A
Page 3/5
P E N T R A 6 0
Unscrew the 4 CHC M3x10 screws (Diag.4)
Diag.4
Unscrew the 2 CHC M3x6 screws (just few turns) (Diag.5)
Diag.5
Disconnect the flat connector on the LCD & Keyboard board (Diag.6)
Diag.6
Lift the front panel and remove it carefully
Page 4/5
RAS 247 A Ind.A
RAS 247 A Ind.A
P E N T R A 6 0
2 - Front Panel Mounting

Reassemble following steps of chapter 1 backward
Run Menu: Maintenance\Autocontrol
Check that all is operating normally
Page 5/5
P E N T R A 6 0

CONCERNS
Replacement of the heater assy
REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Torx keys
REQUIRED PRODUCTS
INTERVENTION TIME
1h
FREQUENCY
On request

None
RAS 248 A Ind.A
08/02/99
P E N T R A 6 0
REPLACEMENT OF THE HEATER ASSY
Page 2/8
NOTE
RAS 248 A Ind.A
1 - Introduction
Heater assy has to be changed only when required
To access Heater assy you need to remove the Front panel, follow RAS 247 A Front
panel dismantling of this manual
Pull the Reagents syringe and the LMNE syringe to free space behind (no need to
disconnect tubes) Unscrew several turns the 4 silent block screws of each syring (Diag.1)
Diag.1
RAS 248 A Ind.A
P E N T R A 6 0
REPLACEMENT OF THE HEATER ASSY
Page 3/8
P E N T R A 6 0

2 - Heater Assy Dismantling
Unscrew the 2 CHC M3x6 screws shown on next diagram (Diag.2)
Diag.2
Disconnect following tubes on the Chambers assy:
- BASO 2
- RBC 1
- LMNE 2 & 3
- DIL 1
The heater assy is free, let it lay on the frame and by the front aperture disconnect
following tubes:
- From valve 22_2 (rep.1 on heater)
- From T4 (rep.5 on heater)
Diag.3
Diag.4
The heating system is now as shown on diagram 4
Page 4/8
RAS 248 A Ind.A
Diag.5
Take out the cable through the instrument
Take out the heater assy and drain it on absorbant paper
P E N T R A 6 0
Disconnect heating system wiring on the mother board plugged in J31: (Diag.5)
Diag.6
RAS 248 A Ind.A
Page 5/8
P E N T R A 6 0

3 - Heater Assy Mounting
Take new Heater assy and connect tubes on heater as follow:
NOTE
You can put the former and new heater beside and connect following
tubes one to one
REP. ON
HEAT ER
TO
Diluent chamber: DIL 1
LMNE chamber: LMNE 3
LMNE chamber: LMNE 2
RBC chamber: RBC 1
rep.11 of the heater
11
rep.9 of the heater
12
BASO chamber: BASO 2
Put new heater, with previous tubing connected, on instrument frame (Diag.8)
Connect following tubes: (Diag.9)
REP. ON
HEAT ER
TO
Valve 22 Inlet 2
Valve 8 Inlet 2
T4
Valve 22 Inlet 1
10
Valve 11 Inlet 2
Diag.8
Page 6/8
Diag.7
RAS 248 A Ind.A
Diag.9
Diag.10
Lift the heater assy, make the 5 tubes go through the chambers support steel sheet
(Diag.11)
Diag.11
Screw back the 2 heater assy screws (Diag.12)
P E N T R A 6 0
Dismantle chamber assy to get the tubes through the chambers support (Diag.10)
Diag.12
RAS 248 A Ind.A
Page 7/8
P E N T R A 6 0
Put back the chamber assy and connect the following tubes on chambers:
Page 8/8
- BASO 2
- RBC 1
- LMNE 2 & 3
- DIL 1
Connect heating system wiring on the mother board in J33: (Diag.13)
Diag.13
Push back the Reagents syringe and the LMNE syringe and screw silent blocks (check
that no tube is disconnected)
Put back the Front panel, follow RAS 247 A Front panel dismantling of this manual
Check chambers position, follow RAS 241 A Chambers Adjustment of this manual
When startup and prime are done check that there is no leak
RAS 248 A Ind.A
P E N T R A 6 0
BUBBLING ADJUSTMENT
CONCERNS
Bubbling
REQUIRED TOOLS
None
REQUIRED PRODUCTS
INTERVENTION TIME
15min
FREQUENCY
On request

None
RAS 249 A Ind.A
08/02/99
BUBBLING ADJUSTMENT
P E N T R A 6 0
BUBBLING ADJUSTMENT
Page 2/3
NOTE
RAS 249 A Ind.A
BUBBLING ADJUSTMENT
1 - Introduction
Bubbling is factory adjusted and normally does not required further adjustments
If an adjustment is necessary follow this procedure
2 - Bubbling Adjustment
Enter Menu: Maintenance\Technician\Bubbling\ (Diag.1)
Enter new step value for each bubbling
MAINTENANCE\TECHNICIAN\BUBBLING
16:05
1-AUTOCONTROL CYCLE
00
FIRST DILUTION
300
LMNE
WBC/BASO
300
RBC/PLT
300
ALPHALYSE
400
Diag.1
NOTE
When number of steps rises, bubbling is increasing
BUBBLING
LOW LIM IT
NORM AL
HIGH LIM IT
FIRST DILUTION
100
300
400
LMNE
100
300
400
WBC/BASO
100
300
400
ALPHALY SE
300
400
500
RAS 249 A Ind.A
P E N T R A 6 0
BUBBLING ADJUSTMENT
Page 3/3
P E N T R A 6 0

