Professional Documents
Culture Documents
AND
JANET E. FOLEY1
ABSTRACT Ticks, eas, and vector-borne pathogens were surveyed in diverse small mammals in
Yosemite National Park, California, from 2005 to 2007. A total of 450 unique captures of small mammals
was collected during a 3-yr period and yielded 16 species of eas and 10 species of ticks, including
known vectors of Anaplasma phagocytophilum and Borrelia burgdorferi and plague. Serology was
performed for A. phagocytophilum, spotted fever group Rickettsia spp., B. burgdorferi, and Yersinia
pestis. A. phagocytophilum exposure was identied in 12.1% of all wild small mammals tested, with
seropositive animals in 10 species, notably Beldings ground squirrels (Spermophilus beldingi), jumping
mice (Zapus princeps), and voles (Microtus sp.). Spotted fever group Rickettsia spp. exposure was
detected in 13.9% of all small mammals tested, with seropositive animals in eight species. Additionally,
37.0% of rodents in ve species tested were seropositive for B. burgdorferi. No individuals were
seropositive for Y. pestis. No animals were polymerase chain reaction positive for any pathogen tested.
These results provide baseline data for future research and prediction of emerging vector-borne
disease in Yosemite National Park, as well as adding to the known ranges and host species for tick and
eas in California.
KEY WORDS Anaplasma phagocytophilum, Borrelia species, eas, plague, ticks
102
Table 1.
Site
Elevation
UTM (N)
UTM (E)
Foresta
Hodgdon Meadows
Crane Flats
Porcupine Creek
Tuolumne Meadows
Dana Meadows
1,200
1,200
1,800
2,400
2,800
3,000
374220
374748
374631
374803
375208
375414
1194516
1195151
1194750
1193437
1192121
1191504
0
0
0
0
0
0
0
0
0
0
1
0
1
0
0
0
0
0
2
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
1
1
0
0
0
0
0
2
0
0
0
0
0
0
1
0
0
0
1
0
0
0
0
0
0
0
2
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
1
0
1
0
1
0
2
0
6
0
0
0
0
4
0
0
0
0
0
14
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
1
1
0
2
0
0
0
0
1
3
0
0
0
0
0
0
0
0
0
7
Chaetodipus californicus (1)
Glaucomys sabrinus (8)
Homo sapiens (2)
Microtus californicus (2)
Microtus longicaudus (8)
Microtus montanus (26)
Peromyscus boylii (1)
Peromyscus maniculatus (137)
Peromyscus truei (6)
Sorex lyelli (4)
Sorex trowbridgii (1)
Spermophilus beecheyi (32)
Spermophilus beldingi (126)
Tamias alpinus (1)
Tamias minimus (1)
Tamias speciosus (7)
Thomomys monticola (5)
Zapus princeps (25)
Total
0
0
0
0
0
0
1
3
0
0
0
0
1
0
0
0
0
0
5
Ixodes
woodi
Ixodes
spinipalpis
Ixodes
sculptus
Ixodes
pacificus
Ixodes
kingi
Ixodes
hearli
Ixodes
angustus
Haemaphysalis
leporispalustris
Dermacentor
sp.
Dermacentor
occidentalis
Number of individuals of each small mammal species infested with various species of ticks from YNP, California
Table 2.
