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Growth is shown as L = log(numbers) where numbers is the number of colony forming units per
ml, versus T (time.)
Bacterial growth is the asexual reproduction, or cell division, of a bacterium into two daughter
cells, in a process called binary fission. Providing no mutational event occurs the resulting
daughter cells are genetically identical to the original cell. Hence, "local doubling" of the
bacterial population occurs. Both daughter cells from the division do not necessarily survive.
However, if the number surviving exceeds unity on average, the bacterial population undergoes
exponential growth. The measurement of an exponential bacterial growth curve in batch culture
was traditionally a part of the training of all microbiologists; the basic means requires bacterial
enumeration (cell counting) by direct and individual (microscopic, flow cytometry[1]), direct and
bulk (biomass), indirect and individual (colony counting), or indirect and bulk (most probable
number, turbidity, nutrient uptake) methods. Models reconcile theory with the measurements.[2]
Contents
1 Phases
2 See also
3 References
4 External links
Phases
This basic batch culture growth model draws out and emphasizes aspects of bacterial growth
which may differ from the growth of macrofauna. It emphasizes clonality, asexual binary
division, the short development time relative to replication itself, the seemingly low death rate,
the need to move from a dormant state to a reproductive state or to condition the media, and
finally, the tendency of lab adapted strains to exhaust their nutrients. In reality, even in batch
culture, the four phases are not well defined. The cells do not reproduce in synchrony without
explicit and continual prompting (as in experiments with stalked bacteria [5]) and their
exponential phase growth is often not ever a constant rate, but instead a slowly decaying rate, a
constant stochastic response to pressures both to reproduce and to go dormant in the face of
declining nutrient concentrations and increasing waste concentrations.
Batch culture is the most common laboratory growth method in which bacterial growth is
studied, but it is only one of many. It is ideally spatially unstructured and temporally structured.
The bacterial culture is incubated in a closed vessel with a single batch of medium. In some
experimental regimes, some of the bacterial culture is periodically removed and added to fresh
sterile medium. In the extreme case, this leads to the continual renewal of the nutrients. This is a
chemostat, also known as continuous culture. It is ideally spatially unstructured and temporally
unstructured, in a steady state defined by the rates of nutrient supply and bacterial growth. In
comparison to batch culture, bacteria are maintained in exponential growth phase, and the
growth rate of the bacteria is known. Related devices include turbidostats and auxostats.
Bacterial growth can be suppressed with bacteriostats, without necessarily killing the bacteria. In
a synecological, true-to-nature situation in which more than one bacterial species is present, the
growth of microbes is more dynamic and continual.
Liquid is not the only laboratory environment for bacterial growth. Spatially structured
environments such as biofilms or agar surfaces present additional complex growth models.
See also
Demographic transition
References
1.
Skarstad K, Steen HB, Boye E (1983). "Cell cycle parameters of slowly growing Escherichia
coli B/r studied by flow cytometry". J. Bacteriol. 154 (2): 65662. PMC 217513.
PMID 6341358.
External links
This article includes material from an article posted on 26 April 2003 on Nupedia; written by
Nagina Parmar; reviewed and approved by the Biology group; editor, Gaytha Langlois; lead
reviewer, Gaytha Langlois ; lead copyeditors, Ruth Ifcher. and Jan Hogle.
Categories:
Bacteriology
Population
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