CONCERNS
Power supply
REQUIRED TOOLS
Hexagonal keys
Flat screw driver
REQUIRED PRODUCTS
INTERVENTION TIME
30min
FREQUENCY
On request

None
RAS 250 A Ind.A
08/02/99
P E N T R A 6 0
Page 2/5
NOTE
RAS 250 A Ind.A
1 - Power supply replacement (1xDBN 004 A)

Open the left side panel
Open the cover
Disconnect , on the mother board, the printer RS flat cable in J2 (Diag.1)
Diag.1
With a flat screwdriver disconnect, on the mother board, the supply connector in J37
(Diag.2)
Diag.2
Open the left side panel and the main board door (block it)
WARNING !
RAS 250 A Ind.A
P E N T R A 6 0
Page 3/5
P E N T R A 6 0
Disconnect the Optical bench lamp supply cable (Diag.3)
Diag.3
Unscrew the 2 CHC M3x6 back screws of the power supply (Diag.4)
Diag.4
Unscrew the 2 CHC M3x6 front screws of the power supply (Diag.5)
Diag.5
Page 4/5
RAS 250 A Ind.A
NOTE
Becareful to the lamp power supply cable when you remove the power
supply

Check following voltages:
T EST
POINT
TP40
TP41
TP42
TP43
DESIGNAT ION
5V Power supply
12V Power supply
24V Power supply
-12V Power supply
TARGET
+5V
+12V
+24V
-12V
P E N T R A 6 0
Take out the power supply
Diag.6
RAS 250 A Ind.A
Page 5/5
P E N T R A 6 0
START CYCLE
MICROSWITCH REPLACEMENT
CONCERNS
Start cycle microswitch replacement
REQUIRED TOOLS
Hexagonal keys
Torx keys
REQUIRED PRODUCTS
INTERVENTION TIME
30min
FREQUENCY
On request

None
RAS 251 A Ind.A
08/02/99
START CYCLE MICROSWITCH REPLACEMENT
P E N T R A 6 0
Page 2/4
NOTE
RAS 251 A Ind.A
1 - Introduction
Start cycle microswitch has to be changed or dismantle only when required
To access start cycle microswitch you need to remove the Front panel, follow RAS 247
A Front panel dismantling of this manual
2 - Start Cycle Microswitch Dismantling
Remove the thermostated compartment fan (Diag.1)
Diag.1
Release the Start cycle microswitch loosening the 2 screws (Diag.2)
Diag.2
P E N T R A 6 0
Diag.3
Disconnect the Start cycle microswitch (Diag.3)
RAS 251 A Ind.A
Page 3/4
P E N T R A 6 0
Page 4/4
3 - Start Cycle Microswitch Mounting

Connect the new Start cycle microswitch
IMPORTANT
Microswitch is connected Normally Open

Put back the Start cycle microswitch in the 2 slots (nearly full backward) (Diag.4)
Tighten the 2 microswitch screws
Diag.4
Check with the front panel in its location, that the Start cycle key is operating normally.
If not move forward or backward the microswitch until you get a correct location
NOTE
You must ear a click when key is pressed, and one when
key is released
Reinstall fan
WARNING !
Use the washer to connect ground wiring to the fan
Follow RAS 247 A Front panel dismantling of this manual to reassemble front panel
RAS 251 A Ind.A
P E N T R A 6 0

CONCERNS
Instrument maintenance:
Check up after intervention
REQUIRED TOOLS
Fresh and normal blood samples
Calibration blood samples
REQUIRED PRODUCTS
INTERVENTION TIME
1h
FREQUENCY
On request

None
RAS 252 A Ind.A
08/02/99
P E N T R A 6 0
CYCLE CHECK UP AFTER INTERVENTION
Page 2/5
NOTE
RAS 252 A Ind.A
1 - Preliminary
WARNING !
This procedure must be performed on a clean instrument

If instrument is suspected to be not perfectly clean, perform an Autoconcentrated
cleaning
WARNING !
Disposal gloves and white coat must be worn by the operator
Local or national regulations must be applied in all the operations
Run a STARTUP cycle
2 - Repeatability
Based on 20 consecutives analyses without alarm from one fresh and normal blood
sample
Run 20 consecutives analyses
Control to have variation coefficients within the following acceptable limits: (Diag.1)
PARAMETERS
% CV
TEST LEVEL
- WBC :
<2%
at 10.109 /l
- RBC :
< 2%
at 5.1012 /l
- HGB :
< 1%
at 15 g/dl
- HCT :
< 2%
at 45 %
- MCV :
< 1%
at 90 fl
- PLT :
< 5%
at 300.109 /l
Diag.1
X : Mean
Xi : Measure value
n : Measure number
SD : Standard deviation
X=
Xi
SD =
( X Xi )
n 1
CV calculated by means of the below formula:
CV (%) =
P E N T R A 6 0
CYCLE CHECK UP AFTER INTERVENTION
SD
x100
X
With one fresh and normal blood sample check that there is no alarm in CBC+5DIFF
mode
RAS 252 A Ind.A
Page 3/5
P E N T R A 6 0
3 - Calibration
Autocalibration:
Enter Menu: Calibration\Autocalibration\ (Diag.2)
Diag.2
Diag.4
IMPORTANT
Change Lot number, Expiration date or Target values if necessary
Run Calibration:
Prepare the calibrator according to the specific instructions (temperature, mixing, etc...)
Open the vial and position the sampling needle deeply inside the bottle
Press the sampling bar located behind the needle
When the cycle LED stops flashing, remove the vial and replace the cap on the calibrator
When the analysis cycle ends, the first result is displayed on the result chart table
Run the second calibrator sample
Diag.5
Page 4/5
Diag.3
RAS 252 A Ind.A
The calibration of the ABX PENTRA 60 can be performed on 3 to 11 analyses. The

