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Clear aim
Well designed study should include clearly stated hypothesis or aim.
Design of study must be appropriate to stated hypothesis or aim and
produce results that are valid and reliable.
Representative sample
Representative sample must be selected form wider population that
the studys conclusion will be applied to.
Selection bias occurs when those who participate in the study are not
representative of the target population.
Differences between people asked to take part in a study and those
who actually respond should also be considered before generalising
findings to the target population.
The proportion of individuals who drop out of a study after it has begun
should be kept to a minimum.
Particularly important for cohort studies which follow people over long
periods of time.
Peoples who drop out of studies often share common features.
Important to monitor characteristics of remaining participants to
ensure that they are still representative of the target population.
Valid and reliable results
Any methods used must produce valid data, from measurement that
provide information on what the study set out to measure.
Method used to collect results must be reliable.
A reliable method used at different times, or by different people will
produce similar results.
Disease diagnosis must be clearly defined to ensure different doctors
record and measure symptoms in the same way.
A sample must be large enough to produce results that could not have
occurred by chance.
With larger samples more reliable estimates for the wider population
can be calculated.
Potential effect of all variables that could be correlated with the disease
should be considered when designing the study.
Matching case and control groups on variables known to correlate with
the disease being studied will ensure that only the factor under
investigation is influencing the outcome.
Pressure reading taken when blood first starts to spurt through artery
that has been closed. This is the systolic pressure.
Second reading taken when pressure falls to point where no sound
heard in artery. This is the diastolic pressure.
Blood pressure reported as two numbers one over other eg 140/85.
This means systolic pressure of 140 mmHg and diastolic pressure of 85
mmHg. For average healthy person expect systolic pressure of between
100-140 and diastolic pressure between 60-90.
Glucose important- main sugar used by all cells in respiration. Starch &
glycogen made up of glucose subunits. When starch & glycogen
digested form glucose which is reabsorbed and transportes in cells,
Galactose occurs in our diet as part of disaccharide lactose found in
milk.
Fructose occurs naturally in fruit, honey.
Monosaccarides rapid source of energy readily absorbed.
Disaccharides
Two single sugar units join together to form dissacharide in
condensation reaction.
Condensation reaction water molecule released as two sugar
molecules combine in reaction.
Bond that forms between two glucose molecules known as a glycosidic
bond.
Bond in maltose known as 1.4 glycosidic bond as forms between
carbon 1 on one molecule and carbon 4 on other.
Sucrose- formed from glucose and fructose, usual form of sugar
transport in plants
Maltose- formed from two glucose molecules disaccharide produced
when amylase breaks down in starch.
Lactose- galactose and glucose make up lactose- sugar found in milk
Monosaccharides eaten rapidly absorbed into blood sharp rise in
blood sugar.
Polysaccharides and disaccharides have to be digested into
monosaccharides before absorption.
Takes time monosaccharides released slowly eating complex
carbohydrates does not cause swings in blood sugar levels as does
monosaccharides.
Lactose sugar present in milk. Many adults intolerant to lactose.
Solution is to hydrolyse milk converts disaccharide lactose into
monosaccharides glucose and galactose.
Industrially carried out using enzyme lactase. Lactase immobilised in
gel, milked poured in continuous stream through column containing
beads of immobilised enzyme.
Polysaccharides
3 main types of polysaccharide found in food: starch and cellulose in
plants and glycogen in animals. Although polymers of glucose molecule
they are sparing soluble and do not taste sweet.
Starch and glycogen energy storage molecules in cells.
These polysaccharides are suitable for storage as are compact and low
solubility in water.
Do not affect concentration of water in cytoplasm and do not affect
water movement in and out of cell by osmosis.
Starch- storage carbohydrate found in plants made up of mixture of
two molecules amylose and amylopectin.
During reactions in the body unstable radicals result when an atom has
an unpaired electron. Radicals are highly reactive and can damage
enzymes and genetic material.
This type of cell damage has been implicated in development of some
types of cancer, heart disease and premature ageing.
Some vitamins including vitamin C, beta carotene and vitamin E can
protect against the radical damage.
They provide hydrogen atoms and stabilise the radical by pairing up its
unpaired electron.
Salt
High salt diet causes kidney to retain water.
Higher fluid levels in blood result in elevated blood pressure with
associated CVD risks.
Stress
Evidence that CHD sometimes linked to poor stress management,
In stressful situations release of adrenaline causes arteries and
arterioles to constrict resulting in raised b.p.
Alcohol
Heavy drinkers far greater risk of heart disease.
Heavy drinking raises b.p and can cause irregular heartbeat
Excess alcohol consumption can result in direct tissue damage
including damage to the liver brain and heart.
Such damage contributes increased risk of CVD.
High level of alcohol can damage liver cells.
Impairs the ability of liver to remove glucose and lipids from the blood.
In liver alcohol is converted to ethanol. A three carbon carbohydrate.
Some may end up in VLDls increasing risk of plaque deposition.
Lungs allow rapid gas exchange between the atmosphere and the
blood.
Air drawn into lungs via trachea due to low pressure created by the
movement of the ribs and diaphragm.
Trachea divides into two bronchi which carry air to and from the lung.
Within each lung tree- like system of tubules ending in narrow tubes
bronchioles attached to alveoli which are the site of gas exchange.
Nothing unusual about having layer of mucus in tubes of gas exchange
system
Thin coating of mucus in tubes produced by goblet cells. Any dust or
debris that enter airways become trapped in the mucus.
Continually removed by cilia that cover the epithelial cells lining tubes
of the gas exchange system.
