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Introduction
Mitochondria are intricate highly organized cellular organelles, which play crucial
roles not only in energy homeostasis but also in many signalling, biosynthetic
and cell death pathways. Furthermore, mitochondria are extremely dynamic
organelles that constantly divide and combine and travel inside the cell. For
these reasons, it is not surprising that a broad range of procedural approaches
have been developed to gauge mitochondrial activities.
This review aims to discuss the major cytochemical, biochemical, and molecular
biological techniques used to inspect mitochondrial dynamics with a focus on the
more recently developed technologies.
Fluorescence
maximum (nm)
Excitation
Emission
3,3 Dihexyloxacarbocyanin
iodide (DiOC 6 )
5,5 ,6,6 -Tetrachloro-1,1 ,3,3
tetraethylbenzimidazolylcarboc
yanine
iodide (JC-1)
MitoTracker
MitoFluor
488
501
514
495
507
522
529
Tetramethylrhodamine ethyl
ester (TMRE)
Tetramethylrhodamine methyl
ester (TMRM)
549
574
548
573
References
1. Analysis of Mitochondrial Dynamics and Functions Using Imaging
Approaches, Kasturi Mitra and Jennifer Lippincott-Schwartz
2. Fluorescent probes for super-resolution imaging in living cells, Marta
Fernndez-Surez and Alice Y. Ting. Nature Reviews Molecular Cell Biology 9, 929943 (December 2008)