autocalculation module performs statistics on these results in order to obtain the
best calibration coefficients.
In order to obtain the best calibration possible, it is recommended to run the
calibration blood a minimum of 5 times.
Calibration passed:
If the statistic figures are within the acceptable limits:
- Coefficient of variation is within the limits setup as described section and
- The percentage difference between the target and the mean value is less than
20
Press
to save the new coefficients (Diag.7)
Diag.6
Press
Diag.7
to print the new coefficients (Diag.8)
Diag.8
Check that the calibration coefficients remain within the following ranges: (Diag.9)
FDOLEUDWLRQ
FRHIILFLHQW
6WDQGDUG
YDOXH
PLQLPXP
PD[LPXP
WBC
137
90
200
RBC
225
160
290
HGB
40.0
25.0
55.0
HCT
220
160
290
PLT
290
180
400
RDW
0.3
0.1
0.9
RAS 252 A Ind.A
P E N T R A 6 0
IMPORTANT
Diag.9
Page 5/5
P E N T R A 6 0
STEP BY STEP ANALYSIS CYCLE CHECK

CONCERNS
Instrument control:
Analysis cycle
REQUIRED TOOLS
Fresh blood samples
REQUIRED PRODUCTS
INTERVENTION TIME
15min
FREQUENCY
On request

None
RAS 253 A Ind.A
08/02/99
P E N T R A 6 0
Page 2/8
STEP BY STEP ANALYSIS CYCLE INTRODUCTION

NOTE
RAS 253 A Ind.A
1 - Introduction
The main cycle consists of four principal phases:
1 - Preparation Of The Sample Phase: Dilutions
2 - Count And Measurement Of The WBC Group
3 - RBC/PLT Group Count
4 - Filling Of Tank
A special cycle is required when no other cycle is started during RBC/PLT count or
during the filling up of the tank:
5 - Rinsing Of The RBC/PLT Count Chamber
All phases are described in this procedure
2 - Cycle Start Condition
Run a startup cycle
Needle in the sampling position and green LED lit (Diag.1)
All chambers (execpt Rinse chamber) are filled with diluent (Diag.2)
Reagents syringe & LMNE syringe down (Diag.3)
Draining & Counting syringe down (Diag.3&4)
Diag.2
Diag.1
Draining
Syringe
Counting
Syringe
LMNE
Syringe
Reagents
Syringe
Diag.3
P E N T R A 6 0
STEP BY STEP ANALYSIS CYCLE INTRODUCTION
Diag.4
RAS 253 A Ind.A
Page 3/8
P E N T R A 6 0
Page 4/8
1 - Preparation Of The Sample Phase: Dilutions

First series of dilutions:
Press sampling bar, all the chambers are drained (Draining syringe)
1
Blood sample of 53 l (Sampling syringe)

Transfer of the needle toward the rinsing chamber (Sampling needle motor, Carriage
motor)
Distribution of 3 l of blood during the rinsing of the needle (Sampling syringe, Reagents
syringe)
Transfer of the needle from rinse chamber to the first dilution chamber (Sampling
needle motor, Carriage motor)
Distribution of 10 l of blood into a flux of 1.7 ml of diluent (Sampling syringe, Reagents
syringe)
Transfer of the needle from the first dilution chamber to the WBC and BASO count
chamber (Sampling needle motor, Carriage motor)
Distribution of 10 l of blood into a flux of 2 ml of BASOLYSE ll (Sampling syringe,
Reagents syringe)
Transfer of the needle from the WBC and BASO count chamber to the LMNE mixing
Distribution of 25 l of blood into a flux of 1 ml of EOSINOFIX (Sampling syringe,
Reagents syringe)
Transfer of the needle from the LMNE mixing chamber to the rinse chamber (Sampling
Distribution of the 5 l of residual blood during the double rinsing (interior and exterior) of
the needle (Sampling syringe, Reagents syringe)
NOTE
Realisation of the LMNE dilution:

The dilution in the LMNE chamber is completed by 1 ml of diluent after 12
seconds of incubation:
Positioning of the needle opposite the distribution nipple for the
EOSINOFIX
Control of valve 8 then set the syringe group for a dose of 1 ml
Simultaneously: Setting of the distribution syringe to distribute 25 l of
blood
Stop valve 8
Incubation for 12 seconds
Control of valves 1 and 3 and setting of the LMNE injection syringe to
distribute 1 ml of the stop diluent
Stop valves 1 and 3
RAS 253 A Ind.A
Sample of 42.5 l of the first dilution (Sampling syringe)

