CLS 422 Clinical Immunohematology I

Take Home Quiz

Day One

Working independently, select the best answer to the following questions. Submit your
answers via BlackBoard [Clinical Immunohematology I, Assignments, Student Lab, Rh
System Quiz One] before 8am of Day Two.

1.

The physical location of a gene on a chromosome is termed its:

a.
b.
c.
d.

2.

If a person has inherited the same gene from each parent, they are said to have
________________ gene expression.
a.
b.
c.
d.

3.

Genotype
Haplotype
Paternity
Phenotype

An antibody is a protein that:

a.
b.
c.
d.

5.

Codominant
Double
Heterozygous
Homozygous

By stating that a persons blood type is A Rh Positive, you are giving his:
a.
b.
c.
d.

4.

Allele
Locus
Phenotype
Site

Controls gene expression

Is found on the red blood cell (RBC) membrane
Is produced by plasma cells in response to a foreign antigen
Stimulates an immune response if recognized by the body as foreign

A hemolysin removes foreign matter by:

a.
b.
c.
d.

Acting as an opsonin to promote phagocytosis

Combining with the Fab portion of an antibody
Complement activation which leads to cell lysis
Enzyme activation after engulfing the foreign matter

CLS 422 Clinical Immunohematology I

Student Lab Day One Take Home Quiz

Page 1 of 6

6.

When performing an Immunohematology test by the tube method, the two

visible endpoints of antigen-antibody reactions are:
a.
b.
c.
d.

7.

An excess of antibodies in a test system leads to _____________ and a false

________________ reaction.
a.
b.
c.
d.

8.

Direct agglutination
Gel
Indirect antiglobulin test
Solid phase adherence

Which of the following is a disadvantage of monoclonal anti-serum?

a.
b.
c.
d.

11.

Anti-serum
Coombs Control Cells
LISS
Reagent RBCs

Which test method does not use intact RBCs to detect antibodies?
a.
b.
c.
d.

10.

Prozone / negative
Prozone / positive
Postzone / negative
Postzone / positive

To detect an antigen on a red blood cell membrane, the reagent of choice

would be:
a.
b.
c.
d.

9.

Agglutination and hemolysis

Aggregation and agglutination
Sensitization and agglutination
Sensitization and hemolysis

High rate of cross reactivity

Increased risk of infectious disease
Lot to lot variation in antibody strength
Possibility of missing a partial antigen

Broad spectrum anti-human globulin reagent (AHG) contains:

a.
b.
c.
d.

Antibodies to a wide variety of antigens

Anti-Fab and anti-Fc antibodies
Anti-mouse or anti-rabbit antibodies
Anti-IgG and Anti-C3 antibodies

CLS 422 Clinical Immunohematology I

Student Lab Day One Take Home Quiz

Page 2 of 6

12.

AHG reagent causes agglutination of RBCs by:

a. Coating the RBCs with IgG or C3
b. Cross-linking antibodies or complement components that are sensitizing
RBCs
c. Cross-linking antigens on opposing RBCs
d. Neutralizing antibodies in the patients serum

13.

The purpose of the wash step in antiglobulin testing is to:

a.
b.
c.
d.

14.

Of the following enhancement reagents, which one works by concentrating

the antibody, thereby increasing the chance of antigen/antibody interaction?
a.
b.
c.
d.

15.

Expose weakly expressed antigens.

Remove debris (broken glass, dust, etc) from the test tube.
Remove red blood cells that have not been sensitized.
Remove unbound antibodies.

Albumin
LISS
PEG
Enzymes

Which of the following best represents the genetic pathway of the ABO
system?
a. H gene------- H antigen------ ABO gene------ A, B or O antigens
b. Precursor------ H gene------- H antigen------ ABO gene------ A, B
or O antigens
c. Precursor------ H gene------- H antigen------ ABO gene------ A, B
or H antigens
d. Precursor------ h gene------- h antigen------ ABO gene------ A, B
or h antigens

16.

What is the terminal sugar that conveys Group B specificity?

a.
b.
c.
d.

D- galactose
Fucose
Glucose
N-acetylglucosamine

CLS 422 Clinical Immunohematology I

Student Lab Day One Take Home Quiz

Page 3 of 6

17.

If Amanda is Group A and Bob is Group B, their children could be:

a.
b.
c.
d.

18.

RBCs of which blood type possess the least amount of H antigen?

a.
b.
c.
d.

19.

A
AB
B
O

Which gene is responsible for the presence of A, B and/or H antigens in body

fluids?
a.
b.
c.
d.

20.

AB only
A or B
A, B, or AB
A, B, AB, or O

h
O
Se
se

A Group O individual will have which antibodies in his plasma?

a.
b.
c.
d.

Anti-A and anti-B

Anti-A, anti-B, and anti-A,B
Anti-H
No ABO antibodies present in the plasma

21.

T F

ABO antigens are fully developed at birth.

22.

T F

Antibodies in the ABO system are predominantly IgM.

23.

T F

ABO antibodies are naturally occurring.

24.

Interpret the following ABO test results:

Reagent: Anti-A Anti-B

Results: 0
4+
a.
b.
c.
d.
e.

A cells B cells
3+
0

A
B
AB
O
No interpretation discrepancy between forward and reverse grouping

CLS 422 Clinical Immunohematology I

Student Lab Day One Take Home Quiz

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25.

The Tippett theory of Rh inheritance proposes:

a. One gene producing three antigens inherited as a haplotype.
b. Two genes, one controlling D antigen production, the other controlling
production of C, c, E, and e antigens.
c. Two genes, Rh and Se.
d. Three closely linked genes inherited as a unit.

26.

The antigen with ISBT number 004002 is which antigen in Fisher-Race

nomenclature?
a.
b.
c.
d.
e.
f.

27.

D
d
C
c
E
e

Translate the Fisher-Race genotype DCe/dce into Wiener nomenclature.

a.
b.
c.
d.

R1R2
rr
R1r
Ro r

28.

T F

29.

Antibodies to the Rh system are usually demonstrable:

a.
b.
c.
d.

30.

Rh antigens are well developed at birth, and their expression may

be enhanced by enzymes.

At birth
In Rh positive individuals
Only after stimulation by foreign RBCs
Within 1 year of birth

Which of the following is a possible mechanism for exhibiting the weak D

phenotype?
a. Being of the R1 R1 genotype.
b. Inheriting the D gene from one parent and the d gene from the other
parent.
c. Missing a portion of the D molecule.
d. Possessing naturally occurring anti-D in the plasma.

CLS 422 Clinical Immunohematology I

Student Lab Day One Take Home Quiz

Page 5 of 6

31.

T F

Weak D testing should be performed on blood donors who test

negative with anti-D at the immediate spin phase.

32.

T F

The weak D test is an example of a direct antiglobulin test.

33.

Rh determinations are performed using the patients:

a.
b.
c.
d.

34.

Plasma and red blood cells

Plasma only
Red blood cells only
Saliva and RBCs

Interpret the results of the following Rh test performed using a high protein
reagent:

Test:
Results:
a.
b.
c.
d.

Anti-D
0

Weak D
0

CC
3+

Rh Control
0

Rh Negative
Rh Positive
Unable to interpret due to negative Rh control
Unable to interpret due to positive Coombs Control cells

CLS 422 Clinical Immunohematology I

Student Lab Day One Take Home Quiz

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