RESULTS:Data Sheet showing the enzymatic activity in Sweet potato extract, Irish potato extract and

milk obtained in the lab.

A. Polyphenol Oxidase (Phenolase) Sweet Potato

Reaction

Observations

Inferences

CAFFEIC ACID-(Enzyme)
Colour of substrate
retained;colourless.
Substrate and Water
Colour of enzyme retained;cream.

Enzyme and Water

Color change from colourless to
yellow.

Enzyme and Substrate

Colour change from clourless to

light orange.

Enzyme, Inhibitor and

Substrate

Due to the presence of the substrate

there werent no enzyme present to
catalyse the rate of reaction
therefore there was no colour
change.
Due to the presence of the enzyme
being prevalent over that of the
substrate which wasnt present to
catalyse the reaction moverover,
there was a colour change express
in the enzyme which was a cream
colour.
The enzymes catalyzes the
chemical reaction involving the
substrate in this case of a single
substrate present, the substrate
bonds with the enzymes active site,
which transpire to produce a
enzyme-substrate complex which
changed the colour from colourless
to yellow.
The enzymes catalyzes the
chemical reaction involving the
substrate in this case of a single
substrate present, the substrate
bonds with the enzymes active site,
which transpire to produce an
enzyme-substrate complex which
changed the colour from colourless
toa light orange.

CATECOL-(Enzyme)
Substrate and Water

Colour of substrate
retained;colourless.

Colour of substrate
retained;colourless.

Enzyme and Water

Colour of enzyme retained;cream.

Colour of enzyme retained;cream.

Colour change from clourless to

light orange.
No colour change,solution retained
colour of enzyme(cream).

Colour change from clourless to

light orange.
No colour change,solution retained
colour of enzyme(cream).

Substrate and Water

Colour of substrate
retained;colourless.

Colour of substrate
retained;colourless.

Enzyme and Water

Colour of enzyme retained;cream.

Colour of enzyme retained;cream.

Colour of enzyme retained;cream.

Colour of enzyme retained;cream.

Colour of enzyme retained;cream.

Colour of enzyme retained;cream.

Colour of substrate retained;yellow.

Colour of substrate retained;yellow.

Colour of enzyme retained;cream.

Colour of enzyme retained;cream.

Colour of substrate retained;light

yellow.

Colour of substrate retained;light

yellow.

Enzyme colour retained (cream).

Colour of enzyme retained;cream.

Enzyme, Inhibitor and

Substrate
Enzyme, Inhibitor and
Substrate
GUAICOL-(Enzyme)

Enzyme and Substrate

Enzyme, Inhibitor and
Substrate
PYRAGALLOL-(Enzyme)
Substrate and Water
Enzyme and Water
Enzyme and Substrate
Enzyme, Inhibitor and
Substrate
TYROSINE-(Enzyme)
Substrate and Water
Enzyme and Water

Colour of enzyme retained;cream.

Coloured of substrate
retained;colourless.
Colour of enzyme retained;cream.

Colour of substrate retained;yellow.

Retention of enzyme colour;cream.

Colour of enzyme retained;cream.

Retention of enzyme colour;cream.

Colour of substrate retained;light

yellow.

Enzyme and Substrate

Enzyme, Inhibitor and
Substrate

B. Polyphenol Oxidase (Phenolase) Irish Potato

Reaction

Observations

Inferences

CAFFEIC ACID-(Enzyme)
Substrate and Water

Enzyme and Water

Enzyme and Substrate
Enzyme, Inhibitor and
Substrate

Colour of substrate
retained;colourless.
Colour of enzyme retained;cloudy.

Colour of substrate
retained;colourless.
Colour of enzyme retained;cream.

The subtrate colour retained;cream

then changed to a pale yellow
colour upon the addition of the
enzyme.
Colour of substrate
retained;colourless.No reaction
occurred.

Color change from colourless to

yellow.
Colour change from clourless to
light orange.

CATECOL-(Enzyme)
Substrate and Water

Enzyme and Water

Enzyme and Substrate

Enzyme, Inhibitor and

Substrate

Colour of substrate
retained;colourless.

Colour of substrate
retained;colourless.

Colour of enzyme retained;cloudy.