Rinsing of needle exterior (in the first dilution chamber) with 0.4 ml of diluent (Reagents
syringe)
Transfer of the needle from the first dilution chamber to the RBC and PLT count
Distribution of the 42.5 l of first dilution into a flux of 2 ml of diluent (Sampling needle
motor, Carriage motor)
Distribution of 0.5 ml of diluent from the interior of the needle (Sampling needle motor,
Carriage motor)
NOTE
In the case of this RBC/PLT dilution, it is the entire blood sample that
is distributed (and not a portion as in the preceding dilution). The distribution in a flux is kept in order to benefit from the homogenisation aspect of the procedure
In order to attain the hemoglobin measurement we add 0.4 ml of
lysing agent into the first dilution chamber immediately following the
blood sample for the RBC/PLT dilution1
RAS 253 A Ind.A
P E N T R A 6 0
Second dilution for the RBC and the PLT:

Transfer of the needle from the rinse chamber to the first dilution chamber (Sampling
2
Page 5/8
P E N T R A 6 0
Page 6/8
2 - Count And Measurement Of The WBC Group

LMNE:
Loading of the vacuum injection circuit of the optical bench (Draining syringe, control of
valves 4 and 5)
Start flux in the optical chamber (approximately 2 seconds)
Injection, acquisition of the matrix and WBC count for 12 seconds
Simultaneously: WBC and BASO counts:
Rinsing of the counting head (Diluter pushed into position, control of valves 9, 10, 21, 23
and 13)
Start the vacuum and first part of count (duration of 6 seconds, control of valve 23)
Rinsing of counting head and adjustment of the vacuum count
Second part of count (duration of 6 seconds)
After this first series of measurements:
Measurement of the hemoglobin
Rinsing of the WBC/BASOS count chamber with a mixture of 1.06 ml of ABX UNICLEAN and
1.44 ml of diluent ABX DIL
Rinsing of the WBC/BASOS counting head
RAS 253 A Ind.A
First series of counts (duration of 5 seconds, control valve 14).

Rinsing of the RBC/PLT counting head and adjustment of the vacuum.
Second series of counts (duration of 5 seconds)
NOTE
P.R.F. Process (Permanent Rinse Flow)

The WBC/BASOS and the RBC/PLT counting heads are in series during
the RBC/PLT count. The count flux from the WBC chamber circulates in
the RBC/PLT count head and limits the possibility of the circulation of the
RBC behind the count orifice.
Autocleaning of the WBC ruby
The P.R.F. allows as well an auto cleaning of the WBC/BASOS inasmuch
as a UNICLEAN/DIL (cleaning solution) mixture is drawn through the WBC/
BASO ruby during the entire duration of the RBC and PLT counts
4 - Filling Of Tank
At the end of the RBC/PLT count, the RBC count vacuum is finished and used for the
filling up of the diluent tank (control valves 15 and 25)
P E N T R A 6 0
3 - RBC/PLT Group Count

Rinsing of the RBC/PLT counting head
Start the vacuum for the RBC/PLT counting head
In order to improve the filling speed, the syringe is initialised for a few seconds then drawn
again to complete the filling
A float located in the tank controls the level of liquid
RAS 253 A Ind.A
Page 7/8
P E N T R A 6 0
Page 8/8
5 - Rinsing Of The RBC/PLT Count Chamber

For reasons of maximising the mechanical resources, the rinsing of the RBC/PLT chamber
is normally programmed for the beginning of the succeeding cycle (which can be started
during the RBC and PLT count)
If no other cycle is started during RBC/PLT count or during the filling up of the tank, a
special rinsing sequence is initiated. The sample signal light changes to red and the instrument is jammed for a few seconds
This sequence involves draining of the RBC chamber followed by a refilling with the diluent
RAS 253 A Ind.A
P E N T R A 6 0
OPTICAL BENCH
DISMANTLING & REPLACEMENT
CONCERNS
Dismantling, Replacement and Control of Optical Bench
REQUIRED TOOLS
Hexagonal keys
Flat screw driver
Torx keys
REQUIRED PRODUCTS
INTERVENTION TIME
30min
FREQUENCY
On request

None
RAS 254 A Ind.A
08/02/99
P E N T R A 6 0
OPTICAL BENCH
Page 2/6
DISMANTLING & REPLACEMENT OF THE OPTICAL BENCH

OPTICAL BENCH CONTROL
NOTE
RAS 254 A Ind.A
OPTICAL BENCH
1 - Optical Bench Dismantling

WARNING !
Open the cover
Disconnect tubes from LMNE syringe:
- Inlet 1
- Inlet 5
Disconnect tubes from valves:
- 1 Inlet 1
- 4 Inlet 2
Disconnect tube from ground fitting (Diag.1)
Diag.1
Disconnect following connections: (Diag.2)
- Lamp supply
- LMNE CIS & LMNE OD coaxes
- Optical pre-ampli board supply
- Optical bench grounding wire
P E N T R A 6 0
DISMANTLING & REPLACEMENT OF THE OPTICAL BENCH
Diag.2
RAS 254 A Ind.A
Page 3/6
P E N T R A 6 0
OPTICAL BENCH
Page 4/6
Remove gently the Optical bench

Untighten the silent blocks (5mm allen key) (Diag.3)
Diag.3
2 - Optical Bench Replacement

Locate new Optical bench
Tighten the silent blocks (5mm allen key) (Diag.3)
NOTE
Do not tighten silent blocks too hard, check that optical bench Cannot be
lifted
Connect all tubing and wiring as described in Optical Bench Dismantling
RAS 254 A Ind.A
OPTICAL BENCH
1 - Emission Gun Location Control

