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Rule of CORN
7 withdraw
Liver
4 withdraw
Eye
3 withdraw
1980-2005
Others
6 withdraw
Helix pitch 34
Diameter 20
10.4 base per turn
In contrast, helix has a
pitch of 5.4 and radius
of 10 . 3.6 residues
per turn.
Sequencing DNA
Prior to the mid-1970s no method existed by
which DNA could be directly sequenced.
Knowledge about gene and genome organization
was based upon studies of prokaryotic organisms
and the primary means of obtaining DNA sequence
was so-called reverse genetics in which the amino
acid sequence of the gene product of interest is
back-translated into a nucleotide sequence based
upon the appropriate codons.
Microarray
The relative concentration of any given
mRNA in a population of transcripts can
be determined by hybridization to its
specific complementary sequences.
The relative abundance of each of the
mRNAs encoded in a given genome can
therefore be assessed by their
simultaneous hybridization to the complete
set of corresponding sequence.
1. Prepare your
DNA chip using
your chosen target
DNAs.
3. Incubate your
hybridization
mixture containing
fluorescently
labeled cDNAs
2. Generate a
with your DNA
hybridization
solution containing chip.
a mixture of
fluorescently
labeled cDNAs.
4. Detect bound
cDNA using laser
technology and
store data in a
computer.
5. Analyze data
using
computational
methods.
http://www.dnalc.org/ddnalc/resources/dnachip.html
Note: Thanks to Prof. Vishy Iyer for many of these slides on microarrrays.
To conduct microarray
hybridization, which of this
ingredient is needed?
A
B.
C.
D.
E.
Mg ion
cDNA pieces
dNTP
ddNTP
DNA polymerase
3. Mutation
4. Function assignment for unknown proteins