Colour of enzyme retained;cream.
The subtrate colour retained;cream
then changed to a pale peach
colour upon the addition of the
enzyme.
Colour of substrate retained;cream
colour.No further colour change
occurred

Colour change from clourless to

light orange.
No colour change,solution retained
colour of enzyme(cream).

GUAICOL-(Enzyme)
Substrate and Water

Colour of substrate
retained;colourless.

Colour of substrate
retained;colourless.

Enzyme and Water

Colour of enzyme retained;cloudy.

Colour of enzyme retained;cream.

The subtrate colour retained;cream

then changed to a pale peach
colour upon the addition of the
enzyme.
Colour of substrate
retained;colourless .No further

Colour of enzyme retained;cream.

Enzyme and Substrate

Enzyme, Inhibitor and

Substrate

Colour of enzyme retained;cream.

reaction or colour change

occurred.
PYRAGALLOL-(Enzyme)
Substrate and Water
Enzyme and Water

Enzyme and Substrate

Enzyme, Inhibitor and

Substrate

Colour of substrate
retained;colourless.
Colour of enzyme
retained;colourless.No colour
change occurred.
The subtrate colour retained;cream
then changed to bright yellow
colour upon the addition of the
enzyme.
The subtrate colour retained;cream
then changed to a pale yellow
colour upon the addition of the
enzyme.

TYROSINE-(Enzyme)
Substrate and Water

Enzyme and Water

Enzyme and Substrate

Enzyme, Inhibitor and

Substrate

Colour of substrate
retained;yellow.
Colour of enzyme retained;cream.

Colour of substrate retained;light

yellow.

Colour of enzyme retained;cream.

Colour of enzyme retained;cream.

Colour of substrate
retained;colourless.
Colour of enzyme
retained;colourless.No colour
change occurred.
The subtrate colour retained;cream
then changed to light brown colour
upon the addition of the enzyme.
Colour of substrate
retained;colourless .No further
reaction or colour change
occurred.

Colour of substrate
retained;yellow.
Colour of enzyme retained;cream.

Colour of substrate retained;light

yellow.

C. Peroxidase Sweeet Potato

Reaction

Observations

Inferences

CAFFEIC ACID-(Enzyme)
Substrate and Water
Enzyme and Water
Enzyme and Substrate
Enzyme, Inhibitor and
Substrate
CATECOL-(Enzyme)
Substrate and Water
Enzyme and Water
Enzyme and Substrate
Enzyme, Inhibitor and
Substrate

No colour change occurred.

Colour of substrate
retained;colourless.

Colour of enzyme retained;cloudy.

Colour of enzyme retained;cream.
Turned dark yellow upon addition of
the enzyme.
The subtrate colour retained;yellow
until the addition of the enzyme.
No colour change occurred.

Color change from colourless to

yellow.
Colour change from clourless to
light orange.
Colour of substrate
retained;colourless.

Turned light yellow upon addition of

the enzyme.
Colour of enzyme retained;cream.
Intial colour;white turned brown
upon addition of enzyme.
Colour change from clourless to
light orange.
The substrate colour retained;clear
No colour change,solution retained
until the addition of the enyme to a
colour of enzyme(cream).
cloudy precipitate.

GUAICOL-(Enzyme)
No colour change occurred.

Colour of substrate
retained;colourless.

Enzyme and Water

Colour of enzyme retained;clear.

Colour of enzyme retained;cream.

Enzyme and Substrate

Intial colour:cloudy turned yellow

upon addition of enzyme.

Colour of enzyme retained;cream.

Enzyme, Inhibitor and

Substrate

The substrate colour retained;clear

until the addition of the enzyme to a
cloudy precipitate.

Colour of enzyme retained;cream.

No colour change occurred.

Colour of substrate retained;yellow.

Colour of enzyme retained;cloudy.

Colour of enzyme retained;cream.

Substrate and Water

PYRAGALLOL-(Enzyme)
Substrate and Water

Enzyme and Water

Enzyme and Substrate
Enzyme, Inhibitor and
Substrate
TYROSINE-(Enzyme)
Substrate and Water
Enzyme and Water
Enzyme and Substrate
Enzyme, Inhibitor and
Substrate

The subtrate colour retained;yellow

with a hint of green upon the
addition of the enzyme.
The subtrate colour retained; pale
yellow upon the addition of the
enzyme.