Open Optical bench cover (Diag.4)
Diag.4
Check that gap between emission gun and flowcell is around 3mm (Diag.5)
WARNING !
Becareful, if you use a 3mm allen key to control gap, that you dont damage flowcell
or emission gun
Diag.5
WARNING !
P E N T R A 6 0
OPTICAL BENCH CONTROL
This adjustment is factory made and must not be changed
RAS 254 A Ind.A
Page 5/6
P E N T R A 6 0
OPTICAL BENCH
2 - Optical Bench Lamp Alignement Control
Open Optical bench cover (Diag.6)
Diag.6
To control lamp put a piece of white paper between reception gun and flowcell (Diag.7)
Diag.7
Check that projection of lamp filament is vertical (Diag.8)
Diag.9
Diag.8
WARNING !
A small screw (1.2mm allen key) allows adjutment of lamp (Diag.9)

This adjustment is factory made and must not be changed
Page 6/6
RAS 254 A Ind.A
P E N T R A 6 0
BARCODE READER CONFIGURATION

CONCERNS
Barcode Reader test and configuration
REQUIRED TOOLS
None
REQUIRED PRODUCTS
INTERVENTION TIME
15min
FREQUENCY
On request

None
RAS 255 A Ind.A
17/09/99
P E N T R A 6 0
BARCODE READER TEST
Page 2/4
RAS 255 A Ind.A
1 - Test
Check that barcode reader is working properly by means of the reading test (Diag.1)
READ TEST for DATALOGIC

WITH CHECKSUM
WITHOUT CHECKSUM
EAN 8
C39
1234567
TEST
EAN 8
2/5
9076543210
1234567
CODABAR
EAN 13
543210
123456789012
C128
EAN 13
0123456789
123456789012
If the reading test is correct, the procedure is completed
RAS 255 A Ind.A
P E N T R A 6 0
BARCODE READER TEST
Diag.1
Page 3/4
P E N T R A 6 0
Page 4/4
2 - Procedure to carry out in case of reading test failure

Switch on the instrument and within the 15 first seconds, read from the top to the
bottom the following barcode labels on this page. The audible beep occurs after each
reading. Read all the labels from top to bottom and from left to right
When the last label is read, check once more on the test labels (Diag.1)
WARNING !
For code I2 of 5, to avoid a bad interpretation, it is mandatory to hold the reader in
order to read the entire code
If the reading test has failed for the DATALOGIC reader, proceed to the barcode setup as
follows:
$+CQ0$-
$+CP0$-
AB11AB*0116
EB3EHB54FF
AC110416
EB3EHNFFFF
AI11
EB3EHVFFFF
$+
AD111AD*0316
EB3EHRFFFF
CA0CC1CE2
EA110DEC0
EB3EHTFFFF
AZ0AA3
EB3EHA45FF
$-
$+$*
RAS 255 A Ind.A
9. TRAINING SLIDES
Content
10. EXPLODED VIEWS
Content
1.
Blowups views........................................................................... 1
1.1.
1.2.
1.3.
1.4.
1.5.
1.6.
1.7.
1.8.
1.9.
1.10.
1.11.
1.12.
1.13.
1.14.
1.15.
1.16.
1.17.
1.18.
1.19.
1.20.
1.21.
1.22.
1.23.
1.24.
1.25.
1.26.
1.27.
1.28.
1.29.
1.30.
1.31.
1.32.
1.33.
1.34.
1.35.
1.36.
1.37.
1.38.
1.39.
1.40.
1.41.
1.42.
1.43.
1.44.
1.45.
11 Valves assy ................................................................................... 1

5 Valves assy (12-16) ...................................................................... 2
2 Valves assy .................................................................................... 3
7 Valves assy .................................................................................... 4
5 Valves assy (27-31) ..................................................................... 5
Optical bench syringe assy ........................................................... 6
Reagents syringe assy ................................................................... 7
Sampling syringe assy .................................................................... 8
Syringe motorization carriage assy .......................................... 9
Syringe motor assy ...................................................................... 10
Syringe motor body assy ............................................................ 11
Sampling motor assy .................................................................... 12
Optical syringe motor assy ........................................................ 13
Reagents motor assy ................................................................... 14
Sampling syringe assy .................................................................. 15
Vacuum syringe piston assy ........................................................ 16
Vacuum syringe assy ..................................................................... 17
Short vacuum syringe assy ......................................................... 18
Long vacuum syringe ..................................................................... 19
Long vacuum syringe assy .......................................................... 20
Short vacuum syringe assy ......................................................... 21
Needle carriage motor assy ..................................................... 22
Horizontal sampling carriage notched belt .......................... 23
Notched belt ................................................................................ 24
Needle carriage assy .................................................................. 25
Sampling horizontal carriage .................................................... 26
Sampling horizontal carriage assy .......................................... 27
Sampling carriage assy ............................................................... 28
Carriage vertical belt ................................................................. 29
Carriage rinsing block assy ........................................................ 30
Home carriage cell ....................................................................... 31
Needle assy ................................................................................... 32
Carriage motor assy .................................................................... 33
Sampling needle assy .................................................................. 34
Reagents heating coils assy ...................................................... 35
Thermostated room assy .......................................................... 36
Thermostated room plate assy ............................................... 37
Thermostated room fan assy ................................................... 38
Frame assy ..................................................................................... 39
Diluent chamber assy .................................................................. 40
Diluent chamber ............................................................................ 41
RETIC flowcell assy ................................................................... 42
Optical bench assy ....................................................................... 43
Optical bench lamp ...................................................................... 44
Preamplifier support ................................................................... 45
11. SPARE PART LIST

