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4. Added several drops of 0.1M BaCL solution till white precipitate appears.
5. Observed and recorded observations
D. Phosphorus
1. Prepped 5 drops of the second portion of the fusion mixture into a micro test tube
2. Added 2-3 drops of Conc. HNO until the substance is acidic
3. Added 3 drops of Ammonium Molybdate solution
4. Heated nearly to boiling
5. Observed and recorded observations
Result
ELEMENT
Carbon, Hydrogen &
Oxygen
Nitrogen
Sulfur
Phosphorus
DESCRIPTION OF RESULT
Black precipitate
CHEMICAL EQUATION
Casein + O2 C + H2O
Discussion
The presence of carbon, oxygen and hydrogen was tested by heating casein,
a protein found mostly in milk products. The casein was heated for three minutes.
While heating, you can see the charring of casein, creating a black precipitate, this
means that carbon is present. There was also moisture in the test tube, this means
that hydrogen and oxygen is present.
The presence of nitrogen was tested in casein through mixture with soda
lime. It was then heated. A red litmus paper was placed on the mouth of the test
tube while heating. There was also gas inside the test tube while heating. The red
litmus paper turned to blue, this is due to NH 3 gas. The bad odor indicates the
presence of nitrogen.
Fusion was used to oxidize sulfur to sulfate and phosphorus to phosphate.
Casein was mixed with the fusion mixture and was heated in a porcelain crucible.
The substance first turned black and then to white. It was dissolved in a hot water
and filtered. This was then used in the test for sulfur and phosphorus.
The presence of sulfur was tested by using the filtrate from the fusion method
and HCl. It took 17 drops of HCl until it was made acidic. It heated to boiling. BaCl 2
was the added. A white precipitate was then formed. This is the BaSO 4 or barium
sulfate.
The presence of phosphorus was tested by using the filtrate from the fusion
method and nitric acid. Nitric acid was added until acidic. After that, ammonium
molybdate was added then heated. A yellow precipitate was formed. This is the
ammonium phospho-molybdate.
Conclusion
The experiment confirmed that carbon, hydrogen, oxygen, nitrogen, sulfur
and phosphorUs is present in proteins.
Reference
1. https://mtfiles.wordpress.com/biochemistry/elementary-composition-ofproteins/
2. https://mynotesarchive.wordpress.com/2012/02/18/chem-lab-notes/