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UV-Visible/NIR
Spectrophotometer
Fluorescence
Spectrophotometer
Qualitative analysis
A standard sample of aspartame was measured, yielding a peak at 12.5 minutes. This
peak corresponds to aspartame, itself.
Next,
a sample prepared from a beverage was measured under the same conditions,
yielding a number of peaks. The peak appearing at 12.5 minutes can be regarded as
that of aspartame (Figure 1).
The following conditions were the same: the type of fillers, column sizes, column
temperature, composition of the mobile phase, and flow rate.
Atomic Absorption
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Quantitative analysis
The height and area of a peak are proportional to the concentration of the corresponding
component. A calibration curve is created using the standard sample. The
concentration
of aspartame in the beverage can be determined from the peak area of the detected
aspartame.
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HPLC Basic Course 2.Principle and System Configuration of HPLC(2):Hitachi High-Technologies Corporation
Attention should be given to the fact that a qualitative analysis includes many
uncertainties. Other components may have been eluted together with aspartame.
LC and GC systems are good at determining the content of a
certain component in a
sample, rather than the types of the components of a sample. Performance of a
quantitative analysis requires the preparation of a calibration curve. It is very difficult to
perform the qualitative or quantitative analysis of a component for which a standard
sample is not available.