ANSWER SCHEME PAPER 3 MID YEAR F4 2012

Question 1 : 1(a)(i)

Score

Explanation
Able to state two correct observations based on following criteria.
C1 concentration of albumen
C2 time taken

3
Sample Answer:(either 2):

1. When the percentage of concentration of albumen suspension is 10%,

the time taken for enzyme reaction is 7 minutes.
2. When the percentage of concentration of albumen suspension is 20%,
the time taken for enzyme reaction is 13 minutes.
2

Able to state one correct observation and one inaccurate response.

Able to state one correct observation or two inaccurate response or

idea.

No response or wrong response (response like hypothesis)

1(a) (ii)

Score

Explanation
Able to state two reasonable inferences for the observation.

1(b)

Sample answer:

1. The rate of enzymatic reaction increases with the increase in

substrate concentration.
2. The rate of enzymatic reaction increases with the increase in
substrate concentration.
Able to state one correct inference and one inaccurate inference.

Able to state one correct inference or two inaccurate inference or

idea.

No response or wrong response (inference like hypothesis)

Score

Explanation
Able to record all readings of volume of urine produced

Percentage
concentration of
albumen
suspension

Time
taken/minutes

10%

15%

10

20%

13

Able to record any 2 corrects

Able to record any 1 correct

No response or wrong response

1(c) (i)

Score
3

Explanation
Able to state all the variables and the method to handle variable
correctly
() for each variable and method
Manipulated Variable: Concentration of albumen suspension

()

Method to handle: Use different concentration of albumen suspension

()

Responding Variable: Time taken for the digestion of albumen ()

Method to handle: Observed and record the time taken for albumen
suspension become
colorless by using stopwatch ()

Controlled variable : Enzyme concentration, temperature and pH of

medium ()

Method to handle: . Fix the enzyme concentration to 1%. Use 1%

pepsin, 1 ml of pepsin ()

Able to get 6 (with the correct key words)

Able to get 4 5
1

Able to get 2 3

No response or wrong response

1(c)(ii)

Score

Explanation
Able to classify the apparatus and material used in the experiment

Apparatus

Materials

Syringe

Albumen suspension

Stopwatch

Pepsin

Thermometer

Water bath

Able to classify all the apparatus and material correctly.

2

Able to classify two apparatus and one material correctly.

Able to classify one apparatus and one material correctly.

No response or wrong response

1(d)

Score
3

Explanation
Able to state the hypothesis correctly based on the following
criteria:
V1 State the substrate concentration
V2 State the enzyme reaction
R - State the relationship between V1 and V2.

The rate of enzymatic reaction increases with the increase in

substrate concentration
2

Able to state the hypothesis but less accurate.

Able to state the idea of the hypothesis

No response or wrong response

1(e)(i)

Score

Explanation
Able to construct a table and record the result of the experiment
with the following criteria:
percentage concentration of albumen suspension (%)
Time (minutes)
- Rate of enzymatic reaction as percentage of albumen
concentration per minute

Percentage
concentration
of albumen
suspension (%)

Time/minut
es

10

Rate of enzyme reaction as

percentage of albumen
concentration per minute
10/7
= 1.43

14

10

15/10
= 1.50

20

13

20/13
= 1.54

Able to construct a table and record any two criteria

Able to construct a table and record any one criteria

No response or wrong response

1(e)(ii)

Score
3

Explanation
Able to draw a graph of rate of reaction of pepsin against the
concentration of albumen suspension

P all axis with uniform scale and correct units

T all points transferred correctly
B smooth graph was plotted
2

Graph with any two criteria.

Graph with any one criteria.

No response or wrong response.

1(e)(iii)

Score

Explanation
Able to explain the relationship between the concentration of
albumen suspension and the rate of reaction of pepsin.

The rate of pepsin reaction increases with the percentage of the

concentration of albumen suspension and it reaches a maximum
value

Able to explain briefly the relationship between the concentration of

albumen suspension and the rate of reaction of pepsin.

Able to explain the idea the relationship between the concentration

of albumen suspension and the rate of reaction of pepsin.

No response or wrong response

1(f)

Score
3

Explanation
This enzyme, which is Pepsin acts on albumen suspension to change
it into clear solution. The rate of enzyme reaction increases with the
substrate concentration.

1(g)

Score

Explanation
R. The 1 ml of enzyme used is boiled enzyme, hence it is
denatured/destroyed and cannot act on the substrate.

MARKING SCHEME
QUESTION 2

1. Aim of investigating
Sample answer

Remarks

Able to state the objective of investigation correctly.

Sample answer:

Tick

1) To study the concentration of sucrose solution which is

isotonic to the cell sap of papaya slices
2) To determine the effect of different concentration of
sucrose solution to the length of papaya slices.

