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Chemistry
Food Chemistry 101 (2007) 236240
www.elsevier.com/locate/foodchem
Nucleo de Pesquisa de Produtos Naturais, CCS, Bloco H, Universidade Federal do Rio de Janeiro, Ilha do Fundao, 21941-590, Rio de Janeiro, Brazil
b
EMBRAPA-RJ, Avenida das Americas 29501, Guaratiba, 23020-470, Rio de Janeiro, Brazil
c
IMPPG/CCS/UFRJ Bloco I, Ilha do Fundao, 21941-590, Rio de Janeiro, Brazil
Departamento de Produtos Naturais e Alimentos, Faculdade de Farmacia UFRJ, Centro de Ciencias da Saude, Bloco A 2o andar, Universidade Federal
do Rio de Janeiro, Ilha do Fundao, 21941-590, Rio de Janeiro, Brazil
Received 21 March 2005; received in revised form 16 January 2006; accepted 18 January 2006
Abstract
Lippia origanoides H.B.K. (Verbenaceae) is a plant known in Oriximina (Brazil) as Salva-de-Marajo. Its leaves are widely used as of
a spice in cooking and in traditional medicine. The chemical composition of the essential oil obtained from its leaves, analyzed by GC
and GC/MS, showed a high content of oxygenated monoterpenes (66.0%), carvacrol (38.6%) and thymol (18.5%) being the major constituents. Considering that previous studies on the same plant species showed carvacrol as a trace or absent compound, we propose the
existence of a new chemotype for this species. A high carvacrol content in the essential oil determines the plants suitability for the preparation of oregano condiment. The antimicrobial activity of this essential oil was determined by the drop diusion method, showing
highly signicant inhibition zones for all microorganisms tested.
2006 Elsevier Ltd. All rights reserved.
Keywords: Lippia origanoides; Essential oil; Oregano; Antimicrobial activity; Thymol; Carvacrol
1. Introduction
Lippia origanoides H.B.K. (Verbenaceae) is a slender,
very aromatic shrub or tree, up to 3 m tall (Jansen-Jacobs,
1988; Moldenke, 1965). The species is native to some countries of Central America (Mexico, Guatemala, Cuba) and
northern South America, especially in the Amazon Region
(Guiana, Venezuela, Brazil and Colombia) (Maisch, 1885;
Pascual, Slowing, Carretero, Mata, & Villar, 2001). This
plant is popularly known in the North of Brazil by the names
of Salva-de-Marajo and Alecrim-dAngola; in Para
State it is used for culinary and medicinal purposes (Oliveira,
2004). In Mexico, L. origanoides (syn. Lippia schomburgkiana Schauer) is called oregano, and the Mexican Pharmaco*
0308-8146/$ - see front matter 2006 Elsevier Ltd. All rights reserved.
doi:10.1016/j.foodchem.2006.01.022
237
238
Table 1
Qualitative and quantitative chemical composition of Lippia origanoides
essential oils
Compounds
RIcalc.
RIlit.
Identication
method
a-Thujene
a-Pinene
Camphene
Sabinene
1-Octen-3-ol
Myrcene
a-Phellandrene
d-3-Carene
a-Terpinene
q-Cymene
Limonene
1,8-Cineole
(Z)-b-Ocimene
(E)-b-Ocimene
c-Terpinene
(Z)-linalool oxide
Terpinolene
Linalool
Ipsdienol
Borneol
Umbellunone
Terpinen-4-ol
p-Cymen-8-ol
a-Terpineol
Thymyl methyl ether
Carvacryl methyl ether
Phenyl ethyl acetate
Thymol
Carvacrol
Thymyl acetate
Carvacryl acetate
Geranyl acetate
b-Elemene
(E)-caryophyllene
b-gurjunene
(E)-a-bergamotene
(Z)-b-farnesene
a-Humulene
(E)-b-Farnesene
allo-Aromadendrene
Germacrene D
b-Selinene
b-Bisabolene
c-Cadinene
d-Cadinene
c-Bisabolene
Germacrene B
Caryophyllene oxide
2-Phenyl ethyl tiglate
1-Epi-cubenol
Epi-a-cadinol
a-Muurolol
a-Cadinol
931
937
953
976
981
992
1006
1012
1019
1028
1032
1035
1040
1051
1062
1076
1090
1101
1150
1168
1177
1180
1187
1192
1237
1247
1258
1289
1303
1358
1375
1386
1392
1420
1430
1437
1445
1454
1459
1461
1481
1486
1509
1514
1524
1533
1557
1583
1588
1633
1643
1648
1656
931
939
953
976
978
991
1005
1011
1018
1026
1031
1033
1040
1050
1062
1074
1088
1098
1147
1165
1171
1177
1183
1189
1235
1244
1256
1290
1298
1355
1371
1383
1391
1418
1432
1436
1443
1454
1458
1461
1480
1485
1509
1513
1524
1533
1556
1581
1585
1627
1640
1645
1653
0.9
0.9
0.1
0.1
0.4
1.8
0.2
0.1
1.1
10.3
0.5
0.5
0.1
0.2
4.1
0.1
0.3
2.5
0.2
0.2
0.3
0.9
0.1
0.2
2.2
0.1
0.1
18.5
38.6
0.4
1.2
0.1
0.2
5.9
0.2
0.1
0.3
0.1
0.1
0.6
0.2
0.5
0.1
0.1
0.1
0.6
0.1
0.2
0.8
0.4
0.1
0.2
1.3
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
1,2
Monoterpene hydrocarbons
Oxygen containing monoterpenes
Sesquiterpene hydrocarbons
Oxygen containing sesquiterpenes
Other
Total identied
20.7
66.0
9.0
1.1
1.3
98.1
239
Table 2
Growth inhibition zones of microorganisms in millimetres
Microorganisms
Amphotericin B
Vancomycin
Methicillin
25
27
40
35
24
20
16
24
18
20
30
40
25
20
18
21
20
25
18
08
20
26
10
10
15
10
240