Colour of substrate retained;light

yellow.
Colour of enzyme retained;cream.

Colour of enzyme retained;cream.

No colour change occurred.
No colour change occurred.

Colour of substrate retained;yellow.

The subtrate colour retained;clear

with a hint of gray upon the addition
of the enzyme.
The subtrate colour retained; clear
yellow to a cloudy precipitate upon
the addition of the enzyme.

Colour of enzyme retained;cream.

Colour of substrate retained;light

yellow.

D. Peroxidase Irish Potato

Reaction

Observations

Inferences

CAFFEIC ACID-(Enzyme)
Substrate and Water
Enzyme and Water
Enzyme and Substrate

Colour of substrate
retained;colourless.
Colour of enzyme retained;cloudy.

Colour of substrate
retained;colourless.
Colour of enzyme
retained;cream.

The subtrate colour

retained;colourless to yellow colour
upon the addition of the enzyme.
The subtrate colour retained; yellow
colour upon the addition of the
enzyme.

Color change from

colourless to yellow.
Colour change from
clourless to light orange.

Substrate and Water

Colour of substrate
retained;colourless.

Colour of substrate
retained;colourless.

Enzyme and Water

Colour of enzyme retained;cloudy.It

took the colour of the enzyme,
efferevence occurred.

Colour of enzyme
retained;cream.

Enzyme, Inhibitor and

Substrate

CATECOL-(Enzyme)

Enzyme and Substrate

Enzyme, Inhibitor and

Substrate

GUAICOL-(Enzyme)
Substrate and Water

Enzyme and Water

Enzyme and Substrate

Enzyme, Inhibitor and

Substrate

The subtrate colour retained;mauve

upon the addition of the enzyme.
The subtrate colour retained;
colourless to a mauve/redish brown
colour upon the addition of the
enzyme.

Colour change from

clourless to light orange.
No colour change,solution
retained colour of
enzyme(cream).

Colour of substrate
retained;colourless.

Colour of substrate
retained;colourless.

Colour of enzyme retained;cloudy.It

took the colour of the enzyme,
efferevence occurred.

Colour of enzyme
retained;cream.

The subtrate colour retained;peach

to an orange colour upon the
addition of the enzyme.
The subtrate colour retained;
colourless to a peach colour
followed by an orange precipitate
upon the addition of the enzyme.

Colour of enzyme
retained;cream.

Colour of substrate
retained;colourless.
Colour of enzyme retained;cloudy.It
took the colour of the enzyme,
efferevence occurred.

Colour of substrate
retained;yellow.
Colour of enzyme
retained;cream.

The subtrate colour

retained;colourless to an dark yellow
colour upon the addition of the
enzyme.
The subtrate colour retained;
colourless to a deep yellow colour
upon the addition of the enzyme.

Colour of substrate
retained;light yellow.

Colour of enzyme
retained;cream.

PYRAGALLOL-(Enzyme)
Substrate and Water

Enzyme and Water

Enzyme and Substrate

Enzyme, Inhibitor and

Substrate
TYROSINE

Colour of enzyme
retained;cream.

Colour of enzyme
retained;cream.
Colour of substrate
retained;colourless.

Substrate and Water

Colour of enzyme retained;cloudy.It

took the colour of the enzyme,
efferevence occurred.

Colour of substrate
retained;yellow.

The subtrate colour

retained;colourless to a peach colour
upon the addition of the enzyme.
The subtrate colour retained;
colourless with effervescence upon
the addition of the enzyme.

Colour of enzyme
retained;cream.

Enzyme and Water

Enzyme and Substrate

Enzyme, Inhibitor and

Substrate

E. Xanthine Dehydrogenase

1. Substrate Concentration
0.0M

0 min

0.2M

26 min

0.4M

30.5 min

0.6M

46 min

0.8M

58 min

1.0M

105.5min

2. Heat Treatment
Tube A

> 60 min

Tube B

60 min

Tube C

>60 min

Colour of substrate
retained;light yellow.