ANALISIS KANDUNGAN -KAROTEN PADA BEBERAPA VARIETAS CABAI MERAH

(CAPSICUM ANNUUM) SECARA SPEKTROFOTOMETRI UV-VIS

Harti widiastuti
Fakultas Farmasi Universitas Muslim Indonesia
ABSTRACT
A have applied of research about content of -carotene in three quality of cabai
namely cabai merah besar (Capsicum annuum L. Var. Abreviata Einger huth), cabai merah
keriting (Capsicum annuum L. Var. Longum sendt) and cabai rawit (Capsicum frustescens
L). For to Analysis and senelles content -carotene in cabai. Cabai sample to be extraction
in demarcation by acetone. The result of extraction to extract again with petroleum eter and
applied safonification with KOH 15%. Qualitative analysis applied with Chromatography
method in thin layer using petroleum eter : Benzen (9:1).The result it showing there are spot
which same on three samples and -carotene pure as the compere. While Quantitative
analysis with spectrofotometri visible using -carotene pure as compere showing that the
content of -carotene cabai merah besar 154,32 mg/g, cabai merah keriting 181,41 mg/g
and cabai rawit 146,85 mg/g.

UJI SENSITIVITAS BEBERAPA ANTIBIOTIKA PADA JAMUR PENYEBAB KANDIDIASIS

MULUT
Hendra Herman
Fakultas farmasi Universitas Muslim Indonesia
ABSTRACT
Has been done research Sensitivity Test of Several Antibiotic on Candidiasis Mouth
Caused by mushroom. Kirby Bouer agar diffuse method used in this research, in which 15
specimen of acne were taken and performed three times replication to get a better result.
Result of the research based on diameter of inhibitory zone showed that from three
replication the antibiotic which sensitivity the most on
Candidiasis mouth cause
mushroom are Nystatin (60%), Griseofulvin (33,33%), Ketokenazol (26,67%), Flukonazol
(26,67%) , Myconazol (6,67 %). The conclusion is antibiotic which sensitivity the most on
Candidiasis mouth caused by mushroom is Nystatin.
Key Words : Sensitivity Test, Antibiotic, Candidiasis Mouth.

PEMERIKSAAN FARMAKOGNOSTIK DAN PROFIL KROMATOGRAFI LAPIS TIPIS

TUMBUHAN BAGORE (Caesalpinia crista Linn) ASAL DAERAH POLEWALI
MANDAR SULAWESI BARAT
Risda Waris
Fakultas farmasi Universitas Muslim Indonesia
ABSTRACT
Pharmacognostic assay and using thin layer chromatography of Bagore plant
(Caesalpinia crista, Linn).The morphology showed Bagore was the dycotiledonae with
woody bark. Transverse section leaves indicated there are stomata anisositik. Transverse
section of bark showed open kolateral. Physic measurement was including total ash level
measurement 6,24 % and undissolved acid ass level equal to 0,55 %. Ekstraction level
measurement which included dissolved water extraction level 73,69 % and dissolved etanol
ectraction level equals 36,24 %. It was countaining steroid compounds. Thin layer
chromatography result with specific reagent that is on Lieberman-Bouchard reagent after
sprayed showed violet this showed positifely countaining steroid compound group
Key word : Bagore (Caesalpinia crista Linn), identification, juice content, ash level.

STABILITAS DAN MASA SIMPAN SEDIAAN SUSPENSI KERING ERITROMISIN

ETILSUKSINAT DENGAN VARIASI BAHAN PENSUSPENSI
Faradiba* , Amran Ilyas Tandjung**, NurFadillah*
*Fakultas Farmasi Universitas Muslim Indonesia
** Fakultas Farmasi Universitas Hasanuddin
e-mail : Faradiba76@yahoo.com
ABSTRACT
An Investigation on how to know the chemist stability and the expired date on dry
suspension formulation of erythromycin ethylsuccinate has been done in variation of
suspending agents. Evaluation of Chemical stability has been done by inspection a rate of
eritromisin degradation with accelerated stability test by stored sample on temperature
above room temperature (250C) that is 30, 40, 50 and 600C during 4 days and decided the
remain of the erythromicyn concentration each 1 day (24 hours). The Results is rate of
erythromycin degradation in stored by normal temperature (25 0C) for the formula I, II and III
to follow that is 0,00043 day-1; 0,00026 day-1 and 0,00064 day-1. Th stored time for the
formula I, II and III to follow that is 0,7; 1,13; and 0,5 years. The result have been shown that
formula II (Gum xanthan as a suspending agent) was the most stable dry suspension
chemically.
Key Words : Stability , Expired Date, Dry Suspension , Erythromycin Ethylsuccinate ,
Suspending Agent.