Results
Tick species
richness
103
1
1
1
1
0
1
2
4
1
0
2
1
7
0
0
0
0
0
22
January 2011
0
0
0
0
0
0
10
0
0
0
0
1
0
0
0
0
0
0
0
Callistopsyllus
terinus
0
0
0
1
0
0
6
0
0
0
0
0
0
0
0
0
0
0
0
Catallagia
s. sculleri
0
0
0
0
0
0
0
0
2
0
0
0
0
0
0
0
0
0
0
Corrodopsylla curuata
obtusata
0
0
0
0
0
0
0
0
0
0
0
2
0
4
0
0
0
0
0
Eumolpianus
eutamiadis
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
3
0
0
1
Foxella ignotus
recula
Opisodasys
vesperalis
0
2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
56
0
0
0
0
0
0
0
0
Oropsylla
montana
0
0
0
0
0
0
0
0
0
0
0
13
1
0
0
0
0
0
0
Oropsylla
t. tuberculata
0
0
0
0
0
0
13
0
0
0
0
1
0
0
0
0
0
0
0
Peromyscopsyllus hesperonomys
adelpha
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Peromyscopsylla
selenis
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
Hystrichopsylla dippiei
heotomae
0
0
0
0
0
0
0
0
0
0
0
35
0
0
0
0
0
0
0
0
2
0
3
3
0
6
0
2
0
2
10
1
1
1
1
0
2
Flea species
richness
0
2
0
0
0
0
0
0
0
0
0
3
0
0
0
0
0
0
0
Ceratophyllus ciliatus
mononis
Thrassis francisi
sierrae
0
0
0
9
38
0
1
0
0
0
0
3
0
0
0
0
0
3
0
Megabothris
abantis
0
0
0
0
0
0
0
0
0
0
0
94
0
0
0
0
0
0
0
Oropsylla
idahoensis
Number of individuals of each small mammal species infested with various species of fleas from YNP, California
0
0
0
5
6
0
116
0
0
0
1
5
0
0
1
0
0
1
1
Table 4.
Aetheca
wagneri
Number of individuals of each small mammal species infested with various species of fleas from YNP, California
Table 3.
104
Vol. 48, no. 1
January 2011
Table 5.
105
Small mammal-flea surveys of large sample size or conducted proximal to YNP, California
Locality
No. ea species
Citation
Hastings Reserve
San Diego County
Chuchupate
Plumas County
Yosemite
Morro Bay
Sagehen
Lava Beds
2,204
2,207
1,563
2,423
408
295
89
382
14
17
10
4
16
10
5
15
22,087
11,984
2,277
1,417
462
180
39
779
23
22
18
19
15
10
5
21
106
Table 6.
Lava
beds
Plumas
Sagehen
Yosemite
Hastings
Morro
Bay
198
171
1
Chuchupate
23
257
San
Diego
29
at least 2
215
11
267
14
4
19
1
23
18
10
22
6
123
81
2
1
10
1
1
1
28
198
28
4
14
206
4
10
26
26
121
14
1
22
1
1
8
3
1
2
16
607
3
44
1
4
11
131
1
14
50
4
391
1
2,516
7
8
237
8,353
3
4
251
1
224
37
45
44
22
8,192
15
792
7,537
101
28
18
21
35
94
56
14
19
17
1
310
695
17
607
1,806
2
63
93
21
333
5
2
10
168
6
93
11
6
20
27
18
847
2
1,417
131
5
1,053
156
1
4
21
72
39
6
66
462
22,087
293
204
567
13
117
1
180
2,277
11,948
Total
0
227
194
472
12
267
14
4
20
124
112
2
1
11
18
70
22
460
30
1
137
22
1
0
1
8
20
2
0
4
998
1
10,924
13
56
490
2
16
498
22
101
28
35
112
16,618
14
19
626
0
3,007
1
315
2
27
11
4
2,102
609
36
189
51
6
68
39,257
January 2011
Table 7.
107
Chaetodipus californicus
Glaucomys sabrinus
Marmota flaviventris
Microtus californicus
Microtus longicaudus
Microtus montanus
Peromyscus boylii
P. maniculatus
Peromyscus truei
Sciurus griseus
Spermophilus beecheyi
Spermophilus beldingi
Tamias alpinus
Tamias minimus
Tamias speciosus
Thomomys monticola
Zapus princeps
Total
Borrelia spp.
Rickettsia spp.
Yersinia spp.