However those who have CF have mucus that is drier resulting in sticky
mucus that cilia can find more difficult to move.
Sticky mucus increases the chance of lung infection and makes gas
exchange less efficient.
How sticky mucus increases the chance of lung infections
Microorganisms become trapped in the mucus in the lungs.
Some of these can cause illness they are pathogens.
Mucus normally moved by cilia into back of mouth cavity where it is
either coughed or swallowed reducing risk of infection. Acid in stomach
kills most microorganisms that are swallowed.
With CF the mucus layer so sticky cilia cannot move mucus
Mucus production still continues as it would in normal lung and airways
build up with thickened mucus.
Low levels of oxygen in mucus, partly because oxygen diffuses slowly
through it and partly because epithelial cells use up more in CF
patients.
Harmful bacteria thrive in anaerobic conditions.
W.b.c fight infection within the mucus but as they break down,
releasing DNA which makes the mucus even stickier.
Repeated infections can eventually weaken the bodys ability to fight
the pathogens and cause damage to the structures of the gas
exchange system.
How sticky mucus reduces gas exchange
Gases such as oxygen cross the walls of the alveoli into the blood
system by diffusion.
Living organisms have to exchange substances with their surroundings.
E.g. they take in oxygen and nutrients and get rid of waste materials
such as CO2.
In unicellular organisms whole cell surface membrane is the exchange
surface.
Substances that diffuse in or out of a cell move down a concentration
gradient.
Gradient maintained by cell continuously using the substances
absorbed and producing waste.
Larger an organism more exchange has to take place to meet the
organisms needs.
Larger multicellular organisms have more problems absorbing
substances because of size of organisms surface area compared with
its volume.
This is known as the surface area to volume ratio, calculated by
dividing an organisms total surface area by its volume.
As organisms get larger s.a per unit of volume gets less. If larger
organisms relied on body surface for exchange of substances they
could not survive as exchange would be too slow.
Relying on outer body surface for gas exchange is only possible in
organisms with a very small volume or in large organisms that have a
high enough surface area to volume ratio.
Larger organisms have variety of special organs that increase the s.a
for exchange, increasing the s.a to volume ratio. Eg. Lungs provide a
large surface for gas exchange in mammals while minimising heat and
water loss form the moist surface.
Gas exchange surfaces
Within lungs alveoli provide large surface are for exchange of gases
between air and blood.
Large surface area of the alveoli, numerous capillaries around the
alveoli, thin walls of the alveoli and capillaries meaning a short
distance between the alveolar air and the blood in the capillaries.
Bodys demand for oxygen is enormous, diffusion across alveolar wall
needs to be fast.
Rate of diffusion dependent on three properties of gas exchange
surfaces
Surface area rate of diffusion is directly proportional to s.a. As the s.a
increases the rate of diffusion increases.
Concentration gradient. Rate of diffusion is directly proportional to
difference in concentration across the gas exchange surface. Greater
the concentration gradient faster the diffusion.
Thickness of gas exchange surface- rate of diffusion is inversely
proportional to thickness of the gas exchange surface. Thicker the
surface are slower diffusion.
Known as Ficks law
Large surface are of the alveoli, steep concentration gradient between
the alveolar air and the blood and the thin walls of the alveoli and the
capillaries combine to ensure rapid diffusion across the gas exchange
surface.
How sticky mucus might affect gas exchange
Sticky mucus layer in the bronchioles of person with CF tends to block
narrow airways, preventing ventilation of the alveoli below the
blockage.
Reduces number of alveoli providing surface are for gas exchange.
Blockages are more likely at the narrow ends of the airways.
These blockages often allow air to pass when person breathes in but
not when they breathe out, resulting in over- inflation of the lung tissue
beyond the blockage.
This can damage the elasticity of the lungs.
Those amino acids that animals have to obtain in their diet are known
as essential amino acids.
All amino acids contain an amine group, a carboxylic acid group and a
hydrogen attached to the central carbon atom.
Each type of amino acid has a different side chain called an R group.
Primary structure
2 amino acids join in a condensation reaction to form a dipeptide with a
peptide bond forming between the two subunits.
This process can be repeated to form polypeptide chains which may
contain thousands of amino acid.
A protein is made up of one or more polypeptide chain.
Sequence of amino acids in the polypeptide chains is known as the
primary structure of a protein.
Secondary structure
Chain of amino acid may twist to form an alpha helix. Within the helix
the hydrogen bonds form between the C=O of the carboxylic acid and
the NH of the amine group of different amino acids stabilising the
shape.
Several chains may link together with hydrogen bonds holding the
parallel chains in an arrangement known as the beta pleated sheet.
Within one protein molecule there may be sections with alpha helices
and other sections that contain beta pleated sheets.
Tertiary and quaternary structure
A polypeptide chain often bends and folds to produce a precise 3d
shape.
Chemical bonds and hydrophobic interactions maintain this final
tertiary structure of the protein.
An R group is polar when sharing of electrons within it is not quite
even.
Polar R groups attract other molecules, like water and are therefore
hydrophilic. The non-polar groups are hydrophobic.
Non-polar hydrophobic R groups are arranged so they face the inside of
the protein excluding water from the centre of the molecule.
A protein may be made up of several polypeptide chains held together.
For example haemoglobin, the protein found in r.b.c that carries oxygen
is made up of four polypeptide chains held together in a structure
known as the quaternary structure.
Only proteins with several polypeptide chains have a quaternary
structure; single chain proteins stop at the tertiary level
Conjugated proteins
Some proteins are known as conjugated proteins- they have another
chemical group associated with their polypeptide chains.
Globular and fibrous proteins