VALIDASI METODE PENETAPAN KADAR GABAPENTIN TERDERIVATISASI SECARA

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY DAN APLIKASINYA DALAM
SEDIAAN KAPSUL
Nurmaya Effendi
Fakultas Farmasi, Universitas Gadjah Mada
ABSTRACT
Gabapentine, is a new antiepileptic drug which has a structural analogue of
neurotransmitter -aminobutyric acid (GABA). Gabapentine inhibits calcium uptake of gated
calcium channels presinaps in the brain. This research aimed to develop and validate a new
HPLC method with UV detection for determination of gabapentine in capsule. The method is
based on the reaction of gabapentine with 1-fluoro-2,4-dinitrobenzen (FDNB) in acetonitrile
which carried out in the presence of borate buffer (pH = 9,5) at 65 oC for 20 minutes, which
formed a yellow colored product with the absorption at 367 nm. The product was separated
by HPLC using A Zorbax ODS (Agilent) column (250 x 4.6 mm, 5 m) was utilized as
stationary phase and 50 mM acetate buffer (pH 3.7) : methanol : acetonitril (25 : 30 : 45
v/v/v) as mobile phase, with flow rate 0.8 mL/min, and the detection at 367 nm. The
purposed method was validated by means of accuracy, precision, linearity, selectivity, LOD
and LOQ, and the complied with the requirement for validity. The method has been
succesfully applied for the determination of gabapentine in capsule with LOD = 1.05 g/mL
and LOQ = 3.49, linear quantitative response curve was generated over a concentration
range of 3.49 - 100 g/mL with the correlation coefficient of 0.99996 and recovery range of
97.27 104.24% with RSD 2.63%.
Keywords : Gabapentin, Fdnb, Hplc, Validation.

PENGARUH BAHAN PENSUSPENSI TERHADAP AKTIVITAS PENGAWET METIL

PARABEN
A.Trihadi Kusuma
Fakultas Farmasi Universitas Muslim Indonesia Makassar
ABSTRACT
AnalyzeQuality Of Cooking Oil With Double Fractination In Tidiness With The Recuring
Usage. Have been research concerning quality of cooking oil double fractination in tidiness
with the recuring usage by aims to determine quality of cooking oil with double fractination in
tidiness with the recuring usage. The parameter of quality test of oil and fat is free fat acid
number, saponification number, peroxide number, iodium number and water content with
using titrimetri method. Kind of cooking oil which used were cooking oil double fractination.
Research result show that cooking oil which used repeatedly with 3 times intensity has
reduce of quality and no safe to consumed based on what standardized by National
Standarization Agency.
Key Words: methyl paraben, suspending agent,preservatif efectivity.

FORMULASI SEDIAAN TETES MATA DENGAN ZAT AKTIF KOMBINASI

KLORAMFENIKOL DAN ZINK SULFAT
Zainal Abidin
Fakultas Farmasi Universitas Muslim Indonesia
ABSTRACT
A research has been done to formulate eye drop with chloramphenicol and zinc sulfat
as active ingredient. The planned formulation contain chloramphenilcol 0,25%, zink sulfat
0,25%, boric acid 1,9%, methylparaben 0,1%, and water steril until 100 %. To still keep
stability of eye drop so added additional ingredient such as Boric acid as buffer,
methylparaben as preservative, and water steril as carrier. Result of the research that purity
concentration of chloramphenicol in eye drop is 98,62% and zink sulfat is 108,2% as active
ingredient approriate with standart of indonesia,s Pharmacope fourth edition. Sterility test
shown that eye drop chloramphenicol and zink sulfat there is not found microbe or free from
microbe aerob, anaerob, and mold.
Key words : Eye drop, Chloramphenicol, Zink sulfat

IDENTIFIKASI MAKROSKOPIK DAN MIKROSKOPIK SEDIAAN JAMU ASAM URAT

YANG BEREDAR DI KOTA MAKASSAR
Virsa Handayani
Fakultas Farmasi Universitas Muslim Indonesia, Makassar
Email : virsaichafarmasi@yahoo.com
ABSTRACT
Was done research about pharmacognostic identification from muscle acid medicinal
herbs that circulating at Makassar city., with aim to get pharmacognostics data from
medicinal herbs of muscle acid that circulating at Makassar, for security in consuming. This
research covers macroscopic and microscopic. On microscopic test medicinal herbs powder
A, B dan C there are endosperm, parenkim with sekresi cell, fiber and calsium oksalat. From
both offabove sample,really contains Zingiberis Rhizoma, Phaleria macrocarpa andyy `Piper
nigrum
Key word : Makroskopic, mikroskopic, muscle acid medicinal herbs.