2. Problem statement
Score
3

Explanation
Able to state the problem statement relating the
manipulated variable with responding variable
correctly.
Sample answer :
1) What is the concentration of sucrose
solution which is isotonic to the cell sap of
papaya slices?
2) What is the effect of difference of sucrose
solution to the length of papaya slices?

Able to state the problem statement but slightly

incorrect.
Sample answer :

Remarks
One
P1concentration
of sucrose
solution

P2- final
length of
papaya slices
One
P1, H only

1) What is the effect of concentration of

sucrose solution to the length of papaya
slices?
2) What is the concentration of sucrose
solution which is isotonic to the cell sap of
papaya slices?
1

Able to state the idea only.

P2, H only
P1, P2 only

One

Sample answer :

P1,P2, H,

Sucrose solution affects the length of papaya

slices.

No question
marks.

No response or wrong response.

3. Hypothesis
Score
3

Explanation
Able to state the hypothesis correctly by relating
the two variables correctly.
Sample answer :

Remarks
One
Must have P1,
P2 and H

1) When the sucrose solution is isotonic to the

cell sap of plant cells, there is no net gain in
size.
2) The higher the concentration of sucrose
solution, the lesser the final length of papaya
slices.
2

Able to state the hypothesis but slightly incorrect.

Sample answer :
1) The isotonic solution has no effect on the
plant cell sap on the length of papaya slices.
2) The different of concentration of sucrose
solution influence the final length of papaya
slices

Able to state the idea only.

One
P1, P2
P1, H
P2, H

One

Sample answer :
1) The concentration of sucrose solution affect
the plant cell sap
0

No response or wrong response.

4. Variables
Sample answer
Able to state ALL three variables correctly.

Remarks
One

Manipulated : Concentration of sucrose solution

Responding : Final length of the papaya slices
Fixed

: Initial length of the papaya slices

5. List of materials and apparatus

Score
3

Explanation
Able to state all the materials and apparatus
correctly.
Sample answer :
Materials :
and

papaya , distilled water, filter paper

sucrose solutions with different
concentrations

Apparatus:

Test tubes/ petri dishes, knife, forceps,

ruler and cork borer.

Able to state 3 materials and 3 apparatus.

Remarks
One

Able to state 2 materials and 2 apparatus.

No response or wrong response.

6. Technique
Sample answer

Remarks

Measuring the lengths of papaya slices with a ruler and record

Bonus 1 mark
One

7. Procedure
Score

Explanation

Remarks

Able to list down the complete procedures based

on the following five criteria, K1, K2, K3, K4 and K5.

One

K1 : Procedures of assembling the materials and

apparatus.
(Step 1 5)
K2 : Method to operate manipulated variable.
(Step 3)
K3 : Method to determine responding variable.
(Step 6)
K4 : Method of controlling variable.
(Step 3, 4 & 6)
K5 : Method to take precaution steps.
(Step 6)

Sample answer :
1.
Seven test tubes are labelled as A, B, C, D, E,
F and G.
2.
Test tube A is filled with 25ml of distilled
water.
3.
Each test tube is filled with 25 ml of different
solutions as shown as below :
Test tube B : 0.1M sucrose solution
Test tube C : 0.2M sucrose solution
Test tube D : 0.3M sucrose solution
Test tube E : 0.4M sucrose solution
Test tube F : 0.5M sucrose solution
Test tube G : 0.6M sucrose solution
4. A cork borer is used to bore 21 papaya slices.
Each slice is cut to a length of 5 cm.
5.
Three slice are placed in each test tube and
left for an hour.
6. After an hour, the slice are removed. The
papaya slices are wiped dry and the length of each
slice is measured. The measurements are recorded.
7. A graph of the change in length of the papaya
slices against the concentrations of sucrose
solution is plotted.

Able to state 3 to 4 criteria.

Able to state at least 2 criteria.

No response or wrong response.

8. Presentation of data / result

Able to construct a table based on the following criteria.

1) concentration of sucrose solution
2) The initial and final length of papaya slices

Two

3) The change in the length of papaya slices

Concentration

Initial length (cm)

Final length (cm)

Change

of sucrose

in

solution (M)

length
1

Mean

Mean

Bonus 1 mark

(cm)

0 (Distilled
water)
0.1
0.2
0.3
0.4
0.5
0.6
Predicted graph

Change in length
(cm)

Graph of change in length of papaya slices against concentration of sucrose solution

0.1

0.2

0.3

0.4

0.5

9. Conclusion
Sample answer

0.6
Concentration of
sucrose solution
(M)

Remarks

State the hypothesis.

Hypothesis accepted

One

Aspect 3 : Planning
7 9 = 3 marks
4 6 = 2 marks
1 3 = 1 marks

Summary
Problem statement
Hypothesis

= 3 marks
= 3 marks

Materials & apparatus

= 3 marks

Procedures

= 3 marks

Planning

= 3 marks

Bonus

= 2 marks

Total

= 17 marks