No. positive
No. tested
No. positive
No. tested
No. positive
No. tested
No. positive
No. tested
0 (0%)
0 (0%)
1 (100%)
1 (50%)
3 (42.86)
6 (22.2)
0 (0%)
2 (1.53%)
0 (0%)
0 (0%)
1 (3.45%)
18 (18.56)
0 (0%)
0 (0%)
2 (28.57)
2 (40%)
6 (28.57)
42 (12.1%)
1
8
1
2
7
27
1
131
5
1
29
97
4
0
7
5
21
347
0 (0%)
3 (42.86%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
2 (25%)
8 (47.06)
1 (25%)
0 (0%)
6 (54.54%)
0 (0%)
0 (0%)
20 (37.03%)
0
7
1
0
0
0
0
0
0
1
8
17
8
1
11
0
0
54
0 (0%)
1 (16.67%)
0 (0%)
0 (0%)
1 (14.29%)
0 (0%)
1 (100%)
20 (28.57%)
0 (0%)
0 (0%)
4 (13.79%)
0 (0%)
0 (0%)
0 (0%)
3 (42.86%)
1 (20%)
3 (15.79%)
34 (13.93%)
1
6
1
1
7
24
1
70
5
1
29
62
5
0
7
5
19
244
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
0 (0%)
1
2
1
0
7
10
0
24
4
0
11
30
0
0
1
3
5
99
traps, and some species (e.g., voles and shrews) are more
amenable to trapping using other methods such as pitfall
traps. YNP has 33 rodent and shrew species listed within
its boundaries (www.nps.gov/yose/naturescience/
mammal-species-list.htm). We captured 16 of these in
our study, as well as two species of shrews. We did not
capture the nonnative Rattus rattus, Mus musculus, and
Castor canadensis. Several large native rodents, Erethizon dorsatum, Aplodontia rufa, Neotoma cinerea, Neotoma macrotis, Marmota flaviventris, and Spermophilus
lateralis, as well as the arboreal squirrels Sciurus griseus
and Tamiasciurus douglassii, also avoided our traps,
which is a problem that previously has been reported
(Nieto and Foley 2008). Thus, the species included in
this study could have resulted in an underestimate of
ectoparasite numbers and diversity, as well as disease
prevalence. Beldings ground squirrel and deer mouse
abundance may have been overrepresented because
of large population size and ease of capture.
Overall tick numbers observed on small mammals
were relatively low compared with other sites in California (Nieto et al. 2007, Adjemian et al. 2008, Foley
et al. 2008). North American deer mice had greatest
tick loads, possibly because they are such common
generalists present at nearly every location. The high
tick diversity on Beldings ground squirrels was previously unreported (Furman and Loomis 1984), including many species of nidicolous ticks (I. angustus,
Ixodes hearli, I. kingi, I. spinipalpis, and Ixodes woodi).
Chipmunks had no evidence of ticks, in contrast to our
published data in the Coast Range Mountains (Nieto
and Foley 2009). This may be because many of the
sites we sampled were relatively dry and at higher
altitude, which are inappropriate conditions for many
ticks, or because chipmunk capture numbers were
low. Small numbers of human-biting ticks (Ixodes
pacificus, I. angustus, Dermacentor andersoni) were
recovered, suggesting a possible risk of some tickborne disease in humans and their pets. However,
further work to evaluate the role of nidicolous and
specialist ticks in disease ecology is warranted. Spe-
108
January 2011
References Cited
Adjemian, J., E. Girvetz, and J. Foley. 2006. Analysis of the
GARP modeling approach for predicting the distributions
of eas implicated as vectors of plague, Yersinia pestis, in
California. J. Med. Entomol. 43: 93103.
Adjemian, J. Z., M. K. Adjemian, P. Foley, B. B. Chomel, R. W.
Kasten, and J. E. Foley. 2008. Evidence of multiple zoonotic agents in a wild rodent community in the eastern
Sierra Nevada. J. Wildl. Dis. 44: 737742.
Adjemian, J., J. Krebs, E. Mandel, and J. McQuiston. 2009.
Spatial clustering by disease severity among reported
Rocky Mountain spotted fever cases in the United States,
20012005. Am. J. Trop. Med. Hyg. 80: 7277.
Anderson, G. W., Jr., S. E. Leary, E. D. Williamson, R. W.
Titball, S. L. Welkos, P. L. Worsham, and A. M. Friedlander. 1996. Recombinant V antigen protects mice
against pneumonic and bubonic plague caused by F1-
109
110