UJI AKTIVITAS ANTIMIKROBA EKSTRAK DIETIL ETER DAUN GAMAL (Glyricidia

sepium (JACQ.) STEUD.) SECARA KLT-BIOAUTOGRAFI
A.Mumtihanah Mursyid
Fakultas Farmasi Universitas Muslim Indonesia
ABSTRACT
Studies have been conducted testing the antimicrobial activity of leaf extracts gamal
(Glyricidia sepium (Jacq.) Steud.). Preliminary research conducted by the screening test
using the test microbe Staphylococcus aureus, Salmonella typhi, Streptococcus mutans,
Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli, Vibrio sp, Bacillus
subtilis and Candida albicans to extract ethanol, diethyl ether and n-butanol from leaf gamal
(Glyricidia sepium (Jacq.) Steud.) with levels of 1 mg / ml. The results showed that the
diethyl ether extract active has hampered the growth of microbes Staphylococcus aureus,
Escherichia coli, Streptococcus mutans, Salmonella typhi, and Pseudomonas
aeruginosa.Tests for antimicrobial activity against diethyl ether extracts of leaves gamal
(Glyricidia sepium (Jack). Steud.) Carried out by liquid dilution method to determine the
value of MIC at a concentration of 0.025%, 0.05%, 0.1%, 0.2%, 0, 4%, 0.8% and 1.6%,
followed by etching in a solid medium to determine the price of teaching. The results showed
that the MIC could not be determined due to turbidity test solution, while the price KBM
0.05% for Escherichia coli, Salmonella typhi 0.4%, 0.8% for Pseudomonas aeruginosa and
Streptococcus mutans and 1.6% for Staphylococcus aureus,To estimate the class of
compounds that provide antimicrobial activity test carried out TLC-Bioautografi. Obtained the
best results from the separation of compounds by TLC using the eluent n-hexan: ethyl
acetate (2:1). TLC-Bioautografi test results showed that the spots with Rf 0.27 and Rf value
of 0.363 provides antimicrobial activity against Escherichia coli, Rf 0.363 against
Pseudomonas aeruginosa, and Rf 0.45 against Staphylococcus aureus, Salmonella typhi,
and Streptococcus mutans. The results of identification of chemical components showed that
the active compounds gave positive results in spotting penampak phenol group at Rf 0.27
and Rf 0.36 and Rf 0.45 on steroids.
Key word: antimicrobial activity, screening, TLC-Bioautografi, Gamal (Glyricidia sepium
(Jack).Steud.)

PRODUKSI YOGHURT dan SOYGHURT DARI SUSU SKIM dan SUSU KEDELAI
DENGAN VARIASI BAHAN PENSTABIL
Rachmat Kosman
Fakultas Farmasi Universitas Muslim Indonesia Makassar
ABSTRACT
A research had been done of Yoghurt and soyghurt production from skim milk and
soya milk with variation of stabilizer agent. Yoghurt is fermentation milk product made from
fermentation milk uses acid lactic bacteria, soyghurt is fermentation milk product made from
fermentation soya milk uses acid lactic bacteria. To get the best quality of yoghurt and
soyghurt usually added stabilizer agent so that get the best consistency, viscosity and
stability. The aim of this investigation is to determine influence of milk kinds and stabilizer
agent at Yoghurt and soyghurt production from skim milk and soya milk. Yoghurt made by
skim milk and soyghurt made by soya milk with three variation of stabilizer agent, which
0,9% gelatine, 0,9% CMC, and 0.9% caragenan. The assay were organoleptic observation,
pH and viscosity measurement, % acid lactic content test, and total of acid lactic bacteria

test. The assay is done after storage at 5 0C. Organoleptic observation is consistency, odor,
and taste. pH measurement by pHs universe paper than viscosity measurement by
brookfield viscometer with three replication, 50 rpm rates. % lactat acid content test by
alkalimetry method. And total of acid lactid bacteria test by pouring method. The investigaton
result can be concluded that stabilizer agent influence consistency, viscosity and prevent of
separation between solid and liquid phase at yoghurt and soyghurt. 0,9% gelatine is the best
stabilizer agent at yoghurt and soyghurt prodution.
Key Words :Yoghurt, Soyghurt, Skim, Stabilizer Agent