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Faculdade de Cincias Agrrias e Veterinrias, UNESP Univ Estadual Paulista. Via de Acesso Prof. Paulo Donato
Castellane s/n 14884-900 - Jaboticabal, SP, Brasil.
2
Escola de Engenharia de Lorena, USP - Universidade de So Paulo. Estrada Municipal do Campinho s/n, CEP 12602810, Lorena, SP, Brasil.
Received 21 November, 2014; Accepted 2 February, 2015
The use of vegetable biomass as substrate for ethanol production could reduce the existing usage of
fossil fuels, thereby minimizing negative environmental impacts. Due to mechanical harvesting of
sugarcane, the amount of pointer and straw has increased in sugarcane fields, becoming inputs of great
energy potential. This study aimed to analyze the use of hemicellulosic hydrolyzate produced by
sugarcane pointers and leaves compared with that of sugarcane juice fermented by yeasts that unfold
hexoses and pentoses in the production of second generation biofuel, ethanol. The substrates used for
ethanol production composed of either sugarcane juice (hexoses) or hemicellulosic hydrolyzate from
sugarcane leaves and pointers (pentoses and hexoses), and the mixture of these two musts.
Fermentation was performed in a laboratory scale using the J10 and FT858 yeast strains using 500 ml
7
Erlenmeyer flasks with 180 ml of must prepared by adjusting the Brix to 16 0.3; pH 4.5 0.5; 30C; 10
CFU/ml with constant stirring for 72 h, with four replications. Cell viability, budding, buds viability, and
ethanol production were evaluated. Among the yeasts, the cell viability was greater for J10. The use of
FT858 + J10 was effective in producing ethanol. The hemicellulosic hydrolyzate had low efficiency in
ethanol production compared with sugarcane juice.
Key words: Hydrolysis of sugarcane straw and pointers, sugarcane juice, xylose, cell viability, ethanol.
INTRODUCTION
With the decline in world oil reserves, along with price
instability and the appeal for the sustainable use of
natural resources, the search for alternative such as
520
Afr. J. Biotechnol.
Roviero et al.
521
Table 1. Cellulose, hemicellulose and lignin from the straw and sugarcane tip
before and after hydrolysis of the hemicellulose fraction.
Percentage
Composition before acid hydrolysis
Composition after acid hydrolysis
Cellulose
37.32
22.44
Hemicellulose
35.98
28.71
Lignin
5.52
11.80
Evaluated parameters
Brix
pH
Sulfuric Acid Concentration (g/L)
Phenolic Compounds (g/L)
Total Monosaccharides (g/L)
SJM
16.1
4.51
0.73
0.17
111.7
HHM
16
4.24
5.72
2.85
81
HSJM
16.3
4.31
2.69
1.82
97.5
Values are represented as means. SJM, Sugarcane juice; HHM, hemicellulosic hydrolyzate sugarcane
leaves and pointers; HSJM, the mixture of these two substrates.
Statistical analysis
The results of cell viability and ethanol production were subjected to
analysis of variance by the F test, and the comparison of the means
was performed by the Tukey test (Barbosa and Maldonado, 2011).
522
Afr. J. Biotechnol.
Table 3. Variance analysis and comparison of means by the Tukey test (5%
probability) of the microbiological analysis results using the musts composed of
sugarcane juice (SJM), hemicellulosic hydrolyzate (HHM), and sugarcane juice
+ hemicellulosic hydrolyzate (HSJM), with the strains J10, FT858, and J10 +
FT858.
88.69 *
C
68.89
B
78.59
83.77
C
73.84
B
78.55
4.66**
Budding (%)
7.57
B
3.67
A
5.77
87.46
C
63.54
B
77.71
80.27
B
69.85
A
78.58
ns
2.80
6.14
A
5.45
A
5.42
15.04**
Roviero et al.
523
Figure 1. Graphical representation of the unfolding of the musts and yeasts (J10. FT858 and J10 +
FT858) over a 72 h period for ethanol production.
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Afr. J. Biotechnol.
Conflict of interests
The authors did not declare any conflict of interest.
ACKNOWLEDGMENTS
CAPES is acknowledged for the master scholarship
granted to the first author.
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