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On: 27 December 2014, At: 00:39
Publisher: Taylor & Francis
Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House,
37-41 Mortimer Street, London W1T 3JH, UK
Mohammad Moghaddam , Maryam Pourbaige , Heydar Kourosh Tabar , Nasrin Farhadi &
Seyed Mohammad Ahmadi Hosseini
Chemistry and Chemical Engineering Research Center of Iran, 657, Soheil Shiraz, Vanak,
Tehran, Iran
d
To cite this article: Mohammad Moghaddam, Maryam Pourbaige, Heydar Kourosh Tabar, Nasrin Farhadi & Seyed Mohammad
Ahmadi Hosseini (2013) Composition and Antifungal Activity of Peppermint (Mentha piperita) Essential Oil from Iran, Journal
of Essential Oil Bearing Plants, 16:4, 506-512, DOI: 10.1080/0972060X.2013.813265
To link to this article: http://dx.doi.org/10.1080/0972060X.2013.813265
506
ISSN Print: 0972-060X
ISSN Online: 0976-5026
Abstract: Essential oil of Mentha piperita was analyzed by GC and GC-MS and evaluated for in vitro
antifungal activity against Dreschlera spicifera, Fusarium oxysporum f.sp. ciceris and Macrophomina
phaseolina. Thirty-five components have been identified in the essential oil of M. piperita. The major compounds
identified in the oil were menthone (30.63 %), menthol (25.16 %), menthofuran (6.47 %), -phellandrene
(5.59 %), isomenthone (4.74 %), menthol acetate (4.61 %), pulegone (4.39 %), -caryophyllene (3.05 %),
neomenthol (2.83 %), 1,8-cineole (2.15 %). The antifungal assay was determined by agar dilution method with
five concentrations of peppermint oil (up to 1600 ppm). The oil was found to be effective against these fungal
pathogens under laboratory screening. The antifungal activities of the oil increased with an increase in the
concentration. Minimum effective concentrations of the oil against fungal pathogens were also different. The
fungistatic and fungicidal activity of the oil was determined. Results of fungistatic and fungicidal activities
showed that peppermint oil in these concentrations have no fungicide activity.
Key words: Mentha piperita; essential oil composition; antifungal activity; menthone; menthol.
Introduction
Pathogens fungi contaminate crops and foods
and cause significant yield reduction and
economic losses. Synthetic chemicals with
various degree of persistence are employed as
fungicide in crop protection. The use of such
fungicides, although increased crop production
but with some deterioration of environmental
quality and human health 1,2,3. Furthermore with
the target pathogen, pesticides may also kill
*Corresponding author (Mohammad Moghaddam)
E-mail: < m.moghadam@ferdowsi.um.ac.ir >
507
Statistical analyses
The experiment was repeated three times for
each fungus and for each dose. The significance
of the findings was determined by variance
analysis (ANOVA) by using SPPS 10.0 software
package.
Results and Discussion
The results of the oil analyses are given in Table
1. The essential oil content of peppermint leaves
was 1.38 % (w/w), based on the dry weight of
the plant. Thirty-five compounds were identified
in the essential oils of M. piperita. The major
compounds were menthone (30.63 %), menthol
(25.16 %), menthofuran (6.47 %), -phellandrene
(5.59 %), isomenthone (4.74 %), menthol acetate
(4.61 %), pulegone (4.39 %), -caryophyllene
(3.05 %), neomenthol (2.83 %), 1,8-cineole (2.15
%), germacrene D (1.87 %), trans-sabinene
Component
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
-Thujene
-Pinene
Sabinene
-Pinene
3 Octanol
Myrcene
-Terpinene
p-Cymene
1,8-Cineole
-Phellandrene
(Z)--ocimene
-Terpinene
trans-Sabinene hydrate
Linalool
enthone
Isomenthone
Menthofuran
Neomenthol
Menthol
Isomenthol
-Terpineol
Pulegone
Piperitone
Neomenthol acetate
508
RIa
Percentage
922
930
965
970
978
981
1007
1010
1019
1020
1024
1048
1053
1082
1142
1149
1153
1155
1168
1174
1177
1219
1229
1261
0.05
0.85
0.62
1.26
0.05
0.17
0.09
0.14
2.15
5.59
0.02
0.17
1.53
0.14
30.63
4.74
6.47
2.83
25.16
0.24
0.36
4.39
0.48
0.28
509
table 1. (continued).
No.
Component
25
26
27
28
29
30
31
32
33
34
35
Menthol acetate
Isomenthol acetate
-Bourbonene
-Elemene
-Caryophyllene
(Z)--farnesene
-Humulene
Germacrene D
Germacrene A
Caryophyllen oxide
Viridiflorol
Total
RIa
Percentage
1280
1295
1388
1390
1425
1446
1457
1483
1496
1578
1590
4.61
0.14
0.23
0.15
3.05
0.39
0.13
1.87
0.4
0.19
0.4
99.97
2.3 0.15
00.00 4.70.76
15.31.24 1.20.69 13.22.53
45.81.28 57.90.39
572.13
73.10.56 80.51.05 1000.00
1000.00 93.40.18 1000.00
00.00
00.00
00.00
8.40.87 1.40.39 7.62.41
290.56 2.81.04 20.36.21
66.32.4 91.50.35 65.54.89
87.60.86 94.91.03 1000.00
1000.00 1000.00 1000.00
00.00
00.00
00.00
13.90.78 3.40.73 10.81.72
32.50.98 12.41.17 22.11.26
72.46.27 95.30.83 67.85.12
1000.00 97.10.80 1000.00
1000.00 1000.00 1000.00
00.00
00.00
00.00
100
200
400
800
1600
Control
Day2
B
Day7
B
510
511
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