Content
REF
DESIGNATION
PACKAGING
CATEGORY
CAE006A
SWITCH,MICROSWITCH XC5-81-82
SINGLE USE
CAE010A
SWITCH,MICROSWITCH XC5-81
SINGLE USE
DAC011A
CABLE,POWER EUROPE
SINGLE USE
DAC012A
CABLE,POWER USA
SINGLE USE
DAJ007A
LAMP,ASSY FOR OPTIC BENCH P60
CONSUMABLE
DBH001A
TY RAP,SMALL
PACK OF 1000
CONSUMABLE
DBH002A
TY RAP,BIG
PACK OF 1000
CONSUMABLE
DBK004A
ADHESIVE,HOLDER TY RAP D=3MM
PACK OF 10
CONSUMABLE
DBN004A
PCB,POWER SUPPLY BLOCK P60
EAC008A
FITTING,ANTI ROTATION WASHER
PACK OF 5
CONSUMABLE
EAC010A
FITTING,LUER FEMALE I=3MM
PACK OF 5
CONSUMABLE
EAC019A
FITTING,LUER MALE I=3MM
PACK OF 5
CONSUMABLE
EAE005AS
TUBING,TY GON 1,016MM (0,040)
PACK OF 2M
CONSUMABLE
EAE006AS
PACK OF 2M
CONSUMABLE
EAE007AS
PACK OF 2M
CONSUMABLE
EAE008AS
PACK OF 2M
CONSUMABLE
EAE011AS
TUBING,CRY STAL 3X6
PACK OF 10M
CONSUMABLE
EAE028AS
TUBING,CRY STAL 4X6
PACK OF 2M
CONSUMABLE
EAE034AS
TUBING,TY GON 2.54MM (0,100)
PACK OF 2M
CONSUMABLE
EAE051AS
TUBING,SILICONE 4,5 X 8
PACK OF 2M
CONSUMABLE
FAA040A
O'RING,5DIFF SY RINGE D=12,1
PACK OF 10
CONSUMABLE
FAA046A
O'RING,COAXIAL CABLE
PACK OF 10
CONSUMABLE
FAA053A
O'RING,SAMPL. NEEDLE MICROS OT
PACK OF 10
CONSUMABLE
FAA064A
O'RING,SAMPLING SY RINGE P60
PACK OF 10
CONSUMABLE
FAA065A
O'RING,REAGENT SY RINGE D=6,3
PACK OF 10
CONSUMABLE
FAA066A
O'RING,DRAINING CHAMBER P60
PACK OF 12
CONSUMABLE
FAA067A
O'RING,5DIFF SY RINGE D=2,4
PACK OF 10
CONSUMABLE
FAK001A
CHAMBER,APERTURE 50
CONSUMABLE
FAK003A
CHAMBER,APERTURE 80
CONSUMABLE
FAL009A
SILENT BLOC,FOR SUB ASSEMBLIES
PACK OF 12
CONSUMABLE
FAL010A
SILENT BLOC,OPTIC BENCH P60
PACK OF 12
CONSUMABLE
FAM004A
COVER,PLASTIC FOOT FOR CHASSIS
PACK OF 8
CONSUMABLE
FBH016A
COVER,DUST COVER PENTRA60
CONSUMABLE
FBL001A
REAGENT,REAGENT CAP 2 HOLES
CONSUMABLE
FBR011A
BELT,NEEDLE L=364 P60
CONSUMABLE
FBR012A
BELT,CARRIAGE L=544 P60
CONSUMABLE
FZZ024A
DOOR,PUSH PUSH P60
PACK OF 3
CONSUMABLE
GAK302A
REAGENT,BOTTLE STOPPER D=40
PACK OF 12
CONSUMABLE
GAL094A
CHAMBER,WAST.P120/DIL TANK P60
STD EXCH
SINGLE USE
REF
DESIGNATION
PACKAGING
CATEGORY
GBC015A
CLIP,MIX CH. HOLDER MIC/P120
PACK OF 10
CONSUMABLE
GBC030A
SY RINGE,REAGENT PISTON MIC/P60
SINGLE USE
GBC031A
SY RINGE,LY SE PISTON MIC/P60
SINGLE USE
GBG003A
CHAMBER,DRAINING 1/4 TURN P60
SINGLE USE
GBG007A
CHAMBER,DRAINING CHICANE P60
GBG013A
CHAMBER,OVERFLOW CUP T ROOM
CONSUMABLE
GBG022A
CHAMBER,T ROOM COVER P60
SINGLE USE
GBG023A
PROTECTION,FOR VALVES T ROOM
CONSUMABLE
GBG033A
SY RINGE,REAGENT BLOCK BODY P60
SINGLE USE
GBG037A
SY RINGE,5DIFF BLOCK BODY P60
SINGLE USE
GBG040A
SY RINGE,5DIFF PISTON P60
SINGLE USE
GBG042A
SY RINGE,5DIFF CROSSPIECE P60
CONSUMABLE
GBG044A
SY RINGE,SAMPLING SY R. BODY P60
SINGLE USE
GBG048A
SY RINGE,SAMPLING CROSSPIECE
CONSUMABLE
GBG052A
SY RINGE,VAC/WASTE PISTON P60
SINGLE USE
GBG053A
SY RINGE,VACCUM PUMP BODY P60
SINGLE USE
GBG054A
SY RINGE,WASTE PUMP BODY P60
SINGLE USE
GBG090A
CARRIAGE,RINSING BLOCK P60
SINGLE USE
GBG091A
CARRIAGE,NEEDLE GUIDE P60
SINGLE USE
GBG093A
MOTOR,PULLEY P60
CONSUMABLE
GBG096A
KEY BOARD,SILICON
SINGLE USE
GBG100A
STICKER,FRONT PANEL P60
CONSUMABLE
GBG130A
COVER,TOP COVER P60
COVER
GBG137A
COVER,LEFT SIDE COVER P60
COVER
GBG138A
DOOR,KEY FOR T ROOM P60
SINGLE USE
GBG144A
REAGENT,STRAW D=27 P60
ACCESSORIES
GBG145A
REAGENT,STRAW D=20 P60
ACCESSORIES
GBG147A
CUP,OVERFLOW REAGENTS SY R. P60
CONSUMABLE
GBG155A
REAGENT,BOTTLE STOPPER D=25
PACK OF 4
CONSUMABLE
GBG156A
O'RING,APERTURE P60
PACK OF 12
CONSUMABLE
GBG157A
CHAMBER,COUNTING HEAD P60
HAE026B
STICKER,VALVES JOUCO (1-10)
PACK OF 10
CONSUMABLE
HAE027B
PACK OF 10
CONSUMABLE
HAE028B
PACK OF 10
CONSUMABLE
HAE029B
PACK OF 10
CONSUMABLE
HAW002A
STICKER,FOR REAGENT DOOR P60
CONSUMABLE
MAB090A
TOOL,TORX KEY T10
TOOL
NAA068A
STICKER,FOR TUBING (169 ITEMS)
CONSUMABLE
RAA016A
MANUAL,TECHNICAL PENTRA60
TOOL
PACK OF 4
CONSUMABLE
SINGLE USE
REF
DESIGNATION
PACKAGING
CATEGORY
RAB080A
MANUAL,USER'S PENTRA60 GB
TOOL
RAC046A
MANUAL,USER'S PENTRA60 FR
TOOL
RAP012A
MANUAL,SPLIT VIEWS PENTRA60
TOOL
XAA398A
PRINTER,EPSON LX300 220V
PERIPHERAL
XAA399A
PRINTER,EPSON LX300 110V
PERIPHERAL
XAA423BS
PCB,PREAMP+MECA ASSY PENTRA60
STD EXCH
XAA425BS
PCB,MAIN BOARD PENTRA60
STD EXCH
XAA427BS
PCB,LINK BOARD PENTRA60
STD EXCH
XAA429A
PCB,LED BOARD FOR COVER P60
STD EXCH
XAA444A
PCB,LCD SCREEN EQUIPPED P60
STD EXCH
XBA144A
CABLE,REAGENT GROUND FITTING
CONSUMABLE
XBA193A
CABLE,COAX BASO-O.D LMNE-LMNE
CONSUMABLE
XBA386A
CABLE,T ROOM HARNESS PENTRA60
SINGLE USE
XBA389A
PHOTOMETER,HB CPTE PENTRA60
SINGLE USE
XBA390A
MOTOR,FOR SY RINGE BLOCKS P60
SINGLE USE
XBA391A
MOTOR,CARRIAGE PENTRA60
SINGLE USE
XBA392A
MOTOR,SAMPLING NEEDLE PENTRA60
SINGLE USE
XBA393A
FAN,MAIN FAN 24V PENTRA60
SINGLE USE
XBA394A
SENSOR,CARRIAGE MOVE PENTRA60
SINGLE USE
XBA395A
SENSOR,DRAINING DETECTION P60
SINGLE USE
XBA396A
SENSOR,SAMPL. NEEDLE MOVE P60
SINGLE USE
XBA398A
CABLE,COAX RBC/WBC PENTRA60
CONSUMABLE
XBA399A
CABLE,COAX LMNE FLOWCELL P60
CONSUMABLE
XBA403A
TUBING,SHIELD REPARTITOR P60
CONSUMABLE
XCA166A
CHAMBER,ISOLATOR (LONG)
CONSUMABLE
XCA167A
CHAMBER,ISOLATOR (SMALL)
CONSUMABLE
XDA481B
VALVE,LIQ. 2WAY S/NC W/O COIL
SINGLE USE
XDA483B
VALVE,LIQ. 3WAY S W/O COIL
SINGLE USE
XDA555AS
TOOL,FLOWCELL ADJ. KNOB
TOOL
XDA591AS
SY RINGE,5DIFF ASSY PENTRA60
SINGLE USE
XDA592AS
SY RINGE,REAGENT ASSY PENTRA60
SINGLE USE
XDA593AS
SY RINGE,SAMPLING ASSY P60
SINGLE USE
XDA594AS
MOTOR,BLOCK FOR 5DIFF SY R. P60
SINGLE USE
XDA595AS
MOTOR,BLOCK FOR REAG. SY R. P60
SINGLE USE
XDA596AS
MOTOR,BLOCK FOR SAMPL. SY R.P60
SINGLE USE
XDA597AS
SY RINGE,WASTE COMPLETE P60
SINGLE USE
XDA598AS
SY RINGE,VACCUM COMPLETE P60
SINGLE USE
XDA600A
OPTICAL,BENCH PENTRA60
STD EXCH
XDA601AS
CHAMBER,LMNE FLOWCELL PENTRA60
SINGLE USE
REF
DESIGNATION
PACKAGING
CATEGORY
XDA602A
CHAMBER,4 CHAMBERS BLOCK P60
SINGLE USE
XDA605A
CHAMBER,DILUENT TANK ASSY P60
SINGLE USE
XDA610A
CHAMBER,WBC/BASO CPTE PENTRA60
SINGLE USE
XDA611CS
VALVE,LIQ 11 VALVE ASSY (1-11)
SINGLE USE
XDA612CS
SINGLE USE
XDA613CS
SINGLE USE
XDA614CS
SINGLE USE
XDA615CS
SINGLE USE
XDA616AS
NEEDLE,190L 5DIFF SY R. P60
CONSUMABLE
XDA617AS
NEEDLE,100L SAMPLING SY R. P60
CONSUMABLE
XDA618AS
CARRIAGE,NEEDLE CARRIAGE P60
SINGLE USE
XDA619AS
NEEDLE,SAMPLING NEEDLE P60
CONSUMABLE
XDA621A
O'RING,VAC/WASTE PUMP + WASHER
PACK OF 10
CONSUMABLE
XDA622A
O'RING,REAGENT SY R. + WASHER
PACK OF 10
CONSUMABLE
XDA624AS
DOOR,FOR T ROOM EQUIPPED P60
SINGLE USE
XDA625AS
HEATER,BLOCK COMPLETE PENTRA60
SINGLE USE
XDA626AS
CHAMBER,DILUENT TANK COVER P60
SINGLE USE
XDA627AS
PCB,KEY BOARD/LCD DISP ASSY P60
STD EXCH
XDA628AS
COVER,FRONT COVER EQUIPED P60
SY STEM COVER
XDA629AS
COVER,REAGENT DOOR EQUIPED P60
SY STEM COVER
XEA018A
REAGENT,STRAW DILUENT L=360
CONSUMABLE
XEA019A
GREASE,SILICON CONTAINER
CONSUMABLE
XEA286AS
KIT,O'RING + WASHER P60/P120
CONSUMABLE
XEA311AS
KIT,FITTINGS
CONSUMABLE
XEA484A
KIT,INSTALLATION PENTRA60
CONSUMABLE
XEA485AS
KIT,MAINTENANCE P60 6 MONTHS
CONSUMABLE
XEA486AS
KIT,MAINTENANCE P60 1 Y EAR
CONSUMABLE
XEA487AS
KIT,T ROOM FAN + HARNESS P60
SINGLE USE
XEA488AS
KIT,HEATER HARNESS P60
SINGLE USE
XEA581AS
KIT,SY RINGE PISTONS KIT P60
SINGLE USE

ABX Pentra 60 - Service Manual

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ABX Pentra 60 - Service Manual

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ABX HEMATOLOGIE

P/N REVISION REVISION

RAA 016 A Ind A

Technical specifications ............................................................................................. 2

Physical specifications ................................................................................................ 4

Reagents specifications ............................................................................................. 8

Power requirements ..................................................................................................................... 4

Summary of performance data ................................................................................. 6

Diluent: ABX DILUENT .............................................................................................................. 8

RAA 016 A Ind A

2-Hydraulic & Pneumatic principles

Pentra 60 Hydraulic .................................................................................................... 2

Transmission tube list ................................................................................................................. 2

Pneumatic diagrams ..................................................................................................... 8

Pentra 60 general pneumatic diagram ..................................................................................... 8

RAA 016 A Ind A

3-Electric & Electronic principles

Pentra 60 Synoptic ...................................................................................................... 2

Pentra 60 Main Board ................................................................................................. 3

board general view ............................................................................................................. 3

Pentra 60 Boards ......................................................................................................... 9

Optical preampli board XAA 423 B ......................................................................................... 9

Horizontal Carriage Motor XBA 391 A ................................................................................. 14

RAA 016 A Ind A

4-Analysis Cycle Technology

Analysis cycle description ......................................................................................... 2

Measuring principles ................................................................................................... 4

Multi Distribution Sampling System (MDSS) ....................................................................... 4

RAA 016 A Ind A

2. Software Release ............................................................................................................. 4

RAA 016 A Ind A

Argos format principles ............................................................................................. 2

ABX format principles ................................................................................................ 5

Message structure ....................................................................................................................... 6

Pin assignments .......................................................................................................... 21

RAA 016 A Ind A

7-Alarms & Error List

Error messages ............................................................................................................ 2

RAA 016 A Ind A

Maintenance kits .......................................................................................................... 4

6 month Maintenance kit ............................................................................................................ 4

Procedure chart tables .............................................................................................................. 11

RAA 016 A Ind A

RAA 016 A Ind A

Blowups views ................................................................................................................. 1

11 Valves assy ................................................................................................................................. 1

RAA 016 A Ind A

11-Spare part list

Spare part list ...............................................................................................................1

RAA 016 A Ind A

ABX Diagnostics Training & Documentation Center

If you have some modifications, requests or adds to do to this manual,

ABX Diagnostics Technical Informations

REQUEST FORM FOR MODIFICATIONS OR

To be filled in by the documentation department

Requested person informed on :

Technical specifications ....................................................... 2

Physical specifications .......................................................... 4

Reagents specifications ........................................................ 8

Power requirements ....................................................................... 4

Summary of performance data ........................................... 6

Diluent: ABX DILUENT ................................................................. 8

RAA 016 A Ind A

CBC + 5DIFF Mode